Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,902,432 G→A P265L (CCG→CTG)  bglF ← PTS system beta‑glucosides‑specific enzyme IIABC component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,902,4320GA99.3% 1533.6 / ‑3.9 421P265L (CCG→CTG) bglFPTS system beta‑glucosides‑specific enzyme IIABC component
Reads supporting (aligned to +/- strand):  ref base G (1/2);  new base A (250/168);  total (251/170)
Fisher's exact test for biased strand distribution p-value = 5.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 440 total aligned reads displayed.

CCGCCCATTACCGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCGGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAACCACGCA  >  NC_000913/3902342‑3902521
                                                                                          |                                                                                         
ccgccCATTACCGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATagcagc                                                                                         >  1:1189644/1‑93 (MQ=255)
ccgccCATTACCGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATagcagc                                                                                         <  2:2678827/93‑1 (MQ=255)
 cgccCATTACCGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGcc                                                                                        <  2:7578659/93‑1 (MQ=255)
  gccCATTACCGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGccc                                                                                       >  1:10040738/1‑93 (MQ=255)
  gccCATTACCGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGccc                                                                                       >  2:949999/1‑93 (MQ=255)
   cccATTACCGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGcccc                                                                                      >  2:9798660/1‑93 (MQ=255)
     cATTACCGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCccac                                                                                    >  1:8495881/1‑93 (MQ=255)
     cATTACCGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCccac                                                                                    <  2:9123845/93‑1 (MQ=255)
     cATTACCGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCccac                                                                                    <  1:5825142/93‑1 (MQ=255)
     cATTACCGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCccac                                                                                    >  1:18728/1‑93 (MQ=255)
      aTTACCGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCccacc                                                                                   >  2:8086712/1‑93 (MQ=255)
       ttACCGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCccacca                                                                                  >  2:1592308/1‑93 (MQ=255)
       ttACCGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCccacca                                                                                  >  2:7196266/1‑93 (MQ=255)
       ttACCGCGCCCGCAAATCCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCccacca                                                                                  >  2:8402806/1‑93 (MQ=255)
        tACCGCGCCAGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACcag                                                                                 >  1:4546030/1‑93 (MQ=255)
            gcgcCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGa                                                                             <  1:7340439/93‑1 (MQ=255)
            gcgcCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGa                                                                             >  1:1337262/1‑93 (MQ=255)
             cgcCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGaa                                                                            <  1:3403300/93‑1 (MQ=255)
             cgcCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGaa                                                                            >  1:2930859/1‑93 (MQ=255)
              gcCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGaaa                                                                           <  2:3813770/93‑1 (MQ=255)
              gcCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGaaa                                                                           <  1:5300234/93‑1 (MQ=255)
               cccGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAg                                                                          <  2:5531910/93‑1 (MQ=255)
               cccGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAg                                                                          >  1:9660239/1‑93 (MQ=255)
                ccGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAgg                                                                         >  1:8886947/1‑93 (MQ=255)
                 cGCAGATGCAGGATCCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGt                                                                        <  1:7782604/93‑1 (MQ=255)
                 cGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGt                                                                        <  2:4094323/93‑1 (MQ=255)
                  gCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTg                                                                       >  1:6499679/1‑93 (MQ=255)
                   cAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGa                                                                      <  2:1245295/93‑1 (MQ=255)
                    aaaTGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGAc                                                                     <  1:9781280/93‑1 (MQ=255)
                    aaaTGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGAc                                                                     <  1:7589720/93‑1 (MQ=255)
                      aTGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACgg                                                                   <  1:7447588/93‑1 (MQ=255)
                       tGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACggg                                                                  >  1:5010354/1‑93 (MQ=255)
                       tGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACggg                                                                  >  2:1771346/1‑93 (MQ=255)
                          aGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGgtgt                                                               <  2:8901018/93‑1 (MQ=255)
                          aGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGgtgt                                                               <  1:2223784/93‑1 (MQ=255)
                           ggAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGtgtg                                                              >  1:995871/1‑93 (MQ=255)
