Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,356,616 T→C A1450A (GCT→GCC)  gltB → glutamate synthase large subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,356,6160TC100.0% 797.9 / NA 249A1450A (GCT→GCC) gltBglutamate synthase large subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (146/103);  total (146/103)

Only 100 of 256 total aligned reads displayed.

GACCGGCGGTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCTCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAAGAGATTCTGGCGAA  >  NC_000913/3356526‑3356705
                                                                                          |                                                                                         
gACCGGCGGTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGccct                                                                                         <  1:2895215/93‑1 (MQ=255)
gACCGGCGGTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGccct                                                                                         >  1:3868579/1‑93 (MQ=255)
gACCGGCGGTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGccct                                                                                         <  2:1765142/93‑1 (MQ=255)
 aCCGGCGGTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTg                                                                                        <  1:1393500/93‑1 (MQ=255)
        gTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATcc                                                                                 >  2:6080015/1‑93 (MQ=255)
          ttCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCAt                                                                               >  2:2905890/1‑93 (MQ=255)
             gCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGaa                                                                            <  2:1998181/93‑1 (MQ=255)
              cTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAag                                                                           <  1:6193634/93‑1 (MQ=255)
              cTTACGTCCTCGATGAAAGTGGCGTTTGCCGCAAACGCGTTACCCCGAAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAag                                                                           <  2:3434876/93‑1 (MQ=255)
               ttACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAaga                                                                          <  1:3127191/93‑1 (MQ=255)
                tACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAagag                                                                         >  2:2642205/1‑93 (MQ=255)
                tACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAagag                                                                         <  2:4270106/93‑1 (MQ=255)
                tACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAagag                                                                         >  1:2110071/1‑93 (MQ=255)
                 aCGTTCTCGATGAAGGCGGCGATTTCCGCAAACGCGTTAACCCGAAACTGGTCGAGGTCTGAAGCGTTGACGCCCTGGCGATCCATGAAGAGc                                                                        <  2:1561032/93‑1 (MQ=255)
                 aCGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGc                                                                        >  1:584232/1‑93 (MQ=255)
                  cGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCa                                                                       >  2:394151/1‑93 (MQ=255)
                  cGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCa                                                                       <  2:856678/93‑1 (MQ=255)
                   gTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCAt                                                                      >  1:2623813/1‑93 (MQ=255)
                   gTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCAt                                                                      >  2:598643/1‑93 (MQ=255)
                    ttCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATc                                                                     >  2:5146370/1‑93 (MQ=255)
                      ctcGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTg                                                                   >  2:5470583/1‑93 (MQ=255)
                      ctcGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTg                                                                   >  1:1183767/1‑93 (MQ=255)
                        cGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCg                                                                 >  2:2292572/1‑93 (MQ=255)
                         gATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGt                                                                >  1:6242693/1‑93 (MQ=255)
                         gATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGt                                                                >  1:5936687/1‑93 (MQ=255)
                         gATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGt                                                                >  1:5628703/1‑93 (MQ=255)
                         gATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGt                                                                <  1:2582790/93‑1 (MQ=255)
                         gATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGt                                                                >  1:1892529/1‑93 (MQ=255)
                          aTGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTg                                                               <  1:3082912/93‑1 (MQ=255)
                           tGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTgg                                                              >  1:4909057/1‑93 (MQ=255)
                           tGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTgg                                                              >  2:6288164/1‑93 (MQ=255)
                            gAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGt                                                             <  1:441870/93‑1 (MQ=255)
                            gAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGt                                                             <  2:4036797/93‑1 (MQ=255)
                              aaGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCt                                                           >  2:6017251/1‑93 (MQ=255)
                              aaGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCt                                                           >  2:1744056/1‑93 (MQ=255)
                              aaGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCt                                                           >  1:1970788/1‑93 (MQ=255)
                                gcggcgATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTa                                                         >  1:911833/1‑93 (MQ=255)
