Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,779,837	T→C	H118R (CAC→CGC)	cysE ←	serine acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,779,837	0	T	C	100.0%	853.7 / NA	266	H118R (CAC→CGC)	cysE	serine acetyltransferase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (131/135); total (131/135)

Only 100 of 271 total aligned reads displayed.

GCTGCCGGGTGAATATCGACCTGGAACGTCACAGAAACCTGGTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGTGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGGTATAACAACGGGGTTGAGTATTTATCGACTGCCGGGTCGCGGGTACGC  >  NC_000913/3779747‑3779926

 ctgcCGGGTGAATATCGACCTGGAACGTCACAGAAACCTGGTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGAc                                                                                        <  2:1156111/93‑1 (MQ=255)

   gcCGGGTGAATATCGACCTGGAACGTCACAGAAACCTGGTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCg                                                                                      >  2:2805620/1‑93 (MQ=255)

   gcCGGGTGAATATCGACCTGGAACGTCACAGAAACCTGGTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCg                                                                                      >  2:2330807/1‑93 (MQ=255)

   gcCGGGTGAATATCGACCTGGAACGTCACAGAAACCTGGTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCg                                                                                      >  2:5455875/1‑93 (MQ=255)

     cGGGTGAATATCGACCTGGAACGTCACAGAAACCTGGTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGAt                                                                                    <  1:1682522/93‑1 (MQ=255)

       ggTGAATATCGACCTGGAACGTCACAGAAACCTGGTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGc                                                                                  >  1:4604262/1‑93 (MQ=255)

       ggTGAATATCGACCTGGAACGTCACAGAAACCTGGTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGc                                                                                  <  2:4676086/93‑1 (MQ=255)

        gTGAATATCGACCTGGAACGTCACAGAAACCTGGTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCg                                                                                 >  1:5105378/1‑93 (MQ=255)

         tGAATATCGACCTGGAACGTCACAGAAACCTGGTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGa                                                                                <  1:3781660/93‑1 (MQ=255)

           aaTATCGACCTGGAACGTCACAGAAACCTGGTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATa                                                                              <  2:5374406/93‑1 (MQ=255)

              atCGACCTGGAACGTCACAGAAACCTGGTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGc                                                                           <  2:2653077/93‑1 (MQ=255)

               tCGACCTGGAACGTCACAGAAACCTGGTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGcc                                                                          >  1:5760135/1‑93 (MQ=255)

                cGACCTGGAACGTCACAGAAACCTGGTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCt                                                                         >  1:677766/1‑93 (MQ=255)

                cGACCTGGAACGTCACAGAAACCTGGTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCt                                                                         <  2:3508875/93‑1 (MQ=255)

                  aCCTGGAACGTCACAGAAACCTGGTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGc                                                                       >  1:5443182/1‑93 (MQ=255)

                  aCCTGGAACGTCACAGAAACCTGGTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGc                                                                       <  2:4422111/93‑1 (MQ=255)

                  aCCTGGAACGTCACAGAAACCTGGTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGc                                                                       >  1:1378392/1‑93 (MQ=255)

                  aCCTGGAACGTCACAGAAACCTGGTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGc                                                                       <  2:1296285/93‑1 (MQ=255)

                    cTGGAACGTCACAGAAACCTGGTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCaa                                                                     >  2:5971445/1‑93 (MQ=255)

                    cTGGAACGTCACAGAAACCTGGTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCaa                                                                     >  2:4751104/1‑93 (MQ=255)

                    cTGGAACGTCACAGAAACCTGGTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCaa                                                                     <  1:4161757/93‑1 (MQ=255)

                    cTGGAACGTCACAGAAACCTGGTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCaa                                                                     >  2:2948538/1‑93 (MQ=255)

                     tGGAACGTCACAGAAACCTGGTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGTCTGCAAg                                                                    >  2:2398534/1‑93 (MQ=255)

                     tGGAACGTCACAGAAACCTGGTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAg                                                                    >  1:2215305/1‑93 (MQ=255)

                      ggAACGTCACAGAAACCTGGTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAgg                                                                   >  2:861393/1‑93 (MQ=255)

                       gAACGTCACAGAAACCTGGTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGc                                                                  <  2:3591560/93‑1 (MQ=255)

                       gAACGTCACAGAAACCTGGTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGc                                                                  >  1:793222/1‑93 (MQ=255)

                       gAACGTCACAGAAACCTGGTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGc                                                                  >  2:1530589/1‑93 (MQ=255)

                        aaCGTCACAGAAACCTGGTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCa                                                                 <  2:3438349/93‑1 (MQ=255)

                        aaCGTCACAGAAACCTGGTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCa                                                                 >  2:6193661/1‑93 (MQ=255)

                         aCGTCACAGAAACCTGGTTTTGCAGAAAGATTGCCAGTGCGCGACGCCGCTGATTCCACAACCACCGACCGATGCGATAGGCCTGCAAGGCAt                                                                >  1:538000/1‑93 (MQ=255)

                         aCGTCACAGAAACCTGGTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCAt                                                                <  2:1351667/93‑1 (MQ=255)

                         aCGTCACAGAAACCTGGTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCAt                                                                <  2:4048096/93‑1 (MQ=255)

                           gTCACAGAAACCTGGTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGa                                                              >  2:2629374/1‑93 (MQ=255)

                           gTCACAGAAACCTGGTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGa                                                              >  2:4285407/1‑93 (MQ=255)

                              acaGAAACCTGGTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGaaaa                                                           >  2:1801078/1‑93 (MQ=255)

                               caGAAACCTGGTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAAc                                                          >  2:702071/1‑93 (MQ=255)

                                 gAAACCTGGTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAAccc                                                        >  2:1129655/1‑93 (MQ=255)

                                 gAAACCTGGTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAAccc                                                        <  1:4789552/93‑1 (MQ=255)

                                  aaaCCTGGTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCt                                                       >  1:4221946/1‑93 (MQ=255)

                                  aaaCCTGGTTTTGCAGAAAGATTGCCAGGGCCCGACCCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCt                                                       <  1:1611239/93‑1 (MQ=255)

                                    aCCTGGTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTc                                                     <  2:1980777/93‑1 (MQ=255)

                                    aCCTGGTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTc                                                     <  2:4902260/93‑1 (MQ=255)

                                     ccTGGTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCa                                                    <  1:3713902/93‑1 (MQ=255)

                                      cTGGTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAg                                                   <  1:6151586/93‑1 (MQ=255)

                                      cTGGTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAg                                                   >  1:3358787/1‑93 (MQ=255)

                                       tGGTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAgg                                                  <  1:1518714/93‑1 (MQ=255)

                                       tGGTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAgg                                                  >  1:5871897/1‑93 (MQ=255)

                                       tGGTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAgg                                                  >  1:6213335/1‑93 (MQ=255)

                                       tGGTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAgg                                                  >  1:1288183/1‑93 (MQ=255)

                                        ggTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGGt                                                 <  2:4313715/93‑1 (MQ=255)

                                        ggTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGGt                                                 >  2:1095393/1‑93 (MQ=255)

                                         gTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGGta                                                >  1:2040329/1‑93 (MQ=255)

                                           tttGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGGtata                                              >  2:6282874/1‑93 (MQ=255)

                                            ttGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGGTATaa                                             <  2:2909897/93‑1 (MQ=255)

                                             tGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGGTATaac                                            >  1:4723811/1‑93 (MQ=255)

                                               cAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAGCCCTTCAGGTATaacaa                                          <  2:5068299/93‑1 (MQ=255)

                                               cAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGGTATaacaa                                          <  2:3822405/93‑1 (MQ=255)

                                                aGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGACACCCCTTCAGGTATaacaac                                         >  2:4521585/1‑93 (MQ=255)

                                                 gAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGGTATAACAACg                                        >  2:3068729/1‑93 (MQ=255)

                                                 gAAAGATTGCCAGTCCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGGTATAACAACg                                        <  1:2358960/93‑1 (MQ=255)

                                                   aaGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGGTATAACAACggg                                      >  2:5284544/1‑93 (MQ=255)

                                                   aaGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGGTATAACAACggg                                      <  1:1033657/93‑1 (MQ=255)

                                                    aGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGGTATAACAACgggg                                     >  1:6170241/1‑93 (MQ=255)

                                                     gATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGGTATAACAACGGGGt                                    <  2:5783550/93‑1 (MQ=255)

                                                     gATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGGTATAACAACGGGGt                                    <  2:4833766/93‑1 (MQ=255)

                                                     gATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGGTATAACAACGGGGt                                    >  2:1629858/1‑93 (MQ=255)

                                                       ttGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGGTATAACAACGGGGTTg                                  >  1:71030/1‑93 (MQ=255)

                                                       ttGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGGTATAACAACGGGGTTg                                  >  1:4079093/1‑93 (MQ=255)

                                                       ttGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGGTATAACAACGGGGTTg                                  >  1:808380/1‑93 (MQ=255)

                                                        tGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGGTATAACAACGGGGTTGa                                 >  2:1500043/1‑93 (MQ=255)

