Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,405,469	T→C	W76R (TGG→CGG)	b3258 →	sodium/pantothenate symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,405,469	0	T	C	99.4%	526.8 / NA	168	W76R (TGG→CGG)	b3258	sodium/pantothenate symporter
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (56/111); minor base G (0/1); total (56/112)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 213 total aligned reads displayed.

ATTGTGCTGGCGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCTGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCGCGCCGCTAC  >  NC_000913/3405379‑3405558

   gtgCTGGCGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTa                                                                                      <  2:4104058/93‑1 (MQ=255)

   gtgCTGGCGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTa                                                                                      <  1:6290515/93‑1 (MQ=255)

   gtgCTGGCGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTa                                                                                      <  2:4039286/93‑1 (MQ=255)

    tgCTGGCGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTAt                                                                                     <  2:3103787/93‑1 (MQ=255)

    tgCTGGCGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTAt                                                                                     <  1:615075/93‑1 (MQ=255)

    tgCTGGCGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGGAt                                                                                     >  1:3609164/1‑93 (MQ=255)

      cTGGCGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTg                                                                                   <  2:2246545/93‑1 (MQ=255)

       tGGCGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGc                                                                                  <  1:49682/93‑1 (MQ=255)

        ggCGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCt                                                                                 <  2:1465466/93‑1 (MQ=255)

          cGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGCGCACGAGCGGCTTATTAATACAGGGTGGGGCGGGGATtggtgg                                                                               >  2:171053/1‑93 (MQ=255)

           gATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGc                                                                              <  2:3921061/93‑1 (MQ=255)

           gATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGc                                                                              <  1:4101756/93‑1 (MQ=255)

           gATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGGATTGCTggg                                                                              >  1:1423524/1‑92 (MQ=255)

              gACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGGCCGGGATTTCTGGCggt                                                                           >  2:5552366/1‑91 (MQ=255)

               aCGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCgatg                                                                          <  2:3545078/93‑1 (MQ=255)

                    cACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCa                                                                     <  2:1113329/93‑1 (MQ=255)

                    cACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGGATTGCTGGCGATGATTCa                                                                     >  2:1861678/1‑93 (MQ=255)

                     aCCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGGATTGCTGGCGGTGATTCAg                                                                    >  2:2991424/1‑93 (MQ=255)

                       cgcgACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCt                                                                  <  1:2305034/93‑1 (MQ=255)

                          gACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGGATTGCTGGGGGTGATTTAGCtttc                                                               >  2:4742245/1‑91 (MQ=255)

                          gACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGGATTGCTGGCGATGATTTAGCTTcc                                                               >  1:577809/1‑93 (MQ=255)

                           aCCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGGGCTGCTTATAAATACGGGCTGGGCCGGGGATTGCTGGCGATGATTCAGCTTCCt                                                              >  1:854123/1‑93 (MQ=255)

                           aCCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGGCGGGGATTGCTGGCGATGATTCAGCTTCCt                                                              >  1:3722732/1‑93 (MQ=255)

                             cTATATCAGTGCGAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGc                                                            <  2:41979/93‑1 (MQ=255)

                             cTACATCAGTGCCAGTTCGTTCATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGc                                                            <  2:1189348/93‑1 (MQ=255)

                              tataTCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCa                                                           <  1:6181003/93‑1 (MQ=255)

                              tataTCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCa                                                           <  1:5144955/93‑1 (MQ=255)

                              tataTCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGGATTGCTGGCGGTGATTCAGCTTCCTGCa                                                           >  2:6078120/1‑93 (MQ=255)

                              tataTCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGGATTGCTGGCGATGATTCAGCTTCCTGCa                                                           >  1:4603306/1‑93 (MQ=255)

                               atatCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAg                                                          <  1:4297800/93‑1 (MQ=255)

                                tatCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGt                                                         <  1:5574331/93‑1 (MQ=255)

