Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,484,151 T→C V132A (GTG→GCG)  crp → cyclic AMP receptor protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,484,1510TC99.4% 1505.2 / ‑2.9 467V132A (GTG→GCG) crpcyclic AMP receptor protein
Reads supporting (aligned to +/- strand):  ref base T (2/1);  new base C (202/262);  total (204/263)
Fisher's exact test for biased strand distribution p-value = 5.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.11e-01

Only 100 of 476 total aligned reads displayed.

AATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGG  >  NC_000913/3484061‑3484240
                                                                                          |                                                                                         
aaTTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCgg                                                                                         >  1:2874318/1‑93 (MQ=255)
 aTTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCggg                                                                                        <  1:7835140/93‑1 (MQ=255)
 aTTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCggg                                                                                        >  2:3206309/1‑93 (MQ=255)
  tttCGCGAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGc                                                                                       <  1:8935876/93‑1 (MQ=255)
  tttCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGc                                                                                       <  1:1413467/93‑1 (MQ=255)
  tttCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGc                                                                                       >  1:8295281/1‑93 (MQ=255)
     cGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAAc                                                                                    <  1:1072088/93‑1 (MQ=255)
     cGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAAc                                                                                    <  2:277214/93‑1 (MQ=255)
      gCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAAcc                                                                                   >  1:3839318/1‑93 (MQ=255)
        cAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTg                                                                                 <  1:6235369/93‑1 (MQ=255)
        cAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTg                                                                                 <  2:6796296/93‑1 (MQ=255)
        cAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCACGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTg                                                                                 <  1:428210/93‑1 (MQ=255)
         aaTTGATTCAGGTAACCCCGGTCATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTgg                                                                                <  2:5153561/93‑1 (MQ=255)
         aaTTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTgg                                                                                >  1:9632215/1‑93 (MQ=255)
           ttGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCg                                                                              <  1:4753656/93‑1 (MQ=255)
             gATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGtt                                                                            >  2:696463/1‑93 (MQ=255)
              aTTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTc                                                                           <  1:3657186/93‑1 (MQ=255)
              aTTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTc                                                                           <  2:9715501/93‑1 (MQ=255)
               ttCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTcc                                                                          >  2:4895791/1‑93 (MQ=255)
               ttCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTcc                                                                          >  1:9107722/1‑93 (MQ=255)
               ttCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTcc                                                                          >  1:3263208/1‑93 (MQ=255)
                 cAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTc                                                                        <  1:714512/93‑1 (MQ=255)
                  aGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCg                                                                       <  1:2148569/93‑1 (MQ=255)
                   ggTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGa                                                                      <  2:1474235/93‑1 (MQ=255)
                   ggTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGa                                                                      >  1:1212861/1‑93 (MQ=255)
                   ggTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGa                                                                      <  1:3127871/93‑1 (MQ=255)
                   ggTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGa                                                                      >  2:9379280/1‑93 (MQ=255)
                   ggTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGa                                                                      >  2:8480576/1‑93 (MQ=255)
                   ggTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGa                                                                      <  2:8296135/93‑1 (MQ=255)
                   agTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGa                                                                      <  2:9326394/92‑1 (MQ=255)
                    gTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGAc                                                                     <  1:6549106/93‑1 (MQ=255)
                      aaaCCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGt                                                                   >  2:2237550/1‑93 (MQ=255)
                       aaCCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTg                                                                  <  1:7399625/93‑1 (MQ=255)
                         cccGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGAc                                                                <  2:5769827/93‑1 (MQ=255)
                          ccGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACg                                                               >  2:4564745/1‑93 (MQ=255)
                           cGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCgacgggacgg                                                              <  2:6473625/93‑1 (MQ=255)
                             gACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGc                                                            <  2:8137238/93‑1 (MQ=255)
                             gACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGc                                                            <  1:9815739/93‑1 (MQ=255)
                             gACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGc                                                            >  1:3585979/1‑93 (MQ=255)
                             gACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGc                                                            >  1:5656395/1‑93 (MQ=255)
                             gACAATATGATGAGTATATATGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGAAGTGACgaga                                                            <  2:8969932/93‑4 (MQ=255)
                              aCATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGcc                                                           >  1:7300739/1‑93 (MQ=255)
                               cgttCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCg                                                          >  1:1719174/3‑93 (MQ=255)
                                aTTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGc                                                         <  2:7168327/93‑1 (MQ=255)
                                 ttCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCa                                                        <  2:4817943/93‑1 (MQ=255)
                                 ttCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCa                                                        <  1:5400482/93‑1 (MQ=255)
                                 ttCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCa                                                        >  2:4160649/1‑93 (MQ=255)
                                  tCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCAt                                                       <  1:8780551/93‑1 (MQ=255)
                                  tCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCAt                                                       <  2:7565605/93‑1 (MQ=255)
