Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,078,245	C→G	G470G (GGG→GGC)	tktA ←	transketolase 1 isozyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,078,245	0	C	G	100.0%	664.6 / NA	216	G470G (GGG→GGC)	tktA	transketolase 1 isozyme
Reads supporting (aligned to +/- strand): ref base C (0/0); new base G (107/109); total (107/109)

Only 100 of 233 total aligned reads displayed.

GCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGCCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGTGC  >  NC_000913/3078156‑3078334

gcgGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCg                                                                                        >  2:2930832/1‑93 (MQ=255)

  ggATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTc                                                                                      <  1:5285087/93‑1 (MQ=255)

    aTTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCtt                                                                                    <  2:1847021/93‑1 (MQ=255)

     ttCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTc                                                                                   >  1:5059900/1‑93 (MQ=255)

        aaCCTGGTCACACGGACGCCATGTAGATATGTTCGGGGTTACGCGCAGCGAAGCGACCTGGTCAACCGGCTGGTGAGTCGGGCCGtcgtcggc                                                                                >  2:4008011/1‑91 (MQ=255)

         aCCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGccc                                                                               >  1:5211776/1‑93 (MQ=255)

         aCCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGccc                                                                               >  1:5832154/1‑93 (MQ=255)

         aCCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGccc                                                                               >  2:5684709/1‑93 (MQ=255)

         aCCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGGCTTCTccc                                                                               <  2:3042341/93‑1 (MQ=255)

          ccTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCa                                                                              >  2:2002316/1‑93 (MQ=255)

           cTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAg                                                                             >  1:5485569/1‑93 (MQ=255)

            tGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGTAGAGAAGCGACCTGCTCAACCGGCCGGTGAGTCGGGCCGTCTTCGCCCAGa                                                                            >  1:6308602/1‑93 (MQ=255)

            tGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGa                                                                            <  1:5011463/93‑1 (MQ=255)

            tGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGa                                                                            >  1:5398536/1‑93 (MQ=255)

             ggTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGAc                                                                           >  2:2463319/1‑93 (MQ=255)

                cacaCGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGa                                                                        <  1:80790/93‑1 (MQ=255)

                cacaCGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGa                                                                        >  2:5892922/1‑93 (MQ=255)

                cacaCGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGa                                                                        >  2:3281123/1‑93 (MQ=255)

                 acacGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGAt                                                                       >  1:3017138/1‑93 (MQ=255)

                 acacGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGAt                                                                       >  1:4342576/1‑93 (MQ=255)

                 acacGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGAt                                                                       <  2:5614237/93‑1 (MQ=255)

                   acGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATgg                                                                     <  2:4861614/93‑1 (MQ=255)

                    cGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGa                                                                    <  1:3585015/93‑1 (MQ=255)

                     ggACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGCTGGAg                                                                   >  1:771855/1‑93 (MQ=255)

                     ggACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGat                                                                   <  2:1162326/93‑2 (MQ=255)

                        cGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCg                                                                <  1:3147495/93‑1 (MQ=255)

                          ccATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTg                                                              <  1:1512220/93‑1 (MQ=255)

                           cATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTgg                                                             >  1:4227901/1‑93 (MQ=255)

                           cATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGACGTCTTCGCCCAGACCGATGGAGTCGTgg                                                             >  2:3104668/1‑93 (MQ=255)

                                aGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCACCCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTa                                                        <  1:3205429/93‑1 (MQ=255)

                                aGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTa                                                        <  1:1630264/93‑1 (MQ=255)

                                 gACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTaa                                                       >  1:1056684/1‑93 (MQ=255)

                                 gACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTaa                                                       >  2:4746883/1‑93 (MQ=255)

                                 gACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTaa                                                       <  1:3870612/93‑1 (MQ=255)

                                  aCATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTaaa                                                      <  1:3880145/93‑1 (MQ=255)

                                  aCATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTaaa                                                      <  1:4980875/93‑1 (MQ=255)

                                  aCATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGCGCCGTCTTCGCCCAGACCGATGGAGGCGTGGGTGTaaa                                                      >  2:3526781/1‑93 (MQ=255)

                                   cATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAAc                                                     <  2:172949/93‑1 (MQ=255)

                                   cATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAAc                                                     <  1:3436152/93‑1 (MQ=255)

                                    aTGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAAcc                                                    >  1:2555562/1‑93 (MQ=255)

                                    aTGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAAcc                                                    >  2:604476/1‑93 (MQ=255)

                                     tGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCa                                                   <  2:5475381/93‑1 (MQ=255)

