Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A0 F0 I1 R1 245 579.6 28867798 97.7% 28203838 96.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,099,661 (A)5→6 intergenic (‑19/+164) yggN ← / ← b2959 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,099,6561.A99.3% 2318.4 / 3.6 578intergenic (‑14/+169)yggN/b2959hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (2/2);  new base A (298/275);  total (300/278)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.19e-01

Only 100 of 642 total aligned reads displayed.

GCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAG‑AAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTA  >  NC_000913/3099561‑3099746
                                                                                                |                                                                                           
 cgTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAG‑a                                                                                            >  1:12376972/1‑96 (MQ=255)
 cgTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAG‑a                                                                                            <  1:5766354/96‑1 (MQ=255)
  gTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAG‑aa                                                                                           >  2:3333888/1‑96 (MQ=255)
  gTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAG‑aa                                                                                           >  1:5917666/1‑96 (MQ=255)
  gTGAAGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCCTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAG‑aa                                                                                           >  1:12637589/1‑96 (MQ=255)
  atGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAG‑aa                                                                                           >  1:11335423/2‑96 (MQ=255)
   tGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAG‑aaa                                                                                          <  2:3833324/96‑1 (MQ=255)
     aCGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAG‑aaaaa                                                                                        <  2:6234459/96‑1 (MQ=255)
      cGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAG‑aaaaaa                                                                                       <  2:2358350/96‑2 (MQ=255)
       gCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAG‑aaaaaat                                                                                      >  2:7251182/1‑94 (MQ=255)
        cTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAG‑aaaaaatc                                                                                     >  2:4810519/1‑93 (MQ=255)
         tGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAG‑aaaaaatca                                                                                    >  2:9099743/1‑92 (MQ=255)
         tGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAG‑aaaaaatca                                                                                    >  1:8370679/1‑92 (MQ=255)
             cTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGcc                                                                                <  2:924561/96‑1 (MQ=255)
             cTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGcc                                                                                >  2:1391705/1‑96 (MQ=255)
              tGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCa                                                                               <  2:8341731/96‑1 (MQ=255)
                   gTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGAATCATAGTCTTCCCTCAAGAAAAAA‑TAAGGCCAGCAtt                                                                          >  1:13546081/1‑96 (MQ=255)
                    tCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCAttt                                                                         <  1:3361142/96‑1 (MQ=255)
                     cGGCGTGAGCGGTCATTGCCGTCCCTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTg                                                                        <  2:12630028/96‑1 (MQ=255)
                        cGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTg                                                                     <  1:5066528/96‑1 (MQ=255)
                        cGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTg                                                                     >  2:11280015/1‑96 (MQ=255)
                        cGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTg                                                                     >  2:3972679/1‑96 (MQ=255)
                         gTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTgg                                                                    >  2:10678251/1‑96 (MQ=255)
                         gTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTgg                                                                    >  1:5298558/1‑96 (MQ=255)
                          tGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGc                                                                   >  2:3224809/1‑96 (MQ=255)
                          tGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGc                                                                   <  2:5403207/96‑1 (MQ=255)
                           gAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGcc                                                                  <  1:3229344/96‑1 (MQ=255)
                           gAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGcc                                                                  <  2:13054344/96‑1 (MQ=255)
                           gAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGcc                                                                  >  2:1095096/1‑96 (MQ=255)
                            aGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGccc                                                                 <  2:3731154/96‑1 (MQ=255)
                             gCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGcccc                                                                <  2:793024/96‑1 (MQ=255)
                               ggTCATTGCCGTCCCTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCac                                                              <  1:13811998/96‑2 (MQ=255)
                               ggTCATTGCCGTCACTGCAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAg                                                              <  1:8947673/96‑1 (MQ=255)
                               ggTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAg                                                              <  2:11895158/96‑1 (MQ=255)
                                 tCATTGCCGTCACTGAAAGAGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGAt                                                            >  2:12361090/1‑96 (MQ=255)
                                  cATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGAtt                                                           <  2:13791054/96‑1 (MQ=255)
                                  cATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGAtt                                                           >  2:9575592/1‑96 (MQ=255)
                                  cATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGAtt                                                           >  1:4814949/1‑96 (MQ=255)
                                   aTTGCCGTCACTTAAAGGTCTGCCGCCAGCAGAATTTTTCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTg                                                          <  1:1779477/96‑1 (MQ=255)
                                   aTTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTg                                                          >  2:5582016/1‑96 (MQ=255)
                                       ccGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATaa                                                      <  2:11761495/96‑1 (MQ=255)
                                       ccGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAG‑AAAAAGTCAGGCCAGCATTTGCTGGCCCCAGATTGATaa                                                      <  2:10259552/96‑1 (MQ=255)
                                        catCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAAc                                                     >  1:7352257/3‑96 (MQ=255)
                                        cGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAAc                                                     >  2:6974683/1‑96 (MQ=255)
                                          tCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑GCAGGCCAGCATTTGCTGGCCCCAGATTGATAACaa                                                   <  2:12087708/96‑1 (MQ=255)
                                              tGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCATCAAGAAAAAA‑TCAGGCCAGCATTTGATGGCCCCAGATTGATAACAAAGTa                                               >  1:13023832/1‑96 (MQ=255)
                                                     gctgcCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGttt                                        <  1:4020130/96‑1 (MQ=255)
                                                     gctgcCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGttt                                        <  2:135872/96‑1 (MQ=255)
                                                     gctgcCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGttt                                        <  1:10425482/96‑1 (MQ=255)
                                                      ctgcCGCCAGCATCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGtttt                                       <  1:8731767/96‑1 (MQ=255)
                                                      ctgcCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGtttt                                       <  2:5144870/96‑1 (MQ=255)
                                                       tgcCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTc                                      <  2:11072975/96‑1 (MQ=255)
                                                       tgcCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTc                                      <  1:6659534/96‑1 (MQ=255)
                                                        gccgccAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTcc                                     >  1:4383301/1‑96 (MQ=255)
                                                        gccgccAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTcc                                     >  2:7645689/1‑96 (MQ=255)
                                                        gccgccAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTcc                                     <  1:11942417/96‑1 (MQ=255)
                                                          cgccAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCAt                                   <  1:10085705/96‑1 (MQ=255)
                                                          cgccAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGACAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCAt                                   >  1:14300773/1‑96 (MQ=255)
                                                            ccAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGc                                 <  2:13333807/96‑1 (MQ=255)
                                                             cagcagCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGcc                                >  2:10493464/1‑96 (MQ=255)
                                                             cagcagCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGcc                                <  2:2090357/96‑1 (MQ=255)
                                                             cagcagCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGcc                                >  2:4576517/1‑96 (MQ=255)
                                                              agcagcATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCg                               >  1:6152893/1‑96 (MQ=255)
                                                                cagcaTTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGa                             >  1:3609920/1‑96 (MQ=255)
                                                                 agcaTTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGAt                            <  1:297803/96‑1 (MQ=255)
                                                                  gcaTTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATg                           >  1:2154638/1‑96 (MQ=255)
                                                                   caTTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGTCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATgc                          <  2:8531165/96‑1 (MQ=255)
                                                                   caTTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATgc                          >  1:1509426/1‑96 (MQ=255)
                                                                   caTTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATgc                          >  2:196721/1‑96 (MQ=255)
                                                                    aTTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATgcg                         <  1:9638938/96‑1 (MQ=255)
                                                                     ttttGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGTTGGGCCCAGATTGATTACAAAGTACGCGTTTTCCATGCCGGATgcgg                        >  2:65100/1‑96 (MQ=255)
                                                                       ttGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATgcggcg                      <  2:6635424/96‑1 (MQ=255)
                                                                        tGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGt                     <  1:3763968/96‑1 (MQ=255)
                                                                        tGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGt                     <  2:1431007/96‑1 (MQ=255)
                                                                         gcgcATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTa                    <  2:8097086/96‑1 (MQ=255)
                                                                          cgcATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTaa                   >  1:7811270/1‑96 (MQ=255)
                                                                             atcntAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAaca                >  2:876535/1‑96 (MQ=255)
                                                                             atcatAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAaca                >  1:1278674/1‑96 (MQ=255)
                                                                             atcatAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAaca                <  1:4513831/96‑1 (MQ=255)
                                                                             atcatAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAaca                <  2:1625084/96‑1 (MQ=255)
                                                                             atcatAGTCTTCCCGCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTCAaca                >  1:10015250/1‑96 (MQ=38)
                                                                               catAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACAcc              >  2:29610/1‑96 (MQ=255)
                                                                                 tAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACAccct            <  1:10678251/96‑3 (MQ=255)
                                                                                 tAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCtt            <  1:7509434/96‑1 (MQ=255)
                                                                                  aGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTa           >  1:274755/1‑96 (MQ=255)
                                                                                  aGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGAGGCGGCGTAGACACCTTa           >  1:14021379/1‑96 (MQ=255)
                                                                                  aGTCTTCCCTCAAGAAAAAA‑GCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGTCGTAAACACCTTa           >  1:9395102/1‑96 (MQ=255)
                                                                                    tCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATc         <  2:4201756/96‑1 (MQ=255)
                                                                                    tCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATc         >  2:1820099/1‑96 (MQ=255)
                                                                                      ttCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATccc       <  1:11076924/96‑1 (MQ=255)
                                                                                      ttCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATccc       <  2:11547571/96‑1 (MQ=255)
                                                                                       tCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCg      <  1:8605224/96‑1 (MQ=255)
                                                                                        cccTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGc     <  1:5434172/96‑1 (MQ=255)
                                                                                        cccTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGc     <  2:5917666/96‑1 (MQ=255)
                                                                                        cccTCAAGAAAAAA‑GCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGc     <  1:2488247/96‑1 (MQ=255)
                                                                                         ccTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGcc    >  1:11702939/1‑96 (MQ=255)
                                                                                         ccTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGcc    >  1:8050739/1‑96 (MQ=255)
                                                                                          cTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCt   >  1:6387416/1‑96 (MQ=255)
                                                                                          cTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCt   <  1:6982889/96‑1 (MQ=255)
                                                                                           tCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTa  >  2:2758662/1‑96 (MQ=255)
                                                                                                |                                                                                           
GCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAG‑AAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTA  >  NC_000913/3099561‑3099746

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 385 total aligned reads displayed.
GCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAG‑A‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTCGATCGTGCATCT  >  NC_000913/3099561‑3099761
                                                                                                      |                                                                                                    
GCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAG‑A‑AAAA                                                                                                      <  SRR4302518.3540702/101‑1 (MQ=60)
GCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAG‑A‑AAAA                                                                                                      <  SRR4302518.1042028/101‑1 (MQ=60)
GCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAG‑A‑AAAA                                                                                                      >  SRR4302518.2338841/1‑101 (MQ=60)
 ggtgcCGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAG‑A‑AAAAA                                                                                                     <  SRR4302518.6039246/96‑1 (MQ=60)
 CGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAG‑A‑AANNN                                                                                                     >  SRR4302518.12960728/1‑101 (MQ=60)
  GTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAG‑A‑AAANNN                                                                                                    >  SRR4302518.10865476/1‑101 (MQ=60)
  GTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAG‑A‑AAAAat                                                                                                    >  SRR4302518.6217644/1‑99 (MQ=60)
  GTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAG‑A‑AAAAat                                                                                                    >  SRR4302518.104561/1‑99 (MQ=60)
     ACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAA‑AAAATCAG                                                                                                  <  SRR4302518.3284854/101‑1 (MQ=60)
     ACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAG‑A‑AAAAatnnn                                                                                                 >  SRR4302518.14196621/1‑96 (MQ=60)
      NNNTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAA‑AAAATCAGG                                                                                                 <  SRR4302518.8362801/101‑1 (MQ=60)
       GCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAA‑AAAATCAGGC                                                                                                >  SRR4302518.3439741/1‑101 (MQ=60)
       GCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAA‑AAAATCAGGC                                                                                                >  SRR4302518.1804429/1‑101 (MQ=60)
       GCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAA‑AAAATCAGGC                                                                                                <  SRR4302518.3628511/101‑1 (MQ=60)
       GCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAG‑A‑AAAAatcagtc                                                                                               >  SRR4302518.6977672/1‑94 (MQ=60)
       GCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGGGCTGCCGCCAGCAGCGTTTTGCGCATCATAGTCTTCCCTCAAGAA‑AAAATCAGGC                                                                                                >  SRR4302518.5335759/1‑101 (MQ=60)
        NNNCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAA‑AAAATCAGGCC                                                                                               <  SRR4302518.7840463/101‑1 (MQ=60)
           CACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAA‑AAAATCAGGCCAGC                                                                                            >  SRR4302518.4435934/1‑101 (MQ=60)
             NNNGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAA‑AAAATCAGGCCAGCAT                                                                                          <  SRR4302518.13966162/101‑1 (MQ=60)
             CTGGTAGTCGGCGGGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGGCTTCCCTCAAGAA‑AAAATCAGGCCAGCAT                                                                                          >  SRR4302518.465447/1‑101 (MQ=60)
               GGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAA‑AAAATCAGGCCAGCATTT                                                                                        <  SRR4302518.3517673/101‑1 (MQ=60)
               GGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGGGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAA‑AAAATCAGGCCAGCATTT                                                                                        >  SRR4302518.6352914/1‑101 (MQ=60)
                 TAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGGCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAA‑AAAATCAGGCCAGCATTNNN                                                                                      >  SRR4302518.8833321/1‑101 (MQ=60)
                 NNNTCGGCCTGAGCGTTCATTGCCGTCTGTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAA‑AAAATCAGGCCAGCATTTGC                                                                                      <  SRR4302518.9887431/101‑1 (MQ=60)
                 GAGTCGGCGAGAGCGGTCATTGCCGTCCGTGAATGTGCTGCCGCCAGCAGCATTTTGCGCATCTTAGTCTTCCCTAAAGAA‑AAAATCAGGCCAGCATTTGC                                                                                      <  SRR4302518.1540244/101‑1 (MQ=60)
                   GTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAA‑AAAATCAGGCCAGCATTTGNNN                                                                                    >  SRR4302518.14583925/1‑101 (MQ=60)
                    NNNGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGG                                                                                   <  SRR4302518.8578849/101‑1 (MQ=60)
                      GGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCC                                                                                 >  SRR4302518.4236444/1‑101 (MQ=60)
                      GGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCC                                                                                 >  SRR4302518.4744470/1‑101 (MQ=60)
                       GCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCC                                                                                >  SRR4302518.4611806/1‑101 (MQ=60)
                       GCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCC                                                                                <  SRR4302518.2898724/101‑1 (MQ=60)
                       ACGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGNNN                                                                                >  SRR4302518.9284629/1‑101 (MQ=60)
