Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,847,877	G→A	A159A (GCC→GCT)	uhpA ←	UhpA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,847,877	0	G	A	100.0%	880.1 / NA	235	A159A (GCC→GCT)	uhpA	UhpA
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (127/108); total (127/108)

Only 100 of 244 total aligned reads displayed.

CGCCAGCTCTACGTCGTTACTGACGCCCAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCGGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTGACGACCGGATGCCAG  >  NC_000913/3847787‑3847966

cgcCAGCTCTACGTCGTTACTGACGCCCAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCggcagc                                                                                         >  2:2039242/1‑93 (MQ=255)

cgcCAGCTCTACGTCGTTACTGACGCCCAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCggcagc                                                                                         >  2:4603860/1‑93 (MQ=255)

   cAGCTCTACGTCGTTACTGACGCCCAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAAt                                                                                      >  1:3342980/1‑93 (MQ=255)

    aGCTCTACGTCGTTACTGACGCCCAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAAtc                                                                                     >  1:2363644/1‑93 (MQ=255)

    aGCTCTACGTCGTTACTGACGCCCAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAAtc                                                                                     >  2:3539660/1‑93 (MQ=255)

     gCTCTACGTCGTTACTGACGCCCAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAAtct                                                                                    >  1:3823382/1‑93 (MQ=255)

     gCTCTACGTCGTTACTGACACCCAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAAtct                                                                                    >  2:6165247/1‑93 (MQ=255)

        ctACGTCGTTACTGACGCCCAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCtt                                                                                 <  1:701715/93‑1 (MQ=255)

        ctACGTCGTTACTGACGCCCAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCtt                                                                                 <  2:1732642/93‑1 (MQ=255)

          aCGTCGTTACTGACGCCCAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTc                                                                               >  2:3163289/1‑93 (MQ=255)

           cgtcgtTACTGACGCCCAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCa                                                                              <  1:1430242/93‑1 (MQ=255)

             tcgtTACTGACGCCCAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCAcc                                                                            >  1:5291017/1‑93 (MQ=255)

             tcgtTACTGACGCCCAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCAcc                                                                            >  1:5988252/1‑93 (MQ=255)

             tcgtTACTGACGCCCAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCAcc                                                                            >  1:5635666/1‑93 (MQ=255)

              cgtTACTGACGCCCAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCg                                                                           >  2:3767645/1‑93 (MQ=255)

               gtTACTGACGCCCAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGc                                                                          >  1:287342/1‑93 (MQ=255)

                ttACTGACGCCCAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGcc                                                                         >  1:5031500/1‑93 (MQ=255)

                ttACTGACGCCCAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGcc                                                                         >  1:3770071/1‑93 (MQ=255)

                  aCTGACGCCCAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCAt                                                                       >  1:1894190/1‑93 (MQ=255)

                    tGACGCCCAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTc                                                                     >  1:4244272/1‑93 (MQ=255)

                     gACGCCCAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTcc                                                                    >  1:1065392/1‑93 (MQ=255)

                      aCGCCCAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCt                                                                   <  2:2189880/93‑1 (MQ=255)

                       cGCCCAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCtt                                                                  >  2:14396/1‑93 (MQ=255)

                        gCCCAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTg                                                                 >  1:1500096/1‑93 (MQ=255)

                        gCCCAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTg                                                                 >  1:3501884/1‑93 (MQ=255)

                         cccAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTgc                                                                >  1:5939497/1‑93 (MQ=255)

                         cccAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTgc                                                                >  1:4689580/1‑93 (MQ=255)

                         cccAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTgc                                                                >  2:1131158/1‑93 (MQ=255)

                          ccAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTgcg                                                               >  1:2934732/1‑93 (MQ=255)

                           cAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTgcgc                                                              >  1:1248646/1‑93 (MQ=255)

                             gTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTgcgcta                                                            >  2:2616404/1‑91 (MQ=255)

