Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,393,106	Δ1 bp	intergenic (‑3443/+857)	tldD ← / ← cafA	TldD/CafA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,393,103	0	T	.	99.6%	1059.1 / ‑3.0	230	intergenic (‑3440/+860)	tldD/cafA	TldD/CafA
Reads supporting (aligned to +/- strand): ref base T (0/0); major base . (126/103); minor base A (1/0); total (127/103)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.34e-01

Only 100 of 256 total aligned reads displayed.

CAGCCCGGATTTTCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTGTTTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATA  >  NC_000913/3393011‑3393191

cAGCCCGGATTTTCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTGt                                                                                          >  1:409777/1‑93 (MQ=255)

cAGCCCGGATTTTCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTGt                                                                                          <  2:5251092/93‑1 (MQ=255)

 aGCCCGGATTTTCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTGtt                                                                                         <  2:2948166/93‑1 (MQ=255)

  gCCCGGATTTTCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTGttt                                                                                        >  1:754469/1‑93 (MQ=255)

   cccGGATTTTCACGCGTAATATGCGCGGTCAGATTATCCCCCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTGtttc                                                                                       <  1:3775412/93‑2 (MQ=255)

     cGGATTTTCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGTTATCGCCCTGttttac                                                                                     >  2:39288/1‑91 (MQ=255)

      ggATTTTCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTGtttcagc                                                                                    >  2:2063574/1‑89 (MQ=255)

      ggATTTTCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTGtttcagc                                                                                    >  2:1717606/1‑89 (MQ=255)

       gATTTTCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGcc                                                                                  <  2:4807399/93‑1 (MQ=255)

        aTTTTCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCa                                                                                 >  1:5564664/1‑93 (MQ=255)

         ttttCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAg                                                                                <  1:1611179/93‑1 (MQ=255)

           ttCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGAc                                                                              <  2:4664400/93‑1 (MQ=255)

           ttCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGAc                                                                              >  1:295590/1‑93 (MQ=255)

           ttCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGAc                                                                              <  1:2106948/93‑1 (MQ=255)

             cACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGt                                                                            <  1:1354259/93‑1 (MQ=255)

                gcgTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCAc                                                                         >  1:4942808/1‑93 (MQ=255)

                 cgTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCAcc                                                                        >  1:4643257/1‑93 (MQ=255)

                 cgTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCAcc                                                                        >  2:6197304/1‑93 (MQ=255)

                 cgTAATACGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCAcc                                                                        <  1:6212453/93‑1 (MQ=255)

                   tAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCAccgc                                                                      <  1:680500/93‑1 (MQ=255)

                   tAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCAccgc                                                                      <  1:1909518/93‑1 (MQ=255)

                   tAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCAccgc                                                                      <  2:717873/93‑1 (MQ=255)

                    aaTATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCAccgcc                                                                     >  2:5876089/1‑93 (MQ=255)

                    aaTATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCAccgcc                                                                     >  1:4664155/1‑93 (MQ=255)

                       atGCGCGGTCATATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGt                                                                  <  1:3601036/93‑1 (MQ=255)

                          cgcgGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTAc                                                               >  2:1207335/1‑93 (MQ=255)

                           gcgGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACa                                                              <  2:2141199/93‑1 (MQ=255)

                           gcgGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACa                                                              >  1:5494018/1‑93 (MQ=255)

                            cgGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACAt                                                             <  2:1040913/93‑1 (MQ=255)

                             ggTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATc                                                            >  1:4538657/1‑93 (MQ=255)

                             ggTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATc                                                            <  1:4788575/93‑1 (MQ=255)

                              gTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCg                                                           >  1:1091115/1‑93 (MQ=255)

                               tCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGc                                                          >  1:5068399/1‑93 (MQ=255)

                               tCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGc                                                          <  2:2335042/93‑1 (MQ=255)

                                cAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGcc                                                         <  2:4259778/93‑1 (MQ=255)