                                     tGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCa                                                   <  2:2608999/93‑1 (MQ=255)

                                     tGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCa                                                   >  2:898753/1‑93 (MQ=255)

                                     tGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGCGTAAACCa                                                   >  1:5473422/1‑93 (MQ=255)

                                      gTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCAt                                                  <  1:610259/93‑1 (MQ=255)

                                      gTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGGCGTGGGTGTAAAcact                                                  >  1:1967963/1‑90 (MQ=255)

                                      gTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAAACGGCTGTTGAGTCCGGCCGTCTTCGCCCAGAGCGATGGAGGCGTGGGCGCAAACCAt                                                  >  2:2768830/1‑93 (MQ=255)

                                        tCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGCGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTCTGTGTAAACCATCa                                                >  2:5238891/1‑93 (MQ=255)

                                          ggggTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGACGTCTTCGCCCAGACCGGTGGAGTCGTGGGGGGAAAACATccac                                              >  1:2199779/1‑90 (MQ=255)

                                           gggTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCt                                             <  1:2612680/93‑1 (MQ=255)

                                           gggTTACGCGCAGAGAAGCGACCTGCTCAACCCGCTGGGGAGTCGGGCCGCCTTCGACCAGACCAATGGAGAAGTAGGTGGATAACATCACCt                                             >  2:4524823/1‑93 (MQ=255)

                                           gagTTAGGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGATTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAACCCATCCCCt                                             <  2:6093614/91‑1 (MQ=255)

                                             gTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGa                                           <  1:1742059/93‑1 (MQ=255)

                                             gTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGCCGTGGGTGTAAACCATCACCTgc                                           >  2:4487636/1‑92 (MQ=255)

                                              ttACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGAc                                          <  2:665530/93‑1 (MQ=255)

                                              ttACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGAc                                          >  1:32621/1‑93 (MQ=255)

                                              ttACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGCGAGTCGGGCCGGCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGAc                                          >  2:2162970/1‑93 (MQ=255)

                                                  gcgcAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTg                                      <  1:3632229/93‑1 (MQ=255)

                                                  gcgcAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTg                                      >  2:5002366/1‑93 (MQ=255)

                                                  gcgcAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCCTCGCCCAGACCGATGGCGCCGTGGGCGCAAACCATCACCTGACGCTg                                      >  2:2298273/1‑93 (MQ=255)

                                                    gcAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGtt                                    <  2:1001273/93‑1 (MQ=255)

                                                     cAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGttt                                   <  2:934996/93‑1 (MQ=255)

                                                     cAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGttt                                   <  1:245672/93‑1 (MQ=255)

                                                     cAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGttt                                   <  1:4376142/93‑1 (MQ=255)

                                                      agagAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTtc                                  <  2:1955136/93‑1 (MQ=255)

                                                      agagAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTtc                                  >  2:3799560/1‑93 (MQ=255)

                                                       gagaAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTtca                                 <  2:5268790/93‑1 (MQ=255)

                                                        agaAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACCCTGTTtcat                                >  2:6292474/1‑93 (MQ=255)

                                                         gaAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTtcatc                               >  1:1543724/1‑93 (MQ=255)

                                                         gaAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTtcatc                               >  2:1416852/1‑93 (MQ=255)

                                                         gaAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTtcatc                               >  2:5771513/1‑93 (MQ=255)

                                                         gaAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTtcatc                               >  1:5866488/1‑93 (MQ=255)

                                                          aaGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTtcatca                              >  2:4653869/1‑93 (MQ=255)

                                                             cGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAgcg                           <  1:725603/93‑1 (MQ=255)

                                                              gACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAgcgc                          >  2:6272519/1‑93 (MQ=255)

                                                              gACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAgcgc                          <  1:3369765/93‑1 (MQ=255)

                                                               aCCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCa                         >  1:2467333/1‑93 (MQ=255)

                                                               aCCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCa                         <  2:2344889/93‑1 (MQ=255)

                                                                ccTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAg                        >  1:124444/1‑93 (MQ=255)

                                                                ccTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAg                        >  2:780856/1‑93 (MQ=255)

                                                                 cTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGc                       <  2:2698226/93‑1 (MQ=255)

                                                                 cTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGc                       >  1:2804085/1‑93 (MQ=255)

                                                                  tacTCAACCGCCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGcc                      <  2:5853326/91‑1 (MQ=255)

                                                                   gCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCa                     >  1:3664054/1‑93 (MQ=255)

                                                                   gCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCa                     >  2:3725090/1‑93 (MQ=255)

                                                                    cTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCAt                    >  1:1804702/1‑93 (MQ=255)

