Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,894,054	G→T	A62D (GCC→GAC)	yieG ←	putative membrane/transport protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,894,054	0	G	T	100.0%	883.4 / NA	242	A62D (GCC→GAC)	yieG	putative membrane/transport protein
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (132/110); total (132/110)

Only 100 of 260 total aligned reads displayed.

ATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGGCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTAAAACCGGCGATC  >  NC_000913/3893964‑3894143

aTAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGtcg                                                                                         >  1:4391109/1‑93 (MQ=255)

aTAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGtcg                                                                                         >  1:5451344/1‑93 (MQ=255)

aTAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGtcg                                                                                         >  2:3035356/1‑93 (MQ=255)

aTAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGtcg                                                                                         >  2:1630292/1‑93 (MQ=255)

  aGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCt                                                                                       <  2:3607796/93‑1 (MQ=255)

   gCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTg                                                                                      >  2:4983273/1‑93 (MQ=255)

   gCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTg                                                                                      <  2:2103841/93‑1 (MQ=255)

     gggTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGt                                                                                    >  1:5958222/1‑93 (MQ=255)

         gCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATcc                                                                                >  1:6236978/1‑93 (MQ=255)

          ccAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCa                                                                               <  1:92876/93‑1 (MQ=255)

              tGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGcc                                                                           >  2:4117833/1‑93 (MQ=255)

               gCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGGATCCATGCCa                                                                          >  2:1371359/1‑93 (MQ=255)

                cAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAg                                                                         <  2:4409356/93‑1 (MQ=255)

                 aaCTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGc                                                                        <  2:4995864/93‑1 (MQ=255)

                 aaCTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGc                                                                        <  1:2626285/93‑1 (MQ=255)

                 aaCTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGc                                                                        >  2:3884052/1‑93 (MQ=255)

                 aaCTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGc                                                                        <  2:3659121/93‑1 (MQ=255)

                    tGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAAc                                                                     <  2:710529/93‑1 (MQ=255)

                      gCAGGTTGGCAAACAGTCGCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGc                                                                   <  1:5752812/93‑1 (MQ=255)

                      gCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGc                                                                   <  2:4791507/93‑1 (MQ=255)

                       cAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGcc                                                                  <  2:5563000/93‑1 (MQ=255)

                        aGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCa                                                                 >  2:5866313/1‑93 (MQ=255)

                        aGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCa                                                                 >  2:4781636/1‑93 (MQ=255)

                        aGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCa                                                                 <  1:4214544/93‑1 (MQ=255)

                         ggTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCaa                                                                >  1:2232123/1‑93 (MQ=255)

                         ggTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCaa                                                                >  2:6303552/1‑93 (MQ=255)

                          gTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAg                                                               >  1:249694/1‑93 (MQ=255)

                          gTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAg                                                               <  1:2292888/93‑1 (MQ=255)

                          gTTGGCAAACAGTCACATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAg                                                               >  1:5859367/1‑93 (MQ=255)

                            tggCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGaa                                                             <  1:5331472/91‑1 (MQ=255)

                            tggCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGaa                                                             >  1:4428849/3‑93 (MQ=255)

                             ggCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAAt                                                            >  1:481656/2‑93 (MQ=255)

                                aaaCAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTg                                                         <  1:4492523/93‑1 (MQ=255)

                                aaaCAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTg                                                         >  2:2865490/1‑93 (MQ=255)

                                 aaCAGTCCCATCATAATACTGCCGAATGCAGCGATCAGGCAGGGAGTGACGAAGACGTCCCTGGTATCCATGCCAGCAACGCCAAGAATTTGc                                                        <  2:5667717/93‑1 (MQ=255)

                                 aaCAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGc                                                        <  2:1113348/93‑1 (MQ=255)

                                 aaCAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGc                                                        >  1:745800/1‑93 (MQ=255)

                                 aaCAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGc                                                        <  2:2849630/93‑1 (MQ=255)

                                     gTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGt                                                    <  1:4147681/93‑1 (MQ=255)

