Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,099,058	(C)_7→8	coding (585/720 nt)	yggN ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,099,051	1	.	C	96.2%	555.0 / 13.7	182	coding (592/720 nt)	yggN	hypothetical protein
Reads supporting (aligned to +/- strand): ref base . (4/3); new base C (103/72); total (107/75)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.53e-01

Only 100 of 216 total aligned reads displayed.

TTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑G‑‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCT  >  NC_000913/3098959‑3099137

 tCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑G‑‑c                                                                                        >  2:4545283/1‑93 (MQ=255)

   cAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑G‑‑ccc                                                                                      >  1:1627536/1‑93 (MQ=255)

   cAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑G‑‑ccc                                                                                      >  2:2838126/1‑93 (MQ=255)

   cAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑G‑‑ccc                                                                                      <  2:401375/93‑1 (MQ=255)

     gagTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑G‑‑ccc‑cc                                                                                   >  2:6275723/1‑93 (MQ=255)

       gTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑G‑‑ccc‑cccc                                                                                 <  1:1923666/93‑1 (MQ=255)

        tCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑G‑‑ccc‑ccccc                                                                                <  1:1232804/93‑2 (MQ=255)

        tCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑G‑‑ccc‑ccccc                                                                                >  1:4734418/1‑92 (MQ=255)

         cgcaACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGATTTGTCCACTCGGTTTGGATTGAGGATTGCC‑G‑‑ccc‑ccccca                                                                               <  2:1066289/91‑3 (MQ=255)

         cacaACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑G‑‑cct‑ccccca                                                                               <  2:2806743/93‑3 (MQ=255)

         cacaACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑G‑‑ccc‑ccccca                                                                               >  2:1100745/1‑91 (MQ=255)

          acaACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑G‑‑ccc‑cccccag                                                                              <  1:4988098/93‑4 (MQ=255)

          acaACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑G‑‑ccc‑cccccag                                                                              <  2:1769503/93‑4 (MQ=255)

          acaACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑G‑‑ccc‑cccccag                                                                              <  2:44551/93‑4 (MQ=255)

          acaACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑G‑‑ccc‑cccccag                                                                              <  2:4582550/93‑4 (MQ=255)

          acaACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑G‑‑ccc‑cccccag                                                                              <  2:5632872/93‑4 (MQ=255)

           caACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑G‑‑ccc‑cccccagg                                                                             >  2:5547648/1‑89 (MQ=255)

             aCGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTTCA‑G‑‑CCCACCACAGGCt                                                                            >  2:5518210/1‑93 (MQ=255)

              cgcgGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCtt                                                                           >  1:5752762/1‑93 (MQ=255)

               gcgGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTc                                                                          >  2:2443424/1‑93 (MQ=255)

                cgGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTcc                                                                         <  2:2267846/93‑1 (MQ=255)

                 ggCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGGTTccc                                                                        >  2:2670827/1‑93 (MQ=255)

                    tACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGc                                                                     >  2:1402821/1‑93 (MQ=255)

                    tACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGc                                                                     >  1:3937240/1‑93 (MQ=255)

                    tACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGc                                                                     >  1:3807433/1‑93 (MQ=255)

                     aCAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGca                                                                    <  2:3719761/93‑1 (MQ=255)

                     aCAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGca                                                                    >  1:993865/1‑93 (MQ=255)

                       aaaCATCTTTGCGAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGcaca                                                                  <  2:2451015/93‑1 (MQ=255)

                         aCATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACAtt                                                                >  1:4006408/1‑93 (MQ=255)

                         aCATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACAtt                                                                <  2:4080371/93‑1 (MQ=255)

                          cATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCCGCACATTc                                                               >  2:1659979/1‑93 (MQ=255)

                          cATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTc                                                               <  2:3247520/93‑1 (MQ=255)

                          cATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTc                                                               <  2:4173141/93‑1 (MQ=255)

                          cATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTc                                                               >  1:107327/1‑93 (MQ=255)

                           aTCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCt                                                              >  2:5927377/1‑93 (MQ=255)

                            tCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCTg                                                             <  1:5075174/93‑1 (MQ=255)

                            tCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCTg                                                             >  2:5753481/1‑93 (MQ=255)

                              tttGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTa                                                           >  2:4051163/1‑93 (MQ=255)

                               ttGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTaa                                                          >  2:1465896/1‑93 (MQ=255)