                                 cggcgATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTAt                                                        >  1:1104868/1‑93 (MQ=255)
                                  ggcgATTTCCGCAAACGCGTTACCCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATc                                                       <  1:812355/93‑1 (MQ=255)
                                  ggcgATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATc                                                       >  2:4307567/1‑93 (MQ=255)
                                   gcgATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATcc                                                      >  2:536272/1‑92 (MQ=255)
                                   gcgATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCa                                                      >  1:4675602/1‑93 (MQ=255)
                                      aTTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCg                                                   >  1:5311444/1‑93 (MQ=255)
                                      aTTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCg                                                   >  2:534548/1‑93 (MQ=255)
                                      aTTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCg                                                   >  2:5755933/1‑93 (MQ=255)
                                      aTTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGCCTTATCACCg                                                   >  2:1335729/1‑93 (MQ=255)
                                           cGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCAt                                              <  2:5116329/93‑1 (MQ=255)
                                           cGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCAt                                              >  2:2857216/1‑93 (MQ=255)
                                            gCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCAtg                                             >  2:1794430/1‑93 (MQ=255)
                                             cAAACGCGTTAACCCGGAACTGGCCGAGGTCTTACGCGTTGAGGCCCTGGCGATCCATGAAGAGCATCTGCGAGGTCTAATTACCGCCCAtgt                                            >  1:3196775/1‑93 (MQ=255)
                                              aaaCGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCAtgtg                                           >  2:248683/1‑93 (MQ=255)
                                              aaaCGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCAtgtg                                           >  1:650536/1‑93 (MQ=255)
                                                aCGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTgca                                         <  2:3232450/93‑1 (MQ=255)
                                                aCGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTgca                                         <  1:1327012/93‑1 (MQ=255)
                                                aCGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTgca                                         <  2:519674/93‑1 (MQ=255)
                                                  gcgTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTgcagc                                       >  2:6272753/1‑93 (MQ=255)
                                                   cgTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTgcagca                                      <  1:3941251/93‑1 (MQ=255)
                                                   cgTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTgcagca                                      <  1:363218/93‑1 (MQ=255)
                                                    gTTAACCCGGAACTGGTCGAGGGCTTAAGCGCTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCAt                                     >  2:6087687/1‑93 (MQ=255)
                                                     ttAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATa                                    >  1:2856858/1‑93 (MQ=255)
                                                       aaCCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATAcc                                  >  2:120297/1‑93 (MQ=255)
                                                       aaCCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATAcc                                  >  2:5657345/1‑93 (MQ=255)
                                                       aaCCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATAcc                                  <  2:2176442/93‑1 (MQ=255)
                                                         cccGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCgg                                >  2:5667315/1‑93 (MQ=255)
                                                         cccGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCgg                                <  2:3090221/93‑1 (MQ=255)
                                                          ccGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGc                               >  2:454095/1‑93 (MQ=255)
                                                          ccGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGc                               <  1:1677247/93‑1 (MQ=255)
                                                           cGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGct                              >  1:1208148/1‑93 (MQ=255)
                                                           cGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGct                              >  1:512978/1‑93 (MQ=255)
                                                            ggAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGctc                             >  2:1807345/1‑93 (MQ=255)
                                                             gAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGctct                            <  1:5821297/93‑1 (MQ=255)
                                                               aCTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCa                          >  2:3271816/1‑93 (MQ=255)
                                                                cTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAg                         <  1:1069603/93‑1 (MQ=255)
                                                                 tGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAgc                        >  2:5554316/1‑93 (MQ=255)
                                                                   gTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAgcgc                      >  1:927017/1‑93 (MQ=255)
                                                                     cGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCgg                    <  2:3561650/93‑1 (MQ=255)
                                                                     cGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCgg                    <  1:2064857/93‑1 (MQ=255)
