Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,374,263 T→C Q50R (CAA→CGA)  sspB ← stringent starvation protein B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,374,2630TC100.0% 781.4 / NA 242Q50R (CAA→CGA) sspBstringent starvation protein B
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (118/124);  total (118/124)

Only 100 of 250 total aligned reads displayed.

GGAATGCCACCAAAGCGCGCGTTAAAGCGCACCTCATCATTCGCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTTGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGGGAGCGTCACATCCACCACCAGGTGCGGCGTGAGCTGGTTATCCAGCAAC  >  NC_000913/3374173‑3374352
                                                                                          |                                                                                         
 gAATGCCACCAAAGCGCGCGTTAAAGCGCACCTCATCATTCGCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGcc                                                                                        >  1:5815695/1‑93 (MQ=255)
 gAATGCCACCAAAGCGCGCGTTAAAGCGCACCTCATCATTCGCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGcc                                                                                        >  1:5537518/1‑93 (MQ=255)
 gAATGCCACCAAAGCGCGCGTTAAAGCGCACCTCATCATTCGCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGcc                                                                                        <  1:4815845/93‑1 (MQ=255)
 gAATGCCACCAAAGCGCGCGTTAAAGCGCACCTCATCATTCGCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGcc                                                                                        >  1:1681574/1‑93 (MQ=255)
  aaTGCCACCAAAGCGCGCGTTAAAGCGCACCTCATCATTCGCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGccc                                                                                       <  2:2540464/93‑1 (MQ=255)
     gCCACCAAAGCGCGCGTTAAAGCGCACCTCATCATTCGCCATTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTc                                                                                    <  1:150232/93‑1 (MQ=255)
       caccaAAGCGCGCGTTAAAGCGCACCTCATCATTCGCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCAc                                                                                  <  1:685186/93‑1 (MQ=255)
       caccaAAGCGCGCGTTAAAGCGCACCTCATCATTCGCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCAc                                                                                  >  2:5450273/1‑93 (MQ=255)
        accaAAGCGCGCGTTAAAGCGCACCTCATCATTCGCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCAcg                                                                                 >  2:3986188/1‑93 (MQ=255)
        accaAAGCGCGCGTTAAAGCGCACCTCATCATTCGCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCAcg                                                                                 >  2:2153320/1‑93 (MQ=255)
        accaAAGCGCGCGTTAAAGCGCACCTCATCATTCGCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCAcg                                                                                 >  1:2272440/1‑93 (MQ=255)
         ccaAAGCGCGCGTTAAAGCGCACCTCATCATTCGCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCAcgc                                                                                <  2:4082071/93‑1 (MQ=255)
         ccaAAGCGCGCGTTAAAGCGCACCTCATCATTCGCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCAcgc                                                                                <  1:2579155/93‑1 (MQ=255)
            aaGCGCGCGTTAAAGCGCACCTCATCATTCGCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCa                                                                             <  2:4856193/93‑1 (MQ=255)
             aGCGCGCGTTAAAGCGCACCTCATCATTCGCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCat                                                                            >  2:2499195/1‑93 (MQ=255)
              gcgcgcGTTAAAGCGCACCTCATCATTCGCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCata                                                                           >  1:1604855/1‑93 (MQ=255)
                gcgcgTTAAAGCGCACCTCATCATTCGCCAGTTCCAGCTTGCCGACAGCACGCGGCGCAATGTTGCGTACGATTCGCCCCTCACGCGCATAtt                                                                         >  2:1052988/1‑93 (MQ=255)
                gcgcgTTAAAGCGCACCTCATCATTCGCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATAtt                                                                         <  1:2889196/93‑1 (MQ=255)
                 cgcgTTAACGCGCACCTCATCATTCGCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTc                                                                        >  2:6128316/1‑93 (MQ=255)
                 cgcgTTAAAGCGCACCTCATCATTCGCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTc                                                                        <  2:3119367/93‑1 (MQ=255)
                  gcgTTAAAGCGCACCTCATCATTCGCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTcc                                                                       >  1:386688/1‑93 (MQ=255)
                    gTTAAAGCGCACCTCATCATTCGCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCAt                                                                     <  2:1815544/93‑1 (MQ=255)
                    gTTAAAGCGCACCTCATCATTCGCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCAt                                                                     >  1:4147478/1‑93 (MQ=255)
                     ttAAAGCTCACCTCATCATTCGCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATa                                                                    <  1:3173972/93‑1 (MQ=255)
                     ttAAAGCGCACCTCATCATTCGCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATa                                                                    <  2:5084416/93‑1 (MQ=255)
                     ttAAAGCGCACCTCATCATTCGCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATa                                                                    >  1:4414531/1‑93 (MQ=255)
                     ttAAAGCGCACCTCATCATTCGCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATa                                                                    <  1:3374005/93‑1 (MQ=255)
                      tAAAGCGCACCTCATCATTCGCCAGTTCCAGATTGCCGACAGCACTCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTACATAg                                                                   >  1:3925120/1‑93 (MQ=255)
                      tAAAGCGCACCTCATCATTCGCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAg                                                                   >  2:2851/1‑93 (MQ=255)
                      tAAAGCGCACCTCATCATTCGCCAGTTCCAGATTGCCGACAGCACGCAGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAg                                                                   <  2:3440668/93‑1 (MQ=255)
                       aaaGCGCACCTCATCATTCGCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAgg                                                                  <  2:4921292/93‑1 (MQ=255)
                          gcgcACCTCATCATTCGCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAAc                                                               >  1:3026644/1‑93 (MQ=255)
                          gcgcACCTCATCATTCGCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAAc                                                               <  2:3564554/93‑1 (MQ=255)
                           cgcACCTCATCATTCGCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAAcc                                                              <  1:1603008/93‑1 (MQ=255)
                           cgcACCTCATCATTCGCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAAcc                                                              <  2:3416793/93‑1 (MQ=255)
                            gcACCTCATCATTCGCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCt                                                             >  1:1046082/1‑93 (MQ=255)
                            gcACCTCATCATTCGCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCt                                                             >  1:1839478/1‑93 (MQ=255)
                              aCCTCATCATTCGCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGc                                                           >  1:1652137/1‑93 (MQ=255)
                                cTCATCATTCGCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCAc                                                         >  2:428956/1‑93 (MQ=255)
                                 tcatcaTTCGCCAGTTCCAGGTTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCAcc                                                        >  2:6057373/1‑92 (MQ=255)
                                   atcatTCGCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGcc                                                      >  2:1356956/1‑93 (MQ=255)
                                    tcatTCGCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCa                                                     >  2:5348880/1‑93 (MQ=255)
                                    tcatTCGCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCa                                                     <  1:2556925/93‑1 (MQ=255)
                                     catTCGCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAg                                                    >  2:3918868/1‑93 (MQ=255)
                                      atTCGCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAgg                                                   >  2:5291394/1‑93 (MQ=255)
                                        tCGCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGGGa                                                 <  1:2108427/93‑1 (MQ=255)
                                        tCGCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGGGa                                                 <  1:1884025/93‑1 (MQ=255)
                                          gCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGGGAGc                                               >  1:476758/1‑93 (MQ=255)
                                           ccAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGGGAGCg                                              >  1:3994316/1‑93 (MQ=255)
                                            cAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGGGAGCGt                                             >  2:3462681/1‑93 (MQ=255)
                                             aGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGGGAGCGTc                                            <  1:5446589/93‑1 (MQ=255)
                                              gTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGGGAGCGTca                                           >  2:1048580/1‑93 (MQ=255)
                                                tCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGGGAGCGTcaca                                         <  2:651413/93‑1 (MQ=255)
                                                 ccAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGGGAGCGTCACAt                                        >  1:1764980/1‑93 (MQ=255)
                                                 ccAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGGGAGCGTCACAt                                        <  1:6020234/93‑1 (MQ=255)
                                                   aGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGGGAGCGTCACATcc                                      <  2:3323516/93‑1 (MQ=255)
                                                   aGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGGGAGCGTCACATcc                                      <  2:5550249/93‑1 (MQ=255)
                                                   aGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGGGAGCGTCACATcc                                      <  2:5001159/93‑1 (MQ=255)
                                                     aTTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGGGAGCGTCACATccac                                    <  1:62840/93‑1 (MQ=255)
                                                        gCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGGGAGCGTCACATccaccac                                 <  2:4044436/93‑1 (MQ=255)
                                                        gCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGGGAGCGTCACATccaccac                                 <  2:3683403/93‑1 (MQ=255)
                                                        gCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGGGAGCGTCACATccaccac                                 <  1:2832002/93‑1 (MQ=255)
                                                          cGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGTGAGCGTCACATccaccacca                               <  2:3586323/93‑1 (MQ=255)
