Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,099,661 (A)5→6 intergenic (‑19/+164) yggN ← / ← b2959 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,099,6561.A98.0% 926.8 / 7.6 249intergenic (‑14/+169)yggN/b2959hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (4/1);  new base A (112/132);  total (116/133)
Fisher's exact test for biased strand distribution p-value = 1.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

Only 100 of 279 total aligned reads displayed.

TGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAG‑AAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGC  >  NC_000913/3099564‑3099743
                                                                                             |                                                                                       
tGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAg                                                                                          >  2:5722738/1‑93 (MQ=255)
tGACGCTGCACTGGTAGTCAGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAg                                                                                          >  2:2900879/1‑93 (MQ=255)
   cGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAG‑aaa                                                                                      <  1:414710/93‑1 (MQ=255)
   cGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAG‑aaa                                                                                      <  1:3523097/93‑1 (MQ=255)
   cGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAG‑aaa                                                                                      <  1:1917397/93‑1 (MQ=255)
    gCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAG‑aaaa                                                                                     <  2:6174352/93‑1 (MQ=255)
     cTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAG‑aaaaa                                                                                    <  2:5731759/93‑1 (MQ=255)
      tGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAG‑aaaaaa                                                                                   >  1:4913380/1‑92 (MQ=255)
       gCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAG‑aaaaaat                                                                                  >  2:3642614/1‑91 (MQ=255)
        cACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAG‑aaaaaatc                                                                                 >  1:3696102/1‑90 (MQ=255)
           tGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAgg                                                                               >  1:1704422/1‑93 (MQ=255)
             gTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCTGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGcc                                                                             >  1:4444218/1‑93 (MQ=255)
                gTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGACAGc                                                                          >  1:1985250/1‑93 (MQ=255)
                 tCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCa                                                                         <  1:2927310/93‑1 (MQ=255)
                  cGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCAt                                                                        <  1:6135689/93‑1 (MQ=255)
                    gCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCAttt                                                                      >  1:1253729/1‑93 (MQ=255)
                    gCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCAttt                                                                      >  1:4368947/1‑93 (MQ=255)
                      gTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGc                                                                    <  1:5546061/93‑1 (MQ=255)
                      gTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGc                                                                    <  2:2972019/93‑1 (MQ=255)
                      gTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGc                                                                    <  2:5812712/93‑1 (MQ=255)
                       tGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCt                                                                   >  2:5536405/1‑93 (MQ=255)
                        gAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTg                                                                  <  2:4655752/93‑1 (MQ=255)
                        gAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTg                                                                  >  1:3056193/1‑93 (MQ=255)
                           cGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGcc                                                               <  1:5807865/93‑1 (MQ=255)
                            ggTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGccc                                                              <  2:428000/93‑1 (MQ=255)
                            ggTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGccc                                                              <  1:2159644/93‑1 (MQ=255)
                             gTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGcccc                                                             >  2:5567795/1‑93 (MQ=255)
                              tCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCa                                                            <  2:4455639/93‑1 (MQ=255)
                               cATTCCCGTCGCTGAAAGTCCTGCCGCCATCAGAATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAg                                                           <  2:5395182/93‑1 (MQ=255)
                                aTTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGa                                                          <  2:2800110/93‑1 (MQ=255)
                                 ttGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGAt                                                         >  1:1562604/1‑93 (MQ=255)
                                  tGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGAtt                                                        <  2:3133262/93‑1 (MQ=255)
                                   gCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTg                                                       >  1:4924862/1‑93 (MQ=255)
                                    ccGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGa                                                      >  1:4500659/1‑93 (MQ=255)
                                     cGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGAt                                                     <  2:2386885/93‑1 (MQ=255)
                                      gTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATa                                                    <  1:6050728/93‑1 (MQ=255)
                                       tCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATaa                                                   >  1:2585746/1‑93 (MQ=255)
                                       gcACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATaa                                                   <  1:1432392/92‑1 (MQ=255)
                                        cACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAAc                                                  >  2:5149921/1‑93 (MQ=255)
                                        cACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAAc                                                  >  2:3668985/1‑93 (MQ=255)
                                          cTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACaa                                                >  1:4649983/1‑93 (MQ=255)
                                          cTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACaa                                                <  2:4771384/93‑1 (MQ=255)
