Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,484,054 T→C Y100H (TAC→CAC)  crp → cyclic AMP receptor protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,484,0540TC100.0% 836.0 / NA 259Y100H (TAC→CAC) crpcyclic AMP receptor protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (126/133);  total (126/133)

Only 100 of 264 total aligned reads displayed.

TTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCA  >  NC_000913/3483964‑3484143
                                                                                          |                                                                                         
tttATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCAc                                                                                         <  2:4158033/93‑1 (MQ=255)
   aTTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACaaa                                                                                      >  1:5720760/1‑93 (MQ=255)
   aTTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACaaa                                                                                      >  1:6176055/1‑93 (MQ=255)
   aTTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACaaa                                                                                      <  1:5179205/93‑1 (MQ=255)
    ttGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACaaaa                                                                                     >  1:1245745/1‑93 (MQ=255)
    ttGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACaaaa                                                                                     <  1:969768/93‑1 (MQ=255)
      ggCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACaaaaaa                                                                                   <  1:5064807/93‑1 (MQ=255)
      ggCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACaaaaaa                                                                                   <  2:1683233/93‑1 (MQ=255)
      ggCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACaaaaaa                                                                                   <  1:2165404/93‑1 (MQ=255)
      ggCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACaaaaaa                                                                                   <  1:1890908/93‑1 (MQ=255)
       gCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAAt                                                                                  >  2:6187751/1‑93 (MQ=255)
       gCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAAt                                                                                  <  1:317350/93‑1 (MQ=255)
        cGAACTGGGCGTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAAtt                                                                                 <  1:5509572/93‑1 (MQ=255)
        cGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAAtt                                                                                 <  1:1421046/93‑1 (MQ=255)
        cGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAAtt                                                                                 <  1:941238/93‑1 (MQ=255)
         gAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAAttt                                                                                >  1:1311222/1‑93 (MQ=255)
          aaCTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACAGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTc                                                                               >  2:1187238/1‑93 (MQ=255)
           aCTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCg                                                                              >  1:3399491/1‑93 (MQ=255)
              gggCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAACAATTTCGCCa                                                                           >  2:4067854/1‑93 (MQ=255)
               ggCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCaa                                                                          <  1:600740/93‑1 (MQ=255)
                gCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAAt                                                                         <  2:2092826/93‑1 (MQ=255)
                  cTGTTTGAAGAGTGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGGAATTTCGCACAAAAAATTTCGCCAATTg                                                                       >  2:1370331/1‑93 (MQ=255)
                  cTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTg                                                                       >  1:5952191/1‑93 (MQ=255)
                  cTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTg                                                                       >  2:659151/1‑93 (MQ=255)
                     tttGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGAtt                                                                    <  2:866975/93‑1 (MQ=255)
                      ttGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTc                                                                   <  1:5821920/93‑1 (MQ=255)
                        gAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAg                                                                 >  2:759399/1‑93 (MQ=255)
                        gAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAg                                                                 <  2:4463005/93‑1 (MQ=255)
                        gAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAg                                                                 <  2:2020392/93‑1 (MQ=255)
                         aaGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAgg                                                                >  1:3971937/1‑93 (MQ=255)
                         aaGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAgg                                                                <  2:3785837/93‑1 (MQ=255)
                          agagGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGt                                                               >  1:1009595/1‑93 (MQ=255)
                          agagGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGt                                                               >  1:4648008/1‑93 (MQ=255)
                             gggCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTaaa                                                            <  2:2960813/93‑1 (MQ=255)
                              ggCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAAc                                                           <  2:2213891/93‑1 (MQ=255)
                                ccAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAAccc                                                         >  1:4557464/1‑93 (MQ=255)
                                ccAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAAccc                                                         <  1:753892/93‑1 (MQ=255)
                                 cacgAACGTAGCCCATGGGTACGGCCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCg                                                        <  2:1280742/90‑1 (MQ=255)
                                  agcaaCGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCgg                                                       <  2:3642010/90‑1 (MQ=255)
                                  aGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCgg                                                       >  2:2533273/1‑93 (MQ=255)
