Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,837,057	G→A	L188L (CTC→CTT)	nlpA ←	lipoprotein‑28

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,837,057	0	G	A	100.0%	913.4 / NA	245	L188L (CTC→CTT)	nlpA	lipoprotein‑28
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (118/127); total (118/127)

Only 100 of 247 total aligned reads displayed.

GCTGTCGTGCACCGGAGAAAGCCCGGTCTGCTGAATGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGGAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAGCGCGGTAGGTAATAACCCTTTTCCCTCTTT  >  NC_000913/3836967‑3837146

gCTGTCGTGCACCGGAGAAAGCCCGGTCTGCTGAATGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAg                                                                                         <  1:4767784/93‑1 (MQ=255)

 cTGTCGTGCACCGGAGAAAGCCCGGTCTGCTGAATGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGc                                                                                        <  2:580782/93‑1 (MQ=255)

 cTGTCGTGCACCGGAGAAAGCCCGGTCTGCTGAATGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGc                                                                                        >  2:5980176/1‑93 (MQ=255)

 cTGTCGTGCACCGGAGAAAGCCCGGTCTGCTGAATGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGc                                                                                        <  2:1360466/93‑1 (MQ=255)

  tGTCGTGCACCGGAGAAAGCCCGGTCTGCTGAATGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCt                                                                                       >  1:100144/1‑93 (MQ=255)

   gTCGTGCACCGGAGAAAGCCCGGTCTGCTGAATGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTg                                                                                      >  2:1018424/1‑93 (MQ=255)

   gTCGTGCACCGGAGAAAGCCCGGTCTGCTGAATGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTg                                                                                      >  2:391884/1‑93 (MQ=255)

    tCGTGCACCGGAGAAAGCCCGGTCTGCTGAATGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTgc                                                                                     >  1:5263205/1‑93 (MQ=255)

     cGTGCACCGGAGAAAGCCCGGTCTGCTGAATGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTgcg                                                                                    >  1:1357019/1‑93 (MQ=255)

          aCCGGAGAAAGCCCGGTCTGCTGAATGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGccccc                                                                               >  2:5716920/1‑93 (MQ=255)

          aCCGGAGAAAGCCCGGTCTGCTGAATGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGccccc                                                                               >  1:2472555/1‑93 (MQ=255)

          aCCGGAGAAAGCCCGGTCTGCTGAATGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGccccc                                                                               >  1:1883773/1‑93 (MQ=255)

           ccGGAGAAAGCCCGGTCTGCTGAATGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCt                                                                              >  1:3927006/1‑93 (MQ=255)

            cGGAGAAAGCCCGGTCTGCTGAATGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTc                                                                             <  1:303559/93‑1 (MQ=255)

              gagaAAGCCCGGTCTGCTGAATGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGa                                                                           <  1:1929831/93‑1 (MQ=255)

               agaAAGCCCGGTCTGCTGAATGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAg                                                                          <  2:4784587/93‑1 (MQ=255)

               agaAAGCCCGGTCTGCTGAATGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAg                                                                          >  2:3141324/1‑93 (MQ=255)

               agaAAGCACGGTCTGCTGAAGGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAg                                                                          <  1:104757/93‑1 (MQ=255)

                gaAAGCCCGGTCTGCTGAATGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGt                                                                         <  1:4057444/93‑1 (MQ=255)

                 aaaGCCCGGTCTGCTGAATGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGtt                                                                        <  2:4666872/93‑1 (MQ=255)

                    gCCCGGTCTGCTGAATGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCa                                                                     >  1:3822607/1‑93 (MQ=255)

                     cccGGTCTGCTGAATGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCAt                                                                    <  2:3313504/93‑1 (MQ=255)

                      ccGGTCTGCTGAATGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATa                                                                   <  1:420266/93‑1 (MQ=255)

                         gTCTGCTGAATGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATc                                                                <  2:6288341/93‑1 (MQ=255)

                           ctgctgAATGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTg                                                              <  1:5426172/93‑1 (MQ=255)

                            tgctgAATGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGc                                                             >  2:2486680/1‑93 (MQ=255)

                             gctgAATGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCa                                                            >  1:4647385/1‑93 (MQ=255)

                              ctgAATGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCaa                                                           >  1:5783729/1‑93 (MQ=255)

                              ctgAATGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCaa                                                           >  1:781698/1‑93 (MQ=255)

