Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,434,055 A→G P404P (CCA→CCG)  sun → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,434,0550AG100.0% 733.1 / NA 226P404P (CCA→CCG) sunhypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (128/98);  total (128/98)

Only 100 of 233 total aligned reads displayed.

TGTTCGGTGTTACCGGAAGAGAATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCAGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGTGATGAGATAACGG  >  NC_000913/3433966‑3434144
                                                                                         |                                                                                         
tGTTCGGTGTTACCGGAAGAGAATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCggag                                                                                        >  2:5079634/1‑93 (MQ=255)
   tCGGTGTTACCGGAAGAGAATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCaa                                                                                     >  1:5911475/1‑93 (MQ=255)
   tCGGTGTTACCGGAAGAGAATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCaa                                                                                     >  2:1015132/1‑93 (MQ=255)
    cGGTGTTACCGGAAGAGAATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAAc                                                                                    >  1:35974/1‑93 (MQ=255)
     ggTGTTACCGGAAGAGAATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAAcc                                                                                   >  1:155525/1‑93 (MQ=255)
        gtTACCGGAAGAGAATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCggg                                                                                >  1:5103167/1‑93 (MQ=255)
         ttACCGGAAGAGAATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGt                                                                               <  2:3488207/93‑1 (MQ=255)
          tACCGGAAGAGAATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTa                                                                              <  2:766048/93‑1 (MQ=255)
            ccGGAAGAGAATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTaaa                                                                            <  2:6042496/93‑1 (MQ=255)
             cagAAGAGAATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGACCTTTGCGAAACAGGAACACCGGAGAAACCGGGTAAAc                                                                           <  1:1856908/91‑1 (MQ=255)
              ggAAGAGAATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACa                                                                          >  1:2842887/1‑93 (MQ=255)
              ggAAGAGAATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACa                                                                          >  1:72610/1‑93 (MQ=255)
              ggAAGAGAATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACa                                                                          >  1:3456532/1‑93 (MQ=255)
                aaGAGAATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACaaa                                                                        <  1:4299189/93‑1 (MQ=255)
                aaGAGAATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACaaa                                                                        >  1:2662816/1‑93 (MQ=255)
                  gagaATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAGCTTTGCGAAACAGGAACACCGGAGCAGCCGGGTAAACAAAAt                                                                      <  2:4279426/93‑1 (MQ=255)
                  gagaATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAAt                                                                      >  2:227935/1‑93 (MQ=255)
                  gagaATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAAt                                                                      >  1:3745586/1‑93 (MQ=255)
                  gagaATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAAt                                                                      <  2:6212793/93‑1 (MQ=255)
                  gagaATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAAt                                                                      <  1:2510934/93‑1 (MQ=255)
                   agaATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATc                                                                     <  1:2111161/93‑1 (MQ=255)
                    gaATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCt                                                                    >  1:1820216/1‑93 (MQ=255)
                    gaATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACAGGAGCAACCGGGTAAACAAAATCt                                                                    >  1:2092025/1‑93 (MQ=255)
                      aTAGCCTGCAGATTAAAGGCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGACACAGGAACAGCAGAGCAACCGGATAAACAAAATCTAc                                                                  >  1:3344101/1‑93 (MQ=255)
                      aTAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTAc                                                                  >  2:1809251/1‑93 (MQ=255)
                      aTAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTAc                                                                  <  2:2445242/93‑1 (MQ=255)
                       tAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGATCTTTGCGAACCAGGACCCCCGGAGCACCCGGGTAAACAAAATCTAcc                                                                 <  1:1176707/93‑1 (MQ=255)
                       tAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGATCTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTAcc                                                                 <  2:4301752/93‑1 (MQ=255)
                       tAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTAcc                                                                 >  1:541484/1‑93 (MQ=255)
                        aGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCt                                                                >  2:4596057/1‑93 (MQ=255)
                         gCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTg                                                               <  2:3910998/93‑1 (MQ=255)
                         gCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTg                                                               <  2:1851316/93‑1 (MQ=255)
                           cTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTggg                                                             >  1:3933911/1‑92 (MQ=255)
                           cTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGt                                                             <  2:5289534/93‑1 (MQ=255)
                           cTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGt                                                             >  2:1276750/1‑93 (MQ=255)
                           cTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGt                                                             <  1:4641640/93‑1 (MQ=255)
                             gCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGc                                                           >  2:1398169/1‑93 (MQ=255)
                             gCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGc                                                           >  1:681896/1‑93 (MQ=255)
                             gCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGc                                                           >  2:3022018/1‑93 (MQ=255)