                                tatCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGGATTGGTTGCGATGATTTATGTTGCTGCatt                                                         >  2:4906473/1‑91 (MQ=255)

                                tatCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGGATTGCTGGCGATGATTCAGCTTTCTGCAGt                                                         >  1:3351677/1‑93 (MQ=255)

                                 atCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGGCGGGGATTGCTGGCGATGATTCAGCTTCCTGCAGTc                                                        >  1:4676184/1‑93 (MQ=255)

                                 atCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTc                                                        <  2:4429146/93‑1 (MQ=255)

                                 atCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTc                                                        <  1:3944274/93‑1 (MQ=255)

                                  tCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGTGATGATTCAGCTTCCTGCAGTCt                                                       >  1:3140355/1‑93 (MQ=255)

                                  tCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGGATTGCTGGGGATGATTCAGCTTCCTGCAGTCt                                                       >  2:4994669/1‑93 (MQ=255)

                                   cAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTg                                                      <  1:4834126/93‑1 (MQ=255)

                                   cAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGGATTGCTGGCGATGATTCAGCTTCCTGCAGTCTg                                                      >  1:3697259/1‑93 (MQ=255)

                                     gTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGc                                                    <  2:3163731/93‑1 (MQ=255)

                                     gTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGc                                                    <  1:2127710/93‑1 (MQ=255)

                                      tGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTTGCt                                                   >  2:2835007/1‑93 (MQ=255)

                                        ccAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCttt                                                 <  2:2942203/93‑1 (MQ=255)

                                        ccAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGGATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCttt                                                 >  1:13058/1‑93 (MQ=255)

                                         cAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGGCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTc                                                >  2:2599960/1‑93 (MQ=255)

                                          aGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGGCGGGTATTGCTGGCGATGATTCAGGTTCCTGCAGTCTGGCCTTCa                                               >  1:5196770/1‑93 (MQ=255)

                                           gTTCTTTTAGCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCGGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCAc                                              <  2:3244484/93‑1 (MQ=255)

                                           gTTCGTTTATCGGCGGGCCAGGAGGTGCTTATAAATACTGGCTGGGCCGGGGATTGCTGGCGATGAATCAGCTTCCTGCAGTCTGGCTTTCAc                                              >  2:1326351/1‑93 (MQ=255)

                                           gTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCAc                                              >  1:4154066/1‑93 (MQ=255)

                                            ttCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACt                                             >  1:3108568/1‑93 (MQ=255)

                                            ttCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACt                                             <  2:5930493/93‑1 (MQ=255)

                                             tCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTc                                            <  1:4409195/93‑1 (MQ=255)

                                               gTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCgg                                          >  1:5483529/1‑93 (MQ=255)

                                                tttATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATCATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGt                                         <  1:5934731/93‑1 (MQ=255)

                                                 ttATCGGCGGGCCAGGAGCTGCTTATAAATACGGTCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTa                                        <  2:4518313/93‑1 (MQ=255)

                                                   aTCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGGTGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTAtt                                      >  2:2072529/1‑93 (MQ=255)

                                                   aTCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTAtt                                      <  1:1149708/93‑1 (MQ=255)

                                                     cggcggGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATtct                                    <  2:1257300/93‑1 (MQ=255)

                                                            ccAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCaag                             >  1:1400178/1‑93 (MQ=255)

                                                             cAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCaaga                            <  1:1997146/93‑1 (MQ=255)

                                                             cAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCaaga                            >  1:3082812/1‑93 (MQ=255)

                                                             cAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCaaga                            >  2:1907756/1‑93 (MQ=255)

                                                              aGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCaagaa                           >  1:234191/1‑93 (MQ=255)

                                                               gaaGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCaagaag                          >  1:5294673/3‑93 (MQ=255)

                                                                gAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCTGCAAGAAGt                         >  2:1792209/1‑93 (MQ=255)

                                                                gAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGt                         <  1:1119770/93‑1 (MQ=255)