                                                        tGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGGTATAACAACGGGGTTGa                                 <  2:5906386/93‑1 (MQ=255)

                                                         gCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGGTATAACAACGGGGTTGAg                                >  1:6277368/1‑93 (MQ=255)

                                                         gCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGGTATAACAACGGGGTTGAg                                <  1:2545956/93‑1 (MQ=255)

                                                           cAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGGTATAACAACGGGGTTGAGTa                              >  1:2941043/1‑93 (MQ=255)

                                                            aGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGGTATAACAACGGGGTTGAGTAt                             <  1:327223/93‑1 (MQ=255)

                                                             atGCGCGACGCCCCTAATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACACTTCAGGTATAACAACGGGGTTGAGTAtt                            <  2:2070017/92‑1 (MQ=255)

                                                              tGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGGTATAACAACGGGGTTGAGTAttt                           >  2:78567/1‑93 (MQ=255)

                                                               gcggGACGCCCCCGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAACCCCTTCAGGTATAACAACGGGGTTGAGTATTTa                          <  2:4366864/93‑1 (MQ=255)

                                                               gcgcGACTCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGGTATAACAACGGGGTTGAGTATTTa                          <  1:3682863/93‑1 (MQ=255)

                                                                cgcgACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGGTATAACAACGGGGTTGAGTATTTAt                         <  1:2846464/93‑1 (MQ=255)

                                                                cgcgACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGGTATAACAACGGGGTTGAGTATTTAt                         >  1:2932970/1‑93 (MQ=255)

                                                                   gACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGGTATAACAACGGGGTTGAGTATTTATCGa                      <  1:3528389/93‑1 (MQ=255)

                                                                    aCGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGGTATAACAACGGGGTTGAGTATTTATCGAc                     <  2:2208997/93‑1 (MQ=255)

                                                                      gCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGGTATAACAACGGGGTTGAGTATGTATCGACTg                   >  1:384550/1‑93 (MQ=255)

                                                                        cccTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGGTATAACAACGGGGTTGAGTATTTATCGACTGcc                 <  2:5224758/93‑1 (MQ=255)

                                                                          cTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGGTATAACAACGGGGTTGAGTATTTATCGACTGCCgg               >  2:3261676/1‑93 (MQ=255)

                                                                          cTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGGTATAACAACGGGGTTGAGTATTTATCGACTGCCgg               <  2:1825946/93‑1 (MQ=255)

                                                                           tGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGGTATAACAACGGGGTTGAGTATTTATCGACTGCCggg              <  1:2467120/93‑1 (MQ=255)

                                                                              ttCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGGTATAACAACGGGGATGAGTATTTATCGACTGCCGGGTcg           >  1:1486491/1‑93 (MQ=255)

                                                                               tCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGGTATAACAACGGGGTTGAGTATTTATCGACTGCCGGGTcgc          <  2:581797/93‑1 (MQ=255)

                                                                                 cacaACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGGTATAACAACGGGGTTGAGTATTTATCGACTGCCGGGTCGCgg        <  1:2586980/93‑1 (MQ=255)

                                                                                 cacaACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGGTATAACAACGGGGTTGAGTATTTATCGACTGCCGGGTCGCgg        >  1:4390122/1‑93 (MQ=255)

                                                                                 cacaACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGGTATAACAACGGGGTTGAGTATTTATCGACTGCCGGGTCGCgg        <  1:889974/93‑1 (MQ=255)

                                                                                    aaCCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGGTATAACAACGGGGTTGAGTATTTATCGACTGCCGGGTCGCGGGTa     <  1:3973292/93‑1 (MQ=255)

                                                                                    aaCCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGGTATAACAACGGGGTTGAGTATTTATCGACTGCCGGGTCGCGGGTa     <  1:4341830/93‑1 (MQ=255)

                                                                                     aCCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGGTATAACAACGGGGTTGAGTATTTATCGACTGCCGGGTCGCGGGTAc    <  1:2752601/93‑1 (MQ=255)

                                                                                      ccAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGGTATAACAACGGGGTTGAGTATTTATCGACTGCCGGGTCGCGGGTACg   <  1:3072002/93‑1 (MQ=255)

                                                                                      ccAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGGTATAACAACGGGGTTGAGTATTTATCGACTGCCGGGTCGCGGGTACg   >  1:1864412/1‑93 (MQ=255)

                                                                                       cAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGGTATAACAACGGGGTTGAGTATTTATCGACTGCCGGGTCGCGGGTACGc  >  1:1133331/1‑93 (MQ=255)

