Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,351,337	T→C	T117A (ACC→GCC)	yhcC ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,351,337	0	T	C	100.0%	777.1 / NA	244	T117A (ACC→GCC)	yhcC	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (126/118); total (126/118)

Only 100 of 251 total aligned reads displayed.

GTAGCCCCAGCTCCAGCCACACTTCGTAACCCTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGTACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCACCTGAACTTCCGCAAAGGTGCTGGTAT  >  NC_000913/3351247‑3351426

ttAGCCCCAGCTCCAGCCACACTTCGTAACCCTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCAc                                                                                         <  1:5128597/92‑1 (MQ=255)

gTAGCCCCAGCTCCAGCCACACTTCGTAACCCTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCAc                                                                                         <  1:4889688/93‑1 (MQ=255)

gTAGCCCCAGCTCCAGCCACACTTCGTAACCCTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCAc                                                                                         <  2:2851120/93‑1 (MQ=255)

  aGCCCCAGCTCCAGCCACACTTCGTAACCCTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCAccc                                                                                       <  2:5995198/93‑1 (MQ=255)

  aGCCCCAGCTCCAGCCACACTTCGTAACCCTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCAccc                                                                                       >  1:1814496/1‑93 (MQ=255)

    ccccAGCTCCAGCCACACTTCGTAACCCTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCcac                                                                                     >  2:1443363/1‑93 (MQ=255)

      ccAGCTCCAGCCACACTTCGTAACCCTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCcacac                                                                                   >  1:2480869/1‑93 (MQ=255)

      ccAGCTCCAGCCACACTTCGTAACCCTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCcacac                                                                                   <  1:5542344/93‑1 (MQ=255)

           tCCAGCCACACTTCGTAACCCTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACaaa                                                                              >  1:3758723/1‑93 (MQ=255)

            ccAGCCACACTTCGTAACCCTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAAc                                                                             >  2:17741/1‑93 (MQ=255)

             cAGCCACACTTCGTAACCCTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAAcc                                                                            >  2:3229680/1‑93 (MQ=255)

             cAGCCACACTTCGTAACCCTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAAcc                                                                            <  1:5452764/93‑1 (MQ=255)

               gCCACACTTCGTAACCCTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGa                                                                          <  2:6086494/93‑1 (MQ=255)

                  acacTTCGTAACCCTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACaa                                                                       >  1:5257293/1‑93 (MQ=255)

                  acacTTCGTAACCCTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACaa                                                                       <  1:4323023/93‑1 (MQ=255)

                  acacTTCGTAACCCTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACaa                                                                       >  1:3489101/1‑93 (MQ=255)

                    acTTCGTAACCCTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAata                                                                     >  2:66376/1‑93 (MQ=255)

                    acTTCGTAACCCTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAata                                                                     >  2:2087270/1‑93 (MQ=255)

                     cTTCGTAACCCTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAatat                                                                    <  1:803502/93‑1 (MQ=255)

                     cTTCGTAACCCTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAatat                                                                    <  1:2375151/93‑1 (MQ=255)

                      ttCGTAACCCTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATAtt                                                                   <  2:4996182/93‑1 (MQ=255)

                      ttCGTAACCCTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATAtt                                                                   <  2:1573709/93‑1 (MQ=255)

                       tCGTAACCCTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTg                                                                  >  2:3953600/1‑93 (MQ=255)

                       tCGTAACCCTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTg                                                                  <  1:5963873/93‑1 (MQ=255)

                       tCGTAACCCTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTg                                                                  <  1:5332552/93‑1 (MQ=255)

                       tCGTAACCCTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTg                                                                  <  2:4253351/93‑1 (MQ=255)

                        cGTAACCCTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTgg                                                                 <  1:2684633/93‑1 (MQ=255)

                         ctAACCCTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGc                                                                <  1:4259297/92‑1 (MQ=255)

                          gaaCCCTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCAGCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGcc                                                               <  2:872518/92‑1 (MQ=255)

                           aaCCCTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCt                                                              <  2:1308707/93‑1 (MQ=255)