                         GTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCNNN                                                                              >  SRR4302518.13012053/1‑101 (MQ=60)
                         GTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCA                                                                              >  SRR4302518.2147569/1‑101 (MQ=60)
                         GTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCA                                                                              >  SRR4302518.5570521/1‑101 (MQ=60)
                          TAAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCNNN                                                                             >  SRR4302518.12295712/1‑101 (MQ=60)
                          NNNGCGGTCATTGCCGTCCCTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCAC                                                                             <  SRR4302518.14444303/101‑1 (MQ=60)
                          NNNGCGGTCATTGCCGTCACTGCAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCAG                                                                             <  SRR4302518.9380680/101‑1 (MQ=60)
                          NNNGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCAG                                                                             <  SRR4302518.8732191/101‑1 (MQ=60)
                           NNNCGGTCATTTCCGTAACTGAAAGTCCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTAGCCCCAGA                                                                            <  SRR4302518.10972332/101‑1 (MQ=60)
                           GAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCNNN                                                                            >  SRR4302518.12091003/1‑101 (MQ=60)
                            AGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGAT                                                                           <  SRR4302518.4872056/101‑1 (MQ=60)
                             GCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGAATTTTGCGCATCATAGTCTTCCCTCAAGAA‑AAAATCAGGCCAGCATTTTCTGGCaacagnnn                                                                          >  SRR4302518.13194834/1‑93 (MQ=60)
                              CTGTCATTGCCGTCACTTAAAGGTCTGCCGCCAGCAGAATTTTTCGCATCATAGTCTTCCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTG                                                                         <  SRR4302518.1859211/101‑1 (MQ=60)
                               GGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATNNN                                                                        >  SRR4302518.9740864/1‑101 (MQ=60)
                                GTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTNNN                                                                       >  SRR4302518.12191506/1‑101 (MQ=60)
                                  CATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAA                                                                     >  SRR4302518.5066953/1‑101 (MQ=60)
                                  CATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAA                                                                     >  SRR4302518.5642628/1‑101 (MQ=60)
                                  CATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGANNN                                                                     >  SRR4302518.9086821/1‑101 (MQ=60)
                                    TTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACA                                                                   >  SRR4302518.7184902/1‑101 (MQ=60)
                                    TTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACA                                                                   >  SRR4302518.6682177/1‑101 (MQ=60)
                                     TGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAA                                                                  >  SRR4302518.684712/1‑101 (MQ=60)
                                      GCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAA                                                                 <  SRR4302518.1734038/101‑1 (MQ=60)
                                       CCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAA‑AAAATCAGGCAAGCATTTGCTGGCCACAGAGTGATAACANNN                                                                >  SRR4302518.11618779/1‑101 (MQ=60)
                                        CCTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAANNN                                                               >  SRR4302518.7748477/1‑101 (MQ=60)
                                         NNNACTGAAAGTGCTCCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTA                                                              <  SRR4302518.13152168/101‑1 (MQ=60)
                                         GTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTA                                                              >  SRR4302518.4926971/1‑101 (MQ=60)
                                          TTACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGNNN                                                             >  SRR4302518.12372392/1‑101 (MQ=60)
                                          TCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTAC                                                             >  SRR4302518.1847127/1‑101 (MQ=60)
                                          TCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGNNN                                                             >  SRR4302518.10529781/1‑101 (MQ=60)
                                           tactaAAAGTGCTGCCGCCAGCAGATTTTTGCGCATCATAGTCTTCCCTCAAGAA‑AAAATCAGGCCAGAATTTGCTGGCCCCAGATTGATAACAAAGTACG                                                            <  SRR4302518.3725285/96‑1 (MQ=60)
                                           CACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAA‑AAAATCAGGACAGCATTTGCTGGCCCCAGATTGATAACAAAGTNNN                                                            >  SRR4302518.8456416/1‑101 (MQ=60)
                                           CACTGAAAGCGCTGCCGCGAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACG                                                            <  SRR4302518.6542515/101‑1 (MQ=60)
                                            ACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGC                                                           >  SRR4302518.1689134/1‑101 (MQ=60)
                                             NNNAAAGTGCTGCAGCCAGCAGCATTTTCCGCATCATAGTCTTCCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGAG                                                          <  SRR4302518.10738785/101‑1 (MQ=60)
                                             CTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACNNN                                                          >  SRR4302518.8351763/1‑101 (MQ=60)