GCTGCCGGGTGAATATCGACCTGGAACGTCACAGAAACCTGGTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGTGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGGTATAACAACGGGGTTGAGTATTTATCGACTGCCGGGTCGCGGGTACGC  >  NC_000913/3779747‑3779926

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TTTGCTGCCGGGTGAATATCGACCTGGAACGTCACAGAAACCTGGTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGTGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGGTATAACAACGGGGTTGAGTATTTATCGACTGCCGGGTCGCGGGTACGCACCG  >  NC_000913/3779744‑3779930

TTTGCTGCCGGGTGAATATCGAGCTGGAACGTCACAGAAACCTGGTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGC                                                                                               >  SRR4302533.31772/1‑94 (MQ=60)

 TTGCTGCCGGGTGAATATCGACCTGGAACGTCACAGAAACCTGGTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCG                                                                                              <  SRR4302533.3251393/94‑1 (MQ=60)

 TTGCTGCCGGGTGAATATCGACCTGGAACGTCACAGAAACCTGGTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCG                                                                                              <  SRR4302533.2633615/94‑1 (MQ=60)

  TGCTGCCGGGTGAATATCGACCTGGAACGTCACAGAAACCTGGTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGA                                                                                             <  SRR4302533.1033638/94‑1 (MQ=60)

  TGCTGCCGGGTGAATATCGACCTGGAACGTCACAGAAACCTGGTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGA                                                                                             <  SRR4302533.4310245/94‑1 (MQ=60)

   GCTGCCGGGTGAATATCGACCTGGAACGTCACAGAAAACTGGTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGAC                                                                                            <  SRR4302533.4641836/94‑1 (MQ=60)

    CTGCCGGGTGAATATCGACCTGGAACGTCACAGAAACCTGGTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACC                                                                                           <  SRR4302533.5437396/94‑1 (MQ=60)

     TGCCGGGTGAATATCGACCTGGAACGTCACAGAAACCTGGTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCG                                                                                          >  SRR4302533.3587702/1‑94 (MQ=60)

        CGGGTGAATATCGACCTGGAACGTCACAGAAACCTGGTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATG                                                                                       <  SRR4302533.1692712/94‑1 (MQ=60)

         GGGTGAATATCGACCTGGAACGTCACAGAAACCTGGTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGC                                                                                      >  SRR4302533.2443811/1‑94 (MQ=60)

         GGGTGAATATCGACCTGGAACGTCACAGAAACCTGGTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGC                                                                                      >  SRR4302533.4632155/1‑94 (MQ=60)

         GGGTGAATATCGACCTGGAACGTCACAGAAACCTGGTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGC                                                                                      <  SRR4302533.3306973/94‑1 (MQ=60)

          GGTGAATATCGACCTGGAACGTCACAGAAACCTGGTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCG                                                                                     >  SRR4302533.5136217/1‑94 (MQ=60)

          GGTGAATATCGACCTGGAACGTCACAGAAACCTGGTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCG                                                                                     >  SRR4302533.3947949/1‑94 (MQ=60)

           GTGAATATCGACCTGGAACGTCACAGAAACCTGGTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGA                                                                                    >  SRR4302533.3017325/1‑94 (MQ=60)

            TGAATATCGACCTGGAACGTCACAGAAACCTGGTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGAT                                                                                   <  SRR4302533.3804822/94‑1 (MQ=60)

            TGAATATCGACCTGGAACGTCACAGAAACCTGGTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGAT                                                                                   <  SRR4302533.782022/94‑1 (MQ=60)

             GAATATCGACCTGGAACGTCACAGAAACCTGGTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATA                                                                                  <  SRR4302533.407387/94‑1 (MQ=60)

              AATATCGACCTGGAACGTCACAGAAACCTGGTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAG                                                                                 <  SRR4302533.4194610/94‑1 (MQ=60)

              AATATCGACCTGGAACGTCACAGAAACCTGGTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAG                                                                                 <  SRR4302533.704945/94‑1 (MQ=60)

               ATATCGACCTGGAACGTCACAGAAACCTGGTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGG                                                                                >  SRR4302533.328796/1‑94 (MQ=60)

               ATATCGACCTGGAACGTCACAGAAACCTGGTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGG                                                                                >  SRR4302533.620141/1‑94 (MQ=60)

                TATCGACCTGGAACGTCACAGAAACCTGGTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGC                                                                               >  SRR4302533.5311058/1‑94 (MQ=60)

                 ATCGACCTGGAACGTCACAGAAACCTGGTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCC                                                                              <  SRR4302533.3825405/94‑1 (MQ=60)