                               ttGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTaa                                                          <  2:507774/93‑1 (MQ=255)

                               ttGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑G‑‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTAAt                                                         <  1:2073774/93‑1 (MQ=255)

                                tGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTAAt                                                         <  2:1293806/93‑1 (MQ=255)

                                 gCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTAATg                                                        >  1:4832022/1‑93 (MQ=255)

                                  ccAAATTCCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTAAGGATTGCACC‑‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTAATgg                                                       <  1:4194735/93‑1 (MQ=255)

                                  ccAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTAATgg                                                       <  1:5878293/93‑1 (MQ=255)

                                  ccAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTAATgg                                                       >  1:2795970/1‑93 (MQ=255)

                                   cAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTAATggg                                                      <  1:421787/93‑1 (MQ=255)

                                    aaaCTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTAATGGGt                                                     <  1:903309/93‑1 (MQ=255)

                                        tgctgGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTAATGGGTTAcc                                                 <  1:4030872/93‑1 (MQ=255)

                                        tgctgGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTAATGGGTTAcc                                                 <  1:4305481/93‑1 (MQ=255)

                                        tgctgGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTAATGGGTTAcc                                                 <  1:5262613/93‑1 (MQ=255)

                                         gctgGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTAATGGGTTAccg                                                >  2:1310126/1‑93 (MQ=255)

                                           tgGAAATCTTTTTCCTGCTTTTTCCACTCGGGTTGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTAATGGGTTAccgcc                                              >  2:2754726/1‑93 (MQ=255)

                                             gAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACcgccgc                                            >  1:3071923/1‑93 (MQ=255)

                                               aaTCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCtt                                          >  1:2001854/1‑93 (MQ=255)

                                                 tCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTAATGGGTTAGCGCCGCtttt                                        >  1:3316681/1‑93 (MQ=255)

                                                  cTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTc                                       >  1:5396236/1‑93 (MQ=255)

                                                  cTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTc                                       >  2:5127035/1‑93 (MQ=255)

                                                   tttttCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCa                                      <  1:1744899/93‑1 (MQ=255)

                                                       tCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCAc                                  >  2:3396288/1‑93 (MQ=255)

                                                       tCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCAc                                  <  2:29346/93‑1 (MQ=255)

                                                         cTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCg                                >  2:1341082/1‑93 (MQ=255)

                                                          tGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGc                               >  1:5802587/1‑93 (MQ=255)

                                                           gCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCt                              >  1:5639886/1‑93 (MQ=255)

                                                           gCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGATTTTTAGCACCGCt                              >  2:504122/1‑93 (MQ=255)

                                                           gCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑G‑‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCtt                             >  1:3627572/1‑93 (MQ=255)

                                                             tttttCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCttt                            <  2:1032068/93‑1 (MQ=255)

                                                             tttttCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCttt                            <  2:2257120/93‑1 (MQ=255)

                                                              ttttCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTc                           <  2:674099/93‑1 (MQ=255)

                                                              ttttCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTc                           <  2:4765764/93‑1 (MQ=255)

                                                                 tCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTcgcg                        >  2:212531/1‑93 (MQ=255)

                                                                 tCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTcgcg                        >  2:1187116/1‑93 (MQ=255)

                                                                  ccACTCGGTTTGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTCATGGGATACCACCGCTTTTCAGCACCGCTTTCgcgc                       >  2:2842641/1‑93 (MQ=255)

                                                                  ccACTCGGTTTGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCgcgc                       <  1:2633332/93‑1 (MQ=255)

                                                                    aCTCGGTTTGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGccc                     <  2:6254969/93‑1 (MQ=255)

                                                                     cTCGGTTTGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCa                    >  1:2189444/1‑93 (MQ=255)

                                                                     cTCGGTTTGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCa                    >  1:2962712/1‑93 (MQ=255)

                                                                      tCGGTTTGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCAt                   >  1:4922389/1‑93 (MQ=255)

                                                                       cGGTTTGGGTTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCAtt                  >  2:6135320/1‑93 (MQ=255)

                                                                         gTTTGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTc                >  1:2542765/1‑93 (MQ=255)

                                                                          tttGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCa               >  2:5165233/1‑93 (MQ=255)

                                                                          tttGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCa               >  2:4366104/1‑93 (MQ=255)

                                                                          tttGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCa               <  1:3233096/93‑1 (MQ=255)