                                                                gAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGt                         <  1:2474617/93‑1 (MQ=255)

                                                                gAGCTGCTGATAAATATGGGCTGGGCCGCGTATTGCTGACAATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAAAAGt                         <  2:4870881/93‑1 (MQ=255)

                                                                  gctgctTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGttt                       <  2:6291440/93‑1 (MQ=255)

                                                                  gctgctTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGttt                       >  2:6104524/1‑93 (MQ=255)

                                                                   ctgctTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTg                      <  1:5917485/93‑1 (MQ=255)

                                                                   ctgctTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTg                      <  2:5109963/93‑1 (MQ=255)

                                                                   ctgctTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTg                      <  1:377062/93‑1 (MQ=255)

                                                                   ctgctTATAAAGACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTg                      <  2:1564972/93‑1 (MQ=255)

                                                                    tgctTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGc                     >  2:1976342/1‑93 (MQ=255)

                                                                    tgctTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGc                     <  2:5725440/93‑1 (MQ=255)

                                                                    tgctTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGc                     >  1:4721403/1‑93 (MQ=255)

                                                                    tgctTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGc                     <  1:686394/93‑1 (MQ=255)

                                                                    tgctTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGc                     <  1:599649/93‑1 (MQ=255)

                                                                      ctTATAAATACGGGCTGGGCCGGGTATTGCGGGCGATGATTCAGCTTCCTGCAGTCTGCCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGa                   <  1:1032156/93‑1 (MQ=255)

                                                                       ttATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGAt                  <  1:5101726/93‑1 (MQ=255)

                                                                       ttATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGAt                  <  2:3986437/93‑1 (MQ=255)

                                                                       ttATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGAt                  <  1:3219028/93‑1 (MQ=255)

                                                                          taAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCt               >  1:3024759/1‑93 (MQ=255)

                                                                           aaaTACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCtt              <  1:5809543/93‑1 (MQ=255)

                                                                            aaTACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTg             >  2:4003813/1‑93 (MQ=255)

                                                                             aTACGGGCTGGGCCGGGTATTGCTTGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTgc            <  2:5251849/93‑1 (MQ=255)

                                                                             aTACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTgc            <  1:2894924/93‑1 (MQ=255)

                                                                             aTACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTgc            <  2:5781407/93‑1 (MQ=255)

                                                                             aTACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTgc            <  1:2141597/93‑1 (MQ=255)

                                                                             aTACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCCGTATTCTCGGCCAGAAGTTTGCGATTCTTgc            >  1:1501950/1‑93 (MQ=255)

                                                                               aCGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTgcgc          >  2:2126776/1‑93 (MQ=255)

                                                                                  ggctgggccgGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCGcgcc       >  2:3954078/1‑93 (MQ=255)

                                                                                   gctgggccggGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCGcgccg      <  1:1766862/93‑1 (MQ=255)

                                                                                   gctgggccggGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCGcgccg      >  1:5340959/1‑93 (MQ=255)

                                                                                    ctgggccgggTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCCCTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCGcgccgc     <  1:3389372/93‑1 (MQ=255)

                                                                                      gggccgggTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCGCGCCGCTa   >  1:5461658/1‑93 (MQ=255)

                                                                                       ggccgggTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCGCGCCGCTAc  >  1:1600957/1‑93 (MQ=255)

                                                                                       ggccgggTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCGCGCCGCTAc  <  2:3499229/93‑1 (MQ=255)

ATTGTGCTGGCGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCTGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCGCGCCGCTAC  >  NC_000913/3405379‑3405558

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GGTATTGTGCTGGCGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCTGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCGCGCCGCTACAATG  >  NC_000913/3405376‑3405562

GGTATTGTGCTGGCGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCCGGAGCTGCTTATAAATAAGGGCTGGGCG                                                                                               >  SRR4302533.4154763/1‑94 (MQ=60)

    TTGTGCTGGCGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGT                                                                                           <  SRR4302533.5371566/94‑1 (MQ=60)

      GTGCTGGCGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTAT                                                                                         <  SRR4302533.2424583/94‑1 (MQ=60)

      GTGCTGGCGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTAT                                                                                         <  SRR4302533.6328028/94‑1 (MQ=60)

      GTGCTGGCGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGGAT                                                                                         >  SRR4302533.3631380/1‑94 (MQ=60)

       TGCTGGCGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATT                                                                                        <  SRR4302533.618689/94‑1 (MQ=60)

        GCTGGCGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGATTATTAATACCGGCTGGGCCGGGGATTG                                                                                       >  SRR4302533.603231/1‑94 (MQ=60)

          TGGCGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCT                                                                                     <  SRR4302533.50024/94‑1 (MQ=60)

          TGGCGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCT                                                                                     <  SRR4302533.1098843/94‑1 (MQ=60)

             CGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGC                                                                                  <  SRR4302533.597226/94‑1 (MQ=60)

             CGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGGATTGCTGGG                                                                                  >  SRR4302533.1432135/1‑94 (MQ=60)

              GATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCG                                                                                 <  SRR4302533.4126715/94‑1 (MQ=60)

                TGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGAT                                                                               <  SRR4302533.4716650/94‑1 (MQ=60)

                 GACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATG                                                                              <  SRR4302533.1137346/94‑1 (MQ=60)

                 GACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGGATTGCTGGCGATG                                                                              >  SRR4302533.2221952/1‑94 (MQ=60)

                  ACGCTCACCGCGACCTATATCAGTGCCATTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGA                                                                             <  SRR4302533.4837663/94‑1 (MQ=60)

                  ACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGA                                                                             <  SRR4302533.3384034/94‑1 (MQ=60)

                   CGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCACGAGCTGCTTATAAAAACGGGCTGGGCCGGGGATTTCTGGCGGTGAT                                                                            >  SRR4302533.5173911/1‑94 (MQ=60)

                      TCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCA                                                                         <  SRR4302533.3768214/94‑1 (MQ=60)

                      TCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGAGGCTTCTAAAAACGGCGCGGGCCGGGTATTGCCGGCGATGCTTCA                                                                         >  SRR4302533.3123353/1‑94 (MQ=50)

                       CACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAG                                                                        <  SRR4302533.3340833/94‑1 (MQ=60)

                        CCCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGC                                                                       <  SRR4302533.520140/94‑1 (MQ=60)

                          CGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTT                                                                     <  SRR4302533.2319311/94‑1 (MQ=60)

                            CGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCC                                                                   <  SRR4302533.487279/94‑1 (MQ=60)

                            CGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGGATTGCTGGCGATGATTTAGCTTCC                                                                   >  SRR4302533.581215/1‑94 (MQ=60)

                             GACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGGGCTGCTTATAAATACGGGCTGGGCCGGGGATTGCTGGCGATGATTCAGCTTCCT                                                                  >  SRR4302533.859127/1‑94 (MQ=60)

                             GACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGGCGGGGATTGCTGGCGATGATTCAGCTTCCT                                                                  >  SRR4302533.3745575/1‑94 (MQ=60)

                             GACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATAAGGGCTGGGCCGGGGATTGCTGGCGATGATTCAGGTTTCT                                                                  >  SRR4302533.440668/1‑94 (MQ=60)

                              ACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTG                                                                 <  SRR4302533.5244215/94‑1 (MQ=60)

                               CCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGGGCTGCTTATAAAAACGGGCTGGGCCGGGGATTGCTGGCGATGATTCAGCTTCCTTC                                                                >  SRR4302533.5507715/1‑94 (MQ=60)

                               CCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGGATTGCTGGGGATGATTCAGCTTCCTGC                                                                >  SRR4302533.60628/1‑94 (MQ=60)

                                CTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCA                                                               <  SRR4302533.4002983/94‑1 (MQ=60)