                                             CTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACNNN                                                          >  SRR4302518.9976547/1‑101 (MQ=60)
                                              TGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGNNN                                                         >  SRR4302518.10949813/1‑101 (MQ=60)
                                              TGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGT                                                         <  SRR4302518.1324906/101‑1 (MQ=60)
                                              TGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCATCAAGAA‑AAAATCAGGCCAGCATTTGATGGCCCCAGATTGATAACAAAGTACGNNN                                                         >  SRR4302518.13626933/1‑101 (MQ=60)
                                              TGAAAGAGCTACTGCCAGCAGCATTTTGCGCAACATAGTCTTCCCTCAAGAA‑AAAATAACGCCAGCATTTGCTGGCCCCAGGTTAATAACAAAGTACGCGT                                                         >  SRR4302518.5011270/1‑101 (MQ=60)
                                               GAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTT                                                        <  SRR4302518.746592/101‑1 (MQ=60)
                                               GAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTT                                                        >  SRR4302518.5335061/1‑101 (MQ=60)
                                                NNNGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTT                                                       <  SRR4302518.10914485/101‑1 (MQ=60)
                                                GAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTT                                                       <  SRR4302518.4212905/101‑1 (MQ=60)
                                                AAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTT                                                       <  SRR4302518.6868747/101‑1 (MQ=60)
                                                AAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGNNN                                                       >  SRR4302518.9171455/1‑101 (MQ=60)
                                                 AAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTT                                                      <  SRR4302518.800671/101‑1 (MQ=60)
                                                  NNNGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTC                                                     <  SRR4302518.11429842/101‑1 (MQ=60)
                                                  AGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTC                                                     <  SRR4302518.7003943/101‑1 (MQ=60)
                                                  AGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTNNN                                                     >  SRR4302518.7882793/1‑101 (MQ=60)
                                                  AGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGGACGCGTTGTC                                                     >  SRR4302518.5551086/1‑101 (MQ=60)
                                                   NNNCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCC                                                    <  SRR4302518.12511622/101‑1 (MQ=60)
                                                     GGTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCNNN                                                  >  SRR4302518.12650510/1‑101 (MQ=60)
                                                     GCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAA‑AAAATGAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTAAGTGTATTCCAT                                                  >  SRR4302518.2064490/1‑101 (MQ=60)
                                                       TGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCANNN                                                >  SRR4302518.14250623/1‑101 (MQ=60)
                                                       NNNCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGC                                                <  SRR4302518.14795206/101‑1 (MQ=60)
                                                       NNNCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGC                                                <  SRR4302518.8417555/101‑1 (MQ=60)
                                                        GCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCC                                               >  SRR4302518.7314679/1‑101 (MQ=60)
                                                        GCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCC                                               <  SRR4302518.5712557/101‑1 (MQ=60)
                                                         cagcaAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCG                                              <  SRR4302518.1566137/96‑1 (MQ=60)
                                                         CCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCG                                              <  SRR4302518.3817715/101‑1 (MQ=60)
                                                          NNNCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCTCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGG                                             <  SRR4302518.10318572/101‑1 (MQ=60)
                                                          CGCCAGCAGCATTTTGTGCATCCTAGCCTTCCCGCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCACGCCGG                                             <  SRR4302518.2379277/101‑1 (MQ=60)
                                                          CGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGG                                             >  SRR4302518.6223748/1‑101 (MQ=60)
                                                          CGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAA‑AAAATCAGGACAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCNNN                                             >  SRR4302518.14961831/1‑101 (MQ=60)
                                                          CCCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCNNN                                             >  SRR4302518.8150288/1‑101 (MQ=60)
                                                           NNNAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTCGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGA                                            <  SRR4302518.11368000/101‑1 (MQ=60)
                                                           GCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGAgatcggaagagcgtcgtgtagggaaagagtgg                                            >  SRR4302518.4138638/1‑69 (MQ=60)
                                                           GCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCAAGATTGATAACAAAGTACGCGTTTTCCATGCCNNN                                            >  SRR4302518.13348511/1‑101 (MQ=60)