                                       cccATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTa                                                  >  1:3735612/1‑93 (MQ=255)

                                        ccATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTaa                                                 >  2:1199051/1‑93 (MQ=255)

                                        ccATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTaa                                                 <  2:4594461/93‑1 (MQ=255)

                                         catcatAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAAc                                                >  2:3914956/1‑93 (MQ=255)

                                         catcatAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAAc                                                <  1:6192107/93‑1 (MQ=255)

                                           tcatAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACaa                                              >  2:4135136/1‑93 (MQ=255)

                                            catAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACaaa                                             >  1:1370887/1‑93 (MQ=255)

                                             ataataCTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACaaaa                                            >  1:6081792/1‑93 (MQ=255)

                                              taataCTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACaaaaa                                           >  2:5789112/1‑93 (MQ=255)

                                               aataCTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAAc                                          <  1:3792465/93‑1 (MQ=255)

                                                 taCTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGa                                        <  2:5431491/93‑1 (MQ=255)

                                                 taCTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGa                                        <  1:4039736/93‑1 (MQ=255)

                                                 taCTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGa                                        <  1:1481726/93‑1 (MQ=255)

                                                  aCTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCTAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGAt                                       >  1:3996468/1‑93 (MQ=255)

                                                  aCTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGAt                                       <  2:4030041/93‑1 (MQ=255)

                                                   cTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATg                                      >  1:2453600/1‑93 (MQ=255)

                                                    tGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCTAAGAATTTGCGGGTTAACAAAAACGATGt                                     <  1:2443811/93‑1 (MQ=255)

                                                    tGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGt                                     >  2:2665762/1‑93 (MQ=255)

                                                    tGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACACCAAGAATTTGCGGGTTAACAAAAACGATGt                                     >  1:6003941/1‑93 (MQ=255)

                                                     gCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTa                                    >  1:5523104/1‑93 (MQ=255)

                                                     gCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTa                                    <  2:5060386/93‑1 (MQ=255)

                                                      ccGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTaa                                   >  2:3720126/1‑93 (MQ=255)

                                                      ccGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTaa                                   <  2:6240175/93‑1 (MQ=255)

                                                       cGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTaaa                                  >  2:1646062/1‑93 (MQ=255)

                                                       cGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTaaa                                  <  1:3506919/93‑1 (MQ=255)

                                                        gAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAAc                                 <  2:3774135/93‑1 (MQ=255)

                                                        gAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAAc                                 >  1:33072/1‑93 (MQ=255)

                                                        gAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAAc                                 <  1:5198083/93‑1 (MQ=255)

                                                        gAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGCGCTAACAAAAACGATGTAAAc                                 >  1:1570345/1‑93 (MQ=255)

                                                          aTGGAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCa                               <  2:2500246/93‑1 (MQ=255)

                                                          aTGCGGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCa                               <  2:1905167/93‑1 (MQ=255)

                                                          aTGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCa                               >  1:1236015/1‑93 (MQ=255)

                                                          aTGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCa                               >  2:2334499/1‑93 (MQ=255)

                                                            gCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATc                             >  1:2798360/1‑93 (MQ=255)

                                                            gCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATc                             <  2:5516256/93‑1 (MQ=255)

                                                            gCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATc                             <  2:5334441/93‑1 (MQ=255)

                                                            gCAGCGATAAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATc                             <  2:4320612/93‑1 (MQ=255)

                                                             cAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCg                            >  2:5398094/1‑93 (MQ=255)

                                                              aGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGt                           >  2:3359286/1‑93 (MQ=255)

                                                              aGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGt                           <  1:5038760/93‑1 (MQ=255)

                                                               gCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTc                          >  2:5322033/1‑93 (MQ=255)

                                                               gCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTc                          >  1:629543/1‑93 (MQ=255)

                                                                cGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCa                         <  1:3042016/93‑1 (MQ=255)

                                                                 gATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAg                        <  2:6065298/93‑1 (MQ=255)

                                                                  aTCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAgg                       >  1:4999057/1‑93 (MQ=255)

