Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,353,950 A→G T562A (ACC→GCC)  gltB → glutamate synthase large subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,353,9500AG100.0% 661.0 / NA 206T562A (ACC→GCC) gltBglutamate synthase large subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (116/90);  total (116/90)

Only 100 of 229 total aligned reads displayed.

ATTATTTACGACTACTTCCGCCAGCAGTTTGCCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGCCGATTCTGCTCTACTCC  >  NC_000913/3353860‑3354039
                                                                                          |                                                                                         
 ttattTACGACTACTTCCGCCAGCAGTTTGCCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGccgcca                                                                                        >  2:2849665/1‑93 (MQ=255)
 ttattTACGACTACTTCCGCCAGCAGTTTGCCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGccgcca                                                                                        >  1:2709008/1‑93 (MQ=255)
 ttattTACGACTACTTCCGCCAGCAGTTTCCCCAGGTGACTACCCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGccgcca                                                                                        <  1:2899380/93‑1 (MQ=255)
  tattTACGACTACTTCCGCCAGCAGTTTGCCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAg                                                                                       >  1:1283702/1‑93 (MQ=255)
  tattTACGACTACTTCCGCCAGCAGTTTGCCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAg                                                                                       >  1:5155247/1‑93 (MQ=255)
    tttACGACTACTTCCGCCAGCAGTTTGCCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTa                                                                                     <  1:752420/93‑1 (MQ=255)
    tttACGACTACTCCCGCCAGCAGTTTGCCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTa                                                                                     <  1:552308/93‑1 (MQ=255)
     ttACGACTACTTCCGCCAGCAGTTTGCCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTAt                                                                                    >  2:5225124/1‑93 (MQ=255)
      tACGACTCCTCCCGCCAGCAGTTTGCCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATc                                                                                   <  1:4592323/93‑1 (MQ=255)
      tACGACTACTTCCGCCAGCAGTTTGCCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATc                                                                                   <  2:1635408/93‑1 (MQ=255)
         gACTACTTCCGCCAGCAGTTTGCCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGt                                                                                >  2:135351/1‑93 (MQ=255)
         gACTACTTCCGCCAGCAGTTTGCCCAGGGGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCggc                                                                                >  1:3139474/1‑92 (MQ=255)
          actactTCCGCCAGCAGTTTGCCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTc                                                                               <  2:1632074/93‑1 (MQ=255)
            tactacCGCCAGCAGTTTGCCAAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGt                                                                             <  2:4051308/93‑1 (MQ=255)
             actTCCGCCAGCAGTTTGCCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTg                                                                            >  2:2828816/1‑93 (MQ=255)
              ctTCCGCCAGCAGTTTGCCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGa                                                                           >  2:6104359/1‑93 (MQ=255)
                   gCCAGCAGTTTGCCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGa                                                                      >  1:672415/1‑93 (MQ=255)
                   gCCAGCAGTTTGCCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGa                                                                      >  1:1684283/1‑93 (MQ=255)
                    ccAGCAGTTTGCCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGCCGTGAAATGaa                                                                     >  1:1943073/1‑93 (MQ=255)
                     cagcagTTTGCCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAAc                                                                    <  1:2358196/93‑1 (MQ=255)
                     cagcagTTTGCCCAGGTGACAACCCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAAc                                                                    <  2:6195335/93‑1 (MQ=255)
                      agcagTTTGCCCAGGTGACTACCCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACg                                                                   <  2:5319327/93‑1 (MQ=255)
                      agcagTTTGCCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACg                                                                   >  2:789388/1‑93 (MQ=255)
                           tttGCCCAGGTGACAACCCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCttt                                                              <  1:3712092/93‑1 (MQ=255)
                            ttGCCTAGGTGACTAGCCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCtttt                                                             <  2:2129490/93‑1 (MQ=255)
                              gCCCAGGTGACTACCCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGc                                                           <  1:298200/93‑1 (MQ=255)
                                ccAGGTGACTACCCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGa                                                         <  2:1155543/93‑1 (MQ=255)
                                ccAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGa                                                         >  1:5885502/1‑93 (MQ=255)
                                ccAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGa                                                         >  1:4798521/1‑93 (MQ=255)
                                  aGGTCACTACCCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAg                                                       <  2:147862/93‑1 (MQ=255)
                                   ggTGACTACCCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGc                                                      <  2:2493391/93‑1 (MQ=255)