                                                            NNNGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGCT                                           <  SRR4302518.12385659/101‑1 (MQ=60)
                                                            CCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCtgatt                                           >  SRR4302518.6150859/1‑96 (MQ=60)
                                                            CCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGAT                                           >  SRR4302518.2814799/1‑101 (MQ=60)
                                                            CCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGAT                                           <  SRR4302518.309846/101‑1 (MQ=60)
                                                             CAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAATGACGCGTTTTCCATGCCGtnnn                                          >  SRR4302518.13376770/1‑97 (MQ=60)
                                                             CAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGACGGATG                                          >  SRR4302518.5309517/1‑101 (MQ=60)
                                                              CGCAGCCTTTTGCGCATCATAGTCTTCCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGC                                         <  SRR4302518.2639975/101‑1 (MQ=60)
                                                              AGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGC                                         >  SRR4302518.6465879/1‑101 (MQ=60)
                                                              AGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGC                                         >  SRR4302518.2719690/1‑101 (MQ=60)
                                                               NNNGCATTTTGCGCATCATAGTCTTCCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCG                                        <  SRR4302518.10103818/101‑1 (MQ=60)
                                                               GCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCG                                        <  SRR4302518.1148534/101‑1 (MQ=60)
                                                               GCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCG                                        >  SRR4302518.5740762/1‑101 (MQ=60)
                                                               GCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCG                                        <  SRR4302518.3164656/101‑1 (MQ=60)
                                                                CAGCATTTTGCGCATCATAGTCTTCCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGG                                       >  SRR4302518.3779052/1‑101 (MQ=60)
                                                                 NNNATTTTGCGCATCATAGTCTTCCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGC                                      <  SRR4302518.13427701/101‑1 (MQ=60)
                                                                 NNNATTTTGCGCATCATAGTCTTCCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGC                                      <  SRR4302518.11834394/101‑1 (MQ=60)
                                                                 AGCATTTTGCGTATCATAGTCTTCCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGC                                      <  SRR4302518.3113400/101‑1 (MQ=60)
                                                                 AGCATTTTGCGCATCATAGTCTTCCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCNNN                                      >  SRR4302518.11710609/1‑101 (MQ=60)
                                                                 AGCATTTTGCGCATCATAGTCTTCCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGC                                      >  SRR4302518.2012772/1‑101 (MQ=60)
                                                                  GCATTTTGCGCATCATAGTCTTCCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCG                                     >  SRR4302518.2257012/1‑101 (MQ=60)
                                                                   CATTTTGCGCATCATAGTCTTCCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGNNN                                    >  SRR4302518.9148929/1‑101 (MQ=60)
                                                                   CATTTTGCGCATCATAGTCTTCCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGT                                    <  SRR4302518.3941328/101‑1 (MQ=60)
                                                                     TTTTGCGCATCATAGTCTTCCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAA                                  >  SRR4302518.5225121/1‑101 (MQ=60)
                                                                     TTTTGCGCATCATAGTCTTCCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGNNN                                  >  SRR4302518.7554413/1‑101 (MQ=60)
                                                                      NNNGCGCATCATAGTCTTCCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAA                                 <  SRR4302518.11769221/101‑1 (MQ=60)
                                                                       TTGCGCATCATAGTCTTCCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAAC                                >  SRR4302518.2429895/1‑101 (MQ=60)
                                                                        TGCGCATCATAGTCTTCCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACA                               <  SRR4302518.6645183/101‑1 (MQ=60)
                                                                        TGCGCATCATAGTCTTCCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACA                               <  SRR4302518.6192194/101‑1 (MQ=60)
                                                                         GCGCATCATAGTCTTCCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGCT                                                                           <  SRR4302518.11624167/56‑1 (MQ=60)
                                                                         GCGCATCATAGTCTTCCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAANNN                              >  SRR4302518.14696591/1‑101 (MQ=60)
                                                                         GCGCATCATAGTCTTCCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACAC                              >  SRR4302518.5389326/1‑101 (MQ=60)
                                                                          CGCATCATAGTCTTCCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACNNN                             >  SRR4302518.8205195/1‑101 (MQ=60)
                                                                          CGCATCATAGTCTTCCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACNNN                             >  SRR4302518.7690988/1‑101 (MQ=60)
                                                                           GCATCATAGTCTTCCCTCGAGAA‑AAAATCAGGCCAGCATGTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACANNN                            >  SRR4302518.13200271/1‑101 (MQ=60)
                                                                           GCATCATAGTCTTCCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCT                            <  SRR4302518.3920559/101‑1 (MQ=60)
                                                                            CGTCATAGTCTTCCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACNNN                           >  SRR4302518.12663597/1‑101 (MQ=60)
                                                                             NNNATATTCTTCCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTA                          <  SRR4302518.13495854/101‑1 (MQ=60)
                                                                             NNNATAGTCTTCCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTA                          <  SRR4302518.9060766/101‑1 (MQ=60)
                                                                             ATCATAGTCTTCCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTA                          >  SRR4302518.7235188/1‑101 (MQ=60)
                                                                             ATCATAGTCTTCCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTA                          >  SRR4302518.1331067/1‑101 (MQ=60)
                                                                              TAATAGTCTTCCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTAT                         >  SRR4302518.560654/1‑101 (MQ=60)
                                                                              NNNTAGTCTTCCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTAT                         <  SRR4302518.10516734/101‑1 (MQ=60)
                                                                               CATAGTCTTCCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATC                        <  SRR4302518.3473204/101‑1 (MQ=60)
                                                                                NNNGTCTTCCCTCAAG‑AGAAAATCAGGCCAGCATTTGCCGTCCCCAGATTGATAACACAGTGCGCGTTTTCAATGCCGGATGCGGAGTAAACTCCTTCTCC                       <  SRR4302518.15027602/101‑1 (MQ=54)
                                                                                 TAGTCTCCCCTAAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCC                      <  SRR4302518.5444805/101‑1 (MQ=60)
                                                                                 NNNTCTTCCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCC                      <  SRR4302518.11595100/101‑1 (MQ=60)
                                                                                 NNNTCTTCCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCA                      <  SRR4302518.14378600/101‑1 (MQ=60)
                                                                                  NNNCTTCCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCG                     <  SRR4302518.10038658/101‑1 (MQ=60)
                                                                                  AGTCTTCCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCG                     >  SRR4302518.285865/1‑101 (MQ=60)
                                                                                  AGTCTTCCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGAGGCGGCGTAGACACCTTATCNNN                     >  SRR4302518.14663106/1‑101 (MQ=60)
                                                                                  AGGCTTCCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCAGGCAGGATGCGGCGTAAACACCTGATGCCG                     >  SRR4302518.902956/1‑101 (MQ=60)
                                                                                   NNNTTCCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGC                    <  SRR4302518.13728207/101‑1 (MQ=60)
                                                                                    TCTTCCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAATAACGCGTTTTCCATGCCGCATGCGGCGTAAACACCTTATCacnnn                   >  SRR4302518.14733360/1‑96 (MQ=60)
                                                                                     NNNCCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCT                  <  SRR4302518.8089367/101‑1 (MQ=60)
                                                                                     CTTCCCCCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACCCCTTATCCCGCCT                  <  SRR4302518.5671334/101‑1 (MQ=60)
                                                                                      TTCCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAACCACCTTATCCCccnnn                 >  SRR4302518.11551414/1‑96 (MQ=60)
                                                                                      TTCCCGCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTA                 <  SRR4302518.4082370/101‑1 (MQ=60)
                                                                                       TCCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTAC                >  SRR4302518.1105237/1‑101 (MQ=60)
                                                                                       NNNCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTAC                <  SRR4302518.8988846/101‑1 (MQ=60)
                                                                                        nnntcaagaA‑AAAAGCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACT               <  SRR4302518.12111832/92‑1 (MQ=60)
                                                                                        CCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACAGCTTATCCCGCCTNNN               >  SRR4302518.8907471/1‑101 (MQ=60)
                                                                                        CCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTNNN               >  SRR4302518.14153080/1‑101 (MQ=60)
                                                                                        CCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACT               <  SRR4302518.1503528/101‑1 (MQ=60)
                                                                                        CCCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACT               <  SRR4302518.3499654/101‑1 (MQ=60)
                                                                                         nnncaagaA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACCC              <  SRR4302518.11283661/93‑1 (MQ=60)
                                                                                         CCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTANNN              >  SRR4302518.12256057/1‑101 (MQ=60)
                                                                                         CCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTC              >  SRR4302518.4347232/1‑101 (MQ=60)
                                                                                         CCTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTC              >  SRR4302518.3260948/1‑101 (MQ=60)
