Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,899,957 (C)5→6 coding (1373/1413 nt) bglB ← phospho‑beta‑glucosidase B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,899,9521.C99.1% 704.6 / ‑3.9 225L460V (CTG→GTG) bglBphospho‑beta‑glucosidase B
Reads supporting (aligned to +/- strand):  ref base . (1/1);  major base C (90/131);  minor base A (2/0);  total (93/132)
Fisher's exact test for biased strand distribution p-value = 1.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

Only 100 of 266 total aligned reads displayed.

ATGGTGCAAAGAAAGAATTAACGCATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGAC‑AG‑CCCCCGCGTCTTGATCACCTCTGCATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGAT  >  NC_000913/3899860‑3900038
                                                                                              |                                                                                      
 tGGTGCAAAGAAAGAATTAACGCATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGAC‑AG‑c                                                                                       >  1:702538/1‑93 (MQ=255)
 tGGTGCAAAGAAAGAATTAACGCATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGAC‑AG‑c                                                                                       <  2:2008426/93‑1 (MQ=255)
 tGGTGCAAAGAAAGAATTAACGCATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGAC‑AG‑c                                                                                       <  2:39794/93‑1 (MQ=255)
  ggTGCAAAGAAAGAATTAACGCATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGAC‑AG‑cc                                                                                      >  1:1009383/1‑93 (MQ=255)
    tGCAAAGAAAGAATTAACGCATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGAC‑AG‑cccc                                                                                    <  1:2754232/93‑1 (MQ=255)
     gCAAAGAAAGAATTAACGCATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGAC‑AG‑ccccc                                                                                   >  1:4876683/1‑93 (MQ=255)
     gCAAAGAAAGAATTAACGCATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGAC‑AG‑ccccc                                                                                   >  1:5716798/1‑93 (MQ=255)
     gCAAAGAAAGAATTAACGCATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGAC‑AG‑ccccc                                                                                   <  2:3744090/93‑1 (MQ=255)
      cAAAGAAAGAATTAACGCATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGAC‑AG‑cccccc                                                                                  <  1:5276347/93‑2 (MQ=255)
      cAAAGAAAGAATTAACGCATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGAC‑AG‑cccccc                                                                                  <  2:4534136/93‑2 (MQ=255)
      cAAAGAAAGAATTAACGCATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGAC‑AG‑cccccc                                                                                  >  2:643363/1‑92 (MQ=255)
       aaagaaagAATTAACGCATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGAC‑AG‑ccccccg                                                                                 >  2:2039028/1‑91 (MQ=255)
       aaagaaagAATTAACGCATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGAC‑AG‑ccccccg                                                                                 >  1:2935355/1‑91 (MQ=255)
        aagaaagaATTAACGCATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGAC‑AG‑ccccccgc                                                                                >  2:257152/1‑90 (MQ=255)
        aagaaagaATTAACGCATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGAC‑AG‑ccccccgc                                                                                >  1:2905265/1‑90 (MQ=255)
           aaagaaTTAACGCATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGAC‑AGCCCCCCGCGTc                                                                              >  1:5087380/1‑93 (MQ=255)
           aaagaaTTAACGCATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGAC‑AGCCCCCCGCGTc                                                                              >  2:2895343/1‑93 (MQ=255)
           aaagaaTTAACGCATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGAC‑AGCCCCCCGCGTc                                                                              <  1:2497651/93‑1 (MQ=255)
            aagaaTTAACGCATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGAC‑AGCCCCCCGCGTCt                                                                             >  1:315988/1‑93 (MQ=255)
            aagaaTTAACGCATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGAC‑AGCCCCCCGCGTCt                                                                             <  2:753242/93‑1 (MQ=255)
            aagaaTTAACGCATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGAC‑AGCCCCCCGCGTCt                                                                             <  1:5939046/93‑1 (MQ=255)
            aagaaTTAACGCATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGAC‑AGCCCCCCGCGTCt                                                                             <  2:5542836/93‑1 (MQ=255)
             agaaTTAACGCATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGAC‑AGCCCCCCGCGTCtt                                                                            <  1:6041041/93‑1 (MQ=255)
             agaaTTAACGCATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGAC‑AGCCCCCCGCGTCtt                                                                            >  1:2007072/1‑93 (MQ=255)
              gaaTTAACGCATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGAC‑AGCCCCCCGCGTCTTg                                                                           <  2:2280303/93‑1 (MQ=255)
              gaaTTAACGCATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGAC‑AGCCCCCCGCGTCTTg                                                                           <  2:2666297/93‑1 (MQ=255)
                    aCGCATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGAC‑AGCCCCCCGCGTCTTTATTAcc                                                                     >  2:3271226/1‑93 (MQ=255)
                    aCGCATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGAC‑AGCCCCCCGCGTCTTGATCAcc                                                                     >  1:3871444/1‑93 (MQ=255)