                                 aGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCt                                                        >  2:1420347/1‑93 (MQ=255)

                                  gATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCtt                                                       >  2:6127711/1‑93 (MQ=255)

                                  gATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCtt                                                       <  1:2382536/93‑1 (MQ=255)

                                  gATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCAAGACGTCACCGCCGGTTACATCGCCtt                                                       >  1:4223910/1‑93 (MQ=255)

                                    ttATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTg                                                     >  2:3852036/1‑93 (MQ=255)

                                      aTCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTc                                                   <  2:2509454/93‑1 (MQ=255)

                                      aTCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTc                                                   <  1:2881258/93‑1 (MQ=255)

                                       tCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCg                                                  >  2:2425871/1‑93 (MQ=255)

                                       tCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCg                                                  <  1:5326207/93‑1 (MQ=255)

                                       tCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCACCTTTGTCg                                                  <  2:4120941/93‑1 (MQ=255)

                                          aCCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATc                                               >  1:55015/1‑93 (MQ=255)

                                           ccGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCg                                              <  2:1565688/93‑1 (MQ=255)

                                             gACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTc                                            <  1:1273224/93‑1 (MQ=255)

                                             gACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTc                                            <  2:1650320/93‑1 (MQ=255)

                                             gACAGCGTATGCGTTTGCTCCTCGCCCACCCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTc                                            <  2:3564043/93‑1 (MQ=255)

                                              agaGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTTGATCGtga                                           >  2:6030686/3‑91 (MQ=255)

                                              aCAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCa                                           >  1:573464/1‑93 (MQ=255)

                                               cAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCAt                                          >  1:1235224/1‑93 (MQ=255)

                                                aGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATc                                         >  1:2801346/1‑93 (MQ=255)

                                                aGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATc                                         <  1:5709453/93‑1 (MQ=255)

                                                 gCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATcc                                        >  2:2308790/1‑93 (MQ=255)

                                                 gCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATcc                                        <  2:5856419/93‑1 (MQ=255)

                                                  cGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGCTCGTCATCCa                                       >  1:2549308/1‑93 (MQ=255)

                                                  cGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCa                                       <  1:2063574/93‑1 (MQ=255)

                                                  cGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCa                                       <  1:865250/93‑1 (MQ=255)

                                                  cGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCa                                       <  2:2655174/93‑1 (MQ=255)

                                                    tATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGc                                     >  2:5274040/1‑93 (MQ=255)

                                                    tATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGc                                     >  2:1340500/1‑93 (MQ=255)

                                                     aTGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGcc                                    >  2:5028225/1‑93 (MQ=255)

                                                      tGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCt                                   <  1:3053830/93‑1 (MQ=255)

                                                      tGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCt                                   <  2:5191477/93‑1 (MQ=255)

                                                          tttGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCaa                               >  2:3195636/1‑93 (MQ=255)

                                                           ttGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAg                              <  1:5863729/93‑1 (MQ=255)

                                                            tGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAgg                             >  2:2485182/1‑93 (MQ=255)

                                                            tGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAgg                             >  1:5642989/1‑93 (MQ=255)

                                                            tGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAgg                             <  1:4407281/93‑1 (MQ=255)

                                                             tcTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAggg                            <  1:1998739/92‑1 (MQ=255)

                                                             gCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACCTCGCCTTTGTCGATCGTCATCCAGCCTTCGAggg                            >  1:3928553/1‑93 (MQ=255)

                                                             gCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAggg                            <  2:6302315/93‑1 (MQ=255)

                                                             gCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAggg                            >  2:4725353/1‑93 (MQ=255)

                                                              cTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGga                           >  1:62546/1‑93 (MQ=255)

                                                              cTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGga                           >  2:2804200/1‑93 (MQ=255)

                                                              cTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGga                           <  2:1494778/93‑1 (MQ=255)

                                                                 ctcGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCTGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGgagag                        >  1:1769280/1‑92 (MQ=255)