                 ATCGACCTGGAACGTCACAGAAACCTGGTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCC                                                                              >  SRR4302533.5794893/1‑94 (MQ=60)

                 ATCGACCTGGAACGTCACAGAAACCTGGTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCC                                                                              <  SRR4302533.4541135/94‑1 (MQ=60)

                 ATCGACCTGGAACGTCACAGAAACCTGGTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCC                                                                              >  SRR4302533.5935873/1‑94 (MQ=60)

                 ATCGACCTGGAACGTCACAGAAACCTGGTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCC                                                                              <  SRR4302533.5752794/94‑1 (MQ=60)

                  TCGACCTGGAACGTCACAGAAACCTGGTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCT                                                                             >  SRR4302533.681773/1‑94 (MQ=60)

                    GACCTGGAACGTCACAGAAACCTGGTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGC                                                                           >  SRR4302533.1386685/1‑94 (MQ=60)

                    GACCTGGAACGTCACAGAAACCTGGTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGC                                                                           >  SRR4302533.1963716/1‑94 (MQ=60)

                    GACCTGGAACGTCACAGAAACCTGGTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGC                                                                           >  SRR4302533.5475973/1‑94 (MQ=60)

                      CCTGGAACGTCACAGAAACCTGGTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAA                                                                         >  SRR4302533.4767355/1‑94 (MQ=60)

                       CTGGAACGTCACAGAAACCTGGTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAG                                                                        <  SRR4302533.37787/94‑1 (MQ=60)

                       CTGGAACGTCACAGAAACCTGGTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAG                                                                        <  SRR4302533.4186975/94‑1 (MQ=60)

                       CTGGAACGTCACAGAAACCTGGTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAG                                                                        >  SRR4302533.2228979/1‑94 (MQ=60)

                         GGAACGTCACAGAAACCTGGTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGC                                                                      <  SRR4302533.2957623/94‑1 (MQ=60)

                         GGAACGTCACAGAAACCTGGTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGC                                                                      >  SRR4302533.797890/1‑94 (MQ=60)

                         GGAACGTCACAGAAACCTGGTATTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGC                                                                      <  SRR4302533.1837534/94‑1 (MQ=60)

                          GAACTTCACAGAATCCTGGTTTTGCAGAAAGATTGGCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCA                                                                     <  SRR4302533.1202802/94‑1 (MQ=60)

                           AACGTCACAGAAACCTGGTTTTGCAGAAAGATTGCCAGTGCGCGACGCCGCTGATTCCACAACCACCGACCGATGCGATAGGCCTGCAAGGCAT                                                                    >  SRR4302533.541200/1‑94 (MQ=60)

                           AACGTCACAGAAACCTGGTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCAT                                                                    <  SRR4302533.2533685/94‑1 (MQ=60)

                           AACGTCACAGAAACCTGGTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCAT                                                                    <  SRR4302533.3892735/94‑1 (MQ=60)

                            ACGTCACAGAAACCTGGTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATG                                                                   >  SRR4302533.1180807/1‑94 (MQ=60)

                            ACGTCACAGAAACCTGGTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATG                                                                   <  SRR4302533.2796664/94‑1 (MQ=60)

                             CGTCACAGAAACCTGGTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGA                                                                  >  SRR4302533.25349/1‑94 (MQ=60)

                              GTCACAGAAACCTGGTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAA                                                                 >  SRR4302533.3413039/1‑94 (MQ=60)

                                CACAGAAACCTGGTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAA                                                               <  SRR4302533.6264404/94‑1 (MQ=60)

                                  CAGAAACCTGGTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACC                                                             >  SRR4302533.3029299/1‑94 (MQ=60)

                                   AGAAACCTGGTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCC                                                            <  SRR4302533.5030241/94‑1 (MQ=60)

                                    GAAACCTGGTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCT                                                           <  SRR4302533.4818565/94‑1 (MQ=60)

                                    GAAACCTGGTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCT                                                           >  SRR4302533.4247475/1‑94 (MQ=60)

                                     AAACCTGGTTTTGCAGAAAGATTGCCAGGGCCCGACCCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTT                                                          <  SRR4302533.1621009/94‑1 (MQ=60)

                                       ACCTGGTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCA                                                        <  SRR4302533.4215628/94‑1 (MQ=60)

                                       ACCTGGTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCA                                                        >  SRR4302533.3817799/1‑94 (MQ=60)

                                       ACCTGGTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCA                                                        >  SRR4302533.2080899/1‑94 (MQ=60)

                                        CCTGGTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAG                                                       <  SRR4302533.3736704/94‑1 (MQ=60)

