Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,510,121 +G intergenic (‑358/+151) yhfZ ← / ← trpS hypothetical protein/tryptophan tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,510,1201.G95.7% 573.7 / 17.5 186intergenic (‑357/+152)yhfZ/trpShypothetical protein/tryptophan tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base . (4/4);  new base G (117/61);  total (121/65)
Fisher's exact test for biased strand distribution p-value = 4.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01

Only 100 of 203 total aligned reads displayed.

AAGATAAGGCGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCC‑C‑GTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAGG  >  NC_000913/3510028‑3510209
                                                                                              |                                                                                         
aaGATAAGGCGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTccc‑c                                                                                            >  1:2761424/1‑93 (MQ=255)
  gATAAGGCGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCC‑C‑gg                                                                                         >  1:4758115/1‑92 (MQ=255)
   aTAAGGCGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCC‑C‑ggt                                                                                        <  1:6038408/93‑3 (MQ=255)
    tAAGGCGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCC‑C‑Ggtt                                                                                       >  2:6083263/1‑93 (MQ=255)
    tAAGGCGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCC‑C‑Ggtt                                                                                       <  2:4479559/93‑1 (MQ=255)
     aaGGCGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCC‑C‑Ggttt                                                                                      <  1:1020975/93‑2 (MQ=255)
          gcAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCagag                                                                                  >  2:3969419/1‑93 (MQ=255)
          gcAACAGCGAGATGTGAAAACGGCGAGGCACTTCACATTTTTGCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCGG‑GTTTCagag                                                                                  <  2:1511309/93‑1 (MQ=255)
             aCAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGtga                                                                               <  2:5425650/93‑1 (MQ=255)
             aCAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGtga                                                                               >  1:3886279/1‑93 (MQ=255)
             aCAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGtga                                                                               >  2:4684741/1‑93 (MQ=255)
                  gagaTGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATa                                                                          >  1:5607332/1‑93 (MQ=255)
                  gagaTGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATa                                                                          >  1:654808/1‑93 (MQ=255)
                   agaTGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATaa                                                                         >  2:5875149/1‑93 (MQ=255)
                    gaTGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCAG‑GTTTCAGAGTGATGATaaa                                                                        >  1:3116109/1‑93 (MQ=255)
                    gaTGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATaaa                                                                        >  2:5549906/1‑93 (MQ=255)
                    gaTGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATaaa                                                                        >  1:5636737/1‑93 (MQ=255)
                      tgtgGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAg                                                                      >  1:457894/1‑93 (MQ=255)
                      tgtgGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAg                                                                      >  1:1104706/1‑93 (MQ=255)
                       gtgGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGc                                                                     <  1:2622000/93‑1 (MQ=255)
                       gtgGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGc                                                                     >  1:3781581/1‑93 (MQ=255)
                       gtgGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGc                                                                     >  1:134313/1‑93 (MQ=255)
                        tgGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCa                                                                    >  2:2582461/1‑93 (MQ=255)
                          gAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTCTCCC‑CGGTTTCAGAGTGATGATAAAAGCaaa                                                                  <  1:5212630/93‑1 (MQ=255)
                            aaCGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAAt                                                                >  2:5836792/1‑93 (MQ=255)
                             aCGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAAtt                                                               <  2:3160146/93‑1 (MQ=255)
                               ggCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGc                                                             >  1:3848631/1‑93 (MQ=255)
                                gCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGcc                                                            <  2:5704415/93‑1 (MQ=255)
                                 cGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCt                                                           <  1:3478595/93‑1 (MQ=255)
                                 cGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCt                                                           <  1:5099447/93‑1 (MQ=255)
                                 cGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCt                                                           <  2:4098656/93‑1 (MQ=255)
                                 cGAGGCACTTCACATTTTTTCGGATTAGTGCAAATGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCt                                                           <  2:4579755/93‑1 (MQ=255)
                                   aGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGa                                                         >  2:3305843/1‑93 (MQ=255)
                                   aGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGa                                                         >  2:3856278/1‑93 (MQ=255)
                                    ggCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGAt                                                        >  2:3076350/1‑93 (MQ=255)
                                       aCTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATgcg                                                     >  1:1477407/1‑93 (MQ=255)
                                       aCTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATgcg                                                     <  2:5562334/93‑1 (MQ=255)
                                       aCTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATgcg                                                     >  2:4627981/1‑93 (MQ=255)