                             cccTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTgg                                                            <  2:1651538/93‑1 (MQ=255)

                              ccTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGc                                                           >  1:4613228/1‑93 (MQ=255)

                               cTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCt                                                          >  1:1068448/1‑93 (MQ=255)

                                tGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTc                                                         <  2:4459565/93‑1 (MQ=255)

                                tGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTc                                                         >  2:4713494/1‑93 (MQ=255)

                                  gTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCAc                                                       >  2:1827346/1‑93 (MQ=255)

                                  gTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCAc                                                       >  1:6293312/1‑93 (MQ=255)

                                   tCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCAcc                                                      <  1:5638140/93‑1 (MQ=255)

                                    ccTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCg                                                     >  1:1116440/1‑93 (MQ=255)

                                    ccTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCg                                                     >  2:390830/1‑93 (MQ=255)

                                    ccTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCg                                                     <  2:4437839/93‑1 (MQ=255)

                                      ttATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGcc                                                   >  2:3818578/1‑93 (MQ=255)

                                       tataTTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCct                                                  >  1:204992/1‑93 (MQ=255)

                                           ttCGCAAAGCAGATCCAGCACCGCATACGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCctgctg                                              >  2:4872735/1‑93 (MQ=255)

                                             cGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGAt                                            >  2:4923692/1‑93 (MQ=255)

                                             cGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGAt                                            <  1:783709/93‑1 (MQ=255)

                                              gCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATa                                           >  2:2576358/1‑93 (MQ=255)

                                              gCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATa                                           <  1:2739240/93‑1 (MQ=255)

                                              gCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATa                                           <  1:5752269/93‑1 (MQ=255)

                                              gCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATa                                           >  1:1273971/1‑93 (MQ=255)

                                                aaaGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACa                                         <  1:2251188/93‑1 (MQ=255)

                                                aaaGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACa                                         >  2:6267166/1‑93 (MQ=255)

                                                  aGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATa                                       <  2:4082987/93‑1 (MQ=255)

                                                   gCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAg                                      <  1:2324587/93‑1 (MQ=255)

                                                     aGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGaa                                    >  1:132175/1‑93 (MQ=255)

                                                       aTCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACg                                  >  1:6025577/1‑93 (MQ=255)

                                                        tCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACgc                                 >  2:365660/1‑93 (MQ=255)

                                                         ccAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACgca                                <  1:4569164/93‑1 (MQ=255)

                                                          cAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACgcag                               <  1:3954022/93‑1 (MQ=255)

                                                          cAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACgcag                               >  2:193225/1‑93 (MQ=255)

                                                            gCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACgcagca                             >  1:6005754/1‑93 (MQ=255)

                                                            gCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACgcagca                             >  2:5479359/1‑93 (MQ=255)

                                                            gCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACgcagca                             >  2:1049767/1‑93 (MQ=255)

                                                            gCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCCGGCTCACCGCCTGCTGATACATAGAACgcagca                             >  1:2522123/1‑93 (MQ=255)

                                                             cACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCAc                            >  2:2684450/1‑93 (MQ=255)

                                                              cccGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCAcc                           <  1:3994948/92‑1 (MQ=255)

                                                              cccGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCAcc                           <  1:3193629/92‑1 (MQ=255)

                                                              aCCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCAcc                           <  1:1136771/93‑1 (MQ=255)

                                                              aCCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCAcc                           >  1:4069448/1‑93 (MQ=255)

                                                              aCCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCAcc                           >  2:5735281/1‑93 (MQ=255)

                                                               ccGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCACCt                          <  2:2896731/93‑1 (MQ=255)

                                                               ccGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCACCt                          <  2:3556730/93‑1 (MQ=255)

                                                               ccGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCACCt                          <  2:2387894/93‑1 (MQ=255)

                                                               ccGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCACCt                          >  2:2168136/1‑93 (MQ=255)

                                                                cGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCACCTg                         >  1:1013846/1‑93 (MQ=255)