                                tttCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGtt                                                         >  2:1504411/1‑93 (MQ=255)

                                   ccATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGttttt                                                      >  1:632849/1‑93 (MQ=255)

                                    cATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCCGTTTTTc                                                     >  2:4302913/1‑93 (MQ=255)

                                    cATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTc                                                     <  1:6278804/93‑1 (MQ=255)

                                    cATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTc                                                     >  1:4473537/1‑93 (MQ=255)

                                    cATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTc                                                     <  1:1715324/93‑1 (MQ=255)

                                     aTCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGttttttc                                                    >  2:101306/1‑91 (MQ=255)

                                     aTCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTcc                                                    >  1:4755533/1‑93 (MQ=255)

                                      tCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTccc                                                   >  2:30233/1‑92 (MQ=255)

                                      tCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCg                                                   >  2:86399/1‑93 (MQ=255)

                                      tCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCg                                                   >  2:5291944/1‑93 (MQ=255)

                                      tCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCg                                                   >  2:5051730/1‑93 (MQ=255)

                                      tCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCg                                                   >  1:3596660/1‑93 (MQ=255)

                                       cAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGc                                                  <  1:2444814/93‑1 (MQ=255)

                                           ttGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCAcc                                              >  2:781953/1‑93 (MQ=255)

                                            tGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCt                                             <  1:1301888/93‑1 (MQ=255)

                                             ggCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTg                                            <  2:2113065/93‑1 (MQ=255)

                                              gcgcGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTgg                                           >  1:509884/1‑93 (MQ=255)

                                                gcgATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCg                                         >  2:5347357/1‑93 (MQ=255)

                                                  gATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGtt                                       >  2:3676477/1‑93 (MQ=255)

                                                  gATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGtt                                       <  1:1171006/93‑1 (MQ=255)

                                                   aTGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTc                                      >  1:4194991/1‑93 (MQ=255)

                                                   aTGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTc                                      >  1:3268110/1‑93 (MQ=255)

                                                    tGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCa                                     >  2:2236093/1‑93 (MQ=255)

                                                    tGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCa                                     <  1:542713/93‑1 (MQ=255)

                                                    tGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCa                                     <  1:1011/93‑1 (MQ=255)

                                                     ggACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCAc                                    <  2:723782/93‑1 (MQ=255)

                                                      gACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACg                                   >  1:3175803/1‑93 (MQ=255)

                                                       aCGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGt                                  >  2:2178633/1‑93 (MQ=255)

                                                        cGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGtt                                 <  2:5139679/93‑1 (MQ=255)

                                                        cGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGtt                                 >  1:86513/1‑93 (MQ=255)

                                                         gtgtACCGTTTTCGGTGACATGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTTCGCCACCTGGCGTTCACGttt                                >  2:6067802/1‑93 (MQ=255)

                                                          tgtACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGTTTg                               >  1:2281939/1‑93 (MQ=255)

                                                           gtgccGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGTTTgg                              <  1:5471736/90‑1 (MQ=255)

                                                           gtACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGTTTgg                              >  2:1613425/1‑93 (MQ=255)

                                                            tACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGTTTGGt                             <  2:842435/93‑1 (MQ=255)

                                                            tACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGTTTGGt                             >  2:387203/1‑93 (MQ=255)

                                                            tACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGCTCACGTTTGGt                             >  1:5327049/1‑93 (MQ=255)

                                                             aCCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGTTTGGtt                            <  1:5518054/93‑1 (MQ=255)

                                                             aCCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGTTTGGtt                            >  2:4202284/1‑93 (MQ=255)

                                                              ccGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGTTTGGTTa                           <  2:1865050/93‑1 (MQ=255)

                                                              ccGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGTTTGGTTa                           <  1:1804444/93‑1 (MQ=255)

                                                               cGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGTTTGGTTAg                          >  2:2697303/1‑93 (MQ=255)