                                            gAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAg                                              <  2:6055295/93‑1 (MQ=255)
                                             aaaGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGt                                             <  1:337740/93‑1 (MQ=255)
                                             aaaGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGt                                             <  1:4264521/93‑1 (MQ=255)
                                              aaGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTa                                            >  2:1904785/1‑93 (MQ=255)
                                              aaGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTa                                            >  2:1129154/1‑93 (MQ=255)
                                              aaGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTa                                            <  2:3762400/93‑1 (MQ=255)
                                               aGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTAc                                           <  1:3840044/93‑1 (MQ=255)
                                               aGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTAc                                           >  1:103064/1‑93 (MQ=255)
                                                gTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTAcg                                          <  1:2252241/93‑1 (MQ=255)
                                                   ctgcCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGt                                       <  1:3940018/93‑1 (MQ=255)
                                                    tgcCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGtt                                      <  2:299569/93‑1 (MQ=255)
                                                    tgcCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGtt                                      <  1:2902348/93‑1 (MQ=255)
                                                     gccgccAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGttt                                     >  2:5478534/1‑93 (MQ=255)
                                                     gccgccAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGttt                                     <  1:114209/93‑1 (MQ=255)
                                                      ccgccAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGtttt                                    >  1:6169565/1‑93 (MQ=255)
                                                       cgccAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTc                                   <  1:5734258/93‑1 (MQ=255)
                                                        gccAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTcc                                  >  1:5918159/1‑93 (MQ=255)
                                                         ccAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCCGATTGATAACAAAGTACGCGTTTTcct                                 >  2:4336216/1‑92 (MQ=255)
                                                         ccAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCa                                 <  1:3246564/93‑1 (MQ=255)
                                                          cagcagCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCAt                                >  1:5965482/1‑93 (MQ=255)
                                                           agcagcATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATg                               >  2:3374263/1‑93 (MQ=255)
                                                           agcagcATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATg                               <  2:5267421/93‑1 (MQ=255)
                                                           agcagcATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGCTTTTCATg                               >  2:4542746/1‑93 (MQ=255)
                                                            gcagcaTTTTTCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGc                              <  2:1332523/93‑1 (MQ=255)
                                                            gcagcaTTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGc                              >  1:1455776/1‑93 (MQ=255)
                                                            gcagcaTTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGc                              <  1:2113229/93‑1 (MQ=255)
                                                             cagcaTTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGcc                             <  2:5654861/93‑1 (MQ=255)
                                                             cagcaTTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGcc                             >  2:1580681/1‑93 (MQ=255)
                                                               gcaTTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCgg                           >  1:5495185/1‑93 (MQ=255)
                                                               gcaTTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCgg                           >  2:599969/1‑93 (MQ=255)
                                                               gcaTTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCgg                           >  2:4052572/1‑93 (MQ=255)
                                                                caTTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGa                          <  1:5356224/93‑1 (MQ=255)
                                                                caTTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGa                          <  1:1017198/93‑1 (MQ=255)
                                                                 aTTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGAt                         <  1:2301774/93‑1 (MQ=255)
                                                                  ttttGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATg                        <  2:5928715/93‑1 (MQ=255)
                                                                  ttttGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATg                        >  2:2691738/1‑93 (MQ=255)
                                                                    ttGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATgcg                      <  2:668976/93‑1 (MQ=255)
                                                                    ttGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATgcg                      <  1:2501130/93‑1 (MQ=255)
                                                                     tGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATgcgg                     <  1:1320672/93‑1 (MQ=255)
                                                                     tGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATgcgg                     <  1:3763435/93‑1 (MQ=255)
                                                                     tGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATgcgg                     <  2:3516700/93‑1 (MQ=255)
                                                                       cgcATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATgcggcg                   >  1:2084238/1‑93 (MQ=255)
                                                                       cgcATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTACATGCCGGATgcggcg                   >  2:4014860/1‑93 (MQ=255)
                                                                           tcatAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTaaa               >  1:632790/1‑93 (MQ=255)
                                                                             atAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAaca             >  1:798220/1‑93 (MQ=255)
                                                                             atAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAaca             >  1:914070/1‑93 (MQ=255)
