Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,901,321	T→G	A3A (GCA→GCC)	bglB ←	phospho‑beta‑glucosidase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,901,321	0	T	G	100.0%	893.7 / NA	275	A3A (GCA→GCC)	bglB	phospho‑beta‑glucosidase B
Reads supporting (aligned to +/- strand): ref base T (0/0); new base G (130/145); total (130/145)

Only 100 of 278 total aligned reads displayed.

TGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAATGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCGCCGTGCCGTGGGGTAATAC  >  NC_000913/3901231‑3901410

tGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGc                                                                                         >  2:731484/1‑93 (MQ=255)

  aGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCtt                                                                                       <  2:306740/93‑1 (MQ=255)

   ggTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCttt                                                                                      <  1:3872653/93‑1 (MQ=255)

   ggTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCttt                                                                                      <  2:468537/93‑1 (MQ=255)

     tCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCa                                                                                    <  2:834983/93‑1 (MQ=255)

     tCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCa                                                                                    <  1:1578890/93‑1 (MQ=255)

      cGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCAt                                                                                   <  1:2251502/93‑1 (MQ=255)

       gagaTCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATa                                                                                  <  1:5695771/93‑1 (MQ=255)

       gagaTCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATa                                                                                  >  2:1846593/1‑93 (MQ=255)

       gagaTCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATa                                                                                  >  2:3081776/1‑93 (MQ=255)

       gagaTCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATa                                                                                  >  1:2895774/1‑93 (MQ=255)

        agaTCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATaa                                                                                 <  2:3889469/93‑1 (MQ=255)

         gaTCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAAt                                                                                >  1:3176393/1‑93 (MQ=255)

         gaTCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAAt                                                                                >  2:2136180/1‑93 (MQ=255)

          aTCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAAtt                                                                               >  1:6038427/1‑93 (MQ=255)

           tCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTa                                                                              <  1:2705300/93‑1 (MQ=255)

           tCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGGTTCTGGAAAGGCTTTCATAATTa                                                                              >  2:137914/1‑93 (MQ=255)

            cccTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTaa                                                                             <  1:5998685/93‑1 (MQ=255)

             ccTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAAc                                                                            <  2:5452024/93‑1 (MQ=255)

               tttGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTc                                                                          <  2:2308388/93‑1 (MQ=255)

                ttGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTcc                                                                         <  1:4877352/93‑1 (MQ=255)

                   ccATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCttt                                                                      <  1:3146737/93‑1 (MQ=255)

                   ccATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCttt                                                                      >  2:2586372/1‑93 (MQ=255)

                   ccATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCttt                                                                      <  1:4256143/93‑1 (MQ=255)

                    cATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCtttt                                                                     <  2:3478458/93‑1 (MQ=255)

                    cATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCtttt                                                                     <  2:2628513/93‑1 (MQ=255)

                    cATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCtttt                                                                     >  1:1010796/1‑93 (MQ=255)

                    cATCTTCATGCCAGGCACCTTCCTCCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCtttt                                                                     >  2:5100452/1‑93 (MQ=255)

                     aTCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTa                                                                    <  2:893454/93‑1 (MQ=255)

                     aTCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTa                                                                    <  1:31066/93‑1 (MQ=255)

                     aTCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTATCTGGAAAGGCTTTCATAATTAACTCCTTTTa                                                                    <  1:3392520/93‑1 (MQ=255)

                      tCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTAt                                                                   >  2:4992454/1‑93 (MQ=255)

                      tCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTAt                                                                   >  2:442872/1‑93 (MQ=255)

                      tCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTAt                                                                   >  2:337761/1‑93 (MQ=255)

                      tCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTAt                                                                   <  2:1566281/93‑1 (MQ=255)

                      tCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTAt                                                                   >  1:5276713/1‑93 (MQ=255)

                        ttCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGGAAGGCGTTCATAATTAACTTCTTTTATCg                                                                 >  2:1784152/1‑93 (MQ=255)

                        ttCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATct                                                                 >  1:3562362/1‑92 (MQ=255)

                        ttCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCg                                                                 >  2:3334387/1‑93 (MQ=255)