                                                          cGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGGGAGCGTCACATccaccacca                               <  1:5298544/93‑1 (MQ=255)
                                                           gACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGGGAGCGTCACATCCACCACCAg                              <  2:6299423/93‑1 (MQ=255)
                                                           gACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGGGAGCGTCACATCCACCACCAg                              <  2:4595157/93‑1 (MQ=255)
                                                           gACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGGGAGCGTCACATCCACCACCAg                              <  2:3787970/93‑1 (MQ=255)
                                                           gACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGGGAGCGTCACATCCACCACCAg                              <  1:1358070/93‑1 (MQ=255)
                                                           gACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGGGAGCGTCACATCCACCACCAg                              >  1:3267457/1‑93 (MQ=255)
                                                           gACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGGGAGCGTCAAATCCCCCACCAg                              >  1:5154586/1‑93 (MQ=255)
                                                            aCAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGGGAGCGTCACATCCACCACCAgg                             <  1:5835481/93‑1 (MQ=255)
                                                            aCAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGCCACGCGCATCTTCCATAGGAACCTGCACGCCAGCGAGCGTCACATCCACCACCAgg                             >  2:5811177/1‑93 (MQ=255)
                                                             cAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGGGAGCGTCACATCCACCACCAGGt                            <  1:5975498/93‑1 (MQ=255)
                                                               gCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGGGAGCGTCACATCCACCACCAGGTgc                          <  1:2511207/93‑1 (MQ=255)
                                                               gCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGGGAGCGTCACATCCACCACCAGGTgc                          <  1:3556692/93‑1 (MQ=255)
                                                                 aCGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGGGAGCGTCGCATCCGCCACCAGGTgcgg                        >  2:1240931/1‑93 (MQ=255)
                                                                    cggcgCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGGGAGCGTCACATCCACCACCAGGTGCGGCGt                     <  1:3351698/93‑1 (MQ=255)
                                                                      gcgcAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGGGAGCGTCACATCCACCACCAGGTGCGGCGTGa                   >  1:1051527/1‑93 (MQ=255)
                                                                      gcgcAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGGGAGCGTCACATCCACCACCAGGTGCGGCGTGa                   >  2:1609418/1‑93 (MQ=255)
                                                                      gcgcAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGGGAGCGTCACATCCACCACCAGGTGCGGCGTGa                   >  1:5627281/1‑93 (MQ=255)
                                                                         cAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGGGAGCGTCACATCCACCACCAGGTGCGGCGTGAGCt                >  1:2966532/1‑93 (MQ=255)
                                                                          aaTGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGGGAGCGTCACATCCACCACCAGGTGCGGCGTGAGCTg               >  2:1880097/1‑93 (MQ=255)
                                                                          aaTGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGGGAGCGTCACATCCACCACCAGGTGCGGCGTGAGCTg               <  2:1515415/93‑1 (MQ=255)
                                                                          aaTGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGGGAGCGTCACATCCACCACCAGGTGCGGCGTGAGCTg               <  2:5908413/93‑1 (MQ=255)
                                                                          aaTGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGGGAGCGTCACATCCACCACCAGGTGCGGCGTGAGCTg               >  1:5711378/1‑93 (MQ=255)
                                                                          aaTGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCCGCACGCCAGGGAGCGTCACATCCACCACCAGGTGCGGCGTGAGCTg               >  1:2167350/1‑93 (MQ=255)
                                                                           aTGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGGGAGCGTCACATCCACCACCAGGTGCGGCGTGAGCTgg              <  1:3719516/93‑1 (MQ=255)
                                                                             gTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGGGAGCGTCACATCCACCACCAGGTGCGGCGTGAGCTGGtt            >  2:749668/1‑93 (MQ=255)
                                                                              ttGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGGGAGCGTCACATCCACCACCAGGTGCGGCGTGAGCTGGTTa           <  1:1293094/93‑1 (MQ=255)
                                                                               tGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGGGAGCGTCACATCCACCACCAGGTGCGGCGTGAGCTGGTTAt          <  2:203661/93‑1 (MQ=255)
                                                                               tGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGGGAGCGTCACATCCACCACCAGGTGCGGCGTGAGCTGGTTAt          <  2:4648059/93‑1 (MQ=255)
                                                                                gAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGGGAGCGTCACATCCACCACCAGGTGCGGCGTGAGCTGGTTATc         <  1:4989438/93‑1 (MQ=255)
                                                                                gAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGGGAGCGTCACATCCACCACCAGGTGCGGCGTGAGCTGGTTATc         >  1:2663071/1‑93 (MQ=255)
                                                                                 aGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGGGAGCGTCACATCCACCACCAGGTGCGGCGTGAGCTGGTTATcc        <  1:1525747/93‑1 (MQ=255)
                                                                                    aCGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGGGAGCGTCACATCCACCACCAGGTGCGGCGTGAGCTGGTTATCCAGc     >  1:1935565/1‑93 (MQ=255)