                                                                              tAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAacac            >  1:2817744/1‑93 (MQ=255)
                                                                               aGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACAcc           <  1:3559752/93‑1 (MQ=255)
                                                                                gTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCt          >  2:488889/1‑93 (MQ=255)
                                                                                gTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCt          <  2:5867072/93‑1 (MQ=255)
                                                                                gTCTCCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCt          <  2:2306750/93‑1 (MQ=255)
                                                                                  cTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTa        <  2:1055921/93‑1 (MQ=255)
                                                                                  cTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTa        >  1:4701378/1‑93 (MQ=255)
                                                                                   ttCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTAt       <  2:715329/93‑1 (MQ=255)
                                                                                    tCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTACACACCTTATc      >  2:203081/1‑93 (MQ=255)
                                                                                      ccTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATccc    >  1:5207052/1‑93 (MQ=255)
                                                                                       cTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCg   >  1:2710041/1‑93 (MQ=255)
                                                                                       cTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCg   <  2:1746824/93‑1 (MQ=255)
                                                                                             |                                                                                       
TGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAG‑AAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGC  >  NC_000913/3099564‑3099743

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAG‑AAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTCGATC  >  NC_000913/3099568‑3099753
                                                                                              |                                                                                            
GCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAG‑AAAAA                                                                                              >  SRR4302533.5548377/1‑94 (MQ=60)
 CTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAG‑AAAAAA                                                                                             <  SRR4302533.5359347/94‑1 (MQ=60)
 CTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAG‑AAAAAA                                                                                             >  SRR4302533.4943141/1‑94 (MQ=60)
 CTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAG‑AAAAAA                                                                                             >  SRR4302533.1145137/1‑94 (MQ=60)
  TGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAG‑AAAAAat                                                                                            <  SRR4302533.4533977/94‑3 (MQ=60)
   GCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAG‑AAAAAatc                                                                                           >  SRR4302533.3718805/1‑91 (MQ=60)
    CACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCA                                                                                           <  SRR4302533.2030923/94‑1 (MQ=60)
    CACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCA                                                                                           <  SRR4302533.4528289/94‑1 (MQ=60)
     ACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAG                                                                                          <  SRR4302533.2145185/94‑1 (MQ=60)
      CTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGG                                                                                         <  SRR4302533.2432053/94‑1 (MQ=60)
      CTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGG                                                                                         <  SRR4302533.2603513/94‑1 (MQ=60)
      CTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGG                                                                                         <  SRR4302533.2606928/94‑1 (MQ=60)
      CTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGG                                                                                         <  SRR4302533.323503/94‑1 (MQ=60)
      CTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGG                                                                                         >  SRR4302533.1714746/1‑94 (MQ=60)
        GGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCTGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCC                                                                                       >  SRR4302533.4471099/1‑94 (MQ=60)
        GGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCC                                                                                       <  SRR4302533.3157654/94‑1 (MQ=60)
           AGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGC                                                                                    >  SRR4302533.194122/1‑94 (MQ=60)
           AGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGACAGC                                                                                    >  SRR4302533.1997396/1‑94 (MQ=60)
             TCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCAT                                                                                  <  SRR4302533.2945222/94‑1 (MQ=60)
              CGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATT                                                                                 <  SRR4302533.6172658/94‑1 (MQ=60)
               GGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTT                                                                                >  SRR4302533.5693689/1‑94 (MQ=60)
               GGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTT                                                                                >  SRR4302533.1261225/1‑94 (MQ=60)
               GGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTT                                                                                >  SRR4302533.4395319/1‑94 (MQ=60)
                 CGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGC                                                                              <  SRR4302533.4439453/94‑1 (MQ=60)
                  GTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCT                                                                             <  SRR4302533.5579558/94‑1 (MQ=60)
                  GTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCT                                                                             >  SRR4302533.3457392/1‑94 (MQ=60)
                  GTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCT                                                                             >  SRR4302533.3929509/1‑94 (MQ=60)
                   TGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTG                                                                            >  SRR4302533.3074935/1‑94 (MQ=60)
                    GAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGG                                                                           <  SRR4302533.6191406/94‑1 (MQ=60)