                                                                      gAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGt                   >  2:537877/1‑93 (MQ=255)
                                                                      gAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGt                   <  2:2081538/93‑1 (MQ=255)
                                                                       aGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTg                  <  1:3373449/93‑1 (MQ=255)
                                                                       aGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTg                  >  1:1772505/1‑93 (MQ=255)
                                                                         gTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGaa                <  1:3489940/93‑1 (MQ=255)
                                                                          tCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAag               >  1:6120452/1‑93 (MQ=255)
                                                                          tCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAag               >  1:4967304/1‑93 (MQ=255)
                                                                          tCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAag               <  2:4776218/93‑1 (MQ=255)
                                                                           cTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAaga              <  1:5564131/93‑1 (MQ=255)
                                                                           cTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAaga              <  2:2336683/93‑1 (MQ=255)
                                                                            ttAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAagag             >  1:4252390/1‑93 (MQ=255)
                                                                            ttAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAagag             >  2:4487750/1‑93 (MQ=255)
                                                                             tAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAAgaga            >  2:3847999/1‑93 (MQ=255)
                                                                              aaGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAAGAGAt           >  1:1469252/1‑93 (MQ=255)
                                                                                gCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAAGAGATTc         >  1:410277/1‑93 (MQ=255)
                                                                                 cGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAAGAGATTCt        <  2:2688443/93‑1 (MQ=255)
                                                                                  gTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAAGAGATTCTg       >  2:4924525/1‑93 (MQ=255)
                                                                                   ttGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAAGAGATTCTgg      <  2:2449507/93‑1 (MQ=255)
                                                                                   ttGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAAGAGATTCTgg      >  1:703046/1‑93 (MQ=255)
                                                                                   ttGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAAGAGATTCTgg      >  2:3627644/1‑93 (MQ=255)
                                                                                    tGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAAGAGATTCTGGc     <  2:5559597/93‑1 (MQ=255)
                                                                                     gACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAAGAGATTCTGGCg    >  2:3472465/1‑93 (MQ=255)
                                                                                       cGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAAGAGATTCTGGCGaa  >  1:454109/1‑93 (MQ=255)
                                                                                          |                                                                                         
GACCGGCGGTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCTCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAAGAGATTCTGGCGAA  >  NC_000913/3356526‑3356705

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CATGACCGGCGGTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCTCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAAGAGATTCTGGCGAACTGG  >  NC_000913/3356523‑3356709
                                                                                             |                                                                                             
CATGACCGGCGGTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCC                                                                                               >  SRR4302533.2688919/1‑94 (MQ=60)
 ATGACCGGCGGTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCTGCAAAAGCGTTAACCCGGAACTGGTCGAGGTCTTCAGCGTTGACGCCC                                                                                              >  SRR4302533.3428719/1‑94 (MQ=60)
 ATGACCGGCGGTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCC                                                                                              >  SRR4302533.374801/1‑94 (MQ=60)
  TGACCGGCGGTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCT                                                                                             >  SRR4302533.3892198/1‑94 (MQ=60)
  TGACCGGCGGTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCT                                                                                             <  SRR4302533.1334882/94‑1 (MQ=60)
   GACCGGCGGTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTG                                                                                            <  SRR4302533.808533/94‑1 (MQ=60)
   GACCGGCGGTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTG                                                                                            <  SRR4302533.2913018/94‑1 (MQ=60)
    GCCGGCGGTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGG                                                                                           <  SRR4302533.6246590/94‑1 (MQ=60)
    ACCGGCGGTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGG                                                                                           <  SRR4302533.3492840/94‑1 (MQ=60)
    ACCGGCGGTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGG                                                                                           <  SRR4302533.1401896/94‑1 (MQ=60)
       GGCGGTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGA                                                                                        >  SRR4302533.697335/1‑94 (MQ=60)
        GCGGTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGAT                                                                                       >  SRR4302533.3159316/1‑94 (MQ=60)
              TCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGA                                                                                 >  SRR4302533.1184219/1‑94 (MQ=60)
              TCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGA                                                                                 <  SRR4302533.4629272/94‑1 (MQ=60)
              TCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGA                                                                                 <  SRR4302533.5749939/94‑1 (MQ=60)
               CGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAA                                                                                <  SRR4302533.617441/94‑1 (MQ=60)
                GCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAG                                                                               >  SRR4302533.171734/1‑94 (MQ=60)
                 CTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGA                                                                              <  SRR4302533.1424526/94‑1 (MQ=60)
                 CTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGA                                                                              >  SRR4302533.3870308/1‑94 (MQ=60)
                 CTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGA                                                                              <  SRR4302533.6230799/94‑1 (MQ=60)
                  TTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAG                                                                             <  SRR4302533.3146415/94‑1 (MQ=60)
                  TTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAG                                                                             >  SRR4302533.5450631/1‑94 (MQ=60)
                  TTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAG                                                                             >  SRR4302533.2123086/1‑94 (MQ=60)
                   TACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGC                                                                            >  SRR4302533.587677/1‑94 (MQ=60)
                   TACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGC                                                                            >  SRR4302533.632940/1‑94 (MQ=60)
                    TCGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCA                                                                           <  SRR4302533.4107918/94‑1 (MQ=60)
                     CGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCAT                                                                          >  SRR4302533.2639918/1‑94 (MQ=60)
                     CGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCAT                                                                          >  SRR4302533.5179213/1‑94 (MQ=60)
                       TTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCT                                                                        >  SRR4302533.6229465/1‑94 (MQ=60)
                        TCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTG                                                                       >  SRR4302533.1190873/1‑94 (MQ=60)
                           CGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGT                                                                    >  SRR4302533.1904084/1‑94 (MQ=60)
                           CGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGT                                                                    >  SRR4302533.6280064/1‑94 (MQ=60)
                           CGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGT                                                                    >  SRR4302533.5662739/1‑94 (MQ=60)
                           CGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGT                                                                    >  SRR4302533.5972583/1‑94 (MQ=60)
                            GATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTG                                                                   <  SRR4302533.2598673/94‑1 (MQ=60)
                            GATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTG                                                                   <  SRR4302533.5821032/94‑1 (MQ=60)
                             ATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGG                                                                  <  SRR4302533.1726731/94‑1 (MQ=60)
                             ATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGG                                                                  >  SRR4302533.4938797/1‑94 (MQ=60)
                             ATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGG                                                                  <  SRR4302533.3101864/94‑1 (MQ=60)
                             ATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGG                                                                  >  SRR4302533.2896997/1‑94 (MQ=60)
                             ATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCAATCCATGAAGAGtatcttcgggt                                                                  >  SRR4302533.2896996/1‑83 (MQ=60)
                              TGAAAGCGGCTATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGT                                                                 >  SRR4302533.2219071/1‑94 (MQ=60)
                               GAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTC                                                                <  SRR4302533.444513/94‑1 (MQ=60)
                                AAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCT                                                               >  SRR4302533.114568/1‑94 (MQ=60)
                                AAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCT                                                               <  SRR4302533.4500132/94‑1 (MQ=60)
                                AAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCT                                                               >  SRR4302533.1982845/1‑94 (MQ=60)
                                 AAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTT                                                              <  SRR4302533.1802702/94‑1 (MQ=60)
                                 AAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTT                                                              <  SRR4302533.2642156/94‑1 (MQ=60)
                                  AGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTA                                                             >  SRR4302533.917181/1‑94 (MQ=60)
                                   GCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTAT                                                            >  SRR4302533.1111416/1‑94 (MQ=60)
                                   GCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGAGGTCTTAT                                                            <  SRR4302533.1245046/94‑1 (MQ=60)
