Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,869,012 T→C I116V (ATT→GTT)  b3691 ← D‑galactonate transport

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,869,0120TC100.0% 844.7 / NA 261I116V (ATT→GTT) b3691D‑galactonate transport
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (114/147);  total (114/147)

Only 100 of 271 total aligned reads displayed.

TATAAAAACCAACGGCAGAAGCGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAATACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACTGACCAGCCAAATATCGCAA  >  NC_000913/3868922‑3869101
                                                                                          |                                                                                         
tataAAAACCAACGGCAGAAGCGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAacac                                                                                         <  1:4777375/93‑1 (MQ=255)
tataAAAACCAACGGCAGAAGCGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAacac                                                                                         >  2:2141777/1‑93 (MQ=255)
 ataAAAACCAACGGCAGAAGCGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACAcc                                                                                        <  1:3816642/93‑1 (MQ=255)
   aaaaaCCAACGGCCGAAGCGCGATCATGTTCCGGGAACCAGCTGGTCACCATCCCGTTATTGGTCAGGAAAGCAGGCGCTTCGAAAACACCgg                                                                                      >  2:2902455/1‑93 (MQ=255)
   aaaaaCCAACGGCAGAAGCGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCgg                                                                                      >  1:3101893/1‑93 (MQ=255)
   aaaaaCCAACGGCAGAAGCGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCgg                                                                                      >  1:2997941/1‑93 (MQ=255)
     aaaCCAACGGCAGAAGCGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGtt                                                                                    <  2:1111310/93‑1 (MQ=255)
      aaCCAACGGCAGAAGCGCGTTCATGTTCCGGGAACAAGCTGGTCACCATCCGGTTATTGGTCGGCAAAGCAGGCGCTTCGAAAACACCGGTTa                                                                                   >  2:1421638/1‑93 (MQ=255)
       aCCAACGGCAGAAGCGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTAt                                                                                  <  1:4985241/93‑1 (MQ=255)
       aCCAACGGCAGAAGCGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTAt                                                                                  >  2:4630542/1‑93 (MQ=255)
       aCCAACGGCAGAAGCGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTAt                                                                                  >  1:4399764/1‑93 (MQ=255)
        ccAACGGCAGAAGCGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATc                                                                                 >  1:4462370/1‑93 (MQ=255)
        ccAACGGCAGAAGCGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATc                                                                                 >  1:4999987/1‑93 (MQ=255)
        ccAACGGCAGAAGCGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATc                                                                                 <  2:1188623/93‑1 (MQ=255)
         cAACGGCAGAAGCGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATcg                                                                                <  2:5828197/93‑1 (MQ=255)
         cAACGGCAGAAGCGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATcg                                                                                >  1:6250788/1‑93 (MQ=255)
         cAACGGCAGAAGCGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATcg                                                                                >  2:653915/1‑93 (MQ=255)
           aCGGCAGAAGCGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATcgcg                                                                              >  2:1044056/1‑93 (MQ=255)
            cGGCAGAAGCGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATcgcgc                                                                             >  1:5972193/1‑93 (MQ=255)
             ggCAGAAGCGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATcgcgcg                                                                            >  2:506380/1‑93 (MQ=255)
               cAGAAGCGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCa                                                                          <  1:5646977/93‑1 (MQ=255)
                 gaggcgcGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGa                                                                        <  1:5318928/90‑1 (MQ=255)
                 gAAGCGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGa                                                                        >  2:4406173/1‑93 (MQ=255)
                  aaGCGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGAc                                                                       <  2:4276354/93‑1 (MQ=255)
                  aaGCGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGAc                                                                       <  1:5067306/93‑1 (MQ=255)
                   aGCGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGAcc                                                                      <  2:1998843/93‑1 (MQ=255)
                   aGCGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGAcc                                                                      <  2:183242/93‑1 (MQ=255)
                   aGCGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGAcc                                                                      >  2:4936428/1‑93 (MQ=255)
                     cgcgTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCaa                                                                    <  2:6233000/93‑1 (MQ=255)
                         ttCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTaa                                                                >  1:2630844/1‑93 (MQ=255)
                           cATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATg                                                              >  1:845835/1‑93 (MQ=255)
                             tGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACTCCGGTTATCGCGCGCAGACCAATTAATGAc                                                            <  2:4830127/93‑1 (MQ=255)
                             tGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGAc                                                            <  1:4967329/93‑1 (MQ=255)
                             tGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACAACGGTTATCGCGCGCAGACCAATTAATGAc                                                            >  1:1485419/1‑93 (MQ=255)
                              gTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACa                                                           <  2:5866377/93‑1 (MQ=255)
                              gTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACa                                                           >  2:2346302/1‑93 (MQ=255)
                              gTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACa                                                           >  2:712593/1‑93 (MQ=255)