                                                                  aTCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAgg                       >  1:3197369/1‑93 (MQ=255)

                                                                   tCAGACAGGGAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGa                      >  2:623094/1‑93 (MQ=255)

                                                                     aGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAg                    <  2:1254614/93‑1 (MQ=255)

                                                                      gACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAgg                   >  2:1956803/1‑93 (MQ=255)

                                                                        cAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAggtg                 >  1:1961961/1‑93 (MQ=255)

                                                                         aGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAggtgg                <  1:5646796/93‑1 (MQ=255)

                                                                          ggTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAggtgga               >  1:4134371/1‑92 (MQ=255)

                                                                           gTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAggtggga              >  1:4863840/1‑91 (MQ=255)

                                                                            tAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTaa             >  2:931103/1‑93 (MQ=255)

                                                                               tGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTAAAAc          <  1:1146812/93‑1 (MQ=255)

                                                                               tGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGCTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTAAAAc          >  2:383565/1‑93 (MQ=255)

                                                                                 aCGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTAAAACCg        >  1:3400149/1‑93 (MQ=255)

                                                                                 aCGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTAAAACCg        >  1:656224/1‑93 (MQ=255)

                                                                                 aCGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTAAAACCg        >  1:6163613/1‑93 (MQ=255)

                                                                                     aGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTAAAACCGGCGa    >  1:1702649/1‑93 (MQ=255)

                                                                                       aCGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTAAAACCGGCGATc  >  1:2966433/1‑93 (MQ=255)

ATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGGCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTAAAACCGGCGATC  >  NC_000913/3893964‑3894143

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGGCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTAAAACCGGCGATCACTT  >  NC_000913/3893962‑3894147

CCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTC                                                                                              >  SRR4302533.750536/1‑94 (MQ=60)

 CATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCG                                                                                             >  SRR4302533.4417617/1‑94 (MQ=60)

 CATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCG                                                                                             >  SRR4302533.2462929/1‑94 (MQ=60)

 CATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCG                                                                                             >  SRR4302533.326567/1‑94 (MQ=60)

 CATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCG                                                                                             >  SRR4302533.1620968/1‑94 (MQ=60)

 CATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCG                                                                                             >  SRR4302533.5484183/1‑94 (MQ=60)

  ATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGC                                                                                            >  SRR4302533.5004821/1‑94 (MQ=60)

  ATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGC                                                                                            >  SRR4302533.3086865/1‑94 (MQ=60)

    AGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTG                                                                                          >  SRR4302533.6165749/1‑94 (MQ=60)

      CGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGT                                                                                        <  SRR4302533.4166446/94‑1 (MQ=60)

      CGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGT                                                                                        >  SRR4302533.5994261/1‑94 (MQ=60)

          TGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCC                                                                                    >  SRR4302533.2501488/1‑94 (MQ=60)

          TGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCC                                                                                    >  SRR4302533.3240903/1‑94 (MQ=60)

          TGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCC                                                                                    <  SRR4302533.2641169/94‑1 (MQ=60)

          TGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCC                                                                                    >  SRR4302533.6274325/1‑94 (MQ=60)

           GCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCA                                                                                   <  SRR4302533.5275009/94‑1 (MQ=60)

           GCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCA                                                                                   <  SRR4302533.710294/94‑1 (MQ=60)

           GCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCA                                                                                   >  SRR4302533.3711272/1‑94 (MQ=60)

            CCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCAT                                                                                  <  SRR4302533.93464/94‑1 (MQ=60)

             CAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATG                                                                                 <  SRR4302533.3971799/94‑1 (MQ=60)

               GTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCC                                                                               <  SRR4302533.5996646/94‑1 (MQ=60)

                   AACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCA                                                                           <  SRR4302533.2642398/94‑1 (MQ=60)

                     CTGGCAGGTTGGCAAACAGTCCCATCATCATGCTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAAC                                                                         <  SRR4302533.2700046/94‑1 (MQ=60)

                     CTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAAC                                                                         >  SRR4302533.2181063/1‑94 (MQ=60)