                                                                  cATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCACCTGaa                       >  2:3773751/1‑93 (MQ=255)

                                                                  cATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCACCTGaa                       >  2:1218335/1‑93 (MQ=255)

                                                                   aTCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCACCTGAAc                      <  2:5386279/93‑1 (MQ=255)

                                                                   aTCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCACCTGAAc                      <  1:661171/93‑1 (MQ=255)

                                                                   aTCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCACCTGAAc                      <  1:3033743/93‑1 (MQ=255)

                                                                    tCCGGCACGCAGTCGGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCACCTGAACt                     <  2:1118433/93‑1 (MQ=255)

                                                                    cccGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCACCTGAACt                     <  2:4316172/92‑1 (MQ=255)

                                                                      cGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCACCTGAACTTc                   <  1:4939305/93‑1 (MQ=255)

                                                                      cGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCACCTGAACTTc                   >  1:2195740/1‑93 (MQ=255)

                                                                       ggCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCACCTGAACTTcc                  <  2:2457634/93‑1 (MQ=255)

                                                                       ggCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCACCTGAACTTcc                  <  2:4830795/93‑1 (MQ=255)

                                                                           cGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCACCTGAACTTCCGCaa              <  1:1983973/93‑1 (MQ=255)

                                                                            gCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCACCTGAACTTCCGCaaa             >  2:5877889/1‑93 (MQ=255)

                                                                            gCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCACCTGAACTTCCGCaaa             <  1:4367390/93‑1 (MQ=255)

                                                                             cAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCACCTGAACTTCCGCAAAg            <  2:3200663/93‑1 (MQ=255)

                                                                               gTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCACCTGAACTTCCGCAAAGGt          <  2:4632203/93‑1 (MQ=255)

                                                                               gTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCACCTGAACTTCCGCAAAGGt          >  1:5167108/1‑93 (MQ=255)

                                                                                gccGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCACCTGAACTTCCGCAAAGGTg         <  2:4542407/92‑1 (MQ=255)

                                                                                  cgggcgggCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCACCTGAACTTCCGCAAAGGTGCt       >  2:2226248/1‑93 (MQ=255)

                                                                                   gggcgggcACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCACCTGAACTTCCGCAAAGGTGCTg      >  1:1538016/1‑93 (MQ=255)

                                                                                     gcgggCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCACCTGAACTTCCGCAAAGGTGCTGGt    >  1:1662575/1‑93 (MQ=255)

                                                                                      cgggCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCACCTGAACTTCCGCAAAGGTGCTGGta   <  1:5935385/93‑1 (MQ=255)

                                                                                      cgggCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCACCTGAACTTCCGCAAAGGTGCTGGta   <  2:361778/93‑1 (MQ=255)

                                                                                       gggCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCACCTGAACTTCCGCAAAGGTGCTGGtat  >  2:5519201/1‑93 (MQ=255)

                                                                                       gggCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCACCTGAACTTCCGCAAAGGTGCTGGtat  >  1:1946925/1‑93 (MQ=255)

GTAGCCCCAGCTCCAGCCACACTTCGTAACCCTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGTACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCACCTGAACTTCCGCAAAGGTGCTGGTAT  >  NC_000913/3351247‑3351426

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TTGTAGCCCCAGCTCCAGCCACACTTCGTAACCCTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGTACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCACCTGAACTTCCGCAAAGGTGCTGGTATACG  >  NC_000913/3351245‑3351429

TTGTAGCCCCAGCTCCAGCCACACTTCGTAACCCTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCA                                                                                             >  SRR4302533.2362416/1‑94 (MQ=60)

TTGTAGCCCCAGCTCCAGCCACACTTCGTAACCCTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCA                                                                                             <  SRR4302533.1302740/94‑1 (MQ=60)

  TTAGCCCCAGCTCCAGCCACACTTCGTAACCCTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACC                                                                                           <  SRR4302533.5159579/94‑1 (MQ=60)

  GTAGCCCCAGCTCCAGCCACACTTCGTAACCCTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACC                                                                                           <  SRR4302533.4919317/94‑1 (MQ=60)