                                 ccGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTa                                                        <  2:3804244/93‑1 (MQ=255)
                                 ccGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTa                                                        <  1:2414133/93‑1 (MQ=255)
                                 ccGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTa                                                        <  2:1989732/93‑1 (MQ=255)
                                 ccGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTa                                                        <  1:4507906/93‑1 (MQ=255)
                                  cGGGATCCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTaa                                                       <  2:4010427/93‑1 (MQ=255)
                                  cGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTaa                                                       <  2:3025539/93‑1 (MQ=255)
                                   gggAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAg                                                      >  1:3235469/1‑93 (MQ=255)
                                    ggAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGc                                                     <  2:430780/93‑1 (MQ=255)
                                    ggAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGc                                                     <  2:759731/93‑1 (MQ=255)
                                    ggAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGc                                                     >  2:2413552/1‑93 (MQ=255)
                                      aaCCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGccc                                                   <  1:22104/93‑1 (MQ=255)
                                      aaCCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGccc                                                   <  1:4891056/93‑1 (MQ=255)
                                       aCCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCg                                                  >  1:203076/1‑93 (MQ=255)
                                       aCCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCg                                                  >  1:416505/1‑93 (MQ=255)
                                       aCCAGATGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGAAGACCAATTAATGACATTAAGCCCg                                                  >  2:3381290/1‑93 (MQ=255)
                                        ccAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGt                                                 <  2:3082038/93‑1 (MQ=255)
                                        ccAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGt                                                 <  1:3849501/93‑1 (MQ=255)
                                         cAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTg                                                <  2:1263803/93‑1 (MQ=255)
                                          aGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTgg                                               <  1:2735216/93‑1 (MQ=255)
                                          aGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTgg                                               >  2:3673682/1‑93 (MQ=255)
                                           gCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGc                                              <  2:866961/93‑1 (MQ=255)
                                           gCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGc                                              <  1:5779370/93‑1 (MQ=255)
                                             tGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCaa                                            >  2:1573183/1‑93 (MQ=255)
                                             tGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCG‑AAACAACGGTTATCGCGCGCCGGCCAATTAATGACATTAAGACCGTGGCaaa                                           >  2:5386321/1‑93 (MQ=255)
                                              ggTCACCCTCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCaaa                                           <  1:3324392/93‑1 (MQ=255)
                                                  aCCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGccc                                       >  2:5342305/1‑93 (MQ=255)
                                                    cATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTg                                     <  2:3588657/93‑1 (MQ=255)
                                                    cATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTg                                     <  1:6157807/93‑1 (MQ=255)
                                                      tCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGa                                   >  1:174071/1‑93 (MQ=255)
                                                      tCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGa                                   <  1:5447169/93‑1 (MQ=255)
                                                      tCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGa                                   >  1:2280046/1‑93 (MQ=255)
                                                      tCCGGTTATTCGCCGGGAAAGCAGGCGCCTCGAAAAAACGGGTTGTAGCGCGCAGACCAATTCATAACATTAAGCTCGAGGCACAGCCCTGGa                                   >  2:5964783/1‑93 (MQ=255)
                                                          gTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATaa                               >  1:1888083/1‑93 (MQ=255)
                                                          gTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATaa                               >  2:5679953/1‑93 (MQ=255)
                                                           ttATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATaaa                              <  2:3924502/93‑1 (MQ=255)
                                                           ttATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATaaa                              <  2:5277180/93‑1 (MQ=255)
                                                             aTTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGt                            <  1:346540/93‑1 (MQ=255)
                                                              ttGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTc                           >  2:5651787/1‑93 (MQ=255)
                                                               tGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCg                          >  2:6002414/1‑93 (MQ=255)
                                                                 gTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGcc                        >  2:2829497/1‑93 (MQ=255)
                                                                 gTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGcc                        <  1:6021074/93‑1 (MQ=255)
                                                                  tCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCa                       >  2:5604539/1‑93 (MQ=255)
                                                                  tCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCa                       <  1:1844659/93‑1 (MQ=255)
                                                                  tCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCa                       >  1:1755075/1‑93 (MQ=255)
