Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,566,875 A→G R29R (CGT→CGC)  glgC ← glucose‑1‑phosphate adenylyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,566,8750AG100.0% 516.5 / NA 167R29R (CGT→CGC) glgCglucose‑1‑phosphate adenylyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (120/47);  total (120/47)

Only 100 of 246 total aligned reads displayed.

AGACAGCGCAAAGTCGATAATGCGGAACTTACCGCCGAAGTGTACGGCCGGTTTTGCTCGCTTATTGGTTAAA‑TCCTTCAGGCGGG‑TACCACGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTAAGTGATCGTTCTTCTCTAAACTAACCATGA  >  NC_000913/3566785‑3566963
                                                                                            |                                                                                        
  aCAGCGCAAAGTCGATAATGCGGAACTTACCGCCGAAGTGTACGGCCGGTTTTGCTCGCTTATTGGTTAAA‑TCCTTCAGGCGGG‑TACCGCGtc                                                                                      <  2:4295380/93‑1 (MQ=255)
     gcgcAAAGTCGATAATGCGGAACTTACCGCCGAAGTGTACGGCCGGTTTTGCTCGCTTATTGGTTAAA‑TCCTTCAGGCGGG‑TACCGCGtcctc                                                                                   >  1:6248075/1‑93 (MQ=255)
      cgcAAAGTCGATAATGCGGAACTTACCGCCGAAGTGTACGGCCGGTTTTGCTCGCTTATTGGTTAAA‑TCCTTCAGGCGGG‑TACCGCGtcctcc                                                                                  <  1:2090520/93‑1 (MQ=255)
         aaaGTCGATAATGCGGAACTTACCGCCGAAGTGTACGGCCGGTTTTGCTCGCTTATTGGTTAAA‑TCCTTCAGGCGGG‑TACCGCGTCCTCCCGc                                                                               >  1:6165279/1‑93 (MQ=255)
          aaGTCGATAATGCGGAACTTACCGCCGAAGTGTACGGCCGGTTTTGCTCGCTTATTGGTTAAA‑TCCTTCAGGCGGG‑TACCGCGTCCTCCCGcc                                                                              >  2:5385915/1‑93 (MQ=255)
            gTCGATAATGCGGAACTTACCGCCGAAGTGTACGGCCGGTTTTGCTCGCTTATTGGTTAAA‑TCCTTCAGGCGGG‑TACCGCGTCCTCCCGCCAg                                                                            <  1:324429/93‑1 (MQ=255)
            gTCGATAATGCGGAACTTACCGCCGAAGTGTACGGCCGGTTTTGCTCGCTTATTGGTTAAA‑TCCTTCAGGCGGG‑TACCGCGTCCTCCCGCCAg                                                                            <  1:6066928/93‑1 (MQ=255)
            gTCGATAATGCGGAACTTACCGCCGAAGTGTACGGCCGGTTTTGCTCGCTTATTGGTTAAA‑CCCTTCAGGCGGG‑TACCGCGTCCTCCCGCCAg                                                                            <  1:1595893/93‑1 (MQ=255)
             tCGATAATGCGGAACTTACCGCCGAAGTGTACGGCCGGTTTTGCTCGCTTATTGGTTAAA‑TCCTTCAGGCGGG‑TACCGCGTCCTCCCGCCAGt                                                                           >  2:1613556/1‑93 (MQ=255)
               gATAATGCGGAACTTACCGCCGAAGTGTACGGCCGGTTTTGCTCGCTTATTGGTTAAA‑TCCTTCAGGCGGG‑TACCGCGTCCTCCCGCCAGTAt                                                                         >  1:1029919/1‑93 (MQ=255)
                 tAATGCGGATCTTACCGCCGAAGTGTACGGCTGTTTTTTCTCGCTTATGGGTTAAT‑TCCTTCAGGCGGG‑TCCCGGCTCCTCCCGCCAGTATCa                                                                       <  2:1730153/93‑1 (MQ=255)
                 tAATGCGGAACTTACCGCCGAAGTGTACGGCCGGTTTTGCTCGCTTATTGTTTAAA‑TCCTTCAGGCGGG‑TACCGCGTCCTCCCGCCAGTATCa                                                                       <  1:5861393/93‑1 (MQ=255)
                 tAATGCGGAACTTACCGCCGAAGTGTACGGCCGGTTTTGCTCGCTTATTGGTTAAA‑TCCTTCAGGCGGG‑TACCGCGTCCTCCCGCCAGTATCa                                                                       <  2:1840569/93‑1 (MQ=255)
                   aTGCGGAACTTACCGCCGAAGTGTACGGCCGGTTTTGCTCGCTTATTGGTTAAA‑TCCTTCAGGCGGG‑TACCGCGTCCTCCCGCCAGTATCAgg                                                                     <  1:2155322/93‑1 (MQ=255)
                    tGCGGAACTTACCGCCGAAGTGTACGGCCGGTTTTGCTCGCTTATTGGTTAAA‑TCCTTCAGGCGGG‑TACCGCGTCCTCCCGCCAGTATCAggg                                                                    >  1:2557049/1‑93 (MQ=255)
                    tGCGGAACTTACCGCCGAAGTGTACGGCCGGTTTTGCTCGCTTATTGGTTAAA‑TCCTTCAGGCGGG‑TACCGCGTCCTCCCGCCAGTATCAggg                                                                    >  1:3070101/1‑93 (MQ=255)
                      cGGAACTTACCGCCGAAGTGTACGGCCGGTTTTGCTCGCTTATTGGTTAAA‑TCCTTCAGGCGGG‑TACCGCGTCCTCCCGCCAGTATCAGGGCa                                                                  >  2:478471/1‑93 (MQ=255)
                      cGGAACTTACCGCCGAAGTGTACGGCCGGTTTTGCTCGCTTATTGGTTAAA‑TCCTTCAGGCGGG‑TACCGCGTCCTCCCGCCAGTATCAGGGCa                                                                  >  1:5447612/1‑93 (MQ=255)
                       ggAACTTACCGCCGAAGTGTACGGCCGGTTTTGCTCGCTTATTGGTTAAA‑TCCTTCAGGCGGG‑TACCGCGTCCTCCCGCCAGTATCAGGGCaa                                                                 >  2:3657208/1‑93 (MQ=255)
                          aCTTACCGCCGAAGTGTACGGCCGGTTTTGCTCGCTTATTGGTTAAA‑TCCTTCAGGCGGG‑TACCGCGTCCTCCCGCCAGTATCAGGGCAACAg                                                              >  1:1015317/1‑93 (MQ=255)
                           cTTACCGCCGAAGTGTACGGCCGGTTTTGCTCGCTTATTGGTTAAA‑TCCTTCAGGCGGG‑TACCGCGTCCTCCCGCCAGTATCAGGGCAACAGa                                                             >  2:3227164/1‑93 (MQ=255)
                             tACCGCCGAAGTGTACGGCCGGTTTTGCTCGCTTATTGGTTAAA‑TCCTTCAGGCGGG‑TACCGCGTCCTCCCGCCAGTATCAGGGCAACAGAtt                                                           >  1:82929/1‑93 (MQ=255)
                               ccgccgAAGTGTACGGCCGGTTTTGCTCGCTTATTGGTTAAA‑TCCTTCAGGCGGG‑TACCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTc                                                         >  2:2812244/1‑93 (MQ=255)
                                 gccgAAGTGTACGGCCGTTTTTGCTCGCTTATGGGTTAAA‑TCCTTCGGGCGGG‑TCCCGCGCCCTCCCGCCAGTATCAGGGCAACAGATTTCaa                                                       <  2:64421/93‑1 (MQ=255)
                                   cgAAGTGTACGGCCGGTTTTGCTCGCTTATTGGTTAAA‑TCCTTCAGGCGGG‑TACCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATg                                                     >  1:43466/1‑93 (MQ=255)
                                    gAAGTGTACGGCCGGTTTTGCTCGCTTATTGGTTAAA‑TCCTTCAGGCGGG‑TACCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATgg                                                    >  2:3881164/1‑93 (MQ=255)
                                    gAAGTGTACGGCCGGTTTTGCTCGCTTATTGGTTAAA‑TCCTTCAGGCGGG‑TACCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATgg                                                    >  2:1370091/1‑93 (MQ=255)
                                     aaGTGTACGGCCGGTTTTGCTCGCTTATTGGTTAAA‑TCCTTCAGGCGGG‑TACCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGc                                                   >  2:4166228/1‑93 (MQ=255)