                           ggAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGtgtg                                                              >  1:3878079/1‑93 (MQ=255)
                            gAACCGGCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGa                                                             <  1:793638/93‑1 (MQ=255)
                               ccGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATaa                                                          >  2:6050259/1‑93 (MQ=255)
                                  ccTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCa                                                       <  2:4905630/93‑1 (MQ=255)
                                  ccTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCa                                                       >  2:8532313/1‑93 (MQ=255)
                                    tGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATc                                                     >  1:6776832/1‑93 (MQ=255)
                                    tGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATc                                                     >  1:164025/1‑93 (MQ=255)
                                    tGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATc                                                     <  2:2868816/93‑1 (MQ=255)
                                    tGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATc                                                     >  1:8673015/1‑93 (MQ=255)
                                    tGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATc                                                     >  1:241893/1‑93 (MQ=255)
                                    tGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATc                                                     >  2:6298502/1‑93 (MQ=255)
                                     gATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCa                                                    >  2:3558054/1‑93 (MQ=255)
                                      aTAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAg                                                   >  1:4219073/1‑93 (MQ=255)
                                       tAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGa                                                  <  2:7430293/93‑1 (MQ=255)
                                         aaGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACa                                                <  1:1922333/93‑1 (MQ=255)
                                          aGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACAt                                               <  2:612075/93‑1 (MQ=255)
                                           gCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATa                                              >  1:3565905/1‑93 (MQ=255)
                                           accAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATa                                              <  1:6011352/92‑1 (MQ=255)
                                            ccAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAg                                             <  1:6811154/93‑1 (MQ=255)
                                             cAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGc                                            >  2:9057225/1‑93 (MQ=255)
                                             cAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGc                                            >  2:5681446/1‑93 (MQ=255)
                                             cAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGc                                            <  1:6254357/93‑1 (MQ=255)
                                              agagATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCa                                           >  2:2391753/1‑93 (MQ=255)
                                              agagATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCa                                           <  1:6659211/93‑1 (MQ=255)
                                               gagaTAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCaa                                          <  2:6962400/93‑1 (MQ=255)
                                               gagaTAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCaa                                          >  1:2523828/1‑93 (MQ=255)
                                                agaTAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCaag                                         >  1:2006825/1‑92 (MQ=255)
                                                 gaTAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATg                                        >  2:9210443/1‑93 (MQ=255)
                                                 gaTAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATg                                        <  2:5336342/93‑1 (MQ=255)
                                                  aTAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATgg                                       >  2:3342309/1‑93 (MQ=255)
                                                   tAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGgt                                      >  2:6586198/1‑93 (MQ=255)
                                                   tAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGgt                                      >  1:9358899/1‑93 (MQ=255)
                                                       cggcggCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAACGGTGTga                                  <  1:308651/93‑1 (MQ=255)
                                                         gcggcAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTgaag                                >  2:6736539/1‑93 (MQ=255)
                                                          cggcAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTgaaga                               <  1:4473965/93‑1 (MQ=255)
                                                          cggcAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTgaaga                               <  2:2488386/93‑1 (MQ=255)
                                                           ggcAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTgaagaa                              >  1:3682081/1‑93 (MQ=255)
                                                            gcAATCAGTTCGCTTATCCAGGTTGATAGCGGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGaaa                             >  1:3936591/1‑93 (MQ=255)
                                                            gcAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGaaa                             <  1:5703485/93‑1 (MQ=255)
                                                              aaTCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAAtt                           >  1:5099568/1‑93 (MQ=255)
                                                               aTCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAAttt                          >  2:2044192/1‑93 (MQ=255)