                                   cTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCACGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCAtt                                                      >  2:1612094/1‑93 (MQ=255)
                                    tGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTg                                                     >  2:6442756/1‑93 (MQ=255)
                                    tGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTg                                                     <  2:8763725/93‑1 (MQ=255)
                                     gATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGc                                                    >  2:7435958/1‑93 (MQ=255)
                                     gATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGc                                                    <  2:5908853/93‑1 (MQ=255)
                                      aTGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGca                                                   <  2:3830706/93‑1 (MQ=255)
                                       tGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGcac                                                  <  1:4910395/93‑1 (MQ=255)
                                       tGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGcac                                                  <  2:2526654/93‑1 (MQ=255)
                                        gCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGcaca                                                 <  1:2055827/93‑1 (MQ=255)
                                           tttGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGAc                                              >  1:8484416/1‑93 (MQ=255)
                                           tttGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGAc                                              >  1:5571774/1‑93 (MQ=255)
                                           tttGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGAc                                              <  1:6439244/93‑1 (MQ=255)
                                            ttGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGAct                                             >  2:3634035/1‑93 (MQ=255)
                                            ttGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGAct                                             >  1:5902272/1‑93 (MQ=255)
                                            ttGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGAct                                             <  1:671477/93‑1 (MQ=255)
                                               tCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTctg                                          <  1:4204371/93‑1 (MQ=255)
                                                 tGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTctgct                                        >  2:9013873/1‑93 (MQ=255)
                                                 tGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTctgct                                        <  2:3023847/93‑1 (MQ=255)
                                                 tGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTctgct                                        >  2:1255436/1‑93 (MQ=255)
                                                   cacaGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGa                                      >  1:545714/1‑93 (MQ=255)
                                                     caGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAAt                                    <  1:7707681/93‑1 (MQ=255)
                                                     caGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAAt                                    <  2:7926570/93‑1 (MQ=255)
                                                      aGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATc                                   <  1:9273002/93‑1 (MQ=255)
                                                        aTGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTg                                 <  1:444132/93‑1 (MQ=255)
                                                            cgcgTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCaa                             <  1:4604401/93‑1 (MQ=255)
                                                             gcgTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCaaa                            >  1:6931823/1‑93 (MQ=255)
                                                              cgtcgtCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCaaaa                           >  2:5542933/1‑93 (MQ=255)
                                                               gtcgtcTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGAAGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAaac                          <  1:8634056/93‑1 (MQ=255)
                                                                  gtcTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCACaacaac                       >  2:1917265/1‑93 (MQ=255)
                                                                  gtcTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAaacaac                       <  1:2011867/93‑1 (MQ=255)
                                                                  gtcTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAaacaac                       <  1:10038616/93‑1 (MQ=255)
                                                                   tcTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAAcc                      >  1:5004331/1‑93 (MQ=255)
                                                                     tGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAg                    >  2:2866052/1‑93 (MQ=255)
                                                                       cAAGTCACTTCAGAGAAAGCGGGCAACCTCGCGTTCCTCGACGTGAGGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGAc                  <  1:3071894/93‑1 (MQ=255)
                                                                         aGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGc                >  2:3915207/1‑93 (MQ=255)
                                                                         aGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGc                >  1:8195374/1‑93 (MQ=255)
                                                                          gtaaCTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCt               <  1:2677415/90‑1 (MQ=255)
                                                                          gTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCt               <  2:953706/93‑1 (MQ=255)
                                                                          gTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCt               <  1:8032820/93‑1 (MQ=255)
                                                                           tCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTa              >  1:3956483/1‑93 (MQ=255)
                                                                           tCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTa              <  2:5726099/93‑1 (MQ=255)
                                                                           tCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTa              >  1:2290374/1‑93 (MQ=255)
                                                                            cACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTAt             <  1:1318605/93‑1 (MQ=255)
                                                                             aCTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATg            >  2:6144510/1‑93 (MQ=255)
                                                                                tCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACt         >  1:7589796/1‑93 (MQ=255)
                                                                                  agagAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCa       <  2:9870554/93‑1 (MQ=255)
                                                                                   gagaAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAc      >  2:5370071/1‑93 (MQ=255)
                                                                                    agaAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAcc     <  2:6569317/93‑1 (MQ=255)
                                                                                     gaAAGCGGGCAACCTGGCGCTCCGCGACGTGACGGGTCGCATTTCACAGCCTCTGCTGAATCTGGCAAAACAACCAGACGCTATGGCTCAccc    <  2:1120216/93‑1 (MQ=255)
                                                                                       aaGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCgg  >  2:8652315/1‑93 (MQ=255)
                                                                                       aaGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCgg  <  1:2498743/93‑1 (MQ=255)
                                                                                          |                                                                                         
AATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGG  >  NC_000913/3484061‑3484240

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 271 total aligned reads displayed.
AAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGG  >  NC_000913/3484059‑3484244
                                                                                            |                                                                                             
AAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGCAAGCG                                                                                              >  SRR4302540.5006333/1‑94 (MQ=60)
AAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCG                                                                                              <  SRR4302540.8308997/94‑1 (MQ=60)
 AAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGG                                                                                             <  SRR4302540.647378/94‑1 (MQ=60)
 AAAATTCGACAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGG                                                                                             <  SRR4302540.1070979/94‑1 (MQ=60)
  AATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGG                                                                                            >  SRR4302540.6763784/1‑94 (MQ=60)
    TTTCGCGAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCA                                                                                          <  SRR4302540.9040451/94‑1 (MQ=60)
    TTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCA                                                                                          <  SRR4302540.1428715/94‑1 (MQ=60)
     TTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAA                                                                                         <  SRR4302540.5482691/94‑1 (MQ=60)
     TTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAA                                                                                         <  SRR4302540.6559041/94‑1 (MQ=60)
      TCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAAC                                                                                        <  SRR4302540.2602140/94‑1 (MQ=60)
      TCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAAC                                                                                        <  SRR4302540.7010131/94‑1 (MQ=60)
      TCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTATGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAAC                                                                                        <  SRR4302540.2557126/94‑1 (MQ=60)
       CGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACC                                                                                       >  SRR4302540.3881070/1‑94 (MQ=60)
       CGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGACACTTCAGAGAAAGCGGGCAACC                                                                                       >  SRR4302540.3509737/1‑94 (MQ=60)
         CCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTG                                                                                     <  SRR4302540.5052237/94‑1 (MQ=60)
          CAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGG                                                                                    >  SRR4302540.9745713/1‑94 (MQ=60)
          CAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGG                                                                                    <  SRR4302540.6304719/94‑1 (MQ=60)
          CAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGG                                                                                    <  SRR4302540.4252736/94‑1 (MQ=60)
          CAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCACGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGG                                                                                    <  SRR4302540.432713/94‑1 (MQ=60)
           AATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGC                                                                                   >  SRR4302540.8165303/1‑94 (MQ=60)
           AATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGC                                                                                   <  SRR4302540.4260847/94‑1 (MQ=60)
           AATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGC                                                                                   >  SRR4302540.2120444/1‑94 (MQ=60)
            ATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCG                                                                                  >  SRR4302540.1022555/1‑94 (MQ=60)
            ATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCG                                                                                  >  SRR4302540.1031823/1‑94 (MQ=60)
              TGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTT                                                                                <  SRR4302540.6997032/94‑1 (MQ=60)
                ATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCC                                                                              >  SRR4302540.3298816/1‑94 (MQ=60)
                ATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCC                                                                              >  SRR4302540.9217178/1‑94 (MQ=60)
                 TTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCT                                                                             >  SRR4302540.1148554/1‑94 (MQ=60)
                 TTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCCGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCT                                                                             <  SRR4302540.8679690/94‑1 (MQ=60)
                 TTCAGGTAAACCCGGACATGCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCT                                                                             <  SRR4302540.1339041/94‑1 (MQ=60)
                   CAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCG                                                                           <  SRR4302540.722184/94‑1 (MQ=60)
                   CAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCG                                                                           <  SRR4302540.4557018/94‑1 (MQ=60)
                   CAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACATGGCGTTCCTCA                                                                           >  SRR4302540.5072263/1‑94 (MQ=60)
                    AGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGA                                                                          <  SRR4302540.2171672/94‑1 (MQ=60)
                    AGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGA                                                                          >  SRR4302540.1225294/1‑94 (MQ=60)
                    AGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGA                                                                          >  SRR4302540.2047801/1‑94 (MQ=60)
                    AGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGA                                                                          >  SRR4302540.4618758/1‑94 (MQ=60)
                     GGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGAC                                                                         <  SRR4302540.9251562/94‑1 (MQ=60)
                     GGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGAC                                                                         <  SRR4302540.3162018/94‑1 (MQ=60)