                                      aGTGTACGGCCGGTTTTGCTCGCTTATTGGTTAAA‑TCCTTCAGGCGGG‑TACCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCa                                                  >  1:4879220/1‑93 (MQ=255)
                                       gtgtATGGCCGGTTTTGCTCGCTTATTGGTTAAA‑TCCTTCAGGCGGG‑TCCCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAg                                                 <  1:1108287/93‑1 (MQ=255)
                                       gtgtAGGGCGGGTTTTGCTCGCTTATTGTTTAAA‑TCCTTCAGGCGGG‑TACCGCGCCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAg                                                 <  1:2591607/93‑1 (MQ=255)
                                        tgtgCGGCGGGTTTTGCGCGCTTATTGGTTAAA‑TCCTTGAGGCGGA‑TCCCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGc                                                <  1:5622595/93‑1 (MQ=255)
                                        tgtACGGCCGGTTTTGCTCGCTTATTGGTTAAA‑TCCTTCAGGCGGG‑TACCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGc                                                >  1:75096/1‑93 (MQ=255)
                                        tgtACGGCCGGTTTTGCTCGCTTATTGGTTAAA‑TCCTTCAGGCGGG‑TACCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGc                                                >  2:1742685/1‑93 (MQ=255)
                                         gtACGGCCGGTTTTGCTCGCTTATTGGTTAAA‑TCCTTCAGGCGGG‑TACCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGcg                                               >  2:1518304/1‑92 (MQ=255)
                                         gtACGGCCGGTTTTGCTCGCTTATTGGTTAAA‑TCCTTCAGGCGGG‑TACCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCt                                               <  1:4197569/93‑1 (MQ=255)
                                          tACGGCCGGTTTTGCTCGCTTATTGGTTAAA‑TCCTTCAGGCGGG‑TACCGCGTCCTCCCGCCAGTATCAGGGCCACAGATTTCAATGGCAGCTg                                              >  1:3418710/1‑93 (MQ=255)
                                           aCGGCCGGTTTTGCTCGCTTATTGGTTAAA‑TCCTTCAGGCGGG‑TCCCGCGCCCCCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTgg                                             <  2:730753/93‑1 (MQ=255)
                                            cggcggTTTTTCCTCACTTATGGTTGAAC‑TCCTTCGGGGGGT‑TACCGCCGCTACCCGCTAGTATCAGGGCAACAGATTTCAATGGCAGCTGgc                                            <  2:1002491/93‑1 (MQ=255)
                                             ggCCGGTTTTGCTCGCTTATTGGTTAAA‑TCCTTCAGGCGGG‑TACCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGgcg                                           >  2:2384603/1‑93 (MQ=255)
                                              gCCGGTTTTGCTCGCTTATTGGTTAAA‑TCCTTCAGGCGGG‑TACCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGgcgc                                          >  2:93238/1‑93 (MQ=255)
                                                 ggTTTTGCTCGCTTATTGGTTAAA‑TCCTTCAGGCGGG‑TACCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGcc                                       >  2:3460618/1‑93 (MQ=255)
                                                  gTTTTGCTCGCTTATTGGTTAAA‑TCCTTCAGGCGGG‑TACCGCGTCCTCCCGCCAGTATCAGGGCAACAGAATTTAAAGGGAGACGGGGCGccc                                      >  2:5908172/1‑92 (MQ=255)
                                                   ttttGCTCGCTTATTGGTTAAA‑TCCTTCAGGCGGG‑TACCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCaa                                     >  1:5412157/1‑93 (MQ=255)
                                                   ttttGCTCGCTTATTGGTTAAA‑TCCTTCAGGCGGG‑TACCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCaa                                     >  1:2996851/1‑93 (MQ=255)
                                                   ttttGCTCGCTTATTGGTTAAA‑TCCTTCAGGCGGG‑TACCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCaa                                     >  2:5261598/1‑93 (MQ=255)
                                                    ttttCTCGTTTA‑TGGTTAAACTCCTTCGGGCGGG‑TCCCGCGTCCCCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAAc                                    <  2:5540880/93‑1 (MQ=255)
                                                       tcTCGCTTATTGGTTAAA‑TCCTTCAGGCGGG‑TACCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACAtt                                 <  2:960175/92‑1 (MQ=255)
                                                       gCTCGCTTATTGGTTAAA‑TCCTTCAGGCGGG‑TACCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACAtt                                 >  1:1509938/1‑93 (MQ=255)
                                                        cTCGCTTATTGGTTAAA‑TCCTTCAGGCGGG‑TACCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGGTGGCGCGCCAACATTa                                >  2:6197040/1‑93 (MQ=255)
                                                         tcctttATTGGTTAAA‑TCCTTCGGGCGGG‑TCCCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTaa                               <  1:3688149/89‑1 (MQ=255)
                                                         tCGCTTATTGGTTAAA‑TCCTTCAGGCGGG‑GACCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTaa                               >  1:1036959/1‑93 (MQ=255)
                                                         tCGATTATGGGTTAAC‑TCCTTCAGGCGGG‑TCCCGCGTCCCCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTaa                               <  1:3931035/93‑1 (MQ=255)
                                                         gcGCTTCTTTGTAAAA‑TCCTTCCGGCGGC‑TGCCGCGTCCCCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTaa                               <  2:4025279/92‑1 (MQ=255)
                                                         cctcTTATTGGTAAAT‑TCCTACAGGCGGG‑TCCCGCGCCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTaa                               <  2:2879262/90‑1 (MQ=255)
                                                          cGCTTATTGGTTAAA‑TCCTTCAGGCGGG‑TACCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTAAg                              >  1:1768878/1‑93 (MQ=255)
                                                           tcTTATTGGTTAAA‑TCCTTCAGGCGGG‑TACCGCGCCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTAAGt                             <  2:1084015/92‑1 (MQ=255)
                                                           gCTTATTGGTTAAA‑TCCTTCAGGCGGT‑TACCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTAAGt                             <  1:1836967/93‑1 (MQ=255)
                                                           ctttATTGTTTAAA‑TCCTTCAGGCGGG‑TACCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTAAGt                             <  1:5725210/91‑1 (MQ=255)