                                CTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGGATTGCTGGCGATGATTCAGCTTCCTGCA                                                               >  SRR4302533.4631192/1‑94 (MQ=60)

                                 TATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAG                                                              <  SRR4302533.5176046/94‑1 (MQ=60)

                                 TATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAG                                                              <  SRR4302533.5555565/94‑1 (MQ=60)

                                 TATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAG                                                              <  SRR4302533.6218123/94‑1 (MQ=60)

                                  ATATCAGTGCCAGTTCGTTTATCGGCGGGGCAGGAGCTGCTTATTAATACGGGCTGGGCCGGGTATTGCTGGCGGTGATTCAGCTTTCCGCAGT                                                             >  SRR4302533.5817642/1‑94 (MQ=60)

                                  ATATCAGTGCCAGTTCGTTTATCGGCGGGCCCGGAGCTGCTTATAAATACGGGCTGGGCCGGGTAGTGCTGGCGGTGGTTCAGCTTCCTGCCGT                                                             >  SRR4302533.2289967/1‑94 (MQ=60)

                                  ATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGT                                                             <  SRR4302533.4323646/94‑1 (MQ=60)

                                  ATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGGATTGCTGGCGATGATTCAGCTTTCTGCAGT                                                             >  SRR4302533.3372381/1‑94 (MQ=60)

                                   TATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGGGCTGCTTATAAATACGGGCTGGGCCGGGGATTGCTGGCGATGATTTAGCTTTCTGCAGTT                                                            >  SRR4302533.4326170/1‑94 (MQ=60)

                                   TATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGGCGGGGATTGCTGGCGATGATTCAGCTTCCTGCAGTC                                                            >  SRR4302533.4704526/1‑94 (MQ=60)

                                   TATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTC                                                            <  SRR4302533.5608019/94‑1 (MQ=60)

                                   TATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGGATTGCTGGCGATGATTTAGCTTCCTGCAGTC                                                            >  SRR4302533.5482642/1‑94 (MQ=60)

                                    ATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGTGATGATTCAGCTTCCTGCAGTCT                                                           >  SRR4302533.3159661/1‑94 (MQ=60)

                                    ATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCT                                                           <  SRR4302533.3968335/94‑1 (MQ=60)

                                     TCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTG                                                          <  SRR4302533.2960214/94‑1 (MQ=60)

                                     TCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGGATTGCTGGCGATGATTCAGCTTCCTGCAGTCTG                                                          >  SRR4302533.3719971/1‑94 (MQ=60)

                                      CAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGG                                                         <  SRR4302533.1507584/94‑1 (MQ=60)

                                      CAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGG                                                         <  SRR4302533.4863427/94‑1 (MQ=60)

                                       AGTGCCAGTTCGTTTATCGGCGGGCCAGAAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGC                                                        <  SRR4302533.1171884/94‑1 (MQ=60)

                                        GTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCT                                                       <  SRR4302533.2140831/94‑1 (MQ=60)

                                         TGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTT                                                      <  SRR4302533.3944356/94‑1 (MQ=60)

                                          GCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATT‑AGCTTCCTGCAGTCTGGCTTTC                                                    <  SRR4302533.6233787/94‑1 (MQ=60)

                                          GCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGGATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTT                                                     >  SRR4302533.13158/1‑94 (MQ=60)

                                            CAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGGCGGGTATTGCTGGCGATGATTCAGGTTCCTGCAGTCTGGCCTTCA                                                   >  SRR4302533.5228109/1‑94 (MQ=60)

                                            CAGTTCGTTTATCGGCGGGCCAGGAGCTGCCTATAAATACCGGCTGGGCCGGGGATTGCTGGCGATGATTTAGCTTCCTGCCGTCTGGCTTTtc                                                   >  SRR4302533.141525/1‑92 (MQ=60)

                                             AGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCAC                                                  >  SRR4302533.4179237/1‑94 (MQ=60)