                                   ggTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGc                                                      >  2:5686067/1‑93 (MQ=255)
                                    gTGACTACCCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCa                                                     <  1:4025011/93‑1 (MQ=255)
                                    gTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCa                                                     >  2:1930357/1‑93 (MQ=255)
                                     tGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCag                                                    >  2:4885004/1‑93 (MQ=255)
                                     ggACTATCCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCag                                                    <  1:5095854/92‑1 (MQ=255)
                                     ggACTACCCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCag                                                    <  2:2664169/92‑1 (MQ=255)
                                     gcaCTACCCCGCCAATCGACCCGCTGCGTGAAGCGCAGGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTGGCGAAGCag                                                    <  1:1016994/91‑1 (MQ=255)
                                      gACTACCCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCaga                                                   <  1:4058788/93‑1 (MQ=255)
                                       aCTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCagag                                                  >  1:5650510/1‑93 (MQ=255)
                                          aaCCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGc                                               <  1:2033887/93‑1 (MQ=255)
                                          aaCCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGc                                               >  1:4476853/1‑93 (MQ=255)
                                           aCCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGcc                                              >  1:3903772/1‑93 (MQ=255)
                                           aCCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGcc                                              <  1:155698/93‑1 (MQ=255)
                                             ccGCCAATCGACCCGCTGCGTGGAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAg                                            >  2:4929553/1‑93 (MQ=255)
                                             ccGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAg                                            >  2:4118263/1‑93 (MQ=255)
                                              cgcgAATCGACCCGCGGCGTGATGCGCATGTTATGTCGCTGGCCGCGAGTATCGGTCGTGAATTGAACGTCTTTTGCGAAGCAGAGGGCCAgg                                           <  2:64177/93‑1 (MQ=255)
                                              cGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTGTGCGAAGCAGAGGGCCAgg                                           <  2:6129368/93‑1 (MQ=255)
                                               gCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGgc                                          >  1:4190985/1‑93 (MQ=255)
                                               gCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGgc                                          >  1:4479809/1‑93 (MQ=255)
                                               gCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAAAGTCTTTTGCGAAGCAGAGGGCCAGgc                                          >  1:1264936/1‑93 (MQ=255)
                                                 cAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGgcgc                                        >  2:2441066/1‑93 (MQ=255)
                                                    tCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCAcc                                     >  1:2426593/1‑93 (MQ=255)
                                                    tCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCAcc                                     >  1:4895014/1‑93 (MQ=255)
                                                     cGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCg                                    >  1:656886/1‑93 (MQ=255)
                                                     cGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCg                                    <  2:2970595/93‑1 (MQ=255)
                                                      gACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGt                                   >  2:4776339/1‑93 (MQ=255)
                                                      gACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGt                                   <  1:816072/93‑1 (MQ=255)
                                                       aCCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGtt                                  >  1:2626317/1‑93 (MQ=255)
                                                         ccGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTa                                >  1:5824666/1‑93 (MQ=255)
                                                          cGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTaa                               >  1:4676943/1‑93 (MQ=255)
                                                          cGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTaa                               >  1:6242774/1‑93 (MQ=255)
                                                          cGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTaa                               <  2:76979/93‑1 (MQ=255)
                                                           gCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAg                              >  1:5782372/1‑93 (MQ=255)
                                                            cTGCGTGTAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGc                             >  2:5984854/1‑93 (MQ=255)
                                                            cTGCGTGAAGCGCTTGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGTCGCACCGTTTAAGc                             <  2:3625849/93‑1 (MQ=255)
                                                             tGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCt                            >  1:3237062/1‑93 (MQ=255)
                                                             tGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCt                            >  1:2211852/1‑93 (MQ=255)
                                                             tGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCt                            >  1:1640997/1‑93 (MQ=255)