                     GGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGAC                                                                         >  SRR4302540.5836677/1‑94 (MQ=60)
                     GGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCGGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGAC                                                                         <  SRR4302540.3788362/94‑1 (MQ=60)
                      GTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACG                                                                        <  SRR4302540.6621748/94‑1 (MQ=60)
                      GTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACG                                                                        <  SRR4302540.7934327/94‑1 (MQ=60)
                       TAGACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGTGAAAGCGGGCAACCTGGCGTTCCTCGACGT                                                                       <  SRR4302540.9071398/94‑1 (MQ=60)
                       TAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGT                                                                       >  SRR4302540.4880199/1‑94 (MQ=60)
                       GAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGT                                                                       <  SRR4302540.3209147/94‑1 (MQ=60)
                        AAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTG                                                                      >  SRR4302540.516918/1‑94 (MQ=60)
                        AAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTG                                                                      <  SRR4302540.9026577/94‑1 (MQ=60)
                        AAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTG                                                                      >  SRR4302540.1002875/1‑94 (MQ=60)
                         AACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGA                                                                     <  SRR4302540.7482062/94‑1 (MQ=60)
                          gccgcgtgattcggattgGTTTGACTGCATAGATGGCGCGTTGTCGCCAAGTCCCTTGAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGAC                                                                    <  SRR4302540.1340591/76‑1 (MQ=31)
                          accccgtcattcgggtgcgtttgtctgcaactagGGCGCGTCGTCTGCAAGTCCCTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGAC                                                                    <  SRR4302540.485875/60‑1 (MQ=60)
                          ACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGAC                                                                    >  SRR4302540.1599304/1‑94 (MQ=60)
                          ACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGAC                                                                    <  SRR4302540.7923392/94‑1 (MQ=60)
                          ACCCAGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGGCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGAC                                                                    <  SRR4302540.1340593/94‑1 (MQ=60)
                           ccccgccatcctgatgcggtggtctgccccgctggcccctcggctgccAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACG                                                                   <  SRR4302540.7113507/46‑1 (MQ=60)
                           CCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAGCCTGGCGTTCCTCGACGTGACG                                                                   >  SRR4302540.5660505/1‑94 (MQ=60)
                           CCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACG                                                                   <  SRR4302540.6532770/94‑1 (MQ=60)
                            CCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGG                                                                  <  SRR4302540.1259780/94‑1 (MQ=60)
                            CCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGG                                                                  >  SRR4302540.8749067/1‑94 (MQ=60)
                             CGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGG                                                                 >  SRR4302540.6933816/1‑94 (MQ=60)
                             CGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGG                                                                 >  SRR4302540.4403477/1‑94 (MQ=60)
                              GGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGC                                                                <  SRR4302540.2085759/94‑1 (MQ=60)
                              GGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGC                                                                >  SRR4302540.5718679/1‑94 (MQ=60)
                               GACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCCCTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCC                                                               <  SRR4302540.898572/94‑1 (MQ=60)
                               GACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCC                                                               >  SRR4302540.7382229/1‑94 (MQ=60)
                               GACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCC                                                               <  SRR4302540.7765842/94‑1 (MQ=60)
                               GACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCC                                                               >  SRR4302540.7382230/1‑94 (MQ=60)
                                ACGTTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCG                                                              >  SRR4302540.1737733/1‑94 (MQ=60)
                                ACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCG                                                              >  SRR4302540.1737734/1‑94 (MQ=60)
                                 CATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGC                                                             >  SRR4302540.9303890/1‑94 (MQ=60)
                                 CATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGC                                                             >  SRR4302540.1107803/1‑94 (MQ=60)
                                 CATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGC                                                             >  SRR4302540.4095732/1‑94 (MQ=60)
                                 CATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGC                                                             >  SRR4302540.7059180/1‑94 (MQ=60)
                                  ATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCA                                                            <  SRR4302540.9794339/94‑1 (MQ=60)
                                  ATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCA                                                            >  SRR4302540.2469737/1‑94 (MQ=60)
                                   TTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCAT                                                           <  SRR4302540.8759042/94‑1 (MQ=60)
                                   TTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCAT                                                           <  SRR4302540.5987480/94‑1 (MQ=60)
                                    TCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATT                                                          <  SRR4302540.392518/94‑1 (MQ=60)