   TAGCCCCAGCTCCAGCCACACTTCGTAACCCTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCC                                                                                          >  SRR4302533.1825573/1‑94 (MQ=60)

    AGCCCCAGCTCCAGCCACACTTCGTAACCCTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCA                                                                                         >  SRR4302533.2240472/1‑94 (MQ=60)

    AGCCCCAGCTCCAGCCACACTTCGTAACCCTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCA                                                                                         <  SRR4302533.706011/94‑1 (MQ=60)

      CCCCAGCTCCAGCCACACTTCGTAACCCTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACA                                                                                       <  SRR4302533.567157/94‑1 (MQ=60)

       CCCAGCTCCAGCCACACTTCGTAACCCTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACAC                                                                                      >  SRR4302533.2496168/1‑94 (MQ=60)

       CCCAGCTCCAGCCACACTTCGTAACCCTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACAC                                                                                      >  SRR4302533.1731933/1‑94 (MQ=60)

        CCAGCTCCAGCCACACTTCGTAACCCTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACA                                                                                     >  SRR4302533.2734664/1‑94 (MQ=60)

        CCAGCTCCAGCCACACTTCGTAACCCTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACA                                                                                     <  SRR4302533.5575818/94‑1 (MQ=60)

        CCAGCTCCAGCCACACTTCGTAACCCTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACA                                                                                     >  SRR4302533.6346968/1‑94 (MQ=60)

        CCAGCTCCAGCCACACTTCGTAACCCTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACA                                                                                     <  SRR4302533.1800512/94‑1 (MQ=60)

         CAGCTCCAGCCACACTTCGTAACCCTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACAC                                                                                    >  SRR4302533.3480503/1‑94 (MQ=60)

         CAGCTCCAGCCACACTTCGTAACCCTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACAC                                                                                    >  SRR4302533.2081975/1‑94 (MQ=60)

          AGCTCCAGCCACACTTCGTAACCCTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACA                                                                                   >  SRR4302533.1030374/1‑94 (MQ=60)

          AGCTCCAGCCACACTTCGTAACCCTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACA                                                                                   >  SRR4302533.4392506/1‑94 (MQ=60)

          AGCTCCAGCCACACTTCGTAACCCTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACA                                                                                   >  SRR4302533.491505/1‑94 (MQ=60)

           GCTCCAGCCACACTTCGTAACCCTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAA                                                                                  <  SRR4302533.1225510/94‑1 (MQ=60)

           GCTCCAGCCACACTTCGTAACCCTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAA                                                                                  <  SRR4302533.973667/94‑1 (MQ=60)

           GCTCCAGCCACACTTCGTAACCCTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAA                                                                                  >  SRR4302533.603969/1‑94 (MQ=60)

            CTCCAGCCACACTTCGTAACCCTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAA                                                                                 >  SRR4302533.6015713/1‑94 (MQ=60)

            CTCCAGCCACACTTCGTAACCCTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAA                                                                                 >  SRR4302533.3781764/1‑94 (MQ=60)

             TCCAGCCACACTTCGTAACCCTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACCAAC                                                                                >  SRR4302533.2378334/1‑94 (MQ=60)

               CAGCCACACTTCGTAACCCTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCG                                                                              <  SRR4302533.5485608/94‑1 (MQ=60)

                AGCCACACTTCGTAACCCTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGA                                                                             >  SRR4302533.3977885/1‑94 (MQ=60)

                AGCCACACTTCGTAACCCTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGA                                                                             >  SRR4302533.5143948/1‑94 (MQ=60)

                 GCCACACTTCGTAACCCTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGAC                                                                            <  SRR4302533.397638/94‑1 (MQ=60)

                 GCCACACTTCGTAACCCTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGAC                                                                            >  SRR4302533.55628/1‑94 (MQ=60)

                   CACACTTCGTAACCCTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAA                                                                          >  SRR4302533.5288864/1‑94 (MQ=60)

                   CACACTTCGTAACCCTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAA                                                                          >  SRR4302533.3510619/1‑94 (MQ=60)

                    ACACTTCGTAACCCTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAAT                                                                         <  SRR4302533.4349057/94‑1 (MQ=60)

                    ACACTTCGTAACCCTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAAT                                                                         >  SRR4302533.6143367/1‑94 (MQ=60)

                      CCTTCGTAACCCTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATAT                                                                       <  SRR4302533.2559785/94‑1 (MQ=60)

                      ACTTCGTAACCCTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATAT                                                                       >  SRR4302533.194110/1‑94 (MQ=60)

                      ACTTCGTAACCCTGGTCCTTATATACGCGCAGCAAATCCAACACAGCCTCCGGCACGCAGTCCGAACGAGCACCCACACACCAACCGACAATAT                                                                       <  SRR4302533.6347084/94‑1 (MQ=23)

                       CTTCGTAACCCTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATT                                                                      <  SRR4302533.983743/94‑1 (MQ=60)

                       CTTCGTAACCCTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATT                                                                      <  SRR4302533.4548941/94‑1 (MQ=60)

                       CTTCGTAACCCTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATT                                                                      <  SRR4302533.808221/94‑1 (MQ=60)

                       CTTCGTAACCCTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATT                                                                      <  SRR4302533.2389848/94‑1 (MQ=60)

                         TCGTAACCCTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGG                                                                    <  SRR4302533.5281018/94‑1 (MQ=60)

                         TCGTAACCCTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGG                                                                    <  SRR4302533.5364511/94‑1 (MQ=60)

                         TCGTAACCCTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGG                                                                    <  SRR4302533.5999957/94‑1 (MQ=60)

                          CGTAACCCTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGC                                                                   >  SRR4302533.5665837/1‑94 (MQ=60)

                          CGTAACCCTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGC                                                                   <  SRR4302533.2701084/94‑1 (MQ=60)

                           CTAACCCTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCC                                                                  <  SRR4302533.4284966/94‑1 (MQ=60)

                             AACCCTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTG                                                                >  SRR4302533.2914541/1‑94 (MQ=60)

                             AACCCTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTG                                                                >  SRR4302533.4947708/1‑94 (MQ=60)

                               CCCTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGC                                                              >  SRR4302533.4641173/1‑94 (MQ=60)

                                CCTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCT                                                             >  SRR4302533.1074739/1‑94 (MQ=60)

                                CCTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCT                                                             >  SRR4302533.2002582/1‑94 (MQ=60)

                                 CTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTC                                                            >  SRR4302533.4016703/1‑94 (MQ=60)

                                  TGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCA                                                           >  SRR4302533.2284479/1‑94 (MQ=60)

                                   GGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCAC                                                          >  SRR4302533.6330830/1‑94 (MQ=60)

                                     TCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCG                                                        <  SRR4302533.4528287/94‑1 (MQ=60)

                                     TCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCG                                                        <  SRR4302533.5672229/94‑1 (MQ=60)

                                     TCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCG                                                        >  SRR4302533.1123065/1‑94 (MQ=60)

                                      CCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGC                                                       >  SRR4302533.5159161/1‑94 (MQ=60)

                                      CCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGC                                                       >  SRR4302533.154926/1‑94 (MQ=60)

                                       CTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCC                                                      >  SRR4302533.2464115/1‑94 (MQ=60)

                                        TTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCT                                                     >  SRR4302533.206295/1‑94 (MQ=60)

                                         TATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTG                                                    <  SRR4302533.2507937/94‑1 (MQ=60)

                                           TATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCT                                                  <  SRR4302533.5992881/94‑1 (MQ=60)

                                           TATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCT                                                  >  SRR4302533.1087267/1‑94 (MQ=60)

                                           TATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCT                                                  >  SRR4302533.4913952/1‑94 (MQ=60)

                                               CGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATA                                              <  SRR4302533.788319/94‑1 (MQ=60)

                                               CGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATA                                              >  SRR4302533.1281627/1‑94 (MQ=60)

                                               CGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATA                                              >  SRR4302533.2321208/1‑94 (MQ=60)