                                  ttAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAag                                                      >  1:492043/1‑93 (MQ=255)
                                   tAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAaga                                                     >  1:1812016/1‑93 (MQ=255)
                                    aaaGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAagag                                                    >  1:2580292/1‑93 (MQ=255)
                                    aaaGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAagag                                                    >  1:5594633/1‑93 (MQ=255)
                                     aaGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAgg                                                   >  2:3309644/1‑93 (MQ=255)
                                     aaGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAgg                                                   >  1:3006685/1‑93 (MQ=255)
                                        ccTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCg                                                >  2:2564591/1‑93 (MQ=255)
                                         cTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGa                                               <  1:1912508/93‑1 (MQ=255)
                                         cTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGa                                               >  1:1365641/1‑93 (MQ=255)
                                          tttttGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGAc                                              >  2:856243/1‑93 (MQ=255)
                                           ttttGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACg                                             <  1:106967/93‑1 (MQ=255)
                                             ttGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGc                                           <  1:5003671/93‑1 (MQ=255)
                                             ttGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGc                                           <  2:729656/93‑1 (MQ=255)
                                              tGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGCGGGCGACGGct                                          >  2:5234535/1‑93 (MQ=255)
                                               gCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGctt                                         <  1:5324047/93‑1 (MQ=255)
                                                 aaCGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGcttct                                       <  2:3593609/93‑1 (MQ=255)
                                                  aCGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGcttctt                                      <  1:2273486/93‑1 (MQ=255)
                                                   cGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCttt                                     >  2:5914126/1‑93 (MQ=255)
                                                     tACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTa                                   >  1:1804170/1‑93 (MQ=255)
                                                     tACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTa                                   >  2:917377/1‑93 (MQ=255)
                                                     tACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTa                                   >  1:2125865/1‑93 (MQ=255)
                                                        cGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACgc                                >  1:4146509/1‑93 (MQ=255)
                                                        cGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACgc                                >  1:4395045/1‑93 (MQ=255)
                                                         gCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACgct                               >  2:4967937/1‑93 (MQ=255)
                                                          cTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACgcta                              >  2:4039926/1‑93 (MQ=255)
                                                           tGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACgctaa                             <  1:2347464/93‑1 (MQ=255)
                                                           tGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACgctaa                             <  2:678914/93‑1 (MQ=255)
                                                            gATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACgctaag                            <  1:1503471/93‑1 (MQ=255)
                                                            gATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACgctaag                            <  1:5667862/93‑1 (MQ=255)
                                                            gATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACgctaag                            >  2:3397616/1‑93 (MQ=255)
                                                            gATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACgctaag                            >  1:1214261/1‑93 (MQ=255)
                                                             aTGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACgctaagc                           <  1:1269694/93‑1 (MQ=255)
                                                                ccGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACgctaagctaa                        <  2:1593892/93‑1 (MQ=255)
                                                                ccGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACgctaagctaa                        <  1:5161390/93‑1 (MQ=255)
                                                                  gAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATc                      <  1:5806167/93‑1 (MQ=255)
                                                                    aCTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCaa                    >  1:356540/1‑93 (MQ=255)
                                                                     cTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCaaa                   >  1:753447/1‑93 (MQ=255)
                                                                      tttGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCaaaa                  <  2:2849053/93‑1 (MQ=255)
                                                                       ttGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCaaaaa                 <  1:3080249/93‑1 (MQ=255)
                                                                       ttGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCaaaaa                 >  2:5491731/1‑93 (MQ=255)
                                                                         gCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGt               <  1:6166115/93‑1 (MQ=255)
                                                                           gAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGtga             >  1:647458/1‑93 (MQ=255)
                                                                           gAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGtga             <  1:2382506/93‑1 (MQ=255)
                                                                           gAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGtga             >  2:3953295/1‑93 (MQ=255)
                                                                            aaaCAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGtgat            <  2:1929123/93‑1 (MQ=255)