                                    TCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATT                                                          <  SRR4302540.8882215/94‑1 (MQ=60)
                                     CTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTG                                                         <  SRR4302540.8305538/94‑1 (MQ=60)
                                     CTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTG                                                         <  SRR4302540.3288348/94‑1 (MQ=60)
                                      TGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGC                                                        >  SRR4302540.6347288/1‑94 (MQ=60)
                                      TGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGC                                                        <  SRR4302540.6221139/94‑1 (MQ=60)
                                        ATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCAC                                                      <  SRR4302540.9745418/94‑1 (MQ=60)
                                        ATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCAC                                                      <  SRR4302540.4110200/94‑1 (MQ=60)
                                         TGCGTTTGTCTGCACAGATTGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACA                                                     <  SRR4302540.9891834/94‑1 (MQ=60)
                                         TGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACA                                                     <  SRR4302540.7463936/94‑1 (MQ=60)
                                         TGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACA                                                     <  SRR4302540.4796332/94‑1 (MQ=60)
                                         TGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACA                                                     <  SRR4302540.4963268/94‑1 (MQ=60)
                                          GCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAG                                                    >  SRR4302540.4745424/1‑94 (MQ=60)
                                          GCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAG                                                    >  SRR4302540.7764625/1‑94 (MQ=60)
                                          GCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAG                                                    <  SRR4302540.6106365/94‑1 (MQ=60)
                                            GTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGAC                                                  <  SRR4302540.2276164/94‑1 (MQ=60)
                                            GTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGAC                                                  >  SRR4302540.8582982/1‑94 (MQ=60)
                                            GTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGAC                                                  >  SRR4302540.9168981/1‑94 (MQ=60)
                                            GTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGAC                                                  <  SRR4302540.3145136/94‑1 (MQ=60)
                                            GTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGAC                                                  >  SRR4302540.5633124/1‑94 (MQ=60)
                                             TTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACT                                                 >  SRR4302540.7861927/1‑94 (MQ=60)
                                             TTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACT                                                 <  SRR4302540.8174979/94‑1 (MQ=60)
                                             TTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACT                                                 >  SRR4302540.5967452/1‑94 (MQ=60)
                                             TTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACT                                                 <  SRR4302540.6510908/94‑1 (MQ=60)
                                             TTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACT                                                 <  SRR4302540.4381735/94‑1 (MQ=60)
                                             TTTGTCTGCACAGAGGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACT                                                 <  SRR4302540.3972909/94‑1 (MQ=60)
                                              TTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTC                                                <  SRR4302540.678675/94‑1 (MQ=60)
                                              TTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTC                                                <  SRR4302540.8045482/94‑1 (MQ=60)
                                               TGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCT                                               <  SRR4302540.3675134/94‑1 (MQ=60)
                                               TGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCT                                               <  SRR4302540.7746874/94‑1 (MQ=60)
                                                GTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTG                                              >  SRR4302540.8681244/1‑94 (MQ=60)
                                                 TCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGC                                             >  SRR4302540.8781595/1‑94 (MQ=60)
                                                  CTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCT                                            <  SRR4302540.3642234/94‑1 (MQ=60)
                                                  CTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCT                                            >  SRR4302540.7617580/1‑94 (MQ=60)
                                                   TGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTG                                           >  SRR4302540.186380/1‑94 (MQ=60)
                                                    GCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGA                                          >  SRR4302540.551653/1‑94 (MQ=60)
                                                     CACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAA                                         >  SRR4302540.5476355/1‑94 (MQ=60)
                                                     CACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAA                                         <  SRR4302540.3118274/94‑1 (MQ=60)
                                                      ACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAAT                                        >  SRR4302540.5519944/1‑94 (MQ=60)
                                                       CAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATC                                       >  SRR4302540.2048033/1‑94 (MQ=60)
                                                       CAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATC                                       <  SRR4302540.8210786/94‑1 (MQ=60)
                                                       CAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATC                                       <  SRR4302540.3405268/94‑1 (MQ=60)