                      TGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATatacagcaga                                                                        <  SRR4302533.420543/94‑11 (MQ=60)

                      TGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACG                                                                        <  SRR4302533.3958395/94‑1 (MQ=60)

                      TGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACG                                                                        <  SRR4302533.4481432/94‑1 (MQ=60)

                      TGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACG                                                                        >  SRR4302533.4200620/1‑94 (MQ=60)

                      TGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGGAGTGACGAAGACGTCGCTGGGATCCACGCCAGCAACG                                                                        >  SRR4302533.846493/1‑94 (MQ=60)

                       GGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGC                                                                       >  SRR4302533.3741974/1‑94 (MQ=60)

                        GCAGGTTGGCAAACAGTCGCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCC                                                                      <  SRR4302533.5787521/94‑1 (MQ=60)

                        GCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCC                                                                      <  SRR4302533.198285/94‑1 (MQ=60)

                        GCAAGTTGGCAAACAGTCCCATCATAATCCTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCC                                                                      <  SRR4302533.4240049/94‑1 (MQ=60)

                          AGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAA                                                                    <  SRR4302533.4240041/94‑1 (MQ=60)

                          AGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAA                                                                    >  SRR4302533.2245894/1‑94 (MQ=60)

                           GGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAG                                                                   <  SRR4302533.643754/94‑1 (MQ=60)

                           GGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAG                                                                   >  SRR4302533.2545257/1‑94 (MQ=60)

                           GGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAG                                                                   >  SRR4302533.251235/1‑94 (MQ=60)

                           GGTTGGCAAACAGTCACATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAG                                                                   >  SRR4302533.5894790/1‑94 (MQ=60)

                            GTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGA                                                                  <  SRR4302533.2307083/94‑1 (MQ=60)

                             TTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAA                                                                 >  SRR4302533.4455623/1‑94 (MQ=60)

                              TGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAAT                                                                <  SRR4302533.5363428/94‑1 (MQ=60)

                              TGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAAT                                                                >  SRR4302533.5084416/1‑94 (MQ=60)

                              TGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAAT                                                                >  SRR4302533.675948/1‑94 (MQ=60)

                              TGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAAT                                                                >  SRR4302533.484533/1‑94 (MQ=60)

                                 CAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTG                                                             <  SRR4302533.6206715/94‑1 (MQ=60)

                                  AAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGC                                                            >  SRR4302533.4119325/1‑94 (MQ=60)

                                  AAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGC                                                            >  SRR4302533.750189/1‑94 (MQ=60)

                                  AAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGC                                                            <  SRR4302533.4519729/94‑1 (MQ=60)

                                   AACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCG                                                           >  SRR4302533.3431958/1‑94 (MQ=60)

                                   AACACACCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACCTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCG                                                           <  SRR4302533.1406729/94‑1 (MQ=60)

                                    ACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGG                                                          <  SRR4302533.2866751/94‑1 (MQ=60)

                                    ACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGG                                                          >  SRR4302533.5443441/1‑94 (MQ=60)

                                     CAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGG                                                         <  SRR4302533.6239584/94‑1 (MQ=60)

                                       GTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTT                                                       <  SRR4302533.4172810/94‑1 (MQ=60)

                                        TCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTA                                                      >  SRR4302533.3758530/1‑94 (MQ=60)

                                        TCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTA                                                      >  SRR4302533.1686083/1‑94 (MQ=60)

                                         CCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAA                                                     <  SRR4302533.5872157/94‑1 (MQ=60)

                                           CATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACA                                                   <  SRR4302533.6229267/94‑1 (MQ=60)

                                           CATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACA                                                   >  SRR4302533.1943907/1‑94 (MQ=60)

                                            ATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAA                                                  <  SRR4302533.4637588/94‑1 (MQ=60)

                                             TCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAA                                                 >  SRR4302533.1379126/1‑94 (MQ=60)

                                              CATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAA                                                <  SRR4302533.4169738/94‑1 (MQ=60)

                                              CATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAA                                                >  SRR4302533.6118569/1‑94 (MQ=60)