                                                                            aaaCAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGtgat            <  2:4222752/93‑1 (MQ=255)
                                                                            aaaCAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGtgat            >  1:5553721/1‑93 (MQ=255)
                                                                            aaaCAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGtgat            >  1:545996/1‑93 (MQ=255)
                                                                             aaCAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGtgatg           <  2:4463439/93‑1 (MQ=255)
                                                                             aaCAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGtgatg           >  1:941109/1‑93 (MQ=255)
                                                                              aCAGCAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCAGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGtgatga          <  2:5653556/93‑1 (MQ=255)
                                                                                aGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGTGATgaga        <  2:558435/93‑1 (MQ=255)
                                                                                 ggAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGTGATGAGAt       >  1:4018523/1‑93 (MQ=255)
                                                                                 ggAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGTGATGAGAt       <  2:2417775/93‑1 (MQ=255)
                                                                                  gAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGTGATGAGATa      <  2:4768978/93‑1 (MQ=255)
                                                                                   aaCACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGTGATGAGATaa     <  1:4173728/93‑1 (MQ=255)
                                                                                    acacCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGTGATGAGATAAc    <  2:3773895/93‑1 (MQ=255)
                                                                                     cacCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGTGATGAGATAACg   >  1:4061519/1‑93 (MQ=255)
                                                                                     cacCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGTGATGAGATAACg   <  1:2868270/93‑1 (MQ=255)
                                                                                      acCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGTGATGAGATAACgg  >  2:3843750/1‑93 (MQ=255)
                                                                                      acCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGTGATGAGATAACgg  >  1:1990200/1‑93 (MQ=255)
                                                                                         |                                                                                         
TGTTCGGTGTTACCGGAAGAGAATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCAGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGTGATGAGATAACGG  >  NC_000913/3433966‑3434144

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CACCTGTTCGGTGTTACCGGAAGAGAATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCAGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGTGATGAGATAACGGGTC  >  NC_000913/3433962‑3434147
                                                                                             |                                                                                            
CACCTGTTCGGTGTTACCGGAAGAGAATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCG                                                                                              >  SRR4302533.4785199/1‑94 (MQ=60)
CACCTGTTCGGTGTTACCGGAAGAGAATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCG                                                                                              >  SRR4302533.4932799/1‑94 (MQ=60)
 ACCTGTTCGGTGTTACCGGAAGAGAATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGG                                                                                             <  SRR4302533.4672787/94‑1 (MQ=60)
 ACCTGTTCGGTGTTACCGGAAGAGAATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGG                                                                                             <  SRR4302533.5602378/94‑1 (MQ=60)
 ACCTGTTCGGTGTTACCGGAAGAGAATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGG                                                                                             <  SRR4302533.4679365/94‑1 (MQ=60)
      TTCGGTGTTACCGGAAGAGAATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAA                                                                                        >  SRR4302533.4607771/1‑94 (MQ=60)
      TTCGGTGTTACCGGAAGAGAATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAA                                                                                        >  SRR4302533.5947190/1‑94 (MQ=60)
       TCGGTGTTACCGGAAGAGAATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAAC                                                                                       >  SRR4302533.36213/1‑94 (MQ=60)
        CGGTGTTACCGGAAGAGAATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACC                                                                                      >  SRR4302533.5516196/1‑94 (MQ=60)
        CGGTGTTACCGGAAGAGAATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACC                                                                                      >  SRR4302533.156515/1‑94 (MQ=60)
        CGGTGTTACCGGAAGAGAATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACC                                                                                      <  SRR4302533.5098168/94‑1 (MQ=60)
           TGTTACCGGAAGAGAATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGG                                                                                   >  SRR4302533.5133995/1‑94 (MQ=60)
                CCGGAAGAGAATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAAC                                                                              >  SRR4302533.743210/1‑94 (MQ=60)
                 CGGAAGAGAATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACA                                                                             >  SRR4302533.73077/1‑94 (MQ=60)
                 CGGAAGAGAATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACA                                                                             >  SRR4302533.2860383/1‑94 (MQ=60)
                 CGGAAGAGAATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACA                                                                             >  SRR4302533.174359/1‑94 (MQ=60)
                 CGGAAGAGAATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACA                                                                             >  SRR4302533.3477870/1‑94 (MQ=60)
                 CGGAAGAGAATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACA                                                                             <  SRR4302533.4249281/94‑1 (MQ=60)
                 CAGAAGAGAATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGACCTTTGCGAAACAGGAACACCGGAGAAACCGGGTAAACA                                                                             <  SRR4302533.1868235/94‑1 (MQ=60)
                   GAAGAGAATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAA                                                                           >  SRR4302533.2679166/1‑94 (MQ=60)