                                                               aTCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAAttt                          >  1:9411993/1‑93 (MQ=255)
                                                               aTCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAAttt                          >  2:3060655/1‑93 (MQ=255)
                                                                tCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAAtttt                         >  2:5043084/1‑93 (MQ=255)
                                                                tCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAAtttt                         >  2:3988729/1‑93 (MQ=255)
                                                                tCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAAtttt                         >  1:4078303/1‑93 (MQ=255)
                                                                 cAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAAttttt                        <  2:2277506/93‑1 (MQ=255)
                                                                  aGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTg                       >  2:3220708/1‑93 (MQ=255)
                                                                    ttCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCANTGGTGTGAAGAAATTTTTGAt                     >  1:7144861/1‑93 (MQ=255)
                                                                    ttCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGAt                     <  2:7027551/93‑1 (MQ=255)
                                                                     tCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGAtt                    <  1:8170114/93‑1 (MQ=255)
                                                                       gCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGc                  >  1:8345424/1‑93 (MQ=255)
                                                                          tATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGa               >  1:5520288/1‑93 (MQ=255)
                                                                           aTCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGAc              >  2:4526359/1‑93 (MQ=255)
                                                                             ccAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACgg            <  2:1157104/93‑1 (MQ=255)
                                                                               aGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTa          <  1:47955/93‑1 (MQ=255)
                                                                                ggTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCATTGGTGTGAAGAAATTTTTGATTGCCGACGGTac         >  2:8113954/1‑92 (MQ=255)
                                                                                ggTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTaa         <  2:6510743/93‑1 (MQ=255)
                                                                                ggTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTaa         <  2:2111017/93‑1 (MQ=255)
                                                                                 gTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAAc        >  1:8786239/1‑93 (MQ=255)
                                                                                 gTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAAc        >  1:6965036/1‑93 (MQ=255)
                                                                                  ttGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAAcc       <  1:2881950/93‑1 (MQ=255)
                                                                                  ttGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAAcc       >  2:1964872/1‑93 (MQ=255)
                                                                                       agcagcCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAACCACGCa  <  2:7943124/93‑1 (MQ=255)
                                                                                          |                                                                                         
CCGCCCATTACCGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCGGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAACCACGCA  >  NC_000913/3902342‑3902521

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 246 total aligned reads displayed.
AAGCCGCCCATTACCGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCGGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAACCACGCATTAA  >  NC_000913/3902339‑3902525
                                                                                             |                                                                                             
AAGCCGCCCATTACCGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCA                                                                                               <  SRR4302540.1705511/94‑1 (MQ=60)
AAGCCGCCCATTACCGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCA                                                                                               <  SRR4302540.3888464/94‑1 (MQ=60)
 ATCCGCCCATTACCGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAG                                                                                              <  SRR4302540.7955574/94‑1 (MQ=60)
  GCCGCCCATTACCGCGCCCGCNAATGCAGGAACCGCCTGATAAAACCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGC                                                                                             <  SRR4302540.6518208/94‑1 (MQ=60)
  GCCGCCCATTACCGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGC                                                                                             <  SRR4302540.8619335/94‑1 (MQ=60)
  ACCGCCCATTACCGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGC                                                                                             <  SRR4302540.3541736/94‑1 (MQ=60)
    CGCCCATTACCGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCC                                                                                           >  SRR4302540.10157486/1‑94 (MQ=60)
    CGCCCATTACCGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCC                                                                                           >  SRR4302540.5927965/1‑94 (MQ=60)
    CGCCCATTACCGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCC                                                                                           >  SRR4302540.313787/1‑94 (MQ=60)
     GCCCATTACCGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCC                                                                                          <  SRR4302540.5168064/94‑1 (MQ=60)
       CCATTACCGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCAC                                                                                        >  SRR4302540.18930/1‑94 (MQ=60)
       CCATTACCGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCAC                                                                                        >  SRR4302540.8594603/1‑94 (MQ=60)
        CATTACCGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATTCAGGTTGATAGGAGCCCCACC                                                                                       >  SRR4302540.6642513/1‑94 (MQ=60)
        CATTACCGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACC                                                                                       >  SRR4302540.6642512/1‑94 (MQ=60)
        CATTACCGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACC                                                                                       <  SRR4302540.5889540/94‑1 (MQ=60)
        CATTACCGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACC                                                                                       <  SRR4302540.6494674/94‑1 (MQ=60)
        CATTACCGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACC                                                                                       >  SRR4302540.4532754/1‑94 (MQ=60)
         ATTACCGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCA                                                                                      >  SRR4302540.2544621/1‑94 (MQ=60)
         ATTACCGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCA                                                                                      <  SRR4302540.117371/94‑1 (MQ=60)
          TTACCGCGCCAGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAG                                                                                     >  SRR4302540.4595218/1‑94 (MQ=60)
           TACCGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGC                                                                                    <  SRR4302540.6903512/94‑1 (MQ=60)
           TACCGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGC                                                                                    >  SRR4302540.2893975/1‑94 (MQ=60)
           TACCGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGC                                                                                    <  SRR4302540.6837395/94‑1 (MQ=60)
            ACCGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCA                                                                                   <  SRR4302540.7247565/94‑1 (MQ=60)
              CGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGA                                                                                 <  SRR4302540.8135295/94‑1 (MQ=60)
              CGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGA                                                                                 >  SRR4302540.1351262/1‑94 (MQ=60)
              CGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGA                                                                                 >  SRR4302540.9477198/1‑94 (MQ=60)
               GCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAA                                                                                <  SRR4302540.7422350/94‑1 (MQ=60)
               GCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAA                                                                                >  SRR4302540.2963004/1‑94 (MQ=60)
                CGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAA                                                                               <  SRR4302540.4220900/94‑1 (MQ=60)
                CGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAA                                                                               <  SRR4302540.3440401/94‑1 (MQ=60)
                 GCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAG                                                                              <  SRR4302540.5358225/94‑1 (MQ=60)
                 GCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAG                                                                              <  SRR4302540.5358224/94‑1 (MQ=60)
                 GCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAG                                                                              <  SRR4302540.6213435/94‑1 (MQ=60)
                 GCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAG                                                                              >  SRR4302540.9773951/1‑94 (MQ=60)
                  CCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGG                                                                             <  SRR4302540.6581321/94‑1 (MQ=60)
                  CCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGG                                                                             >  SRR4302540.8989832/1‑94 (MQ=60)
                  CCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGG                                                                             <  SRR4302540.7841011/94‑1 (MQ=60)
                   CCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGT                                                                            >  SRR4302540.9284155/1‑94 (MQ=60)
                   CCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCAACAGCAGAAAGGG                                                                            >  SRR4302540.1932449/1‑94 (MQ=60)
                    CGCAGATGCAGGATCCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTG                                                                           <  SRR4302540.7873574/94‑1 (MQ=60)
                    CGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCCCCAGCAGAAAGGTG                                                                           >  SRR4302540.2848303/1‑94 (MQ=60)
                    CGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTG                                                                           <  SRR4302540.3896510/94‑1 (MQ=60)
                      CAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGAC                                                                         <  SRR4302540.223741/94‑1 (MQ=60)
                       AAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACG                                                                        <  SRR4302540.9895913/94‑1 (MQ=60)
                       AAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACG                                                                        <  SRR4302540.7674966/94‑1 (MQ=60)
                         ATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGG                                                                      >  SRR4302540.5064608/1‑94 (MQ=60)
                         ATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGG                                                                      <  SRR4302540.4697992/94‑1 (MQ=60)
                         ATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGG                                                                      <  SRR4302540.7530582/94‑1 (MQ=60)
                          TGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGT                                                                     >  SRR4302540.8346428/1‑94 (MQ=60)