                                   ggAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGa                                                      >  2:1767625/1‑93 (MQ=255)
                                   ggAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGATAAACCCGGa                                                      >  2:2007236/1‑93 (MQ=255)
                                    gAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGAc                                                     >  1:5519171/1‑93 (MQ=255)
                                       cGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACAtt                                                  >  1:3871690/1‑93 (MQ=255)
                                        gTAGCGCATGGGTACGTGCGAAACCCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTc                                                 <  2:1867009/93‑1 (MQ=255)
                                        gTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTc                                                 <  2:3014310/93‑1 (MQ=255)
                                        gTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTc                                                 <  1:1774861/93‑1 (MQ=255)
                                        gTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTc                                                 <  1:2415580/93‑1 (MQ=255)
                                         tAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCt                                                >  2:4857891/1‑93 (MQ=255)
                                           gcgcATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGa                                              <  2:3607608/93‑1 (MQ=255)
                                           gcgcATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAAACCGGACATTCTGa                                              >  1:3020409/1‑93 (MQ=255)
                                            cgcATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCTGGTAAACCCGGACATTCTGAt                                             <  2:6296494/93‑1 (MQ=255)
                                            cgcATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGAt                                             <  2:2703157/93‑1 (MQ=255)
                                             gcATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATg                                            >  2:3266127/1‑93 (MQ=255)
                                              cATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGc                                           >  1:1161020/1‑93 (MQ=255)
                                                 gggTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGtt                                        <  2:5791540/93‑1 (MQ=255)
                                                  ggTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGttt                                       >  1:4184902/1‑93 (MQ=255)
                                                  ggTACGTGCGAAAACCGCCGGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGttt                                       >  2:6082629/1‑93 (MQ=255)
                                                           gAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGcaca                              >  2:5506688/1‑93 (MQ=255)
                                                           gAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGcaca                              <  1:2532010/93‑1 (MQ=255)
                                                           gAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTATGATGCGTTTGTCTGcaca                              >  1:4967223/1‑93 (MQ=255)
                                                            aaaaCCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAg                             >  2:6047556/1‑93 (MQ=255)
                                                             aaaCCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGa                            >  1:5787744/1‑93 (MQ=255)
                                                             aaaCCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGa                            <  1:3103941/93‑1 (MQ=255)
                                                             aaaCCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGa                            <  1:251432/93‑1 (MQ=255)
                                                              aaCCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGAt                           <  1:2228803/93‑1 (MQ=255)
                                                              aaCCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGAt                           <  2:4783272/93‑1 (MQ=255)
                                                              aaCCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGAt                           >  1:867261/1‑93 (MQ=255)
                                                               aCCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATg                          >  2:4629989/1‑93 (MQ=255)
                                                               aCCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATg                          >  1:301662/1‑93 (MQ=255)
                                                                ccGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCTAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATgg                         <  2:1229723/93‑1 (MQ=255)
                                                                ccGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATgg                         <  1:4681011/93‑1 (MQ=255)
                                                                 cGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGgc                        >  2:3861677/1‑93 (MQ=255)
                                                                 cGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGgc                        <  1:3741766/93‑1 (MQ=255)
                                                                 cGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGgc                        <  2:4887714/93‑1 (MQ=255)
                                                                 cGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGgc                        <  2:1826125/93‑1 (MQ=255)
                                                                   ccTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGgcgc                      <  1:5302852/93‑1 (MQ=255)
                                                                    cTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGcgcg                     >  1:663674/1‑93 (MQ=255)
                                                                     tgtgAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGcgt                    >  1:1692471/1‑93 (MQ=255)
                                                                     tgtgAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGcgt                    <  1:6233632/93‑1 (MQ=255)
                                                                      gtgAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGcgtc                   >  2:2277320/1‑93 (MQ=255)
                                                                      gtgAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGcgtc                   <  2:4988421/93‑1 (MQ=255)
                                                                        gAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGcgtcgt                 <  2:745363/93‑1 (MQ=255)
