Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,627,142 +G intergenic (‑24/+23) yhiH ← / ← yhiI putative ATP‑binding component of a transport system, fragment 1/putative membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,627,1401.G99.6% 745.9 / ‑4.3 233intergenic (‑22/+25)yhiH/yhiIputative ATP‑binding component of a transport system, fragment 1/putative membrane protein
Reads supporting (aligned to +/- strand):  ref base . (1/0);  new base G (134/98);  total (135/98)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 277 total aligned reads displayed.

ACCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGC‑GGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCA  >  NC_000913/3627048‑3627227
                                                                                             |                                                                                       
aCCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGc                                                                                          <  2:3776689/93‑1 (MQ=255)
 ccATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGC‑g                                                                                        >  2:6083111/1‑93 (MQ=255)
 ccATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGC‑g                                                                                        <  2:1898925/93‑1 (MQ=255)
 ccATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGC‑g                                                                                        <  2:2444146/93‑1 (MQ=255)
 ccATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGC‑g                                                                                        <  1:3467865/93‑1 (MQ=255)
 ccATACAGCGGGCCGGAATATCGAGAGTGATATTGTCCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGC‑g                                                                                        <  2:2714517/93‑1 (MQ=255)
  cATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGC‑gg                                                                                       >  1:987636/1‑93 (MQ=255)
  cATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGATGCGCGAAAGGC‑gg                                                                                       >  2:3186105/1‑93 (MQ=255)
   aTACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGC‑ggg                                                                                      <  1:2214603/93‑2 (MQ=255)
   aTACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGC‑ggg                                                                                      >  1:4342352/1‑92 (MQ=255)
    tACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGC‑ggga                                                                                     >  1:233825/1‑91 (MQ=255)
      cAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGC‑ggggcg                                                                                   >  1:5040681/1‑89 (MQ=255)
      cAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGC‑gggacg                                                                                   <  1:5576349/93‑5 (MQ=255)
      cAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGC‑gggacg                                                                                   <  2:4466478/93‑5 (MQ=255)
        gCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACggg                                                                                  >  2:549831/1‑93 (MQ=255)
        gCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACggg                                                                                  <  2:2949362/93‑1 (MQ=255)
        gCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACggg                                                                                  <  1:3129365/93‑1 (MQ=255)
                  atatCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTcc                                                                        >  1:773058/1‑93 (MQ=255)
                   tatCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCa                                                                       <  1:4914715/93‑1 (MQ=255)
                    atCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAg                                                                      >  1:2788960/1‑93 (MQ=255)
                     tCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGa                                                                     <  1:4064684/93‑1 (MQ=255)
                     tCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGa                                                                     >  1:2607756/1‑93 (MQ=255)
                     tCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGa                                                                     <  1:4498134/93‑1 (MQ=255)
                      cGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGAt                                                                    <  1:6044456/93‑1 (MQ=255)
                      cGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGAt                                                                    <  1:3211936/93‑1 (MQ=255)
                        agagTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGc                                                                  >  2:3892948/1‑93 (MQ=255)
                             gATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCAt                                                             <  2:6252708/93‑1 (MQ=255)
                              atatTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGGACCAGGTCCAGATGCGTCAtt                                                            >  2:1310811/1‑93 (MQ=255)
                                 ttGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCg                                                         >  1:5768559/1‑93 (MQ=255)
                                 ttGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCg                                                         >  2:193529/1‑93 (MQ=255)
                                    ttCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCa                                                      >  1:4979504/1‑93 (MQ=255)
                                     tCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCaa                                                     <  1:2525138/93‑1 (MQ=255)
                                     tCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCaa                                                     >  2:4720263/1‑93 (MQ=255)
                                     tCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCaa                                                     >  2:692706/1‑93 (MQ=255)
                                      cAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACCGGAACCAGTTCCCGATGCGTCATTGCCGCCAc                                                    >  2:2546020/1‑93 (MQ=255)
                                       aGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAAcc                                                   <  1:4622190/93‑1 (MQ=255)
                                       aGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAAcc                                                   >  2:5085269/1‑93 (MQ=255)
                                        gcgcAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCt                                                  >  1:1790195/1‑93 (MQ=255)
                                          gcAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTca                                                >  1:2410825/1‑93 (MQ=255)
                                             aCGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTcacca                                             <  2:4902634/93‑1 (MQ=255)