                                        CCTGGTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAG                                                       >  SRR4302533.3379541/1‑94 (MQ=60)

                                         CTGGTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGG                                                      >  SRR4302533.6250592/1‑94 (MQ=60)

                                         CTGGTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGG                                                      >  SRR4302533.5907391/1‑94 (MQ=60)

                                         CTGGTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGG                                                      >  SRR4302533.1295934/1‑94 (MQ=60)

                                         CTGGTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGG                                                      <  SRR4302533.6229546/94‑1 (MQ=60)

                                         CTGGTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGG                                                      >  SRR4302533.2874371/1‑94 (MQ=60)

                                         CTGGTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGG                                                      <  SRR4302533.6188608/94‑1 (MQ=60)

                                          TGGTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGGT                                                     <  SRR4302533.150234/94‑1 (MQ=60)

                                          TGGTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGGT                                                     <  SRR4302533.1527856/94‑1 (MQ=60)

                                           GGTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGGTA                                                    >  SRR4302533.2052907/1‑94 (MQ=60)

                                           GGTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGGTA                                                    >  SRR4302533.820375/1‑94 (MQ=60)

                                           GGTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGGTA                                                    <  SRR4302533.1386177/94‑1 (MQ=60)

                                            GTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGGTAT                                                   >  SRR4302533.491251/1‑94 (MQ=60)

                                            GTTTTGCAGAAAGATTGCCAGTACGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGGTAT                                                   >  SRR4302533.2353510/1‑94 (MQ=60)

                                               TTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGGTATAAC                                                >  SRR4302533.4752454/1‑94 (MQ=60)

                                                TGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGGTATAACA                                               <  SRR4302533.801466/94‑1 (MQ=60)

                                                  CAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGGTATAACAAC                                             >  SRR4302533.1766601/1‑94 (MQ=60)

                                                   AGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAACCCCTTCAGGTATAACAACG                                            <  SRR4302533.3509267/94‑1 (MQ=60)

                                                   AGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGGTATAACAACG                                            <  SRR4302533.373575/94‑1 (MQ=60)

                                                   AGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGGTATAACAACG                                            <  SRR4302533.2421894/94‑1 (MQ=60)

                                                   AGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGGTATAACAACG                                            >  SRR4302533.451687/1‑94 (MQ=60)

                                                    GAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGGTATAACAACGG                                           >  SRR4302533.1446296/1‑94 (MQ=60)

                                                    GAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGGTATAACAACGG                                           >  SRR4302533.4261637/1‑94 (MQ=60)

                                                    GAAAGATTGCCAGTCCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGGTATAACAACGG                                           <  SRR4302533.2373539/94‑1 (MQ=60)

                                                      AAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGGTATAACAACGGGG                                         >  SRR4302533.6207326/1‑94 (MQ=60)

                                                      AAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGGTATAACAACGGGG                                         <  SRR4302533.1039738/94‑1 (MQ=60)

                                                       AGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGGTATAACAACGGGGT                                        >  SRR4302533.4676226/1‑94 (MQ=60)

                                                        GATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGGTATAACAACGGGGTT                                       >  SRR4302533.5641816/1‑94 (MQ=60)

                                                         ATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGGTATAACAACGGGGTTG                                      >  SRR4302533.4103925/1‑94 (MQ=60)

                                                         ATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGGTATAACAACGGGGTTG                                      >  SRR4302533.813130/1‑94 (MQ=60)

                                                         ATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGGTATAACAACGGGGTTG                                      >  SRR4302533.71488/1‑94 (MQ=60)

                                                         ATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGGTATAACAACGGGGTTG                                      >  SRR4302533.428671/1‑94 (MQ=60)

                                                         ATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGGTATAACAACGGGGTTG                                      >  SRR4302533.5342939/1‑94 (MQ=60)

                                                           TGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGGTATAACAACGGGGTTGAG                                    <  SRR4302533.4172397/94‑1 (MQ=60)

                                                           TGCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGGTATAACAACGGGGTTGAG                                    >  SRR4302533.6314848/1‑94 (MQ=60)

                                                            GCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGGTATAACAACGGGGTTGAGT                                   <  SRR4302533.2561620/94‑1 (MQ=60)

                                                            GCCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGGTATAACAACGGGGTTGAGT                                   <  SRR4302533.6274016/94‑1 (MQ=60)

                                                             CCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGGTATAACAACGGGGTTGAGTA                                  >  SRR4302533.2959005/1‑94 (MQ=60)

                                                             CCAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGGTATAACAACGGGGTTGAGTA                                  <  SRR4302533.361728/94‑1 (MQ=60)