                                                                 ttttCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCg                        >  1:5249870/1‑93 (MQ=255)

                                                                  tttCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCAGCCACCTGGCGTTCACGTTTGGTTAGCgg                       >  1:2261669/1‑93 (MQ=255)

                                                                    tCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGt                     >  2:5881239/1‑93 (MQ=255)

                                                                      ggTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTcc                   <  2:1677892/93‑1 (MQ=255)

                                                                       gTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCt                  <  2:3420229/93‑1 (MQ=255)

                                                                        tGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTg                 <  1:214064/93‑1 (MQ=255)

                                                                          aCAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTGCTgac               >  1:4045365/1‑93 (MQ=255)

                                                                          aCAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTgac               <  2:2470125/93‑1 (MQ=255)

                                                                          aCAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTgac               <  1:588255/93‑1 (MQ=255)

                                                                            aaGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTgacga             >  1:2171748/1‑93 (MQ=255)

                                                                             aGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTgacgac            <  2:5788577/93‑1 (MQ=255)

                                                                             aGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTgacgac            <  1:2603242/93‑1 (MQ=255)

                                                                             aGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTgacgac            <  1:2694936/93‑1 (MQ=255)

                                                                              gCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTGACGAcc           <  1:6113634/93‑1 (MQ=255)

                                                                               cccAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTGACGACCg          <  2:3771730/93‑1 (MQ=255)

                                                                                ccAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTGACGACCgg         <  1:2525371/93‑1 (MQ=255)

                                                                                ccAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTGACGACCgg         >  1:3086364/1‑93 (MQ=255)

                                                                                 cAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTGACGACCGGa        >  2:282622/1‑93 (MQ=255)

                                                                                 cAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTGACGACCGGa        <  1:877262/93‑1 (MQ=255)

                                                                                 cAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTGACGACCGGa        >  1:3682855/1‑93 (MQ=255)

                                                                                 cAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTGACGACCGGa        <  1:3901172/93‑1 (MQ=255)

                                                                                   gTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTGACGACCGGATg      <  2:5430162/93‑1 (MQ=255)

                                                                                   gTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTGACGACCGGATg      >  2:1852455/1‑93 (MQ=255)

                                                                                     tCGGCACCAATCTCTTTCCCCGCCATTCCTTGCGCCAGTGTTTCCGCCACCTGGCTTTGTCGTTTGGTTAGCGTGTCCTGACGACCGGATGcc    <  2:3358997/93‑1 (MQ=255)

                                                                                      cggcagCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTGACGACCGGATGCCa   <  2:3378524/93‑1 (MQ=255)

                                                                                       ggcagcAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTGACGACCGGATGCCAg  <  2:4832848/93‑1 (MQ=255)

CGCCAGCTCTACGTCGTTACTGACGCCCAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCGGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTGACGACCGGATGCCAG  >  NC_000913/3847787‑3847966

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GCGCGCCAGCTCTACGTCGTTACTGACGCCCAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCGGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTGACGACCGGATGCCAGTTTA  >  NC_000913/3847784‑3847970

GCGCGCCAGCTCTACGTCGTTACTGACGCCCAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCA                                                                                               <  SRR4302533.4125408/94‑1 (MQ=60)

 CGCGCCAGCTCTACGTCGTTACTGACGCCCAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAG                                                                                              <  SRR4302533.4638087/94‑1 (MQ=60)

 CGCGCCAGCTCTACGTCGTTACTGACGCCCAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAG                                                                                              <  SRR4302533.5395742/94‑1 (MQ=60)

     CCAGCTCTACGTCGTTACTGACGCCCAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAAT                                                                                          >  SRR4302533.3363640/1‑94 (MQ=60)

      CAGCTCTACGTCGTTACTGACGCCCAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATC                                                                                         >  SRR4302533.324273/1‑94 (MQ=60)