                                             aCGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTcacca                                             <  2:4548958/93‑1 (MQ=255)
                                              cGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTcaccac                                            >  1:6071581/1‑93 (MQ=255)
                                               ggTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACg                                           >  2:4255137/1‑93 (MQ=255)
                                                gTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGa                                          <  1:2911538/93‑1 (MQ=255)
                                                gTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGa                                          <  2:4017329/93‑1 (MQ=255)
                                                 tttttCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGTCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAg                                         >  1:4449638/1‑93 (MQ=255)
                                                 tttttCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAg                                         <  2:568522/93‑1 (MQ=255)
                                                  ttttCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAgg                                        >  1:3593376/1‑93 (MQ=255)
                                                  ttttCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAgg                                        <  2:1247780/93‑1 (MQ=255)
                                                   tttCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGgt                                       <  1:6180266/93‑1 (MQ=255)
                                                   tttCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGgt                                       >  2:3927336/1‑93 (MQ=255)
                                                    ttCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGgtc                                      >  1:5698503/1‑93 (MQ=255)
                                                     tCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGgtcg                                     >  1:1311231/1‑93 (MQ=255)
                                                      ccATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGgtcgt                                    >  2:1474172/1‑93 (MQ=255)
                                                      ccATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGgtcgt                                    >  1:4000036/1‑93 (MQ=255)
                                                      ccATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCAACACGAGgtcgt                                    >  1:3296101/1‑93 (MQ=255)
                                                       cATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGgtcgtc                                   >  2:2311443/1‑93 (MQ=255)
                                                        aTAATGCTGTCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCa                                  <  2:5841838/93‑1 (MQ=255)
                                                        aTAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCCCCACGAGGTCGTCa                                  <  1:5268781/93‑1 (MQ=255)
                                                        aTAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGgtcgtcc                                  >  2:1600396/1‑92 (MQ=255)
                                                        aTAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCa                                  <  2:4098155/93‑1 (MQ=255)
                                                          aaTGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAgg                                >  1:856873/1‑93 (MQ=255)
                                                           aTGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCCACCTCACCACGAGGTCGTCCGGc                               >  2:3601859/1‑93 (MQ=255)
                                                           aTGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGc                               >  2:3105425/1‑93 (MQ=255)
                                                            tGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGcc                              >  2:1084536/1‑93 (MQ=255)
                                                            tGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGcc                              >  2:3373060/1‑93 (MQ=255)
                                                             gCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCa                             >  1:1446469/1‑93 (MQ=255)
                                                             gCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCa                             >  1:4215803/1‑93 (MQ=255)
                                                              cTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCAc                            >  2:2483410/1‑93 (MQ=255)
                                                              cTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCAc                            <  1:4597381/93‑1 (MQ=255)
                                                               tGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACg                           >  2:1197771/1‑93 (MQ=255)
                                                                ggCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGGACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGGCGTCAGGCCACgg                          >  1:3243067/1‑93 (MQ=255)
                                                                   tCACGCCCGCCAGTTGCGTGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAg                       <  1:1958991/93‑1 (MQ=255)
                                                                   tCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGacg                       >  2:6071009/1‑91 (MQ=255)
                                                                   tCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAg                       >  2:479020/1‑93 (MQ=255)
                                                                   tCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAg                       >  1:2494280/1‑93 (MQ=255)
                                                                   tCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAg                       <  2:1455185/93‑1 (MQ=255)
                                                                   tCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAg                       >  2:5768053/1‑93 (MQ=255)
                                                                    cACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGt                      >  2:5126804/1‑93 (MQ=255)
                                                                     aCGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGtt                     >  2:2780032/1‑93 (MQ=255)
                                                                     aCGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGtt                     >  1:5108768/1‑93 (MQ=255)
                                                                      cgcccgccAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGttc                    <  2:95092/93‑1 (MQ=255)
                                                                          cgccAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCttca                >  2:1765422/1‑93 (MQ=255)
                                                                           gccAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCttcat               >  1:1242273/1‑93 (MQ=255)
                                                                           gccAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCttcat               >  2:5380004/1‑93 (MQ=255)