                                                                  tcGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAAc                       >  2:3419873/1‑93 (MQ=255)

                                                                  ccGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGGCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAAc                       <  2:5393223/92‑1 (MQ=255)

                                                                   cGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACt                      <  1:4612076/93‑1 (MQ=255)

                                                                    gCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACtg                     <  1:1137603/93‑1 (MQ=255)

                                                                    gCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACtg                     <  2:2995245/93‑1 (MQ=255)

                                                                     cccAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGCTACATCGCCTTTGTCGATCGTCATCCAGTCTTCAAGGGAGCACtgt                    >  2:3308987/1‑93 (MQ=255)

                                                                      ccAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAGCtgtg                   >  2:4137360/1‑93 (MQ=255)

                                                                      ccAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACtgtg                   <  2:5601549/93‑1 (MQ=255)

                                                                        aaCCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGcc                 <  2:1909222/93‑1 (MQ=255)

                                                                        aaCCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGcc                 >  2:4859967/1‑93 (MQ=255)

                                                                         aCCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCg                >  2:5449373/1‑93 (MQ=255)

                                                                          ccAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGt               >  1:1829464/1‑93 (MQ=255)

                                                                          ccAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGt               >  2:4313657/1‑93 (MQ=255)

                                                                            aGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGttt             <  1:1469141/93‑1 (MQ=255)

                                                                            aGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGttt             <  1:324610/93‑1 (MQ=255)

                                                                                ggCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAg         >  1:1314821/1‑93 (MQ=255)

                                                                                 gCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAgc        >  2:4451815/1‑93 (MQ=255)

                                                                                     cGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCaa    <  1:5798828/93‑1 (MQ=255)

                                                                                      gCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGACTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAat   >  2:3476182/1‑93 (MQ=255)

                                                                                       cccTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAata  >  1:2158026/1‑93 (MQ=255)

                                                                                       cccTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAata  >  1:2682694/1‑93 (MQ=255)

CAGCCCGGATTTTCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTGTTTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATA  >  NC_000913/3393011‑3393191

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CCGGATTTTCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTGTTTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATATTGTCCTG  >  NC_000913/3393015‑3393199

CCGGATTTTCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAG                                                                                            >  SRR4302533.5851645/1‑94 (MQ=60)

CCGGATTTTCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAG                                                                                            <  SRR4302533.1545530/94‑1 (MQ=60)

CCGGATTTTCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAG                                                                                            >  SRR4302533.4297045/1‑94 (MQ=60)

 CGGATTTTCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGC                                                                                           >  SRR4302533.5636939/1‑94 (MQ=60)

  GGATTTTCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCC                                                                                          >  SRR4302533.5182552/1‑94 (MQ=60)

   GATTTTCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCA                                                                                         <  SRR4302533.5528088/94‑1 (MQ=60)

   GATTTTCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCA                                                                                         >  SRR4302533.3821505/1‑94 (MQ=60)

   GATTTTCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCA                                                                                         >  SRR4302533.5598299/1‑94 (MQ=60)

     TTTTCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGA                                                                                       <  SRR4302533.1620949/94‑1 (MQ=60)

      TTTCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGAC                                                                                      <  SRR4302533.2814513/94‑1 (MQ=60)

      TTTCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGAC                                                                                      >  SRR4302533.297394/1‑94 (MQ=60)

      TTTCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGAC                                                                                      <  SRR4302533.1411347/94‑1 (MQ=60)

      TTTCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGAC                                                                                      <  SRR4302533.196799/94‑1 (MQ=60)

       TTCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACG                                                                                     <  SRR4302533.2119941/94‑1 (MQ=60)

       TTCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACG                                                                                     <  SRR4302533.5084157/94‑1 (MQ=60)

         CACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTC                                                                                   <  SRR4302533.1362399/94‑1 (MQ=60)

           CGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCAC                                                                                 <  SRR4302533.3694072/94‑1 (MQ=60)

           CGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCAC                                                                                 >  SRR4302533.4972730/1‑94 (MQ=60)

            GCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACC                                                                                >  SRR4302533.4671388/1‑94 (MQ=60)

            GCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACC                                                                                >  SRR4302533.2145997/1‑94 (MQ=60)

            GCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACC                                                                                >  SRR4302533.670256/1‑94 (MQ=60)

             CGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCG                                                                               <  SRR4302533.2209482/94‑1 (MQ=60)

             CGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCG                                                                               >  SRR4302533.3029943/1‑94 (MQ=60)

             CGTAATACGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCG                                                                               <  SRR4302533.6249704/94‑1 (MQ=60)

               TAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCC                                                                             <  SRR4302533.1921168/94‑1 (MQ=60)

               TAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCC                                                                             <  SRR4302533.684526/94‑1 (MQ=60)

               TAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCC                                                                             >  SRR4302533.4692416/1‑94 (MQ=60)

                 ATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGG                                                                           >  SRR4302533.4220250/1‑94 (MQ=60)

                   ATGCGCGGTCATATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTT                                                                         <  SRR4302533.3623194/94‑1 (MQ=60)

                   ATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTT                                                                         >  SRR4302533.1459054/1‑94 (MQ=60)

                    TGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTA                                                                        <  SRR4302533.5690327/94‑1 (MQ=60)

                      CGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACA                                                                      >  SRR4302533.5527162/1‑94 (MQ=60)

                        CGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATC                                                                    >  SRR4302533.4566136/1‑94 (MQ=60)

                         GGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCG                                                                   >  SRR4302533.5236188/1‑94 (MQ=60)

                         GGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCG                                                                   >  SRR4302533.5643796/1‑94 (MQ=60)

                         GGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCG                                                                   >  SRR4302533.1097560/1‑94 (MQ=60)

                         GGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCG                                                                   <  SRR4302533.4817581/94‑1 (MQ=60)

                         GGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCG                                                                   >  SRR4302533.6317144/1‑94 (MQ=60)

                          GTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGC                                                                  >  SRR4302533.5099005/1‑94 (MQ=60)

                           TCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCC                                                                 >  SRR4302533.5957733/1‑94 (MQ=60)

                             AGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCAAGACGTCACCGCCGGTTACATCGCCTT                                                               >  SRR4302533.4249446/1‑94 (MQ=60)

                              GATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTT                                                              <  SRR4302533.2397268/94‑1 (MQ=60)

                                TTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGT                                                            >  SRR4302533.4643187/1‑94 (MQ=60)

                                TTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGT                                                            <  SRR4302533.5588435/94‑1 (MQ=60)

                                 TATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCACCCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTC                                                           <  SRR4302533.820645/94‑1 (MQ=60)

                                  ATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCG                                                          >  SRR4302533.4664109/1‑94 (MQ=60)

                                  ATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCG                                                          <  SRR4302533.2898984/94‑1 (MQ=60)

                                   TCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGA                                                         <  SRR4302533.5358127/94‑1 (MQ=60)

                                     CACCGACCGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATC                                                       <  SRR4302533.1176365/94‑1 (MQ=60)

                                     CACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATC                                                       >  SRR4302533.55387/1‑94 (MQ=60)

                                     CACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATC                                                       <  SRR4302533.1812856/94‑1 (MQ=60)

                                     CACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATC                                                       <  SRR4302533.5531829/94‑1 (MQ=60)

                                      ACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCG                                                      >  SRR4302533.4440451/1‑94 (MQ=60)

                                         GACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCA                                                   <  SRR4302533.1280843/94‑1 (MQ=60)

                                         GACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCA                                                   <  SRR4302533.5760574/94‑1 (MQ=60)

                                         GACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCA                                                   >  SRR4302533.576845/1‑94 (MQ=60)

                                          ACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCAT                                                  >  SRR4302533.1242607/1‑94 (MQ=60)

                                           CAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATC                                                 >  SRR4302533.2818579/1‑94 (MQ=60)

