Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,731,386	T→C	V13A (GTA→GCA)	xylH →	XylH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,731,386	0	T	C	100.0%	838.3 / NA	260	V13A (GTA→GCA)	xylH	XylH
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (123/137); total (123/137)

Only 100 of 269 total aligned reads displayed.

TCATAACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGTACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCTGTTCT  >  NC_000913/3731296‑3731475

tCATAACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCAc                                                                                         >  1:6171444/1‑93 (MQ=255)

 cATAACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCAcc                                                                                        <  2:5658910/93‑1 (MQ=255)

  aTAACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCg                                                                                       >  1:347868/1‑93 (MQ=255)

  aTAACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCg                                                                                       >  2:5136710/1‑93 (MQ=255)

  aTAACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCg                                                                                       <  2:4924398/93‑1 (MQ=255)

    aaCCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGAc                                                                                     >  1:132928/1‑93 (MQ=255)

    aaCCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAAATGGCCGCACCGAc                                                                                     >  1:212041/1‑93 (MQ=255)

      ccTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACAt                                                                                   >  2:3268906/1‑93 (MQ=255)

       cTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATc                                                                                  <  1:1667407/93‑1 (MQ=255)

       cTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATc                                                                                  >  2:3329602/1‑93 (MQ=255)

       cTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATc                                                                                  <  2:5838182/93‑1 (MQ=255)

        tGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATcc                                                                                 >  1:1485732/1‑93 (MQ=255)

         gACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCg                                                                                >  2:4771226/1‑93 (MQ=255)

          aCTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCgg                                                                               <  1:1953284/93‑1 (MQ=255)

           cTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCTCACCGACATCCGGt                                                                              <  1:2650907/93‑1 (MQ=255)

            tCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTg                                                                             <  1:2856326/93‑1 (MQ=255)

            tCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTg                                                                             >  1:428690/1‑93 (MQ=255)

             cAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGGGAAATTGGCCGCACCGACATCgggggg                                                                            >  1:4428600/1‑93 (MQ=255)

               ggAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCt                                                                          >  2:1620556/1‑93 (MQ=255)

                gAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCtt                                                                         <  1:6244455/93‑1 (MQ=255)

                 aGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTtc                                                                        <  2:4838495/93‑1 (MQ=255)

                 aGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTtc                                                                        <  1:6113341/93‑1 (MQ=255)

                  gCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTtct                                                                       <  2:4453245/93‑1 (MQ=255)

                   cAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTtctc                                                                      <  1:1027446/93‑1 (MQ=255)

                    aGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTcc                                                                     >  2:2303041/1‑93 (MQ=255)

                    aGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTcc                                                                     <  2:2795232/93‑1 (MQ=255)

                     ggTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCg                                                                    <  1:4218607/93‑1 (MQ=255)

                      gTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCgg                                                                   <  1:1497148/93‑1 (MQ=255)

                      gTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCgg                                                                   >  1:2008309/1‑93 (MQ=255)

                      gTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCgg                                                                   >  2:2573347/1‑93 (MQ=255)

                        gATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGc                                                                 >  2:2836838/1‑93 (MQ=255)

                         aTGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCt                                                                >  2:6274682/1‑93 (MQ=255)

                         aTGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCt                                                                <  1:2404210/93‑1 (MQ=255)

                           ggAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGa                                                              <  1:2469434/93‑1 (MQ=255)

                           ggAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGa                                                              <  1:5037654/93‑1 (MQ=255)

                           ggAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGa                                                              >  2:4573015/1‑93 (MQ=255)

                            gAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGaa                                                             >  1:3247139/1‑93 (MQ=255)

                              aGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAAt                                                           <  1:4807026/93‑1 (MQ=255)

                               gCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATc                                                          <  2:3717852/93‑1 (MQ=255)

                               gCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATc                                                          <  1:5960609/93‑1 (MQ=255)

                                 cGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCAc                                                        >  1:2458308/1‑93 (MQ=255)

                                   cATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTg                                                      <  2:1142015/93‑1 (MQ=255)

