Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,737,956 2 bp→CG coding (623‑624/1254 nt) avtA → alanine‑alpha‑ketoisovalerate transaminase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,737,9560GC100.0% 732.4 / NA 227G208A (GGC→GCG) avtAalanine‑alpha‑ketoisovalerate transaminase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (118/109);  total (118/109)
*NC_0009133,737,9570CG100.0% 740.2 / NA 227G208A (GGC→GCG) avtAalanine‑alpha‑ketoisovalerate transaminase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (118/109);  total (118/109)

Only 100 of 236 total aligned reads displayed.

AACCGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGGCAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCTATG  >  NC_000913/3737870‑3738043
                                                                                      ||                                                                                      
 aCCGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCaa                                                                                  >  2:3160224/1‑93 (MQ=255)
   cGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAaca                                                                                >  2:1834277/1‑93 (MQ=255)
    gggATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTCCTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAacac                                                                               <  2:2802526/93‑1 (MQ=255)
    gggATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAacac                                                                               <  2:6112420/93‑1 (MQ=255)
     ggATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAAAACg                                                                              >  2:1242314/1‑93 (MQ=255)
     ggATGATTTGCGTCTACCGGCCGACGAATCCAACAGGCAATGGGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAAGACg                                                                              >  2:589474/1‑93 (MQ=255)
      gatgatTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACgg                                                                             >  1:3631004/1‑93 (MQ=255)
      gatgatTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACgg                                                                             <  1:1175589/93‑1 (MQ=255)
       atgatTTGCGTCTCCCGGCCGTCGAATCCAACAGGCAATGTGATTACTGACGAAGAGATGCTGACGCCTGACGCGCTGGCGAGTCAacagagc                                                                            >  2:4033352/1‑89 (MQ=255)
        tgatTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCa                                                                           >  2:5715576/1‑93 (MQ=255)
         gatTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCAt                                                                          <  2:5158388/93‑1 (MQ=255)
         gatTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCAt                                                                          <  2:1337906/93‑1 (MQ=255)
          atTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCAtt                                                                         >  1:799563/1‑93 (MQ=255)
            ttGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTcc                                                                       >  1:3807518/1‑93 (MQ=255)
                gTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTg                                                                   <  2:4387503/93‑1 (MQ=255)
                 tctcCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTgg                                                                  >  2:3372865/1‑93 (MQ=255)
                 tctcCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTgg                                                                  <  1:4714923/93‑1 (MQ=255)
                  ctcCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGt                                                                 <  1:3984934/93‑1 (MQ=255)
                     ccGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGtgat                                                              >  1:946982/1‑93 (MQ=255)
                        gCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGtgattga                                                           <  2:4502259/93‑1 (MQ=255)
                         ccGACGAATCCAACAGGCATTGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGtgattgat                                                          <  2:2534332/93‑1 (MQ=255)
                         ccGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGtgattgat                                                          <  2:530186/93‑1 (MQ=255)
                         ccGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGtgattgat                                                          >  2:4217686/1‑93 (MQ=255)
                         ccGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGtgattgat                                                          <  1:1573403/93‑1 (MQ=255)
                          cgacgaATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATa                                                         >  2:4301200/1‑93 (MQ=255)
                            acgaATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAAc                                                       >  1:1012871/1‑93 (MQ=255)
                             cgaATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACg                                                      <  1:1442890/93‑1 (MQ=255)
                             cgaATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACg                                                      >  1:5647928/1‑93 (MQ=255)
                              gaATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGc                                                     >  2:211126/1‑93 (MQ=255)
                              gaATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGc                                                     >  1:4173778/1‑93 (MQ=255)
                               aaTCCAACAGGCAATGTGATTACTGACTAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCt                                                    >  1:2382453/1‑93 (MQ=255)
                                aTCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCtt                                                   >  1:666812/1‑93 (MQ=255)
                                 tCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTa                                                  <  2:1536953/93‑1 (MQ=255)
                                  ccAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTAt                                                 <  2:1122195/93‑1 (MQ=255)
                                   cAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATg                                                <  2:2142581/93‑1 (MQ=255)
                                   cAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATg                                                >  2:1928153/1‑93 (MQ=255)
                                    aaCAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATgg                                               <  1:2205919/93‑1 (MQ=255)
                                    aaCAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATgg                                               >  1:5896018/1‑93 (MQ=255)
                                     aCAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATggg                                              >  1:5929859/1‑92 (MQ=255)
                                     aCAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGc                                              <  2:6179602/93‑1 (MQ=255)