                                            AGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCC                                                <  SRR4302533.5743938/94‑1 (MQ=60)

                                             GCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGCTCGTCATCCA                                               >  SRR4302533.2565000/1‑94 (MQ=60)

                                             GCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCA                                               <  SRR4302533.2031914/94‑1 (MQ=60)

                                             GCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCA                                               <  SRR4302533.2591264/94‑1 (MQ=60)

                                              CGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAG                                              <  SRR4302533.2076295/94‑1 (MQ=60)

                                              CGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAG                                              <  SRR4302533.293660/94‑1 (MQ=60)

                                              CGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAG                                              <  SRR4302533.870316/94‑1 (MQ=60)

                                              CGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAG                                              >  SRR4302533.4742178/1‑94 (MQ=60)

                                              CGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAG                                              >  SRR4302533.4742179/1‑94 (MQ=60)

                                               GTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGC                                             >  SRR4302533.4593891/1‑94 (MQ=60)

                                                  TGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTT                                          <  SRR4302533.3072539/94‑1 (MQ=60)

                                                   GCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTC                                         >  SRR4302533.1246231/1‑94 (MQ=60)

                                                    CGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCA                                        >  SRR4302533.1648233/1‑94 (MQ=60)

                                                    CGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCA                                        <  SRR4302533.4498750/94‑1 (MQ=60)

                                                     GTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAA                                       >  SRR4302533.3672892/1‑94 (MQ=60)

                                                     GTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAA                                       >  SRR4302533.496593/1‑94 (MQ=60)

                                                     GTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAA                                       <  SRR4302533.764804/94‑1 (MQ=60)

                                                       TTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGG                                     <  SRR4302533.5899180/94‑1 (MQ=60)

                                                       TTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGG                                     >  SRR4302533.5677098/1‑94 (MQ=60)

                                                       TTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGG                                     <  SRR4302533.3601964/94‑1 (MQ=60)

                                                        TGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACCTCGCCTTTGTCGATCGTCATCCAGCCTTCGAGGG                                    >  SRR4302533.3952540/1‑94 (MQ=60)

                                                        TGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGG                                    <  SRR4302533.4433895/94‑1 (MQ=60)

                                                        TGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGG                                    <  SRR4302533.1280941/94‑1 (MQ=60)

                                                        TGCTTCTCGCCCAACCAGCTGGCACCGCCCT‑ATTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGG                                    >  SRR4302533.134556/1‑94 (MQ=60)

                                                         TCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGA                                   <  SRR4302533.2011038/94‑1 (MQ=60)

                                                         GCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGA                                   >  SRR4302533.62957/1‑94 (MQ=60)

                                                           TTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGA                                 >  SRR4302533.1878699/1‑94 (MQ=60)

                                                            TCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCTGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAG                                >  SRR4302533.1780052/1‑94 (MQ=60)

                                                             CTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAAC                               >  SRR4302533.576829/1‑94 (MQ=60)

                                                              TCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACT                              <  SRR4302533.1801694/94‑1 (MQ=60)

                                                               CGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTG                             <  SRR4302533.4640019/94‑1 (MQ=60)

                                                               CGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTG                             >  SRR4302533.4691440/1‑94 (MQ=60)

                                                                GCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGT                            <  SRR4302533.1144370/94‑1 (MQ=60)

                                                                 CCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTG                           >  SRR4302533.4242971/1‑94 (MQ=60)

                                                                 CCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTG                           <  SRR4302533.5412796/94‑1 (MQ=60)

                                                                  CCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGC                          >  SRR4302533.2609094/1‑94 (MQ=60)

                                                                   CAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCC                         >  SRR4302533.2102255/1‑94 (MQ=60)

                                                                     ACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGT                       >  SRR4302533.1840633/1‑94 (MQ=60)

                                                                       CAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTGGATCGTCATCCAGCCTTCAAGGGAGTACTGTGCCGTTT                     >  SRR4302533.755067/1‑94 (MQ=60)

