Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,860,717 T→C T172A (ACG→GCG)  b4553 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,860,7170TC100.0% 703.6 / NA 220T172A (ACG→GCG) b4553hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (101/119);  total (101/119)

Only 100 of 234 total aligned reads displayed.

CAATCCCCATTTGTACTTTTGGCCAGCCGAGCAACGTCAGCCAGGCACAGGGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGTCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAGACGGTTATGCAGCGCCGTCACAATGCCGGAAATGATAATTGCGC  >  NC_000913/3860627‑3860806
                                                                                          |                                                                                         
 aaTCCCCATTTGTACTTTTGGCCAGCCGAGCAACGTCAGCCAGGCACAGGGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGcccc                                                                                        >  1:6024314/1‑93 (MQ=255)
  aTCCCCATTTGTACTTTTGGCCAGCCGAGCAACGTCAGCCAGGCACAGGGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGCcccc                                                                                       >  2:3718018/1‑93 (MQ=255)
  aTCCCCATTTGTACTTTTGGCCAGCCGAGCAACGTCAGCCAGGCACAGGGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGCcccc                                                                                       <  1:1234151/93‑1 (MQ=255)
    ccccATTTGTACTTTTGGCCAGCCGAGCAACGTCAGCCAGGCACAGGGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGCCCCCTg                                                                                     <  2:5885579/93‑1 (MQ=255)
     cccATTTGTACTTTTGGCCAGCCGAGCAACGTCAGCCAGGCACAGGGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGCCCCCTgg                                                                                    >  2:4222423/1‑93 (MQ=255)
     cccATTTGTACTTTTGGCCAGCCGAGCAACGTCAGCCAGGCACAGGGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGCCCCCTgg                                                                                    <  1:2174151/93‑1 (MQ=255)
     cccATTTGTACTTTTGGCCAGCCGAGCAACGTCAGCCAGGCACAGGGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGCCCCCTgg                                                                                    >  1:3740706/1‑93 (MQ=255)
         tttGTTCTTTTGGCCAGCCGAGCAACGTCAGCCAGGCACAGGGAATCATCACCAGGAAGTCGATAATCACCACATAAGACGCCCCCTGGaaaa                                                                                <  2:1005878/93‑1 (MQ=255)
         tttGTACTTTTGGCCAGCCGAGCAACGTCAGCCAGGCACAGGGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGCCCCCTGGaaaa                                                                                <  1:3995169/93‑1 (MQ=255)
         tttGTACTTTGGCCCAGCCGAGCAACGTCAGCCAGGCGCAGGGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGCCCCCTGGaaaa                                                                                <  2:3920182/93‑1 (MQ=255)
             tACTTTTGGCCAGCCGAGCAACGTCAGCCAGGCACAGGGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGCCCCCTGGAAAATGcc                                                                            >  2:4501334/1‑93 (MQ=255)
             tACTTTTGGCCAGCCGAGCAACGTCAGCCAGGCACAGGGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGCCCCCTGGAAAATGcc                                                                            <  1:2586771/93‑1 (MQ=255)
              aCTTTTGGCCAGCCGAGCAACGTCAGCCAGGCACAGGGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGCCCCCTGGAAAATGCCg                                                                           >  1:2985258/1‑93 (MQ=255)
               cTTTTGGCCAGCCGAGCAACGTCAGCCAGGCACAGGGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGCCCCCTGGAAAATGCCga                                                                          <  2:5656958/93‑1 (MQ=255)
                ttttGTCCAGACGAGCAACGTCAGCCAGGCACAGGGGATCATCACCAGGAAGGCGATAATCACCGCATAAGACGCCCCCTGGAAAATGCCgag                                                                         <  2:1963871/93‑1 (MQ=255)
                 tttGGCCAGCCGAGCAACGTCAGCCAGGCACAGGGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGCCCCCTGGAAAATGCCgaga                                                                        <  1:4290355/93‑1 (MQ=255)
                     gCCAGCCGAGCAACGTCAGCCAGGCACAGGGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGaaaaa                                                                    <  1:2999906/93‑1 (MQ=255)
                     gCCAGCCGAGCAACGTCAGCCAGGCACAGGGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGaaaaa                                                                    >  2:1989509/1‑93 (MQ=255)
                      ccAGCCGAGCAACGTCAGCCAGGCACAGGGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGCCCCCTGGGAAATGCCGAGAAAAAc                                                                   >  2:3647443/1‑93 (MQ=255)
                      ccAGCCGAGCAACGTCAGCCAGGCACAGGGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAAc                                                                   <  1:3064031/93‑1 (MQ=255)
                        aGCCGAGCAACGTCAGCCAGGCACAGGGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCg                                                                 <  2:4331562/93‑1 (MQ=255)
                          ccGAGCAACGTCAGCCAGGCACAGGGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGCCCCCTGGAAAATGCTGAGAAAAACCGGc                                                               >  1:4029181/1‑93 (MQ=255)
                          ccGAGCAACGTCAGCCAGGCACAGGGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGc                                                               <  1:5962506/93‑1 (MQ=255)
                           cGAGCAACGTCAGCCAGGCACAGGGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCa                                                              <  1:3219169/93‑1 (MQ=255)
                            gAGCAACGTCAGCCAGGCACAGGGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAg                                                             <  2:5395577/93‑1 (MQ=255)