                       catcgcatcCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGAC‑AGCCCCCCGCGTCTTGATCACctct                                                                  <  1:2667879/93‑1 (MQ=255)
                        atcgcatcCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGAC‑AGACCCCCGCGTCTTGATCACctcta                                                                 >  2:5109881/1‑92 (MQ=255)
                         tcgcatcCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGAC‑AGCCCCCCGCGTCTTGATCACctctac                                                                >  1:910112/1‑91 (MQ=255)
                         tcgcatcCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGAC‑AGCCCCCCGCGTCTTGATCACctctac                                                                >  2:1783434/1‑91 (MQ=255)
                          cgcatcCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGAC‑AGCCCCCCGCGTCTTGATCACCTCTACa                                                               >  1:2237133/1‑93 (MQ=255)
                           gcatcCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGAC‑AGCCCCCCGCGTCTTGATCACCTCTACAt                                                              <  2:5988275/93‑1 (MQ=255)
                            catcCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGAC‑AGCCCCCCGCGTCTTGATCACCTCTACATa                                                             >  1:144052/1‑93 (MQ=255)
                             atcCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGAC‑AGCCCCCCGCGTCTTGATCACCTCTACATAc                                                            >  2:2743827/1‑93 (MQ=255)
                              tcCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGAC‑AGCCCCCCGCGTCTTGATCACCTCTACATAcc                                                           <  2:2366406/93‑1 (MQ=255)
                                 aGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGAC‑AGCCCCCCGCGTCTTGATCACCTCTACATACCATc                                                        <  2:2471539/93‑1 (MQ=255)
                                 aGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGAC‑AGCCCCCCGCGTCTTGATCACCTCTACATACCATc                                                        <  2:2914383/93‑1 (MQ=255)
                                 aGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGAC‑AGCCCCCCGCGTCTTGATCACCTCTACATACCATc                                                        <  2:322201/93‑1 (MQ=255)
                                  gACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGAC‑AGCCCCCCGCGTCTTGATCACCTCTACATACCATcc                                                       <  1:4271651/93‑1 (MQ=255)
                                    cTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGAC‑AGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGa                                                     <  1:4507710/93‑1 (MQ=255)
                                    cTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGAC‑AGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGa                                                     >  1:1853161/1‑93 (MQ=255)
                                     tGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGAC‑AGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGaa                                                    <  1:5470047/93‑1 (MQ=255)
                                      gTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGAC‑AGCCCCCCGCGTCTTGATCATCTCTACATACCATCCGAAg                                                   <  2:6083469/93‑1 (MQ=255)
                                        tCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGAC‑AGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCt                                                 <  1:1032921/93‑1 (MQ=255)
                                         cTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGAC‑AGCCCCCCGCGTCTTGATCCCCTCTACATGCCATCCGAAGCtt                                                <  1:3458301/93‑1 (MQ=255)
                                         cTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGAC‑AGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCtt                                                <  2:1262077/93‑1 (MQ=255)
                                         cTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGAC‑AGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCtt                                                >  1:1885149/1‑93 (MQ=255)
                                          tGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGAC‑AGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCttt                                               <  2:4527366/93‑1 (MQ=255)
                                          tGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGAC‑AGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCttt                                               <  1:5168283/93‑1 (MQ=255)
                                            aaTGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGAC‑AGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTc                                             >  2:1491303/1‑93 (MQ=255)
                                             aTGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACGTC‑CCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCt                                            <  2:500107/93‑1 (MQ=255)
                                             aTGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGAC‑AGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCt                                            <  1:6259550/93‑1 (MQ=255)
                                             aTGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGAC‑AGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCt                                            <  1:1420408/93‑1 (MQ=255)
                                              tGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGAC‑AGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCtt                                           <  2:3934260/93‑1 (MQ=255)
                                               gCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGAC‑AGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTg                                          <  2:4487088/93‑1 (MQ=255)