                    AAGAGAATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAA                                                                          <  SRR4302533.4325045/94‑1 (MQ=60)
                     AGAGAATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAAT                                                                         >  SRR4302533.3768554/1‑94 (MQ=60)
                      GAGAATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATC                                                                        >  SRR4302533.4857596/1‑94 (MQ=60)
                      GAGAATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATC                                                                        <  SRR4302533.2526407/94‑1 (MQ=60)
                      GAGAATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATC                                                                        >  SRR4302533.2181587/1‑94 (MQ=60)
                       AGAATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCTGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCT                                                                       >  SRR4302533.6251007/1‑94 (MQ=60)
                       AGAATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCT                                                                       >  SRR4302533.1831333/1‑94 (MQ=60)
                       AGAATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCT                                                                       <  SRR4302533.2124183/94‑1 (MQ=60)
                       AGAATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACAGGAGCAACCGGGTAAACAAAATCT                                                                       >  SRR4302533.2104908/1‑94 (MQ=60)
                        GAATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTA                                                                      <  SRR4302533.1198208/94‑1 (MQ=60)
                         AATAGCCTGCAGATTAAAGGCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGACACAGGAACAGCAGAGCAACCGGATAAACAAAATCTAC                                                                     >  SRR4302533.3364764/1‑94 (MQ=60)
                          ATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACC                                                                    >  SRR4302533.544704/1‑94 (MQ=60)
                          ATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACC                                                                    <  SRR4302533.760403/94‑1 (MQ=60)
                          ATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACC                                                                    <  SRR4302533.1894698/94‑1 (MQ=60)
                           TAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGATCTTTGCGAACCAGGACCCCCGGAGCACCCGGGTAAACAAAATCTACCG                                                                   <  SRR4302533.1183768/94‑1 (MQ=60)
                           TAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCT                                                                   <  SRR4302533.4074974/94‑1 (MQ=60)
                            AGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTG                                                                  <  SRR4302533.1332812/94‑1 (MQ=60)
                            AGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTG                                                                  <  SRR4302533.2425287/94‑1 (MQ=60)
                             GCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGG                                                                 <  SRR4302533.5818625/94‑1 (MQ=60)
                              CCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGT                                                                <  SRR4302533.3937805/94‑1 (MQ=60)
                              CCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGT                                                                <  SRR4302533.148778/94‑1 (MQ=60)
                              CCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGG                                                                >  SRR4302533.3957922/1‑94 (MQ=60)
                               CTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTG                                                               <  SRR4302533.4669762/94‑1 (MQ=60)
                                TGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGC                                                              >  SRR4302533.685928/1‑94 (MQ=60)
                                  CAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCG                                                            >  SRR4302533.3610274/1‑94 (MQ=60)
                                    GATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAA                                                          >  SRR4302533.1276947/1‑94 (MQ=60)
                                     ATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAG                                                         >  SRR4302533.1851528/1‑94 (MQ=60)
                                     ATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAG                                                         >  SRR4302533.494984/1‑94 (MQ=60)
                                     ATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAG                                                         <  SRR4302533.2043429/94‑1 (MQ=60)
                                      TTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGA                                                        >  SRR4302533.3470971/1‑94 (MQ=60)
                                      TTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGA                                                        >  SRR4302533.4127750/1‑94 (MQ=60)
                                      TTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGA                                                        <  SRR4302533.2106885/94‑1 (MQ=60)
                                      TTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGA                                                        >  SRR4302533.1988465/1‑94 (MQ=60)
                                      TTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGA                                                        >  SRR4302533.1823086/1‑94 (MQ=60)
                                       TAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAG                                                       >  SRR4302533.2596169/1‑94 (MQ=60)
                                       TAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAG                                                       <  SRR4302533.3644296/94‑1 (MQ=60)
                                       TAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAG                                                       >  SRR4302533.5628431/1‑94 (MQ=60)
                                        AAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGG                                                      >  SRR4302533.4355553/1‑94 (MQ=60)
                                        AAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGG                                                      <  SRR4302533.2880508/94‑1 (MQ=60)
                                        AAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGG                                                      >  SRR4302533.2002595/1‑94 (MQ=60)
                                        AAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGG                                                      >  SRR4302533.3025076/1‑94 (MQ=60)