                               tgAATGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCaaa                                                          >  1:4147994/1‑93 (MQ=255)

                                gAATGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAAt                                                         <  2:3489884/93‑1 (MQ=255)

                                 aaTGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATg                                                        <  2:1434396/93‑1 (MQ=255)

                                  aTGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGa                                                       <  1:5195121/93‑1 (MQ=255)

                                   tGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGAc                                                      <  1:4881661/93‑1 (MQ=255)

                                   tGTAAGTGGTACTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGAc                                                      <  1:3518613/93‑1 (MQ=255)

                                    gTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACg                                                     >  1:1763617/1‑93 (MQ=255)

                                    gTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACg                                                     >  2:2626356/1‑93 (MQ=255)

                                     tAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGa                                                    >  1:5450605/1‑93 (MQ=255)

                                       aGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAgg                                                  >  1:6192471/1‑93 (MQ=255)

                                        gtggtgCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGa                                                 >  2:3614867/1‑93 (MQ=255)

                                        gtggtgCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGa                                                 <  2:4520474/93‑1 (MQ=255)

                                          ggtgCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGAtt                                               <  2:2101279/93‑1 (MQ=255)

                                           gtgCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTa                                              >  1:5001232/1‑93 (MQ=255)

                                           gtgCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTa                                              <  2:456783/93‑1 (MQ=255)

                                           gtgCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTa                                              >  1:4852692/1‑93 (MQ=255)

                                             tcTGATAATCGCTACATCAACTTTGGGATCATCCAGTGCGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATc                                            <  1:5614191/92‑1 (MQ=255)

                                             gCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATc                                            <  2:3252101/93‑1 (MQ=255)

                                             gCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATc                                            >  1:3232740/1‑93 (MQ=255)

                                              cTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCg                                           <  2:5093720/93‑1 (MQ=255)

                                               tGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCgg                                          >  2:464597/1‑93 (MQ=255)

                                                 aTAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGtt                                        >  1:1204308/1‑93 (MQ=255)

                                                 aTAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGtt                                        <  2:482342/93‑1 (MQ=255)

                                                  tAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTta                                       <  2:3361399/93‑1 (MQ=255)

                                                    aTCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTtata                                     <  1:2936167/93‑1 (MQ=255)

                                                     tCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTatat                                    >  1:3049062/1‑93 (MQ=255)

                                                     tCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTACGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTatat                                    >  1:4429640/1‑93 (MQ=255)

                                                      cGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATc                                   >  2:5932150/1‑93 (MQ=255)

                                                       gCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGGTCCATAATCTGCAAATGACGAGGATTATCGGTTATATcc                                  >  1:3360155/1‑93 (MQ=255)

                                                        cTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCa                                 >  1:2844159/1‑93 (MQ=255)

                                                        cTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCa                                 <  1:3672588/93‑1 (MQ=255)

                                                        cTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCa                                 <  2:4337940/93‑1 (MQ=255)

                                                        cTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCa                                 >  1:302662/1‑93 (MQ=255)

                                                        cTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCa                                 <  1:5958570/93‑1 (MQ=255)

                                                         tACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAg                                <  2:2843789/93‑1 (MQ=255)

                                                         tACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAg                                <  1:1114409/93‑1 (MQ=255)

                                                           cATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAgcg                              >  2:2158209/1‑93 (MQ=255)

                                                           cATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAgcg                              >  2:4932806/1‑93 (MQ=255)

                                                             tCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAGcgcg                            <  2:3173711/93‑1 (MQ=255)

                                                              cAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAGCGCgg                           <  1:4407439/93‑1 (MQ=255)

                                                               aaCTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAGCGCggt                          >  1:6214491/1‑93 (MQ=255)

                                                               aaCTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAGCGCggt                          <  2:1564509/93‑1 (MQ=255)

                                                               aaCTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAGCGCgga                          >  2:776025/1‑92 (MQ=255)

                                                                 cTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAGCGCggtag                        >  2:838684/1‑93 (MQ=255)

                                                                 cTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAGCGCggtag                        <  1:5100950/93‑1 (MQ=255)

                                                                 cTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAGCGCggtag                        >  2:1735486/1‑93 (MQ=255)

                                                                  tttGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAGCGCggtagg                       >  2:3600093/1‑93 (MQ=255)

                                                                  tttGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAGCGCggtagg                       <  1:4508518/93‑1 (MQ=255)