                                                                       CAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTT                     >  SRR4302533.2897059/1‑94 (MQ=60)

                                                                       CAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTT                     >  SRR4302533.2248921/1‑94 (MQ=60)

                                                                        AGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTC                    <  SRR4302533.1477998/94‑1 (MQ=60)

                                                                        AGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTC                    <  SRR4302533.326591/94‑1 (MQ=60)

                                                                         GCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCC                   <  SRR4302533.1202846/94‑1 (MQ=60)

                                                                         GCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCC                   <  SRR4302533.578212/94‑1 (MQ=60)

                                                                          CTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCA                  <  SRR4302533.1137294/94‑1 (MQ=60)

                                                                           TGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAG                 >  SRR4302533.1322708/1‑94 (MQ=60)

                                                                           TGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAG                 <  SRR4302533.2003302/94‑1 (MQ=60)

                                                                           TGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAG                 >  SRR4302533.2500153/1‑94 (MQ=60)

                                                                           TGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAG                 >  SRR4302533.2947370/1‑94 (MQ=60)

                                                                            GGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGC                >  SRR4302533.3806944/1‑94 (MQ=60)

                                                                            GGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGC                <  SRR4302533.5803995/94‑1 (MQ=60)

                                                                                CCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAA            <  SRR4302533.3221359/94‑1 (MQ=60)

                                                                                 CGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAAT           <  SRR4302533.5833861/94‑1 (MQ=60)

                                                                                  GCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATA          >  SRR4302533.928455/1‑94 (MQ=60)

                                                                                  GCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATA          >  SRR4302533.2171311/1‑94 (MQ=60)

                                                                                  GCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATA          >  SRR4302533.6271639/1‑94 (MQ=60)

                                                                                  GCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATA          >  SRR4302533.2699131/1‑94 (MQ=60)

                                                                                   CCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATAT         >  SRR4302533.6161103/1‑94 (MQ=60)

                                                                                   CCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATAT         <  SRR4302533.3205195/94‑1 (MQ=60)

                                                                                    CCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATATT        >  SRR4302533.4238954/1‑94 (MQ=60)

                                                                                    CCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATATT        <  SRR4302533.2516619/94‑1 (MQ=60)

                                                                                    CCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATATT        <  SRR4302533.3379372/94‑1 (MQ=60)

                                                                                    CCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATATT        >  SRR4302533.3931034/1‑94 (MQ=60)

                                                                                    CCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATATT        <  SRR4302533.4036271/94‑1 (MQ=60)

                                                                                     CTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATATTG       <  SRR4302533.3905185/94‑1 (MQ=60)

                                                                                     CTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATATTG       >  SRR4302533.1395303/1‑94 (MQ=60)

                                                                                       tgTTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATATTGT      <  SRR4302533.3006766/92‑1 (MQ=60)

                                                                                       tgTTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATATTGT      >  SRR4302533.6156328/3‑94 (MQ=60)

                                                                                       tgTTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATATTGT      >  SRR4302533.5724367/3‑94 (MQ=60)

                                                                                        GTTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATATTGTC     >  SRR4302533.319775/1‑94 (MQ=60)

                                                                                        GTTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATATTGTC     >  SRR4302533.5693332/1‑94 (MQ=60)

                                                                                         TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATATTGTCC    <  SRR4302533.1508555/94‑1 (MQ=60)

                                                                                         TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCATTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATATTGTCC    >  SRR4302533.2685855/1‑94 (MQ=60)

                                                                                          TTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATATTGTCCT   <  SRR4302533.3642216/94‑1 (MQ=60)

                                                                                          TTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATATTGTCCT   >  SRR4302533.4541916/1‑94 (MQ=60)

                                                                                           TCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCTCAATATTGTCCTG  <  SRR4302533.1596407/94‑1 (MQ=60)

CCGGATTTTCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTGTTTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATATTGTCCTG  >  NC_000913/3393015‑3393199

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 7 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`