                                      tGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAAt                                                   >  2:3835673/1‑93 (MQ=255)

                                        aggagCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAAttt                                                 >  2:695700/1‑93 (MQ=255)

                                         ggagCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTg                                                >  1:3560924/1‑93 (MQ=255)

                                         ggagCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTg                                                >  1:746745/1‑93 (MQ=255)

                                          gagCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGc                                               <  1:1392826/93‑1 (MQ=255)

                                           agCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCa                                              <  1:23015/93‑1 (MQ=255)

                                            gCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAg                                             >  1:4056818/1‑93 (MQ=255)

                                            gCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAg                                             >  2:1947424/1‑93 (MQ=255)

                                              gAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGt                                           >  2:2482644/1‑93 (MQ=255)

                                              gAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGt                                           >  1:5578021/1‑93 (MQ=255)

                                              gAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGt                                           <  1:6279522/93‑1 (MQ=255)

                                               aaCATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTc                                          >  2:1573225/1‑93 (MQ=255)

                                               aaCATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTc                                          >  1:1143225/1‑93 (MQ=255)

                                               aaCATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTc                                          >  2:490749/1‑93 (MQ=255)

                                                aCATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCt                                         >  2:3898970/1‑93 (MQ=255)

                                                  atcatGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTc                                       <  1:1843105/93‑1 (MQ=255)

                                                  atcatGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTc                                       <  2:6036659/93‑1 (MQ=255)

                                                     atGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGtg                                    >  1:6027050/1‑93 (MQ=255)

                                                      tGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGtga                                   <  1:5471261/93‑1 (MQ=255)

                                                      tGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGtga                                   <  1:2993321/93‑1 (MQ=255)

                                                          gAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTgatgat                               <  2:808428/93‑1 (MQ=255)

                                                          gAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTgatgat                               <  2:1527256/93‑1 (MQ=255)

                                                           aaaaGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGAtt                              >  1:1196736/1‑93 (MQ=255)

                                                            aaaGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTg                             >  1:5290820/1‑93 (MQ=255)

                                                             aaGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGc                            <  2:5907334/93‑1 (MQ=255)

                                                             aaGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGc                            <  2:3350985/93‑1 (MQ=255)

                                                               gCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAg                          <  1:2776348/93‑1 (MQ=255)

                                                               gCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAg                          >  2:2206420/1‑93 (MQ=255)

                                                                 aaTCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCt                        >  1:3653761/1‑93 (MQ=255)

                                                                  aTCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTa                       >  1:2590453/1‑93 (MQ=255)

                                                                  aTCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTa                       >  1:448876/1‑93 (MQ=255)

                                                                   tCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTat                      <  1:5920164/93‑1 (MQ=255)

                                                                   tCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTat                      >  2:538818/1‑93 (MQ=255)

                                                                   tCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTat                      >  2:3576146/1‑93 (MQ=255)

                                                                    ccGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTatc                     <  2:5268100/93‑1 (MQ=255)

                                                                      gTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTatcat                   >  2:3449883/1‑93 (MQ=255)

                                                                      gTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTatcat                   <  2:4110389/93‑1 (MQ=255)

                                                                      gTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTatcat                   <  1:832601/93‑1 (MQ=255)

                                                                       tCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTatcatc                  >  1:5384835/1‑93 (MQ=255)

                                                                       tCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTatcatc                  >  2:1380739/1‑93 (MQ=255)

                                                                         tGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGc                >  1:3933285/1‑93 (MQ=255)

                                                                            aGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAAt             <  1:523658/93‑1 (MQ=255)

                                                                             gTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATc            >  2:2939740/1‑93 (MQ=255)

                                                                              tGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCa           <  1:4128849/93‑1 (MQ=255)

                                                                              tGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCa           <  2:4259803/93‑1 (MQ=255)

                                                                              tGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCa           >  1:4900771/1‑93 (MQ=255)

                                                                              tGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCa           <  1:2075407/93‑1 (MQ=255)

                                                                                aaaTTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATg         >  1:4584994/1‑93 (MQ=255)