                                                                            ccAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCttcatt              >  1:1142111/1‑93 (MQ=255)
                                                                            ccAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCttcatt              <  2:2800193/93‑1 (MQ=255)
                                                                            ccAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCttcatt              >  1:5977336/1‑93 (MQ=255)
                                                                             cAGTTGCGCGACAGGCGGGCCGGGAACCAGTTCCCGATGCGTCATTGGGGCAACCTCACCACGAGGACGTCAGGGCACGGGAGATattcattc             >  2:524946/1‑93 (MQ=255)
                                                                             cAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCttcattc             >  2:3738102/1‑93 (MQ=255)
                                                                             cAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCttcattc             >  1:2805195/1‑93 (MQ=255)
                                                                             cAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCttcattc             >  2:2605625/1‑93 (MQ=255)
                                                                              aGTTGCGCGACAGTCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCttcattca            >  1:3384066/1‑93 (MQ=255)
                                                                              aGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCttcattca            <  1:3720829/93‑1 (MQ=255)
                                                                              aGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCttcattca            <  2:4170693/93‑1 (MQ=255)
                                                                                 tGCGCGAGAGGCGGGACGGGAACCAGTGCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCAccc         <  2:561944/93‑1 (MQ=255)
                                                                                 tGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCAccc         <  2:2073291/93‑1 (MQ=255)
                                                                                      gACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCAcc    <  1:5413083/93‑1 (MQ=255)
                                                                                      gACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCAcc    >  2:50134/1‑93 (MQ=255)
                                                                                       aCAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCAccc   >  1:2232115/1‑93 (MQ=255)
                                                                                             |                                                                                       
ACCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGC‑GGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCA  >  NC_000913/3627048‑3627227

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGC‑GGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCACGCTACG  >  NC_000913/3627049‑3627234
                                                                                              |                                                                                            
CCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGC‑GG                                                                                              >  SRR4302533.993415/1‑94 (MQ=60)
CCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGC‑GG                                                                                              <  SRR4302533.3489271/94‑1 (MQ=60)
CCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGGTGCGCGACAGGC‑GG                                                                                              >  SRR4302533.4779441/1‑94 (MQ=60)
 CATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGC‑GGG                                                                                             <  SRR4302533.4477138/94‑1 (MQ=60)
 CATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGC‑GGG                                                                                             >  SRR4302533.4368521/1‑94 (MQ=60)
 CATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGC‑GGG                                                                                             <  SRR4302533.5598200/94‑1 (MQ=60)
 CATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGC‑GGG                                                                                             >  SRR4302533.4653063/1‑94 (MQ=60)
 CATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGC‑GGG                                                                                             <  SRR4302533.5986664/94‑1 (MQ=60)
  ATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGC‑GGga                                                                                            >  SRR4302533.4635115/1‑92 (MQ=60)
  ATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGC‑GGga                                                                                            >  SRR4302533.235268/1‑92 (MQ=60)
  ATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGC‑GGga                                                                                            <  SRR4302533.2228269/94‑3 (MQ=60)
   TACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGC‑GGgac                                                                                           <  SRR4302533.4532947/94‑4 (MQ=60)
    ACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGC‑GGggcg                                                                                          >  SRR4302533.5071192/1‑90 (MQ=60)
     CAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGG                                                                                          <  SRR4302533.5610051/94‑1 (MQ=60)
     CAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGG                                                                                          <  SRR4302533.5312438/94‑1 (MQ=60)
     CAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGG                                                                                          >  SRR4302533.2425777/1‑94 (MQ=60)
     CAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCGGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGG                                                                                          <  SRR4302533.3571553/94‑1 (MQ=60)
      AGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGC‑GGgaccgg                                                                                        >  SRR4302533.3687267/1‑88 (MQ=60)
      AGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGC‑GGgaaggg                                                                                        >  SRR4302533.3819677/1‑88 (MQ=60)
       GCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGA                                                                                        <  SRR4302533.2617631/94‑1 (MQ=60)
       GCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGA                                                                                        <  SRR4302533.3148606/94‑1 (MQ=60)
         GGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAAC                                                                                      >  SRR4302533.1713177/1‑94 (MQ=60)
         GAGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAAC                                                                                      <  SRR4302533.5330008/94‑1 (MQ=60)
            CCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAG                                                                                   >  SRR4302533.3119306/1‑94 (MQ=60)
              GGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTT                                                                                 <  SRR4302533.889520/94‑1 (MQ=60)
                AATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCC                                                                               >  SRR4302533.777603/1‑94 (MQ=60)
                 ATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCA                                                                              >  SRR4302533.582086/1‑94 (MQ=60)
                  TATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAG                                                                             <  SRR4302533.4944485/94‑1 (MQ=60)
                  TATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAG                                                                             >  SRR4302533.2806103/1‑94 (MQ=60)
                  TATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAG                                                                             <  SRR4302533.2915697/94‑1 (MQ=60)
                  TATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAG                                                                             >  SRR4302533.3216569/1‑94 (MQ=60)
                  TATCGAGAGTGATATTATTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAG                                                                             <  SRR4302533.3391094/94‑1 (MQ=60)
                   ATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGA                                                                            >  SRR4302533.2623770/1‑94 (MQ=60)
                   ATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGA                                                                            >  SRR4302533.3355226/1‑94 (MQ=60)
                    TCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGAT                                                                           <  SRR4302533.4525375/94‑1 (MQ=60)
                    TCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGAT                                                                           <  SRR4302533.4089425/94‑1 (MQ=60)
                    TCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGAT                                                                           <  SRR4302533.2808949/94‑1 (MQ=60)
                    TCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGAT                                                                           <  SRR4302533.4197154/94‑1 (MQ=60)
                    TCGAGAGTGATATTGTCCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGAT                                                                           <  SRR4302533.6181534/94‑1 (MQ=60)
                     CGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATG                                                                          <  SRR4302533.3231720/94‑1 (MQ=60)
                     CGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATG                                                                          <  SRR4302533.6081074/94‑1 (MQ=60)
                      GAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGC                                                                         >  SRR4302533.216141/1‑94 (MQ=60)
                       AGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGGACCAGTTCCAGAAGCG                                                                        >  SRR4302533.2418359/1‑94 (MQ=60)
                       AGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAAGCAGCTCCAGATGCG                                                                        >  SRR4302533.4993161/1‑94 (MQ=60)
                       AGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCG                                                                        <  SRR4302533.4280563/94‑1 (MQ=60)
                          GTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCA                                                                     <  SRR4302533.5156902/94‑1 (MQ=60)
                          GTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCA                                                                     >  SRR4302533.3765863/1‑94 (MQ=60)
                           TGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGGTCCAGATGCGTCAT                                                                    >  SRR4302533.665456/1‑94 (MQ=60)
                            TATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATT                                                                   <  SRR4302533.3237566/94‑1 (MQ=60)
                            GANATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCCACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATT                                                                   <  SRR4302533.3702527/94‑1 (MQ=60)
                             ATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTG                                                                  <  SRR4302533.3286833/94‑1 (MQ=60)
                             ATATTGTGCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTG                                                                  <  SRR4302533.2832340/94‑1 (MQ=60)
                              TATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGC                                                                 >  SRR4302533.2789959/1‑94 (MQ=60)
                              TATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGC                                                                 >  SRR4302533.3183311/1‑94 (MQ=60)
                              TATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGC                                                                 <  SRR4302533.2312168/94‑1 (MQ=60)
                               ATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGCACCAGTTCCAGATGCGTCATTGCG                                                                >  SRR4302533.3860075/1‑94 (MQ=60)
                               ATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCG                                                                >  SRR4302533.5803382/1‑94 (MQ=60)
                               ATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCG                                                                <  SRR4302533.4027410/94‑1 (MQ=60)
                               ATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCG                                                                <  SRR4302533.4197444/94‑1 (MQ=60)
                                  GTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCA                                                             >  SRR4302533.845972/1‑94 (MQ=60)
                                  GTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCA                                                             >  SRR4302533.4428679/1‑94 (MQ=60)
                                  GTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCA                                                             >  SRR4302533.5009697/1‑94 (MQ=60)
                                   TTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAA                                                            <  SRR4302533.1209883/94‑1 (MQ=60)
                                    TCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAAC                                                           <  SRR4302533.2540688/94‑1 (MQ=60)
                                     CAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAGCCAGTTCCAGATGCGTCATTtcgggaccc                                                          >  SRR4302533.1306603/1‑85 (MQ=60)
                                      AGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGtcgcgagcc                                                         >  SRR4302533.1515262/1‑85 (MQ=60)