                           cTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTa                                                              <  2:203763/93‑1 (MQ=255)

                           cTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTa                                                              >  2:4677881/1‑93 (MQ=255)

                           cTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTa                                                              <  2:5828788/93‑1 (MQ=255)

                            tGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAg                                                             <  2:663430/93‑1 (MQ=255)

                             gCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGc                                                            >  2:23607/1‑93 (MQ=255)

                                 ggCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAat                                                        >  1:5507154/1‑93 (MQ=255)

                                 ggCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAat                                                        <  2:313825/93‑1 (MQ=255)

                                   cACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAatga                                                      <  2:5539205/93‑1 (MQ=255)

                                   cACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAatga                                                      >  1:296694/1‑93 (MQ=255)

                                   cACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAatga                                                      <  2:2758222/93‑1 (MQ=255)

                                     ccTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAatgatg                                                    <  1:5802630/93‑1 (MQ=255)

                                       ttCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGa                                                  <  2:363304/93‑1 (MQ=255)

                                       ttCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGa                                                  >  1:3777330/1‑93 (MQ=255)

                                        tCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGAt                                                 >  2:3519236/1‑93 (MQ=255)

                                         ccACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATa                                                <  1:250569/93‑1 (MQ=255)

                                          cACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATaa                                               <  1:5353581/93‑1 (MQ=255)

                                           aCCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAAc                                              <  1:5074570/93‑1 (MQ=255)

                                           aCCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAAc                                              <  1:4081057/93‑1 (MQ=255)

                                            ccTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACa                                             <  2:4357428/93‑1 (MQ=255)

                                             cTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAg                                            >  1:3615635/1‑93 (MQ=255)

                                             cTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAg                                            >  2:6132475/1‑93 (MQ=255)

                                               gATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCg                                          <  2:5912127/93‑1 (MQ=255)

                                               gATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCg                                          >  2:4070205/1‑93 (MQ=255)

                                                aTTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCgg                                         <  2:6045751/93‑1 (MQ=255)

                                                  tGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGtt                                       >  2:2838093/1‑93 (MQ=255)

                                                    gCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCa                                     >  1:4118541/1‑93 (MQ=255)

                                                      aGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCAcc                                   <  1:4212489/93‑1 (MQ=255)

                                                      aGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCAcc                                   >  2:1112919/1‑93 (MQ=255)

                                                       gCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCt                                  >  2:3773718/1‑93 (MQ=255)

                                                       gCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCt                                  <  1:1048430/93‑1 (MQ=255)

                                                       gCTGTTGCGCCACCCCAAAGAAAAGTTTCTGGAAAGGCTTGCACAATGAACTCCTTGTATCGTTAGCGAATGACGGATAACAGCGGTTCAccg                                  >  1:6304878/1‑92 (MQ=255)

                                                        cTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTg                                 <  1:5882535/93‑1 (MQ=255)

                                                         tGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTgc                                <  2:4389901/93‑1 (MQ=255)

                                                         tGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTgc                                >  1:6182525/1‑93 (MQ=255)

                                                          gTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTgcg                               >  1:6264368/1‑93 (MQ=255)

                                                           ttGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTgcgc                              <  1:1209121/93‑1 (MQ=255)

                                                                ccACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATc                         <  2:766807/93‑1 (MQ=255)

                                                                 cACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCt                        <  1:5730234/93‑1 (MQ=255)

                                                                  aCCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTg                       >  1:1420037/1‑93 (MQ=255)

                                                                   ccccAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTgc                      >  1:1596009/1‑93 (MQ=255)

                                                                     ccAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTgcgc                    >  2:4233368/1‑93 (MQ=255)

                                                                     ccAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTgcgc                    >  2:4792098/1‑93 (MQ=255)

                                                                       aaaGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCgacg                  >  2:1934543/1‑93 (MQ=255)

                                                                        aaGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCgccgt                 <  1:1687548/93‑1 (MQ=255)

                                                                         aGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCgccgtg                >  1:4813875/1‑93 (MQ=255)

                                                                            aaTGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCgccgtgccg             >  1:2184237/1‑93 (MQ=255)