                            gAGCAACGTCAGCCAGGCACAGGGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAg                                                             >  1:4657958/1‑93 (MQ=255)
                            gAGCAACGTCAGCCAGGCACAGGGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAg                                                             >  1:5225618/1‑93 (MQ=255)
                             aGCAACGTCAGCCAGGCACAGGGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGt                                                            >  2:4564264/1‑93 (MQ=255)
                               cAACGTCAGCCAGGCACAGGGGATCATCACCAGGAAGGCGATACTCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGttt                                                          <  2:6212195/93‑1 (MQ=255)
                                aaCGTCAGCCAGGCACAGGGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGtttt                                                         >  1:3429937/1‑93 (MQ=255)
                                 aCGTCAGCCAGGCACAGGGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGttttt                                                        <  2:1250039/93‑1 (MQ=255)
                                 aCGTCAGCCAGGCACAGGGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGttttt                                                        >  1:979890/1‑93 (MQ=255)
                                 aCGTCAGCCAGGCACAGGGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGttttt                                                        >  1:4956766/1‑93 (MQ=255)
                                   gTCAGCCAGGCACAGGGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGttttttt                                                      >  2:2615872/1‑92 (MQ=255)
                                       gCCAGGCACAGGGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGa                                                  >  1:794206/1‑93 (MQ=255)
                                        ccAGGCACAGGGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGaa                                                 <  2:1269261/93‑1 (MQ=255)
                                         cAGGCACAGGGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAAc                                                >  1:5314999/1‑93 (MQ=255)
                                         cAGGCACAGGGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAAc                                                <  2:1701041/93‑1 (MQ=255)
                                         cAGGCACAGGGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAAc                                                <  2:2991314/93‑1 (MQ=255)
                                          aGGCACAGGGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACa                                               >  1:5256760/1‑93 (MQ=255)
                                          aGGCACAGGGGATCATCACCAGGAACGCGATAATCACCGCATAGGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACa                                               <  1:2303272/93‑1 (MQ=255)
                                            gCACAGGGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAGa                                             <  2:5495591/93‑1 (MQ=255)
                                             cacaGGGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAGAc                                            <  2:1588004/93‑1 (MQ=255)
                                             cacaGGGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAGAc                                            >  1:5633139/1‑93 (MQ=255)
                                             cacaGGGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAGAc                                            <  2:2199681/93‑1 (MQ=255)
                                             cacaGGGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAGAc                                            <  2:5704595/93‑1 (MQ=255)
                                              acaGGGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAAAAGACg                                           >  2:3983926/1‑93 (MQ=255)
                                                aGGGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAGACGGt                                         <  1:4469720/93‑1 (MQ=255)
                                                 ggggATCATCACCAGGAAGGCGATAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAGACGGtt                                        >  1:4511901/1‑93 (MQ=255)
                                                 ggggATCATCACCAGGAAGGCGATAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAGACGGtt                                        <  2:3274300/93‑1 (MQ=255)
                                                 ggggATCATCACCAGGAAGGCGATAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAGACGGtt                                        >  2:4997392/1‑93 (MQ=255)
                                                  gggATCATCACCAGGAAGGCGATAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAGACGGTTa                                       <  1:737181/93‑1 (MQ=255)
                                                     atcatcACCAGGAAGGCGATAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAGACGGTTATGc                                    <  2:5851930/93‑1 (MQ=255)
                                                     atcatcACCAGGAAGGCGATAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAGACGGTTATGc                                    <  2:510136/93‑1 (MQ=255)
                                                       catcaCCAGGAAGGCGATAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAGACGGTTATGCAg                                  <  2:624693/93‑1 (MQ=255)
                                                       catcaCCAGGAAGGCGATAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAGACGGTTATGCAg                                  >  1:1654428/1‑93 (MQ=255)
                                                       catcaCCAGGAAGGCGATAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAGACGGTTATGCAg                                  <  1:1469120/93‑1 (MQ=255)
                                                        atcaCCAGGAAGGCGATAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAGACGGTTATGCAgc                                 <  1:5549771/93‑1 (MQ=255)
                                                          caCCAGGAAGGCGATAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAGACGGTTATGCAgcgc                               >  2:45399/1‑93 (MQ=255)
                                                           aCCAGGAAGGCGATAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAGACGGTTATGCAGCGcc                              >  1:1884908/1‑93 (MQ=255)
                                                            ccAGGAAGGCGATAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAGACGGTTATGCAGCGCCg                             >  2:2344500/1‑93 (MQ=255)
                                                             cAGGAAGGCGATAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAGACGGTTATGCAGCGCCGt                            <  1:2234320/93‑1 (MQ=255)
                                                             cAGGAAGGCGATAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAGACGGTTATGCAGCGCCGt                            >  2:2820533/1‑93 (MQ=255)
                                                             cAGGAAGGCGATAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAGACGGTTATGAGGCGCCgc                            >  1:1980650/1‑92 (MQ=255)
                                                               ggAAGGCGATAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAGACGGTTATGCAGCGCCGTca                          >  1:55761/1‑93 (MQ=255)
                                                                 aaGGCGATAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAGACGGTTATGCAGCGCCGTcaca                        >  2:5770374/1‑93 (MQ=255)
                                                                  aGGCGATAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAGACGGTTATGCAGCGCCGTCACaa                       >  2:67/1‑93 (MQ=255)
                                                                  aGGCGATAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAGACGGTTATGCAGCGCCGTCACaa                       <  2:2762985/93‑1 (MQ=255)
                                                                  aGGCGATAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAGACGGTTATGCAGCGCCGTCACaa                       <  1:651209/93‑1 (MQ=255)
                                                                  aGGCGATAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAGACGGTTATGCAGCGCCGTCACaa                       >  1:3181126/1‑93 (MQ=255)
                                                                   ggCGATAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAGACGGTTATGCAGCGCCGTCACAAt                      >  2:2523857/1‑93 (MQ=255)
                                                                     cGATAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAGACGGTTATGCAGCGCCGTCACAATGc                    <  1:925975/93‑1 (MQ=255)
                                                                      gATAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAGACGGTTATGCAGCGCCGTCACAATGcc                   >  2:1184121/1‑93 (MQ=255)
                                                                        tAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAGACGGTTATGCAGCGCCGTCACAATGCCgg                 >  2:2239382/1‑93 (MQ=255)
                                                                        tAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAGACGGTTACGCAGCGCCGTCACAATGCCgg                 <  1:3261700/93‑1 (MQ=255)
                                                                         aaTCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAGACGGTTATGCAGCGCCGTCACAATGCCGGa                <  2:2453596/93‑1 (MQ=255)
                                                                          aTCACCACATAAGACGCCCGCTGGAAAATGCCGAGAACAACCGGCAGTTTTTTATCGACCAGACGGTTATGCAGCGCCGTCACAATGCCGGaa               <  1:2566170/93‑1 (MQ=255)
                                                                          aTCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAGACGGTTATGCAGCGCCGTCACAATGCCGGaa               <  1:2877429/93‑1 (MQ=255)
                                                                           tCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAGACGGTTATGCAGCGCCGTCACAATGCCGGaaa              >  2:3138676/1‑93 (MQ=255)
                                                                           tCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAGACGGTTATGCAGCGCCGTCACAATGCCGGaaa              >  1:1675699/1‑93 (MQ=255)
                                                                           tCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAGACGGTTATGCAGCGCCGTCACAATGCCGGaaa              <  1:3623663/93‑1 (MQ=255)
                                                                           tCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAGACGGTTATGCAGCGCCGTCACAATGCCGGaaa              <  1:5933639/93‑1 (MQ=255)
                                                                               cacaTACGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAGACGGTTATGCAGCGCGGTCACAATGCAGGAATTGAt          >  2:5226945/1‑93 (MQ=255)
                                                                               cacaTAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAGACGGTTATGCAGCGCCGTCACAATGCCGGAAATGAt          >  1:5150895/1‑93 (MQ=255)
                                                                               cacaTAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAGACGGTTATGCAGCGCCGTCACAATGCCGGAAATGAt          <  1:843497/93‑1 (MQ=255)
                                                                               cacaTAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAGACGGTTATGCAGCGCCGTCACAATGCCGGAAATGAt          >  2:3515931/1‑93 (MQ=255)
                                                                                acaTAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAGACGGTTATGCAGCGCCGTCACAATGCCGGAAATGATa         <  1:111942/93‑1 (MQ=255)
                                                                                acaTAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAGACGGTTATGCAGCGCCGTCACAATGCCGGAAATGATa         <  2:1842237/93‑1 (MQ=255)