                                    CGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATC                                                           <  SRR4302533.1201819/94‑1 (MQ=60)
                                     GGCGATTTCCGCAAACGCGTTACCCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCA                                                          <  SRR4302533.817114/94‑1 (MQ=60)
                                     GGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCA                                                          >  SRR4302533.4703938/1‑94 (MQ=60)
                                      GCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCAC                                                         <  SRR4302533.817953/94‑1 (MQ=60)
                                       CGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACC                                                        >  SRR4302533.1384134/1‑94 (MQ=60)
                                       CGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACC                                                        <  SRR4302533.4136885/94‑1 (MQ=60)
                                       CGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACA                                                        >  SRR4302533.2073166/1‑94 (MQ=60)
                                       CGATTTCCACAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACC                                                        >  SRR4302533.592069/1‑94 (MQ=60)
                                        GATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCG                                                       >  SRR4302533.5343275/1‑94 (MQ=60)
                                          TTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAG                                                     <  SRR4302533.1500002/94‑1 (MQ=60)
                                            TCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCA                                                   >  SRR4302533.1408727/1‑94 (MQ=60)
                                            TCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCA                                                   <  SRR4302533.3107516/94‑1 (MQ=60)
                                               GCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGT                                                >  SRR4302533.4572982/1‑94 (MQ=60)
                                               GCAAACGCGTTAACCCGGAACTGGCCGAGGTCTTACGCGTTGAGGCCCTGGCGATCCATGAAGAGCATCTGCGAGGTCTaattaccgcccatgt                                                >  SRR4302533.3216446/1‑79 (MQ=60)
                                                CAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTG                                               >  SRR4302533.2055643/1‑94 (MQ=60)
                                                CAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTG                                               <  SRR4302533.1891554/94‑1 (MQ=60)
                                                CAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTG                                               >  SRR4302533.5222033/1‑94 (MQ=60)
                                                CAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTG                                               >  SRR4302533.654378/1‑94 (MQ=60)
                                                 AAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGC                                              >  SRR4302533.3056091/1‑94 (MQ=60)
                                                   ACGCGTTACCCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAG                                            <  SRR4302533.4070925/94‑1 (MQ=60)
                                                   ACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAG                                            <  SRR4302533.1334977/94‑1 (MQ=60)
                                                    CGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGC                                           <  SRR4302533.1332206/94‑1 (MQ=60)
                                                     GCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCA                                          <  SRR4302533.5870268/94‑1 (MQ=60)
                                                     GCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCA                                          >  SRR4302533.2768273/1‑94 (MQ=60)
                                                      CGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCAT                                         >  SRR4302533.3065864/1‑94 (MQ=60)
                                                      CGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCAT                                         <  SRR4302533.3965300/94‑1 (MQ=60)
                                                      CGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCAT                                         <  SRR4302533.365427/94‑1 (MQ=60)
                                                      CGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCAT                                         <  SRR4302533.2098386/94‑1 (MQ=60)
                                                       GTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATA                                        <  SRR4302533.918182/94‑1 (MQ=60)
                                                       GTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATA                                        >  SRR4302533.2874437/1‑94 (MQ=60)
                                                         TAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACC                                      <  SRR4302533.3963197/94‑1 (MQ=60)
                                                           ACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGG                                    <  SRR4302533.6290365/94‑1 (MQ=60)
                                                           ACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGG                                    >  SRR4302533.3275089/1‑94 (MQ=60)
                                                             CCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCT                                  <  SRR4302533.3277603/94‑1 (MQ=60)
                                                             CCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCT                                  >  SRR4302533.1215401/1‑94 (MQ=60)
                                                             CCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCT                                  >  SRR4302533.516026/1‑94 (MQ=60)
                                                             CCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCT                                  <  SRR4302533.1687401/94‑1 (MQ=60)
                                                               GGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCT                                >  SRR4302533.3124154/1‑94 (MQ=60)