                                      cAGGCAATGTGATTACTGACGAAGAGTTGCTGCAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCg                                             >  1:5343111/1‑93 (MQ=255)
                                       aGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGt                                            >  2:5068657/1‑93 (MQ=255)
                                       aGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGt                                            >  1:892150/1‑93 (MQ=255)
                                        ggCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTc                                           >  2:5231947/1‑93 (MQ=255)
                                        ggCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTc                                           <  2:2878580/93‑1 (MQ=255)
                                           actgtgATTACTGACGAAGAGTTGCTGAAGCTTGACGCGGTGGCGGATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTACCg                                        >  2:1402684/3‑93 (MQ=255)
                                           aaTGTGATTACTGACGAAGAGTTGCTGAAGCTTGTCGCGCTGGCGCATCACCCCGGCCTTCCGCTGGTGATTGCTAACGCTTATGGCGTCTCg                                        >  2:2397488/1‑93 (MQ=255)
                                           aaTGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCg                                        >  2:5546516/1‑93 (MQ=255)
                                           aaTGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAAAGCTTATGGCGTCCCg                                        >  1:3123112/1‑93 (MQ=255)
                                            aTGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGt                                       >  2:3451352/1‑93 (MQ=255)
                                            aTGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGt                                       >  1:28305/1‑93 (MQ=255)
                                             tgtgATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGtt                                      >  1:6169218/1‑93 (MQ=255)
                                             tgtgATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGtt                                      >  1:2753668/1‑93 (MQ=255)
                                                gATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTccc                                   <  2:4882490/93‑1 (MQ=255)
                                                  ttACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCgg                                 <  2:6248728/93‑1 (MQ=255)
                                                  ttACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTAGTGATTGATCACGCTTATGGCGTCCCGTTCCCgg                                 >  1:2565291/1‑93 (MQ=255)
                                                  atACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCACTGGTTATTGATAACGCTTATGGCGTCCCGTACCCgg                                 <  2:2029762/92‑1 (MQ=255)
                                                   tACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCggg                                <  1:4565592/93‑1 (MQ=255)
                                                    aCTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATg                                                >  2:169669/1‑76 (MQ=255)
                                                    aCTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATg                                                <  1:169669/76‑1 (MQ=255)
                                                    aCTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGt                               <  1:6034198/93‑1 (MQ=255)
                                                     cTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTa                              <  2:1795213/93‑1 (MQ=255)
                                                     cTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTa                              <  2:5380708/93‑1 (MQ=255)
                                                     cTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTACCGTTCCCGGGTa                              >  1:3438171/1‑93 (MQ=255)
                                                      tGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTat                             >  1:5520034/1‑93 (MQ=255)
                                                         ggaggagTTGCGGAAGCTTGACGCGCCGACGAATCAACGCGGCAGTCCGCTGGTGATTCATAACGCTTATCGCGTCCCGTTCCCGGGTatcat                          >  2:2728537/5‑93 (MQ=255)
                                                          gAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTatcatc                         >  1:3542015/1‑93 (MQ=255)
                                                          gAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTatcatc                         >  1:4435187/1‑93 (MQ=255)
                                                          gAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTatcatc                         <  1:1127048/93‑1 (MQ=255)
                                                           aaGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCt                        >  1:937694/1‑93 (MQ=255)
                                                              agTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCa                     <  1:1756156/93‑1 (MQ=255)
                                                              agTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCa                     >  1:2020596/1‑93 (MQ=255)
                                                              agTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCa                     >  1:4282139/1‑93 (MQ=255)
                                                              agTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCa                     <  1:4356420/93‑1 (MQ=255)
                                                                ttGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGt                   >  1:5228549/1‑93 (MQ=255)
                                                                ttGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGt                   >  1:3095583/1‑93 (MQ=255)
                                                                ttGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGt                   >  1:5900220/1‑93 (MQ=255)
                                                                 tGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTg                  >  1:733853/1‑93 (MQ=255)
                                                                 tGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGGGATTGATAACGCTTATGGCGTCCCGTTCCCGGGGATCATCTTCAGTg                  >  2:2735676/1‑93 (MQ=255)
                                                                  gCTGAAGCTTGACGCGCTGGCGAGTCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGa                 <  2:1170683/93‑1 (MQ=255)
                                                                   cTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGaa                <  1:1038575/93‑1 (MQ=255)
                                                                    tGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAg               <  2:4157648/93‑1 (MQ=255)
                                                                    tGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAg               <  2:3850434/93‑1 (MQ=255)
                                                                    tGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAg               <  2:3681801/93‑1 (MQ=255)
                                                                    tGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAg               >  2:2177972/1‑93 (MQ=255)
                                                                    tGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAg               >  2:5639995/1‑93 (MQ=255)