                                                                                aaaTTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATg         <  1:2660664/93‑1 (MQ=255)

                                                                                 aaTTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGc        <  2:6255235/93‑1 (MQ=255)

                                                                                 aaTTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGc        >  2:2013150/1‑93 (MQ=255)

                                                                                 aaTTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGc        >  2:2655328/1‑93 (MQ=255)

                                                                                  aTTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCt       >  2:185001/1‑93 (MQ=255)

                                                                                   ttGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCTg      <  1:4617152/93‑1 (MQ=255)

                                                                                    tGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCTGt     >  2:4734221/1‑93 (MQ=255)

                                                                                     ggCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCTGtt    >  2:273730/1‑93 (MQ=255)

                                                                                      gCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCTGTTc   <  1:1045250/93‑1 (MQ=255)

                                                                                       ccGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCTGTTCt  <  2:1012610/93‑1 (MQ=255)

TCATAACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGTACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCTGTTCT  >  NC_000913/3731296‑3731475

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

AAATCATAACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGTACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCTGTTCTTTAC  >  NC_000913/3731293‑3731479

AAATCATAACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGC                                                                                               >  SRR4302533.815473/1‑94 (MQ=60)

AAATCATAACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGC                                                                                               <  SRR4302533.2282010/94‑1 (MQ=60)

 AATCATAACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCA                                                                                              >  SRR4302533.5978005/1‑94 (MQ=60)

  ATCATAACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCAC                                                                                             >  SRR4302533.6208532/1‑94 (MQ=60)

   TCATAACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACC                                                                                            >  SRR4302533.329567/1‑94 (MQ=60)

    CATAACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCG                                                                                           >  SRR4302533.349989/1‑94 (MQ=60)

    CATAACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCG                                                                                           >  SRR4302533.2168672/1‑94 (MQ=60)

      TAACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGAC                                                                                         <  SRR4302533.5280016/94‑1 (MQ=60)

      TAACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGAC                                                                                         <  SRR4302533.6304090/94‑1 (MQ=60)

      TAACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGAC                                                                                         >  SRR4302533.133764/1‑94 (MQ=60)

      TAACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAAATGGCCGCACCGAC                                                                                         >  SRR4302533.213377/1‑94 (MQ=60)

        ACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACAT                                                                                       <  SRR4302533.4051330/94‑1 (MQ=60)

         CCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATC                                                                                      <  SRR4302533.2076790/94‑1 (MQ=60)

         CCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATC                                                                                      >  SRR4302533.6151908/1‑94 (MQ=60)

          CTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCC                                                                                     >  SRR4302533.350260/1‑94 (MQ=60)

          CTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCC                                                                                     >  SRR4302533.3048751/1‑94 (MQ=60)

          CTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCC                                                                                     >  SRR4302533.708351/1‑94 (MQ=60)

          CTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCC                                                                                     >  SRR4302533.1494699/1‑94 (MQ=60)

          CTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCC                                                                                     >  SRR4302533.2819821/1‑94 (MQ=60)

          CTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCC                                                                                     >  SRR4302533.5512304/1‑94 (MQ=60)

          CTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCC                                                                                     >  SRR4302533.548088/1‑94 (MQ=60)

          CTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCC                                                                                     <  SRR4302533.1677480/94‑1 (MQ=60)

           TGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCG                                                                                    <  SRR4302533.1045847/94‑1 (MQ=60)

             ACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGT                                                                                  <  SRR4302533.1965188/94‑1 (MQ=60)

              CTCAGGAGCAGGTGATGGAAGCCGCATTTAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTG                                                                                 >  SRR4302533.4345975/1‑94 (MQ=60)

              CTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCTCACCGACATCCGGTG                                                                                 <  SRR4302533.2667190/94‑1 (MQ=60)

              CTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTG                                                                                 >  SRR4302533.1993528/1‑94 (MQ=60)

              CTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTG                                                                                 >  SRR4302533.431246/1‑94 (MQ=60)

               TCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGG                                                                                <  SRR4302533.2873901/94‑1 (MQ=60)

               TCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGGGAAATTGGCCGCACCGACATCGGGGGG                                                                                >  SRR4302533.4455372/1‑94 (MQ=60)

                 AGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGATCATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACAACCGGTGGCT                                                                              <  SRR4302533.5615929/94‑1 (MQ=60)

                  GGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTT                                                                             >  SRR4302533.2755355/1‑94 (MQ=60)

                   GAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTC                                                                            <  SRR4302533.6281832/94‑1 (MQ=60)

                    AGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCT                                                                           <  SRR4302533.6150234/94‑1 (MQ=60)

                    AGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCT                                                                           >  SRR4302533.3598874/1‑94 (MQ=60)

                     GCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTC                                                                          <  SRR4302533.6314955/94‑1 (MQ=60)

                      CAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCC                                                                         <  SRR4302533.2030823/94‑1 (MQ=60)

                      CAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCC                                                                         <  SRR4302533.1033479/94‑1 (MQ=60)

                       AGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCG                                                                        <  SRR4302533.4950357/94‑1 (MQ=60)

                       AGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCG                                                                        <  SRR4302533.1357243/94‑1 (MQ=60)

                       AGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCG                                                                        >  SRR4302533.2410396/1‑94 (MQ=60)

                        GGTGATTGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGG                                                                       <  SRR4302533.2676503/94‑1 (MQ=60)

                        GGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGG                                                                       >  SRR4302533.5987987/1‑94 (MQ=60)

                        GGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGG                                                                       >  SRR4302533.2020679/1‑94 (MQ=60)

                        GGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGG                                                                       <  SRR4302533.4244122/94‑1 (MQ=60)

                         GTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGG                                                                      <  SRR4302533.5763333/94‑1 (MQ=60)

                         GTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGG                                                                      <  SRR4302533.6219468/94‑1 (MQ=60)

                         GTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGG                                                                      <  SRR4302533.971042/94‑1 (MQ=60)

                         GTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGG                                                                      <  SRR4302533.1506164/94‑1 (MQ=60)

                          TGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGC                                                                     >  SRR4302533.2927524/1‑94 (MQ=60)

                           GATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCT                                                                    <  SRR4302533.3646488/94‑1 (MQ=60)

                           GATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCT                                                                    <  SRR4302533.223366/94‑1 (MQ=60)

                            ATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTG                                                                   >  SRR4302533.4170804/1‑94 (MQ=60)

                            ATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTG                                                                   <  SRR4302533.2419057/94‑1 (MQ=60)

                              GGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAA                                                                 <  SRR4302533.5068159/94‑1 (MQ=60)

                              GGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAA                                                                 >  SRR4302533.3267164/1‑94 (MQ=60)

                              GGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAA                                                                 <  SRR4302533.2484666/94‑1 (MQ=60)

                               GAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAA                                                                <  SRR4302533.4865488/94‑1 (MQ=60)

                               GAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAA                                                                >  SRR4302533.2476134/1‑94 (MQ=60)

                                 AGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATC                                                              <  SRR4302533.4836132/94‑1 (MQ=60)

                                  GCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCA                                                             >  SRR4302533.1499171/1‑94 (MQ=60)

                                  GCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCA                                                             <  SRR4302533.5996669/94‑1 (MQ=60)

                                   CCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCAC                                                            >  SRR4302533.2596037/1‑94 (MQ=60)

                                   CCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCAC                                                            >  SRR4302533.4636127/1‑94 (MQ=60)

                                   CCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCAC                                                            >  SRR4302533.2473476/1‑94 (MQ=60)

                                     GCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTG                                                          <  SRR4302533.4151148/94‑1 (MQ=60)

                                      CATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGA                                                         >  SRR4302533.4772309/1‑94 (MQ=60)

                                        TTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCCGTGGCTTCTCCGGGCTGAAATCACTGAAT                                                       >  SRR4302533.1061635/1‑94 (MQ=60)

                                         TGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATT                                                      <  SRR4302533.5294514/94‑1 (MQ=60)

                                           AGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTG                                                    >  SRR4302533.751141/1‑94 (MQ=60)

