Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,125,359 T→C K226K (AAA→AAG)  glcD ← glycolate oxidase subunit D

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,125,3590TC100.0% 731.8 / NA 230K226K (AAA→AAG) glcDglycolate oxidase subunit D
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (122/108);  total (122/108)

Only 100 of 245 total aligned reads displayed.

GGCGATGATGTCACCAACCGCAAGTCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGTTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGCGCGTC  >  NC_000913/3125269‑3125448
                                                                                          |                                                                                         
ggCGATGATGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGctt                                                                                         <  1:3642138/93‑1 (MQ=255)
 gCGATGATGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGcttt                                                                                        >  1:5067959/1‑93 (MQ=255)
 gCGATGATGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGcttt                                                                                        >  2:5548144/1‑93 (MQ=255)
  cGATGATGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTa                                                                                       <  2:4200899/93‑1 (MQ=255)
   gatgatGTCACCAACCGCAAGCCCGGCTTTTTTTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTAc                                                                                      >  1:1452820/1‑93 (MQ=255)
   gatgatGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTAc                                                                                      <  1:530985/93‑1 (MQ=255)
    atgatgTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTAcc                                                                                     >  1:1207589/1‑93 (MQ=255)
      gatgTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGAGCCACGGGCGGCTTCGGCAGCAGCTTTACCGt                                                                                   >  2:4522893/1‑93 (MQ=255)
         gTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCAc                                                                                <  2:6276056/93‑1 (MQ=255)
          tCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACt                                                                               >  2:2858949/1‑93 (MQ=255)
           cACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACtt                                                                              >  1:4505313/1‑93 (MQ=255)
           cACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACtt                                                                              <  1:3035768/93‑1 (MQ=255)
            accaccCGCAAGCCCGGCTGTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTc                                                                             <  1:2973636/93‑1 (MQ=255)
            aCCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTc                                                                             <  2:5714471/93‑1 (MQ=255)
            aCCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTc                                                                             <  1:6203825/93‑1 (MQ=255)
            aCCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTc                                                                             >  2:1869779/1‑93 (MQ=255)
             ccAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCg                                                                            >  1:2693342/1‑93 (MQ=255)
             ccAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCg                                                                            <  1:5876830/93‑1 (MQ=255)
              cAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGTGCGGCTTCGGCAGCAGCTTTACCGTCACTTCgg                                                                           >  2:4345257/1‑93 (MQ=255)
               aaCCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCggt                                                                          <  2:4426700/93‑1 (MQ=255)
               aaCCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCggt                                                                          >  2:1441342/1‑93 (MQ=255)
                aCCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCggtg                                                                         <  2:1729974/93‑1 (MQ=255)
                aCCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCggtg                                                                         <  2:5128991/93‑1 (MQ=255)
                 ccGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCggtgg                                                                        <  1:2901058/93‑1 (MQ=255)
                   gCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCCCTTCGGTGGTc                                                                      >  2:246293/1‑93 (MQ=255)
                   gCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTc                                                                      >  2:4658967/1‑93 (MQ=255)
                     aaGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCAc                                                                    >  2:4727644/1‑93 (MQ=255)
                      agcccGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACg                                                                   >  2:1330969/4‑93 (MQ=255)
                      agcccGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACg                                                                   >  2:1286938/4‑93 (MQ=255)
                       gcccGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTGACGc                                                                  >  1:1394055/3‑93 (MQ=255)
                        cccGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGcc                                                                 >  1:4667730/2‑93 (MQ=255)
                          cGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCgc                                                               >  2:3220621/1‑92 (MQ=255)
                          cGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGa                                                               >  2:2237756/1‑93 (MQ=255)
                          cGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGa                                                               >  1:5418523/1‑93 (MQ=255)
                          cGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACAGTCACTTCGGTGGGCACGCCGa                                                               >  1:315610/1‑93 (MQ=255)
                            gCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTGGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGc                                                             <  2:2107985/93‑1 (MQ=255)
                            gCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGc                                                             >  1:730426/1‑93 (MQ=255)