                                                            tttATTGTTAAAA‑TCTTCGGGGCGGT‑TCCCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTAAGTg                            <  1:5374547/92‑1 (MQ=255)
                                                            tttATTGGTTAAA‑TCCTTCAGGCGGG‑TCCCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTAAGTg                            <  1:2451435/92‑1 (MQ=255)
                                                            cTTATTGATTAAA‑TCCTTCAGGCGGG‑TCCCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTAAGTg                            <  1:1378615/93‑1 (MQ=255)
                                                              tATTGTTTAAA‑TCCTTCAGGCGGG‑TACCGCCTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTAAGTGAt                          <  2:3937064/93‑1 (MQ=255)
                                                              tATTGGTTAAA‑TCCTTCAGGCGGG‑TACCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTAAGTGAt                          >  2:4999619/1‑93 (MQ=255)
                                                               tttGTTTAAA‑CCCTCCAGGCGGG‑TACCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTAAGTGATc                         <  1:4689430/92‑1 (MQ=255)
                                                               aTTGGTTAAT‑CCCTTCAGGCGGG‑TACCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTAAGTGATc                         <  2:2091148/93‑1 (MQ=255)
                                                               aTTGGTTAAA‑TCCTTCAGGCGGG‑TACCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTAAGTGATc                         >  2:2242533/1‑93 (MQ=255)
                                                                tttgTTAAA‑TTCTTTGAGCGGGATCCCCCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTAAGTGATc                         <  2:3594552/90‑1 (MQ=255)
                                                                 tGGTTAAA‑TCCTTCAGGCGGG‑TCCCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTAAGTGATCGt                       <  1:4555645/93‑1 (MQ=255)
                                                                  ggTTAAA‑TCCTTCAGGCGGG‑TACCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTAAGTGATCGtt                      >  1:3523193/1‑93 (MQ=255)
                                                                   gTTAAT‑TCCTCGAGGCGGG‑TCCCGCGCCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTAAGTGATCGttc                     <  2:1227382/93‑1 (MQ=255)
                                                                    ttAAA‑TCCTTCAGGCGGG‑TCCCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTAAGTGATCGttct                    <  1:5549822/93‑1 (MQ=255)
                                                                     tAAA‑TCCTCCAGGCGGG‑TACCGCGCCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTAAGTGATCGttctt                   <  1:3343800/93‑1 (MQ=255)
                                                                      aaa‑TCCTTCAGGCGGG‑TACCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTAAGTGATCGttcttc                  >  2:468483/1‑93 (MQ=255)
                                                                      aaa‑TCCTTCAGGCGGG‑TACCGCGTCCCCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTAAGTGATCGttcttc                  <  1:5108444/93‑1 (MQ=255)
                                                                        actCCTTCAGGCGGG‑TCCCGCGCCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTAAGTGATCGTtcttct                 <  2:2549922/91‑1 (MQ=255)
                                                                           ccTTGAGGCGGT‑TCCCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTAAGTGATCGTTCTTCTCTa              <  1:3786894/93‑1 (MQ=255)
                                                                           ccTTCAGGCGGG‑TACCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTAAGTGATCGTTCTTCTCTa              >  1:961100/1‑93 (MQ=255)
                                                                           ccTTCAGGCGGG‑TACCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTAAGTGATCGTTCTTCTCTa              >  2:2700083/1‑93 (MQ=255)
                                                                             ttCAGGCGGG‑TACCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTAAGTGATCGTTCTTCTCTaaa            >  1:2723010/1‑93 (MQ=255)
                                                                             ttCAGGCGGG‑TACCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTAAGTGATCGTTCTTCTCTaaa            >  2:5834689/1‑93 (MQ=255)
                                                                              tCAGGCGGT‑GCCCGCGCCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTAAGTGATCGTTCTTCTCTAAAc           <  1:2696023/93‑1 (MQ=255)
                                                                              tCAGGCGGG‑TACCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTAAGTGATCGTTCTTCTCTAAAc           >  2:412777/1‑93 (MQ=255)
                                                                               cAGGCGGG‑TACCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTAAGTGATCGTTCTTCTCTAAACt          >  1:79720/1‑93 (MQ=255)
                                                                                gggCGGG‑TGCCGCGCCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTAAGTGATCGTTCTTCTCTAAACTa         <  1:2008164/92‑1 (MQ=255)
                                                                                aGGCGGG‑TACCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTAAGTGATCGTTCTTCTCTAAACTa         >  2:5079997/1‑93 (MQ=255)
                                                                                aGGCGGG‑TACCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTAAGTGATCGTTCTTCTCTAAACTa         >  2:3265195/1‑93 (MQ=255)
                                                                                aGGCGGG‑TACCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTAAGTGATCGTTCTTCTCTAAACTa         >  2:2339936/1‑93 (MQ=255)
                                                                                 ggCGGG‑TACCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTAAGTGATCGTTCTTCTCTAAACTaa        <  1:1073076/93‑1 (MQ=255)
                                                                                  gCGGG‑TCCCGCCCCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTAAGTGATCGTTCTTCTCTAAACTAAc       <  1:132165/93‑1 (MQ=255)
                                                                                  gCGGG‑TACCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTAAGTGATCGTTCTTCTCTAAACTAAc       >  2:3145217/1‑93 (MQ=255)
                                                                                   cGGG‑TCCCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTAAGTGATCGTTCTTCTCTAAACTAAcc      <  1:3632986/93‑1 (MQ=255)