                                                                                     cGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGGGAGCGTCACTTCCACCACCAGGTGCGGCGTGAGCTGGTTATCCAGCa    <  1:4621019/93‑1 (MQ=255)
                                                                                      gATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGGGAGCGTCACATCCCCCACCAGGTGCGGCGTGAGCTGGTTATCCAGCaa   <  1:2601927/93‑1 (MQ=255)
                                                                                       aTTCGCCCGTCACGCTCTTATTCCATAGGAACCTGCACGCCAGGGAGCGTCACATCCACCACCAGGTGCGGCGTGAGCTGGTTATCCAGCAAc  <  2:4380353/93‑1 (MQ=255)
                                                                                       aTTCGCCCGTCACGCTCATATTCCATAGGAACCTGCACGCCAGGGAGCGTCACATCCCCCACCAGGTGCGGCGTGAGCTGGTTATCCAGCAAc  <  1:5324169/93‑1 (MQ=255)
                                                                                       aTTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGGGAGCGTCACATCCACCACCAGGTGCGGCGTGAGCTGGTTATCCAGCAAc  >  2:858200/1‑93 (MQ=255)
                                                                                          |                                                                                         
GGAATGCCACCAAAGCGCGCGTTAAAGCGCACCTCATCATTCGCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTTGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGGGAGCGTCACATCCACCACCAGGTGCGGCGTGAGCTGGTTATCCAGCAAC  >  NC_000913/3374173‑3374352

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGCGGAATGCCACCAAAGCGCGCGTTAAAGCGCACCTCATCATTCGCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTTGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGGGAGCGTCACATCCACCACCAGGTGCGGCGTGAGCTGGTTATCCAGCAACCACT  >  NC_000913/3374170‑3374356
                                                                                             |                                                                                             
CGCGGAATGCCACCAAAGCGCGCGTTAAAGCGCACCTCATCATTCGCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTC                                                                                               >  SRR4302533.3235474/1‑94 (MQ=60)
 GCGGAATGCCACCAAAGCGCGCGTTAAAGCGCACCTCATCATTCGCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCG                                                                                              >  SRR4302533.20649/1‑94 (MQ=60)
  CGGAATGCCACCAAAGCGCGCGTTAAAGCGCACCTCATCATTCGCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGC                                                                                             >  SRR4302533.6022575/1‑94 (MQ=60)
   GGAATGCCACCAAAGCGCGCGTTAAAGCGCACCTCATCATTCGCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCC                                                                                            >  SRR4302533.1691759/1‑94 (MQ=60)
   GGAATGCCACCAAAGCGCGCGTTAAAGCGCACCTCATCATTCGCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCC                                                                                            >  SRR4302533.5850819/1‑94 (MQ=60)
   GGAATGCCACCAAAGCGCGCGTTAAAGCGCACCTCATCATTCGCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCC                                                                                            >  SRR4302533.5570960/1‑94 (MQ=60)
    GAATGCCACCAAAGCGCGCGTTAAAGCGCACCTCATCATTCGCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCC                                                                                           >  SRR4302533.4391222/1‑94 (MQ=60)
    GAATGCCACCAAAGCGCGCGTTAAAGCGCACCTCATCATTCGCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCC                                                                                           <  SRR4302533.4080526/94‑1 (MQ=60)
    GAATGCCACCAAAGCGCGCGTTAAAGCGCACCTCATCATTCGCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCC                                                                                           <  SRR4302533.4845031/94‑1 (MQ=60)
    GAATGCCACCAAAGCGCGCGTTAAAGCGCACCTCATCATTCGCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCC                                                                                           >  SRR4302533.5055587/1‑94 (MQ=60)
    GAATGCCACCAAAGCGCGCGTTAAAGCGCACCTCATCATTCGCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCC                                                                                           >  SRR4302533.2614330/1‑94 (MQ=60)
    GAATGCCACCAAAGCGCGCGTTAAAGCGCACCTCATCATTCGCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCC                                                                                           <  SRR4302533.2976905/94‑1 (MQ=60)
     AATGCCACCAAAGCGCGCGTTAAAGCGCACCTCATCATTCGCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCG                                                                                          >  SRR4302533.3078986/1‑94 (MQ=60)
     AATGCCACCAAAGCGCGCGTTAAAGCGCACCTCATCATTCGCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCG                                                                                          >  SRR4302533.2782805/1‑94 (MQ=60)
      ATGCCACCAAAGCGCGCGTTAAAGCGCACCTCATCATTCGCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGT                                                                                         >  SRR4302533.4750070/1‑94 (MQ=60)
       TGCCACCAAAGCGCGCGTTAAAGCGCACCTCATCATTCGCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTC                                                                                        >  SRR4302533.1524255/1‑94 (MQ=60)
        GCCACCAAAGCGCGCGTTAAAGCGCACCTCATCATTCGCCATTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCA                                                                                       <  SRR4302533.151197/94‑1 (MQ=60)
        GCCACCAAAGCGCGCGTTAAAGCGCACCTCATCATTCGCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCA                                                                                       <  SRR4302533.2585325/94‑1 (MQ=60)
        GCCACCAAAGCGCGCGTTAAAGCGCACCTCATCATTCGCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCA                                                                                       >  SRR4302533.3384561/1‑94 (MQ=60)
        GCCACCAAAGCGCGCGTTAAAGCGCACCTCATCATTCGCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCA                                                                                       <  SRR4302533.6319695/94‑1 (MQ=60)