                            gCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGc                                                             >  1:4764891/1‑93 (MQ=255)
                                tttCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATAc                                                         <  2:895620/93‑1 (MQ=255)
                                 ttCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATAcc                                                        <  2:1583679/93‑1 (MQ=255)
                                 ttCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATAcc                                                        >  1:215983/1‑93 (MQ=255)
                                  tCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCt                                                       <  1:3265212/93‑1 (MQ=255)
                                   cTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCtt                                                      <  2:2123481/93‑1 (MQ=255)
                                   cTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCtt                                                      >  1:3776796/1‑93 (MQ=255)
                                    tACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTc                                                     <  2:5588176/93‑1 (MQ=255)
                                     aCCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTcc                                                    <  2:5073113/93‑1 (MQ=255)
                                       cGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGa                                                  <  2:6016217/93‑1 (MQ=255)
                                           tCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCg                                              >  1:2267936/1‑93 (MQ=255)
                                             aaaGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGt                                            >  2:4761468/1‑93 (MQ=255)
                                             aaaGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGt                                            >  2:1172266/1‑93 (MQ=255)
                                              aaGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTg                                           <  2:351291/93‑1 (MQ=255)
                                                gCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATCCCTTCCGATCCGGTGaa                                         >  1:776613/1‑93 (MQ=255)
                                                gCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGaa                                         <  2:1974727/93‑1 (MQ=255)
                                                 cTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAAc                                        <  1:156141/93‑1 (MQ=255)
                                                   ggCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAg                                      >  2:5782385/1‑93 (MQ=255)
                                                    gCTAACAGAGCCCGCGCCACGGGCGGCTTCCGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAgc                                     <  1:492360/93‑1 (MQ=255)
                                                     cTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAgcg                                    >  1:6025458/1‑93 (MQ=255)
                                                      tAACAGACCCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAgcgc                                   <  1:557804/93‑1 (MQ=255)
                                                      tAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAgcgc                                   >  2:5513098/1‑93 (MQ=255)
                                                       aaCAGACCCCGCGACACGGGCGGCTTCGGCAGCAGCTTTACGGTCACTACGGTGTTCACGCGGAGCATACCTCCCGATCCGGTGAACAGCGcc                                  <  1:2420507/93‑1 (MQ=255)
                                                       aaCAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGcc                                  >  2:3715320/1‑93 (MQ=255)
                                                       aaCAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGcc                                  >  1:4798222/1‑93 (MQ=255)
                                                       aaCAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGcc                                  >  1:6129515/1‑93 (MQ=255)
                                                        aCAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCca                                 >  1:64228/1‑93 (MQ=255)
                                                         cAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCcag                                >  1:1617308/1‑93 (MQ=255)
                                                          aGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCcagc                               >  1:2469874/1‑93 (MQ=255)
                                                           gatcccGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCCCGCCGAGCATACCTTCCGATCCGGTGAACAGCGCcagca                              <  2:5420865/90‑1 (MQ=255)
                                                            aaCCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCcagcag                             >  2:1822036/1‑93 (MQ=255)
                                                            aaCCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCcagcag                             <  1:889445/93‑1 (MQ=255)
                                                             aCCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAgg                            <  2:853510/93‑1 (MQ=255)
                                                             aCCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGACGAGCATACCTTCCGATCCGGTGAACGGCGCCAGAAgg                            >  1:1267641/1‑93 (MQ=255)
                                                              cccGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGt                           >  2:5884064/1‑93 (MQ=255)
                                                              cccGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGt                           <  2:2634083/93‑1 (MQ=255)
                                                                cgcgCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCa                         >  1:52316/1‑93 (MQ=255)
                                                                 gcgcCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCaa                        >  1:3734009/1‑93 (MQ=255)
                                                                   gcCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCaaaa                      >  2:5322900/1‑93 (MQ=255)
                                                                    ccACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAAc                     >  1:1102612/1‑93 (MQ=255)
                                                                    ccACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAAc                     <  2:3644848/93‑1 (MQ=255)
                                                                    ccACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAAc                     <  1:3511061/93‑1 (MQ=255)