                                                                                    ggg‑TCCCGCGCCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTAAGTGATCGTTCTTCTCTAAACTAACCa     <  2:3485510/93‑1 (MQ=255)
                                                                                    ggg‑TACCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCTCGCCAACATTAAGTGATCGTTCTTCTCTAAAATAATCa     >  1:234632/1‑93 (MQ=255)
                                                                                    ggg‑TACCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTAAGTGATCGTTCTTCTCTAAACTAACCa     >  2:5433265/1‑93 (MQ=255)
                                                                                    ggg‑TACCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTAAGTGATCGATCTTCTCTATACTCAccc     >  2:2628747/1‑92 (MQ=255)
                                                                                     gg‑TACCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTAAGTGATCGTTCTTCTCTAAACTAACCAt    >  2:5740210/1‑93 (MQ=255)
                                                                                     gg‑TACCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTAAGTGATCGTTCTTCTCTAAACTAACCAt    >  1:4962427/1‑93 (MQ=255)
                                                                                        tACCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTAAGTGATCGTTCTTCTCTAAACTAACCATGa  >  2:4390409/1‑93 (MQ=255)
                                                                                        tACCGCGCCCCCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTAAGTGATCGTTCTTCTCTAAACTAACCATGa  <  2:5198179/93‑1 (MQ=255)
                                                                                            |                                                                                        
AGACAGCGCAAAGTCGATAATGCGGAACTTACCGCCGAAGTGTACGGCCGGTTTTGCTCGCTTATTGGTTAAA‑TCCTTCAGGCGGG‑TACCACGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTAAGTGATCGTTCTTCTCTAAACTAACCATGA  >  NC_000913/3566785‑3566963

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTAGACAGCGCAAAGTCGATAATGCGGAACTTACCGCCGAAGTGTACGGCCGGTTTTGCTCGCTTATTGGTTAAA‑TCCTTCAGGCGGGTACCACGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTAAGTGATCGTTCTTCTCTAAACTAACCATGACTAA  >  NC_000913/3566783‑3566967
                                                                                             |                                                                                            
GTAGACAGCGCAAAGTCGATAATGCGGAACTTACCGCCGAAGTGTACGGCCGGTTTTGCTCGCTTATTGGTTAAA‑TCCTTCAGGCGGGTACCGC                                                                                             <  SRR4302533.250263/94‑1 (MQ=60)
 TAGACAGCGCAAAGTCGATAATGCGGAACTTACCGCCGAAGTGTACGGCCGGTTTTGCTCGCTTATTGGTTAAA‑TCCTTCAGGCGGGTACCGCG                                                                                            <  SRR4302533.5274204/94‑1 (MQ=60)
    ACAGCGCAAAGTCGATAATGCGGAACTTACCGCCGAAGTGTACGGCCGGTTTTGCTCGCTTATTGGTTAAA‑TCCTTCAGGCGGGTACCGCGTCC                                                                                         <  SRR4302533.4674937/94‑1 (MQ=60)
    ACAGCGCAAAGTCGATAATGCGGAACTTACCGCCGAAGTGTACGGCCGGTTTTGCTCGCTTATTGGTTAAA‑TCCTTCAGGCGGGTACCGCGTCC                                                                                         >  SRR4302533.813567/1‑94 (MQ=60)
      AGCGCAAAGTCGATAATGCGGAACTTACCGCCGAAGTGTACGGCCGGTTTTGCTCGCTTATTGGTTAAA‑TCCTTCAGGCGGGTACCGCGTCCTC                                                                                       >  SRR4302533.6285463/1‑94 (MQ=60)
        CGCAAAGTCGATAATGCGGAACTTACCGCCGAAGTGTACGGCCGGTTTTGCTCGCTTATTGGTTAAA‑TCCTTCAGGCGGGTACCGCGTCCTCCC                                                                                     <  SRR4302533.2103393/94‑1 (MQ=60)
        CGCAAAGTCGATAATGCGGAACTTACCGCCGAAGTGTACGGCCGGTTTTGCTCGCTTATTGGTTAAA‑TCCTTCAGGCGGGTACCGCGTCCTCCC                                                                                     <  SRR4302533.1378000/94‑1 (MQ=60)
         GCAAAGTCGATAATGCGGAGCTTACCGCCGAAGTGTACGGCCGGTTTTGCTCGCTTATTGGTTAAA‑TCCTTCAGGCGGGTACCGCGTCCTCCCG                                                                                    >  SRR4302533.4992268/1‑94 (MQ=60)
          CAAAGTCGATAATGCGGAACTTACCGCCGAAGTGTACGGCCGGTTTTGCTCGCTTATTGGTTAAA‑TCCTTCAGGCGGGTACCGCGTCCTCCCGC                                                                                   >  SRR4302533.6202340/1‑94 (MQ=60)
          CAAAGTCGATAATGCGGAACTTACCGCCGAAGTGTACGGCCGGTTTTGCTCGCTTATTGGTTAAA‑TCCTTCAGGCGGGTACCGCGTCCTCCCGC                                                                                   >  SRR4302533.3439632/1‑94 (MQ=60)
          CAAAGTCGATAATGCGGAACTTACCGCCGAAGTGTACGGCCGGTTTTGCTCGCTTATTGGTTAAA‑TCCTTCAGGCGGGTACCGCGTCCTCCCGC                                                                                   >  SRR4302533.3344153/1‑94 (MQ=60)
           AAAGTCGATAATGCGGAACTTACCGCCGAAGTGTACGGCCGGTTTTGCTCGCTTATTGGTTAAA‑TCCTTCAGGCGGGTACCGCGTCCTCCCGCC                                                                                  >  SRR4302533.696607/1‑94 (MQ=60)
           AAAGTCGATAATGCGGAACTTACCGCCGAAGTGTACGGCCGGTTTTGCTCGCTTATTGGTTAAA‑TCCTTCAGGCGGGTACCGCGTCCTCCCGCC                                                                                  >  SRR4302533.1025586/1‑94 (MQ=60)
             AGTCGATAATGCGGAACTTACCGCCGAAGTGTACGGCCGGTTTTGCTCGCTTATTGGTTAAA‑TCCTTCAGGCGGGTACCGCGTCCTCCCGCCAG                                                                                >  SRR4302533.6274574/1‑94 (MQ=60)
             AGTCGATAATGCGGAACTTACCGCCGAAGTGTACGGCCGGTTTTGCTCGCTTATTGGTTAAA‑TCCTTCAGGCGGGTACCGCGTCCTCCCGCCAG                                                                                >  SRR4302533.2537366/1‑94 (MQ=60)
              GTCGATAATGCGGAACTTACCGCCGAAGTGTACGGCCGGTTTTGCTCGCTTATTGGTTAAA‑TCCTTCAGGCGGGTACCGCGTCCTCCCGCCAGT                                                                               <  SRR4302533.6103644/94‑1 (MQ=60)
              GTCGATAATGCGGAACTTACCGCCGAAGTGTACGGCCGGTTTTGCTCGCTTATTGGTTAAA‑TCCTTCAGGCGGGTACCGCGTCCTCCCGCCAGT                                                                               <  SRR4302533.326408/94‑1 (MQ=60)
              GTCGATAATGCGGAACTTACCGCCGAAGTGTACGGCCGGTTTTGCTCGCTTATTGGTTAAA‑CCCTTCAGGCGGGTACCGCGTCCTCCCGCCAGT                                                                               <  SRR4302533.1605551/94‑1 (MQ=60)
               ccaataaggcgtaacttaccgccgattgtTACGGCCGGTTTTGCTCGCTTATTGGTAAAT‑TCCTTCAGGCGGGTACCGCGTCCTCCCGCCAGTA                                                                              <  SRR4302533.5756709/65‑1 (MQ=60)