                                                                       aaCTGGCTGGTGAGTCCGGGCGTCTTCGCCCAGACCGAGGGAGGCGTGGGGGTAAGCTCTAAGCTCACGCTGTTTAACTAGCGCAGCCAtgcg                 >  2:1799290/1‑93 (MQ=255)

                                                                        aCCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCAtacgt                <  2:6194174/93‑1 (MQ=255)

                                                                         ccGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCAtacgta               <  1:5918128/93‑1 (MQ=255)

                                                                         ccGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCAtacgta               <  1:4720006/93‑1 (MQ=255)

                                                                          cGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCAtacgtac              <  1:811314/93‑1 (MQ=255)

                                                                           ggCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCAtacgtacg             <  2:4356411/93‑1 (MQ=255)

                                                                           ggCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCAtacgtacg             >  2:4154087/1‑93 (MQ=255)

                                                                             cTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGc           <  1:4549948/93‑1 (MQ=255)

                                                                               ggTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGt         >  2:3380059/1‑93 (MQ=255)

                                                                                  gAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTAc      <  2:3963529/93‑1 (MQ=255)

                                                                                  gAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTAc      <  1:1152634/93‑1 (MQ=255)

                                                                                    gTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGt    <  1:1204117/93‑1 (MQ=255)

                                                                                      cGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGTGc  <  2:4415604/93‑1 (MQ=255)

GCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGCCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGTGC  >  NC_000913/3078156‑3078334

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGCCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGTGCGT  >  NC_000913/3078152‑3078336

GACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGG                                                                                             <  SRR4302533.1196153/94‑1 (MQ=60)

GACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGG                                                                                             >  SRR4302533.2023499/1‑94 (MQ=60)

      GGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCT                                                                                       >  SRR4302533.901106/1‑94 (MQ=60)

      GGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCT                                                                                       <  SRR4302533.5316745/94‑1 (MQ=60)

        ATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTC                                                                                     >  SRR4302533.5090475/1‑94 (MQ=60)

         TTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCG                                                                                    >  SRR4302533.5019321/1‑94 (MQ=60)

            AACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCC                                                                                 >  SRR4302533.5867402/1‑94 (MQ=60)

            AACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCC                                                                                 >  SRR4302533.5243162/1‑94 (MQ=60)

             ACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCA                                                                                <  SRR4302533.5876452/94‑1 (MQ=60)

             ACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCA                                                                                >  SRR4302533.1886661/1‑94 (MQ=60)

              CCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAG                                                                               <  SRR4302533.6073546/94‑1 (MQ=60)

              CCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAG                                                                               >  SRR4302533.5518633/1‑94 (MQ=60)

               CTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGTAGAGAAGCGACCTGCTCAACCGGCCGGTGAGTCGGGCCGTCTTCGCCCAGA                                                                              >  SRR4302533.6346478/1‑94 (MQ=60)

               CTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGA                                                                              >  SRR4302533.5430975/1‑94 (MQ=60)

                TGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGAC                                                                             <  SRR4302533.5041827/94‑1 (MQ=60)

                 GGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACC                                                                            <  SRR4302533.437476/94‑1 (MQ=60)

                    CACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGAT                                                                         <  SRR4302533.81304/94‑1 (MQ=60)

                    CACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGAT                                                                         >  SRR4302533.3035592/1‑94 (MQ=60)

                    CACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGAT                                                                         >  SRR4302533.4368746/1‑94 (MQ=60)

                     ACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATG                                                                        >  SRR4302533.5176764/1‑94 (MQ=60)

                       ACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGA                                                                      <  SRR4302533.4464937/94‑1 (MQ=60)

                        CGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGCTGGAG                                                                     >  SRR4302533.776395/1‑94 (MQ=60)

                        CGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAG                                                                     <  SRR4302533.3607095/94‑1 (MQ=60)

                          GACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTC                                                                   <  SRR4302533.4819680/94‑1 (MQ=60)

                            CGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGT                                                                 <  SRR4302533.3899577/94‑1 (MQ=60)

                            CGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGT                                                                 <  SRR4302533.3166847/94‑1 (MQ=60)

                            CGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGT                                                                 <  SRR4302533.1254885/94‑1 (MQ=60)

                            CGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGT                                                                 <  SRR4302533.2904544/94‑1 (MQ=60)

                              CCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGG                                                               >  SRR4302533.4253463/1‑94 (MQ=60)

                              CCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGG                                                               >  SRR4302533.3626018/1‑94 (MQ=60)

                              CCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGG                                                               <  SRR4302533.3229391/94‑1 (MQ=60)