                                         ttCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCt                                                   <  1:554027/93‑1 (MQ=255)
                                         ttCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCt                                                   >  1:3757086/1‑93 (MQ=255)
                                         ttCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCt                                                   >  2:5029983/1‑93 (MQ=255)
                                           cacaTTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTAc                                                 <  1:4901806/93‑1 (MQ=255)
                                           cacaTTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTAc                                                 >  2:1023892/1‑93 (MQ=255)
                                              aTTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCt                                              >  1:5181484/1‑93 (MQ=255)
                                              aTTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCt                                              <  1:4652400/93‑1 (MQ=255)
                                              aTTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCt                                              >  1:4364587/1‑93 (MQ=255)
                                              aTTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCt                                              <  1:3344271/93‑1 (MQ=255)
                                                 ttttCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTAt                                           >  2:1550796/1‑93 (MQ=255)
                                                  tttCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATc                                          <  2:3510750/93‑1 (MQ=255)
                                                  tttCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCC‑CGGGTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATc                                          >  1:2853863/1‑93 (MQ=255)
                                                    tCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAg                                        <  1:6277616/93‑1 (MQ=255)
                                                     cGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAgg                                       >  2:3465445/1‑93 (MQ=255)
                                                      ggATTATTGCAAATGAGGATAAAGAACCCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAgga                                      <  2:3247821/93‑2 (MQ=37)
                                                      ggATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGc                                      >  2:2573045/1‑93 (MQ=255)
                                                      ggATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGc                                      >  1:30495/1‑93 (MQ=255)
                                                      ggATTATTGCAAATGAGGAAAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGc                                      >  2:6151420/1‑93 (MQ=255)
                                                       gATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGcc                                     <  1:1450263/93‑1 (MQ=255)
                                                        attattGCAAATGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCt                                    >  2:2891230/1‑93 (MQ=255)
                                                         ttattGCAAATGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTa                                   >  2:6192196/1‑93 (MQ=255)
                                                           attGCAAATGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACa                                 <  1:1174521/93‑1 (MQ=255)
                                                           attGCAAATGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACa                                 >  2:4852907/1‑93 (MQ=255)
                                                             tGCAAATGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACAtt                               >  2:2731287/1‑93 (MQ=255)
                                                             tGCAAATGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACAtt                               <  2:300576/93‑1 (MQ=255)
                                                             tGCAAATGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACAtt                               <  2:639172/93‑1 (MQ=255)
                                                              gCAAATGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACAttt                              >  1:5354795/1‑93 (MQ=255)
                                                                aaaTGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTcc                            >  2:1859883/1‑93 (MQ=255)
                                                                aaaTGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTcc                            >  1:5493394/1‑93 (MQ=255)
                                                                aaaTGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTcc                            >  1:921274/1‑93 (MQ=255)
                                                                 aaTGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCt                           >  2:3996934/1‑93 (MQ=255)
                                                                 aaTGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCt                           >  2:1462210/1‑93 (MQ=255)
                                                                 aaTGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCt                           >  1:2081218/1‑93 (MQ=255)
                                                                  aTGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCtt                          >  2:2067794/1‑93 (MQ=255)
                                                                  aTGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCtt                          >  1:4113999/1‑93 (MQ=255)
                                                                   tGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTg                         >  1:2231185/1‑93 (MQ=255)
                                                                   tGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTg                         >  2:1987235/1‑93 (MQ=255)
                                                                   tGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTg                         >  2:1882512/1‑93 (MQ=255)
                                                                    gAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCTCTACGCTTATCAGGCCTACATTTCCTTGc                        <  1:1639425/93‑1 (MQ=255)
                                                                    gAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGc                        >  1:2434138/1‑93 (MQ=255)
                                                                    gAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGc                        >  2:1169077/1‑93 (MQ=255)
                                                                    gAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGc                        >  2:4236874/1‑93 (MQ=255)
                                                                    gAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGc                        >  1:5430756/1‑93 (MQ=255)