                                                                                acaTAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAGACGGTTATGCAGCGCCGTCACAATGCCGGAAATGATa         <  1:1832554/93‑1 (MQ=255)
                                                                                 caTAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAGACGGTTATGCAGCGCCGTCACAATGCCGGAAATGATaa        >  2:35532/1‑93 (MQ=255)
                                                                                 caTAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAGACGGTTATGCAGCGCCGTCACAATGCCGGAAATGATaa        <  2:5990975/93‑1 (MQ=255)
                                                                                  aTAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAGACGGTTATGCAGCGCCGTCACAATGCCGGAAATGATAAt       >  2:400465/1‑93 (MQ=255)
                                                                                   tAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAGACGGTTATGCAGCGCCGTCACAATGCCGGAAATGATAAtt      <  2:3199011/93‑1 (MQ=255)
                                                                                   tAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAGACGGTTATGCAGCGCCGTCACAATGCCGGAAATGATAAtt      <  2:1958279/93‑1 (MQ=255)
                                                                                   tAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAGACGGTTATGCAGCGCCGTCACAATGCCGGAAATGATAAtt      >  2:112063/1‑93 (MQ=255)
                                                                                   tAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAGACGGTTATGCAGCGCCGTCACAATGCCGGAAATGATAAtt      <  2:6291598/93‑1 (MQ=255)
                                                                                    aaGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAGACGGTTATGCAGCGCCGTCACAATGCCGGAAATGATAATTg     <  1:5023809/93‑1 (MQ=255)
                                                                                    aaGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAGACGGTTATGCAGCGCCGTCACAATGCCGGAAATGATAATTg     <  1:1069441/93‑1 (MQ=255)
                                                                                     aGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAGACGGTTATGCAGCGCCGTCACAATGTCGGAAATGATAATTgc    >  2:815736/1‑93 (MQ=255)
                                                                                       aCGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAGACGGTTATGCAGCGCCGTCACAATGCCGGAAATGATAATTgcgc  <  2:4689551/93‑1 (MQ=255)
                                                                                          |                                                                                         
CAATCCCCATTTGTACTTTTGGCCAGCCGAGCAACGTCAGCCAGGCACAGGGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGTCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAGACGGTTATGCAGCGCCGTCACAATGCCGGAAATGATAATTGCGC  >  NC_000913/3860627‑3860806

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATTCAATCCCCATTTGTACTTTTGGCCAGCCGAGCAACGTCAGCCAGGCACAGGGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGTCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAGACGGTTATGCAGCGCCGTCACAATGCCGGAAATGATAATTGCGCCGAT  >  NC_000913/3860624‑3860810
                                                                                             |                                                                                             
ATTCAATCCCCATTTGTACTTTTGGCCAGCCGAGCAACGTCAGCCAGGCACAGGGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGC                                                                                               >  SRR4302533.3757119/1‑94 (MQ=60)
 TTCAATCCCCATTTGTACTTTTGGCCAGCCGAGCAACGTCAGCCAGGCACAGGGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGCC                                                                                              <  SRR4302533.5130190/94‑1 (MQ=60)
 TTCAATCCCCATTTGTACTTTTGGCCAGCCGAGCAACGTCAGCCAGGCACAGGGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGCC                                                                                              >  SRR4302533.3001954/1‑94 (MQ=60)
 TTCAATCCCCATTTGTACTTTTGGCCAGCCGAGCAACGTCAGCCAGGCACAGGGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGCC                                                                                              <  SRR4302533.6012385/94‑1 (MQ=60)
 TTCAATCCCCATTTGTACTTTTGGCCAGCCGAGCAACGTCAGCCAGGCACAGGGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGCC                                                                                              <  SRR4302533.2962225/94‑1 (MQ=60)
 TTCAATCCCCATTTGTACTTTTGGCCAGCCGAGCAACGTCAGCCAGGCACAGGGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGCC                                                                                              >  SRR4302533.583669/1‑94 (MQ=60)
   CAATCCCCATTTGTACTTTTGGCCAGCCGAGCAACGTCAGCCAGGCACAGGGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGCCCC                                                                                            >  SRR4302533.6060821/1‑94 (MQ=60)
   CAATCCCCATTTGTACTTTTGGCCAGCCGAGCAACGTCAGCCAGGCACAGGGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGCCCC                                                                                            >  SRR4302533.4731332/1‑94 (MQ=60)
    AATCCCCATTTGTACTTTTGGCCAGCCGAGCAACGTCAGCCAGGCACAGGGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGCCCCC                                                                                           <  SRR4302533.827753/94‑1 (MQ=60)
     ATCCCCATTTGTACTTTTGGCCAGCCGAGCAACGTCAGCCAGGCACAGGGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGCCCCCT                                                                                          >  SRR4302533.3289250/1‑94 (MQ=60)
     ATCCCCATTTGTACTTTTGGCCAGCCGAGCAACGTCAGCCAGGCACAGGGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGCCCCCT                                                                                          <  SRR4302533.1241531/94‑1 (MQ=60)
     ATCCCCATTTGTACTTTTGGCCAGCCGAGCAACGTCAGCCAGGCACAGGGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGCCCCCT                                                                                          <  SRR4302533.4708434/94‑1 (MQ=60)