                                                                    gggAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTTGCCACt                     >  2:5217670/1‑93 (MQ=255)
                                                                    gggAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACt                     >  1:3037917/1‑93 (MQ=255)
                                                                    gggAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACt                     >  1:5687912/1‑93 (MQ=255)
                                                                     ggAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACTg                    >  1:5944398/1‑93 (MQ=255)
                                                                      gAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACTGa                   <  2:3434239/93‑1 (MQ=255)
                                                                       aaaGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACTGAc                  <  1:1179963/93‑1 (MQ=255)
                                                                        aaGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACTGAcc                 <  1:4366457/93‑1 (MQ=255)
                                                                        aaGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACTGAcc                 <  1:2874697/93‑1 (MQ=255)
                                                                         aGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACTGACCa                <  2:1695224/93‑1 (MQ=255)
                                                                         aGCAGGCGCTTCGAAAACACCGGTCATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACTGACCa                <  2:3882545/93‑1 (MQ=255)
                                                                           cAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACTGACCAGc              >  1:4721487/1‑93 (MQ=255)
                                                                            aGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACTGACCAGcc             >  2:2245473/1‑93 (MQ=255)
                                                                                gcTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACTGACCAGCCAAat         >  2:6081080/1‑93 (MQ=255)
                                                                                 cTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACTGACCAGCCAAata        <  1:5360524/93‑1 (MQ=255)
                                                                                 cTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACTGACCAGCCAAata        >  2:4136341/1‑93 (MQ=255)
                                                                                  ttCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACTGACCAGCCAAatat       >  1:3792997/1‑93 (MQ=255)
                                                                                   tCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACTGACCAGCCAAATATc      <  2:293766/93‑1 (MQ=255)
                                                                                      aaaaCACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACTGACCAGCCAAATATCGCa   >  1:984986/1‑93 (MQ=255)
                                                                                       aaaCACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACTGACCAGCCAAATATCGCaa  <  2:6288474/93‑1 (MQ=255)
                                                                                          |                                                                                         
TATAAAAACCAACGGCAGAAGCGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAATACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACTGACCAGCCAAATATCGCAA  >  NC_000913/3868922‑3869101

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ACGTATAAAAACCAACGGCAGAAGCGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAATACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACTGACCAGCCAAATATCGCAATAAA  >  NC_000913/3868919‑3869105
                                                                                             |                                                                                             
ACGTATAAAAACCAACGGCAGAAGCGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAAC                                                                                               >  SRR4302533.4838892/1‑94 (MQ=60)
 CGTATAAAAACCAACGGCAGAAGCGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACA                                                                                              >  SRR4302533.5418046/1‑94 (MQ=60)
 CGTATAAAAACCAACGGCAGAAGCGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACA                                                                                              <  SRR4302533.2118651/94‑1 (MQ=60)
   TATAAAAACCAACGGCAGAAGCGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACC                                                                                            >  SRR4302533.5758777/1‑94 (MQ=60)
   TATAAAAACCAACGGCAGAAGCGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACC                                                                                            <  SRR4302533.4806312/94‑1 (MQ=60)
    ATAAAAACCAACGGCAGAAGCGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCG                                                                                           <  SRR4302533.3965231/94‑1 (MQ=60)
    ATAAAAACCAACGGCAGAAGCGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCG                                                                                           <  SRR4302533.3839978/94‑1 (MQ=60)
     TAAAAACCAACGGCAGAAGCGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGG                                                                                          >  SRR4302533.3120964/1‑94 (MQ=60)
     TAAAAACCAACGGCAGAAGCGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGG                                                                                          >  SRR4302533.3016271/1‑94 (MQ=60)
      AAAAACCAACGGCAGAAGCGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGT                                                                                         <  SRR4302533.5469331/94‑1 (MQ=60)
      AAAAACCAACGGCAGAAGCGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGT                                                                                         >  SRR4302533.620464/1‑94 (MQ=60)
       AAAACCAACGGCAGAAGCGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTT                                                                                        <  SRR4302533.379115/94‑1 (MQ=60)
       AAAACCAACGGCAGAAGCGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTT                                                                                        >  SRR4302533.3091548/1‑94 (MQ=60)
        AAACCAACGGCAGAAGCGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTA                                                                                       <  SRR4302533.5516185/94‑1 (MQ=60)
        AAACCAACGGCAGAAGCGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTA                                                                                       >  SRR4302533.1754995/1‑94 (MQ=60)
         AACCAACGGCAGAAGCGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTAT                                                                                      >  SRR4302533.4426328/1‑94 (MQ=60)
         AACCAACGGCAGAAGCGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTAT                                                                                      >  SRR4302533.6277888/1‑94 (MQ=60)
          ACCAACGGCAGAAGCGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATC                                                                                     <  SRR4302533.3824251/94‑1 (MQ=60)
          ACCAACGGCAGAAGCGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATC                                                                                     >  SRR4302533.4489361/1‑94 (MQ=60)
          ACCAACGGCAGAAGCGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATC                                                                                     >  SRR4302533.2160741/1‑94 (MQ=60)
          ACCAACGGCAGAAGCGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATC                                                                                     >  SRR4302533.5030296/1‑94 (MQ=60)
          ACCAACGGCAGAAGCGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATC                                                                                     <  SRR4302533.5015468/94‑1 (MQ=60)
           CCAACGGCAGAAGCGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCG                                                                                    >  SRR4302533.5972033/1‑94 (MQ=60)
           CCAACGGCAGAAGCGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCG                                                                                    <  SRR4302533.2936943/94‑1 (MQ=60)
           CCAACGGCAGAAGCGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCG                                                                                    >  SRR4302533.6288183/1‑94 (MQ=60)
            CAACGGCAGAAGCGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGC                                                                                   >  SRR4302533.750288/1‑94 (MQ=60)
            CAACGGCAGAAGCGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGC                                                                                   <  SRR4302533.1875496/94‑1 (MQ=60)
             AACGGCAGAAGCGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCG                                                                                  <  SRR4302533.1741284/94‑1 (MQ=60)
              ACGGCAGAAGCGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGC                                                                                 >  SRR4302533.6008342/1‑94 (MQ=60)
                 GCAGAAGCGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCA                                                                              <  SRR4302533.1692872/94‑1 (MQ=60)
                 GCAGAAGCGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCA                                                                              >  SRR4302533.4807070/1‑94 (MQ=60)
                  CAGAAGCGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAG                                                                             <  SRR4302533.5681115/94‑1 (MQ=60)
                   AGAAGCGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGA                                                                            >  SRR4302533.4693259/1‑94 (MQ=60)
                    GAGGCGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGAC                                                                           <  SRR4302533.5350805/94‑1 (MQ=60)
                    GAAGCGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGAC                                                                           <  SRR4302533.1751536/94‑1 (MQ=60)
                     AAGCGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACC                                                                          <  SRR4302533.5097908/94‑1 (MQ=60)
                      AGCGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCA                                                                         >  SRR4302533.4365812/1‑94 (MQ=60)
                      AGCGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGAGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCA                                                                         >  SRR4302533.5062350/1‑94 (MQ=60)
                         GCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGCAAGCAGGCGCTTCGCAAACACCGGTTATCGCGCGCAGACCAATT                                                                      >  SRR4302533.6054324/1‑94 (MQ=60)
                           GTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAA                                                                    >  SRR4302533.2647012/1‑94 (MQ=60)
                           GTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAA                                                                    <  SRR4302533.56657/94‑1 (MQ=60)
                           GTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAA                                                                    >  SRR4302533.446420/1‑94 (MQ=60)
                           GTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAA                                                                    >  SRR4302533.3307763/1‑94 (MQ=60)
                            TTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCGATTAAT                                                                   <  SRR4302533.3436032/94‑1 (MQ=60)
                            TTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAAT                                                                   <  SRR4302533.989120/94‑1 (MQ=60)
                             TCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATG                                                                  >  SRR4302533.4874473/1‑94 (MQ=60)
                             TCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATG                                                                  >  SRR4302533.850785/1‑94 (MQ=60)
                              CATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGA                                                                 <  SRR4302533.1766610/94‑1 (MQ=60)
                               ATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGAC                                                                <  SRR4302533.196274/94‑1 (MQ=60)
                               ATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGAC                                                                <  SRR4302533.2899045/94‑1 (MQ=60)
                               ATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACAACGGTTATCGCGCGCAGACCAATTAATGAC                                                                >  SRR4302533.1494386/1‑94 (MQ=60)
                                TGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACA                                                               <  SRR4302533.4997447/94‑1 (MQ=60)
                                 GTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACAT                                                              <  SRR4302533.3244148/94‑1 (MQ=60)
                                 GTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACAT                                                              >  SRR4302533.6004663/1‑94 (MQ=60)
                                 GTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACAT                                                              <  SRR4302533.5024155/94‑1 (MQ=60)