                                      AGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCT                                                         <  SRR4302533.4650203/94‑1 (MQ=60)
                                      AGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCT                                                         >  SRR4302533.1801083/1‑94 (MQ=60)
                                        CGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCA                                                       <  SRR4302533.3098341/94‑1 (MQ=60)
                                        CGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCA                                                       >  SRR4302533.2425716/1‑94 (MQ=60)
                                          CAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACC                                                     <  SRR4302533.4083481/94‑1 (MQ=60)
                                            ACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCAC                                                   <  SRR4302533.5024432/94‑1 (MQ=60)
                                            ACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCAC                                                   >  SRR4302533.6108313/1‑94 (MQ=60)
                                             CGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACG                                                  <  SRR4302533.4497548/94‑1 (MQ=60)
                                              GGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGCCATTGCGGCAACCTCACCACGA                                                 <  SRR4302533.6289650/94‑1 (MQ=60)
                                               GTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGTCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAG                                                >  SRR4302533.4476547/1‑94 (MQ=60)
                                               GTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAG                                                <  SRR4302533.2280512/94‑1 (MQ=60)
                                               GTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAG                                                <  SRR4302533.2929409/94‑1 (MQ=60)
                                                TTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGG                                               >  SRR4302533.3615488/1‑94 (MQ=60)
                                                 TTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGT                                              <  SRR4302533.3477067/94‑1 (MQ=60)
                                                  TTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTC                                             <  SRR4302533.6085341/94‑1 (MQ=60)
                                                  TTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTC                                             >  SRR4302533.5732921/1‑94 (MQ=60)
                                                  TTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTC                                             <  SRR4302533.6217381/94‑1 (MQ=60)
                                                  TTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTC                                             <  SRR4302533.140557/94‑1 (MQ=60)
                                                   TTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCG                                            >  SRR4302533.1319103/1‑94 (MQ=60)
                                                    TCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGT                                           >  SRR4302533.4024393/1‑94 (MQ=60)
                                                    TCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCAACACGAGGTCGT                                           >  SRR4302533.3316446/1‑94 (MQ=60)
                                                     CCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTC                                          >  SRR4302533.5672310/1‑94 (MQ=60)
                                                      CATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCA                                         <  SRR4302533.1297090/94‑1 (MQ=60)
                                                      CATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCA                                         <  SRR4302533.57511/94‑1 (MQ=60)
                                                      CATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGACGTCA                                         >  SRR4302533.3663014/1‑94 (MQ=60)
                                                       ATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCCCCACGAGGTCGTCAG                                        <  SRR4302533.5300386/94‑1 (MQ=60)
                                                       ATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAG                                        >  SRR4302533.4992711/1‑94 (MQ=60)
                                                        TAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGG                                       >  SRR4302533.861892/1‑94 (MQ=60)
                                                          ATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCC                                     <  SRR4302533.2496025/94‑1 (MQ=60)
                                                           TGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCA                                    >  SRR4302533.1455195/1‑94 (MQ=60)
                                                           TGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCA                                    >  SRR4302533.4241306/1‑94 (MQ=60)
                                                            GCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCAC                                   >  SRR4302533.438052/1‑94 (MQ=60)
                                                             CTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACG                                  <  SRR4302533.4625230/94‑1 (MQ=60)
                                                             CTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACG                                  >  SRR4302533.2531269/1‑94 (MQ=60)
                                                             CTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACG                                  >  SRR4302533.1389496/1‑94 (MQ=60)
                                                              TGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGGACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGGCGTCAGGCCACGG                                 >  SRR4302533.3263053/1‑94 (MQ=60)
                                                              TGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGG                                 >  SRR4302533.5228533/1‑94 (MQ=60)
                                                               GGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGA                                >  SRR4302533.6054995/1‑94 (MQ=60)
                                                               GGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGA                                <  SRR4302533.3284362/94‑1 (MQ=60)
                                                                GCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAA                               >  SRR4302533.553366/1‑94 (MQ=60)
                                                                 CTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAG                              >  SRR4302533.6073669/1‑94 (MQ=60)
                                                                 CTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAG                              >  SRR4302533.2509667/1‑94 (MQ=60)