                                                                                          ttcaagaA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACNNN             >  SRR4302518.14464368/8‑101 (MQ=60)
                                                                                          nnnaagaA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACCCCTTATCCCGCCTACTCG             <  SRR4302518.10055894/94‑1 (MQ=60)
                                                                                          nnnaagaA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTCG             <  SRR4302518.13756209/94‑1 (MQ=60)
                                                                                          nnnaagaA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTCG             <  SRR4302518.11072220/94‑1 (MQ=60)
                                                                                          CTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTCG             <  SRR4302518.3100821/101‑1 (MQ=60)
                                                                                          CTCAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACNNN             >  SRR4302518.10189909/1‑101 (MQ=60)
                                                                                          CTCAAGAA‑AAAAGCGGGCCATCATTTGGTGGCCCCAGATTGATAACAAAGTTCGCGTTTTCCATGCCGGATGCGGCctaaccccctccccccgcctactcg             <  SRR4302518.4624174/101‑26 (MQ=56)
                                                                                           ccaagaA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTCGA            <  SRR4302518.6551758/95‑1 (MQ=60)
                                                                                            CAAGAA‑AAAATCAGGCCCGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTCGAT           <  SRR4302518.4164863/101‑1 (MQ=60)
                                                                                            CAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTCGAT           <  SRR4302518.765146/101‑1 (MQ=60)
                                                                                            CAAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTCGAT           >  SRR4302518.7137203/1‑101 (MQ=60)
                                                                                             NNN‑A‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTCGATCG         <  SRR4302518.8950782/101‑1 (MQ=60)
                                                                                             NNN‑A‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTCGATCG         <  SRR4302518.9798807/101‑1 (MQ=60)
                                                                                             AAGAA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCTGCGTAAACACCTTATCCCGCCTACTCGATC          >  SRR4302518.4594904/1‑101 (MQ=60)
                                                                                              agaA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTCGATCG         >  SRR4302518.1965136/4‑101 (MQ=60)
                                                                                              agaA‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTCGATCG         >  SRR4302518.613945/4‑101 (MQ=60)
                                                                                              NN‑N‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTCGATCGT        <  SRR4302518.8780095/101‑1 (MQ=60)
                                                                                              NN‑N‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTAATCCCGCCTACTCGATCGT        <  SRR4302518.9219803/101‑1 (MQ=60)
                                                                                               N‑N‑NAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTCGATCGTG       <  SRR4302518.9457974/101‑1 (MQ=60)
                                                                                                 agAAAATCAGGCCCGCATTTGCCGGCCCCAGATTGGTAACAAAATACGCGTTTTCCACGCCTGCTGCGGCGTAAAAACCTTATCCCGCCTACTCGATCNNN       >  SRR4302518.11985229/3‑101 (MQ=60)
                                                                                                 N‑NNAATCAGGCTAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCTTACTCGATCGTGC      <  SRR4302518.14521166/101‑1 (MQ=60)
                                                                                                 A‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACCCCTTATCCCGCCTACTCGATCGTGC      <  SRR4302518.1393920/101‑1 (MQ=60)
                                                                                                 A‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGCTTTCCATGCCGGATGCGGCGTAAACACCTTATCACGCCTACTCGATCGNNN      >  SRR4302518.13230741/1‑101 (MQ=60)
                                                                                                   AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTCGATCGTGCA     >  SRR4302518.3373087/1‑101 (MQ=60)
                                                                                                   AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTCGATCGTGCA     <  SRR4302518.3676240/101‑1 (MQ=60)
                                                                                                    AAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTCGATCGTGCAT    >  SRR4302518.5178960/1‑101 (MQ=60)
                                                                                                     AATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTCGATCGTGCNNN   >  SRR4302518.10664723/1‑101 (MQ=60)
                                                                                                     AATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTCGATCGTGCNNN   >  SRR4302518.9646396/1‑101 (MQ=60)
                                                                                                     AATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTCGATCGTGCATC   >  SRR4302518.6269421/1‑101 (MQ=60)
                                                                                                     AATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGAACGCGTTTTCCATGCCGGATGCGGCGTAAACACATTATCGCGCCTACTCGATCGTGCATT   >  SRR4302518.920919/1‑101 (MQ=60)
                                                                                                      NNNAGGCCAGCATTTGCTGACCCCAGATTGAAAACAAAGTACGCGTTTTCCATGCCGGATGCGTCGTAAACCCCTTATCCCGCCTACTCGATCGTGCATCT  <  SRR4302518.13874310/101‑1 (MQ=60)
                                                                                                      ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTCGATCGTGCANNN  >  SRR4302518.12941035/1‑101 (MQ=60)
                                                                                                      |                                                                                                    
GCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAG‑A‑AAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTCGATCGTGCATCT  >  NC_000913/3099561‑3099761

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