                                                                           gTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTg              >  2:3915944/1‑93 (MQ=255)
                                                                           gTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTg              >  1:3332979/1‑93 (MQ=255)
                                                                            tGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGc             <  1:3783767/93‑1 (MQ=255)
                                                                            tGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGc             >  2:2426121/1‑93 (MQ=255)
                                                                             ggCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCa            >  2:626142/1‑93 (MQ=255)
                                                                             ggCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCa            >  2:1587750/1‑93 (MQ=255)
                                                                             ggCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCa            >  1:6049177/1‑93 (MQ=255)
                                                                              gCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCaa           <  1:4479247/93‑1 (MQ=255)
                                                                              gCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCaa           >  2:4110340/1‑93 (MQ=255)
                                                                               cTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAg          <  2:4478621/93‑1 (MQ=255)
                                                                               cTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAg          >  1:200881/1‑93 (MQ=255)
                                                                               cTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAg          >  1:2918761/1‑93 (MQ=255)
                                                                               cTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAg          <  1:2332077/93‑1 (MQ=255)
                                                                                 gAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTc        >  2:5234480/1‑93 (MQ=255)
                                                                                    aTTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACt     <  2:5462071/93‑1 (MQ=255)
                                                                                      ttCGCACAAAAAATTTCGCCGATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTc   <  1:4207019/93‑1 (MQ=255)
                                                                                       tCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCa  <  1:789776/93‑1 (MQ=255)
                                                                                          |                                                                                         
TTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCA  >  NC_000913/3483964‑3484143

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGA  >  NC_000913/3483961‑3484147
                                                                                             |                                                                                             
GATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGTCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGC                                                                                               <  SRR4302533.3809446/94‑1 (MQ=60)
GATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGC                                                                                               <  SRR4302533.6064214/94‑1 (MQ=60)
GATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGC                                                                                               <  SRR4302533.5214050/94‑1 (MQ=60)
     TATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAA                                                                                          >  SRR4302533.6213163/1‑94 (MQ=60)
     TATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAA                                                                                          >  SRR4302533.5755300/1‑94 (MQ=60)
      ATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAA                                                                                         >  SRR4302533.1253182/1‑94 (MQ=60)
      ATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAA                                                                                         >  SRR4302533.220663/1‑94 (MQ=60)
      ATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAA                                                                                         <  SRR4302533.5210483/94‑1 (MQ=60)
       TTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAA                                                                                        <  SRR4302533.975458/94‑1 (MQ=60)
        TGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAA                                                                                       <  SRR4302533.1344957/94‑1 (MQ=60)
         GGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAAT                                                                                      <  SRR4302533.2178724/94‑1 (MQ=60)
         GGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAAT                                                                                      <  SRR4302533.5095401/94‑1 (MQ=60)
         GGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAAT                                                                                      <  SRR4302533.1902452/94‑1 (MQ=60)
          GCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATT                                                                                     <  SRR4302533.319293/94‑1 (MQ=60)
           CGAACTGGGCGTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTT                                                                                    <  SRR4302533.5542831/94‑1 (MQ=60)
           CGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTT                                                                                    <  SRR4302533.1429642/94‑1 (MQ=60)
           CGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTT                                                                                    >  SRR4302533.1319094/1‑94 (MQ=60)
           CGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTT                                                                                    >  SRR4302533.2553773/1‑94 (MQ=60)
           CGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTT                                                                                    <  SRR4302533.946774/94‑1 (MQ=60)
            GAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGAGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTC                                                                                   >  SRR4302533.6071428/1‑94 (MQ=60)
             AACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCG                                                                                  >  SRR4302533.3420482/1‑94 (MQ=60)
             AACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCG                                                                                  <  SRR4302533.463583/94‑1 (MQ=60)
              ACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGC                                                                                 >  SRR4302533.1828791/1‑94 (MQ=60)
                 GGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAA                                                                              <  SRR4302533.918970/94‑1 (MQ=60)
                  GGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAAT                                                                             <  SRR4302533.604271/94‑1 (MQ=60)
                   GCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATT                                                                            >  SRR4302533.5095812/1‑94 (MQ=60)
                    CCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTG                                                                           >  SRR4302533.5988195/1‑94 (MQ=60)
                    CCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTG                                                                           <  SRR4302533.3092344/94‑1 (MQ=60)
                    CCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTG                                                                           >  SRR4302533.1221841/1‑94 (MQ=60)
                     CTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGA                                                                          >  SRR4302533.286056/1‑94 (MQ=60)
                     CTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGA                                                                          >  SRR4302533.4682785/1‑94 (MQ=60)
                      TGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGAT                                                                         >  SRR4302533.5054304/1‑94 (MQ=60)
                      TGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGCGGCTGAAATTTCGCACAAAAAATTTCCCCAATCGAT                                                                         >  SRR4302533.4212992/1‑94 (MQ=60)
                        TTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTC                                                                       >  SRR4302533.831734/1‑94 (MQ=60)
                         TTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCA                                                                      <  SRR4302533.5857087/94‑1 (MQ=60)
                         TTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCA                                                                      <  SRR4302533.5623323/94‑1 (MQ=60)
                         TTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCA                                                                      <  SRR4302533.5823788/94‑1 (MQ=60)
                          TGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAG                                                                     <  SRR4302533.639935/94‑1 (MQ=60)
                           GAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGG                                                                    <  SRR4302533.6171606/94‑1 (MQ=60)
                           GAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGG                                                                    >  SRR4302533.3996127/1‑94 (MQ=60)
                           GAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGG                                                                    <  SRR4302533.2949681/94‑1 (MQ=60)
                            AAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGT                                                                   >  SRR4302533.1015500/1‑94 (MQ=60)
                            AAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGT                                                                   <  SRR4302533.5551874/94‑1 (MQ=60)
                            AAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGT                                                                   >  SRR4302533.4676172/1‑94 (MQ=60)
                             AGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTA                                                                  <  SRR4302533.3306974/94‑1 (MQ=60)
                              GAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAA                                                                 <  SRR4302533.4620264/94‑1 (MQ=60)
                               AGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAA                                                                >  SRR4302533.5740233/1‑94 (MQ=60)
                               AGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAA                                                                <  SRR4302533.793964/94‑1 (MQ=60)
                                 GGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACC                                                              >  SRR4302533.5073434/1‑94 (MQ=60)
                                 GGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACC                                                              <  SRR4302533.1730885/94‑1 (MQ=60)
                                  GCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCC                                                             >  SRR4302533.4585102/1‑94 (MQ=60)
                                   CCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCG                                                            >  SRR4302533.1998814/1‑94 (MQ=60)
                                   CCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCG                                                            <  SRR4302533.957051/94‑1 (MQ=60)
                                   CCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCG                                                            <  SRR4302533.758331/94‑1 (MQ=60)
                                   CCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCG                                                            <  SRR4302533.231589/94‑1 (MQ=60)
                                     AGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGA                                                          >  SRR4302533.1301954/1‑94 (MQ=60)
                                     AGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGA                                                          >  SRR4302533.5942455/1‑94 (MQ=60)
                                     AGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGA                                                          >  SRR4302533.6068552/1‑94 (MQ=60)
                                      GGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGAC                                                         >  SRR4302533.5552495/1‑94 (MQ=60)
                                        AACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACAT                                                       >  SRR4302533.3918269/1‑94 (MQ=60)
                                         ACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATT                                                      >  SRR4302533.3895321/1‑94 (MQ=60)
                                          CGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTC                                                     <  SRR4302533.3163884/94‑1 (MQ=60)
                                          CGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTC                                                     <  SRR4302533.3581976/94‑1 (MQ=60)