                                                                     gAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAgc              <  2:4974673/93‑1 (MQ=255)
                                                                     gAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAgc              >  1:4860610/1‑93 (MQ=255)
                                                                     gAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAgc              >  2:5852424/1‑93 (MQ=255)
                                                                     gAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAgc              <  1:5282896/93‑1 (MQ=255)
                                                                      acgCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAgcg             >  2:5008416/3‑93 (MQ=255)
                                                                       aGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAgcgc            <  2:635523/93‑1 (MQ=255)
                                                                        gCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAgcgcg           >  1:3279890/1‑93 (MQ=255)
                                                                        gCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAgcgcg           >  2:712156/1‑93 (MQ=255)
                                                                             aCGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGc      >  2:2691088/1‑93 (MQ=255)
                                                                              cgcgCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCt     <  2:6089982/93‑1 (MQ=255)
                                                                               gcgcTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCTa    >  1:5706913/1‑93 (MQ=255)
                                                                                cgcTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCTAt   <  2:3416895/93‑1 (MQ=255)
                                                                                 gcTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTCTGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCTATg  >  1:5042026/1‑93 (MQ=255)
                                                                                 gcTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCTATg  >  1:4640620/1‑93 (MQ=255)
                                                                                      ||                                                                                      
AACCGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGGCAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCTATG  >  NC_000913/3737870‑3738043

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AAACCGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGGCAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCTATGGA  >  NC_000913/3737869‑3738045
                                                                                       |                                                                                         
AAACCGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGCCGCGCTGGCGAATCA                                                                                     >  SRR4302533.3356293/1‑94 (MQ=60)
 AACCGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGCCGCGCTGGCGAATCAA                                                                                    >  SRR4302533.1526265/1‑94 (MQ=60)
 AACCGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAA                                                                                    <  SRR4302533.1782211/94‑1 (MQ=60)
  ACCGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAAC                                                                                   >  SRR4302533.5115352/1‑94 (MQ=60)
    CGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACAC                                                                                 <  SRR4302533.429037/94‑1 (MQ=60)
      GGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGG                                                                               >  SRR4302533.3653343/1‑94 (MQ=60)
       GATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGC                                                                              <  SRR4302533.1182643/94‑1 (MQ=60)
       GATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGC                                                                              <  SRR4302533.5523559/94‑1 (MQ=60)
       GATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGC                                                                              <  SRR4302533.714746/94‑1 (MQ=60)
        ATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGATGTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCA                                                                             >  SRR4302533.1598564/1‑94 (MQ=60)
         TGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCAT                                                                            >  SRR4302533.3987036/1‑94 (MQ=60)
          GATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATT                                                                           >  SRR4302533.804267/1‑94 (MQ=60)
            TTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCC                                                                         >  SRR4302533.3830811/1‑94 (MQ=60)
                  TCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGT                                                                   <  SRR4302533.4743498/94‑1 (MQ=60)
                  TCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGT                                                                   >  SRR4302533.4407221/1‑94 (MQ=60)
                   CTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTG                                                                  <  SRR4302533.4009196/94‑1 (MQ=60)
                     CCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGAT                                                                <  SRR4302533.5267273/94‑1 (MQ=60)
                     CCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGAT                                                                >  SRR4302533.952550/1‑94 (MQ=60)
                       CGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTG                                                              <  SRR4302533.876915/94‑1 (MQ=60)
                          CCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATA                                                           <  SRR4302533.1582877/94‑1 (MQ=60)
                          CCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATA                                                           <  SRR4302533.2242659/94‑1 (MQ=60)
                          CCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATA                                                           <  SRR4302533.5935602/94‑1 (MQ=60)
                            GACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAAC                                                         >  SRR4302533.1018801/1‑94 (MQ=60)
                             ACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACG                                                        <  SRR4302533.6186106/94‑1 (MQ=60)
                             ACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACG                                                        >  SRR4302533.5682070/1‑94 (MQ=60)
                              CGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGC                                                       >  SRR4302533.4199071/1‑94 (MQ=60)
                              CGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGC                                                       <  SRR4302533.1451594/94‑1 (MQ=60)