                                              GTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACT                                                 >  SRR4302533.3127673/1‑94 (MQ=60)

                                               TTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTC                                                <  SRR4302533.3632883/94‑1 (MQ=60)

                                                TCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCG                                               <  SRR4302533.5084940/94‑1 (MQ=60)

                                                TCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCG                                               <  SRR4302533.4435821/94‑1 (MQ=60)

                                                 CGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGG                                              >  SRR4302533.5516581/1‑94 (MQ=60)

                                                   TTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATCATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTA                                            <  SRR4302533.5970609/94‑1 (MQ=60)

                                                     TATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATT                                          <  SRR4302533.4449335/94‑1 (MQ=60)

                                                      ATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTC                                         <  SRR4302533.3740387/94‑1 (MQ=60)

                                                      ATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTC                                         <  SRR4302533.1156575/94‑1 (MQ=60)

                                                        CGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTC                                       <  SRR4302533.985538/94‑1 (MQ=60)

                                                        CGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGGATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTTTC                                       >  SRR4302533.1150446/1‑94 (MQ=60)

                                                              GCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAG                                 >  SRR4302533.1408618/1‑94 (MQ=60)

                                                              GCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAG                                 <  SRR4302533.2741957/94‑1 (MQ=60)

                                                               CCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGA                                >  SRR4302533.3482984/1‑94 (MQ=60)

                                                               CCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGA                                >  SRR4302533.3101764/1‑94 (MQ=60)

                                                                CAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAA                               >  SRR4302533.1758921/1‑94 (MQ=60)

                                                                CAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAA                               <  SRR4302533.2009431/94‑1 (MQ=60)

                                                                CAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAA                               >  SRR4302533.5954161/1‑94 (MQ=60)

                                                                CAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAA                               >  SRR4302533.235638/1‑94 (MQ=60)

                                                                 AGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGTAAGAAG                              >  SRR4302533.708214/1‑94 (MQ=60)

                                                                 AGAAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAG                              >  SRR4302533.5326378/1‑94 (MQ=60)

                                                                   GAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTT                            <  SRR4302533.1126413/94‑1 (MQ=60)

                                                                   GAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTT                            <  SRR4302533.2489876/94‑1 (MQ=60)

                                                                    AGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTT                           <  SRR4302533.642432/94‑1 (MQ=60)

                                                                    AGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTT                           <  SRR4302533.1382977/94‑1 (MQ=60)

                                                                    AGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTT                           <  SRR4302533.5912906/94‑1 (MQ=60)

                                                                      CTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGC                         >  SRR4302533.4750030/1‑94 (MQ=60)

                                                                      CTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGC                         <  SRR4302533.379335/94‑1 (MQ=60)

                                                                      CTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGC                         <  SRR4302533.5876404/94‑1 (MQ=60)

                                                                      CTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGC                         <  SRR4302533.5953245/94‑1 (MQ=60)

                                                                       TGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCG                        <  SRR4302533.603177/94‑1 (MQ=60)

                                                                       TGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCG                        >  SRR4302533.215009/1‑94 (MQ=60)

                                                                       TGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCG                        <  SRR4302533.690453/94‑1 (MQ=60)

                                                                        GCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGA                       >  SRR4302533.4354122/1‑94 (MQ=60)

                                                                        GCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGA                       >  SRR4302533.4150166/1‑94 (MQ=60)

                                                                         CTTATAAATACGGGCTGGGCCGGGTATTGCGGGCGATGATTCAGCTTCCTGCAGTCTGCCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGAT                      <  SRR4302533.1038224/94‑1 (MQ=60)

                                                                         CTTATAAAGACGGGCTGGATCGGGTATTGCTGGCGATGAGTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGAT                      <  SRR4302533.3131506/94‑1 (MQ=60)

                                                                          TTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATT                     >  SRR4302533.2952957/1‑94 (MQ=60)

                                                                          TTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATT                     <  SRR4302533.5132542/94‑1 (MQ=60)