                                                              gCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCtt                           <  1:4557328/93‑1 (MQ=255)
                                                                 tGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTaa                        >  1:4445480/1‑93 (MQ=255)
                                                                 tGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTaa                        >  2:1128717/1‑93 (MQ=255)
                                                                 tGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTaa                        <  2:4429696/93‑1 (MQ=255)
                                                                  gAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTaaa                       <  2:3045976/93‑1 (MQ=255)
                                                                   aaGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAAt                      <  1:2507858/93‑1 (MQ=255)
                                                                    aGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATc                     <  2:6040164/93‑1 (MQ=255)
                                                                    aGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATc                     <  1:3472049/93‑1 (MQ=255)
                                                                    aGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATc                     <  2:4792222/93‑1 (MQ=255)
                                                                    aGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATc                     <  2:3926229/93‑1 (MQ=255)
                                                                    aGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATc                     <  2:1442588/93‑1 (MQ=255)
                                                                     gcgcATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCg                    >  1:5906378/1‑93 (MQ=255)
                                                                      cgcATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGc                   <  2:3675233/93‑1 (MQ=255)
                                                                       gcATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGcc                  <  1:531124/93‑1 (MQ=255)
                                                                         aTGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGCCGa                >  2:2786554/1‑93 (MQ=255)
                                                                          tGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGCCGAt               >  2:5606849/1‑93 (MQ=255)
                                                                           gTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGCCGAtt              >  1:623442/1‑93 (MQ=255)
                                                                           gTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGCCGAtt              >  1:4279284/1‑93 (MQ=255)
                                                                           gTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGCCGAtt              <  1:3443838/93‑1 (MQ=255)
                                                                            ttATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGCCGATTc             >  2:4296046/1‑93 (MQ=255)
                                                                              aTGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGCCGATTCTg           <  1:3838011/93‑1 (MQ=255)
                                                                              aTGTCGCTCGCCGCCAGTATCGGTCGTGAAATAAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGCCGATTCTg           >  1:1965322/1‑93 (MQ=255)
                                                                                 tcgctcgcCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGCCGATTCTGctc        >  1:2295802/1‑93 (MQ=255)
                                                                                  cgctcgcCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGCCGATTCTGctct       <  2:3476565/93‑1 (MQ=255)
                                                                                   gctcgcCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGCCGATTCTGCTCTa      <  1:3528650/93‑1 (MQ=255)
                                                                                    ctcgcCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGCCGATTCTGCTCTAc     >  2:5186013/1‑93 (MQ=255)
                                                                                    ctcgcCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGCCGATTCTGCTCTAc     <  1:1078408/93‑1 (MQ=255)
                                                                                      cgccgcCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGCCGATTCTGCTCTACTc   <  2:2575797/93‑1 (MQ=255)
                                                                                       gccgccAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGCCGATTCTGCTCTACTcc  <  2:1999903/93‑1 (MQ=255)
                                                                                       gccgccAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGCCGATTCTGCTCTACTcc  <  1:6174961/93‑1 (MQ=255)
                                                                                       gccgccAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGCCGATTCTGCTCTACTcc  >  1:1485536/1‑93 (MQ=255)
                                                                                          |                                                                                         
ATTATTTACGACTACTTCCGCCAGCAGTTTGCCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGCCGATTCTGCTCTACTCC  >  NC_000913/3353860‑3354039

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGCATTATTTACGACTACTTCCGCCAGCAGTTTGCCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGCCGATTCTGCTCTACTCCGATT  >  NC_000913/3353857‑3354043
                                                                                             |                                                                                             
CGCATTATTTACGACTACTTCCGCCAGCAGTTTGCCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCG                                                                                               <  SRR4302533.5368409/94‑1 (MQ=60)
CGCATTATTTACGACTACTCCCGCCAGCAGTTTGCCCAGGTGACTACCCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCG                                                                                               <  SRR4302533.5984477/94‑1 (MQ=60)