      CAGCTCTACGTCGTTACTGACGCCCAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATC                                                                                         >  SRR4302533.2378265/1‑94 (MQ=60)

       AGCTCTACGTCGTTACTGACGCCCAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCT                                                                                        <  SRR4302533.5003339/94‑1 (MQ=60)

       AGCTCTACGTCGTTACTGACGCCCAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCT                                                                                        >  SRR4302533.3846759/1‑94 (MQ=60)

       AGCTCTACGTCGTTACTGACGCCCAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGCTCGGCAGCAATCT                                                                                        >  SRR4302533.6154176/1‑94 (MQ=60)

        GCTCTACGTCGTTACTGACGCCCAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTC                                                                                       <  SRR4302533.3732643/94‑1 (MQ=60)

           CTACGTCGTTACTGACGCCCAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTT                                                                                    <  SRR4302533.705868/94‑1 (MQ=60)

           CTACGTCGTTACTGACGCCCAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTT                                                                                    <  SRR4302533.1842350/94‑1 (MQ=60)

            TACGTCGTTACTGACGCCCAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTC                                                                                   <  SRR4302533.2482812/94‑1 (MQ=60)

             ACGTCGTTACTGACGCCCAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCA                                                                                  <  SRR4302533.945707/94‑1 (MQ=60)

             ACGTCGTTACTGACGCCCAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCA                                                                                  <  SRR4302533.1206015/94‑1 (MQ=60)

              CGTCGTTACTGACGCCCAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCAC                                                                                 <  SRR4302533.1417956/94‑1 (MQ=60)

              CGTCGTTACTGACGCCCAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCAC                                                                                 <  SRR4302533.1438886/94‑1 (MQ=60)

              CGTCGTTACTGACGCCCAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCAC                                                                                 >  SRR4302533.2051259/1‑94 (MQ=60)

               GTCGTTACTGACGCCCAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCANNTCGGCAGCAATCTTNTTCNCC                                                                                >  SRR4302533.2283405/1‑94 (MQ=60)

               GTCGTTACTGACGCCCAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACC                                                                                >  SRR4302533.5322699/1‑94 (MQ=60)

               GTCGTTACTGACGCCCAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACC                                                                                >  SRR4302533.492585/1‑94 (MQ=60)

               GTCGTTACTGACGCCCAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACC                                                                                >  SRR4302533.6024517/1‑94 (MQ=60)

               GTCGTTACTGACGCCCAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACC                                                                                >  SRR4302533.5669747/1‑94 (MQ=60)

               GTCGTTACTGACGCCCAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACC                                                                                >  SRR4302533.2338153/1‑94 (MQ=60)

                 CGTTACTGACGCCCAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGC                                                                              >  SRR4302533.2949061/1‑94 (MQ=60)

                 CGTTACTGACGCCCAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGC                                                                              >  SRR4302533.3957228/1‑94 (MQ=60)

                 CGTTACTGACGCCCAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGC                                                                              >  SRR4302533.289100/1‑94 (MQ=60)

                  GTTACTGACGCCCAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCC                                                                             <  SRR4302533.3590339/94‑1 (MQ=60)

                  GTTACTGACGCCCAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCC                                                                             >  SRR4302533.555892/1‑94 (MQ=60)

                  GTTACTGACGCCCAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCC                                                                             >  SRR4302533.3793184/1‑94 (MQ=60)

                  GTTACTGACGCCCAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCC                                                                             >  SRR4302533.5061965/1‑94 (MQ=60)

                  GTTACTGACGCCCAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCC                                                                             <  SRR4302533.3423308/94‑1 (MQ=60)

                   TTACTGACGCCCAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCA                                                                            >  SRR4302533.1647362/1‑94 (MQ=60)

                    TACTGACGCCCAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCAT                                                                           >  SRR4302533.1905753/1‑94 (MQ=60)

                      CTGACGCCCAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTC                                                                         >  SRR4302533.4269901/1‑94 (MQ=60)