                                                                   ttGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATGTCCAGCGCggtaggt                      <  2:1060011/93‑1 (MQ=255)

                                                                    tGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAGCGCggtaggta                     <  2:523289/93‑1 (MQ=255)

                                                                     gggATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAGCGCGGTAGGtaa                    <  2:3786428/93‑1 (MQ=255)

                                                                     gggATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAGCGCGGTAGGtaa                    >  2:2662512/1‑93 (MQ=255)

                                                                        atcatcCAGTACGCGCGTAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAGCGCGGTAGGtaataa                 <  1:6278917/93‑1 (MQ=255)

                                                                         tcatcCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAGCGCGGTAGGTAATAAc                >  1:3155920/1‑93 (MQ=255)

                                                                         tcatcCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAGCGCGGTAGGTAATAAc                <  2:5546131/93‑1 (MQ=255)

                                                                           atcCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAGCGCGGTAGGTAATAAccc              >  2:5596044/1‑93 (MQ=255)

                                                                           atcCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAGCGCGGTAGGTAATAAccc              <  2:6016124/93‑1 (MQ=255)

                                                                            tcCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAGCGCGGTAGGTAATAACCCt             >  2:4352540/1‑93 (MQ=255)

                                                                            tcCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAGCGCGGTAGGTAATAACCCt             >  1:4334151/1‑93 (MQ=255)

                                                                             ccAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAGCGCGGTAGGTAATAACCCtt            <  2:5402474/93‑1 (MQ=255)

                                                                              cAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAGCGCGGTAGGTAATAACCCttt           >  1:2255602/1‑93 (MQ=255)

                                                                              cAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAGCGCGGTAGGTAATAACCCttt           <  1:830622/93‑1 (MQ=255)

                                                                               aGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAGCGCGGTAGGTAATAACCCtttt          <  1:1863664/93‑1 (MQ=255)

                                                                               aGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAGCGCGGTAGGTAATAACCCtttt          >  1:4223815/1‑93 (MQ=255)

                                                                                gTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAGCGCGGTAGGTAATAACCCTTTTc         >  1:2112415/1‑93 (MQ=255)

                                                                                 tACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAGCGCGGTAGGTAATAACCCTTTTcc        <  1:2700124/93‑1 (MQ=255)

                                                                                 tACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAGCGCGGTAGGTAATAACCCTTTTcc        >  1:3739963/1‑93 (MQ=255)

                                                                                 tACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAGCGCGGTAGGTAATAACCCTTTTcc        <  1:4374509/93‑1 (MQ=255)

                                                                                 tACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAGCGCGGTAGGTAATAACCCTTTTcc        >  2:2395875/1‑93 (MQ=255)

                                                                                     cgcgGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAGCGCGGTAGGTAATAACCCTTTTCCctct    <  2:6067418/93‑1 (MQ=255)

                                                                                       cgGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAGCGCGGTAGGTAATAACCCTTTTCCCTCttt  >  1:6095265/1‑93 (MQ=255)

GCTGTCGTGCACCGGAGAAAGCCCGGTCTGCTGAATGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGGAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAGCGCGGTAGGTAATAACCCTTTTCCCTCTTT  >  NC_000913/3836967‑3837146

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TACGCTGTCGTGCACCGGAGAAAGCCCGGTCTGCTGAATGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGGAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAGCGCGGTAGGTAATAACCCTTTTCCCTCTTTCAG  >  NC_000913/3836964‑3837149

TACGCTGTCGTGCACCGGAGAAAGCCCGGTCTGCTGAATGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGCATCATCCAGTACGCGCGGA                                                                                              >  SRR4302533.2973277/1‑94 (MQ=60)

 ACGCTGTCGTGCACCGGAGAAAGCCCGGTCTGCTGAATGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAA                                                                                             >  SRR4302533.4383871/1‑94 (MQ=60)

  CGCTGTCGTGCACCGGAGAAAGCCCGGTCTGCTGAATGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAG                                                                                            <  SRR4302533.961246/94‑1 (MQ=60)

   GCTGTCGTGCACCGGAGAAAGCCCGGTCTGCTGAATGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGC                                                                                           <  SRR4302533.1105386/94‑1 (MQ=60)

   GCTGTCGTGCACCGGAGAAAGCCCGGTCTGCTGAATGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGC                                                                                           <  SRR4302533.4796686/94‑1 (MQ=60)