 GCATTATTTACGACTACTTCCGCCAGCAGTTTGCCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGC                                                                                              >  SRR4302533.2530956/1‑94 (MQ=60)
  CATTATTTACGACTACTTCCGCCAGCAGTTTGCCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCC                                                                                             >  SRR4302533.2870373/1‑94 (MQ=60)
   ATTATTTACGACTACTTCCGCCAGCAGTTTGCCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCA                                                                                            >  SRR4302533.2725632/1‑94 (MQ=60)
    TTATTTACGACTACTTCCGCCAGCAGTTTGCCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAG                                                                                           >  SRR4302533.5186379/1‑94 (MQ=60)
    TTATTTACGACTACTTCCGCCAGCAGTTTGCCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAG                                                                                           >  SRR4302533.1291426/1‑94 (MQ=60)
    TTATTTACGACTACTTCCGCCAGCAGTTTCCCCAGGTGACTACCCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAG                                                                                           <  SRR4302533.2917198/94‑1 (MQ=60)
      ATTTACGACTACTTCCGCCAGCAGTTTGCCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTA                                                                                         >  SRR4302533.264762/1‑94 (MQ=60)
      ATTTACGACTACTTCCGCCAGCAGTTTGCCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTA                                                                                         >  SRR4302533.5407615/1‑94 (MQ=60)
      ATTTACGACTACTTCCGCCAGAAGTTTGCCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTA                                                                                         <  SRR4302533.6324449/94‑1 (MQ=60)
       tttccacctcctccccCCAGCAGTTGGCCCAGTTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTAT                                                                                        <  SRR4302533.3502145/78‑1 (MQ=60)
       TTTCCGCCTACTTCCGCCAGCAGTTTGCCCAGTTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTAT                                                                                        <  SRR4302533.5809686/94‑1 (MQ=60)
       TTTACGACTACTTCCGCCAGCAGTTTGCCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTAT                                                                                        <  SRR4302533.756854/94‑1 (MQ=60)
       TTTACGACTACTCCCGCCAGCAGTTTGCCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTAT                                                                                        <  SRR4302533.555582/94‑1 (MQ=60)
        TTACGACTACTTCCGCCAGCAGTTTGCCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATC                                                                                       >  SRR4302533.3913262/1‑94 (MQ=60)
         TACGCCTACTTCCGCCAGGAGTTTGCGCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCG                                                                                      <  SRR4302533.2181079/94‑1 (MQ=60)
         TACGACTCCTCCCGCCAGCAGTTTGCCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCG                                                                                      <  SRR4302533.4620141/94‑1 (MQ=60)
          ACGACTACTCCCGCCAGCAGTTTGCCAGGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGG                                                                                     <  SRR4302533.4054095/94‑1 (MQ=60)
           CGACTACTTCCGCCAGCAGTTTGCCCAGGGGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGC                                                                                    >  SRR4302533.3158774/1‑94 (MQ=60)
             cctcctcCCGCCAGCAGTTTGCCCAGGTGACTACCCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCG                                                                                  <  SRR4302533.1539311/87‑1 (MQ=60)
             ACTACTCCCGCCAGCAGTTTGCCCAGTTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCG                                                                                  <  SRR4302533.1656661/94‑1 (MQ=60)
              CTACTTCCGCCAGCAGTTTGCCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGT                                                                                 >  SRR4302533.4599798/1‑94 (MQ=60)
               TACTTCCGCCAGCAGTTTGCCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTG                                                                                >  SRR4302533.699167/1‑94 (MQ=60)
               TACTTCCGCCAGCAGTTTGCCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTG                                                                                <  SRR4302533.3239136/94‑1 (MQ=60)
                ACTTCCGCCAGCAGTTTGCCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGA                                                                               >  SRR4302533.4338608/1‑94 (MQ=60)
                 CTTCCGCCAGCAGTTTGCCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAA                                                                              >  SRR4302533.1279416/1‑94 (MQ=60)
                    CCGCCAGCAGTTTGCCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGATCGTGAAATG                                                                           >  SRR4302533.6279991/1‑94 (MQ=60)
                     CGCCAGCAGTTTGCCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGA                                                                          >  SRR4302533.676386/1‑94 (MQ=60)
                     CGCCAGCAGTTTGCCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGA                                                                          >  SRR4302533.1694487/1‑94 (MQ=60)
                      GCCAGCAGTTTGCCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGCCGTGAAATGAA                                                                         >  SRR4302533.1954926/1‑94 (MQ=60)
                       CCAGCAGTTTGCCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAAC                                                                        >  SRR4302533.3153820/1‑94 (MQ=60)
                       CCAGCAGTTTGCCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAAC                                                                        >  SRR4302533.2156489/1‑94 (MQ=60)
                        CAGCAGTTTGCCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACG                                                                       <  SRR4302533.2372771/94‑1 (MQ=60)