                     AGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGC                                                                          >  SRR4302533.2216156/1‑94 (MQ=60)
                     AGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGC                                                                          >  SRR4302533.5923043/1‑94 (MQ=60)
                     AGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGC                                                                          <  SRR4302533.2113222/94‑1 (MQ=60)
                      GCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCC                                                                         <  SRR4302533.1319010/94‑1 (MQ=60)
                      GCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCC                                                                         <  SRR4302533.6297984/94‑1 (MQ=60)
                      GCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCC                                                                         >  SRR4302533.5790310/1‑94 (MQ=60)
                       CGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCC                                                                        <  SRR4302533.5842937/94‑1 (MQ=60)
                        GGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCC                                                                       >  SRR4302533.774237/1‑94 (MQ=60)
                        GGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCC                                                                       >  SRR4302533.2922072/1‑94 (MQ=60)
                        GGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCC                                                                       <  SRR4302533.2172940/94‑1 (MQ=60)
                        GGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCC                                                                       >  SRR4302533.1443647/1‑94 (MQ=60)
                         GTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCA                                                                      >  SRR4302533.1026753/1‑94 (MQ=60)
                            ATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGAT                                                                   >  SRR4302533.1572010/1‑94 (MQ=60)
                            ATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTGTGCGCATCATAGTCTTCCCTCAAGAAAAAAGCAGGCCAGCATTTGCTGGCCCCAGAT                                                                   <  SRR4302533.3763767/94‑1 (MQ=60)
                              TGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTG                                                                 >  SRR4302533.4954693/1‑94 (MQ=60)
                              TGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTG                                                                 <  SRR4302533.3754390/94‑1 (MQ=60)
                               GCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGA                                                                <  SRR4302533.1365061/94‑1 (MQ=60)
                               GCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGA                                                                >  SRR4302533.4527921/1‑94 (MQ=60)
                                 CGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATA                                                              >  SRR4302533.3298798/1‑94 (MQ=60)
                                  GTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAA                                                             <  SRR4302533.3990645/94‑1 (MQ=60)
                                  GTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAA                                                             <  SRR4302533.5918628/94‑1 (MQ=60)
                                  GTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAA                                                             >  SRR4302533.2601643/1‑94 (MQ=60)
                                  GTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAA                                                             <  SRR4302533.6087381/94‑1 (MQ=60)
                                  GTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAA                                                             <  SRR4302533.3184028/94‑1 (MQ=60)
                                   TCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAAC                                                            <  SRR4302533.4154272/94‑1 (MQ=60)
                                   GCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAAC                                                            <  SRR4302533.1441048/94‑1 (MQ=60)
                                    CACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACA                                                           >  SRR4302533.4892292/1‑94 (MQ=60)
                                     ACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAA                                                          >  SRR4302533.2733903/1‑94 (MQ=60)
                                     ACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAA                                                          >  SRR4302533.4678158/1‑94 (MQ=60)
                                     ACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAA                                                          >  SRR4302533.4976632/1‑94 (MQ=60)
                                     ACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAA                                                          <  SRR4302533.3038520/94‑1 (MQ=60)
                                      CTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAA                                                         >  SRR4302533.1599663/1‑94 (MQ=60)
                                      CTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTACCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAA                                                         >  SRR4302533.4475366/1‑94 (MQ=60)
                                         AAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTA                                                      >  SRR4302533.3891618/1‑94 (MQ=60)
                                         AAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTA                                                      <  SRR4302533.339803/94‑1 (MQ=60)
                                         AAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTA                                                      <  SRR4302533.4290206/94‑1 (MQ=60)
                                          AAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTAC                                                     >  SRR4302533.103709/1‑94 (MQ=60)
                                          AAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTAC                                                     <  SRR4302533.3573536/94‑1 (MQ=60)
                                          AAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTAC                                                     <  SRR4302533.2921257/94‑1 (MQ=60)
                                           AGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACG                                                    <  SRR4302533.4954254/94‑1 (MQ=60)
                                           AGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACG                                                    >  SRR4302533.6014188/1‑94 (MQ=60)
                                           AGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACG                                                    <  SRR4302533.3863503/94‑1 (MQ=60)
                                            GTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGC                                                   <  SRR4302533.5483098/94‑1 (MQ=60)