                CGATAATGCGGAACTTACCGCCGAAGTGTACGGCCGGTTTTGCTCGCTTATTGGTTAAA‑TCCTTCAGGCGGGTACCGCGTCCTCCCGCCAGTAT                                                                             <  SRR4302533.284800/94‑1 (MQ=60)
                CGATAATGCGGAACTTACCGCCGAAGTGTACGGCCGGTTTTGCTCGCTTATTGGTTAAA‑TCCTTCAGGCGGGTACCGCGTCCTCCCGCCAGTAT                                                                             >  SRR4302533.1035973/1‑94 (MQ=60)
                 GATAATGCGGAACTTACCGCCGAAGTGTACGGCCGGTTTTGCTCGCTTATTGGTTAAA‑TCCTTCAGGCGGGTACCGCGTCCTCCCGCCAGTATC                                                                            >  SRR4302533.962304/1‑94 (MQ=60)
                 GATAATGCGGAACTTACCGCCGAAGTGTACGGCCGGTTTTGCTCGCTTATTGGTTAAA‑TCCTTCAGGCGGGTACCGCGTCCTCCCGCCAGTATC                                                                            >  SRR4302533.2582464/1‑94 (MQ=60)
                  ATAATGCGGAACTTACCGCCGAAGTGTACGGCCGGTTTTGCTCGCTTCTTGGTTAAA‑TCCTTCAGGCGGGTGACGCGTCCTCCCGCCAGcatcg                                                                           >  SRR4302533.973804/1‑89 (MQ=60)
                   TAATGCGGAACTTCCCGCCGAAGTGTACGGCCGGTTTTGCTCGCTTATTGTTTAAA‑TCCTTCAGGCGGTTACCGCGTCCTCCCGCCAGTATCAG                                                                          <  SRR4302533.3394096/94‑1 (MQ=60)
                   TAATGCGGAACTTACCGCCGAAGTGTACGGCCGGTTTTGCTCGCTTATTGTTTAAA‑TCCTTCAGGCGGGTACCGCGTCCTCCCGCCAGTATCAG                                                                          <  SRR4302533.5896833/94‑1 (MQ=60)
                   TAATGCGGAACTTACCGCCGAAGTGTACGGCCGGTTTTGCTCGCTTATTGGTTAAA‑TCCTTCAGGCGGGTACCGCGTCCTCCCGCCAGTATCAG                                                                          <  SRR4302533.2521929/94‑1 (MQ=60)
                    AATGCGGAACTTCCCGCCGAAGTGTACGGCCGGTTTTGCTCGCTTATTGGTTAAA‑TCCTTCAGGCGGGTACCGCGTCCTCCCGCCAGTATCAGG                                                                         <  SRR4302533.212482/94‑1 (MQ=60)
                     ATGCGGAACTTACCGCCGAAGTGTACGGCCGGTTTTGCTCGCTTATTGGTTAAA‑TCCTTCAGGCGGGTACCGCGTCCTCCCGCCAGTATCAGGG                                                                        >  SRR4302533.2572773/1‑94 (MQ=60)
                     ATGCGGAACTTACCGCCGAAGTGTACGGCCGGTTTTGCTCGCTTATTGGTTAAA‑TCCTTCAGGCGGGTACCGCGTCCTCCCGCCAGTATCAGGG                                                                        <  SRR4302533.1401761/94‑1 (MQ=60)
                     ATGCGGAACTTACCGCCGAAGTGTACGGCCGGTTTTGCTCGCTTATTGGTTAAA‑TCCTTCAGGCGGGTACCGCGTCCTCCCGCCAGTATCAGGG                                                                        <  SRR4302533.2168593/94‑1 (MQ=60)
                     ATGCGGAACTTACCGCCGAAGTGTACGGCCGGTTTTGCTCGCTTATTGGTTAAA‑TCCTTCAGGCGGGTACCGCGTCCTCCCGCCAGTATCAGGG                                                                        >  SRR4302533.3088949/1‑94 (MQ=60)
                       GCGGAACTTACCGCCGAAGTGTACGGCCGGTTTTGCTCGCTTATTGGTTAAA‑TCCTTCAGGCGGGTACCGCGTCCTCCCGCCAGTATCAGGGCA                                                                      >  SRR4302533.5480430/1‑94 (MQ=60)
                          GAACTTCCCGCGGAAGTGTACGGCCGGTTTTCCTCGCTTATTGGTTAAA‑TCCTTCAGGCGGGTCCCGCGTCCTCCCGCCAGTATCAGGGCAACA                                                                   <  SRR4302533.1944850/94‑1 (MQ=60)
                           AACTTACCGCCGAAGTGTACGGCCGGTTTTGCTCGCTTATTGGTTAAA‑TCCTTCAGGCGGGTACCGCGTCCTCCCGCCAGTATCAGGGCAACAG                                                                  >  SRR4302533.1021282/1‑94 (MQ=60)
                             CTAACCGCAGAGGTGTACGGCGGGTTTTGCTCGCTTTTTGGTTAAA‑TCCTACAGGCGGGTACCGCTTCCTCCCGCCAGTATCAGGGCAACAGAT                                                                <  SRR4302533.4773697/94‑1 (MQ=60)
                              TTACCGCCGAAGTGTACGGCCGGTTTTGCTCGCTTATTGGTTAAA‑TCCTTCAGGCGGGTACCGCGTCCTCCCGCCAGTATCAGGGCAACAGATT                                                               >  SRR4302533.83456/1‑94 (MQ=60)
                                ACCGCCGAAGTGTACGGCCGGTTTTGCTCGCTTATTGGTTAAA‑TCCTTCAGGCGGGTACCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTC                                                             >  SRR4302533.3541937/1‑94 (MQ=60)
                                 CCGCCGAAGTGTACGGCCGTTTTTGCTCGCTTATTGGTTAAA‑TCCTTCAGGCGGGTCCCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCA                                                            <  SRR4302533.3214160/94‑1 (MQ=60)
                                 CCGCCGAAGTGTACGGCCGGTTTTGCTCGCTTATTGGTTAAA‑TCCTTCAGGCGGGTACCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCA                                                            <  SRR4302533.764615/94‑1 (MQ=60)
                                   GCCGAAGTGTACGGCCGGTTTTGCTCGCTTATTGGTTAAA‑TCCTTCAGGCGGGTACCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAAT                                                          >  SRR4302533.1086098/1‑94 (MQ=60)
                                   CCCGAAGTGTGCGGCCCGTTGTGCTCGCTTATTGGTTAAA‑TCCTTCAGGCGGGTACCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAAT                                                          <  SRR4302533.4943810/94‑1 (MQ=60)
                                    CCGAGGTGTACGGCGGGTTTTGCTCGCTTATTGGTTAAA‑TCCTTCAGGCGGGTACCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATG                                                         <  SRR4302533.2238499/94‑1 (MQ=60)
                                    CCGAAGTGTACGGCCGGTTTTGCTCGCTTATTGGTTAAA‑TCCTTCAGGCGGGTACCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATG                                                         >  SRR4302533.43764/1‑94 (MQ=60)
                                     CGAAGTGTACGGCCGGTTTTGCTCGCTTATTGGTTAAA‑TCCTTCAGGCGGGTACCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGG                                                        >  SRR4302533.1408153/1‑94 (MQ=60)
                                       AAGTGTACGGCCGGTTTTGCTCGCTTATTGGTTAAA‑TCCTTCAGGCGGGTACCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCA                                                      >  SRR4302533.4908780/1‑94 (MQ=60)
                                       AAGTGTACGGCCGGTTTTGCTCGCTTATTGGTTAAA‑TCCTTCAGGCGGGTACCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCA                                                      >  SRR4302533.4908781/1‑94 (MQ=60)
                                        AGTGTACGGCGGGTTTTGCTCGCTTATTGGTTAAA‑CCCTTCAGGCGGGTACCGCGCCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAG                                                     <  SRR4302533.2181462/94‑1 (MQ=60)