    CTGTCGTGCACCGGAGAAAGCCCGGTCTGCTGAATGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCT                                                                                          >  SRR4302533.100767/1‑94 (MQ=60)

    CTGTCGTGCACCGGAGAAAGCCCGGTCTGCTGAATGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCT                                                                                          >  SRR4302533.5019955/1‑94 (MQ=60)

    CTGTCGTGCACCGGAGAAAGCCCGGTCTGCTGAATGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCT                                                                                          >  SRR4302533.4478772/1‑94 (MQ=60)

     TGTCGTGCACCGGAGAAAGCCCGGTCTGCTGAATGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTG                                                                                         >  SRR4302533.795730/1‑94 (MQ=60)

     TGTCGTGCACCGGAGAAAGCCCGGTCTGCTGAATGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTG                                                                                         <  SRR4302533.5470777/94‑1 (MQ=60)

      GTCGTGCACCGGAGAAAGCCCGGTCTGCTGAATGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGC                                                                                        >  SRR4302533.5294798/1‑94 (MQ=60)

      GTCGTGCACCGGAGAAAGCCCGGTCTGCTGAATGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGC                                                                                        <  SRR4302533.3173954/94‑1 (MQ=60)

       TCGTGCACCGGAGAAAGCCCGGTCTGCTGAATGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCG                                                                                       <  SRR4302533.5493012/94‑1 (MQ=60)

       TCGTGCACCGGAGAAAGCCCGGTCTGCTGAATGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCG                                                                                       >  SRR4302533.1365174/1‑94 (MQ=60)

       TCGTGCACCGGAGAAAGCCCGGTCTGCTGAATGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCG                                                                                       <  SRR4302533.4043955/94‑1 (MQ=60)

         GTGCACCGGAGAAAGCCCGGTCTGCTGAATGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCC                                                                                     >  SRR4302533.1030923/1‑94 (MQ=60)

          TGCACCGGAGAAAGCCCGGTCTGCTGAATGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCC                                                                                    <  SRR4302533.4423343/94‑1 (MQ=60)

            CACCGGAGAAAGCCCGGTCTGCTGAATGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCC                                                                                  >  SRR4302533.1895279/1‑94 (MQ=60)

            CACCGGAGAAAGCCCGGTCTGCTGAATGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCC                                                                                  >  SRR4302533.2487802/1‑94 (MQ=60)

             ACCGGAGAAAGCCCGGTCTGCTGAATGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCT                                                                                 >  SRR4302533.3950979/1‑94 (MQ=60)

               CGGAGAAAGCCCGGTCTGCTGAATGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCG                                                                               <  SRR4302533.305413/94‑1 (MQ=60)

                GGAGAAAGCCCGGTCTGCTGAATGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGA                                                                              >  SRR4302533.4425790/1‑94 (MQ=60)

                 GAGAAAGCCCGGTCTGCTGAATGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAG                                                                             <  SRR4302533.1941604/94‑1 (MQ=60)

                  AGAAAGCCCGGTCTGCTGAATGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGT                                                                            <  SRR4302533.1601255/94‑1 (MQ=60)

                  AGAAAGCACGGTCTGCTGAAGGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGT                                                                            <  SRR4302533.105410/94‑1 (MQ=60)

                   GAAAGCCCGGTCTGCTGAATGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTT                                                                           <  SRR4302533.4082143/94‑1 (MQ=60)

                    AAAGCCCGGTCTGCTGAATGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTC                                                                          >  SRR4302533.416704/1‑94 (MQ=60)

                     AAGCCCGGTCTGCTGAATGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCC                                                                         <  SRR4302533.3964707/94‑1 (MQ=60)

                     AAGCCCGGTCTGCTGAATGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCC                                                                         <  SRR4302533.4599296/94‑1 (MQ=60)

                     AAGCCCGGTCTGCTGAATGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCC                                                                         >  SRR4302533.1135774/1‑94 (MQ=60)

                      AGCCCGGTCTGCTGAATGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCA                                                                        >  SRR4302533.3845977/1‑94 (MQ=60)

                      AGCCCGGTCTGCTGAATGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCA                                                                        >  SRR4302533.6180578/1‑94 (MQ=60)

                        CCCGGTCTGCTGAATGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATA                                                                      <  SRR4302533.2086739/94‑1 (MQ=60)

                        CCCGGTCTGCTGAATGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATA                                                                      >  SRR4302533.1184197/1‑94 (MQ=60)

                        CCCGGTCTGCTGAATGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATA                                                                      >  SRR4302533.3714109/1‑94 (MQ=60)

                         CCGGTCTGCTGAATGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAA                                                                     <  SRR4302533.422781/94‑1 (MQ=60)

                          CGGTCTGCTGAATGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAAT                                                                    >  SRR4302533.5031429/1‑94 (MQ=60)

                             TCTGCTGAATGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTG                                                                 <  SRR4302533.3047551/94‑1 (MQ=60)

                              CTGCTGAATGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATATTCTGC                                                                >  SRR4302533.2230508/1‑94 (MQ=60)

                              CTGCTGAATGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGC                                                                <  SRR4302533.4191734/94‑1 (MQ=60)

                              CTGCTGAATGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGC                                                                >  SRR4302533.4364085/1‑94 (MQ=60)

                              CTGCTGAATGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGC                                                                <  SRR4302533.1936582/94‑1 (MQ=60)

                              CTGCTGAATGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGC                                                                <  SRR4302533.5458851/94‑1 (MQ=60)

                               TGCTGAATGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCA                                                               >  SRR4302533.3316073/1‑94 (MQ=60)

                               TGCTGAATGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCA                                                               >  SRR4302533.4675546/1‑94 (MQ=60)

                               TGCTGAATGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCA                                                               >  SRR4302533.1884790/1‑94 (MQ=60)

                                GCTGAATGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAA                                                              >  SRR4302533.786300/1‑94 (MQ=60)

                                GCTGAATGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAA                                                              >  SRR4302533.5818660/1‑94 (MQ=60)

                                 CTGAATGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAA                                                             >  SRR4302533.4173125/1‑94 (MQ=60)

                                  TGAATGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAAT                                                            >  SRR4302533.5732120/1‑94 (MQ=60)

                                  TGAATGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAAT                                                            >  SRR4302533.2051463/1‑94 (MQ=60)

                                   GAATGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATG                                                           <  SRR4302533.5457453/94‑1 (MQ=60)

                                    AATGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCCAATGC                                                          >  SRR4302533.5946701/1‑94 (MQ=60)

                                    AATGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGA                                                          >  SRR4302533.5946702/1‑94 (MQ=60)

                                     ATGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGAC                                                         <  SRR4302533.5226453/94‑1 (MQ=60)

                                      TGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACG                                                        <  SRR4302533.4911236/94‑1 (MQ=60)

                                      TGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACG                                                        >  SRR4302533.1774356/1‑94 (MQ=60)

                                      TGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACG                                                        <  SRR4302533.2972669/94‑1 (MQ=60)

                                      TGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACG                                                        <  SRR4302533.5235010/94‑1 (MQ=60)

                                      TGTAAGTGGTACTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACG                                                        <  SRR4302533.3540305/94‑1 (MQ=60)

                                       GTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGA                                                       >  SRR4302533.5203166/1‑94 (MQ=60)

                                       GTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGA                                                       >  SRR4302533.5483439/1‑94 (MQ=60)

                                        TAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAG                                                      <  SRR4302533.4899056/94‑1 (MQ=60)

                                        TAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAG                                                      >  SRR4302533.6220211/1‑94 (MQ=60)

                                        TAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAG                                                      <  SRR4302533.4348301/94‑1 (MQ=60)

                                        TAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAG                                                      <  SRR4302533.2980783/94‑1 (MQ=60)

                                         AAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGG                                                     >  SRR4302533.6229632/1‑94 (MQ=60)

                                           GTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGAT                                                   <  SRR4302533.6270251/94‑1 (MQ=60)

                                           GTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGAT                                                   <  SRR4302533.3451011/94‑1 (MQ=60)

                                             GGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTA                                                 <  SRR4302533.6073708/94‑1 (MQ=60)

                                             GGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTA                                                 >  SRR4302533.5031548/1‑94 (MQ=60)

                                             GGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTA                                                 >  SRR4302533.4882099/1‑94 (MQ=60)

                                              GTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTAT                                                <  SRR4302533.5356846/94‑1 (MQ=60)

                                              GTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTAT                                                <  SRR4302533.2367484/94‑1 (MQ=60)

                                               TGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATC                                               >  SRR4302533.3252659/1‑94 (MQ=60)

                                                TCTGATAATCGCTACATCAACTTTGGGATCATCCAGTGCGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCG                                              <  SRR4302533.5648127/94‑1 (MQ=60)