                                                              CAGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGGTATAACAACGGGGTTGAGTAT                                 >  SRR4302533.5886185/1‑94 (MQ=60)

                                                               AGTGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGGTATAACAACGGGGTTGAGTATT                                <  SRR4302533.329213/94‑1 (MQ=60)

                                                                 TGCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGGTATAACAACGGGGTTGAGTATTTA                              <  SRR4302533.288853/94‑1 (MQ=60)

                                                                  GCGCGACTCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGGTATAACAACGGGGTTGAGTATTTAT                             <  SRR4302533.3705499/94‑1 (MQ=60)

                                                                  GCGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGGTATAACAACGGGGTTGAGTATTTAT                             >  SRR4302533.2950905/1‑94 (MQ=60)

                                                                  GCGCCACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAGCCCTTCAGGTATAACAACGGGGTTGAGTATTTAT                             <  SRR4302533.3705500/94‑1 (MQ=60)

                                                                   CGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAATCCCTTCAGGTATAACAACGGGGTTGAGTATTTATC                            <  SRR4302533.2863982/94‑1 (MQ=60)

                                                                   CGCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGGTATAACAACGGGGTTGAGTATTTATC                            <  SRR4302533.2863983/94‑1 (MQ=60)

                                                                    GCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGGTATAACAACGGGGTTGAGTATTTATCG                           <  SRR4302533.3275319/94‑1 (MQ=60)

                                                                    GCGACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGGTATAACAACGGGGTTGAGTATTTATCG                           <  SRR4302533.73736/94‑1 (MQ=60)

                                                                      GACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGGTATAACAACGGGGTTGAGTATTTATCGAC                         <  SRR4302533.3550136/94‑1 (MQ=60)

                                                                      GACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGGTATAACAACGGGGTTGAGTATTTATCGAC                         <  SRR4302533.6207585/94‑1 (MQ=60)

                                                                      GACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGGTATAACAACGGGGTTGAGTATTTATCGAC                         >  SRR4302533.2590694/1‑94 (MQ=60)

                                                                       ACGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGGTATAACAACGGGGTTGAGTATTTATCGACT                        <  SRR4302533.651270/94‑1 (MQ=60)

                                                                        CGCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGGTATAACAACGGGGTTGAGTATGTATCGACTG                       >  SRR4302533.386871/1‑94 (MQ=60)

                                                                         GCCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGGTATAACAACGGGGTTGAGTATTTATCGACTGC                      <  SRR4302533.707397/94‑1 (MQ=60)

                                                                          CCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGGTATAACAACGGGGTTGAGTATTTATCGACTGCC                     >  SRR4302533.1507164/1‑94 (MQ=60)

                                                                          CCCCTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGGTATAACAACGGGGGTGAGTATTTATCGACTGCC                     >  SRR4302533.2096574/1‑94 (MQ=60)

                                                                             CTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGGTATAACAACGGGGTTGAGTATTTATCGACTGCCGGG                  <  SRR4302533.5897544/94‑1 (MQ=60)

                                                                             CTGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAAACCTTCAGGTATAACAACGGGGTTGAGTATTTATCGACTGCCGGG                  >  SRR4302533.2767681/1‑94 (MQ=60)

                                                                             CTGATTCCACAACCAGCGACCGATGCGACAGGCCTGCAAGGCATGAAAACCCTTCAGGTATAACAACGGGGTTGAGTATTTATCGACTGCCGGG                  <  SRR4302533.1380016/94‑1 (MQ=60)

                                                                              TGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGGTATAACAACGGGGTTGAGTATTTATCGACTGCCGGGT                 <  SRR4302533.1129467/94‑1 (MQ=60)

                                                                              TGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGGTATAACAACGGGGTTGAGTATTTATCGACTGCCGGGT                 <  SRR4302533.2482338/94‑1 (MQ=60)

                                                                              TGATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGGTATAACAACGGGGTTGAGTATTTATCGACTGCCGGGT                 <  SRR4302533.1668639/94‑1 (MQ=60)

                                                                              TGATTCCACAACCAGCGACCGATGAGATAGGCCTGCAAGGCATGAAAACCCTTCAGGTATAACAACGGGGTTGAGTATTTATCGACTGCCGGGT                 >  SRR4302533.1568910/1‑94 (MQ=60)

                                                                                ATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGGTATAACAACGGGGTTGAGTATTTATCGACTGCCGGGTCG               >  SRR4302533.2046846/1‑94 (MQ=60)

                                                                                ATTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGGTATAACAACGGGGATGAGTATTTATCGACTGCCGGGTCG               >  SRR4302533.1495458/1‑94 (MQ=60)