                                                                      aCGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCa                   <  1:968499/93‑1 (MQ=255)
                                                                         ggcggcTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCGAAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAggg                >  1:5142240/1‑92 (MQ=255)
                                                                         ggcggcTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGt                <  1:2444958/93‑1 (MQ=255)
                                                                         ggcggcTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGt                <  2:2265445/93‑1 (MQ=255)
                                                                         ggcggcTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGt                >  1:187037/1‑93 (MQ=255)
                                                                          gcggcTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCCCGCCGAGCATCCCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTg               <  1:4080994/93‑1 (MQ=255)
                                                                           cggcTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGa              <  2:2741527/93‑1 (MQ=255)
                                                                            ggcTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGaa             >  2:3133709/1‑93 (MQ=255)
                                                                             gcTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGATCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAAt            >  1:3375186/1‑93 (MQ=255)
                                                                             gcTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAAt            >  2:2793650/1‑93 (MQ=255)
                                                                             gcTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAAt            >  1:2220552/1‑93 (MQ=255)
                                                                             gcTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAAt            >  1:4315164/1‑93 (MQ=255)
                                                                              cTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATc           >  2:3091392/1‑93 (MQ=255)
                                                                              cTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATc           <  2:3499910/93‑1 (MQ=255)
                                                                               ttCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATcc          >  1:5700092/1‑93 (MQ=255)
                                                                                tCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCa         <  2:1012647/93‑1 (MQ=255)
                                                                                tCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCa         <  1:3220621/93‑1 (MQ=255)
                                                                                 cGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCTATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAg        >  2:72398/1‑93 (MQ=255)
                                                                                 cGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAg        >  1:3351085/1‑93 (MQ=255)
                                                                                     agcagcTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGcgcg    >  1:3550228/1‑93 (MQ=255)
                                                                                     agcagcTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGcgcg    >  2:238019/1‑93 (MQ=255)
                                                                                          |                                                                                         
GGCGATGATGTCACCAACCGCAAGTCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGTTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGCGCGTC  >  NC_000913/3125269‑3125448

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGCGATGATGTCACCAACCGCAAGTCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGTTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGCGCGTCCGAT  >  NC_000913/3125269‑3125452
                                                                                          |                                                                                             
GGCGATGATGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTT                                                                                            >  SRR4302533.5098564/1‑94 (MQ=60)
GGCGATGATGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTT                                                                                            <  SRR4302533.3106375/94‑1 (MQ=60)
GGCGATGATGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTT                                                                                            <  SRR4302533.3664533/94‑1 (MQ=60)
 GCGATGATGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTA                                                                                           <  SRR4302533.702908/94‑1 (MQ=60)
  CGATGATGTCACCAACCGCAAGCCCGGCTTTTTTTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTAC                                                                                          >  SRR4302533.1461585/1‑94 (MQ=60)
   GATGATGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACC                                                                                         >  SRR4302533.1214837/1‑94 (MQ=60)
   GATGATGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACC                                                                                         <  SRR4302533.534143/94‑1 (MQ=60)
   GATGATGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACC                                                                                         >  SRR4302533.5060566/1‑94 (MQ=60)
   GATGATGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACC                                                                                         >  SRR4302533.3657912/1‑94 (MQ=60)
   GATGATGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACC                                                                                         <  SRR4302533.5868519/94‑1 (MQ=60)
    ATGATGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCG                                                                                        >  SRR4302533.1379158/1‑94 (MQ=60)
        TGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCAC                                                                                    >  SRR4302533.1699059/1‑94 (MQ=60)
          TCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTT                                                                                  >  SRR4302533.4532603/1‑94 (MQ=60)
          TCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTT                                                                                  <  SRR4302533.4236522/94‑1 (MQ=60)
          TCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTT                                                                                  >  SRR4302533.6003513/1‑94 (MQ=60)
           CACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTC                                                                                 <  SRR4302533.3054344/94‑1 (MQ=60)
           CACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTC                                                                                 >  SRR4302533.3513979/1‑94 (MQ=60)
            ACCACCCGCAAGCCCGGCTGTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCG                                                                                <  SRR4302533.2991830/94‑1 (MQ=60)
            ACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACNCGCGCCACGGGCGGNTTCNNNANCAGCTTTACCGNNNNTNCG                                                                                >  SRR4302533.5523998/1‑94 (MQ=60)
            ACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCG                                                                                >  SRR4302533.2709844/1‑94 (MQ=60)
            ACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCG                                                                                <  SRR4302533.6241030/94‑1 (MQ=60)
            ACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCG                                                                                >  SRR4302533.1906547/1‑94 (MQ=60)
             CCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAGCAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGG                                                                               >  SRR4302533.3394483/1‑94 (MQ=60)
             CCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGG                                                                               <  SRR4302533.5912356/94‑1 (MQ=60)
               AACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTG                                                                             <  SRR4302533.2633084/94‑1 (MQ=60)
                ACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGG                                                                            <  SRR4302533.957770/94‑1 (MQ=60)
                ACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGG                                                                            >  SRR4302533.809825/1‑94 (MQ=60)
                 CCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGT                                                                           <  SRR4302533.2918880/94‑1 (MQ=60)
                    CAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCAC                                                                        <  SRR4302533.3557847/94‑1 (MQ=60)
                     AAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACG                                                                       >  SRR4302533.2627948/1‑94 (MQ=60)
                     AAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACG                                                                       <  SRR4302533.4043837/94‑1 (MQ=60)
                     AAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACG                                                                       <  SRR4302533.740390/94‑1 (MQ=60)
                      AGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTGACGC                                                                      >  SRR4302533.1402456/1‑94 (MQ=60)
                       GCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCC                                                                     <  SRR4302533.4756878/94‑1 (MQ=60)
                       GCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCC                                                                     >  SRR4302533.4696010/1‑94 (MQ=60)
                       GCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCC                                                                     <  SRR4302533.5168145/94‑1 (MQ=60)
                         CCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGA                                                                   >  SRR4302533.365046/1‑94 (MQ=60)
                         CCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGA                                                                   >  SRR4302533.5451116/1‑94 (MQ=60)
                         CCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACAGTCACTTCGGTGGGCACGCCGA                                                                   >  SRR4302533.317537/1‑94 (MQ=60)
                          CGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAG                                                                  >  SRR4302533.1249337/1‑94 (MQ=60)
                          CGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAG                                                                  >  SRR4302533.2223820/1‑94 (MQ=60)
                           GGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGC                                                                 >  SRR4302533.4793788/1‑94 (MQ=60)
                           GGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGC                                                                 >  SRR4302533.734731/1‑94 (MQ=60)
                                TTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACC                                                            >  SRR4302533.217341/1‑94 (MQ=60)
                                  TCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTT                                                          <  SRR4302533.2908847/94‑1 (MQ=60)
                                  TCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTT                                                          >  SRR4302533.3799943/1‑94 (MQ=60)
                                  TCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTT                                                          <  SRR4302533.3285359/94‑1 (MQ=60)
                                     ACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCG                                                       <  SRR4302533.3739972/94‑1 (MQ=60)
                                      CCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGT                                                      >  SRR4302533.4914179/1‑94 (MQ=60)
                                       CGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCCGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGAT                                                     >  SRR4302533.3242396/1‑94 (MQ=60)
                                       CGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGAT                                                     >  SRR4302533.1393496/1‑94 (MQ=60)
                                        GAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATC                                                    <  SRR4302533.4821092/94‑1 (MQ=60)
                                        GAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATC                                                    >  SRR4302533.1579206/1‑94 (MQ=60)
                                          GTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCG                                                  <  SRR4302533.5700856/94‑1 (MQ=60)
                                          GTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCG                                                  >  SRR4302533.2281966/1‑94 (MQ=60)