                                               gCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGAC‑AGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTg                                          <  2:1005047/93‑1 (MQ=255)
                                                cgacgaTAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGAC‑AGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGc                                         <  2:3672332/93‑1 (MQ=255)
                                                    gaTAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGAC‑AGCCCCCCGCGGCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTCCgtgt                                     >  1:4096154/1‑93 (MQ=255)
                                                     aTAATTAAGGGGGTTTTATGGGTATTTTTTTTAATGAC‑AGCCCCCCGCGTCCTGATCACCTCTTCATTACATTCGAAGCTTTTCTTGTGtttt                                    >  2:4834182/1‑93 (MQ=255)
                                                      tAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGAC‑AGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTc                                   <  2:5864785/93‑1 (MQ=255)
                                                       aaTTAAGGTGCTTTAATGGTTATTTTTTTTAATGAC‑AGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCt                                  >  2:5652613/1‑93 (MQ=255)
                                                       aaTTAAGGTGCTTTAATGGTTATTTTTTTTAATGAC‑AGACCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCt                                  >  2:582176/1‑93 (MQ=255)
                                                        aTTAAGGTGCTTTAATGGTTATTTTTTTTAATGAC‑AGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCtt                                 <  1:4748764/93‑1 (MQ=255)
                                                        aTTAAGGTGCTTTAATGGTTATTTTTTTTAATGAC‑AGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCtt                                 <  2:1333444/93‑1 (MQ=255)
                                                         ttAAGGTGCTTTAATGGTTATTTTTTTTAATGAC‑AGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTtg                                <  2:4182892/93‑1 (MQ=255)
                                                          tAAGGTTGTTTAATGGTTTTTTTTTTTAATGAC‑AGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTtgt                               <  2:5602877/93‑1 (MQ=255)
                                                            aGGTGCTTTAATGGTTATTTTTTTTAATGAC‑AGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGa                             <  2:308588/93‑1 (MQ=255)
                                                            aGGTGCTTTAATGGTTATTTTTTTTAATGAC‑AGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGa                             <  1:1166974/93‑1 (MQ=255)
                                                            aGGTGCTTTAATGGTTATTTTTTTTAATGAC‑AGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGa                             <  1:5405247/93‑1 (MQ=255)
                                                            aGGTGCTTTAATGGTTATTTTTTTTAATGAC‑AGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGa                             <  2:4505037/93‑1 (MQ=255)
                                                             agggCTTTTAAGGGTATTTTTTTTAATGAC‑AGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAg                            <  2:1907876/90‑1 (MQ=255)
                                                                gCTTTAATGGTTATTTTTTTTAATGAC‑AGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGct                         <  2:4731745/93‑1 (MQ=255)
                                                                gCTTTAATGGTTATTTTTTTTAATGAC‑AGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGct                         >  2:436605/1‑93 (MQ=255)
                                                                gCTTTAATGGTTATTTTTTTTAATGAC‑AGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGct                         <  1:1326693/93‑1 (MQ=255)
                                                                 cTTTAATGGTTATTTTTTTTAATGAC‑AGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGctt                        >  2:1975461/1‑93 (MQ=255)
                                                                  tttAATGTTTATTTTTTTTAATGAC‑AGCCCCCCGCGTCTTGATCCCCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGcttc                       <  2:1649686/93‑1 (MQ=255)
                                                                  tttAATGGTTATTTTTTTTAATGAC‑AGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGcttc                       >  1:6176899/1‑93 (MQ=255)
                                                                  tttAATGGTTATTTTTTTTAATGAC‑AGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGcttc                       >  1:3294696/1‑93 (MQ=255)
                                                                   ttAATGGTTATTTTTTTTAATGAC‑AGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGcttcc                      <  2:1824844/93‑1 (MQ=255)
                                                                     aaTGGTTATTTTTTTTAATGAC‑AGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGcttcctt                    >  1:3040339/1‑93 (MQ=255)
                                                                     aaTGGTTATTTTTTTTAATGAC‑AGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGcttcctt                    >  1:5625482/1‑93 (MQ=255)
                                                                     aaTGGTTATTTTTTTTAATGAC‑AGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGcttcctt                    <  2:2201849/93‑1 (MQ=255)
                                                                     aaTGGTTATTTTTTTTAATGAC‑AGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGcttcctt                    <  1:2567652/93‑1 (MQ=255)
                                                                     aaTGGTTATTTTTTTTAATGAC‑AGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGcttcctt                    <  1:4645994/93‑1 (MQ=255)
                                                                      aTGGTTATTTTTTTTAATGAC‑AGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGcttccttc                   >  1:113484/1‑93 (MQ=255)