                                        AAAGCCTATATGCAACGTACCGCTGATGCCGAACTTGGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGG                                                      <  SRR4302533.5682878/94‑1 (MQ=60)
                                         AAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGG                                                     >  SRR4302533.748210/1‑94 (MQ=60)
                                          AGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGC                                                    <  SRR4302533.958224/94‑1 (MQ=60)
                                          AGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGC                                                    >  SRR4302533.1819576/1‑94 (MQ=60)
                                            CCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGA                                                  >  SRR4302533.1373852/1‑94 (MQ=60)
                                             CTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGAG                                                 >  SRR4302533.5589585/1‑94 (MQ=60)
                                             CTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGAC                                                 >  SRR4302533.3507456/1‑94 (MQ=60)
                                             CTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGAC                                                 <  SRR4302533.1924176/94‑1 (MQ=60)
                                               TTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGG                                               <  SRR4302533.107636/94‑1 (MQ=60)
                                               TTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGG                                               <  SRR4302533.4198057/94‑1 (MQ=60)
                                                TTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGC                                              <  SRR4302533.5282196/94‑1 (MQ=60)
                                                 TTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCT                                             <  SRR4302533.5033996/94‑1 (MQ=60)
                                                 TTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCT                                             >  SRR4302533.2341499/1‑94 (MQ=60)
                                                  TGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTT                                            >  SRR4302533.1369887/1‑94 (MQ=60)
                                                   GCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTC                                           <  SRR4302533.5355955/94‑1 (MQ=60)
                                                    CAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCT                                          >  SRR4302533.5456537/1‑94 (MQ=60)
                                                    CAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCT                                          >  SRR4302533.3030337/1‑94 (MQ=60)
                                                    CAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCT                                          >  SRR4302533.5934072/1‑94 (MQ=60)
                                                    CAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCT                                          >  SRR4302533.2132849/1‑94 (MQ=60)
                                                     AACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTT                                         >  SRR4302533.1830095/1‑94 (MQ=60)
                                                     AACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTT                                         <  SRR4302533.2610010/94‑1 (MQ=60)
                                                      ACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTT                                        <  SRR4302533.2287558/94‑1 (MQ=60)
                                                       CGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTT                                       <  SRR4302533.4153661/94‑1 (MQ=60)
                                                        GTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTA                                      >  SRR4302533.1815150/1‑94 (MQ=60)
                                                        GTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTA                                      >  SRR4302533.4976952/1‑94 (MQ=60)
                                                        GTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTA                                      >  SRR4302533.2138971/1‑94 (MQ=60)
                                                         TACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTAC                                     >  SRR4302533.5425367/1‑94 (MQ=60)
                                                          ACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACG                                    <  SRR4302533.1865452/94‑1 (MQ=60)
                                                           CCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGC                                   >  SRR4302533.4171635/1‑94 (MQ=60)
                                                           CCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGC                                   <  SRR4302533.3017098/94‑1 (MQ=60)
                                                           CCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGC                                   >  SRR4302533.4421580/1‑94 (MQ=60)
                                                           CCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGC                                   >  SRR4302533.1519647/1‑94 (MQ=60)
                                                            CGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCT                                  >  SRR4302533.3560014/1‑94 (MQ=60)
                                                            CGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCT                                  <  SRR4302533.3982294/94‑1 (MQ=60)
                                                             GCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTA                                 >  SRR4302533.1136953/1‑94 (MQ=60)
                                                              CTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAA                                >  SRR4302533.4341042/1‑94 (MQ=60)
                                                              CTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAA                                <  SRR4302533.66702/94‑1 (MQ=60)
                                                              CTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAA                                >  SRR4302533.5173286/1‑94 (MQ=60)
                                                               TGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAG                               <  SRR4302533.2361988/94‑1 (MQ=60)
                                                               TGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAG                               <  SRR4302533.5837440/94‑1 (MQ=60)
                                                               TGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAG                               >  SRR4302533.3880561/1‑94 (MQ=60)
                                                               TGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAG                               >  SRR4302533.1221557/1‑94 (MQ=60)
                                                                GATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGC                              <  SRR4302533.5702099/94‑1 (MQ=60)