                                                                                 TTCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGGTATAACAACGGGGTTGAGTATTTATCGACTGCCGGGTCGC              <  SRR4302533.806215/94‑1 (MQ=60)

                                                                                  TCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAATCCCTTCAGGTATAACAACGGGGTTGAGTATTTATCGACTGCCGGGTCGCG             <  SRR4302533.4401109/94‑1 (MQ=60)

                                                                                  TCCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGGTATAACAACGGGGTTGAGTATTTATCGACTGCCGGGTCGCG             <  SRR4302533.6128474/94‑1 (MQ=60)

                                                                                   CCACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGGTATAACAACGGGGTTGAGTATTTATCGACTGCCGGGTCGCGG            >  SRR4302533.4416628/1‑94 (MQ=60)

                                                                                    CACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGGTATAACAACGGGGTTGAGTATTTATCGACTGCCGGGTCGCGGG           <  SRR4302533.2672578/94‑1 (MQ=60)

                                                                                    CACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGGTATAACAACGGGGTTGAGTATTTATCGACTGCCGGGTCGCGGG           <  SRR4302533.895201/94‑1 (MQ=60)

                                                                                    CACAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGGTATAACAACGGGGTTGAGTATTTATCGACTGCCGGGTCGCGGG           <  SRR4302533.2602882/94‑1 (MQ=60)

                                                                                      CAACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCGGGTATAACAACGGGGTTGAGTATTTATCGACTGCCGGGTCGCGGGTA         >  SRR4302533.99766/1‑94 (MQ=60)

                                                                                       AACCAGCGACCGATGCGATAGGCCTGCAAGTCATGAAAACCCTTCAGGTATAACAACGGGGTTGAGTATTTATCGACTGCCGGGTCGCGGGTAC        <  SRR4302533.1618960/94‑1 (MQ=60)

                                                                                       AACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGGTATAACAACGGGGTTGAGTATTTATCGACTGCCGGGTCGCGGGTAC        <  SRR4302533.4136098/94‑1 (MQ=60)

                                                                                       AACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGGTATAACAACGGGGTTGAGTATTTATCGACTGCCGGGTCGCGGGTAC        <  SRR4302533.4367994/94‑1 (MQ=60)

                                                                                       AACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGGTATAACAACGGGGTTGAGTATTTATCGACTGCCGGGTCGCGGGTAC        <  SRR4302533.3997485/94‑1 (MQ=60)

                                                                                        ACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGGTATAACAACGGGGTTGAGTATTTATCGACTGCCGGGTCGCGGGTACG       >  SRR4302533.1875787/1‑94 (MQ=60)

                                                                                        ACCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGGTATAACAACGGGGTTGAGTATTTATCGACTGCCGGGTCGCGGGTACG       <  SRR4302533.2769479/94‑1 (MQ=60)

                                                                                         CCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGGTATAACAACGGGGTTGAGTATTTATCGACTGCCGGGTCGCGGGTACGC      >  SRR4302533.1140069/1‑94 (MQ=60)

                                                                                         CCAGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGGTATAACAACGGGGTTGAGTATTTATCGACTGCCGGGTCGCGGGTACGC      <  SRR4302533.3090862/94‑1 (MQ=60)

                                                                                           AGCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGGTATAACAACGGGGTTGAGTATTTATCGACTGCCGGGTCGCGGGTACGCAC    <  SRR4302533.594849/94‑1 (MQ=60)

                                                                                            GCGACCGATGCGATAGGCCTGCAAGGCATGAAACCCCTTCAGGTATAACAACGGGGTTGAGTATTTATCGACTGCCGGGTCGCGGGTACGCACC   <  SRR4302533.1140316/94‑1 (MQ=60)

                                                                                            GCGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGGTATAACAACGGGGTTGAGTATTTATCGACTGCCGGGTCGCGGGTACGCACC   <  SRR4302533.1849659/94‑1 (MQ=60)

                                                                                             CGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGGTATAACAACGGGGTTGAGTATTTATCGACTGCCGGGTCGCGGGTACGCACCG  <  SRR4302533.3498674/94‑1 (MQ=60)

TTTGCTGCCGGGTGAATATCGACCTGGAACGTCACAGAAACCTGGTTTTGCAGAAAGATTGCCAGTGCGCGACGCCCCTGATTCCACAACCAGTGACCGATGCGATAGGCCTGCAAGGCATGAAAACCCTTCAGGTATAACAACGGGGTTGAGTATTTATCGACTGCCGGGTCGCGGGTACGCACCG  >  NC_000913/3779744‑3779930

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`