        GCCACCAAAGCGCGCGTTAAAGCGCACCTCATCATTCGCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCA                                                                                       <  SRR4302533.4344577/94‑1 (MQ=60)
        GCCACCAAAGCGCGCGTTAAAGCGCACCTCATCATTCGCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCA                                                                                       <  SRR4302533.1057676/94‑1 (MQ=60)
         CCACCAAAGCGCGCGTTAAAGCGCACCTCATCATTCGCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCAC                                                                                      <  SRR4302533.3801290/94‑1 (MQ=60)
         CCACCAAAGCGCGCGTTAAAGCGCACCTCATCATTCGCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCAC                                                                                      >  SRR4302533.1891566/1‑94 (MQ=60)
          CACCAAAGCGCGCGTTAAAGCGCACCTCATCATTCGCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACG                                                                                     <  SRR4302533.689239/94‑1 (MQ=60)
          CACCAAAGCGCGCGTTAAAGCGCACCTCATCATTCGCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACG                                                                                     >  SRR4302533.6092848/1‑94 (MQ=60)
          CACCAAAGCGCGCGTTAAAGCGCACCTCATCATTCGCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACG                                                                                     >  SRR4302533.1329714/1‑94 (MQ=60)
          CACCAAAGCGCGCGTTAAAGCGCACCTCATCATTCGCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACG                                                                                     >  SRR4302533.2286504/1‑94 (MQ=60)
            CCAAAGCGCGCGTTAAAGCGCACCTCATCATTCGCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCG                                                                                   <  SRR4302533.2595027/94‑1 (MQ=60)
             CAAAGCGCGCGTTAAAGCGCACCTCATCATTCGCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGC                                                                                  >  SRR4302533.1651483/1‑94 (MQ=60)
             CAAAGCGCGCGTTAAAGCGCACCTCATCATTCGCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGC                                                                                  <  SRR4302533.3571588/94‑1 (MQ=60)
                AGCGCGCGTTAAAGCGCACCTCATCATTCGCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATA                                                                               >  SRR4302533.1614568/1‑94 (MQ=60)
                 GCGCGCGTTAAAGCGCACCTCATCATTCGCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATAT                                                                              <  SRR4302533.3186397/94‑1 (MQ=60)
                 GCGCGCGTTAAAGCGCACCTCATCATTCGCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATAT                                                                              <  SRR4302533.47343/94‑1 (MQ=60)
                 GCGCGCGTTAAAGCGCACCTCATCATTCGCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATAT                                                                              <  SRR4302533.3240216/94‑1 (MQ=60)
                   GCGCGTTAAAGCGCACCTCATCATTCGCCAGTTCCAGATTGCCGACAGCCCGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGAGCATATTC                                                                            >  SRR4302533.1887905/1‑94 (MQ=60)
                   GCGCGTTAAAGCGCACCTCATCATTCGCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTC                                                                            <  SRR4302533.2906957/94‑1 (MQ=60)
                   GCGCGTTAAAGCGCACCTCATCATTCGCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTC                                                                            <  SRR4302533.2387738/94‑1 (MQ=60)
                   GCGCGTTAAAGCGCACCTCATCATTCGCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTC                                                                            <  SRR4302533.3716111/94‑1 (MQ=60)
                    CGCGTTAAAGCGCACCTCATCATTCGCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCC                                                                           >  SRR4302533.389021/1‑94 (MQ=60)
                    CGCGTTAAAGCGCACCTCATCATTCGCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCC                                                                           >  SRR4302533.3039664/1‑94 (MQ=60)
                     GCGTTAAAGCGCACCTCATCATTCGCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCA                                                                          >  SRR4302533.3372007/1‑94 (MQ=60)
                     GCGTTAAAGCGCACCTCATCATTCGCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCA                                                                          >  SRR4302533.1837309/1‑94 (MQ=60)
                      CGTTAAAGCGCACCTCATCATTCGCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCAT                                                                         <  SRR4302533.5427628/94‑1 (MQ=60)
                      CGTTAAAGCGCACCTCATCATTCGCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCAT                                                                         >  SRR4302533.4172606/1‑94 (MQ=60)
                      CGTTAAAGCGCACCTCATCATTCGCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCAT                                                                         >  SRR4302533.59761/1‑94 (MQ=60)
                       GTTAAAGCGCACCTCATCATTCGCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATA                                                                        >  SRR4302533.4441187/1‑94 (MQ=60)
                       GTTAAAGAGCACCTCATCATTCGCCAGTTCCAGATTG‑CGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAG                                                                       >  SRR4302533.5799363/1‑94 (MQ=60)
                        TTAAAGCTCACCTCATCATTCGCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAG                                                                       <  SRR4302533.3193506/94‑1 (MQ=60)
                        TTAAAGCGCACCTCATCATTCGCCAGTTCCAGATTGCCGACAGCACTCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTACATAG                                                                       >  SRR4302533.3949087/1‑94 (MQ=60)