                                                                          tttGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCa               <  1:1007498/93‑1 (MQ=255)

                                                                            tGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCAtt             <  2:3692734/93‑1 (MQ=255)

                                                                            tGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCAtt             <  2:2640434/93‑1 (MQ=255)

                                                                            tGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCAtt             <  2:235299/93‑1 (MQ=255)

                                                                             ggATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTa            >  2:958024/1‑93 (MQ=255)

                                                                              gATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTaa           <  1:197119/93‑1 (MQ=255)

                                                                              gATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTaa           <  1:1350529/93‑1 (MQ=255)

                                                                               aTTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAAt          >  1:1554160/1‑93 (MQ=255)

                                                                               aTTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTACATTAAt          <  2:5985374/93‑1 (MQ=255)

                                                                                  gAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCt       >  1:2999916/1‑93 (MQ=255)

                                                                                  gAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCt       <  2:69618/93‑1 (MQ=255)

                                                                                   aGGATTGCACC‑‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTg      <  1:3365442/93‑1 (MQ=255)

                                                                                    ggATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGt     >  1:1422330/1‑93 (MQ=255)

                                                                                     gATTGCA‑GCCCCC‑CCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGt     >  2:4906926/1‑93 (MQ=255)

                                                                                     gATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTc    >  1:6232673/1‑93 (MQ=255)

                                                                                       ttGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCt  >  1:2489124/1‑93 (MQ=255)

                                                                                       ttGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCt  <  1:2919794/93‑1 (MQ=255)

TTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑G‑‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCT  >  NC_000913/3098959‑3099137

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑G‑CCCC‑CCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCC  >  NC_000913/3098966‑3099151

GTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑G‑CCCC‑CCCC                                                                                              <  SRR4302533.1935403/94‑1 (MQ=60)

GTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑G‑CCCC‑CCCC                                                                                              >  SRR4302533.4763119/1‑94 (MQ=60)

 TCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑G‑CCCC‑CCCca                                                                                             <  SRR4302533.1240181/94‑3 (MQ=60)

 TCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑G‑CCCC‑CCCca                                                                                             <  SRR4302533.4606821/94‑3 (MQ=60)

   ACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GCCCCC‑CCCAGG                                                                                            <  SRR4302533.5018343/94‑1 (MQ=60)

    CAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GCCCCC‑CCCAGGC                                                                                           >  SRR4302533.396762/1‑94 (MQ=60)

      ACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GCCCCC‑CCCAGGCTT                                                                                         >  SRR4302533.5787471/1‑94 (MQ=60)

       CGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GCCCCC‑CCCAGGCTTC                                                                                        >  SRR4302533.3270111/1‑94 (MQ=60)

        GCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GCCCCC‑CCCAGGCTTCC                                                                                       >  SRR4302533.6158882/1‑94 (MQ=60)

          GGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GCCCCC‑CCCAGGCTTCCCA                                                                                     <  SRR4302533.762071/94‑1 (MQ=60)

            CTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GCCCCC‑CCCAGGCTTCCCAGC                                                                                   >  SRR4302533.3830726/1‑94 (MQ=60)

            CTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GCCCCC‑CCCAGGCTTCCCAGC                                                                                   >  SRR4302533.3961275/1‑94 (MQ=60)

             TACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GCCCCC‑CCCAGGCTTCCCAGCA                                                                                  >  SRR4302533.999674/1‑94 (MQ=60)

               CAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GCCCCC‑CCCAGGCTTCCCAGCACA                                                                                >  SRR4302533.4854865/1‑94 (MQ=60)

                 AACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GCCCCC‑CCCAGGCTTCCCAGCACATT                                                                              >  SRR4302533.4030801/1‑94 (MQ=60)

                  ACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GCCCCC‑CCCAGGCTTCCCAGCACATTC                                                                             >  SRR4302533.108003/1‑94 (MQ=60)

                    ATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GCCCCC‑CCCAGGCTTCCCAGCACATTCTG                                                                           <  SRR4302533.966153/94‑1 (MQ=60)

                     TCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GCCCCC‑CCCAGGCTTCCCAGCACATTCTGT                                                                          <  SRR4302533.5105853/94‑1 (MQ=60)

                       TTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GCCCCC‑CCCAGGCTTCCCAGCACATTCTGTAA                                                                        <  SRR4302533.1519931/94‑1 (MQ=60)