                                                GCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATAC                                             <  SRR4302533.5786974/94‑1 (MQ=60)

                                                GCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATAC                                             <  SRR4302533.2756037/94‑1 (MQ=60)

                                                  AAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACAT                                           >  SRR4302533.1100725/1‑94 (MQ=60)

                                                  AAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACAT                                           <  SRR4302533.2265103/94‑1 (MQ=60)

                                                   AAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATA                                          >  SRR4302533.6045584/1‑94 (MQ=60)

                                                     GCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGA                                        <  SRR4302533.2338978/94‑1 (MQ=60)

                                                      CAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAA                                       <  SRR4302533.793767/94‑1 (MQ=60)

                                                      CAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAA                                       >  SRR4302533.133006/1‑94 (MQ=60)

                                                       AGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAAC                                      <  SRR4302533.114007/94‑1 (MQ=60)

                                                       AGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACACCCTGCTGATACATATAAC                                      >  SRR4302533.4567582/1‑94 (MQ=60)

                                                        GATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACG                                     >  SRR4302533.6062089/1‑94 (MQ=60)

                                                          TCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCA                                   <  SRR4302533.59739/94‑1 (MQ=60)

                                                           CCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAG                                  <  SRR4302533.4596855/94‑1 (MQ=60)

                                                           CCAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAG                                  <  SRR4302533.2481259/94‑1 (MQ=60)

                                                            CAGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGC                                 <  SRR4302533.3978129/94‑1 (MQ=60)

                                                             AGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCA                                <  SRR4302533.745084/94‑1 (MQ=60)

                                                             AGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCA                                >  SRR4302533.6042143/1‑94 (MQ=60)

                                                             AGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCA                                >  SRR4302533.2196864/1‑94 (MQ=60)

                                                             AGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCA                                <  SRR4302533.1467878/94‑1 (MQ=60)

                                                             AGCACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCCGGCTCACCGCCTGCTGATACATAGAACGCAGCA                                >  SRR4302533.2537664/1‑94 (MQ=60)

                                                               CACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCACC                              >  SRR4302533.4094221/1‑94 (MQ=60)

                                                                CCCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCACCT                             <  SRR4302533.4317453/94‑1 (MQ=60)

                                                                CCCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCACCT                             <  SRR4302533.3213275/94‑1 (MQ=60)

                                                                CCCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCACCT                             <  SRR4302533.4019272/94‑1 (MQ=60)

                                                                ACCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCACCT                             <  SRR4302533.1143530/94‑1 (MQ=60)

                                                                 CCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCACCTG                            >  SRR4302533.5629442/1‑94 (MQ=60)

                                                                 CCGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCACCTG                            >  SRR4302533.1019782/1‑94 (MQ=60)

                                                                  CGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCACCTGA                           >  SRR4302533.2538616/1‑94 (MQ=60)

                                                                  CGCATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCACCTGA                           >  SRR4302533.6202794/1‑94 (MQ=60)

                                                                     CTCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCACCTGAACT                        <  SRR4302533.5160834/94‑1 (MQ=60)

                                                                     ATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCACCTGAACT                        <  SRR4302533.3052304/94‑1 (MQ=60)

                                                                     ATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCACCTGAACT                        <  SRR4302533.665072/94‑1 (MQ=60)

                                                                     ATCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCACCTGAACT                        >  SRR4302533.361310/1‑94 (MQ=60)

                                                                      TCCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCACCTGAACTT                       <  SRR4302533.5840797/94‑1 (MQ=60)

                                                                       CCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCACCTGAACTTC                      <  SRR4302533.429031/94‑1 (MQ=60)

                                                                       CCGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCACCTGAACTTC                      >  SRR4302533.2209270/1‑94 (MQ=60)

                                                                        CGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCACCTGAACTTCC                     >  SRR4302533.3190547/1‑94 (MQ=60)

                                                                        CGGCACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCACCTGAACTTCC                     <  SRR4302533.4969206/94‑1 (MQ=60)

                                                                         GGCACGCAGTCCGGGCGGGCACCGACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCACCTGAACTTCCG                    <  SRR4302533.4071869/94‑1 (MQ=60)