                          GCAGTTTGCCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTC                                                                     >  SRR4302533.105701/1‑94 (MQ=60)
                            AGTTTGCCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTT                                                                   >  SRR4302533.2415587/1‑94 (MQ=60)
                            AGTTTGCCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTT                                                                   >  SRR4302533.1911622/1‑94 (MQ=60)
                            AGTTTGCCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTT                                                                   >  SRR4302533.3475346/1‑94 (MQ=60)
                            AGTTGGCCCAGGTGACTACCCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTT                                                                   <  SRR4302533.6159926/94‑1 (MQ=60)
                              TTTGCCGATGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTT                                                                 <  SRR4302533.4836808/94‑1 (MQ=60)
                              TTTGCCCAGGTGACAACCCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTT                                                                 <  SRR4302533.3734888/94‑1 (MQ=60)
                               TGGCCCGGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTG                                                                <  SRR4302533.4219065/94‑1 (MQ=60)
                                 gccagGGTGACTACCCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCG                                                              <  SRR4302533.624116/89‑1 (MQ=60)
                                 GCCCAGGTGACTACCCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCG                                                              <  SRR4302533.300025/94‑1 (MQ=60)
                                  CCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGA                                                             >  SRR4302533.5921066/1‑94 (MQ=60)
                                  CCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGA                                                             >  SRR4302533.4827587/1‑94 (MQ=60)
                                  CCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGA                                                             >  SRR4302533.1474961/1‑94 (MQ=60)
                                   CCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAA                                                            >  SRR4302533.2986267/1‑94 (MQ=60)
                                    CAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAG                                                           >  SRR4302533.1202448/1‑94 (MQ=60)
                                     AGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGC                                                          >  SRR4302533.5940526/1‑94 (MQ=60)
                                       GTGACTACCCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAG                                                        <  SRR4302533.357354/94‑1 (MQ=60)
                                       GTGACTACCCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAG                                                        <  SRR4302533.4049516/94‑1 (MQ=60)
                                       GTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAG                                                        <  SRR4302533.4792977/94‑1 (MQ=60)
                                       GTAACTACCCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAG                                                        <  SRR4302533.2397817/94‑1 (MQ=60)
                                        gcactacCCCGCCAATCGACCCGCTGCGTGAAGCGCAGGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTGGCGAAGCAGA                                                       <  SRR4302533.1022972/87‑1 (MQ=60)
                                        GGACTATCCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGA                                                       <  SRR4302533.5126645/94‑1 (MQ=60)
                                         GACTACCCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAG                                                      <  SRR4302533.4083495/94‑1 (MQ=60)
                                         GACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAG                                                      >  SRR4302533.5684666/1‑94 (MQ=60)
                                         GACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAG                                                      <  SRR4302533.4801686/94‑1 (MQ=60)
                                         GACGAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAG                                                      <  SRR4302533.4475142/94‑1 (MQ=60)
                                            TAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGC                                                   >  SRR4302533.4503935/1‑94 (MQ=60)
                                             AACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCC                                                  >  SRR4302533.6253424/1‑94 (MQ=60)
                                             AACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCC                                                  >  SRR4302533.3927614/1‑94 (MQ=60)
                                             AACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCC                                                  <  SRR4302533.2046433/94‑1 (MQ=60)
                                             AACCCGCCAATCGAACCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGTAGCAGAGGGCA                                                  >  SRR4302533.4583517/1‑94 (MQ=60)
                                              ACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCA                                                 <  SRR4302533.3949269/94‑1 (MQ=60)
                                              ACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCA                                                 <  SRR4302533.156691/94‑1 (MQ=60)
                                              ACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCA                                                 <  SRR4302533.1516661/94‑1 (MQ=60)
                                              ACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCA                                                 >  SRR4302533.3577921/1‑94 (MQ=60)