                                                 CTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGG                                             <  SRR4302533.2591314/94‑1 (MQ=60)

                                                   GATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTT                                           >  SRR4302533.1211547/1‑94 (MQ=60)

                                                   GATAATCGCTACATCAACTTTGGGATCATCCAGTAAGCACGGAAGCCGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTT                                           <  SRR4302533.2059718/94‑1 (MQ=60)

                                                    ATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTA                                          >  SRR4302533.4228780/1‑94 (MQ=60)

                                                    ATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTA                                          <  SRR4302533.3800049/94‑1 (MQ=60)

                                                     TAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTAT                                         >  SRR4302533.859023/1‑94 (MQ=60)

                                                       ATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATAT                                       <  SRR4302533.2954117/94‑1 (MQ=60)

                                                       ATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATAT                                       >  SRR4302533.3067726/1‑94 (MQ=60)

                                                       ATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTACGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATAT                                       >  SRR4302533.4456419/1‑94 (MQ=60)

                                                         CGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCC                                     <  SRR4302533.3627823/94‑1 (MQ=60)

                                                         CGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCC                                     <  SRR4302533.2732287/94‑1 (MQ=60)

                                                         CGCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGGTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCC                                     >  SRR4302533.3380915/1‑94 (MQ=60)

                                                          GCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCA                                    >  SRR4302533.304512/1‑94 (MQ=60)

                                                          GCTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCA                                    >  SRR4302533.2861663/1‑94 (MQ=60)

                                                           CTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAG                                   <  SRR4302533.4646199/94‑1 (MQ=60)

                                                           CTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAG                                   <  SRR4302533.5994611/94‑1 (MQ=60)

                                                           CTACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAG                                   <  SRR4302533.3695158/94‑1 (MQ=60)

                                                            TACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAGC                                  <  SRR4302533.4258626/94‑1 (MQ=60)

                                                            TACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAGC                                  <  SRR4302533.1121016/94‑1 (MQ=60)

                                                             ACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAGCG                                 <  SRR4302533.2448931/94‑1 (MQ=60)

                                                             ACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAGCG                                 <  SRR4302533.4797301/94‑1 (MQ=60)

                                                             ACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAGCG                                 <  SRR4302533.4199762/94‑1 (MQ=60)

                                                             ACATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAGCG                                 <  SRR4302533.881161/94‑1 (MQ=60)

                                                              CATCAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAGCGC                                <  SRR4302533.1891157/94‑1 (MQ=60)

                                                                 CAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAGCGCGGT                             >  SRR4302533.6251757/1‑94 (MQ=60)

                                                                 CAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAGCGCGGT                             >  SRR4302533.4766614/1‑94 (MQ=60)

                                                                 CAACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAGCGCGGT                             <  SRR4302533.4434053/94‑1 (MQ=60)

                                                                   ACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAGCGCGGTAG                           <  SRR4302533.1223765/94‑1 (MQ=60)

                                                                   ACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAGCGCGGTAG                           <  SRR4302533.3329155/94‑1 (MQ=60)

                                                                   ACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAGCGCGGTAG                           <  SRR4302533.3066926/94‑1 (MQ=60)

                                                                   ACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAGCGCGGTAG                           <  SRR4302533.443440/94‑1 (MQ=60)

                                                                   ACTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAGCGCGGTAG                           >  SRR4302533.3595711/1‑94 (MQ=60)

                                                                    CTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAGCGCGGTAGG                          <  SRR4302533.5558366/94‑1 (MQ=60)

                                                                    CTTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAGCGCGGTAGG                          <  SRR4302533.5131763/94‑1 (MQ=60)

                                                                     TTTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAGCGCGGTAGGT                         <  SRR4302533.4535823/94‑1 (MQ=60)

                                                                      TTGGGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAGCGCGGTAGGTA                        >  SRR4302533.6340719/1‑94 (MQ=60)

                                                                         GGATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAGCGCGGTAGGTAATA                     >  SRR4302533.6319831/1‑94 (MQ=60)

                                                                           ATCATCCAGTACGCGCGTAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAGCGCGGTAGGTAATAAC                   <  SRR4302533.6316402/94‑1 (MQ=60)

                                                                           ATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAGCGCGGTAGGTAATAAC                   <  SRR4302533.6246581/94‑1 (MQ=60)

                                                                           ATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAGCGCGGTAGGTAATAAC                   >  SRR4302533.5160053/1‑94 (MQ=60)