                               GAATCCAACAGGCAATGTGATTACTGACTAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCT                                                      >  SRR4302533.2397185/1‑94 (MQ=60)
                                AATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTT                                                     >  SRR4302533.670751/1‑94 (MQ=60)
                                AATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTT                                                     <  SRR4302533.4780288/94‑1 (MQ=60)
                                  TCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTAT                                                   <  SRR4302533.877534/94‑1 (MQ=60)
                                  TCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTAT                                                   <  SRR4302533.5163228/94‑1 (MQ=60)
                                   CCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATG                                                  >  SRR4302533.1161752/1‑94 (MQ=60)
                                   CCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATG                                                  <  SRR4302533.4128445/94‑1 (MQ=60)
                                   CCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATG                                                  >  SRR4302533.5462139/1‑94 (MQ=60)
                                    ttccgatctgctgtATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGG                                                 <  SRR4302533.170740/80‑1 (MQ=60)
                                    CAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGG                                                 >  SRR4302533.6069462/1‑94 (MQ=60)
                                    CAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGG                                                 >  SRR4302533.949821/1‑94 (MQ=60)
                                    CAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGG                                                 >  SRR4302533.5931622/1‑94 (MQ=60)
                                     AACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGG                                                >  SRR4302533.5965708/1‑94 (MQ=60)
                                     AACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGC                                                >  SRR4302533.5965707/1‑94 (MQ=60)
                                     AACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGC                                                <  SRR4302533.2219523/94‑1 (MQ=60)
                                     AACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCCTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGC                                                <  SRR4302533.2053284/94‑1 (MQ=60)
                                      ACAGGCAATGTGATTACTGACGAAGAGTTGCTGCAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCG                                               >  SRR4302533.5375152/1‑94 (MQ=60)
                                      ACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCG                                               <  SRR4302533.2800281/94‑1 (MQ=60)
                                       CAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGT                                              >  SRR4302533.897392/1‑94 (MQ=60)
                                       CAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGT                                              >  SRR4302533.2632532/1‑94 (MQ=60)
                                       CAGGCAATGGGATTACTGACGAAGAGCTGCTGCAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGT                                              >  SRR4302533.587799/1‑94 (MQ=60)
                                          GCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCC                                           >  SRR4302533.5357669/1‑94 (MQ=60)
                                           CAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCG                                          <  SRR4302533.5691092/94‑1 (MQ=60)
                                           CAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAAAGCTTATGGCGTCCCG                                          >  SRR4302533.3142313/1‑94 (MQ=60)
                                            AATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGT                                         >  SRR4302533.28497/1‑94 (MQ=60)
                                             ATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTT                                        >  SRR4302533.6206301/1‑94 (MQ=60)
                                             ATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTT                                        >  SRR4302533.2770555/1‑94 (MQ=60)
                                             ATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGGGATTGATAACGCTTATGGCGTCCCGTT                                        >  SRR4302533.2983757/1‑94 (MQ=60)
                                               GTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCC                                      <  SRR4302533.2944900/94‑1 (MQ=60)
                                               GTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCC                                      >  SRR4302533.1262424/1‑94 (MQ=60)
                                                TGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCC                                     <  SRR4302533.748636/94‑1 (MQ=60)
                                                  ATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGG                                   <  SRR4302533.2148521/94‑1 (MQ=60)
                                                  ATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTAGTGATTGATCACGCTTATGGCGTCCCGTTCCCGG                                   >  SRR4302533.2581078/1‑94 (MQ=60)
                                                   TTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGG                                  >  SRR4302533.2422874/1‑94 (MQ=60)
                                                    TACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGT                                 <  SRR4302533.4593272/94‑1 (MQ=60)
                                                    TACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGT                                 <  SRR4302533.1036681/94‑1 (MQ=60)
                                                     ACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTA                                <  SRR4302533.6070764/94‑1 (MQ=60)
                                                     ACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTACCGTTCCCGGGTA                                >  SRR4302533.3459404/1‑94 (MQ=60)
                                                      CTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTAT                               >  SRR4302533.5565806/1‑94 (MQ=60)
                                                      CTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTAT                               >  SRR4302533.5553365/1‑94 (MQ=60)
                                                      CTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTAT                               <  SRR4302533.967952/94‑1 (MQ=60)
                                                         ACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCAT                            <  SRR4302533.195196/94‑1 (MQ=60)
                                                         ACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCAT                            >  SRR4302533.4506721/1‑94 (MQ=60)