                                                                  TCACGCCCGCCAGTTGCGTGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGT                             <  SRR4302533.1970948/94‑1 (MQ=60)
                                                                   CACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTT                            >  SRR4302533.5139638/1‑94 (MQ=60)
                                                                   CACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTT                            <  SRR4302533.2535959/94‑1 (MQ=60)
                                                                      GCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTT                         >  SRR4302533.3478784/1‑94 (MQ=60)
                                                                         CGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCAT                      >  SRR4302533.2242683/1‑94 (MQ=60)
                                                                         CGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCAT                      >  SRR4302533.1249693/1‑94 (MQ=60)
                                                                          GCCAGTTGCGCGACAGGCGGGACGGGGACCAGTTCCAGATGCGTCATTGCGGCAAACTCACCACGAGGGCGTCAGGCCACGGAAGGTCTTCATT                     >  SRR4302533.3319329/1‑94 (MQ=60)
                                                                          GCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATT                     >  SRR4302533.2238090/1‑94 (MQ=60)
                                                                          GCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATT                     >  SRR4302533.6013540/1‑94 (MQ=60)
                                                                          GCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATT                     >  SRR4302533.2493637/1‑94 (MQ=60)
                                                                          GCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATT                     >  SRR4302533.783445/1‑94 (MQ=60)
                                                                          GCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATT                     >  SRR4302533.1148919/1‑94 (MQ=60)
                                                                           CCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTC                    >  SRR4302533.2822461/1‑94 (MQ=60)
                                                                            CAGTTGCGCGACAGTCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCA                   >  SRR4302533.3404960/1‑94 (MQ=60)
                                                                            CAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCA                   <  SRR4302533.512571/94‑1 (MQ=60)
                                                                            CAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCA                   <  SRR4302533.5049942/94‑1 (MQ=60)
                                                                             AGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCAC                  <  SRR4302533.5635922/94‑1 (MQ=60)
                                                                             AGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCAC                  <  SRR4302533.3743663/94‑1 (MQ=60)
                                                                             AGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCAC                  >  SRR4302533.1275251/1‑94 (MQ=60)
                                                                             AGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGACGTCAGGCCACGGAAGTTCTTCATTCTC                  >  SRR4302533.6096130/1‑94 (MQ=60)
                                                                              GTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACC                 <  SRR4302533.2682868/94‑1 (MQ=60)
                                                                                TGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCG               <  SRR4302533.1799196/94‑1 (MQ=60)
                                                                                  CGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCC             >  SRR4302533.1926057/1‑94 (MQ=60)
                                                                                    CGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTGACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACC           >  SRR4302533.796942/1‑94 (MQ=60)
                                                                                     GACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCC          >  SRR4302533.2245886/1‑94 (MQ=60)
                                                                                     GACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCC          >  SRR4302533.939971/1‑94 (MQ=60)
                                                                                     GACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCC          <  SRR4302533.5445632/94‑1 (MQ=60)
                                                                                      ACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCA         >  SRR4302533.2550817/1‑94 (MQ=60)
                                                                                       CAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCAC        <  SRR4302533.4605313/94‑1 (MQ=60)
                                                                                        AGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCACG       <  SRR4302533.3188007/94‑1 (MQ=60)
                                                                                         GGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCACGC      <  SRR4302533.4046949/94‑1 (MQ=60)
                                                                                          gcgGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCACGCT     <  SRR4302533.5084605/91‑1 (MQ=60)
                                                                                          gcgGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCACGCT     <  SRR4302533.1924414/91‑1 (MQ=60)
                                                                                           cgGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCACGCTA    <  SRR4302533.4772903/92‑1 (MQ=60)
                                                                                           cgGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCACGCTA    <  SRR4302533.6064231/92‑1 (MQ=60)
                                                                                           G‑GGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCACGCTAC   <  SRR4302533.3775297/94‑1 (MQ=60)
                                                                                             GGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCACGCTACG  <  SRR4302533.4488167/94‑1 (MQ=60)
                                                                                             GGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCACGCTACG  <  SRR4302533.3983535/94‑1 (MQ=60)
                                                                                             GGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCACGCTACG  <  SRR4302533.4273195/94‑1 (MQ=60)
                                                                                             GGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGATCTTCATTCACCCGCACCCACGCTACG  >  SRR4302533.558592/1‑94 (MQ=60)
                                                                                              |                                                                                            
CCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGC‑GGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCACGCTACG  >  NC_000913/3627049‑3627234

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