                                                                            aaTGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCgccgtgccg             >  1:462764/1‑93 (MQ=255)

                                                                             aTGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCgccgtgccgt            <  2:5978437/93‑1 (MQ=255)

                                                                             aTGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCgccgtgccgt            <  2:5178499/93‑1 (MQ=255)

                                                                             aTGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCgccgtgccgt            >  2:3916718/1‑93 (MQ=255)

                                                                              tGTTTCTGGAAAGGCTTTCATAATTAGCTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCGccgtgccgtg           <  1:3348354/93‑1 (MQ=255)

                                                                              tGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCGccgtgccgtg           >  1:2027412/1‑93 (MQ=255)

                                                                                tttCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCGCCGTGCCGTggg         <  2:575455/93‑1 (MQ=255)

                                                                                tttCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCGCCGTGCCGTggg         <  2:6306468/93‑1 (MQ=255)

                                                                                 ttCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCGCCGTGCCGTgggg        <  2:1646411/93‑1 (MQ=255)

                                                                                    tGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATTGATAACAGCGGTTCGCCTGCTCTTATCTGCGCCGTGCCGTGGGGTaa     <  1:4941414/93‑1 (MQ=255)

                                                                                    tGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCGCCGTGCCGTGGGGTaa     <  1:5544364/93‑1 (MQ=255)

                                                                                      gAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCGCCGTGCCGTGGGGTAATa   <  2:56639/93‑1 (MQ=255)

                                                                                       aaaGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCGCCGTGCCGTGGGGTAATAc  <  1:1852182/93‑1 (MQ=255)

                                                                                       aaaGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCGCCGTGCCGTGGGGTAATAc  <  1:864776/93‑1 (MQ=255)

                                                                                       aaaGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCGCCGTGCCGTGGGGTAATAc  <  1:428612/93‑1 (MQ=255)

TGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAATGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCGCCGTGCCGTGGGGTAATAC  >  NC_000913/3901231‑3901410

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

ATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAATGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCGCCGTGCCGTGGGGTAATACGTCC  >  NC_000913/3901228‑3901414

ATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAG                                                                                               <  SRR4302533.6341657/94‑1 (MQ=60)

ATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAG                                                                                               <  SRR4302533.2312433/94‑1 (MQ=60)

   TGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCT                                                                                            <  SRR4302533.2959868/94‑1 (MQ=60)

   TGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCT                                                                                            <  SRR4302533.3787463/94‑1 (MQ=60)

      GGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTC                                                                                         <  SRR4302533.3896290/94‑1 (MQ=60)

      GGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTC                                                                                         <  SRR4302533.2178287/94‑1 (MQ=60)

      GGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTC                                                                                         <  SRR4302533.3277781/94‑1 (MQ=60)

       GTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCA                                                                                        <  SRR4302533.4892376/94‑1 (MQ=60)

       GTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCGTTCA                                                                                        >  SRR4302533.203753/1‑94 (MQ=60)

       GTCGAGATCCCTTCGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCA                                                                                        <  SRR4302533.3627087/94‑1 (MQ=60)

        TCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCAT                                                                                       >  SRR4302533.2460774/1‑94 (MQ=60)

        TCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCAT                                                                                       <  SRR4302533.1588399/94‑1 (MQ=60)

        TCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCAT                                                                                       <  SRR4302533.3275664/94‑1 (MQ=60)

         CGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATA                                                                                      <  SRR4302533.2265422/94‑1 (MQ=60)

         CGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATA                                                                                      >  SRR4302533.2913582/1‑94 (MQ=60)

         CGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATA                                                                                      <  SRR4302533.1941106/94‑1 (MQ=60)

          GAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAA                                                                                     <  SRR4302533.5619999/94‑1 (MQ=60)

          GAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAA                                                                                     <  SRR4302533.5730180/94‑1 (MQ=60)

           AGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAAT                                                                                    >  SRR4302533.3195932/1‑94 (MQ=60)

           AGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAAT                                                                                    >  SRR4302533.5053858/1‑94 (MQ=60)

           AGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAAT                                                                                    <  SRR4302533.5911019/94‑1 (MQ=60)

           AGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAAT                                                                                    <  SRR4302533.3386842/94‑1 (MQ=60)

           AGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAAT                                                                                    >  SRR4302533.4117471/1‑94 (MQ=60)

            GATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATT                                                                                   >  SRR4302533.6075019/1‑94 (MQ=60)

            GATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATT                                                                                   <  SRR4302533.4438445/94‑1 (MQ=60)

             ATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTA                                                                                  >  SRR4302533.4978709/1‑94 (MQ=60)

             ATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTA                                                                                  <  SRR4302533.5380593/94‑1 (MQ=60)

             ATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTA                                                                                  <  SRR4302533.6260032/94‑1 (MQ=60)

              TCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAA                                                                                 <  SRR4302533.2721893/94‑1 (MQ=60)

               CCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAAC                                                                                <  SRR4302533.6035017/94‑1 (MQ=60)

               CCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAAC                                                                                >  SRR4302533.5814147/1‑94 (MQ=60)

                CCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACT                                                                               >  SRR4302533.4804944/1‑94 (MQ=60)

                CCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACT                                                                               <  SRR4302533.4205096/94‑1 (MQ=60)

                 CTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTC                                                                              <  SRR4302533.5462158/94‑1 (MQ=60)

                   TTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCT                                                                            <  SRR4302533.964493/94‑1 (MQ=60)

                   TTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCT                                                                            <  SRR4302533.4906901/94‑1 (MQ=60)

                    TGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTT                                                                           <  SRR4302533.4177106/94‑1 (MQ=60)

                    TGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTT                                                                           >  SRR4302533.1739392/1‑94 (MQ=60)

                     GCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTT                                                                          <  SRR4302533.314280/94‑1 (MQ=60)

                      CCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTT                                                                         <  SRR4302533.4281803/94‑1 (MQ=60)

                      CCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTT                                                                         >  SRR4302533.1016709/1‑94 (MQ=60)

                      CCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTT                                                                         >  SRR4302533.1687021/1‑94 (MQ=60)

                      CCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTT                                                                         <  SRR4302533.3166087/94‑1 (MQ=60)

                        ATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTAT                                                                       >  SRR4302533.5776313/1‑94 (MQ=60)

                        ATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTAT                                                                       >  SRR4302533.5308346/1‑94 (MQ=60)

                        ATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTAT                                                                       <  SRR4302533.31279/94‑1 (MQ=60)

                        ATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTATCTGGAAAGGCTTTCATAATTAACTCCTTTTAT                                                                       <  SRR4302533.3413470/94‑1 (MQ=60)

                         TCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATC                                                                      <  SRR4302533.615029/94‑1 (MQ=60)

                          CTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCT                                                                     >  SRR4302533.3584310/1‑94 (MQ=60)

                          CTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCG                                                                     <  SRR4302533.3160812/94‑1 (MQ=60)

                           TTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGT                                                                    >  SRR4302533.813873/1‑94 (MQ=60)

                            TCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTT                                                                   <  SRR4302533.4337900/94‑1 (MQ=60)

                            TCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTT                                                                   <  SRR4302533.5404720/94‑1 (MQ=60)

                             CCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTA                                                                  >  SRR4302533.4273571/1‑94 (MQ=60)

                              CTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAG                                                                 >  SRR4302533.6147738/1‑94 (MQ=60)

                               TGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGC                                                                <  SRR4302533.3523318/94‑1 (MQ=60)

                                GCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCG                                                               <  SRR4302533.34985/94‑1 (MQ=60)

                                GCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCG                                                               >  SRR4302533.2785425/1‑94 (MQ=60)

                                 CCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGA                                                              >  SRR4302533.1591632/1‑94 (MQ=60)

                                   AGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAAT                                                            >  SRR4302533.1636265/1‑94 (MQ=60)

                                   AGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAAT                                                            >  SRR4302533.5540387/1‑94 (MQ=60)

                                    GGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATG                                                           >  SRR4302533.1065884/1‑94 (MQ=60)

                                     GCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGA                                                          >  SRR4302533.298511/1‑94 (MQ=60)

                                      CACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGAT                                                         >  SRR4302533.5716296/1‑94 (MQ=60)

                                        CCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGG                                                       <  SRR4302533.5837680/94‑1 (MQ=60)