                                                                           CACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCACCTGAACTTCCGCA                  >  SRR4302533.5202382/1‑94 (MQ=60)

                                                                           CACGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCACCTGAACTTCCGCA                  >  SRR4302533.4065173/1‑94 (MQ=60)

                                                                             CGCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCACCTGAACTTCCGCAAA                <  SRR4302533.1996113/94‑1 (MQ=60)

                                                                              GCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCACCTGAACTTCCGCAAAG               <  SRR4302533.4274024/94‑1 (MQ=60)

                                                                              GCAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCACCTGAACTTCCGCAAAG               <  SRR4302533.4393755/94‑1 (MQ=60)

                                                                               CAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATTCATAGAACGCAGCACCTGAACTTCCGCAAAGG              <  SRR4302533.2968751/94‑1 (MQ=60)

                                                                               CAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCACCTGAACTTCCGCAAAGG              <  SRR4302533.1994065/94‑1 (MQ=60)

                                                                               CAGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCACCTGAACTTCCGCAAAGG              <  SRR4302533.3311418/94‑1 (MQ=60)

                                                                                AGTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCACCTGAACTTCCGCAAAGGT             >  SRR4302533.5198304/1‑94 (MQ=60)

                                                                                 GTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCACCTGAACTTCCGCAAAGGTG            <  SRR4302533.1149940/94‑1 (MQ=60)

                                                                                 GTCCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCACCTGAACTTCCGCAAAGGTG            >  SRR4302533.5339375/1‑94 (MQ=60)

                                                                                  TCCGGGCGGGCACGCACACACAAACCGACAATATTGGCCCGGCTCACCCCCTGCTGAAACATAGAACGCAGCACCTGAACTTCCGCAAAGGTGC           <  SRR4302533.1993064/94‑1 (MQ=60)

                                                                                   CCGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCACCTGAACTTCCGCAAAGGTGCT          <  SRR4302533.140802/94‑1 (MQ=60)

                                                                                    CGGGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCACCTGAACTTCCGCAAAGGTGCTG         >  SRR4302533.1547255/1‑94 (MQ=60)

                                                                                      GGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCACCTGAACTTCCGCAAAGGTGCTGGT       >  SRR4302533.1672625/1‑94 (MQ=60)

                                                                                      GGCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCACCTGAACTTCCGCAAAGGTGCTGGT       <  SRR4302533.4605561/94‑1 (MQ=60)

                                                                                       GCGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCACCTGAACTTCCGCAAAGGTGCTGGTA      >  SRR4302533.5411972/1‑94 (MQ=60)

                                                                                        CGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCACCTGAACTTCCGCAAAGGTGCTGGTAT     >  SRR4302533.1958796/1‑94 (MQ=60)

                                                                                        CGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCACCTGAACTTCCGCAAAGGTGCTGGTAT     <  SRR4302533.5971267/94‑1 (MQ=60)

                                                                                        CGGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCACCTGAACTTCCGCAAAGGTGCTGGTAT     <  SRR4302533.5420293/94‑1 (MQ=60)

                                                                                         GGGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCACCTGAACTTCCGCAAAGGTGCTGGTATA    >  SRR4302533.3624077/1‑94 (MQ=60)

                                                                                          GGCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCACCTGAACTTCCGCAAAGGTGCTGGTATAC   <  SRR4302533.2518845/94‑1 (MQ=60)

                                                                                           GCACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATATATAGAACGCAGCACCTGAACTTCCGCAAAGGTGCTGGTATACG  <  SRR4302533.3407473/94‑1 (MQ=60)

TTGTAGCCCCAGCTCCAGCCACACTTCGTAACCCTGGTCCTTATATTCGCAAAGCAGATCCAGCACCGCATCCGGCACGCAGTCCGGGCGGGTACCCACACACAAACCGACAATATTGGCCTGGCTCACCGCCTGCTGATACATAGAACGCAGCACCTGAACTTCCGCAAAGGTGCTGGTATACG  >  NC_000913/3351245‑3351429

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`