                                        AGTGTACGGCCGGTTTTGCTCGCTTATTGGTTAAA‑TCCTTCAGGCGGGTACCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAG                                                     >  SRR4302533.1869599/1‑94 (MQ=60)
                                        AGTGTACGGCCGGTTTTGCTCGCTTATTGGTTAAA‑TCCTTCAGGCGGGTACCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAG                                                     >  SRR4302533.2691650/1‑94 (MQ=60)
                                         GTGTATGGCCGGTTTTGCTCGCTTATTGGTTAAA‑TCCTTCAGGCGGGTCCCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGC                                                    <  SRR4302533.1114860/94‑1 (MQ=60)
                                         GTGTAGGGCGGGTTTTGCTCGCTTATTGTTTAAA‑TCCTTCAGGCGGGTACCGCGCCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGC                                                    <  SRR4302533.2607532/94‑1 (MQ=60)
                                         GTGTACGGCCGGTTTTGCTCGCTTATTGGTTAAA‑TCCTTCAGGCGGGTACCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGC                                                    >  SRR4302533.75583/1‑94 (MQ=60)
                                          TTTACGGCCGGTTTTGCTCGCTTATTGTTTAAA‑TCCTTCAGGCGGGTACCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCT                                                   <  SRR4302533.1119027/94‑1 (MQ=60)
                                          TGTGCGGCGGGTTTTGCGCGCTTATTGGTTAAA‑TCCTTGAGGCGGATCCCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCT                                                   <  SRR4302533.5656579/94‑1 (MQ=60)
                                           gtatgtccgtTTTTGCTCGTTTATTGGTAAAA‑TCCTTCAGGCGGGTCCCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTG                                                  <  SRR4302533.2368566/84‑1 (MQ=60)
                                           gtaggcgcgtTTTTGCTCGCTTTTGGGTTAAA‑TCCTACAGGCGGGTACCGCGCCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTG                                                  <  SRR4302533.3488793/84‑1 (MQ=60)
                                           GTACGGCCGGTTTTGCTCGCTTATTGGTTAAA‑TCCTTCAGGCGGGTCCCGCGTCCCCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTG                                                  <  SRR4302533.315331/94‑1 (MQ=60)
                                           GTACGGCCGGTTTTGCTCGCTTATTGGTTAAA‑TCCTTCAGGCGGGTACCGCGTCCTCCCGCCAGTATCAGGGCCACAGATTTCAATGGCAGCTG                                                  >  SRR4302533.3439833/1‑94 (MQ=60)
                                           GTACGGCCGGTTTTGCTCGCTTATTGGTTAAA‑TCCTTCAGGCGGGTACCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTG                                                  <  SRR4302533.4222978/94‑1 (MQ=60)
                                              CGGCCGGTTTTGCTCGCTTATTGGTTAAA‑TCCTTCAGGCGGGTACCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCG                                               >  SRR4302533.171794/1‑94 (MQ=60)
                                                GCCGGTTTTGCTCGCTTATTGTTAAAC‑TCCTTCAGCCGGTTACCGCGCCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCG                                             <  SRR4302533.158266/94‑1 (MQ=60)
                                                 CCGGTTTTGCTCGCTTATTGGTTAAA‑TCCTTCAGGCGGGTACCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTtgagctc                                            >  SRR4302533.40417/1‑87 (MQ=60)
                                                 CCGGTTTTGCTCGCTTATTGGTTAAA‑TCCTTCAGGCGGGTACCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGC                                            >  SRR4302533.6132557/1‑94 (MQ=60)
                                                  CGGTTTTACTCGCTTATTGGTAAAA‑TCCTTCAGGCGGATACCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCC                                           <  SRR4302533.5535667/94‑1 (MQ=60)
                                                    GTTTTGCTCGCTTATTGGTTAAA‑TCCTTCAGGCGGGTACCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAA                                         >  SRR4302533.5444701/1‑94 (MQ=60)
                                                    GTTTTGCTCGCTTATTGGTTAAA‑TCCTTCAGGCGGGTACCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAA                                         >  SRR4302533.3015176/1‑94 (MQ=60)
                                                      TTTGCTCGCTTATTGGTTAAA‑TCCTTCAGGCGGGTACCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACA                                       >  SRR4302533.2021449/1‑94 (MQ=60)
                                                       TTGCTCGCTTATTGGTTAAA‑TCCTTCGGGCGGGTACCGCGTCCCCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACAT                                      <  SRR4302533.3771207/94‑1 (MQ=60)
                                                        TGCTCGCTTATTGTTTAAA‑TCCTTCAGGCGGGTCCCGCTTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATT                                     <  SRR4302533.796848/94‑1 (MQ=60)
                                                        TGCTCGCTTATTGGTTAAA‑TCCTTCAGGCGGGTACCGCGTCCTCCCGCCCGTATCAGGGCAACAGATTTCAATGGCAGGTGGCGCGCCAAAATT                                     >  SRR4302533.5344174/1‑94 (MQ=60)
                                                        TGCTCGCTTATTGGTTAAA‑TCCTTCAGGCGGGTACCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATT                                     >  SRR4302533.1519040/1‑94 (MQ=60)
                                                        TGCTCGCTTATTGGTAAAT‑TCCTTCGGGCGGCTCCCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATT                                     <  SRR4302533.5717404/94‑1 (MQ=60)
                                                         GCTCGCTTATTGGTTAAA‑TCCTTCAGGCGGGTCCCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTA                                    <  SRR4302533.3852879/94‑1 (MQ=60)
                                                         GCTCGCTTATTGGTTAAA‑TCCTTCAGGCGGGTACCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTA                                    >  SRR4302533.3692159/1‑94 (MQ=60)
                                                         GCTCGCTTATTGGTTAAA‑TCCTTCAGGCGGGTACCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTA                                    >  SRR4302533.1735937/1‑94 (MQ=60)
                                                          CTCGCTTATTGGTTAAA‑TCCTTCAGGCGGGGACCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTAA                                   >  SRR4302533.1043055/1‑94 (MQ=60)