      TCCCCATTTGTACTTTTGGCCAGCCGAGCAACGTCAGCCAGGCACAGGGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGCCCCCTG                                                                                         >  SRR4302533.3255890/1‑94 (MQ=60)
       CCCCATTTGTACTTTTGGCCAGCCGAGCAACGTCAGCCAGGCACAGGGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGCCCCCTGG                                                                                        >  SRR4302533.794174/1‑94 (MQ=60)
       CCCCATTTGTACTTTTGGCCAGCCGAGCAACGTCAGCCAGGCACAGGGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGCCCCCTGG                                                                                        >  SRR4302533.3763646/1‑94 (MQ=60)
       CCCCATTTGTACTTTTGGCCAGCCGAGCAACGTCAGCCAGGCACAGGGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGCCCCCTGG                                                                                        <  SRR4302533.2601959/94‑1 (MQ=60)
       CCCCATTTGTACTTTTGGCCAGCCGAGCAACGTCAGCCAGGCACAGGGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGCCCCCTGG                                                                                        <  SRR4302533.444532/94‑1 (MQ=60)
       CCCCATTTGTACTTTTGGCCAGCCGAGCAACGTCAGCCAGGCACAGGGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGCCCCCTGG                                                                                        <  SRR4302533.5254957/94‑1 (MQ=60)
        CCCATTTGTACTTTTGGCCAGCCGAGCAACGTCAGCCAGGCACAGGGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGCCCCCTGGA                                                                                       <  SRR4302533.2187548/94‑1 (MQ=60)
        CCCATTTGTACTTTTGGCCAGCCGAGCAACGTCAGCCAGGCACAGGGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGCCCCCTGGA                                                                                       >  SRR4302533.5678853/1‑94 (MQ=60)
          CATTTGTACTTTTGGCCAGCCGAGCAACGTCAGCCAGGCACAGGGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGCCCCCTGGAAA                                                                                     >  SRR4302533.5311572/1‑94 (MQ=60)
           ATTTGTACTTTTGGCCAGCCGAGCAACGTCAGCCAGGCACAGGGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGCCCCCTGGCAAA                                                                                    >  SRR4302533.4028192/1‑94 (MQ=60)
           ATTTGTACTTTTGGCCAGCCGAGCAACGTCAGCCAGGCACAGGGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGCCCCCTGGAAAA                                                                                    >  SRR4302533.5991295/1‑94 (MQ=60)
            TTTGTACTTTTGGCCAGCCGAGCAACGTCAGCCAGGCACAGGGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGCCCCCTGGAAAAT                                                                                   <  SRR4302533.4019494/94‑1 (MQ=60)
            TTTGTACTTTTGGCCAGCCGAGCAACGTCAGCCAGGCACAGGGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGCCCCCTGGAAAAT                                                                                   >  SRR4302533.1003376/1‑94 (MQ=60)
             TTGTACTTTTGGCCAGCCGAGCAACGTCAGCCAGGCACAGGGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGCCCCCTGGAAAATG                                                                                  >  SRR4302533.3382312/1‑94 (MQ=60)
                TACTTTTGGCCAGCCGAGCAACGTCAGCCAGGCACAGGGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGCCCCCTGGAAAATGCCG                                                                               >  SRR4302533.3003522/1‑94 (MQ=60)
                TACTTTTGGCCAGCCGAGCAACGTCAGCCAGGCACAGGGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGCCCCCTGGAAAATGCCG                                                                               <  SRR4302533.2602673/94‑1 (MQ=60)
                 ACTTTTGGCCAGCCGAGCAACGTCAGCCAGGCACAGGGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGCCCCCTGGAAAATGCCGA                                                                              <  SRR4302533.3550509/94‑1 (MQ=60)
                 ACTTTTGGCCAGCCGAGCAACGTCAGCCAGGCACAGGGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGCCCCCTGGAAAATGCCGA                                                                              >  SRR4302533.386912/1‑94 (MQ=60)
                  CTTTTGGCCAGCCGAGCAACGTCAGCCAGGCACAGGGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGCCCCCTGGAAAATGCCGAG                                                                             >  SRR4302533.5268469/1‑94 (MQ=60)
                  CTTTTGGCCAGCCGAGCAACGTCAGCCAGGCACAGGGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGCCCCCTGGAAAATGCCGAG                                                                             >  SRR4302533.5349829/1‑94 (MQ=60)
                   TTTTGGCCAGCCGAGCAACGTCAGCCAGGCACAGGGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGA                                                                            <  SRR4302533.869569/94‑1 (MQ=60)
                    TTTGGCCAGCCGAGCAACGTCAGCCAGGCACAGGGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGAA                                                                           <  SRR4302533.4316158/94‑1 (MQ=60)
                        GCCAGCCGAGCAACGTCAGCCAGGCACAGGGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAAC                                                                       >  SRR4302533.3190982/1‑94 (MQ=60)
                        GCCAGCCGAGCAACGTCAGCCAGGCACAGGGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAAC                                                                       <  SRR4302533.3018253/94‑1 (MQ=60)
                         CCAGCCGAGCAACGTCAGCCAGGCACAGGGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACC                                                                      <  SRR4302533.3082845/94‑1 (MQ=60)
                         CAAGCCGAGCAACGTCAGCCAGGCTCTGCGGTTCATCACAAGGAAGGCGATAATAACAACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACC                                                                      <  SRR4302533.3082846/94‑1 (MQ=60)