                       TTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GCCCCC‑CCCAGGCTTCCCAGCACATTCTGTAA                                                                        >  SRR4302533.2043033/1‑94 (MQ=60)

                        TTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑G‑CCCC‑CCCAGGCTTCCCAGCACATTCTGTAATG                                                                      <  SRR4302533.2086545/94‑1 (MQ=60)

                         TGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GCCCCC‑CCCAGGCTTCCCAGCACATTCTGTAATG                                                                      <  SRR4302533.2568917/94‑1 (MQ=60)

                         TGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GCCCCC‑CCCAGGCTTCCCAGCACATTCTGTAATG                                                                      >  SRR4302533.4861309/1‑94 (MQ=60)

                         TGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GCCCCC‑CCCAGGCTTCCCAGCACATTCTGTAATG                                                                      >  SRR4302533.1988962/1‑94 (MQ=60)

                          GCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GCCCCC‑CCCAGGCTTCCCAGCACATTCTGTAATGG                                                                     >  SRR4302533.2813165/1‑94 (MQ=60)

                           CCAAATTCCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTAAGGATTGCACC‑CCCC‑CCCAGGCTTCCCAGCACATTCTGTAATGGG                                                                    <  SRR4302533.4220126/94‑1 (MQ=60)

                           CCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GCCCCC‑CCCAGGCTTCCCAGCACATTCTGTAATGGG                                                                    <  SRR4302533.5913824/94‑1 (MQ=60)

                           CCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GCCCCC‑CCCAGGCTTCCCAGCACATTCTGTAATGGG                                                                    <  SRR4302533.5752056/94‑1 (MQ=60)

                            CAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GCCCCC‑CCCAGGCTTCCCAGCACATTCTGTAATGGGT                                                                   >  SRR4302533.1029212/1‑94 (MQ=60)

                            CAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GCCCCC‑CCCAGGCTTCCCAGCACATTCTGTAATGGGT                                                                   <  SRR4302533.424310/94‑1 (MQ=60)

                             AAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GCCCCC‑CCCAGGCTTCCCAGCACATTCTGTAATGGGTT                                                                  <  SRR4302533.908606/94‑1 (MQ=60)

                             AAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GCCCCC‑CCCAGGCTTCCCAGCACATTCTGTAATGGGTT                                                                  <  SRR4302533.5578198/94‑1 (MQ=60)

                              AACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GCCCCC‑CCCAGGCTTCCCAGCACATTCTGTAATGGGTTA                                                                 <  SRR4302533.3347252/94‑1 (MQ=60)

                                CTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GCCCCC‑CCCAGGCTTCCCAGCACATTCTGTAATGGGTTACC                                                               <  SRR4302533.651773/94‑1 (MQ=60)

                                CTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GCCCCC‑CCCAGGCTTCCCAGCACATTCTGTAATGGGTTACC                                                               >  SRR4302533.1607647/1‑94 (MQ=60)

                                 TGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GCCCCC‑CCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCG                                                              <  SRR4302533.4055407/94‑1 (MQ=60)

                                 TGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GCCCCC‑CCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCG                                                              <  SRR4302533.4331387/94‑1 (MQ=60)

                                 TGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GCCCCC‑CCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCG                                                              <  SRR4302533.5294202/94‑1 (MQ=60)

                                   CTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GCCCCC‑CCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCC                                                            >  SRR4302533.185612/1‑94 (MQ=60)

                                    TGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GCCCCC‑CCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCG                                                           >  SRR4302533.1736557/1‑94 (MQ=60)

                                    TGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑G‑CCCC‑CCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGC                                                          >  SRR4302533.4299035/1‑94 (MQ=60)

                                     GGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GCCCCC‑CCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGC                                                          >  SRR4302533.3090783/1‑94 (MQ=60)

                                      GAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑G‑CCCCACCCAGGCCTCCCAGCACATTCTGCAATGGGTTACCGCCGCT                                                         >  SRR4302533.1406566/1‑94 (MQ=60)

                                       AAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GCCCCC‑CCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTT                                                        >  SRR4302533.271318/1‑94 (MQ=60)

                                       AAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GCCCCC‑CCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTT                                                        >  SRR4302533.4034541/1‑94 (MQ=60)

                                       AAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GCCCCC‑CCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTT                                                        >  SRR4302533.2014179/1‑94 (MQ=60)