                          TGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGT                                                                     <  SRR4302540.5144330/94‑1 (MQ=60)
                          TGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGG                                                                     >  SRR4302540.3627603/1‑94 (MQ=60)
                           GCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTG                                                                    >  SRR4302540.10045025/1‑94 (MQ=60)
                            CAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGggg                                                                   >  SRR4302540.1029226/1‑91 (MQ=60)
                            CAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGT                                                                   <  SRR4302540.2489052/94‑1 (MQ=60)
                            CAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTAGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGcgg                                                                   >  SRR4302540.9521170/1‑91 (MQ=60)
                             AGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTG                                                                  >  SRR4302540.5635546/1‑94 (MQ=60)
                             AGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTG                                                                  >  SRR4302540.9232534/1‑94 (MQ=60)
                             AGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTG                                                                  >  SRR4302540.1006011/1‑94 (MQ=60)
                             AGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAACAGCCCCACAAGCAGAAAGGgcacgggggcg                                                                  >  SRR4302540.2948803/1‑83 (MQ=60)
                              GGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGA                                                                 <  SRR4302540.3162622/94‑1 (MQ=60)
                               GAACCGGCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGAT                                                                <  SRR4302540.802066/94‑1 (MQ=60)
                               GAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGAT                                                                <  SRR4302540.802065/94‑1 (MQ=60)
                               GAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGAT                                                                <  SRR4302540.712496/94‑1 (MQ=60)
                                   CGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACC                                                            >  SRR4302540.610279/1‑94 (MQ=60)
                                    GCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTAGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCA                                                           >  SRR4302540.3961863/1‑94 (MQ=60)
                                      CTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATC                                                         >  SRR4302540.4867413/1‑94 (MQ=60)
                                      CTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATC                                                         >  SRR4302540.8773757/1‑94 (MQ=60)
                                      CTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATC                                                         >  SRR4302540.8791505/1‑94 (MQ=60)
                                      CTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATC                                                         <  SRR4302540.9593598/94‑1 (MQ=60)
                                      CTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATC                                                         >  SRR4302540.165668/1‑94 (MQ=60)
                                      CTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATC                                                         >  SRR4302540.1885570/1‑94 (MQ=60)
                                      CTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATC                                                         >  SRR4302540.6851741/1‑94 (MQ=60)
                                      CTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATC                                                         >  SRR4302540.2429876/1‑94 (MQ=60)
                                       TGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCA                                                        <  SRR4302540.22310/94‑1 (MQ=60)
                                        GATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAG                                                       >  SRR4302540.4265003/1‑94 (MQ=60)
                                        GATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAG                                                       <  SRR4302540.2993044/94‑1 (MQ=60)
                                        GATAAAGCCAGAGATAACCGGCGGCAAGCAGTGCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAG                                                       <  SRR4302540.2993043/94‑1 (MQ=60)
                                         ATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGA                                                      >  SRR4302540.4904242/1‑94 (MQ=60)
                                          TAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGAC                                                     <  SRR4302540.7037856/94‑1 (MQ=60)
                                          TAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGAC                                                     >  SRR4302540.1782067/1‑94 (MQ=60)
                                            AAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACAT                                                   <  SRR4302540.8138937/94‑1 (MQ=60)
                                            AAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACAT                                                   <  SRR4302540.1943294/94‑1 (MQ=60)
                                             AGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATA                                                  >  SRR4302540.3604858/1‑94 (MQ=60)
                                              GCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAG                                                 >  SRR4302540.191408/1‑94 (MQ=60)
                                              GCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAG                                                 <  SRR4302540.351896/94‑1 (MQ=60)
                                              ACCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAG                                                 <  SRR4302540.6078015/94‑1 (MQ=60)
                                               CCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGC                                                <  SRR4302540.6886516/94‑1 (MQ=60)