                                            GTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGC                                                   <  SRR4302533.2266167/94‑1 (MQ=60)
                                             TGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCG                                                  <  SRR4302533.5770197/94‑1 (MQ=60)
                                               CTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTT                                                <  SRR4302533.3964062/94‑1 (MQ=60)
                                               CTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTT                                                <  SRR4302533.1325449/94‑1 (MQ=60)
                                               CTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTT                                                <  SRR4302533.3591614/94‑1 (MQ=60)
                                                TGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTT                                               <  SRR4302533.2920176/94‑1 (MQ=60)
                                                TGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTT                                               <  SRR4302533.3555663/94‑1 (MQ=60)
                                                TGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTT                                               <  SRR4302533.1505564/94‑1 (MQ=60)
                                                 GCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTT                                              <  SRR4302533.114933/94‑1 (MQ=60)
                                                 GCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTT                                              >  SRR4302533.6206649/1‑94 (MQ=60)
                                                  CCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTC                                             >  SRR4302533.3441060/1‑94 (MQ=60)
                                                   CGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCC                                            >  SRR4302533.5953924/1‑94 (MQ=60)
                                                   CGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCC                                            >  SRR4302533.216279/1‑94 (MQ=60)
                                                   CGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCC                                            <  SRR4302533.5768875/94‑1 (MQ=60)
                                                   CGCCAGCAGCATTTTGCGAATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCC                                            >  SRR4302533.4359644/1‑94 (MQ=60)
                                                    GCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCA                                           >  SRR4302533.5298055/1‑94 (MQ=60)
                                                    GCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCA                                           >  SRR4302533.2456787/1‑94 (MQ=60)
                                                     CCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCAT                                          <  SRR4302533.1251950/94‑1 (MQ=60)
                                                     CCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCAT                                          >  SRR4302533.6001581/1‑94 (MQ=60)
                                                     CCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCAT                                          <  SRR4302533.3266580/94‑1 (MQ=60)
                                                     CCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCAT                                          >  SRR4302533.6181800/1‑94 (MQ=60)
                                                      CAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATG                                         >  SRR4302533.4234324/1‑94 (MQ=60)
                                                      CAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATG                                         <  SRR4302533.5986421/94‑1 (MQ=60)
                                                      CAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATG                                         >  SRR4302533.3798594/1‑94 (MQ=60)
                                                      CAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATG                                         >  SRR4302533.4524709/1‑94 (MQ=60)
                                                       AGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGC                                        <  SRR4302533.2123041/94‑1 (MQ=60)
                                                       AGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGC                                        >  SRR4302533.1464553/1‑94 (MQ=60)
                                                        GCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCC                                       <  SRR4302533.2126263/94‑1 (MQ=60)
                                                        GCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCC                                       <  SRR4302533.2560693/94‑1 (MQ=60)
                                                        GCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCC                                       <  SRR4302533.4274209/94‑1 (MQ=60)
                                                        GCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCC                                       <  SRR4302533.615092/94‑1 (MQ=60)
                                                        GCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCC                                       <  SRR4302533.3074878/94‑1 (MQ=60)
                                                        GCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCC                                       >  SRR4302533.6166698/1‑94 (MQ=60)
                                                         CAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCG                                      >  SRR4302533.1236907/1‑94 (MQ=60)
                                                          AGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGG                                     >  SRR4302533.5528332/1‑94 (MQ=60)
                                                          AGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGG                                     >  SRR4302533.1902246/1‑94 (MQ=60)
                                                            CATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGAT                                   <  SRR4302533.5388375/94‑1 (MQ=60)
                                                            CATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGAT                                   <  SRR4302533.1023177/94‑1 (MQ=60)
                                                            CATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGAT                                   <  SRR4302533.5684615/94‑1 (MQ=60)
                                                            CATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGAT                                   <  SRR4302533.1466391/94‑1 (MQ=60)
                                                             ATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATG                                  <  SRR4302533.2316037/94‑1 (MQ=60)
                                                             ATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATG                                  <  SRR4302533.2063728/94‑1 (MQ=60)
                                                               TTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCG                                >  SRR4302533.2722957/1‑94 (MQ=60)
                                                                TTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGG                               <  SRR4302533.2516557/94‑1 (MQ=60)