                      CTGACGCCCAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTC                                                                         <  SRR4302533.5984160/94‑1 (MQ=60)

                       TGACGCCCAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCC                                                                        >  SRR4302533.1071650/1‑94 (MQ=60)

                       TGACGCCCAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCC                                                                        >  SRR4302533.2536733/1‑94 (MQ=60)

                       TGACGCCCAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCC                                                                        >  SRR4302533.1333120/1‑94 (MQ=60)

                         ACGCCCAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTT                                                                      <  SRR4302533.5138497/94‑1 (MQ=60)

                          CGCCCAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTG                                                                     >  SRR4302533.1509139/1‑94 (MQ=60)

                          CGCCCAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTG                                                                     >  SRR4302533.3523478/1‑94 (MQ=60)

                           GCCCAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGC                                                                    >  SRR4302533.4718010/1‑94 (MQ=60)

                           GCCCAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGC                                                                    >  SRR4302533.5975410/1‑94 (MQ=60)

                            CCCAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCG                                                                   >  SRR4302533.2952676/1‑94 (MQ=60)

                            CCCAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCG                                                                   <  SRR4302533.3073440/94‑1 (MQ=60)

                             CCAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGC                                                                  <  SRR4302533.2224035/94‑1 (MQ=60)

                             CCAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGC                                                                  >  SRR4302533.1256106/1‑94 (MQ=60)

                               AGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCA                                                                >  SRR4302533.4766543/1‑94 (MQ=60)

                                 TTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGT                                                              <  SRR4302533.1760486/94‑1 (MQ=60)

                                 TTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGT                                                              >  SRR4302533.3321340/1‑94 (MQ=60)

                                     TCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTT                                                          >  SRR4302533.636573/1‑94 (MQ=60)

                                     TCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTT                                                          <  SRR4302533.966168/94‑1 (MQ=60)

                                      CCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTC                                                         >  SRR4302533.4256332/1‑94 (MQ=60)

                                      CCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTC                                                         >  SRR4302533.4500594/1‑94 (MQ=60)

                                       CATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCC                                                        >  SRR4302533.4784390/1‑94 (MQ=60)

                                       CATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCC                                                        <  SRR4302533.3215298/94‑1 (MQ=60)

                                       CATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCC                                                        <  SRR4302533.1725724/94‑1 (MQ=60)

                                       CATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCC                                                        <  SRR4302533.6316289/94‑1 (MQ=60)

                                        ATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCG                                                       <  SRR4302533.1486388/94‑1 (MQ=60)

                                        ATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCG                                                       >  SRR4302533.1301018/1‑94 (MQ=60)

                                        ATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCG                                                       >  SRR4302533.6106351/1‑94 (MQ=60)

                                        ATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCG                                                       >  SRR4302533.3618791/1‑94 (MQ=60)

                                         TCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGC                                                      <  SRR4302533.1205300/94‑1 (MQ=60)

                                          CAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCC                                                     <  SRR4302533.2459905/94‑1 (MQ=60)

                                           AGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCA                                                    <  SRR4302533.3649755/94‑1 (MQ=60)

                                           AGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCA                                                    >  SRR4302533.5336495/1‑94 (MQ=60)

                                            GATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCAC                                                   <  SRR4302533.4610885/94‑1 (MQ=60)

                                             ATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACC                                                  <  SRR4302533.2874691/94‑1 (MQ=60)

                                             ATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACC                                                  <  SRR4302533.217226/94‑1 (MQ=60)

                                             ATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACC                                                  >  SRR4302533.5735007/1‑94 (MQ=60)

                                             ATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACC                                                  >  SRR4302533.5519309/1‑94 (MQ=60)

                                               tggccggattGACGTGTACCGTTTTCGGTGACAAGCCAGGTTCGGCAGCAATCTCTTTCACCGACATTCCTTGCGCCAGTTTTTCCGCCACCTG                                                <  SRR4302533.5532939/84‑1 (MQ=60)