                                       AAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GCCCCC‑CCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTT                                                        >  SRR4302533.1240238/1‑94 (MQ=60)

                                        AATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGG‑CCCC‑CCCAGGCTTCCCAGCACATTCTGTTATGGGTTACCGCTGCTTT                                                       >  SRR4302533.2861946/1‑94 (MQ=60)

                                         ATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GCCCCC‑CCCAGGCTTCCCAGCACATTCTGTAATGGGTTAGCGCCGCTTTT                                                      >  SRR4302533.3337164/1‑94 (MQ=60)

                                         ATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GCCCCC‑CCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTT                                                      >  SRR4302533.4932916/1‑94 (MQ=60)

                                          TCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GCCCCC‑CCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTC                                                     >  SRR4302533.5428661/1‑94 (MQ=60)

                                           CTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GCCCCC‑CCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCA                                                    <  SRR4302533.3125547/94‑1 (MQ=60)

                                           CTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GCCCCC‑CCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCA                                                    <  SRR4302533.2099239/94‑1 (MQ=60)

                                           CTTTTGCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GCCCCC‑CCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCA                                                    <  SRR4302533.2440809/94‑1 (MQ=60)

                                            TTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GCCCCC‑CCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAG                                                   <  SRR4302533.1755511/94‑1 (MQ=60)

                                              TTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GCCCCC‑CCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCC                                                 >  SRR4302533.1106462/1‑94 (MQ=60)

                                              TTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GCCCCC‑CCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCA                                                 <  SRR4302533.1951113/94‑1 (MQ=60)

                                               TTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GCCCCC‑CCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCAC                                                >  SRR4302533.5911361/1‑94 (MQ=60)

                                                TCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GCCCCC‑CCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACC                                               <  SRR4302533.3007757/94‑1 (MQ=60)

                                                  CTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GCCCCC‑CCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGC                                             >  SRR4302533.5837637/1‑94 (MQ=60)

                                                   TGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GCCCCC‑CCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCT                                            >  SRR4302533.5673978/1‑94 (MQ=60)

                                                   TGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GCCCCC‑CCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCT                                            >  SRR4302533.5364998/1‑94 (MQ=60)

                                                   TGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑G‑CCCC‑CCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTT                                           >  SRR4302533.3649893/1‑94 (MQ=60)

                                                    GCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GCCCCC‑CCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTT                                           >  SRR4302533.2953004/1‑94 (MQ=60)

                                                     CTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GCCCCC‑CCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTT                                          >  SRR4302533.5825949/1‑94 (MQ=60)

                                                     CTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GCCCCC‑CCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTT                                          >  SRR4302533.500052/1‑94 (MQ=60)

                                                      TTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GCCCCC‑CCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTC                                         >  SRR4302533.3715420/1‑94 (MQ=60)

                                                           CCACTCGGTTTGGATTGAGGATTGCA‑GCCCCC‑CCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCC                                    <  SRR4302533.2649515/94‑1 (MQ=60)

                                                             ACTCGGTTTGGATTGAGGATTGCA‑GCCCCC‑CCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCA                                  <  SRR4302533.5364601/94‑1 (MQ=60)

                                                             ACTCGGTTTGGATTGAGGATTGCA‑GCCCCC‑CCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCA                                  >  SRR4302533.2980824/1‑94 (MQ=60)

                                                             ACTCGGTTTGGATTGAGGATTGCA‑GCCCCC‑CCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCA                                  >  SRR4302533.2202932/1‑94 (MQ=60)

                                                              CTCGGTTTGGATTGAGGATTGCA‑GCCCCC‑CCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCAT                                 >  SRR4302533.4952207/1‑94 (MQ=60)

                                                               TCGGTTTGGATTGAGGATTGCA‑GCCCCC‑CCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATT                                <  SRR4302533.4447475/94‑1 (MQ=60)

                                                                CGGTTTGGATTGAGGATTGCA‑GCCCCC‑CCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTT                               <  SRR4302533.3051411/94‑1 (MQ=60)

                                                                CGGTTTGGATTGAGGATTGCA‑GCCCCC‑CCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTT                               <  SRR4302533.3011023/94‑1 (MQ=60)

                                                                 GGTTTGGATTGAGGATTGCA‑GCCCCC‑CCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTC                              >  SRR4302533.2558413/1‑94 (MQ=60)

                                                                  GTTTGGATTGAGGATTGCA‑GCCCCC‑CCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCA                             >  SRR4302533.4169697/1‑94 (MQ=60)