                                                                          TTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATT                     <  SRR4302533.3238877/94‑1 (MQ=60)

                                                                          TTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATT                     >  SRR4302533.5220923/1‑94 (MQ=60)

                                                                          TTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATT                     <  SRR4302533.1922345/94‑1 (MQ=60)

                                                                          TTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATT                     >  SRR4302533.5839987/1‑94 (MQ=60)

                                                                           TATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTC                    >  SRR4302533.2096321/1‑94 (MQ=60)

                                                                           TATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTC                    >  SRR4302533.732269/1‑94 (MQ=60)

                                                                           TATAAATACGGGCTGGGCCGGGTATGGCGGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTC                    <  SRR4302533.4695836/94‑1 (MQ=60)

                                                                            ATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCT                   >  SRR4302533.3043264/1‑94 (MQ=60)

                                                                              AAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTG                 <  SRR4302533.2332456/94‑1 (MQ=60)

                                                                              AAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTG                 <  SRR4302533.5844626/94‑1 (MQ=60)

                                                                              AAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTG                 <  SRR4302533.1607373/94‑1 (MQ=60)

                                                                               AATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCCGTATTCTCGGCCAGAAGTTTGCGATTCTTGC                >  SRR4302533.1511004/1‑94 (MQ=60)

                                                                                ATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCG               <  SRR4302533.2912711/94‑1 (MQ=60)

                                                                                ATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCG               <  SRR4302533.3179793/94‑1 (MQ=60)

                                                                                ATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCG               <  SRR4302533.1418917/94‑1 (MQ=60)

                                                                                ATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCG               <  SRR4302533.2154795/94‑1 (MQ=60)

                                                                                  ACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCGCG             >  SRR4302533.5734690/1‑94 (MQ=60)

                                                                                   CGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGCATTCTCGGCTAGAAGTTTGCGATTCTTGCGCGC            >  SRR4302533.4194757/1‑94 (MQ=60)

                                                                                    GGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCGCGCC           >  SRR4302533.3060451/1‑94 (MQ=60)

                                                                                     GGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCGCGCCG          >  SRR4302533.5372988/1‑94 (MQ=60)

                                                                                      GCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCGCGCCGC         <  SRR4302533.1777620/94‑1 (MQ=60)

                                                                                       CTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCCCTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCGCGCCGCT        <  SRR4302533.3410303/94‑1 (MQ=60)

                                                                                        TGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCGCGCCGCTA       >  SRR4302533.5494563/1‑94 (MQ=60)

                                                                                         GGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCGCGCCGCTAC      >  SRR4302533.1610647/1‑94 (MQ=60)

                                                                                         GGGCCGGGAATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCGCGCCGCTAC      >  SRR4302533.1666418/1‑94 (MQ=60)

                                                                                          GGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCGCGCCGCTACA     <  SRR4302533.6215803/94‑1 (MQ=60)

                                                                                           GCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCGCGCCGCTACAA    >  SRR4302533.5738154/1‑94 (MQ=60)

                                                                                            CCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCGCGCCGCTACAAT   >  SRR4302533.5916282/1‑94 (MQ=60)

                                                                                            CCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCGCGCCGCTACAAT   <  SRR4302533.3780127/94‑1 (MQ=60)

                                                                                            CCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCGCGCCGCTACAAT   <  SRR4302533.5022807/94‑1 (MQ=60)

                                                                                            CCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCGCGCCGCTACAAT   >  SRR4302533.2568789/1‑94 (MQ=60)

                                                                                             CGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCGCGCCGCTACAATG  >  SRR4302533.319012/1‑94 (MQ=60)

                                                                                             CGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCGCGCCGCTACAATG  <  SRR4302533.3716150/94‑1 (MQ=60)

                                                                                             CGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCGCGCCGCTACAATG  >  SRR4302533.1110022/1‑94 (MQ=60)

GGTATTGTGCTGGCGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCTGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCGCGCCGCTACAATG  >  NC_000913/3405376‑3405562

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`