                                                                       gATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAat                     >  2:2134121/1‑93 (MQ=255)
                                                                       gATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAat                     >  1:1673233/1‑93 (MQ=255)
                                                                        aTAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAata                    >  2:3609721/1‑93 (MQ=255)
                                                                        aTAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAata                    >  1:6157571/1‑93 (MQ=255)
                                                                          aaaaaaaCCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATAtg                  <  2:4975666/93‑1 (MQ=255)
                                                                          aaaaaaaCCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATAtg                  >  1:890504/1‑93 (MQ=255)
                                                                           aaaaaaCCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATAtgt                 <  2:172216/93‑1 (MQ=255)
                                                                            aaaaaCCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATAtgtg                >  2:693736/1‑93 (MQ=255)
                                                                            aaaaaCCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATAtgtg                >  2:6254058/1‑93 (MQ=255)
                                                                              aaaCCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCa              <  2:2194933/93‑1 (MQ=255)
                                                                              aaaCCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCa              <  2:4354352/93‑1 (MQ=255)
                                                                              aaaCCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCa              <  1:3248645/93‑1 (MQ=255)
                                                                                aCCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCAtt            <  2:5093413/93‑1 (MQ=255)
                                                                                    ggTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACtt        >  1:1869882/1‑93 (MQ=255)
                                                                                    ggTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACtt        <  1:5487124/93‑1 (MQ=255)
                                                                                      tttCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTg      >  2:5967733/1‑93 (MQ=255)
                                                                                       ttCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGt     >  2:3360918/1‑93 (MQ=255)
                                                                                       tcccc‑g‑GTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTa    <  2:678380/91‑1 (MQ=255)
                                                                                        cccc‑g‑GTTTCAGAGTGATGATAAAAGCAAAATTGCCCGATGCGCTACGCTTATCAGGCCTACATTTTCTTGCAATATGTGCATTACTTTTTAg   >  2:2309818/2‑93 (MQ=25)
                                                                                              |                                                                                         
AAGATAAGGCGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCC‑C‑GTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAGG  >  NC_000913/3510028‑3510209

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AAGATAAGGCGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCC‑C‑GTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAGGCCGGA  >  NC_000913/3510028‑3510214
                                                                                               |                                                                                             
AAGATAAGGCGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCC‑C‑G                                                                                               <  SRR4302533.638108/94‑1 (MQ=60)
 AGATAAGGCGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCC‑C‑GG                                                                                              >  SRR4302533.4786982/1‑94 (MQ=60)
 AGATAAGGCGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCC‑C‑GG                                                                                              <  SRR4302533.1989040/94‑1 (MQ=60)
   ATAAGGCGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCC‑C‑GGTT                                                                                            <  SRR4302533.6075000/94‑1 (MQ=60)
   ATAAGGCGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCC‑C‑GGTT                                                                                            >  SRR4302533.5444517/1‑94 (MQ=60)
   ATAAGGCGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCC‑C‑GGTT                                                                                            >  SRR4302533.5065877/1‑94 (MQ=60)
    TAAGGCGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCATATGAGGATAAAAAAACCGGGTTTCCC‑CGGTTT                                                                                            >  SRR4302533.306078/1‑94 (MQ=60)
     AAGGCGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTC                                                                                           <  SRR4302533.1026972/94‑1 (MQ=60)
        GCGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGA                                                                                        >  SRR4302533.1091823/1‑94 (MQ=60)
          GCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGT                                                                                      >  SRR4302533.914026/1‑94 (MQ=60)
           CAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTG                                                                                     >  SRR4302533.1795345/1‑94 (MQ=60)
            AACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGA                                                                                    >  SRR4302533.3909987/1‑94 (MQ=60)
            AACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGA                                                                                    >  SRR4302533.1662332/1‑94 (MQ=60)
                GCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGAT                                                                                <  SRR4302533.2484297/94‑1 (MQ=60)
                 CGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATA                                                                               >  SRR4302533.658674/1‑94 (MQ=60)
                 CGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATA                                                                               >  SRR4302533.5641216/1‑94 (MQ=60)
                   AGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCAG‑GTTTCAGAGTGATGATAAA                                                                             >  SRR4302533.3135267/1‑94 (MQ=60)
                   AGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAA                                                                             <  SRR4302533.335218/94‑1 (MQ=60)
                   AGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAA                                                                             >  SRR4302533.5670820/1‑94 (MQ=60)