                                         CTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGA                                                      >  SRR4302533.3800477/1‑94 (MQ=60)

                                            CCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAA                                                   <  SRR4302533.252115/94‑1 (MQ=60)

                                             CACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAAC                                                  >  SRR4302533.4294217/1‑94 (MQ=60)

                                             CACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAAC                                                  <  SRR4302533.5385711/94‑1 (MQ=60)

                                              ACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACA                                                 <  SRR4302533.5105244/94‑1 (MQ=60)

                                              ACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACA                                                 <  SRR4302533.4105902/94‑1 (MQ=60)

                                              ACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACA                                                 <  SRR4302533.3042864/94‑1 (MQ=60)

                                               CCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAG                                                <  SRR4302533.2265345/94‑1 (MQ=60)

                                               CCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAG                                                <  SRR4302533.2756877/94‑1 (MQ=60)

                                               CCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAG                                                <  SRR4302533.3076716/94‑1 (MQ=60)

                                               CCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAG                                                >  SRR4302533.3637879/1‑94 (MQ=60)

                                                 TGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCG                                              <  SRR4302533.2569359/94‑1 (MQ=60)

                                                  GATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGG                                             >  SRR4302533.6047486/1‑94 (MQ=60)

                                                   ATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGT                                            >  SRR4302533.1486936/1‑94 (MQ=60)

                                                   ATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGT                                            >  SRR4302533.1701050/1‑94 (MQ=60)

                                                   ATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGT                                            <  SRR4302533.5132752/94‑1 (MQ=60)

                                                      GGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCA                                         <  SRR4302533.2577342/94‑1 (MQ=60)

                                                      GGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCA                                         <  SRR4302533.3186117/94‑1 (MQ=60)

                                                      GGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCA                                         >  SRR4302533.4143556/1‑94 (MQ=60)

                                                        CAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACC                                       >  SRR4302533.517482/1‑94 (MQ=60)

                                                        CAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACC                                       <  SRR4302533.4961013/94‑1 (MQ=60)

                                                         AGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCT                                      <  SRR4302533.4237971/94‑1 (MQ=60)

                                                         AGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCT                                      <  SRR4302533.1355587/94‑1 (MQ=60)

                                                         AGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCT                                      >  SRR4302533.4860216/1‑94 (MQ=60)

                                                         AGCTGTTGCGCCACCCCAAAGAAAAGTTTCTGGAAAGGCTTGCACAATGAACTCCTTGTATCGTTAGCGAATGACGGATAACAGCGGTTCACCG                                      >  SRR4302533.6342595/1‑94 (MQ=60)

                                                          GCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTG                                     <  SRR4302533.1054591/94‑1 (MQ=60)

                                                           CTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGC                                    <  SRR4302533.5918085/94‑1 (MQ=60)

                                                           CTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGC                                    >  SRR4302533.6322696/1‑94 (MQ=60)

                                                           CTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGC                                    >  SRR4302533.6219650/1‑94 (MQ=60)

                                                            TGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCG                                   >  SRR4302533.3589015/1‑94 (MQ=60)

                                                            TGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCG                                   >  SRR4302533.6301809/1‑94 (MQ=60)

                                                            TGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCG                                   <  SRR4302533.653536/94‑1 (MQ=60)

                                                            TGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCG                                   >  SRR4302533.6039604/1‑94 (MQ=60)

                                                              TTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCT                                 >  SRR4302533.1467814/1‑94 (MQ=60)

                                                              TTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCT                                 <  SRR4302533.1216382/94‑1 (MQ=60)

                                                                 CGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTAT                              <  SRR4302533.6269454/94‑1 (MQ=60)

                                                                 CGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTAT                              >  SRR4302533.5965505/1‑94 (MQ=60)

                                                                    CACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTG                           <  SRR4302533.5764139/94‑1 (MQ=60)

                                                                    CACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTG                           <  SRR4302533.5764835/94‑1 (MQ=60)

                                                                    CACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTG                           >  SRR4302533.1428628/1‑94 (MQ=60)

                                                                     ACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGC                          >  SRR4302533.1108900/1‑94 (MQ=60)

                                                                     ACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGC                          >  SRR4302533.5335937/1‑94 (MQ=60)