                                              CATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAA                                                >  SRR4302533.1572885/1‑94 (MQ=60)

                                              CATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATACATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAA                                                >  SRR4302533.1539376/1‑94 (MQ=60)

                                              CATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGGATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAA                                                >  SRR4302533.2460520/1‑94 (MQ=60)

                                                 AATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACG                                             <  SRR4302533.1235761/94‑1 (MQ=60)

                                                 AATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACG                                             >  SRR4302533.411549/1‑94 (MQ=60)

                                                 AATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACG                                             <  SRR4302533.3815680/94‑1 (MQ=60)

                                                  ATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGA                                            >  SRR4302533.2267704/1‑94 (MQ=60)

                                                  ATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGA                                            <  SRR4302533.5172656/94‑1 (MQ=60)

                                                   TACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCTAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGAT                                           >  SRR4302533.4020805/1‑94 (MQ=60)

                                                   TACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGAT                                           <  SRR4302533.4064321/94‑1 (MQ=60)

                                                   TACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGAT                                           <  SRR4302533.6226334/94‑1 (MQ=60)

                                                   TACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGAT                                           <  SRR4302533.1490671/94‑1 (MQ=60)

                                                    ACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATG                                          >  SRR4302533.2468740/1‑94 (MQ=60)

                                                     CTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACACCAAGAATTTGCGGGTTAACAAAAACGATGT                                         >  SRR4302533.6040318/1‑94 (MQ=60)

                                                      TGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCTAAGAATTTGCGGGTTAACAAAAACGATGTA                                        <  SRR4302533.2458895/94‑1 (MQ=60)

                                                      TGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTA                                        >  SRR4302533.5556450/1‑94 (MQ=60)

                                                      TGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTA                                        <  SRR4302533.6313288/94‑1 (MQ=60)

                                                        CCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAA                                      >  SRR4302533.4947242/1‑94 (MQ=60)

                                                         CGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAAC                                     <  SRR4302533.4641134/94‑1 (MQ=60)

                                                         CGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAAC                                     >  SRR4302533.2948659/1‑94 (MQ=60)

                                                         CGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAAC                                     <  SRR4302533.2428415/94‑1 (MQ=60)

                                                         CGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAAC                                     <  SRR4302533.3528544/94‑1 (MQ=60)

                                                         CGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAAC                                     >  SRR4302533.33295/1‑94 (MQ=60)

                                                         CGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGCGCTAACAAAAACGATGTAAAC                                     >  SRR4302533.1579803/1‑94 (MQ=60)

                                                         CGAATGCAGCGATCAGACAGGGAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAAC                                     >  SRR4302533.5300540/1‑94 (MQ=60)

                                                          GAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACC                                    >  SRR4302533.5717837/1‑94 (MQ=60)

                                                          GAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACC                                    <  SRR4302533.5229430/94‑1 (MQ=60)

                                                           AATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCA                                   >  SRR4302533.6107264/1‑94 (MQ=60)

                                                           AATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCA                                   <  SRR4302533.3380288/94‑1 (MQ=60)

                                                           AATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCA                                   >  SRR4302533.1243403/1‑94 (MQ=60)

                                                             TGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATC                                 >  SRR4302533.2815573/1‑94 (MQ=60)

                                                             TGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATC                                 <  SRR4302533.4507066/94‑1 (MQ=60)

                                                             TGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATC                                 <  SRR4302533.1319628/94‑1 (MQ=60)

                                                              GCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCG                                <  SRR4302533.579889/94‑1 (MQ=60)

                                                              GCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCG                                <  SRR4302533.5636532/94‑1 (MQ=60)

                                                              GCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCG                                <  SRR4302533.550698/94‑1 (MQ=60)

                                                               CAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGT                               <  SRR4302533.6314279/94‑1 (MQ=60)

                                                               CAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATAGT                               >  SRR4302533.6004571/1‑94 (MQ=60)

                                                                AGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTC                              <  SRR4302533.5069268/94‑1 (MQ=60)

                                                                AGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTC                              >  SRR4302533.633244/1‑94 (MQ=60)