                          CAGCCGAGCAACGTCAGCCAGGCACAGGGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCG                                                                     <  SRR4302533.5663067/94‑1 (MQ=60)
                            GCCGAGCAACGTCAGCCAGGCACAGGGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGCCCCCTGGAAAATGCTGAGAAAAACCGGC                                                                   >  SRR4302533.4053703/1‑94 (MQ=60)
                             CCGAGCAACGTCAGCCAGGCACAGGGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCA                                                                  <  SRR4302533.5998577/94‑1 (MQ=60)
                             CCGAGCAACGTCAGCCAGGCACAGGGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCA                                                                  >  SRR4302533.3006712/1‑94 (MQ=60)
                              CGAGCAACGTCAGCCAGGCACAGGGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAG                                                                 <  SRR4302533.3239019/94‑1 (MQ=60)
                              CGAGCAACGTCAGCCAGGCACAGGGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAG                                                                 >  SRR4302533.4686183/1‑94 (MQ=60)
                              CGAGCAACGTCAGCCAGGCACAGGGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAG                                                                 >  SRR4302533.1219058/1‑94 (MQ=60)
                              CGAGCAACGTCAGCCAGGCACAGGGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAG                                                                 >  SRR4302533.5257063/1‑94 (MQ=60)
                               GAGCAACGTCAGCCAGGCACAGGGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGT                                                                <  SRR4302533.2759968/94‑1 (MQ=60)
                                AGCAACGTCAGCCAGGCACAGGGGATCATCACAAGGAAGGCGATAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTT                                                               >  SRR4302533.2254819/1‑94 (MQ=60)
                                  CAACGTCAGCCAGGCACAGGGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTT                                                             >  SRR4302533.3451127/1‑94 (MQ=60)
                                  CAACGTCAGCCAGGCACAGGGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTT                                                             >  SRR4302533.167646/1‑94 (MQ=60)
                                   AACGTCAGCCAGGCACAGGGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTT                                                            >  SRR4302533.985636/1‑94 (MQ=60)
                                   AACGTCAGCCAGGCACAGGGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTT                                                            >  SRR4302533.4986791/1‑94 (MQ=60)
                                   AACGTCAGCCAGGCACAGGGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTT                                                            >  SRR4302533.1334380/1‑94 (MQ=60)
                                        CAGCCAGGCACAGGGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCG                                                       <  SRR4302533.1496792/94‑1 (MQ=60)
                                        CAGCCAGGCACAGGGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCG                                                       >  SRR4302533.44664/1‑94 (MQ=60)
                                         AGCCAGGCACAGGGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGA                                                      >  SRR4302533.798878/1‑94 (MQ=60)
                                          GCCAGGCACAGGGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAA                                                     <  SRR4302533.3058888/94‑1 (MQ=60)
                                          GCCAGGCACAGGGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAA                                                     <  SRR4302533.2896180/94‑1 (MQ=60)
                                           CCAGGCACAGGGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAAC                                                    >  SRR4302533.5346850/1‑94 (MQ=60)
                                           CCAGGCACAGGGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAAC                                                    >  SRR4302533.83476/1‑94 (MQ=60)
                                           CCAGGCACAGGGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAAC                                                    >  SRR4302533.1339467/1‑94 (MQ=60)
                                            CAGGCACAGGGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACA                                                   >  SRR4302533.1725576/1‑94 (MQ=60)
                                            CAGGCACAGGGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACA                                                   >  SRR4302533.5288331/1‑94 (MQ=60)
                                             AGGCACAGGGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAG                                                  <  SRR4302533.623140/94‑1 (MQ=60)
                                             AGGCACAGGGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAG                                                  <  SRR4302533.2359114/94‑1 (MQ=60)
                                             AGGCACAGGGGATCATCACCAGGAACGCGATAATCACCGCATAGGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAG                                                  <  SRR4302533.2317541/94‑1 (MQ=60)
                                               GCACAGGGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAGAC                                                >  SRR4302533.5667204/1‑94 (MQ=60)
                                               GCACAGGGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAGAC                                                <  SRR4302533.573293/94‑1 (MQ=60)
                                                 ACAGGGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAGACGG                                              >  SRR4302533.1950667/1‑94 (MQ=60)