                              CCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGG                                                               <  SRR4302533.5988183/94‑1 (MQ=60)

                              CCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGG                                                               >  SRR4302533.2568306/1‑94 (MQ=60)

                              CCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGG                                                               <  SRR4302533.1521333/94‑1 (MQ=60)

                              CCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACAGATGGAGTCGTGG                                                               >  SRR4302533.2024112/1‑94 (MQ=60)

                               CATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGG                                                              <  SRR4302533.2821868/94‑1 (MQ=60)

                               CATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGG                                                              >  SRR4302533.149090/1‑94 (MQ=60)

                                ATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGG                                                             >  SRR4302533.2444112/1‑94 (MQ=60)

                                    AGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCACCCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAA                                                         <  SRR4302533.3225163/94‑1 (MQ=60)

                                    AGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAA                                                         >  SRR4302533.1062897/1‑94 (MQ=60)

                                    AGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAA                                                         <  SRR4302533.1640141/94‑1 (MQ=60)

                                     GACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAA                                                        <  SRR4302533.3894239/94‑1 (MQ=60)

                                      ACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAAC                                                       <  SRR4302533.270147/94‑1 (MQ=60)

                                      ACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAAC                                                       >  SRR4302533.5060978/1‑94 (MQ=60)

                                      ACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAAC                                                       <  SRR4302533.5011073/94‑1 (MQ=60)

                                      ACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAAC                                                       <  SRR4302533.3903819/94‑1 (MQ=60)

                                       CATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACC                                                      >  SRR4302533.2571281/1‑94 (MQ=60)

                                       CATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACC                                                      <  SRR4302533.3457380/94‑1 (MQ=60)

                                        ATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGCGTAAACCA                                                     >  SRR4302533.5506412/1‑94 (MQ=60)

                                         TGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGGCGTGGGTGTAAACact                                                    >  SRR4302533.1979994/1‑91 (MQ=60)

                                          GTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATC                                                   <  SRR4302533.3100094/94‑1 (MQ=60)

                                          GTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATC                                                   <  SRR4302533.613847/94‑1 (MQ=60)

                                           TTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCA                                                  >  SRR4302533.436628/1‑94 (MQ=60)

                                             CGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACC                                                >  SRR4302533.4610788/1‑94 (MQ=60)

                                             CGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGACGTCTTCGCCCAGACCGGTGGAGTCGTGGGgggaaaacatccac                                                >  SRR4302533.2213330/1‑80 (MQ=60)

                                               GGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTG                                              <  SRR4302533.2628717/94‑1 (MQ=60)

                                                GGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGC                                             >  SRR4302533.3179764/1‑94 (MQ=60)

                                                GGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGA                                             <  SRR4302533.1192294/94‑1 (MQ=60)

                                                 GTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGAC                                            >  SRR4302533.32843/1‑94 (MQ=60)

                                                 GTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGAC                                            <  SRR4302533.1752650/94‑1 (MQ=60)

                                                 GTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGAC                                            <  SRR4302533.535250/94‑1 (MQ=60)

                                                  TTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACG                                           <  SRR4302533.5866794/94‑1 (MQ=60)

                                                  TTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACG                                           <  SRR4302533.4020969/94‑1 (MQ=60)

                                                      GCGCAGAGAAGCGTCCTGCACAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGT                                       <  SRR4302533.2042607/94‑1 (MQ=60)

                                                      GCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGT                                       <  SRR4302533.3654575/94‑1 (MQ=60)

                                                       CGCAGAGAAGCGCCCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTT                                      <  SRR4302533.1534968/94‑1 (MQ=60)

                                                       CGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTT                                      <  SRR4302533.2473863/94‑1 (MQ=60)

                                                        GCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTT                                     <  SRR4302533.3574061/94‑1 (MQ=60)

                                                         CAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTC                                    >  SRR4302533.758702/1‑94 (MQ=60)

                                                         CAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTC                                    <  SRR4302533.4402554/94‑1 (MQ=60)

                                                         CAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTC                                    <  SRR4302533.247193/94‑1 (MQ=60)

                                                          AGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCA                                   >  SRR4302533.3662780/1‑94 (MQ=60)

                                                           GAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCAT                                  <  SRR4302533.1243827/94‑1 (MQ=60)

                                                            AGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATC                                 >  SRR4302533.5901951/1‑94 (MQ=60)

                                                            AGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATC                                 >  SRR4302533.1553022/1‑94 (MQ=60)

                                                              AAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAG                               >  SRR4302533.1563812/1‑94 (MQ=60)

                                                               AGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGC                              <  SRR4302533.1739909/94‑1 (MQ=60)