                                               CCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGC                                                <  SRR4302540.6886519/94‑1 (MQ=60)
                                                CAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCA                                               >  SRR4302540.4018470/1‑94 (MQ=60)
                                                CAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCA                                               >  SRR4302540.5999484/1‑94 (MQ=60)
                                                CAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCA                                               <  SRR4302540.2530106/94‑1 (MQ=60)
                                                 AGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAA                                              >  SRR4302540.7532876/1‑94 (MQ=60)
                                                 AGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAA                                              <  SRR4302540.6732841/94‑1 (MQ=60)
                                                 AGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAA                                              >  SRR4302540.2551074/1‑94 (MQ=60)
                                                 AGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAA                                              >  SRR4302540.576927/1‑94 (MQ=60)
                                                 AGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAA                                              <  SRR4302540.4509612/94‑1 (MQ=60)
                                                  GAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAAG                                             >  SRR4302540.2028538/1‑94 (MQ=60)
                                                   AGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATG                                            <  SRR4302540.5824993/94‑1 (MQ=60)
                                                   AGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATG                                            >  SRR4302540.7030640/1‑94 (MQ=60)
                                                    GATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGG                                           <  SRR4302540.3930605/94‑1 (MQ=60)
                                                     ATAAGCGGCGGCAATTAGTGCGCTTATCCAGGTGGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGT                                          <  SRR4302540.1906060/94‑1 (MQ=60)
                                                     ATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGT                                          <  SRR4302540.7929432/94‑1 (MQ=60)
                                                     ATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGT                                          >  SRR4302540.4805876/1‑94 (MQ=60)
                                                     ATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGT                                          >  SRR4302540.9470358/1‑94 (MQ=60)
                                                     ATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGT                                          <  SRR4302540.2282973/94‑1 (MQ=60)
                                                       AACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGT                                        <  SRR4302540.10057425/94‑1 (MQ=60)
                                                       AACCGGCGGCAATCAATTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGT                                        <  SRR4302540.4874169/94‑1 (MQ=60)
                                                         CCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGA                                      <  SRR4302540.5123034/94‑1 (MQ=60)
                                                          CGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAA                                     >  SRR4302540.4264200/1‑94 (MQ=60)
                                                          CGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAA                                     >  SRR4302540.3914539/1‑94 (MQ=60)
                                                          CGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAACGGTGTGAA                                     <  SRR4302540.311710/94‑1 (MQ=60)
                                                           GGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAG                                    >  SRR4302540.7579690/1‑94 (MQ=60)
                                                           GGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAG                                    <  SRR4302540.9778311/94‑1 (MQ=60)
                                                           GGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAG                                    <  SRR4302540.7228612/94‑1 (MQ=60)
                                                            GCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGA                                   <  SRR4302540.3712477/94‑1 (MQ=60)
                                                             CGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAA                                  <  SRR4302540.4522492/94‑1 (MQ=60)
                                                             CGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAA                                  >  SRR4302540.3722322/1‑94 (MQ=60)
                                                              GGCAATCAGTTCGCTTATCCAGGTTGATAGCGGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAA                                 >  SRR4302540.3979133/1‑94 (MQ=60)
                                                              GGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAA                                 >  SRR4302540.5691617/1‑94 (MQ=60)
                                                              GGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAA                                 >  SRR4302540.4056977/1‑94 (MQ=60)
                                                              GGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAA                                 >  SRR4302540.1960263/1‑94 (MQ=60)
                                                              GGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAA                                 >  SRR4302540.1960264/1‑94 (MQ=60)
                                                               GCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAAT                                <  SRR4302540.7641372/94‑1 (MQ=60)
                                                               GCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAAT                                <  SRR4302540.5766284/94‑1 (MQ=60)
                                                                CAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATT                               >  SRR4302540.5154755/1‑94 (MQ=60)
                                                                CAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATT                               >  SRR4302540.8935195/1‑94 (MQ=60)