                                                                       tGGTTATTTTTTTTAATGAC‑AGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGcttccttcc                  >  1:5183004/1‑92 (MQ=255)
                                                                           tATTTTTTTTAATGAC‑AGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCAt              <  2:4896629/93‑1 (MQ=255)
                                                                            aTTTTTTTTAATGAC‑AGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCAtt             <  1:4181213/93‑1 (MQ=255)
                                                                             ttttttttAATGAC‑AGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCCTTa            >  2:5194043/1‑93 (MQ=255)
                                                                             ttttttttAATGAC‑AGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTa            <  1:2156055/93‑1 (MQ=255)
                                                                                tttttAATGAC‑AGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTatca         >  2:5357175/1‑93 (MQ=255)
                                                                                 ttttAATGAC‑AGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTatcat        <  1:5209320/93‑1 (MQ=255)
                                                                                  tttAATGAC‑AGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTatcatc       >  1:1554960/1‑93 (MQ=255)
                                                                                    tAATGAC‑AGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCAc     >  1:3825990/1‑93 (MQ=255)
                                                                                    tAATGAC‑AGCCCCCCGCGGCTTGAGCACCTCTACATACCATCCGAAGGTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCCATATCAGCAc     >  2:3526380/1‑93 (MQ=255)
                                                                                     aaTGAC‑AGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACg    >  1:3957303/1‑93 (MQ=255)
                                                                                     aaTGAC‑AGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACg    >  2:3788296/1‑93 (MQ=255)
                                                                                      aTGAC‑AGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGa   >  1:5808922/1‑93 (MQ=255)
                                                                                              |                                                                                      
ATGGTGCAAAGAAAGAATTAACGCATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGAC‑AG‑CCCCCGCGTCTTGATCACCTCTGCATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGAT  >  NC_000913/3899860‑3900038

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGCAAAGAAAGAATTAACGCATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAG‑CCCCCGCGTCTTGATCACCTCTGCATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAA  >  NC_000913/3899864‑3900050
                                                                                              |                                                                                             
TGCAAAGAAAGAATTAACGCATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAG‑CCCCC                                                                                               >  SRR4302533.3950019/1‑94 (MQ=60)
TGCAAAGAAAGAATTAACGCATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAG‑CCCCC                                                                                               >  SRR4302533.4070688/1‑94 (MQ=60)
TGCAAAGAAAGAATTAACGCATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAG‑CCCCC                                                                                               <  SRR4302533.2771123/94‑1 (MQ=60)
TGCAAAGAAAGAATTAACGCATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAG‑CCCCC                                                                                               >  SRR4302533.4906228/1‑94 (MQ=60)
TGCAAAGAAAGAATTAACGCATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAG‑CCCCC                                                                                               <  SRR4302533.1981830/94‑1 (MQ=60)
TGCAAAGAAAGAATTAACGCATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAG‑CCCCC                                                                                               >  SRR4302533.5751324/1‑94 (MQ=60)
  CAAAGAAAGAATTAACGCATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAG‑CCCCCcg                                                                                             >  SRR4302533.2953300/1‑92 (MQ=60)
  CAAAGAAAGAATTAACGCATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAG‑CCCCCcg                                                                                             <  SRR4302533.5307980/94‑3 (MQ=60)
   AAAGAAAGAATTAACGCATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAG‑CCCCCcgc                                                                                            >  SRR4302533.2923108/1‑91 (MQ=60)
    AAGAAAGAATTAACGCATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCG                                                                                            >  SRR4302533.2153376/1‑94 (MQ=60)
    AAGAAAGAATTAACGCATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCG                                                                                            >  SRR4302533.1852036/1‑94 (MQ=60)
    AAGAAAGAATTAACGCATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCG                                                                                            >  SRR4302533.1769733/1‑94 (MQ=60)
     AGAAAGAATTAACGCATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGT                                                                                           >  SRR4302533.3246221/1‑94 (MQ=60)
     AGAAAGAATTAACGCATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGT                                                                                           <  SRR4302533.4265807/94‑1 (MQ=60)
     AGAAAGAATTAACGCATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTCTTTTTAATGACAGCCCCCCGCGT                                                                                           >  SRR4302533.4520885/1‑94 (MQ=60)
      GAAAGAATTAACGCATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTC                                                                                          >  SRR4302533.3690835/1‑94 (MQ=60)
      GAAAGAATTAACGCATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTC                                                                                          <  SRR4302533.6079735/94‑1 (MQ=60)
      GAAAGAATTAACGCATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTC                                                                                          >  SRR4302533.5118120/1‑94 (MQ=60)
       AAAGAATTAACGCATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCT                                                                                         >  SRR4302533.317918/1‑94 (MQ=60)
       AAAGAATTAACGCATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCT                                                                                         <  SRR4302533.2513058/94‑1 (MQ=60)
        AAGAATTAACGCATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTT                                                                                        <  SRR4302533.5974953/94‑1 (MQ=60)
        AAGAATTAACGCATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTT                                                                                        >  SRR4302533.2019434/1‑94 (MQ=60)
         AGAATTAACGCATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTG                                                                                       <  SRR4302533.6077649/94‑1 (MQ=60)
          GAATTAACGCATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGA                                                                                      >  SRR4302533.528464/1‑94 (MQ=60)
              TAACGCATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCAC                                                                                  >  SRR4302533.1300215/1‑94 (MQ=60)
              TAACGCATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCAC                                                                                  <  SRR4302533.2351706/94‑1 (MQ=60)
              TAACGCATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCAC                                                                                  >  SRR4302533.1029646/1‑94 (MQ=60)
               AACGCATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACC                                                                                 <  SRR4302533.128720/94‑1 (MQ=60)
               AACGCATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACC                                                                                 >  SRR4302533.3895072/1‑94 (MQ=60)
               AACGCATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACC                                                                                 >  SRR4302533.4740687/1‑94 (MQ=60)
               AACGCATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAG‑CCCCCcgcgtctttgttccc                                                                                >  SRR4302533.1136873/1‑79 (MQ=60)
                  GCATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCT                                                                              >  SRR4302533.4485267/1‑94 (MQ=60)
                   CATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTA                                                                             >  SRR4302533.3011269/1‑94 (MQ=60)
                   CATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTA                                                                             >  SRR4302533.4618477/1‑94 (MQ=60)
                   CATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTA                                                                             <  SRR4302533.5653528/94‑1 (MQ=60)
                   CATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTA                                                                             <  SRR4302533.2684249/94‑1 (MQ=60)
                    ATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTAC                                                                            >  SRR4302533.915450/1‑94 (MQ=60)
                     TCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACA                                                                           >  SRR4302533.2250961/1‑94 (MQ=60)
                     TCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACA                                                                           <  SRR4302533.1346170/94‑1 (MQ=60)
                       GCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATA                                                                         >  SRR4302533.144975/1‑94 (MQ=60)
                          TCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCA                                                                      >  SRR4302533.6177656/1‑94 (MQ=60)
                          TCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCA                                                                      >  SRR4302533.656084/1‑94 (MQ=60)
                           CCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCAT                                                                     <  SRR4302533.5523223/94‑1 (MQ=60)
                             AGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCC                                                                   <  SRR4302533.1888099/94‑1 (MQ=60)
                             AGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCC                                                                   <  SRR4302533.146685/94‑1 (MQ=60)
                              GACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCG                                                                  <  SRR4302533.4297359/94‑1 (MQ=60)
                               ACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGA                                                                 <  SRR4302533.4220983/94‑1 (MQ=60)
                               ACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGA                                                                 >  SRR4302533.4002502/1‑94 (MQ=60)
                               ACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGA                                                                 >  SRR4302533.1864459/1‑94 (MQ=60)