                                           GTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCT                                                    <  SRR4302533.2430503/94‑1 (MQ=60)
                                           GTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCT                                                    <  SRR4302533.1785671/94‑1 (MQ=60)
                                           GTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCT                                                    <  SRR4302533.3750735/94‑1 (MQ=60)
                                            TAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTG                                                   >  SRR4302533.4567372/1‑94 (MQ=60)
                                             AGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGA                                                  >  SRR4302533.297839/1‑94 (MQ=60)
                                             AGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAAACCGGACATTCTGA                                                  >  SRR4302533.3038896/1‑94 (MQ=60)
                                              GCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGAT                                                 <  SRR4302533.2249785/94‑1 (MQ=60)
                                              GCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGAT                                                 <  SRR4302533.2359354/94‑1 (MQ=60)
                                               CGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATG                                                >  SRR4302533.2476722/1‑94 (MQ=60)
                                               CGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATG                                                >  SRR4302533.676986/1‑94 (MQ=60)
                                                GCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGC                                               >  SRR4302533.1167987/1‑94 (MQ=60)
                                                GCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGC                                               >  SRR4302533.2021789/1‑94 (MQ=60)
                                                GCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGC                                               >  SRR4302533.3126126/1‑94 (MQ=60)
                                                GCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGC                                               >  SRR4302533.1991221/1‑94 (MQ=60)
                                                 CATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCG                                              <  SRR4302533.5931804/94‑1 (MQ=60)
                                                 CATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCG                                              >  SRR4302533.833824/1‑94 (MQ=60)
                                                  ATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGT                                             >  SRR4302533.3062615/1‑94 (MQ=60)
                                                   TGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTT                                            <  SRR4302533.1519487/94‑1 (MQ=60)
                                                   TGGGTACGTGCGAAAACCGCCTATGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTT                                            >  SRR4302533.377496/1‑94 (MQ=60)
                                                    GGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTT                                           >  SRR4302533.5287529/1‑94 (MQ=60)
                                                    GGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTT                                           >  SRR4302533.4210241/1‑94 (MQ=60)
                                                     GGTACTTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTG                                          <  SRR4302533.1888621/94‑1 (MQ=60)
                                                      GTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGT                                         <  SRR4302533.6014180/94‑1 (MQ=60)
                                                        ACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCT                                       <  SRR4302533.1066767/94‑1 (MQ=60)
                                                         CGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTG                                      <  SRR4302533.5216638/94‑1 (MQ=60)
                                                         CGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTG                                      >  SRR4302533.4077542/1‑94 (MQ=60)
                                                          GTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGC                                     >  SRR4302533.2199989/1‑94 (MQ=60)
                                                          GTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGC                                     <  SRR4302533.5815756/94‑1 (MQ=60)
                                                             CGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACA                                  <  SRR4302533.2453186/94‑1 (MQ=60)
                                                             CGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTATGATGCGTTTGTCTGCACA                                  >  SRR4302533.4997339/1‑94 (MQ=60)
                                                              GAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAG                                 <  SRR4302533.2547598/94‑1 (MQ=60)
                                                              GAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAG                                 <  SRR4302533.3383701/94‑1 (MQ=60)
                                                               AAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGA                                >  SRR4302533.695351/1‑94 (MQ=60)
                                                               AAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGA                                >  SRR4302533.6263900/1‑94 (MQ=60)
                                                               AAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGA                                >  SRR4302533.5822711/1‑94 (MQ=60)
                                                                AAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGAT                               >  SRR4302533.3918483/1‑94 (MQ=60)
                                                                AAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGAT                               <  SRR4302533.3123024/94‑1 (MQ=60)
                                                                AAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGAT                               <  SRR4302533.252981/94‑1 (MQ=60)
                                                                AAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGAT                               >  SRR4302533.872344/1‑94 (MQ=60)
                                                                AAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGAT                               >  SRR4302533.5361438/1‑94 (MQ=60)
                                                                 AACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATG                              <  SRR4302533.2242559/94‑1 (MQ=60)