                                                               AGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGC                              >  SRR4302533.3344911/1‑94 (MQ=60)

                                                                 CGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGC                            <  SRR4302533.729891/94‑1 (MQ=60)

                                                                 CGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGC                            >  SRR4302533.3253885/1‑94 (MQ=60)

                                                                  GACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCA                           >  SRR4302533.3783710/1‑94 (MQ=60)

                                                                  GACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCA                           <  SRR4302533.3390578/94‑1 (MQ=60)

                                                                  GACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCA                           >  SRR4302533.5600367/1‑94 (MQ=60)

                                                                  GACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCA                           >  SRR4302533.2482552/1‑94 (MQ=60)

                                                                  GACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGCTTCATCAGCGCA                           >  SRR4302533.178135/1‑94 (MQ=60)

                                                                   ACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAG                          <  SRR4302533.4710843/94‑1 (MQ=60)

                                                                   ACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAG                          >  SRR4302533.125225/1‑94 (MQ=60)

                                                                   ACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAG                          >  SRR4302533.1672781/1‑94 (MQ=60)

                                                                    CCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGC                         <  SRR4302533.780572/94‑1 (MQ=60)

                                                                    CCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGC                         >  SRR4302533.2821342/1‑94 (MQ=60)

                                                                     CTGCTCAACCGGCTGGTGAGTCGGGACGTCTTCGCCCAGACCGATGGAGTCGTGGGGGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGTC                        >  SRR4302533.5510867/1‑94 (MQ=60)

                                                                      TGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCA                       >  SRR4302533.3686578/1‑94 (MQ=60)

                                                                       GCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCAT                      >  SRR4302533.1815688/1‑94 (MQ=60)

                                                                          CAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACG                   <  SRR4302533.6223079/94‑1 (MQ=60)

                                                                          CAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACG                   <  SRR4302533.885669/94‑1 (MQ=60)

                                                                            ACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTA                 <  SRR4302533.3420011/94‑1 (MQ=60)

                                                                             CCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTAC                <  SRR4302533.2558970/94‑1 (MQ=60)

                                                                             CCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTAC                <  SRR4302533.5953893/94‑1 (MQ=60)

                                                                             CCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTAC                <  SRR4302533.4748626/94‑1 (MQ=60)

                                                                             CCGGCTGGTGAGTCGGGCCGCCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTAC                <  SRR4302533.727714/94‑1 (MQ=60)

                                                                              CGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCCCCTGACGCTGTTTCATCAGCGCAGCCATACGTACG               <  SRR4302533.4368676/94‑1 (MQ=60)

                                                                              CGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACG               <  SRR4302533.816069/94‑1 (MQ=60)

                                                                              CGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACG               <  SRR4302533.5913206/94‑1 (MQ=60)

                                                                              CGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACG               <  SRR4302533.5297594/94‑1 (MQ=60)

                                                                              CGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACG               <  SRR4302533.735907/94‑1 (MQ=60)

                                                                                 CTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCG            >  SRR4302533.1099324/1‑94 (MQ=60)

                                                                                 CTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCG            <  SRR4302533.4577538/94‑1 (MQ=60)

                                                                                  TGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGT           <  SRR4302533.3694229/94‑1 (MQ=60)

                                                                                  TGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGT           <  SRR4302533.3457464/94‑1 (MQ=60)

                                                                                  TGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGT           >  SRR4302533.2621365/1‑94 (MQ=60)

                                                                                    GTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTA         <  SRR4302533.815232/94‑1 (MQ=60)

                                                                                    GTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGGTA         >  SRR4302533.5465597/1‑94 (MQ=60)

                                                                                      GAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACG       <  SRR4302533.5380642/94‑1 (MQ=60)

                                                                                      GAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACG       <  SRR4302533.1159537/94‑1 (MQ=60)

                                                                                       AGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGT      <  SRR4302533.504894/94‑1 (MQ=60)

                                                                                        GTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGTG     <  SRR4302533.1211355/94‑1 (MQ=60)

                                                                                          CGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGTGCG   >  SRR4302533.5919022/1‑94 (MQ=60)

                                                                                          CGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGTGCG   >  SRR4302533.4673251/1‑94 (MQ=60)

                                                                                          CGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGTGCG   >  SRR4302533.2820104/1‑94 (MQ=60)

                                                                                           GGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGTGCGT  <  SRR4302533.5627584/94‑1 (MQ=60)

                                                                                           GGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGTGCGT  >  SRR4302533.3085709/1‑94 (MQ=60)

GACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGCCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGTGCGT  >  NC_000913/3078152‑3078336

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`