                                CTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAA                                                                <  SRR4302533.4535010/94‑1 (MQ=60)
                                CTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAA                                                                <  SRR4302533.6030289/94‑1 (MQ=60)
                                CTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAA                                                                <  SRR4302533.3795828/94‑1 (MQ=60)
                                CTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAA                                                                >  SRR4302533.5941966/1‑94 (MQ=60)
                                 TGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAG                                                               <  SRR4302533.5503012/94‑1 (MQ=60)
                                 TGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAG                                                               <  SRR4302533.1106138/94‑1 (MQ=60)
                                    TCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTT                                                            <  SRR4302533.1038994/94‑1 (MQ=60)
                                    TCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTT                                                            <  SRR4302533.1547843/94‑1 (MQ=60)
                                    TCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTT                                                            >  SRR4302533.1896662/1‑94 (MQ=60)
                                     CTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCCCCTCTACATGCCATCCGAAGCTTT                                                           <  SRR4302533.3479650/94‑1 (MQ=60)
                                     CTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTT                                                           <  SRR4302533.4725150/94‑1 (MQ=60)
                                     CTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTT                                                           <  SRR4302533.1433234/94‑1 (MQ=60)
                                      TGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTT                                                          <  SRR4302533.5199480/94‑1 (MQ=60)
                                      TGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTT                                                          <  SRR4302533.1359272/94‑1 (MQ=60)
                                      TGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATAACATCCGAAGCTTTT                                                          >  SRR4302533.5366467/1‑94 (MQ=60)
                                      TGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGGCTTGTTCACCTCTACATACCATACGAAAGTTTT                                                          >  SRR4302533.3328628/1‑94 (MQ=60)
                                       GAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTC                                                         <  SRR4302533.5205669/94‑1 (MQ=60)
                                       GAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTC                                                         <  SRR4302533.5930971/94‑1 (MQ=60)
                                        AATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCT                                                        <  SRR4302533.2913222/94‑1 (MQ=60)
                                         ATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTT                                                       <  SRR4302533.6296977/94‑1 (MQ=60)
                                         ATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTT                                                       <  SRR4302533.493662/94‑1 (MQ=60)
                                         ATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTT                                                       <  SRR4302533.3894862/94‑1 (MQ=60)
                                         ATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTT                                                       <  SRR4302533.1429002/94‑1 (MQ=60)
                                            CGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTACTTGCG                                                    <  SRR4302533.5677699/94‑1 (MQ=60)
                                               CGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGGCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTCCGTGT                                                 >  SRR4302533.4121090/1‑94 (MQ=60)
                                                GATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTT                                                >  SRR4302533.3205397/1‑94 (MQ=60)
                                                  TAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCT                                              >  SRR4302533.2392756/1‑94 (MQ=60)
                                                   AATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTT                                             >  SRR4302533.2072132/1‑94 (MQ=60)
                                                    ATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTG                                            <  SRR4302533.4777556/94‑1 (MQ=60)
                                                     TTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGT                                           <  SRR4302533.6239690/94‑1 (MQ=60)
                                                        AGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAG                                        <  SRR4302533.5278740/94‑1 (MQ=60)
                                                        AGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAG                                        <  SRR4302533.2598732/94‑1 (MQ=60)
                                                        AGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAG                                        >  SRR4302533.995344/1‑94 (MQ=60)