                                                                 AACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATG                              >  SRR4302533.303505/1‑94 (MQ=60)
                                                                  ACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGG                             <  SRR4302533.4929259/94‑1 (MQ=60)
                                                                   CCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGC                            <  SRR4302533.443934/94‑1 (MQ=60)
                                                                   CCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGC                            <  SRR4302533.4709375/94‑1 (MQ=60)
                                                                    CGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCG                           <  SRR4302533.3764714/94‑1 (MQ=60)
                                                                     GCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGC                          >  SRR4302533.149698/1‑94 (MQ=60)
                                                                      CCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCG                         >  SRR4302533.667592/1‑94 (MQ=60)
                                                                      CCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCG                         <  SRR4302533.5334621/94‑1 (MQ=60)
                                                                       CTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGT                        >  SRR4302533.4228551/1‑94 (MQ=60)
                                                                       CTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGT                        >  SRR4302533.1702724/1‑94 (MQ=60)
                                                                        TGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTC                       >  SRR4302533.3811497/1‑94 (MQ=60)
                                                                        TGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTC                       <  SRR4302533.6270955/94‑1 (MQ=60)
                                                                          TGAAGTGGCTGAAATTTCGCACAAGAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGTACAGATGGCGCGTCGT                     >  SRR4302533.5827279/1‑94 (MQ=60)
                                                                          TGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGT                     >  SRR4302533.1325614/1‑94 (MQ=60)
                                                                           GAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCTGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTC                    >  SRR4302533.3036402/1‑94 (MQ=60)
                                                                             AGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTG                  >  SRR4302533.3353573/1‑94 (MQ=60)
                                                                             AGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTG                  <  SRR4302533.3877997/94‑1 (MQ=60)
                                                                               TGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCA                >  SRR4302533.6085826/1‑94 (MQ=60)
                                                                               TGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCA                <  SRR4302533.3806936/94‑1 (MQ=60)
                                                                                GGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAA               >  SRR4302533.3416946/1‑94 (MQ=60)
                                                                                 GCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAG              <  SRR4302533.4506345/94‑1 (MQ=60)
                                                                                 GCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAG              >  SRR4302533.202155/1‑94 (MQ=60)
                                                                                 GCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAG              >  SRR4302533.897099/1‑94 (MQ=60)
                                                                                 GCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAG              >  SRR4302533.2936647/1‑94 (MQ=60)
                                                                                  CTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGT             <  SRR4302533.2346516/94‑1 (MQ=60)
                                                                                  CTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGT             <  SRR4302533.6305846/94‑1 (MQ=60)
                                                                                      AATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACT         <  SRR4302533.6139518/94‑1 (MQ=60)
                                                                                       ATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTT        >  SRR4302533.2542788/1‑94 (MQ=60)
                                                                                        TTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTC       <  SRR4302533.5498457/94‑1 (MQ=60)
                                                                                         TTCGCACAAAAAATTTCGCCGATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCA      <  SRR4302533.4232469/94‑1 (MQ=60)
                                                                                         TTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCA      >  SRR4302533.4804591/1‑94 (MQ=60)
                                                                                          TCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAG     <  SRR4302533.4551920/94‑1 (MQ=60)
                                                                                          TCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAG     <  SRR4302533.794425/94‑1 (MQ=60)
                                                                                          TCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAG     <  SRR4302533.1219535/94‑1 (MQ=60)
                                                                                           CGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGA    <  SRR4302533.4937638/94‑1 (MQ=60)
                                                                                           CGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGA    >  SRR4302533.4888716/1‑94 (MQ=60)
                                                                                            GCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAG   <  SRR4302533.2321454/94‑1 (MQ=60)
                                                                                            GCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAG   >  SRR4302533.4114985/1‑94 (MQ=60)
                                                                                             CACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGA  >  SRR4302533.4860388/1‑94 (MQ=60)
                                                                                             |                                                                                             
GATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGA  >  NC_000913/3483961‑3484147

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