                        TTAAAGCGCACCTCATCATTCGCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAG                                                                       <  SRR4302533.4814432/94‑1 (MQ=60)
                        TTAAAGCGCACCTCATCATTCGCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAG                                                                       <  SRR4302533.3394841/94‑1 (MQ=60)
                          AAAGCGCACCTCATCATTCGCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGA                                                                     <  SRR4302533.4454332/94‑1 (MQ=60)
                           AAGCGCACCTCATCATTCGCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAA                                                                    <  SRR4302533.2972622/94‑1 (MQ=60)
                            AGCGCACCTCATCATTCGCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAAC                                                                   >  SRR4302533.3045167/1‑94 (MQ=60)
                              CGCACCTCATCATTCGCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCT                                                                 >  SRR4302533.3320897/1‑94 (MQ=60)
                              CGCACCTCATCATTCGCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCT                                                                 <  SRR4302533.3976475/94‑1 (MQ=60)
                              CGCACCTCATCATTCGCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCT                                                                 >  SRR4302533.1052230/1‑94 (MQ=60)
                              CGCACCTCATCATTCGCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCT                                                                 <  SRR4302533.1612714/94‑1 (MQ=60)
                              CGCACCTCATCATTCGCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCT                                                                 <  SRR4302533.1698601/94‑1 (MQ=60)
                              CGCACCTCATCATTCGCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCT                                                                 >  SRR4302533.1850698/1‑94 (MQ=60)
                               GCACCTCATCATTCGCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTG                                                                >  SRR4302533.690952/1‑94 (MQ=60)
                                CACCTCATCATTCGCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGC                                                               >  SRR4302533.5676101/1‑94 (MQ=60)
                                CACCTCATCATTCGCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGC                                                               >  SRR4302533.1662143/1‑94 (MQ=60)
                                 ACCTCATCATTCGCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGNGTACGATTCGCCCGNCACNNNCATATTCCATAGGNNNNTNCA                                                              >  SRR4302533.5943735/1‑94 (MQ=60)
                                   CTCATCATTCGCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACG                                                            >  SRR4302533.1614293/1‑94 (MQ=60)
                                   CTCATCATTCGCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACC                                                            >  SRR4302533.1195593/1‑94 (MQ=60)
                                    TCATCATTCGCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGC                                                           >  SRR4302533.2039856/1‑94 (MQ=60)
                                      ATCATTCGCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCA                                                         <  SRR4302533.2619826/94‑1 (MQ=60)
                                       TCATTCGCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAG                                                        <  SRR4302533.2572649/94‑1 (MQ=60)
                                       TCATTCGCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAG                                                        <  SRR4302533.2810836/94‑1 (MQ=60)
                                        CATTCGCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGG                                                       >  SRR4302533.1462202/1‑94 (MQ=60)
                                        CATTCGCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGG                                                       <  SRR4302533.1679608/94‑1 (MQ=60)
                                         ATTCGCCAGTTCCAGATTGCCGACAGCACGCGGTGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGGG                                                      >  SRR4302533.285206/1‑94 (MQ=60)
                                         ATTCGCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGGG                                                      <  SRR4302533.1610222/94‑1 (MQ=60)
                                          TTCGCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGGGA                                                     <  SRR4302533.668526/94‑1 (MQ=60)
                                           TCGCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGGGAG                                                    <  SRR4302533.2121431/94‑1 (MQ=60)
                                           TCGCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGGGAG                                                    <  SRR4302533.1895531/94‑1 (MQ=60)
                                            CGCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGGGAGC                                                   >  SRR4302533.479604/1‑94 (MQ=60)
                                             GCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGGGAGCG                                                  >  SRR4302533.4018636/1‑94 (MQ=60)
                                                AGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGGGAGCGTCA                                               <  SRR4302533.5479400/94‑1 (MQ=60)
                                                  TTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGGGAGCGTCACA                                             >  SRR4302533.1061727/1‑94 (MQ=60)