                     ATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAG                                                                           >  SRR4302533.1111251/1‑94 (MQ=60)
                     ATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAG                                                                           >  SRR4302533.3670034/1‑94 (MQ=60)
                     ATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAG                                                                           >  SRR4302533.3427043/1‑94 (MQ=60)
                     ATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAG                                                                           >  SRR4302533.460632/1‑94 (MQ=60)
                     ATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAG                                                                           >  SRR4302533.2270891/1‑94 (MQ=60)
                      TGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGC                                                                          >  SRR4302533.135164/1‑94 (MQ=60)
                      TGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGC                                                                          >  SRR4302533.6198644/1‑94 (MQ=60)
                      TGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGC                                                                          >  SRR4302533.3804743/1‑94 (MQ=60)
                       GTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCA                                                                         <  SRR4302533.2638094/94‑1 (MQ=60)
                       GTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCA                                                                         >  SRR4302533.5224116/1‑94 (MQ=60)
                       GTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCA                                                                         >  SRR4302533.6158593/1‑94 (MQ=60)
                          GAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAA                                                                      <  SRR4302533.4667769/94‑1 (MQ=60)
                          GAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTCTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAA                                                                      <  SRR4302533.5244020/94‑1 (MQ=60)
                            AACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATT                                                                    >  SRR4302533.378487/1‑94 (MQ=60)
                              CGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGC                                                                  >  SRR4302533.3872132/1‑94 (MQ=60)
                               GGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCC                                                                 <  SRR4302533.5663856/94‑1 (MQ=60)
                                GCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCT                                                                >  SRR4302533.3181571/1‑94 (MQ=60)
                                 CGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTG                                                               <  SRR4302533.3500058/94‑1 (MQ=60)
                                 CGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTG                                                               <  SRR4302533.5130255/94‑1 (MQ=60)
                                     GCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGC                                                           >  SRR4302533.4450296/1‑94 (MQ=60)
                                      CACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCG                                                          >  SRR4302533.1486335/1‑94 (MQ=60)
                                       ACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGC                                                         >  SRR4302533.2155372/1‑94 (MQ=60)
                                        CTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCT                                                        >  SRR4302533.1587187/1‑94 (MQ=60)
                                        CTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCT                                                        >  SRR4302533.1242249/1‑94 (MQ=60)
                                        CTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCT                                                        >  SRR4302533.3780117/1‑94 (MQ=60)
                                         TTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTA                                                       <  SRR4302533.5267821/94‑1 (MQ=60)
                                         TTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTA                                                       >  SRR4302533.3896806/1‑94 (MQ=60)
                                         TTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTA                                                       <  SRR4302533.557311/94‑1 (MQ=60)
                                         TTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTA                                                       >  SRR4302533.1466291/1‑94 (MQ=60)
                                           CACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACG                                                     >  SRR4302533.5463624/1‑94 (MQ=60)
                                           CACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACG                                                     <  SRR4302533.4931508/94‑1 (MQ=60)
                                             CATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCT                                                   >  SRR4302533.5212771/1‑94 (MQ=60)
                                             CATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCT                                                   >  SRR4302533.4390933/1‑94 (MQ=60)
                                              ATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTT                                                  <  SRR4302533.3364934/94‑1 (MQ=60)
                                              ATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTT                                                  <  SRR4302533.4680593/94‑1 (MQ=60)
                                                 TTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCC‑CGGGTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATC                                               >  SRR4302533.2871421/1‑94 (MQ=60)
                                                    TCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGG                                            <  SRR4302533.6315097/94‑1 (MQ=60)
                                                     CGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGC                                           >  SRR4302533.1384877/1‑94 (MQ=60)
                                                     CGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGC                                           >  SRR4302533.30701/1‑94 (MQ=60)
                                                      GGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCC                                          >  SRR4302533.4347111/1‑94 (MQ=60)
                                                      GGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCC                                          <  SRR4302533.5521368/94‑1 (MQ=60)
                                                       GATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCT                                         <  SRR4302533.1459014/94‑1 (MQ=60)