                                                        AGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCT                                      <  SRR4302540.9383876/94‑1 (MQ=60)
                                                        AGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCT                                      <  SRR4302540.2787700/94‑1 (MQ=60)
                                                        AGAGGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCT                                      <  SRR4302540.853205/94‑1 (MQ=60)
                                                         GATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTG                                     <  SRR4302540.8020628/94‑1 (MQ=60)
                                                         GATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTG                                     >  SRR4302540.272899/1‑94 (MQ=60)
                                                          ATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGG                                    >  SRR4302540.743787/1‑94 (MQ=60)
                                                          ATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGG                                    <  SRR4302540.5665419/94‑1 (MQ=60)
                                                          ATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGG                                    <  SRR4302540.448862/94‑1 (MQ=60)
                                                           TGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGC                                   <  SRR4302540.8531924/94‑1 (MQ=60)
                                                            GGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGAGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCA                                  >  SRR4302540.7443921/1‑94 (MQ=60)
                                                              CGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAA                                <  SRR4302540.4653861/94‑1 (MQ=60)
                                                              CGCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAA                                <  SRR4302540.2932177/94‑1 (MQ=60)
                                                               GCGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAA                               >  SRR4302540.3141591/1‑94 (MQ=60)
                                                                CGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAAC                              >  SRR4302540.3888296/1‑94 (MQ=60)
                                                                CGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAAC                              >  SRR4302540.9403338/1‑94 (MQ=60)
                                                                CGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAAC                              <  SRR4302540.4607402/94‑1 (MQ=60)
                                                                CGTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGCCAAAAC                              >  SRR4302540.5478355/1‑94 (MQ=60)
                                                                 GTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACA                             >  SRR4302540.6698337/1‑94 (MQ=60)
                                                                 GTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGAACAGACTCTGCTGAATCTGGCAAAACA                             >  SRR4302540.2644659/1‑94 (MQ=60)
                                                                 GTCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGAAGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACA                             <  SRR4302540.8734341/94‑1 (MQ=60)
                                                                  TCGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAA                            >  SRR4302540.3201215/1‑94 (MQ=60)
                                                                   CGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAAC                           >  SRR4302540.7224220/1‑94 (MQ=60)
                                                                   CGTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAAC                           >  SRR4302540.5012138/1‑94 (MQ=60)
                                                                    GTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACC                          >  SRR4302540.5058530/1‑94 (MQ=60)
                                                                    GTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACC                          >  SRR4302540.8376211/1‑94 (MQ=60)
                                                                    GTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACC                          <  SRR4302540.2715441/94‑1 (MQ=60)
                                                                    GTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACC                          <  SRR4302540.2033624/94‑1 (MQ=60)
                                                                    GTCTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACC                          <  SRR4302540.10155345/94‑1 (MQ=60)
                                                                      atctagcgcttcgttGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAG                        <  SRR4302540.8670012/79‑1 (MQ=60)
                                                                      CTGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAG                        <  SRR4302540.2306279/94‑1 (MQ=60)
                                                                       TGCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGA                       >  SRR4302540.6710866/1‑94 (MQ=60)
                                                                       CGCAAGTCCCTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGA                       <  SRR4302540.8406063/94‑1 (MQ=60)
                                                                        GCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGAC                      <  SRR4302540.7328891/94‑1 (MQ=60)
                                                                        GCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGAC                      <  SRR4302540.4935567/94‑1 (MQ=60)
                                                                        GCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGAC                      <  SRR4302540.1268298/94‑1 (MQ=60)
                                                                        GCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGAC                      <  SRR4302540.4487650/94‑1 (MQ=60)
                                                                        GCAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGAC                      <  SRR4302540.1907874/94‑1 (MQ=60)
                                                                         CAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACG                     <  SRR4302540.3632710/94‑1 (MQ=60)
                                                                         CAAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACG                     <  SRR4302540.4226728/94‑1 (MQ=60)
                                                                          AAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGC                    >  SRR4302540.8290610/1‑94 (MQ=60)
                                                                          AAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGC                    >  SRR4302540.7540332/1‑94 (MQ=60)