                                                   TCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGGGAGCGTCACAT                                            >  SRR4302533.1775727/1‑94 (MQ=60)
                                                    CCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGGGAGCGTCACATC                                           <  SRR4302533.6056714/94‑1 (MQ=60)
                                                     CAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGGGAGCGTCACATCC                                          <  SRR4302533.2550689/94‑1 (MQ=60)
                                                     CAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGGGAGCGTCACATCC                                          <  SRR4302533.1646334/94‑1 (MQ=60)
                                                       GATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCCCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGGGAGCGTCACATCCAC                                        <  SRR4302533.2134828/94‑1 (MQ=60)
                                                        ATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGGGAGCGTCACATCCACC                                       >  SRR4302533.4945174/1‑94 (MQ=60)
                                                        ATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGGGAGCGTCACATCCACC                                       <  SRR4302533.63253/94‑1 (MQ=60)
                                                        ATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGGGAGCGTCACATCCACC                                       >  SRR4302533.5484520/1‑94 (MQ=60)
                                                        ATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGGGAGCGTCACATCCACC                                       >  SRR4302533.5855284/1‑94 (MQ=60)
                                                           GCCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGGGAGCGTCACATCCACCACC                                    <  SRR4302533.2849426/94‑1 (MQ=60)
                                                            CCGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGGGAGCGTCACATCCAACACCA                                   >  SRR4302533.5390952/1‑94 (MQ=60)
                                                             CGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGGGAGCGTCACATCCACCACCAG                                  >  SRR4302533.1444747/1‑94 (MQ=60)
                                                             CGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGGGAGCGTCACATCCACCACCAG                                  <  SRR4302533.5330285/94‑1 (MQ=60)
                                                             CGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGGGAGCGTCACATCCACCACCAG                                  <  SRR4302533.3917845/94‑1 (MQ=60)
                                                             CGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGGGAGCGTCACATCCACCACCAG                                  >  SRR4302533.3287621/1‑94 (MQ=60)
                                                             CGACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGGGAGCGTCAAATCCCCCACCAG                                  >  SRR4302533.5185715/1‑94 (MQ=60)
                                                              GACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGGGAGCGTCACATCCACCACCAGG                                 <  SRR4302533.1366233/94‑1 (MQ=60)
                                                              GACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGGGAGCGTCACATCCACCACCAGG                                 <  SRR4302533.4963590/94‑1 (MQ=60)
                                                              GACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGGGAGCGTCACATCCACCACCAGG                                 >  SRR4302533.2547429/1‑94 (MQ=60)
                                                              GACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGGGAGCGTCACATCCACCACCAGG                                 <  SRR4302533.1945253/94‑1 (MQ=60)
                                                               ACAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGGGAGCGTCACATCCACCACCAGGT                                <  SRR4302533.5870743/94‑1 (MQ=60)
                                                                CAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGGGAGCGTCACATCCACCACCAGGTG                               <  SRR4302533.6011670/94‑1 (MQ=60)
                                                                CAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGGGAGCGTCACATCCACCACCAGGTG                               >  SRR4302533.2249217/1‑94 (MQ=60)
                                                                CAGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGGGAGCGTCACATCCACCACCAGGTG                               <  SRR4302533.1756/94‑1 (MQ=60)
                                                                 AGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGGGAGCGTCACATCCACCACCAGGTGC                              >  SRR4302533.15598/1‑94 (MQ=60)
                                                                 AGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGGGAGCGTCACATCCACCACCAGGTGC                              >  SRR4302533.2610421/1‑94 (MQ=60)
                                                                 AGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGGGAGCGTCACATCCACCACCAGGTGC                              >  SRR4302533.3989459/1‑94 (MQ=60)
                                                                 AGCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGGGAGCGTCACATCCACCACCAGGTCC                              <  SRR4302533.344025/94‑1 (MQ=60)
                                                                  GCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGGGAGCGTCACATCCACCACCAGGTGCG                             <  SRR4302533.2526681/94‑1 (MQ=60)
                                                                  GCACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGGGAGCGTCACATCCACCACCAGGTGCG                             <  SRR4302533.3578612/94‑1 (MQ=60)
                                                                   CACGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGGGAGCGTCACATCCACCACCAGGTGCGG                            <  SRR4302533.5913342/94‑1 (MQ=60)
                                                                     CGCGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGGGAGCGTCACATCCACCACCAGGTGCGGCG                          >  SRR4302533.3788979/1‑94 (MQ=60)