                                  TTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATT                                                             <  SRR4302533.2406991/94‑1 (MQ=60)
                                    CCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAA                                                           <  SRR4302533.4535209/94‑1 (MQ=60)
                                    CCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAA                                                           <  SRR4302533.2429046/94‑1 (MQ=60)
                                     CGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAG                                                          <  SRR4302533.3825243/94‑1 (MQ=60)
                                     CGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAG                                                          >  SRR4302533.3255405/1‑94 (MQ=60)
                                         AACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCG                                                      <  SRR4302533.22251/94‑1 (MQ=60)
                                         AACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCG                                                      <  SRR4302533.4920688/94‑1 (MQ=60)
                                         AACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCG                                                      >  SRR4302533.419006/1‑94 (MQ=60)
                                         AACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCG                                                      >  SRR4302533.204367/1‑94 (MQ=60)
                                          ACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGT                                                     <  SRR4302533.5374921/94‑1 (MQ=60)
                                           CCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTG                                                    <  SRR4302533.2865858/94‑1 (MQ=60)
                                           CCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTG                                                    <  SRR4302533.3873008/94‑1 (MQ=60)
                                             AGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGC                                                  <  SRR4302533.2751993/94‑1 (MQ=60)
                                             AGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGC                                                  <  SRR4302533.6137697/94‑1 (MQ=60)
                                              GCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCA                                                 <  SRR4302533.5814275/94‑1 (MQ=60)
                                                 GGTCACCCTCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAG                                              <  SRR4302533.3344926/94‑1 (MQ=60)
                                                 GGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAG                                              <  SRR4302533.4794494/94‑1 (MQ=60)
                                                 GGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAG                                              <  SRR4302533.5309646/94‑1 (MQ=60)
                                                 GGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAG                                              <  SRR4302533.4424555/94‑1 (MQ=60)
                                                  GTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGC                                             <  SRR4302533.2248318/94‑1 (MQ=60)
                                                   TCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACTAATTAATGACATTAAGCCCGTGGCAAAGCC                                            <  SRR4302533.2003789/94‑1 (MQ=60)
                                                   TCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCC                                            <  SRR4302533.5399077/94‑1 (MQ=60)
                                                    CACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCC                                           >  SRR4302533.578872/1‑94 (MQ=60)
                                                     ACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCT                                          >  SRR4302533.1388871/1‑94 (MQ=60)
                                                     ACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCT                                          <  SRR4302533.4961892/94‑1 (MQ=60)
                                                      CCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTG                                         <  SRR4302533.5075584/94‑1 (MQ=60)
                                                       CATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGG                                        <  SRR4302533.6194847/94‑1 (MQ=60)
                                                        ATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGA                                       >  SRR4302533.3868671/1‑94 (MQ=60)
                                                        ATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGA                                       <  SRR4302533.3056924/94‑1 (MQ=60)
                                                        ATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGA                                       >  SRR4302533.175167/1‑94 (MQ=60)
                                                        ATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGA                                       >  SRR4302533.2294165/1‑94 (MQ=60)
                                                         TCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAA                                      <  SRR4302533.5479984/94‑1 (MQ=60)
                                                         TCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAA                                      <  SRR4302533.3046968/94‑1 (MQ=60)
                                                           CGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATA                                    >  SRR4302533.6177139/1‑94 (MQ=60)
                                                            GGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAA                                   <  SRR4302533.2602494/94‑1 (MQ=60)
                                                            GGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAA                                   >  SRR4302533.2769730/1‑94 (MQ=60)
                                                            GGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAA                                   >  SRR4302533.1899617/1‑94 (MQ=60)
                                                              TTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAG                                 <  SRR4302533.870472/94‑1 (MQ=60)
                                                              TTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAG                                 >  SRR4302533.390189/1‑94 (MQ=60)
                                                               TATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGT                                <  SRR4302533.4999041/94‑1 (MQ=60)
                                                                ATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTC                               <  SRR4302533.348655/94‑1 (MQ=60)