                                                                  GTTTGGATTGAGGATTGCA‑GCCCCC‑CCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCA                             >  SRR4302533.2552101/1‑94 (MQ=60)

                                                                   TTTGGATTGAGGATTGCA‑GCCCCC‑CCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCAT                            <  SRR4302533.1013392/94‑1 (MQ=60)

                                                                   TTTGGATTGAGGATTGCA‑GCCCCC‑CCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCAT                            <  SRR4302533.3253019/94‑1 (MQ=60)

                                                                     TGGATTGAGGATTGCA‑GCCCCC‑CCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTA                          <  SRR4302533.3716861/94‑1 (MQ=60)

                                                                     TGGATTGAGGATTGCA‑GCCCCC‑CCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTA                          >  SRR4302533.525014/1‑94 (MQ=60)

                                                                       GATTGAGGATTGCA‑GCCCCC‑CCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAAT                        <  SRR4302533.198362/94‑1 (MQ=60)

                                                                       GATTGAGGATTGCA‑GCCCCC‑CCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAAT                        <  SRR4302533.3962193/94‑1 (MQ=60)

                                                                       GATTGAGGATTGCA‑GCCCCC‑CCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAAT                        <  SRR4302533.1358651/94‑1 (MQ=60)

                                                                       GATTGAGGATTGCA‑GCCCCC‑CCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAAT                        >  SRR4302533.1563517/1‑94 (MQ=60)

                                                                          TGAGGATTGCA‑GCCCCC‑CCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCT                     >  SRR4302533.3018263/1‑94 (MQ=60)

                                                                          TGAGGATTGCA‑GCCCCC‑CCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCT                     >  SRR4302533.3936051/1‑94 (MQ=60)

                                                                            AGGATTGCACC‑CCCC‑CCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGT                   <  SRR4302533.3386231/94‑1 (MQ=60)

                                                                            AGGATTGCA‑GCCCCC‑CCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGT                   >  SRR4302533.1430928/1‑94 (MQ=60)

                                                                             GGATTGCA‑GCCCCC‑CCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTC                  >  SRR4302533.6269996/1‑94 (MQ=60)

                                                                               ATTGCA‑GCCCCC‑CCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCT                >  SRR4302533.2504453/1‑94 (MQ=60)

                                                                               ATTGCA‑GCCCCC‑CCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCT                <  SRR4302533.2214618/94‑1 (MQ=60)

                                                                                gttgcaccCCCC‑CCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCT                <  SRR4302533.3959936/86‑1 (MQ=60)

                                                                                TTGCA‑GCCCCC‑CCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTG               <  SRR4302533.2937684/94‑1 (MQ=60)

                                                                                     agcCCCC‑CCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAA           <  SRR4302533.110812/91‑1 (MQ=60)

                                                                                      C‑CCCC‑CCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAAT         <  SRR4302533.752854/94‑1 (MQ=60)

                                                                                        CCCC‑CCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATT        <  SRR4302533.3455919/94‑1 (MQ=60)

                                                                                        CCCC‑CCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATT        <  SRR4302533.5376693/94‑1 (MQ=60)

                                                                                         CCC‑CCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTC       >  SRR4302533.1912595/1‑94 (MQ=60)

                                                                                         CCC‑CCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTC       >  SRR4302533.2207377/1‑94 (MQ=60)

                                                                                         CCC‑CCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTC       >  SRR4302533.3462301/1‑94 (MQ=60)

                                                                                          CC‑CCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCC      <  SRR4302533.1605468/94‑1 (MQ=60)

                                                                                           C‑CCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCA     >  SRR4302533.491231/1‑94 (MQ=60)

                                                                                           C‑CCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTGTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCA     >  SRR4302533.3401664/1‑94 (MQ=60)

                                                                                             CCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGTCCATTTCATTAATGCTGTCCTGTAAAATTCCAC    >  SRR4302533.2998840/1‑94 (MQ=60)

                                                                                             CCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCAC    <  SRR4302533.2118695/94‑1 (MQ=60)

                                                                                              CCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACC   <  SRR4302533.4513897/94‑1 (MQ=60)

                                                                                              CCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACC   >  SRR4302533.1611471/1‑94 (MQ=60)

                                                                                               CAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCC  >  SRR4302533.3237262/1‑94 (MQ=60)

GTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑G‑CCCC‑CCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCC  >  NC_000913/3098966‑3099151

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`