                                                                           ATCATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAGCGCGGTAGGTAATAAC                   >  SRR4302533.3175322/1‑94 (MQ=60)

                                                                              ATCCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAGCGCGGTAGGTAATAACCCT                >  SRR4302533.4360265/1‑94 (MQ=60)

                                                                                CCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAGCGCGGTAGGTAATAACCCTTT              <  SRR4302533.1805825/94‑1 (MQ=60)

                                                                                CCAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAGCGCGGTAGGTAATAACCCTTT              >  SRR4302533.2269555/1‑94 (MQ=60)

                                                                                 CAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAGCGCGGTAGGTAATAACCCTTTT             >  SRR4302533.2791836/1‑94 (MQ=60)

                                                                                 CAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAGCGCGGTAGGTAATAACCCTTTT             <  SRR4302533.5107862/94‑1 (MQ=60)

                                                                                 CAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAGCGCGGTAGGTAATAACCCTTTT             <  SRR4302533.835490/94‑1 (MQ=60)

                                                                                 CAGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAGCGCGGTAGGTAATAACCCTTTT             >  SRR4302533.4249351/1‑94 (MQ=60)

                                                                                  AGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAGCGCGGTAGGTAATAACCCTTTTC            >  SRR4302533.2125445/1‑94 (MQ=60)

                                                                                  AGTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAGCGCGGTAGGTAATAACCCTTTTC            <  SRR4302533.1875033/94‑1 (MQ=60)

                                                                                   GTACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAGCGCGGTAGGTAATAACCCTTTTCC           >  SRR4302533.3762901/1‑94 (MQ=60)

                                                                                    TACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAGCGCGGTAGGTAATAACCCTTTTCCC          <  SRR4302533.2716690/94‑1 (MQ=60)

                                                                                    TACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAGCGCGGTAGGTAATAACCCTTTTCCC          <  SRR4302533.4400914/94‑1 (MQ=60)

                                                                                     ACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAGCGCGGTAGGTAATAACCCTTTTCCCT         <  SRR4302533.4834789/94‑1 (MQ=60)

                                                                                     ACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAGCGCGGTAGGTAATAACCCTTTTCCCT         <  SRR4302533.323437/94‑1 (MQ=60)

                                                                                     ACGCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAGCGCGGTAGGTAATAACCCTTTTCCCT         >  SRR4302533.171241/1‑94 (MQ=60)

                                                                                       GCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAGCGCGGTAGGTAATAACCCTTTTCCCTCT       <  SRR4302533.2865430/94‑1 (MQ=60)

                                                                                       GCGCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAGCGCGGTAGGTAATAACCCTTTTCCCTCT       <  SRR4302533.3838263/94‑1 (MQ=60)

                                                                                         GCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAGCGCGGTAGGTAATAACCCTTTTCCCTCTTT     >  SRR4302533.6132088/1‑94 (MQ=60)

                                                                                         GCGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAGCGCGGTAGGTAATAACCCTTTTCCCTCTTT     >  SRR4302533.3096996/1‑94 (MQ=60)

                                                                                          CGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAGCGCGGTAGGTAATAACCCTTTTCCCTCTTTC    >  SRR4302533.3114026/1‑94 (MQ=60)

                                                                                          CGGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAGCGCGGTAGGTAATAACCCTTTTCCCTCTTTC    <  SRR4302533.3901605/94‑1 (MQ=60)

                                                                                           GGAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAGCGCGGTAGGTAATAACCCTTTTCCCTCTTTCA   >  SRR4302533.5833191/1‑94 (MQ=60)

                                                                                            GAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAGCGCGGTAGGTAATAACCCTTTTCCCTCTTTCAG  <  SRR4302533.1290296/94‑1 (MQ=60)

                                                                                            GAAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAGCGCGGTAGGTAATAACCCTTTTCCCTCTTTCAG  >  SRR4302533.1688319/1‑94 (MQ=60)

                                                                                            GAAGCTGCGCCCCCTCGAGTGCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAGCGCGGTAGGTAATAACCCTTTTCCCTCTTTCAG  <  SRR4302533.1717708/94‑1 (MQ=60)

TACGCTGTCGTGCACCGGAGAAAGCCCGGTCTGCTGAATGTAAGTGGTGCTGATAATCGCTACATCAACTTTGGGATCATCCAGTACGCGCGGGAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAGCGCGGTAGGTAATAACCCTTTTCCCTCTTTCAG  >  NC_000913/3836964‑3837149

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`