                                          GTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCG                                                  >  SRR4302533.2993821/1‑94 (MQ=60)
                                           TCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGG                                                 >  SRR4302533.6220988/1‑94 (MQ=60)
                                            CAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGT                                                >  SRR4302533.533508/1‑94 (MQ=60)
                                            CAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGT                                                >  SRR4302533.4336945/1‑94 (MQ=60)
                                             AAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTG                                               <  SRR4302533.4440027/94‑1 (MQ=60)
                                             AAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTG                                               <  SRR4302533.3175058/94‑1 (MQ=60)
                                             AAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTG                                               <  SRR4302533.5332082/94‑1 (MQ=60)
                                             AAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTG                                               <  SRR4302533.6207962/94‑1 (MQ=60)
                                               AGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATCCCTTCCGATCCGGTGAA                                             >  SRR4302533.781179/1‑94 (MQ=60)
                                                 CTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACA                                           <  SRR4302533.157136/94‑1 (MQ=60)
                                                 CTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACA                                           >  SRR4302533.1449876/1‑94 (MQ=60)
                                                  TGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAG                                          >  SRR4302533.3289246/1‑94 (MQ=60)
                                                    GCTAACAGAGCCCGCGCCACGGGCGGCTTCCGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCG                                        <  SRR4302533.495302/94‑1 (MQ=60)
                                                    GCTAACAGACCCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATCCCTTCCGATCCGGTGAACAGCG                                        <  SRR4302533.1546475/94‑1 (MQ=60)
                                                    GCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCG                                        <  SRR4302533.803382/94‑1 (MQ=60)
                                                    GCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCG                                        >  SRR4302533.6061969/1‑94 (MQ=60)
                                                      TAACAGACCCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCC                                      <  SRR4302533.561117/94‑1 (MQ=60)
                                                      TAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCC                                      >  SRR4302533.4827285/1‑94 (MQ=60)
                                                      TAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCC                                      >  SRR4302533.6166451/1‑94 (MQ=60)
                                                      TAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCCGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCC                                      >  SRR4302533.2468632/1‑94 (MQ=60)
                                                       AACAGACCCCGCGACACGGGCGGCTTCGGCAGCAGCTTTACGGTCACTACGGTGTTCACGCGGAGCATACCTCCCGATCCGGTGAACAGCGCCA                                     <  SRR4302533.2435455/94‑1 (MQ=60)
                                                       AACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCA                                     >  SRR4302533.64645/1‑94 (MQ=60)
                                                        ACAGAACCCGCGCGACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAG                                    <  SRR4302533.6051298/94‑1 (MQ=60)
                                                        ACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAG                                    >  SRR4302533.1627108/1‑94 (MQ=60)
                                                         CAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGC                                   >  SRR4302533.2485110/1‑94 (MQ=60)
                                                           GAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAG                                 <  SRR4302533.3517774/94‑1 (MQ=60)
                                                           GAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAG                                 <  SRR4302533.2973494/94‑1 (MQ=60)
                                                            AACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGG                                <  SRR4302533.894669/94‑1 (MQ=60)
                                                            AACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGACGAGCATACCTTCCGATCCGGTGAACGGCGCCAGAAGG                                >  SRR4302533.1275225/1‑94 (MQ=60)
                                                              CCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTC                              <  SRR4302533.3789122/94‑1 (MQ=60)
                                                               CCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCA                             >  SRR4302533.52670/1‑94 (MQ=60)
                                                               CCGCGCCACGGGCGCCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCA                             <  SRR4302533.5368600/94‑1 (MQ=60)
                                                                CGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAA                            >  SRR4302533.3756913/1‑94 (MQ=60)
                                                                   GCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAAC                         <  SRR4302533.5482384/94‑1 (MQ=60)
                                                                   GCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAAC                         >  SRR4302533.1109142/1‑94 (MQ=60)
                                                                    CCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACC                        <  SRR4302533.3532704/94‑1 (MQ=60)
                                                                    CCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACC                        >  SRR4302533.2274316/1‑94 (MQ=60)
                                                                     CACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCA                       >  SRR4302533.3244904/1‑94 (MQ=60)
                                                                     CACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCA                       >  SRR4302533.2454213/1‑94 (MQ=60)