                                                                CAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATT                               <  SRR4302540.1331539/94‑1 (MQ=60)
                                                                CAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATT                               >  SRR4302540.817811/1‑94 (MQ=60)
                                                                 AATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTT                              >  SRR4302540.9523898/1‑94 (MQ=60)
                                                                 AATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTT                              <  SRR4302540.5785536/94‑1 (MQ=60)
                                                                 AATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTT                              >  SRR4302540.6009322/1‑94 (MQ=60)
                                                                 AATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTT                              >  SRR4302540.9687736/1‑94 (MQ=60)
                                                                 AATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTT                              >  SRR4302540.8641364/1‑94 (MQ=60)
                                                                 AATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTT                              <  SRR4302540.2379334/94‑1 (MQ=60)
                                                                 AATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTT                              >  SRR4302540.8840778/1‑94 (MQ=60)
                                                                 AATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTT                              <  SRR4302540.6739361/94‑1 (MQ=60)
                                                                 AATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTT                              >  SRR4302540.5502665/1‑94 (MQ=60)
                                                                 AATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTT                              >  SRR4302540.8621671/1‑94 (MQ=60)
                                                                  ATCAGTTTGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTT                             >  SRR4302540.4122277/1‑94 (MQ=60)
                                                                  ATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTT                             >  SRR4302540.8301443/1‑94 (MQ=60)
                                                                  ATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTT                             >  SRR4302540.7683262/1‑94 (MQ=60)
                                                                  ATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTT                             >  SRR4302540.4122279/1‑94 (MQ=60)
                                                                   TCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTT                            <  SRR4302540.1235033/94‑1 (MQ=60)
                                                                    CAGTTCGCTTATCCAGGTTGTTAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTG                           >  SRR4302540.2556847/1‑94 (MQ=60)
                                                                    CAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTG                           <  SRR4302540.530094/94‑1 (MQ=60)
                                                                    CAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTG                           >  SRR4302540.1456130/1‑94 (MQ=60)
                                                                    CAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTG                           >  SRR4302540.2354216/1‑94 (MQ=60)
                                                                    CAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTG                           >  SRR4302540.4387300/1‑94 (MQ=60)
                                                                      GTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCANTGGTGTGAAGAAATTTTTGAT                         >  SRR4302540.7224470/1‑94 (MQ=60)
                                                                       TTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATT                        <  SRR4302540.7706696/94‑1 (MQ=60)
                                                                       TTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATT                        >  SRR4302540.5496989/1‑94 (MQ=60)
                                                                       TTCGCTTACCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATT                        <  SRR4302540.3953952/94‑1 (MQ=60)
                                                                       GTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATT                        <  SRR4302540.8730130/94‑1 (MQ=60)
                                                                        TCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTG                       <  SRR4302540.1389695/94‑1 (MQ=60)
                                                                        TCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTG                       <  SRR4302540.8264998/94‑1 (MQ=60)
                                                                         CGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGC                      >  SRR4302540.8442286/1‑94 (MQ=60)
                                                                          GCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCC                     <  SRR4302540.8425805/94‑1 (MQ=60)
                                                                           CTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCG                    >  SRR4302540.7497219/1‑94 (MQ=60)
                                                                           CTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCG                    >  SRR4302540.4574382/1‑94 (MQ=60)
                                                                           CTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCG                    >  SRR4302540.6845844/1‑94 (MQ=60)
                                                                            TTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGA                   >  SRR4302540.5581084/1‑94 (MQ=60)
                                                                            TTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGA                   >  SRR4302540.7516929/1‑94 (MQ=60)
                                                                             TATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGAC                  <  SRR4302540.3644067/94‑1 (MQ=60)
                                                                             TATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGAC                  <  SRR4302540.1544476/94‑1 (MQ=60)
                                                                              ATCCAGGTTGATAGCGGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACGATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACG                 <  SRR4302540.6145396/94‑1 (MQ=60)
                                                                              ATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACG                 >  SRR4302540.378719/1‑94 (MQ=60)
                                                                              ATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACG                 >  SRR4302540.9641949/1‑94 (MQ=60)
                                                                              ATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACG                 >  SRR4302540.4993407/1‑94 (MQ=60)
                                                                                CCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAGGAAATTTTTGATTGCCGACGGT               <  SRR4302540.6423147/94‑1 (MQ=60)
                                                                                CCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGT               <  SRR4302540.6423146/94‑1 (MQ=60)
                                                                                 CAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTA              <  SRR4302540.6203344/94‑1 (MQ=60)
                                                                                  AGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAA             <  SRR4302540.947735/94‑1 (MQ=60)
                                                                                  AGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAA             >  SRR4302540.4656955/1‑94 (MQ=60)
                                                                                  AGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAA             >  SRR4302540.7469382/1‑94 (MQ=60)
                                                                                  AGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAA             >  SRR4302540.131020/1‑94 (MQ=60)
                                                                                  AGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAA             >  SRR4302540.2209076/1‑94 (MQ=60)
                                                                                  AGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAA             >  SRR4302540.8921902/1‑94 (MQ=60)
                                                                                  AGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAA             <  SRR4302540.3549240/94‑1 (MQ=60)
                                                                                   GGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAAC            >  SRR4302540.2931847/1‑94 (MQ=60)
                                                                                   GGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAAC            >  SRR4302540.7042488/1‑94 (MQ=60)
                                                                                   GGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAAC            >  SRR4302540.8887920/1‑94 (MQ=60)
                                                                                     TTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAACCA          <  SRR4302540.9210367/94‑1 (MQ=60)
                                                                                     TTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAACCA          <  SRR4302540.2913307/94‑1 (MQ=60)
                                                                                      TGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAACCAC         <  SRR4302540.3185187/94‑1 (MQ=60)
                                                                                       GATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAACCACG        <  SRR4302540.8483506/94‑1 (MQ=60)
                                                                                        ATAGCAGCCCCACCAGCAGAAAGGTGACTGGGGTTATAACCATCAGACAGAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAACCACGC       <  SRR4302540.8573335/94‑1 (MQ=60)
                                                                                         TAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAAACATCAGACATAGCCATGGTGTGAAGAAATTTTTGATTGCCGACGGTACCCACGCT      >  SRR4302540.9817884/1‑94 (MQ=60)
                                                                                          AGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAACCACGCAT     >  SRR4302540.10055087/1‑94 (MQ=60)
                                                                                          AGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAACCACGCAT     <  SRR4302540.4357718/94‑1 (MQ=60)
                                                                                          AGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAACCACGCAT     >  SRR4302540.5334117/1‑94 (MQ=60)
                                                                                          AGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAACCACGCAT     <  SRR4302540.6884261/94‑1 (MQ=60)
                                                                                          AGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAACCACGCAT     <  SRR4302540.7171200/94‑1 (MQ=60)
                                                                                           GCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAACCACGCATT    <  SRR4302540.8915655/94‑1 (MQ=60)
                                                                                           GCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAACCACGCATT    <  SRR4302540.7250120/94‑1 (MQ=60)
                                                                                             AGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCACTGGTGTGAAGAAATTTTTGATTGCCGACGGTAACCACGCATTAA  >  SRR4302540.111867/1‑94 (MQ=60)
                                                                                             AGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAACCACGCATTAA  <  SRR4302540.3177769/94‑1 (MQ=60)
                                                                                             AGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAACCACGCATTAA  <  SRR4302540.6846314/94‑1 (MQ=60)
                                                                                             AGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAACCACGCATTAA  >  SRR4302540.111866/1‑94 (MQ=60)
                                                                                             AGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAACCACGCATTAA  >  SRR4302540.9831366/1‑94 (MQ=60)
                                                                                             |                                                                                             
AAGCCGCCCATTACCGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCGGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAACCACGCATTAA  >  NC_000913/3902339‑3902525

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