                                               CCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAG                                                <  SRR4302533.4088989/94‑1 (MQ=60)
                                                 CGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGC                                              >  SRR4302533.4506909/1‑94 (MQ=60)
                                                 CGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGC                                              >  SRR4302533.4216352/1‑94 (MQ=60)
                                                 CGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGC                                              <  SRR4302533.5103412/94‑1 (MQ=60)
                                                 CGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAAAGTCTTTTGCGAAGCAGAGGGCCAGGC                                              >  SRR4302533.1272504/1‑94 (MQ=60)
                                                   CCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGC                                            >  SRR4302533.3738119/1‑94 (MQ=60)
                                                    CAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCA                                           >  SRR4302533.1688095/1‑94 (MQ=60)
                                                      ATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACC                                         >  SRR4302533.2441597/1‑94 (MQ=60)
                                                      ATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACC                                         >  SRR4302533.4924679/1‑94 (MQ=60)
                                                       TCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCG                                        >  SRR4302533.660761/1‑94 (MQ=60)
                                                         GACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTT                                      >  SRR4302533.1994698/1‑94 (MQ=60)
                                                         GACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTT                                      >  SRR4302533.2642430/1‑94 (MQ=60)
                                                         GACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTT                                      <  SRR4302533.820856/94‑1 (MQ=60)
                                                           CCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTA                                    >  SRR4302533.5859851/1‑94 (MQ=60)
                                                           CCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTA                                    >  SRR4302533.236601/1‑94 (MQ=60)
                                                           CCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTA                                    >  SRR4302533.5905643/1‑94 (MQ=60)
                                                            CCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAA                                   >  SRR4302533.1576171/1‑94 (MQ=60)
                                                            CCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAA                                   >  SRR4302533.6280145/1‑94 (MQ=60)
                                                            CCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAA                                   <  SRR4302533.3510427/94‑1 (MQ=60)
                                                            CCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAA                                   >  SRR4302533.4705287/1‑94 (MQ=60)
                                                             CGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAG                                  >  SRR4302533.802126/1‑94 (MQ=60)
                                                             CGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAG                                  >  SRR4302533.5817294/1‑94 (MQ=60)
                                                              GCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGC                                 >  SRR4302533.824678/1‑94 (MQ=60)
                                                               CTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCT                                >  SRR4302533.1650939/1‑94 (MQ=60)
                                                               CTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCT                                >  SRR4302533.2225492/1‑94 (MQ=60)
                                                               CTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCT                                >  SRR4302533.3257009/1‑94 (MQ=60)
                                                                TGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTT                               <  SRR4302533.4562379/94‑1 (MQ=60)
                                                                 GCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTT                              <  SRR4302533.4584965/94‑1 (MQ=60)
                                                                 GCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTT                              <  SRR4302533.4340768/94‑1 (MQ=60)
                                                                 GCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTT                              <  SRR4302533.4704814/94‑1 (MQ=60)
                                                                  CGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTA                             <  SRR4302533.5266143/94‑1 (MQ=60)
                                                                   GTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAA                            >  SRR4302533.1332985/1‑94 (MQ=60)
                                                                   GTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAA                            <  SRR4302533.5286842/94‑1 (MQ=60)
                                                                   GTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAA                            >  SRR4302533.4472370/1‑94 (MQ=60)
                                                                    TGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAA                           >  SRR4302533.2258088/1‑94 (MQ=60)
                                                                    TGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAA                           <  SRR4302533.5679794/94‑1 (MQ=60)