                                                                 TGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGC                              <  SRR4302533.3786505/94‑1 (MQ=60)
                                                                 TGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGC                              <  SRR4302533.1328601/94‑1 (MQ=60)
                                                                  GCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCG                             <  SRR4302533.842724/94‑1 (MQ=60)
                                                                  GCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCG                             >  SRR4302533.2097070/1‑94 (MQ=60)
                                                                    GCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTA                           <  SRR4302533.5964254/94‑1 (MQ=60)
                                                                      ATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAGA                         >  SRR4302533.2800019/1‑94 (MQ=60)
                                                                      ATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAA                         >  SRR4302533.636513/1‑94 (MQ=60)
                                                                      ATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAA                         <  SRR4302533.3700097/94‑1 (MQ=60)
                                                                      ATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAA                         >  SRR4302533.4704927/1‑94 (MQ=60)
                                                                        CATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACA                       >  SRR4302533.802916/1‑94 (MQ=60)
                                                                        CATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACA                       >  SRR4302533.919445/1‑94 (MQ=60)
                                                                         ATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACAC                      >  SRR4302533.2835086/1‑94 (MQ=60)
                                                                         ATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACAC                      >  SRR4302533.2655535/1‑94 (MQ=60)
                                                                           AGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCT                    <  SRR4302533.10899/94‑1 (MQ=60)
                                                                           AGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCT                    <  SRR4302533.3581685/94‑1 (MQ=60)
                                                                            GTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTT                   <  SRR4302533.1912552/94‑1 (MQ=60)
                                                                            GTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTT                   <  SRR4302533.716866/94‑1 (MQ=60)
                                                                             TCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTA                  >  SRR4302533.4729882/1‑94 (MQ=60)
                                                                              CTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTAT                 >  SRR4302533.4712413/1‑94 (MQ=60)
                                                                              CTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTAT                 <  SRR4302533.4345925/94‑1 (MQ=60)
                                                                              CTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTAT                 >  SRR4302533.2509826/1‑94 (MQ=60)
                                                                              CTACCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTAT                 <  SRR4302533.4927729/94‑1 (MQ=60)
                                                                               TTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATC                >  SRR4302533.1584189/1‑94 (MQ=60)
                                                                               TTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATC                >  SRR4302533.2289105/1‑94 (MQ=60)
                                                                                TCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCC               <  SRR4302533.1005513/94‑1 (MQ=60)
                                                                                 CCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCC              >  SRR4302533.5238423/1‑94 (MQ=60)
                                                                                  CCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCG             >  SRR4302533.2726675/1‑94 (MQ=60)
                                                                                  CCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCC             >  SRR4302533.2238488/1‑94 (MQ=60)
                                                                                    TCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCC           <  SRR4302533.284436/94‑1 (MQ=60)
                                                                                    TCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCC           <  SRR4302533.6250427/94‑1 (MQ=60)
                                                                                      AAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTA         >  SRR4302533.2003599/1‑94 (MQ=60)
                                                                                       agaAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTAC        >  SRR4302533.869551/4‑94 (MQ=60)
                                                                                       agaAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTAC        >  SRR4302533.1908702/4‑94 (MQ=60)
                                                                                       AA‑AAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACT       <  SRR4302533.5080107/94‑1 (MQ=60)
                                                                                        gaAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACT       <  SRR4302533.2173866/92‑1 (MQ=60)
                                                                                        gaAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACT       <  SRR4302533.5174825/92‑1 (MQ=60)
                                                                                        gaAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACT       <  SRR4302533.326681/92‑1 (MQ=60)
                                                                                        A‑AAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTC      <  SRR4302533.3074146/94‑1 (MQ=60)
                                                                                          AAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTCG     <  SRR4302533.4428444/94‑1 (MQ=60)
                                                                                          AAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTCG     <  SRR4302533.2416961/94‑1 (MQ=60)
                                                                                             AATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTCGATC  <  SRR4302533.3617725/94‑1 (MQ=60)
                                                                                             AATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTCGATC  <  SRR4302533.3350275/94‑1 (MQ=60)
                                                                                              |                                                                                            
GCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAG‑AAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTCGATC  >  NC_000913/3099568‑3099753

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