                                                                  CGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAG                            <  SRR4302533.3060637/94‑1 (MQ=60)

                                                                  CGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAG                            <  SRR4302533.1475177/94‑1 (MQ=60)

                                                                   GATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGG                           <  SRR4302533.3021509/94‑1 (MQ=60)

                                                                   GATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGG                           >  SRR4302533.3217047/1‑94 (MQ=60)

                                                                   GATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGG                           >  SRR4302533.5029361/1‑94 (MQ=60)

                                                                       AGACAGGTAGTGACGAAGACGTCGCTGGTATCCGTGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGG                       >  SRR4302533.5753579/1‑94 (MQ=60)

                                                                         ACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTG                     >  SRR4302533.1973937/1‑94 (MQ=60)

                                                                         ACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTG                     <  SRR4302533.1287335/94‑1 (MQ=60)

                                                                         ACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTG                     <  SRR4302533.912849/94‑1 (MQ=60)

                                                                          CAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGG                    >  SRR4302533.4263815/1‑94 (MQ=60)

                                                                           AGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGT                   <  SRR4302533.5680933/94‑1 (MQ=60)

                                                                           AGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGA                   >  SRR4302533.4159465/1‑94 (MQ=60)

                                                                            GGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTA                  >  SRR4302533.3779954/1‑94 (MQ=60)

                                                                            GGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGGA                  >  SRR4302533.4893314/1‑94 (MQ=60)

                                                                             GTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTAA                 >  SRR4302533.5962983/1‑94 (MQ=60)

                                                                               AGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTAAAA               >  SRR4302533.4443207/1‑94 (MQ=60)

                                                                                GTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTAAATC              <  SRR4302533.5527252/94‑1 (MQ=60)

                                                                                GTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTAAAAC              <  SRR4302533.2346413/94‑1 (MQ=60)

                                                                                 TGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTAAAACC             <  SRR4302533.1153657/94‑1 (MQ=60)

                                                                                 TGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGCTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTAAAACC             >  SRR4302533.4876848/1‑94 (MQ=60)

                                                                                 TAACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTAAAACC             <  SRR4302533.3474877/94‑1 (MQ=60)

                                                                                  GACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTAAAACCG            >  SRR4302533.6200669/1‑94 (MQ=60)

                                                                                  GACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTAAAACCG            >  SRR4302533.3421144/1‑94 (MQ=60)

                                                                                  GACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTAAAACCG            >  SRR4302533.660096/1‑94 (MQ=60)

                                                                                    CGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTAAAACCGGC          <  SRR4302533.5040474/94‑1 (MQ=60)

                                                                                    CGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTAAAACCGGC          >  SRR4302533.4055343/1‑94 (MQ=60)

                                                                                    CGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTAAAACCGGC          <  SRR4302533.3053932/94‑1 (MQ=60)

                                                                                    CGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTAAAACCGGC          >  SRR4302533.6198028/1‑94 (MQ=60)

                                                                                      AAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTAAAACCGGCGA        >  SRR4302533.1712965/1‑94 (MQ=60)

                                                                                        GACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTAAAACCGGCGATC      >  SRR4302533.2984564/1‑94 (MQ=60)

                                                                                         ACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTAAAACCGGCGATCA     >  SRR4302533.980430/1‑94 (MQ=60)

                                                                                          CGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGATGGTGGTAAAACCGGCGATCAC    <  SRR4302533.2088027/94‑1 (MQ=60)

                                                                                           GTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTAAAACCGGCGATCACT   >  SRR4302533.5023626/1‑94 (MQ=60)

                                                                                           GTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTAAAACCGGCGATCACT   >  SRR4302533.4170160/1‑94 (MQ=60)

                                                                                           GTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTAAAACCGGCGATCACT   >  SRR4302533.5467031/1‑94 (MQ=60)

                                                                                            TCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTAAAACCGGCGATCACTT  <  SRR4302533.4978251/94‑1 (MQ=60)

CCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGGCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTAAAACCGGCGATCACTT  >  NC_000913/3893962‑3894147

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`