                                                        AGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAG                                        <  SRR4302533.5437729/94‑1 (MQ=60)
                                                        AGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAG                                        <  SRR4302533.1173977/94‑1 (MQ=60)
                                                           TGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCT                                     >  SRR4302533.5188396/1‑94 (MQ=60)
                                                            gcgtttaagtttttaTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTT                                    <  SRR4302533.6304313/79‑1 (MQ=60)
                                                            GCTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTT                                    <  SRR4302533.1334652/94‑1 (MQ=60)
                                                             CTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTC                                   >  SRR4302533.3315030/1‑94 (MQ=60)
                                                             CTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTC                                   >  SRR4302533.6214009/1‑94 (MQ=60)
                                                             CTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTC                                   >  SRR4302533.1925123/1‑94 (MQ=60)
                                                             CTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTC                                   <  SRR4302533.2571920/94‑1 (MQ=60)
                                                             CTTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTC                                   <  SRR4302533.314946/94‑1 (MQ=60)
                                                              TTTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCC                                  >  SRR4302533.4888394/1‑94 (MQ=60)
                                                               TTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCT                                 <  SRR4302533.598123/94‑1 (MQ=60)
                                                               TTAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCT                                 <  SRR4302533.2724748/94‑1 (MQ=60)
                                                                TAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTT                                >  SRR4302533.5495855/1‑94 (MQ=60)
                                                                TAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTT                                >  SRR4302533.3058949/1‑94 (MQ=60)
                                                                TAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTT                                >  SRR4302533.5659495/1‑94 (MQ=60)
                                                                TAATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTT                                <  SRR4302533.4166900/94‑1 (MQ=60)
                                                                 AATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTC                               <  SRR4302533.4674150/94‑1 (MQ=60)
                                                                 AATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTC                               <  SRR4302533.947805/94‑1 (MQ=60)
                                                                 AATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTC                               >  SRR4302533.114205/1‑94 (MQ=60)
                                                                 AATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTC                               <  SRR4302533.2583454/94‑1 (MQ=60)
                                                                 AATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTC                               <  SRR4302533.43297/94‑1 (MQ=60)
                                                                  ATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCG                              <  SRR4302533.5373724/94‑1 (MQ=60)
                                                                  ATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCG                              >  SRR4302533.5230080/1‑94 (MQ=60)
                                                                  ATGGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCC                              >  SRR4302533.5214297/1‑94 (MQ=60)
                                                                    TGTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCC                            <  SRR4302533.5176758/94‑1 (MQ=60)
                                                                     GTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCA                           <  SRR4302533.5205689/94‑1 (MQ=60)
                                                                     GTTATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCA                           >  SRR4302533.2244088/1‑94 (MQ=60)
                                                                        ATTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTA                        <  SRR4302533.4206536/94‑1 (MQ=60)
                                                                         TTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTAT                       <  SRR4302533.2169329/94‑1 (MQ=60)
                                                                         TTTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTAT                       <  SRR4302533.5794791/94‑1 (MQ=60)
                                                                          TTTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATC                      <  SRR4302533.3141221/94‑1 (MQ=60)
                                                                           TTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCA                     >  SRR4302533.2554774/1‑94 (MQ=60)
                                                                           TTTTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCA                     <  SRR4302533.3962377/94‑1 (MQ=60)