                                                 ACAGGGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAGACGG                                              <  SRR4302533.222835/94‑1 (MQ=60)
                                                   AGGGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAGACGGTT                                            <  SRR4302533.4496753/94‑1 (MQ=60)
                                                   AGGGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAGACGGTT                                            >  SRR4302533.4539222/1‑94 (MQ=60)
                                                    GGGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAGACGGTTA                                           <  SRR4302533.1854953/94‑1 (MQ=60)
                                                    GGGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAGACGGTTA                                           <  SRR4302533.1806397/94‑1 (MQ=60)
                                                     GGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAGACGGTTAT                                          <  SRR4302533.741521/94‑1 (MQ=60)
                                                     GGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAGACGGTTAT                                          <  SRR4302533.3197576/94‑1 (MQ=60)
                                                      GGATCATCACCAGGAAGGCGATAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAGACGGTTATG                                         <  SRR4302533.4597754/94‑1 (MQ=60)
                                                      GGATCATCACCAGGAAGGCGATAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAGACGGTTATG                                         <  SRR4302533.2131355/94‑1 (MQ=60)
                                                        ATCATCACCAGGAAGGCGATAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAGACGGTTATGCA                                       >  SRR4302533.4524220/1‑94 (MQ=60)
                                                         TCATCACCAGGAAGGCGATAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAGACGGTTATGCAG                                      >  SRR4302533.1664445/1‑94 (MQ=60)
                                                          CATCACCAGGAAGGCGATAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAGACGGTTATGCAGC                                     <  SRR4302533.1477977/94‑1 (MQ=60)
                                                           ATCACCAGGAAGGCGATAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAGACGGTTATGCAGCG                                    >  SRR4302533.695556/1‑94 (MQ=60)
                                                           ATCACCAGGAAGGCGATAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAGACGGTTATGCAGCG                                    <  SRR4302533.5583286/94‑1 (MQ=60)
                                                             CACCAGGAAGGCGATAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAGACGGTTATGCAGCGCC                                  >  SRR4302533.1896419/1‑94 (MQ=60)
                                                               CCAGGAAGGCGATAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAGACGGTTATGaggcgccgc                                >  SRR4302533.1992775/1‑85 (MQ=60)
                                                                CAGGAAGGCGATAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAGACGGTTATGCAGCGCCGTC                               <  SRR4302533.2248105/94‑1 (MQ=60)
                                                                 AGGAAGGCGATAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAGACGGTTATGCAGCGCCGTCA                              >  SRR4302533.56135/1‑94 (MQ=60)
                                                                 AGGAAGGCGATAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAGACGGTTATGCAGCGCCGTCA                              >  SRR4302533.369132/1‑94 (MQ=60)
                                                                  GGAAGGCGATAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAGACGGTTATGCAGCGCCGTCAC                             >  SRR4302533.4087009/1‑94 (MQ=60)
                                                                    AAGGCGATAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAGACGGTTATGCAGCGCCGTCACAA                           >  SRR4302533.3200696/1‑94 (MQ=60)
                                                                     AGGCGATAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAGACGGTTATGCAGCGCCGTCACAAT                          <  SRR4302533.3902666/94‑1 (MQ=60)
                                                                     AGGCGATAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAGACGGTTATGCAGCGCCGTCACAAT                          <  SRR4302533.655058/94‑1 (MQ=60)
                                                                       GCGATAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAGACGGTTATGCAGCGCCGTCACAATGC                        >  SRR4302533.6286152/1‑94 (MQ=60)
                                                                        CGATAATCACCACATAAGACGCCCCTTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAGACGGTTATGCAGCGCCGTCACAATGCC                       <  SRR4302533.1090649/94‑1 (MQ=60)
                                                                        CGATAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAGACGGTTATGCAGCGCCGTCACAATGCC                       <  SRR4302533.931409/94‑1 (MQ=60)
                                                                        CGATAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAGACGGTTATGCAGCGCCGTCACAATGCC                       <  SRR4302533.2027780/94‑1 (MQ=60)
                                                                         GATAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAGACGGTTATGCAGCGCCGTCACAATGCCG                      >  SRR4302533.2370665/1‑94 (MQ=60)
                                                                         GATAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAGACGGTTATGCAGCGCCGTCACAATGCCG                      <  SRR4302533.5605194/94‑1 (MQ=60)