                                           AGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTG                                                    >  SRR4302533.3582862/1‑94 (MQ=60)

                                             GAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCA                                                  <  SRR4302533.1401219/94‑1 (MQ=60)

                                              AGCGAAGATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAG                                                 <  SRR4302533.4733220/94‑1 (MQ=60)

                                              AGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAG                                                 <  SRR4302533.23169/94‑1 (MQ=60)

                                              AGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAG                                                 >  SRR4302533.4081514/1‑94 (MQ=60)

                                               GCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGG                                                <  SRR4302533.4610988/94‑1 (MQ=60)

                                                CGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGT                                               >  SRR4302533.5611733/1‑94 (MQ=60)

                                                 GAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTC                                              <  SRR4302533.6317008/94‑1 (MQ=60)

                                                 GAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTC                                              >  SRR4302533.4595355/1‑94 (MQ=60)

                                                 GAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTC                                              >  SRR4302533.1150044/1‑94 (MQ=60)

                                                 GAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTC                                              >  SRR4302533.2316651/1‑94 (MQ=60)

                                                  AACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCT                                             >  SRR4302533.5421144/1‑94 (MQ=60)

                                                  AACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCT                                             <  SRR4302533.1885767/94‑1 (MQ=60)

                                                  AACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCT                                             <  SRR4302533.5584912/94‑1 (MQ=60)

                                                  AACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACACCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCT                                             <  SRR4302533.249588/94‑1 (MQ=60)

                                                    CATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTC                                           <  SRR4302533.4262068/94‑1 (MQ=60)

                                                    CATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCACGTCTTC                                           >  SRR4302533.2452097/1‑94 (MQ=60)

                                                     ATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCG                                          <  SRR4302533.1984058/94‑1 (MQ=60)

                                                     ATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCG                                          <  SRR4302533.1854346/94‑1 (MQ=60)

                                                      TCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGT                                         <  SRR4302533.836920/94‑1 (MQ=60)

                                                      TCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGT                                         <  SRR4302533.271255/94‑1 (MQ=60)

                                                       CATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTG                                        >  SRR4302533.6063573/1‑94 (MQ=60)

                                                       CATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTG                                        >  SRR4302533.4066480/1‑94 (MQ=60)

                                                         TGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGAT                                      <  SRR4302533.3011623/94‑1 (MQ=60)

                                                         TGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGAT                                      >  SRR4302533.1454549/1‑94 (MQ=60)

                                                         TGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGAT                                      <  SRR4302533.5504233/94‑1 (MQ=60)

                                                         TGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGAT                                      <  SRR4302533.1298654/94‑1 (MQ=60)

                                                         TGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGAT                                      >  SRR4302533.2836223/1‑94 (MQ=60)

                                                          GTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATG                                     <  SRR4302533.2663315/94‑1 (MQ=60)

                                                          GTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATG                                     <  SRR4302533.6012013/94‑1 (MQ=60)

                                                             GAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATT                                  <  SRR4302533.6127692/94‑1 (MQ=60)

                                                             GAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATT                                  <  SRR4302533.1765803/94‑1 (MQ=60)

                                                             GAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATT                                  >  SRR4302533.1203926/1‑94 (MQ=60)

                                                              AAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTG                                 >  SRR4302533.4288587/1‑94 (MQ=60)

                                                              AAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTG                                 >  SRR4302533.5322501/1‑94 (MQ=60)

                                                               AAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGC                                <  SRR4302533.1194745/94‑1 (MQ=60)

                                                                AAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCA                               <  SRR4302533.3432828/94‑1 (MQ=60)

                                                                 AGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAG                              <  SRR4302533.4171290/94‑1 (MQ=60)

                                                                  GCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGC                             <  SRR4302533.2793403/94‑1 (MQ=60)

                                                                   CAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCT                            >  SRR4302533.3676226/1‑94 (MQ=60)

                                                                    AATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTA                           >  SRR4302533.2606372/1‑94 (MQ=60)

                                                                    AATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTA                           <  SRR4302533.6276276/94‑1 (MQ=60)

                                                                    AATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTA                           <  SRR4302533.871371/94‑1 (MQ=60)