                                                           tcctTTATTGGTTAAA‑TCCTTCGGGCGGGTCCCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTAAG                                  <  SRR4302533.3710814/90‑1 (MQ=60)
                                                           TCGTTTATTGGTTAAA‑TCCTCCAGGCGGGTCCCGCGTCCCCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTAAG                                  <  SRR4302533.1213157/94‑1 (MQ=60)
                                                           TCGCTTATTGGTTAAA‑TCCTTCAGGCGGGTACCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTAAG                                  >  SRR4302533.1779649/1‑94 (MQ=60)
                                                           TCGCTTATTGGTTAAA‑TCCTTCAGGCGGGTACCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTAAG                                  >  SRR4302533.1779648/1‑94 (MQ=60)
                                                           TCGCTTATTGGTTAAA‑TCCTTCAGGCGGGTACCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTAAG                                  <  SRR4302533.3665841/94‑1 (MQ=60)
                                                           TCGATTATGGGTTAAC‑TCCTTCAGGCGGGTCCCGCGTCCCCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTAAG                                  <  SRR4302533.3955032/94‑1 (MQ=60)
                                                            CGCTTATTGGTTAAA‑TCCTTCAGGCGGGTACCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCCTCTGGCGCGCCAACATTAAGG                                 >  SRR4302533.478058/1‑94 (MQ=60)
                                                             gttttTTGGTTAAA‑TCCTTCAGGCGGGTCCCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTAAGTG                                <  SRR4302533.4950606/89‑1 (MQ=60)
                                                             ctTTATTGTTTAAA‑TCCTTCAGGCGGGTACCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTAAGTG                                <  SRR4302533.5759774/92‑1 (MQ=60)
                                                             atttatgtGTTAAA‑CCCTTCAGGCGGGTCCCGCCGCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTAAGTG                                <  SRR4302533.1194356/86‑1 (MQ=60)
                                                             TCTTATTGGTTAAA‑TCCTTCAGGCGGGTCCCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTAAGTG                                <  SRR4302533.2911105/94‑1 (MQ=60)
                                                             GTTTATTGGTTAAA‑TCCTTCAGGCGGTTACCGCGCCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTAAGTG                                <  SRR4302533.5654114/94‑1 (MQ=60)
                                                             GCTTATTGGTTAAA‑TCCTTCAGGCGGTTACCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTAAGTG                                <  SRR4302533.1848170/94‑1 (MQ=60)
                                                             GCTTATTGGTTAAA‑TCCTTCAGGCGGGTCCCGCGTCCCCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTAAGTG                                <  SRR4302533.41827/94‑1 (MQ=60)
                                                              TTTATTGGTTAAA‑TCCTTCAGGCGGGTCCCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTAAGTGA                               <  SRR4302533.2466568/94‑1 (MQ=60)
                                                              CTTATTGATTAAA‑TCCTTCAGGCGGGTCCCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTAAGTGA                               <  SRR4302533.1386909/94‑1 (MQ=60)
                                                               TTATTGTTTAAA‑CCCTTCAGGCGGGTCCCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTAAGTGAT                              <  SRR4302533.5553053/94‑1 (MQ=60)
                                                               TTATTGGTTAAA‑TCCTTCAGGCGGGTACCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTAAGTGAT                              >  SRR4302533.5862463/1‑94 (MQ=60)
                                                               TTATTGGTTAAA‑TCCTTCAGGCGGGTACCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTAAGTGAT                              <  SRR4302533.5150689/94‑1 (MQ=60)
                                                                TATTGGTTAAA‑TCCTTCAGGCGGGTCCCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTAAGTGATC                             <  SRR4302533.5937530/94‑1 (MQ=60)
                                                                TATTGGTTAAA‑TCCTTCAGGCGGGTACCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTAAGTGATC                             >  SRR4302533.5414830/1‑94 (MQ=60)
                                                                  tttgtttaaaccctcCAGGCGGGTACCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTAAGTGATCG                            <  SRR4302533.4717858/79‑1 (MQ=60)
                                                                  TGGGTTAAA‑TCCTCGAGGGGGGTACCGCGCCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTAAGTGATCGT                           <  SRR4302533.1075926/94‑1 (MQ=60)
                                                                   TGGTTAAA‑TCCTTCAGGCGGGTCCCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTAAGTGATCGTT                          <  SRR4302533.4583276/94‑1 (MQ=60)
                                                                   TGGTTAAA‑TCCTTCAGGCGGGTACCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTAAGTGATCGTT                          >  SRR4302533.3544909/1‑94 (MQ=60)
                                                                   TGGTTAAA‑CCCTTCGGGCGGGTCCCGCGCCCCCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTAAGTGATCGTT                          <  SRR4302533.356443/94‑1 (MQ=60)
                                                                    GGTTAAA‑TCCTTCAGGCGGGTACCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCACCAACATTAAGTGATCGTTC                         >  SRR4302533.4231287/1‑94 (MQ=60)
                                                                     GTTAAA‑TCCTTCAGGCGGGTACCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTAAGTGATCGTTCT                        >  SRR4302533.2677599/1‑94 (MQ=60)
                                                                     GTTAAA‑TCCTTCAGGCGGGTACCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTAAGTGATCGTTCT                        >  SRR4302533.5934798/1‑94 (MQ=60)
                                                                      TTAAA‑TCCTTCAGGCGGGTCCCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTAAGTGATCGTTCTT                       <  SRR4302533.5583337/94‑1 (MQ=60)
                                                                       TAAACTCCTTCAGGCGGGTACCGCCTCCCCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTAAGTGATCGTTCTT                       <  SRR4302533.1542369/94‑1 (MQ=60)