                                                         TTATTGCAAATGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTAC                                       >  SRR4302533.5135735/1‑94 (MQ=60)
                                                          TATTGCAAATGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACA                                      >  SRR4302533.983050/1‑94 (MQ=60)
                                                           ATTGCAAATGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACAT                                     <  SRR4302533.1181571/94‑1 (MQ=60)
                                                             TGCAAATGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTT                                   >  SRR4302533.5386935/1‑94 (MQ=60)
                                                             TGCAAATGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGACTGATGCGCTACGCTTATCAGGCCTACATTT                                   >  SRR4302533.1112395/1‑94 (MQ=60)
                                                              GCAAATGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTC                                  >  SRR4302533.5612912/1‑94 (MQ=60)
                                                               CAAATGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCC                                 >  SRR4302533.926683/1‑94 (MQ=60)
                                                               CAAATGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCC                                 >  SRR4302533.5526530/1‑94 (MQ=60)
                                                                AAATGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCT                                >  SRR4302533.3829305/1‑94 (MQ=60)
                                                                AAATGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCT                                >  SRR4302533.2094025/1‑94 (MQ=60)
                                                                 AATGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTT                               <  SRR4302533.668401/94‑1 (MQ=60)
                                                                 AATGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTT                               >  SRR4302533.4139000/1‑94 (MQ=60)
                                                                  ATGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTG                              >  SRR4302533.2244951/1‑94 (MQ=60)
                                                                   TGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGC                             >  SRR4302533.2449175/1‑94 (MQ=60)
                                                                   TGAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGC                             >  SRR4302533.5463463/1‑94 (MQ=60)
                                                                    GAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCTCTACGCTTATCAGGCCTACATTTCCTTGCA                            <  SRR4302533.1649351/94‑1 (MQ=60)
                                                                    GAGGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCA                            <  SRR4302533.2811115/94‑1 (MQ=60)
                                                                      GGATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAAT                          >  SRR4302533.1683346/1‑94 (MQ=60)
                                                                       GATAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATA                         >  SRR4302533.6194611/1‑94 (MQ=60)
                                                                         TAAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATG                       >  SRR4302533.895736/1‑94 (MQ=60)
                                                                          AAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGT                      <  SRR4302533.5836039/94‑1 (MQ=60)
                                                                          AAAAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGT                      >  SRR4302533.3099115/1‑94 (MQ=60)
                                                                            AAAAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGC                    >  SRR4302533.26474/1‑94 (MQ=60)
                                                                              AAACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCAT                  <  SRR4302533.3268676/94‑1 (MQ=60)
                                                                              AAACCGGGTGTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAGTTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCAT                  <  SRR4302533.5557600/94‑1 (MQ=60)
                                                                               AACCGGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATT                 <  SRR4302533.4027989/94‑1 (MQ=60)
                                                                                   GGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTT             >  SRR4302533.1881281/1‑94 (MQ=60)
                                                                                   GGGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTT             >  SRR4302533.4716716/1‑94 (MQ=60)
                                                                                    GGTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTT            <  SRR4302533.5520201/94‑1 (MQ=60)
                                                                                     GTTTCCC‑CGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTG           <  SRR4302533.5460668/94‑1 (MQ=60)
                                                                                         CCC‑G‑GTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAGGC      <  SRR4302533.4810361/94‑1 (MQ=60)
                                                                                          CC‑G‑GTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAGGCC     >  SRR4302533.302435/1‑94 (MQ=60)
                                                                                          CC‑G‑GTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAGGCC     <  SRR4302533.239477/94‑1 (MQ=60)
                                                                                           C‑G‑GTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAGGCCG    <  SRR4302533.1541372/94‑1 (MQ=60)
                                                                                           C‑G‑GTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAGGCCG    <  SRR4302533.3446594/94‑1 (MQ=60)
                                                                                             G‑GTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAGGCCGG   >  SRR4302533.5644828/1‑94 (MQ=60)
                                                                                               GTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAGGCCGGA  >  SRR4302533.5951469/1‑94 (MQ=60)
                                                                                               GTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAGGCCGGA  <  SRR4302533.5099504/94‑1 (MQ=60)
                                                                                               |                                                                                             
AAGATAAGGCGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCC‑C‑GTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAGGCCGGA  >  NC_000913/3510028‑3510214

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