                                                                    AATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTA                           >  SRR4302533.451560/1‑94 (MQ=60)

                                                                     ATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTAT                          >  SRR4302533.4859322/1‑94 (MQ=60)

                                                                     ATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCAGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTAT                          <  SRR4302533.814068/94‑1 (MQ=60)

                                                                      TCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATC                         <  SRR4302533.5955948/94‑1 (MQ=60)

                                                                       CCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCA                        >  SRR4302533.1486679/1‑94 (MQ=60)

                                                                        CGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCAT                       >  SRR4302533.3711765/1‑94 (MQ=60)

                                                                        CGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCAT                       >  SRR4302533.4486466/1‑94 (MQ=60)

                                                                         GTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATC                      >  SRR4302533.5417187/1‑94 (MQ=60)

                                                                         GTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATC                      <  SRR4302533.837480/94‑1 (MQ=60)

                                                                          TCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCG                     <  SRR4302533.1188786/94‑1 (MQ=60)

                                                                          TCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCG                     <  SRR4302533.3936576/94‑1 (MQ=60)

                                                                          TCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCC                     >  SRR4302533.2429330/1‑94 (MQ=60)

                                                                           CTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGC                    >  SRR4302533.3957294/1‑94 (MQ=60)

                                                                           CTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGC                    >  SRR4302533.6079085/1‑94 (MQ=60)

                                                                            TGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCA                   <  SRR4302533.4502732/94‑1 (MQ=60)

                                                                              AAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAAT                 <  SRR4302533.5199555/94‑1 (MQ=60)

                                                                               AGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATC                <  SRR4302533.526765/94‑1 (MQ=60)

                                                                                GTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCA               >  SRR4302533.4930465/1‑94 (MQ=60)

                                                                                 TGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCAT              <  SRR4302533.4153911/94‑1 (MQ=60)

                                                                                 TGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCAT              <  SRR4302533.2643084/94‑1 (MQ=60)

                                                                                 TGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCAT              <  SRR4302533.2088184/94‑1 (MQ=60)

                                                                                 TGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCAT              <  SRR4302533.6029082/94‑1 (MQ=60)

                                                                                  GAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATG             >  SRR4302533.4612767/1‑94 (MQ=60)

                                                                                  GAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATG             <  SRR4302533.1372871/94‑1 (MQ=60)

                                                                                  GAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATG             <  SRR4302533.1102549/94‑1 (MQ=60)

                                                                                  GAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATG             <  SRR4302533.315526/94‑1 (MQ=60)

                                                                                   AAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGC            <  SRR4302533.2677002/94‑1 (MQ=60)

                                                                                    AATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCT           >  SRR4302533.5178456/1‑94 (MQ=60)

                                                                                     ATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCTG          <  SRR4302533.1618009/94‑1 (MQ=60)

                                                                                     ATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCTG          >  SRR4302533.5362886/1‑94 (MQ=60)

                                                                                      TTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCTGT         <  SRR4302533.5671698/94‑1 (MQ=60)

                                                                                      TTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCTGT         <  SRR4302533.4645110/94‑1 (MQ=60)

                                                                                        GGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCTGTTC       >  SRR4302533.1849758/1‑94 (MQ=60)

                                                                                         GCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCTGTTCT      <  SRR4302533.1556544/94‑1 (MQ=60)

                                                                                         GCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCTGTTCT      <  SRR4302533.2114752/94‑1 (MQ=60)

                                                                                         GCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCTGTTCT      <  SRR4302533.1051393/94‑1 (MQ=60)

                                                                                           CGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCTGTTCTTT    <  SRR4302533.1071241/94‑1 (MQ=60)

                                                                                            GCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCTGTTCTTTA   <  SRR4302533.500994/94‑1 (MQ=60)

                                                                                            GCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCTGTTCTTTA   <  SRR4302533.3750807/94‑1 (MQ=60)

                                                                                             CACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCTGTTCTTTAC  >  SRR4302533.5127170/1‑94 (MQ=60)

AAATCATAACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGTACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCTGTTCTTTAC  >  NC_000913/3731293‑3731479

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`