                                               TGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTG                                                <  SRR4302533.3450778/94‑1 (MQ=60)

                                               TGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTG                                                <  SRR4302533.601357/94‑1 (MQ=60)

                                               TGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTG                                                <  SRR4302533.1309717/94‑1 (MQ=60)

                                                GGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGG                                               >  SRR4302533.512913/1‑94 (MQ=60)

                                                GGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGG                                               <  SRR4302533.2560802/94‑1 (MQ=60)

                                                 GCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGC                                              <  SRR4302533.2004996/94‑1 (MQ=60)

                                                    CGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTT                                           <  SRR4302533.2836121/94‑1 (MQ=60)

                                                     GATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTC                                          <  SRR4302533.1532143/94‑1 (MQ=60)

                                                     GATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTC                                          >  SRR4302533.4220383/1‑94 (MQ=60)

                                                     GATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTC                                          >  SRR4302533.6201275/1‑94 (MQ=60)

                                                     GATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTC                                          >  SRR4302533.4537181/1‑94 (MQ=60)

                                                     GATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTC                                          >  SRR4302533.3288276/1‑94 (MQ=60)

                                                     GATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTC                                          <  SRR4302533.1178036/94‑1 (MQ=60)

                                                      ATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCA                                         <  SRR4302533.4371458/94‑1 (MQ=60)

                                                       TGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCAC                                        <  SRR4302533.545934/94‑1 (MQ=60)

                                                       TGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCAC                                        <  SRR4302533.1020/94‑1 (MQ=60)

                                                       TGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCAC                                        <  SRR4302533.1131993/94‑1 (MQ=60)

                                                        GGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACG                                       >  SRR4302533.3195339/1‑94 (MQ=60)

                                                          ACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGTT                                     >  SRR4302533.87068/1‑94 (MQ=60)

                                                           CGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGTTT                                    <  SRR4302533.655224/94‑1 (MQ=60)

                                                            GTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGTTTG                                   >  SRR4302533.2296063/1‑94 (MQ=60)

                                                            GTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGTTTG                                   <  SRR4302533.5918429/94‑1 (MQ=60)

                                                             TGTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGTTTGT                                  >  SRR4302533.5420077/1‑94 (MQ=60)

                                                              GTGCCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGTTTGGT                                 <  SRR4302533.5504711/94‑1 (MQ=60)

                                                              GTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGTTTGGT                                 <  SRR4302533.877886/94‑1 (MQ=60)

                                                              GTACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGCTCACGTTTGGT                                 >  SRR4302533.5358972/1‑94 (MQ=60)

                                                               TACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGTTTGGTT                                >  SRR4302533.10273/1‑94 (MQ=60)

                                                               TACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGATTTTCCGCCACCTGGCGTTCACGTTTGGTT                                >  SRR4302533.4184271/1‑94 (MQ=60)

                                                                ACCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGTTTGGTTA                               <  SRR4302533.5551371/94‑1 (MQ=60)

                                                                 CCGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGTTTGGTTAG                              <  SRR4302533.1815428/94‑1 (MQ=60)

                                                                  CGTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGTTTGGTTAGC                             >  SRR4302533.3882848/1‑94 (MQ=60)

                                                                   GTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCG                            <  SRR4302533.710210/94‑1 (MQ=60)

                                                                   GTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCG                            >  SRR4302533.2335245/1‑94 (MQ=60)

                                                                   GTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCG                            <  SRR4302533.2984021/94‑1 (MQ=60)

                                                                   GTTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCG                            >  SRR4302533.5281422/1‑94 (MQ=60)

                                                                    TTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGG                           <  SRR4302533.534900/94‑1 (MQ=60)

                                                                    TTTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCAGCCACCTGGCGTTCACGTTTGGTTAGCGG                           >  SRR4302533.2275655/1‑94 (MQ=60)

                                                                     TTTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGG                          <  SRR4302533.5787982/94‑1 (MQ=60)