                                                                             TTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATC                   <  SRR4302533.5240697/94‑1 (MQ=60)
                                                                             TTTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATC                   >  SRR4302533.1564321/1‑94 (MQ=60)
                                                                              TTTAATGACAGCCCCCCGCGTCTTGATCGCCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCA                  <  SRR4302533.6324957/94‑1 (MQ=60)
                                                                              TTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGGTTTCTTCGCCATTATTATCA                  >  SRR4302533.3358235/1‑94 (MQ=60)
                                                                              TTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCA                  <  SRR4302533.5705038/94‑1 (MQ=60)
                                                                              TTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCA                  <  SRR4302533.2737073/94‑1 (MQ=60)
                                                                              TTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCA                  <  SRR4302533.761655/94‑1 (MQ=60)
                                                                              TTTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCA                  >  SRR4302533.1088577/1‑94 (MQ=60)
                                                                               TTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCAC                 <  SRR4302533.6238434/94‑1 (MQ=60)
                                                                               TTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCAC                 <  SRR4302533.1173282/94‑1 (MQ=60)
                                                                               TTAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCAC                 >  SRR4302533.3849379/1‑94 (MQ=60)
                                                                                TAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACG                <  SRR4302533.3030833/94‑1 (MQ=60)
                                                                                TAATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACG                >  SRR4302533.3981422/1‑94 (MQ=60)
                                                                                 AATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGA               >  SRR4302533.5844000/1‑94 (MQ=60)
                                                                                  ATGACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGAT              <  SRR4302533.2926708/94‑1 (MQ=60)
                                                                                    GACAGCCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCC            >  SRR4302533.5120072/1‑94 (MQ=60)
                                                                                       agcCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACA         >  SRR4302533.2725306/4‑94 (MQ=60)
                                                                                       agcCCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACA         <  SRR4302533.4719028/91‑1 (MQ=60)
                                                                                        C‑CCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATA       >  SRR4302533.4897463/1‑94 (MQ=60)
                                                                                        C‑CCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATA       <  SRR4302533.3737335/94‑1 (MQ=60)
                                                                                          CCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAA      >  SRR4302533.387195/1‑94 (MQ=60)
                                                                                          CCCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAA      >  SRR4302533.2889166/1‑94 (MQ=60)
                                                                                            CCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAAT    <  SRR4302533.3337656/94‑1 (MQ=60)
                                                                                            CCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAAT    >  SRR4302533.4407883/1‑94 (MQ=60)
                                                                                            CCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAAT    <  SRR4302533.4274685/94‑1 (MQ=60)
                                                                                            CCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAAT    >  SRR4302533.5300550/1‑94 (MQ=60)
                                                                                             CCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATA   >  SRR4302533.4916913/1‑94 (MQ=60)
                                                                                             CCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATA   >  SRR4302533.3437780/1‑94 (MQ=60)
                                                                                             CCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATA   <  SRR4302533.484585/94‑1 (MQ=60)
                                                                                             CCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATA   >  SRR4302533.5380994/1‑94 (MQ=60)
                                                                                              CGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAA  >  SRR4302533.4305846/1‑94 (MQ=60)
                                                                                              CGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAA  <  SRR4302533.5522430/94‑1 (MQ=60)
                                                                                              |                                                                                             
TGCAAAGAAAGAATTAACGCATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAG‑CCCCCGCGTCTTGATCACCTCTGCATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAA  >  NC_000913/3899864‑3900050

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