                                                                       CGGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGGGAGCGTCACATCCACCACCAGGTGCGGCGTG                        <  SRR4302533.3372402/94‑1 (MQ=60)
                                                                        GGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGGGAGCGTCACATCCACCACCAGGTGCGGCGTGA                       >  SRR4302533.1057718/1‑94 (MQ=60)
                                                                        GGCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGGGAGCGTCACATCCACCACCAGGTGCGGCGTGA                       >  SRR4302533.5661304/1‑94 (MQ=60)
                                                                         GCGCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGGGAGCGTCACATCCACCACCAGGTGCGGCGTGAG                      >  SRR4302533.5315651/1‑94 (MQ=60)
                                                                           GCAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGGGAGCGTCACATCCACCACCAGGTGCGGCGTGAGCT                    >  SRR4302533.2984663/1‑94 (MQ=60)
                                                                            CAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGGGAGCGTCACATCCACCACCAGGTGCGGCGTGAGCTG                   >  SRR4302533.5745877/1‑94 (MQ=60)
                                                                            CAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGGGAGCGTCACATCCACCACCAGGTGCGGCGTGAGCTG                   <  SRR4302533.5354647/94‑1 (MQ=60)
                                                                            CAATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCCGCACGCCAGGGAGCGTCACATCCACCACCAGGTGCGGCGTGAGCTG                   >  SRR4302533.2180680/1‑94 (MQ=60)
                                                                              ATGTTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGGGAGCGTCACATCCACCACCAGGTGCGGCGTGAGCTGGT                 <  SRR4302533.3742348/94‑1 (MQ=60)
                                                                                 TTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGGGAGCGTCACATCCACCACCAGGTGCGGCGTGAGCTGGTTAT              >  SRR4302533.2217878/1‑94 (MQ=60)
                                                                                 TTGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGGGAGCGTCACATCCACCACCAGGTGCGGCGTGAGCTGGTTAT              <  SRR4302533.1300884/94‑1 (MQ=60)
                                                                                  TGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGGGAGCGTCACATCCACCACCAGGTGCGGCGTGAGCTGGTTATC             >  SRR4302533.5572663/1‑94 (MQ=60)
                                                                                  TGAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGGGAGCGTCACATCCACCACCAGGTGCGGCGTGAGCTGGTTATC             >  SRR4302533.2679421/1‑94 (MQ=60)
                                                                                   GAGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGGGAGCGTCACATCCACCACCAGGTGCGGCGTGAGCTGGTTATCC            <  SRR4302533.5019688/94‑1 (MQ=60)
                                                                                    AGTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGGGAGCGTCACATCCACCACCAGGTGCGGCGTGAGCTGGTTATCCA           <  SRR4302533.1534931/94‑1 (MQ=60)
                                                                                     GTACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGGGAGCGTCACATCCACCACCAGGTGCGGCGTGAGCTGGTTATCCAG          >  SRR4302533.2058739/1‑94 (MQ=60)
                                                                                      TACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGGGAGCGTCACATCCACCACCAGGTGCGGCGTGAGCTGGTTATCCAGC         >  SRR4302533.1947372/1‑94 (MQ=60)
                                                                                       ACGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGGGAGCGTCACATCCACCACCAGGTGNGGCGTGAGCTGGTTATCCAGCA        >  SRR4302533.5634867/1‑94 (MQ=60)
                                                                                        CGATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGGGAGCGTCACTTCCACCACCAGGTGCGGCGTGAGCTGGTTATCCAGCAA       <  SRR4302533.4649026/94‑1 (MQ=60)
                                                                                         GATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGGGAGCGTCACATCCCCCACCAGGTGCGGCGTGAGCTGGTTATCCAGCAAC      <  SRR4302533.2617916/94‑1 (MQ=60)
                                                                                         GATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGGGAGCGTCACATCCACCACCAGGTGCGGCGTGAGCTGGTTATCCAGCAAC      <  SRR4302533.5836247/94‑1 (MQ=60)
                                                                                          ATTCGCCCGTCACGCTCATATTCCATAGGAACCTGCACGCCAGGGAGCGTCACATCCCCCACCAGGTGCGGCGTGAGCTGGTTATCCAGCAACC     <  SRR4302533.5356077/94‑1 (MQ=60)
                                                                                          ATTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGGGAGCGTCACATCCACCACCAGGTGCGGCGTGAGCTGGTTATCCAGCAACC     <  SRR4302533.5265192/94‑1 (MQ=60)
                                                                                           TTCGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGGGAGCGTCACATCCACCACCAGGTGCGGCGTGAGCTGGTTATCCAGCAACCA    >  SRR4302533.5169960/1‑94 (MQ=60)
                                                                                             CGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGGGAGCGTCACATCCACCACCAGGTGCGGCGTGAGCTGGTTATCCAGCAACCACT  >  SRR4302533.6010262/1‑94 (MQ=60)
                                                                                             CGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGGGAGCGTCACATCCACCACCAGGTGCGGCGTGAGCTGGTTATCCAGCAACCACT  <  SRR4302533.5681921/94‑1 (MQ=60)
                                                                                             |                                                                                             
CGCGGAATGCCACCAAAGCGCGCGTTAAAGCGCACCTCATCATTCGCCAGTTCCAGATTGCCGACAGCACGCGGCGCAATGTTGAGTACGATTTGCCCGTCACGCGCATATTCCATAGGAACCTGCACGCCAGGGAGCGTCACATCCACCACCAGGTGCGGCGTGAGCTGGTTATCCAGCAACCACT  >  NC_000913/3374170‑3374356

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