                                                                 TTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCG                              <  SRR4302533.441564/94‑1 (MQ=60)
                                                                   GGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCC                            <  SRR4302533.2268485/94‑1 (MQ=60)
                                                                    GTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCA                           >  SRR4302533.1765765/1‑94 (MQ=60)
                                                                    GTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCA                           <  SRR4302533.6057559/94‑1 (MQ=60)
                                                                     TCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCAC                          <  SRR4302533.1855908/94‑1 (MQ=60)
                                                                      CGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACT                         >  SRR4302533.3056504/1‑94 (MQ=60)
                                                                      CGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACT                         <  SRR4302533.5690605/94‑1 (MQ=60)
                                                                      CGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACT                         >  SRR4302533.5722266/1‑94 (MQ=60)
                                                                       GGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACTG                        >  SRR4302533.5980349/1‑94 (MQ=60)
                                                                       GGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACTG                        >  SRR4302533.5980348/1‑94 (MQ=60)
                                                                       GGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACTG                        <  SRR4302533.3761697/94‑1 (MQ=60)
                                                                          AAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACTGACC                     <  SRR4302533.5814601/94‑1 (MQ=60)
                                                                          AAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACTGACC                     <  SRR4302533.1187051/94‑1 (MQ=60)
                                                                           AAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACTGACCA                    <  SRR4302533.2892383/94‑1 (MQ=60)
                                                                           AAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACTGACCA                    <  SRR4302533.4392814/94‑1 (MQ=60)
                                                                           AAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACTGACCA                    <  SRR4302533.4207977/94‑1 (MQ=60)
                                                                            AGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACTGACCAG                   <  SRR4302533.5250074/94‑1 (MQ=60)
                                                                            AGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACTGACCAG                   <  SRR4302533.4499090/94‑1 (MQ=60)
                                                                            AGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACTGACCAG                   <  SRR4302533.4658606/94‑1 (MQ=60)
                                                                             GCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACTGACCAGC                  >  SRR4302533.4750114/1‑94 (MQ=60)
                                                                              CAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACTGACCAGCC                 <  SRR4302533.2395042/94‑1 (MQ=60)
                                                                              CAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACTGACCAGCC                 <  SRR4302533.3254343/94‑1 (MQ=60)
                                                                               AGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACTGACCAGCCA                >  SRR4302533.1881461/1‑94 (MQ=60)
                                                                                  CGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACTGACCAGCCAAAT             >  SRR4302533.2476476/1‑94 (MQ=60)
                                                                                   GCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACTGACCAGCCAAATA            >  SRR4302533.267493/1‑94 (MQ=60)
                                                                                    CTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACTGACCAGCCAAATAT           >  SRR4302533.3816213/1‑94 (MQ=60)
                                                                                    CTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACTGACCAGCCAAATAT           <  SRR4302533.5392718/94‑1 (MQ=60)
                                                                                      TCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACTGACCAGCCAAATATCG         <  SRR4302533.451619/94‑1 (MQ=60)
                                                                                      TCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACTGACCAGCCAAATATCG         <  SRR4302533.4958290/94‑1 (MQ=60)
                                                                                        GAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACTGACCAGCCAAATATCGCA       >  SRR4302533.990755/1‑94 (MQ=60)
                                                                                         AAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACTGACCAGCCAAATATCGCAA      <  SRR4302533.3305698/94‑1 (MQ=60)
                                                                                          AAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACTGACCAGCCAAATATCGCAAT     <  SRR4302533.5013778/94‑1 (MQ=60)
                                                                                           AACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACTGACCAGCCAAATATCGCAATA    <  SRR4302533.316186/94‑1 (MQ=60)
                                                                                           AACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACTGACCAGCCAAATATCGCAATA    >  SRR4302533.5446168/1‑94 (MQ=60)
                                                                                             CACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACTGACCAGCCAAATATCGCAATAAA  >  SRR4302533.3495725/1‑94 (MQ=60)
                                                                                             CACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACTGACCAGCCAAATATCGCAATAAA  >  SRR4302533.4792368/1‑94 (MQ=60)
                                                                                             CACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACTGACCAGCCAAATATCGCAATAAA  >  SRR4302533.5019401/1‑94 (MQ=60)
                                                                                             |                                                                                             
ACGTATAAAAACCAACGGCAGAAGCGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAATACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACTGACCAGCCAAATATCGCAATAAA  >  NC_000913/3868919‑3869105

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