                                                          CGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTTCGCTGGTGATTGATAACGCTCATGGCGTCCCGTTCCCGGGTATCATC                           >  SRR4302533.2606656/1‑94 (MQ=60)
                                                          CGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATC                           >  SRR4302533.4461997/1‑94 (MQ=60)
                                                          CGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATC                           >  SRR4302533.6245930/1‑94 (MQ=60)
                                                          CGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATC                           >  SRR4302533.3563841/1‑94 (MQ=60)
                                                           GAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCT                          <  SRR4302533.1133742/94‑1 (MQ=60)
                                                           GAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCT                          >  SRR4302533.5754505/1‑94 (MQ=60)
                                                           GAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCT                          >  SRR4302533.943202/1‑94 (MQ=60)
                                                            AAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTT                         >  SRR4302533.905372/1‑94 (MQ=60)
                                                             AGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTC                        >  SRR4302533.4623244/1‑94 (MQ=60)
                                                              GAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCA                       <  SRR4302533.1734392/94‑1 (MQ=60)
                                                              GAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCA                       <  SRR4302533.4711988/94‑1 (MQ=60)
                                                              GAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCA                       <  SRR4302533.432526/94‑1 (MQ=60)
                                                              GAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCA                       >  SRR4302533.4307876/1‑94 (MQ=60)
                                                              GAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCA                       >  SRR4302533.2033039/1‑94 (MQ=60)
                                                               AGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAG                      <  SRR4302533.4382715/94‑1 (MQ=60)
                                                               AGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAG                      <  SRR4302533.1766854/94‑1 (MQ=60)
                                                                GTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGT                     >  SRR4302533.5260003/1‑94 (MQ=60)
                                                                GTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGT                     >  SRR4302533.5935852/1‑94 (MQ=60)
                                                                GTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGT                     >  SRR4302533.3114613/1‑94 (MQ=60)
                                                                 TTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTG                    >  SRR4302533.738171/1‑94 (MQ=60)
                                                                 TTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTG                    >  SRR4302533.3129093/1‑94 (MQ=60)
                                                                   GCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAA                  <  SRR4302533.3689785/94‑1 (MQ=60)
                                                                   GCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAA                  <  SRR4302533.1656945/94‑1 (MQ=60)
                                                                    CTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAG                 <  SRR4302533.1044678/94‑1 (MQ=60)
                                                                     TGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGC                >  SRR4302533.4890070/1‑94 (MQ=60)
                                                                     TGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGC                >  SRR4302533.3543382/1‑94 (MQ=60)
                                                                     TGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGC                <  SRR4302533.4439556/94‑1 (MQ=60)
                                                                      GAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCG               <  SRR4302533.1045094/94‑1 (MQ=60)
                                                                      GAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCG               <  SRR4302533.5314543/94‑1 (MQ=60)
                                                                      GAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCG               <  SRR4302533.5940829/94‑1 (MQ=60)
                                                                       AAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGC              <  SRR4302533.3198199/94‑1 (MQ=60)
                                                                       AAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGC              <  SRR4302533.4202207/94‑1 (MQ=60)
                                                                        AGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCG             >  SRR4302533.3429948/1‑94 (MQ=60)
                                                                        AGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCG             >  SRR4302533.3300139/1‑94 (MQ=60)
                                                                         GCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGC            >  SRR4302533.3656652/1‑94 (MQ=60)
                                                                            TGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCG         >  SRR4302533.6211578/1‑94 (MQ=60)
                                                                            TGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCG         >  SRR4302533.4703813/1‑94 (MQ=60)
                                                                             GACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGC        <  SRR4302533.1181392/94‑1 (MQ=60)
                                                                               CGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCTA      >  SRR4302533.5741384/1‑94 (MQ=60)
                                                                                 CGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTCTGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCTATG    >  SRR4302533.5072541/1‑94 (MQ=60)
                                                                                 CGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCTATG    >  SRR4302533.4668736/1‑94 (MQ=60)
                                                                                  GCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCTATGG   <  SRR4302533.4895769/94‑1 (MQ=60)
                                                                                   CTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCTATGGA  <  SRR4302533.471474/94‑1 (MQ=60)
                                                                                       |                                                                                         
AAACCGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGGCAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCTATGGA  >  NC_000913/3737869‑3738045

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