                                                                          AAGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCACACGC                    >  SRR4302540.8357239/1‑94 (MQ=60)
                                                                           AGTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCT                   <  SRR4302540.5385247/94‑1 (MQ=60)
                                                                            GTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTA                  >  SRR4302540.3999172/1‑94 (MQ=60)
                                                                            GTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTA                  <  SRR4302540.8126206/94‑1 (MQ=60)
                                                                            GTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTA                  <  SRR4302540.2062488/94‑1 (MQ=60)
                                                                            GTCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTA                  >  SRR4302540.2315022/1‑94 (MQ=60)
                                                                            GTAACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTA                  <  SRR4302540.2706392/94‑1 (MQ=60)
                                                                             TCACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTAT                 <  SRR4302540.8886174/94‑1 (MQ=60)
                                                                              CACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATG                <  SRR4302540.3728715/94‑1 (MQ=60)
                                                                              CACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATG                <  SRR4302540.1332329/94‑1 (MQ=60)
                                                                              CACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATG                <  SRR4302540.1332328/94‑1 (MQ=60)
                                                                              CACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATG                <  SRR4302540.1458368/94‑1 (MQ=60)
                                                                              CACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATG                <  SRR4302540.562556/94‑1 (MQ=60)
                                                                              CACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATG                <  SRR4302540.4783809/94‑1 (MQ=60)
                                                                              CACTTCAGAGAAACCGGGCAACCTGGCAGTCCTCGACGTTACAGCCCGCGTTGCACAGACTCTGCTGAATCTGCCAAAACAACCAGACGCTATG                <  SRR4302540.10003035/94‑1 (MQ=53)
                                                                               ACTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGA               >  SRR4302540.1120875/1‑94 (MQ=60)
                                                                                CTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGAC              <  SRR4302540.3599164/94‑1 (MQ=60)
                                                                                CTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGAC              <  SRR4302540.308703/94‑1 (MQ=60)
                                                                                CTTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGAC              >  SRR4302540.6724482/1‑94 (MQ=60)
                                                                                 TTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGCATCTGGCAAAACAACCAGACGCTATGACT             >  SRR4302540.3776327/1‑94 (MQ=60)
                                                                                 TTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACT             >  SRR4302540.7675042/1‑94 (MQ=60)
                                                                                 TTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACT             >  SRR4302540.2125088/1‑94 (MQ=60)
                                                                                 TTCAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACT             <  SRR4302540.6315175/94‑1 (MQ=60)
                                                                                   CAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCA           <  SRR4302540.5523613/94‑1 (MQ=60)
                                                                                   CAGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCA           <  SRR4302540.7895632/94‑1 (MQ=60)
                                                                                    AGAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAC          >  SRR4302540.690565/1‑94 (MQ=60)
                                                                                     GAGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAAACAGACGCTATGACTCACC         >  SRR4302540.2449506/1‑94 (MQ=60)
                                                                                      AGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCC        <  SRR4302540.2413228/94‑1 (MQ=60)
                                                                                      AGAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCC        >  SRR4302540.9386810/1‑94 (MQ=60)
                                                                                       GAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCG       >  SRR4302540.3013609/1‑94 (MQ=60)
                                                                                       GAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCG       >  SRR4302540.5956964/1‑94 (MQ=60)
                                                                                       GAAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCG       >  SRR4302540.7529218/1‑94 (MQ=60)
                                                                                        AAAGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGG      <  SRR4302540.9760016/94‑1 (MQ=60)
                                                                                          AGCGGGCAACCTGGCGTTCCTCGACGTGAGGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGAC    <  SRR4302540.8423449/94‑1 (MQ=60)
                                                                                          AGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGAC    <  SRR4302540.1968897/94‑1 (MQ=60)
                                                                                          AGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGAC    <  SRR4302540.390819/94‑1 (MQ=60)
                                                                                          AGCGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATGGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGAC    <  SRR4302540.2875733/94‑1 (MQ=60)
                                                                                            CGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGTACATACTCTGCTGATTCTGGCAAAACAACCAGATGCTATGACTCATCCGGACGG  >  SRR4302540.5808846/1‑94 (MQ=60)
                                                                                            CGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGG  <  SRR4302540.2146259/94‑1 (MQ=60)
                                                                                            |                                                                                             
AAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGG  >  NC_000913/3484059‑3484244

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