                                                                     ACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGC                          >  SRR4302533.1605668/1‑94 (MQ=60)

                                                                       CCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCGC                        >  SRR4302533.3934111/1‑94 (MQ=60)

                                                                         CAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCGCCG                      >  SRR4302533.4241391/1‑94 (MQ=60)

                                                                          AAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCGCCGT                     <  SRR4302533.3188929/94‑1 (MQ=60)

                                                                          AAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCGCCGT                     >  SRR4302533.2445846/1‑94 (MQ=60)

                                                                          AAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCGCCGT                     >  SRR4302533.1272121/1‑94 (MQ=60)

                                                                          AAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCGCCGT                     >  SRR4302533.4044007/1‑94 (MQ=60)

                                                                           AAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCGCCGTG                    <  SRR4302533.1697775/94‑1 (MQ=60)

                                                                           AAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCGCCGTG                    >  SRR4302533.4843050/1‑94 (MQ=60)

                                                                              AAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCGCCGTGCCG                 >  SRR4302533.6016139/1‑94 (MQ=60)

                                                                              AAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCGCCGTGCCG                 >  SRR4302533.2197701/1‑94 (MQ=60)

                                                                              AAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCGCCGTGCCG                 >  SRR4302533.465522/1‑94 (MQ=60)

                                                                                ATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCGCCGTGCCGTG               >  SRR4302533.212969/1‑94 (MQ=60)

                                                                                ATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCGCCGTGCCGTG               >  SRR4302533.2039930/1‑94 (MQ=60)

                                                                                 TGTTTCTGGAAAGGCTTTCATAATTAGCTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCGCCGTGCCGTGG              <  SRR4302533.3369042/94‑1 (MQ=60)

                                                                                    TTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCGCCGTGCCGTGGGGT           <  SRR4302533.1016902/94‑1 (MQ=60)

                                                                                     TCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCGCCGTGCCGTGGGGTA          >  SRR4302533.5556869/1‑94 (MQ=60)

                                                                                     TCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCGCCGTGCCGTGGGGTA          <  SRR4302533.3832896/94‑1 (MQ=60)

                                                                                      CTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCGCCGTGCCGTGGGGTAA         >  SRR4302533.4120543/1‑94 (MQ=60)

                                                                                       TGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATTGATAACAGCGGTTCGCCTGCTCTTATCTGCGCCGTGCCGTGGGGTAAT        <  SRR4302533.4971327/94‑1 (MQ=60)

                                                                                       TGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCGCCGTGCCGTGGGGTAAT        <  SRR4302533.5577849/94‑1 (MQ=60)

                                                                                        GGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCGCCGTGCCGTGGGGTAATA       >  SRR4302533.4822140/1‑94 (MQ=60)

                                                                                        GGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCGCCGTGCCGTGGGGTAATA       <  SRR4302533.3654642/94‑1 (MQ=60)

                                                                                         GAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCGCCGTGCCGTGGGGTAATAC      <  SRR4302533.5762172/94‑1 (MQ=60)

                                                                                          AAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCGCCGTGCCGTGGGGTAATACG     <  SRR4302533.1863477/94‑1 (MQ=60)

                                                                                          AAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCGCCGTGCCGTGGGGTAATACG     <  SRR4302533.431168/94‑1 (MQ=60)

                                                                                          AAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCGCCGTGCCGTGGGGTAATACG     <  SRR4302533.869841/94‑1 (MQ=60)

                                                                                            AGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCGCCGTGCCGTGGGGTAATACGTC   >  SRR4302533.174869/1‑94 (MQ=60)

                                                                                            AGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCGCCGTGCCGTGGGGTAATACGTC   <  SRR4302533.78729/94‑1 (MQ=60)

                                                                                            AGGCTTTCATAATTAACTCCTTTTATCGTTAGCAAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCGCCGTGCCGTGGGGTAATACGTC   >  SRR4302533.5961386/1‑94 (MQ=60)

                                                                                             GGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCGCCGTGCCGTGGGGTAATACGTCC  <  SRR4302533.3644451/94‑1 (MQ=60)

ATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAATGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCGCCGTGCCGTGGGGTAATACGTCC  >  NC_000913/3901228‑3901414

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`