                                                                      TTCGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGT                         <  SRR4302533.5518643/94‑1 (MQ=60)

                                                                        CGGTGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCC                       >  SRR4302533.265265/1‑94 (MQ=60)

                                                                           TGACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTGA                    <  SRR4302533.215411/94‑1 (MQ=60)

                                                                            GACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTGCTGAC                   >  SRR4302533.4069985/1‑94 (MQ=60)

                                                                            GACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTGAC                   <  SRR4302533.4148151/94‑1 (MQ=60)

                                                                            GACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTGAC                   >  SRR4302533.5257076/1‑94 (MQ=60)

                                                                             ACAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTGACG                  <  SRR4302533.591728/94‑1 (MQ=60)

                                                                              CAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTGACGA                 >  SRR4302533.2185102/1‑94 (MQ=60)

                                                                              CAAGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTGACGA                 >  SRR4302533.5068889/1‑94 (MQ=60)

                                                                                AGCCCAGTTCGGCATCAATCTCTTTCACCGCCATTCCGTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTGACGACC               <  SRR4302533.857278/94‑1 (MQ=60)

                                                                                AGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTGACGACC               <  SRR4302533.2711451/94‑1 (MQ=60)

                                                                                AGCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTGACGACC               <  SRR4302533.2619236/94‑1 (MQ=60)

                                                                                 GCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTGACGACCG              <  SRR4302533.2264182/94‑1 (MQ=60)

                                                                                 GCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTGACGACCG              <  SRR4302533.6150527/94‑1 (MQ=60)

                                                                                 GCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTGACGACCG              >  SRR4302533.3140012/1‑94 (MQ=60)

                                                                                 GCCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTGACGACCG              <  SRR4302533.3715344/94‑1 (MQ=60)

                                                                                  CCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTGACGACCGG             >  SRR4302533.3105337/1‑94 (MQ=60)

                                                                                  CCCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTGACGACCGG             >  SRR4302533.5489629/1‑94 (MQ=60)

                                                                                   CCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTGACGACCGGA            >  SRR4302533.3705491/1‑94 (MQ=60)

                                                                                   CCAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTGACGACCGGA            <  SRR4302533.2540921/94‑1 (MQ=60)

                                                                                    CAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTGACGACCGGAT           >  SRR4302533.381942/1‑94 (MQ=60)

                                                                                    CAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTGACGACCGGAT           >  SRR4302533.633881/1‑94 (MQ=60)

                                                                                    CAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTGACGACCGGAT           >  SRR4302533.2406699/1‑94 (MQ=60)

                                                                                    CAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTGACGACCGGAT           <  SRR4302533.3924989/94‑1 (MQ=60)

                                                                                    CAGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTGACGACCGGAT           <  SRR4302533.882412/94‑1 (MQ=60)

                                                                                     AGTTCGGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTAGCGTTCACGTTTGGTTAGCGGGTCCTGACGACCGGATG          <  SRR4302533.5283880/94‑1 (MQ=60)

                                                                                          GGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTGACGACCGGATGCCAGT     <  SRR4302533.3890768/94‑1 (MQ=60)

                                                                                          GGCAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTGACGACCGGATGCCAGT     >  SRR4302533.4573562/1‑94 (MQ=60)

                                                                                            CAGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTGACGACCGGATGCCAGTTT   <  SRR4302533.5114422/94‑1 (MQ=60)

                                                                                             AGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTGACGACCGGATGCCAGTTTA  >  SRR4302533.5283756/1‑94 (MQ=60)

                                                                                             AGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTGACGACCGGATGCCAGTTTA  >  SRR4302533.2914980/1‑94 (MQ=60)

                                                                                             AGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTGACGACCGGATGCCAGTTTA  <  SRR4302533.3978286/94‑1 (MQ=60)

GCGCGCCAGCTCTACGTCGTTACTGACGCCCAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCGGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTGACGACCGGATGCCAGTTTA  >  NC_000913/3847784‑3847970

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`