                                                                      AAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATC                         <  SRR4302533.2523313/94‑1 (MQ=60)
                                                                       AGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCG                        <  SRR4302533.3493475/94‑1 (MQ=60)
                                                                       AGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCG                        >  SRR4302533.5942044/1‑94 (MQ=60)
                                                                        GCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGC                       <  SRR4302533.5827046/94‑1 (MQ=60)
                                                                          GCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGCCG                     <  SRR4302533.534282/94‑1 (MQ=60)
                                                                          GCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGCCG                     <  SRR4302533.5700294/94‑1 (MQ=60)
                                                                           CATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGCCGA                    >  SRR4302533.6305277/1‑94 (MQ=60)
                                                                            ATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGCCGAT                   >  SRR4302533.5997301/1‑94 (MQ=60)
                                                                             TGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGCCGATT                  <  SRR4302533.2552693/94‑1 (MQ=60)
                                                                             TGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGCCGATT                  >  SRR4302533.627104/1‑94 (MQ=60)
                                                                             TGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGCCGATT                  >  SRR4302533.4305017/1‑94 (MQ=60)
                                                                              GTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGCCGATTC                 >  SRR4302533.2731447/1‑94 (MQ=60)
                                                                              GTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGCCGATTC                 <  SRR4302533.3465108/94‑1 (MQ=60)
                                                                               TTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGCCGATTCT                >  SRR4302533.3194682/1‑94 (MQ=60)
                                                                                TATGTCGCTCGCCGCCAGTATCGGTCGTGAAATAAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGCCGATTCTG               >  SRR4302533.1977329/1‑94 (MQ=60)
                                                                                 ATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGCCGATTCTGC              <  SRR4302533.3861459/94‑1 (MQ=60)
                                                                                 ATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGCCGATTCTGC              >  SRR4302533.2449805/1‑94 (MQ=60)
                                                                                  TGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGCCGATTCTGCT             <  SRR4302533.1969159/94‑1 (MQ=60)
                                                                                   GTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGCCGATTCTGCTC            >  SRR4302533.2310019/1‑94 (MQ=60)
                                                                                      GCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGCCGATTCTGCTCTAC         <  SRR4302533.3550398/94‑1 (MQ=60)
                                                                                       CTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGCCGATTCTGCTCTACT        <  SRR4302533.1084763/94‑1 (MQ=60)
                                                                                        TCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGCCGATTCTGCTCTACTC       >  SRR4302533.3999932/1‑94 (MQ=60)
                                                                                         CGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGCCGATTCTGCTCTACTCC      >  SRR4302533.1494503/1‑94 (MQ=60)
                                                                                         CGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGCCGATTCTGCTCTACTCC      <  SRR4302533.5141459/94‑1 (MQ=60)
                                                                                         CGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGCCGATTCTGCTCTACTCC      >  SRR4302533.670738/1‑94 (MQ=60)
                                                                                         CGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGCCGATTCTGCTCTACTCC      <  SRR4302533.4506183/94‑1 (MQ=60)
                                                                                          GCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGCCGATTCTGCTCTACTCCG     >  SRR4302533.2690793/1‑94 (MQ=60)
                                                                                          GCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGCCGATTCTGCTCTACTCCG     <  SRR4302533.6212066/94‑1 (MQ=60)
                                                                                           CCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGCCGATTCTGCTCTACTCCGA    >  SRR4302533.3089942/1‑94 (MQ=60)
                                                                                           CCGCCAGTATCGGTCGTGAAATGAACGTCTTTTACGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGCCGATTCTGCTCTACTCCGA    <  SRR4302533.1075743/94‑1 (MQ=60)
                                                                                             GCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGCCGATTCTGCTCTACTCCGATT  <  SRR4302533.3740149/94‑1 (MQ=60)
                                                                                             GCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGCCGATTCTGCTCTACTCCGATT  >  SRR4302533.1024031/1‑94 (MQ=60)
                                                                                             |                                                                                             
CGCATTATTTACGACTACTTCCGCCAGCAGTTTGCCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGCCGATTCTGCTCTACTCCGATT  >  NC_000913/3353857‑3354043

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