                                                                         GATAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAGACGGTTATGCAGCGCCGTCACAATGCCG                      <  SRR4302533.323075/94‑1 (MQ=60)
                                                                           TAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAGACGGTTATGCAGCGCCGTCACAATGCCGGA                    <  SRR4302533.95996/94‑1 (MQ=60)
                                                                           TAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAGACGGTTATGCAGCGCCGTCACAATGCCGGA                    <  SRR4302533.600576/94‑1 (MQ=60)
                                                                           TAATCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAGACGGTTACGCAGCGCCGTCACAATGCCGGA                    <  SRR4302533.3281819/94‑1 (MQ=60)
                                                                             ATCACCACATAAGACGCCCGCTGGAAAATGCCGAGAACAACCGGCAGTTTTTTATCGACCAGACGGTTATGCAGCGCCGTCACAATGCCGGAAA                  <  SRR4302533.2581962/94‑1 (MQ=60)
                                                                             ATCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAGACGGTTATGCAGCGCCGTCACAATGCCGGAAA                  >  SRR4302533.1685845/1‑94 (MQ=60)
                                                                             ATCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAGACGGTTATGCAGCGCCGTCACAATGCCGGAAA                  <  SRR4302533.2895136/94‑1 (MQ=60)
                                                                              TCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAGACGGTTATGCAGCGCCGTCACAATGCCGGAAAT                 <  SRR4302533.5969510/94‑1 (MQ=60)
                                                                              TCACCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAGACGGTTATGCAGCGCCGTCACAATGCCGGAAAT                 <  SRR4302533.3645963/94‑1 (MQ=60)
                                                                                TCCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAGACGGTTATGCAGCGCCGTCACAATGCCGGAAATGA               <  SRR4302533.2943802/94‑1 (MQ=60)
                                                                                 CCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAGACGGTTATGCAGCGCCGTCACAATGCCGGAAATGAT              >  SRR4302533.5515838/1‑94 (MQ=60)
                                                                                 CCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAGACGGTTATGCAGCGCCGTCACAATGCCGGAAATGAT              <  SRR4302533.5248494/94‑1 (MQ=60)
                                                                                 CCACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAGACGGTTATGCAGCGCCGTCACAATGCCGGAAATGAT              >  SRR4302533.5182011/1‑94 (MQ=60)
                                                                                  CACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAGACGGTTATGCAGCGCCGTCACAATGCCGGAAATGATA             <  SRR4302533.848428/94‑1 (MQ=60)
                                                                                   ACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAGACGGTTATGCAGCGCCGTCACAATGCCGGAAATGATAA            <  SRR4302533.1843740/94‑1 (MQ=60)
                                                                                   ACATAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAGACGGTTATGCAGCGCCGTCACAATGCCGGAAATGATAA            <  SRR4302533.112648/94‑1 (MQ=60)
                                                                                      TAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAGACGGTTATGCAGCGCCGTCACAATGCCGGAAATGATAATTG         <  SRR4302533.5072560/94‑1 (MQ=60)
                                                                                      TAAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAGACGGTTATGCAGCGCCGTCACAATGCCGGAAATGATAATTG         <  SRR4302533.5001091/94‑1 (MQ=60)
                                                                                       AAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAGACGGTTATGCAGCGCCGTCACAATGCCGGAAATGATAATTGC        <  SRR4302533.1075739/94‑1 (MQ=60)
                                                                                       AAGACGCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAGACGGTTATGCAGCGCCGTCACAATGCCGGAAATGATAATTGC        <  SRR4302533.5054232/94‑1 (MQ=60)
                                                                                            GCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAGACGGTTATGCAGCGCCGTCACAATGCCGGAAATGATAATTGCGCCGA   >  SRR4302533.1295778/1‑94 (MQ=60)
                                                                                            GCCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAGACGGTTATGCAGCGCCGTCACAATGCCGGAAATGATAATTGCGCCGA   >  SRR4302533.2911699/1‑94 (MQ=60)
                                                                                             CCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAGACGGTTATGCAGCGCCGTCACAATGCCGGAAATGATAATTGCGCCGAT  <  SRR4302533.3293466/94‑1 (MQ=60)
                                                                                             CCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAGACGGTTATGCAGCGCCGTCACAATGCCGGAAATGATAATTGCGCCGAT  <  SRR4302533.4671391/94‑1 (MQ=60)
                                                                                             CCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAGACGGTTATGCAGCGCCGTCACAATGCCGGAAATGATAATTGCGCCGAT  >  SRR4302533.4819903/1‑94 (MQ=60)
                                                                                             CCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAGACGGTTATGCAGCGCCGTCACAATGCCGGAAATGATAATTGCGCCGAT  >  SRR4302533.1596490/1‑94 (MQ=60)
                                                                                             |                                                                                             
ATTCAATCCCCATTTGTACTTTTGGCCAGCCGAGCAACGTCAGCCAGGCACAGGGGATCATCACCAGGAAGGCGATAATCACCACATAAGACGTCCCCTGGAAAATGCCGAGAAAAACCGGCAGTTTTTTATCGAACAGACGGTTATGCAGCGCCGTCACAATGCCGGAAATGATAATTGCGCCGAT  >  NC_000913/3860624‑3860810

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