                                                                     CACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCA                       >  SRR4302533.642983/1‑94 (MQ=60)
                                                                      ACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAG                      <  SRR4302533.974181/94‑1 (MQ=60)
                                                                      ACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAG                      <  SRR4302533.4774273/94‑1 (MQ=60)
                                                                      ACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAG                      >  SRR4302533.5490006/1‑94 (MQ=60)
                                                                        GGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCGAAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGG                    >  SRR4302533.5173318/1‑94 (MQ=60)
                                                                        GGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGT                    <  SRR4302533.975937/94‑1 (MQ=60)
                                                                        GGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGT                    >  SRR4302533.188199/1‑94 (MQ=60)
                                                                        GGGCGGCTTCGGCAGCAGCTGTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGT                    <  SRR4302533.3220972/94‑1 (MQ=60)
                                                                         GGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTG                   <  SRR4302533.2460051/94‑1 (MQ=60)
                                                                          GCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCCCGCCGAGCATCCCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGA                  <  SRR4302533.4105839/94‑1 (MQ=60)
                                                                            GGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGATCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAAT                >  SRR4302533.3396032/1‑94 (MQ=60)
                                                                            GGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAAT                >  SRR4302533.2234260/1‑94 (MQ=60)
                                                                            GGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAAT                <  SRR4302533.2988808/94‑1 (MQ=60)
                                                                            GGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAAT                >  SRR4302533.4341146/1‑94 (MQ=60)
                                                                              CTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCC              >  SRR4302533.5734514/1‑94 (MQ=60)
                                                                              CTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCC              <  SRR4302533.1207099/94‑1 (MQ=60)
                                                                               TTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCA             >  SRR4302533.1640597/1‑94 (MQ=60)
                                                                               TTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCA             <  SRR4302533.3790453/94‑1 (MQ=60)
                                                                                TCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAG            <  SRR4302533.2452082/94‑1 (MQ=60)
                                                                                TCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAG            >  SRR4302533.3371786/1‑94 (MQ=60)
                                                                                TCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAG            <  SRR4302533.3240481/94‑1 (MQ=60)
                                                                                 CGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGC           <  SRR4302533.5102400/94‑1 (MQ=60)
                                                                                  GGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGCG          >  SRR4302533.5187472/1‑94 (MQ=60)
                                                                                   GCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGCGC         >  SRR4302533.690759/1‑94 (MQ=60)
                                                                                    CAGCAGCTTTACCGTCACTTCGGTGGTCGCGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGCGCG        <  SRR4302533.611820/94‑1 (MQ=60)
                                                                                    CAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGCGCG        >  SRR4302533.3572100/1‑94 (MQ=60)
                                                                                    CAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGCGCG        >  SRR4302533.2434139/1‑94 (MQ=60)
                                                                                     AGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGCGCGT       >  SRR4302533.1486772/1‑94 (MQ=60)
                                                                                     AGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGCGCGT       <  SRR4302533.2177296/94‑1 (MQ=60)
                                                                                      GCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGCGCGTC      <  SRR4302533.4922940/94‑1 (MQ=60)
                                                                                       CAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGCGCGTCC     <  SRR4302533.4633256/94‑1 (MQ=60)
                                                                                       CAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGCGCGTCC     >  SRR4302533.4152831/1‑94 (MQ=60)
                                                                                        AGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGCGCGTCCG    >  SRR4302533.6230749/1‑94 (MQ=60)
                                                                                        AGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGCGCGTCCG    <  SRR4302533.6080079/94‑1 (MQ=60)
                                                                                          CTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGCGCGTCCGAT  >  SRR4302533.1298192/1‑94 (MQ=60)
                                                                                          CTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGCGCGTCCGAT  <  SRR4302533.1000825/94‑1 (MQ=60)
                                                                                          CTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGCGCGTCCGAT  >  SRR4302533.3257494/1‑94 (MQ=60)
                                                                                          |                                                                                             
GGCGATGATGTCACCAACCGCAAGTCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGTTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGCGCGTCCGAT  >  NC_000913/3125269‑3125452

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