                                                                GAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTC                               <  SRR4302533.5856460/94‑1 (MQ=60)
                                                                   CTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGC                            <  SRR4302533.1075903/94‑1 (MQ=60)
                                                                    TGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCG                           <  SRR4302533.4413522/94‑1 (MQ=60)
                                                                     GGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGC                          >  SRR4302533.932458/1‑94 (MQ=60)
                                                                        CGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGT                       <  SRR4302533.2077583/94‑1 (MQ=60)
                                                                        CGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGT                       >  SRR4302533.4729456/1‑94 (MQ=60)
                                                                         GAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTG                      >  SRR4302533.699158/1‑94 (MQ=60)
                                                                         GAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTG                      >  SRR4302533.4964144/1‑94 (MQ=60)
                                                                         GAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTG                      >  SRR4302533.1783298/1‑94 (MQ=60)
                                                                          AGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGA                     <  SRR4302533.3394283/94‑1 (MQ=60)
                                                                          AGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGA                     >  SRR4302533.3515740/1‑94 (MQ=60)
                                                                          AGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGA                     <  SRR4302533.3533587/94‑1 (MQ=60)
                                                                           GGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAA                    <  SRR4302533.593396/94‑1 (MQ=60)
                                                                            GTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAAG                   >  SRR4302533.5367149/1‑94 (MQ=60)
                                                                            GTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAAG                   >  SRR4302533.4997422/1‑94 (MQ=60)
                                                                            GTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAAG                   <  SRR4302533.3511462/94‑1 (MQ=60)
                                                                            GTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAAG                   >  SRR4302533.6038595/1‑94 (MQ=60)
                                                                            GTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAAG                   >  SRR4302533.6157359/1‑94 (MQ=60)
                                                                             TCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAAGA                  <  SRR4302533.2561402/94‑1 (MQ=60)
                                                                              CTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAAGAG                 >  SRR4302533.4278038/1‑94 (MQ=60)
                                                                              CTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAAGAG                 <  SRR4302533.1978855/94‑1 (MQ=60)
                                                                              CTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAAGAG                 <  SRR4302533.5597761/94‑1 (MQ=60)
                                                                               TTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAAGAGA                >  SRR4302533.496639/1‑94 (MQ=60)
                                                                                TAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAAGAGAT               >  SRR4302533.1478109/1‑94 (MQ=60)
                                                                                  AGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAAGAGATTC             >  SRR4302533.412736/1‑94 (MQ=60)
                                                                                  AGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAAGAGATTC             >  SRR4302533.3943679/1‑94 (MQ=60)
                                                                                  AGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAAGAGATTC             >  SRR4302533.2636907/1‑94 (MQ=60)
                                                                                  AGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAAGAGAATC             >  SRR4302533.442355/1‑94 (MQ=60)
                                                                                     GTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAAGAGATTCTGG          >  SRR4302533.5618404/1‑94 (MQ=60)
                                                                                     GTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAAGAGATTCTGG          >  SRR4302533.707203/1‑94 (MQ=60)
                                                                                     GTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAAGAGATTCTGG          >  SRR4302533.5111320/1‑94 (MQ=60)
                                                                                      TTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAAGAGATTCTGGC         <  SRR4302533.1077527/94‑1 (MQ=60)
                                                                                        GACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAAGAGATTCTGGCGA       <  SRR4302533.874684/94‑1 (MQ=60)
                                                                                         ACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAAGAGATTCTGGCGAA      >  SRR4302533.456824/1‑94 (MQ=60)
                                                                                           GCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAAGAGATTCTGGCGAACT    >  SRR4302533.2929478/1‑94 (MQ=60)
                                                                                             CCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAAGAGATTCTGGCGAACTGG  >  SRR4302533.5951086/1‑94 (MQ=60)
                                                                                             |                                                                                             
CATGACCGGCGGTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCTCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAAGAGATTCTGGCGAACTGG  >  NC_000913/3356523‑3356709

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