                                                                GATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGC                              <  SRR4302533.1512537/94‑1 (MQ=60)
                                                                 ATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCT                             <  SRR4302533.1277292/94‑1 (MQ=60)
                                                                 ATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCT                             <  SRR4302533.6074008/94‑1 (MQ=60)
                                                                   GCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAA                           >  SRR4302533.2441838/1‑94 (MQ=60)
                                                                    CCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAAT                          <  SRR4302533.5192551/94‑1 (MQ=60)
                                                                    CCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGCAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAAT                          >  SRR4302533.3029765/1‑94 (MQ=60)
                                                                     CGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATC                         >  SRR4302533.2395973/1‑94 (MQ=60)
                                                                      GAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGTAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCA                        >  SRR4302533.4994352/1‑94 (MQ=60)
                                                                      GAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCA                        <  SRR4302533.5841229/94‑1 (MQ=60)
                                                                       AACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAA                       >  SRR4302533.358713/1‑94 (MQ=60)
                                                                        ACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAA                      >  SRR4302533.757885/1‑94 (MQ=60)
                                                                        ACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAA                      >  SRR4302533.3216434/1‑94 (MQ=60)
                                                                        ACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAA                      >  SRR4302533.2574966/1‑94 (MQ=60)
                                                                         CTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAA                     >  SRR4302533.5284686/1‑94 (MQ=60)
                                                                           TTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAG                   <  SRR4302533.3099147/94‑1 (MQ=60)
                                                                             GCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGTG                 <  SRR4302533.6203176/94‑1 (MQ=60)
                                                                             GCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGTG                 <  SRR4302533.2259764/94‑1 (MQ=60)
                                                                              CGNANCNGNAANANCGGAGNAACCGGGTAANCAAAATCTACCNGGTGCCGAAGAGGNCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGTGA                <  SRR4302533.929830/94‑1 (MQ=60)
                                                                              CGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGTGA                >  SRR4302533.651271/1‑94 (MQ=60)
                                                                              CGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGTGA                >  SRR4302533.1577750/1‑94 (MQ=60)
                                                                               GAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGTGAT               >  SRR4302533.5587260/1‑94 (MQ=60)
                                                                               GAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGTGAT               <  SRR4302533.2397238/94‑1 (MQ=60)
                                                                               GAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGTGAT               >  SRR4302533.549242/1‑94 (MQ=60)
                                                                                AAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGTGATG              >  SRR4302533.946640/1‑94 (MQ=60)
                                                                                    AGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGTGATGAGAT          >  SRR4302533.4042992/1‑94 (MQ=60)
                                                                                    AGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGTGATGAGAT          >  SRR4302533.2688057/1‑94 (MQ=60)
                                                                                     GGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGTGATGAGATA         <  SRR4302533.5580059/94‑1 (MQ=60)
                                                                                       AACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGTGATGAGATAAC       >  SRR4302533.2817308/1‑94 (MQ=60)
                                                                                       AACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGTGATGAGATAAC       <  SRR4302533.4199021/94‑1 (MQ=60)
                                                                                        ACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGTGATGAGATAACG      <  SRR4302533.68879/94‑1 (MQ=60)
                                                                                        ACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGTGATGAGATAACG      >  SRR4302533.4086242/1‑94 (MQ=60)
                                                                                         CACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGTGATGAGATAACGG     <  SRR4302533.2885916/94‑1 (MQ=60)
                                                                                         CACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGTGATGAGATAACGG     >  SRR4302533.2002388/1‑94 (MQ=60)
                                                                                          ACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGTGATGAGATAACGGG    <  SRR4302533.637147/94‑1 (MQ=60)
                                                                                           CCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGTGATGAGATAACGGGT   >  SRR4302533.5914308/1‑94 (MQ=60)
                                                                                            CGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGTGATGAGATAACGGGTC  <  SRR4302533.2780973/94‑1 (MQ=60)
                                                                                            CGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGTGATGAGATAACGGGTC  <  SRR4302533.4233480/94‑1 (MQ=60)
                                                                                            CGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGTGATGAGATAACGGGTC  >  SRR4302533.210318/1‑94 (MQ=60)
                                                                                             |                                                                                            
CACCTGTTCGGTGTTACCGGAAGAGAATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCAGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGTGATGAGATAACGGGTC  >  NC_000913/3433962‑3434147

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