                                                                       TAAA‑TCCTTCAGGCGGGTACCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTAAGTGATCGTTCTTC                      <  SRR4302533.5115219/94‑1 (MQ=60)
                                                                       TAAA‑TCCTCCAGGCGGGTACCGCGCCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTAAGTGATCGTTCTTC                      <  SRR4302533.3364462/94‑1 (MQ=60)
                                                                        AAA‑TCCTTCAGGGGGGTCCCGCCTCCCCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTAAGTGTTCGTTCTTCT                     <  SRR4302533.3928519/94‑1 (MQ=60)
                                                                        AAA‑TCCTTCAGGCGGGTACCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTAAGTGATCGTTCTTCT                     >  SRR4302533.4633259/1‑94 (MQ=60)
                                                                        AAA‑TCCTTCAGGCGGGTACCGCGTCCCCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTAAGTGATCGTTCTTCT                     <  SRR4302533.5139311/94‑1 (MQ=60)
                                                                          A‑TCCTTCGGGCGGTTCCCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTAAGTGATCGTTCTTCTCT                   <  SRR4302533.2354563/94‑1 (MQ=60)
                                                                          A‑TCCTTCAGGCGGGTACCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTAAGTGATCGTTCTTCTCT                   >  SRR4302533.4407865/1‑94 (MQ=60)
                                                                            TCCTTCAGGCGGGTACCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTAAGTGATCGTTCTTCTCTA                  >  SRR4302533.5732769/1‑94 (MQ=60)
                                                                            TCCTTCAGGCGGGTACCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTAAGTGATCGTTCTTCTCTA                  >  SRR4302533.3067225/1‑94 (MQ=60)
                                                                            TCCTTCAGGCGGGTACCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTAAGTGATCGTTCTTCTCTA                  >  SRR4302533.966746/1‑94 (MQ=60)
                                                                             CCTTGAGGCGGTTCCCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTAAGTGATCGTTCTTCTCTAA                 <  SRR4302533.3810082/94‑1 (MQ=60)
                                                                              CTTCAGGCGGGTACCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTAAGTGATCGTTCTTCTCTAAA                >  SRR4302533.2739721/1‑94 (MQ=60)
                                                                               TTCAGGCGGGTACCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTAAGTGATCGTTCTTCTCTAAAC               >  SRR4302533.4278748/1‑94 (MQ=60)
                                                                                TCAGGCGGGTACCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTAAGTGATCGTTCTTCTCTAAACT              >  SRR4302533.80231/1‑94 (MQ=60)
                                                                                 CAGGCGGGTACCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTAAGTGATCGTTCTTCTCTAAACTA             >  SRR4302533.934838/1‑94 (MQ=60)
                                                                                  AGGCGGGTACCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCCACATTAAGTGATCGTTCTTCTCTAAACTAA            >  SRR4302533.1076795/1‑94 (MQ=60)
                                                                                   GGCGGGTACCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTAAGTGATCGTTCTTCTCTAAACTAAC           <  SRR4302533.1079403/94‑1 (MQ=60)
                                                                                    GCGGGTACCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTAAGTGATCGTTCTTCTCTAAACTAACC          >  SRR4302533.5452842/1‑94 (MQ=60)
                                                                                    GCGGGTACCGCCTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTAAGTGATCGTTCTTCTCTAAACTAACC          <  SRR4302533.2417131/94‑1 (MQ=60)
                                                                                     CGGGTCCCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTAAGTGATCGTTCTTCTCTAAACTAACCA         <  SRR4302533.3655336/94‑1 (MQ=60)
                                                                                     CGGGTACCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCTCGCCAACATTAAGTGATCGTTCTTCTCTAAAATAATCA         >  SRR4302533.236080/1‑94 (MQ=60)
                                                                                     CGGGTACCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTAAGTGATCGTTCTTCTCTAAACTAACCA         <  SRR4302533.1039581/94‑1 (MQ=60)
                                                                                      GGGTACCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTAAGTGATCGTTCTTCTCTAAACTAACCAT        >  SRR4302533.4992515/1‑94 (MQ=60)
                                                                                      GGGTACCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTAAGTGATCGTTCTTCTCTAAACTAACCAT        >  SRR4302533.2735373/1‑94 (MQ=60)
                                                                                          ACCGCGTCCTCCCTCCAGTATCAGGGCAACAGATTTCAATGGCAcctgcaccgccacccttagtgagtcgttcttctctacactacctatgact    >  SRR4302533.4861237/1‑44 (MQ=60)
                                                                                          ACCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTAAGTGATCGTTCTTCTCTAAACTAACCATGACT    >  SRR4302533.4861238/1‑94 (MQ=60)
                                                                                           CCGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTAAGTGATCGTTCTTCTCTAAACTAACCATGACTA   <  SRR4302533.285717/94‑1 (MQ=60)
                                                                                            CGCGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTAAGTGATCGTTCTTCTCTAAACTAACCATGACTAA  <  SRR4302533.6287518/94‑1 (MQ=60)
                                                                                             |                                                                                            
TTAGACAGCGCAAAGTCGATAATGCGGAACTTACCGCCGAAGTGTACGGCCGGTTTTGCTCGCTTATTGGTTAAA‑TCCTTCAGGCGGGTACCACGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTAAGTGATCGTTCTTCTCTAAACTAACCATGACTAA  >  NC_000913/3566783‑3566967

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