Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,902,432 G→A P265L (CCG→CTG)  bglF ← PTS system beta‑glucosides‑specific enzyme IIABC component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,902,4320GA100.0% 877.4 / NA 235P265L (CCG→CTG) bglFPTS system beta‑glucosides‑specific enzyme IIABC component
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (119/116);  total (119/116)

Only 100 of 238 total aligned reads displayed.

CCGCCCATTACCGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCGGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAACCACGCA  >  NC_000913/3902342‑3902521
                                                                                          |                                                                                         
 cgccCATTACCGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGcc                                                                                        >  1:5779312/1‑93 (MQ=255)
 cgccCATTACCGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGcc                                                                                        <  1:3877260/93‑1 (MQ=255)
  gccCATTACCGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGccc                                                                                       >  1:5964112/1‑93 (MQ=255)
  gccCATTACCGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGccc                                                                                       >  1:2935942/1‑93 (MQ=255)
  gccCATTACCGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGccc                                                                                       >  2:5479924/1‑93 (MQ=255)
  gccCATTACCGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGccc                                                                                       >  2:3935972/1‑93 (MQ=255)
   cccATTACCGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGcccc                                                                                      >  1:6191604/1‑93 (MQ=255)
    ccATTACCGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCcca                                                                                     >  2:1200023/1‑93 (MQ=255)
    ccATTACCGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCcca                                                                                     >  2:1096883/1‑93 (MQ=255)
    ccATTACCGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCcca                                                                                     <  1:150098/93‑1 (MQ=255)
     cATTACCGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCccac                                                                                    >  1:263583/1‑93 (MQ=255)
     cATTACCGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCccac                                                                                    >  2:5045840/1‑93 (MQ=255)
      aTTACCGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCccacc                                                                                   >  2:171477/1‑93 (MQ=255)
      aTTACCGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCccacc                                                                                   >  2:2896610/1‑93 (MQ=255)
       ttACCGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCccacca                                                                                  <  2:2168477/93‑1 (MQ=255)
         aCCGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACcagc                                                                                >  1:1958854/1‑93 (MQ=255)
         aCCGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACcagc                                                                                <  2:1687337/93‑1 (MQ=255)
          ccGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACcagca                                                                               <  2:3584658/93‑1 (MQ=255)
            gcgcCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGa                                                                             >  1:4411706/1‑93 (MQ=255)
              gcCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGaaa                                                                           <  2:6245463/93‑1 (MQ=255)
                ccGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGACAgg                                                                         >  1:4275445/1‑93 (MQ=255)
                ccGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAgg                                                                         <  1:918604/93‑1 (MQ=255)
                 cGCAAATGCAGGAACCGCCTGATAAAGGCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGt                                                                        <  2:5744372/93‑1 (MQ=255)
                 cGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGt                                                                        >  2:17450/1‑93 (MQ=255)
                  gCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTg                                                                       >  2:1840476/1‑93 (MQ=255)
                  gCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTg                                                                       <  1:2778148/93‑1 (MQ=255)
                   cAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGa                                                                      <  1:2807572/93‑1 (MQ=255)
                   cAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGa                                                                      >  1:2218994/1‑93 (MQ=255)
                   cAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGa                                                                      <  1:2164304/93‑1 (MQ=255)
                    aaaTGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTGTCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGAc                                                                     <  1:3217726/93‑1 (MQ=255)
                     aaTGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACg                                                                    >  2:4442101/1‑93 (MQ=255)
                     aaTGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACg                                                                    <  1:5418565/93‑1 (MQ=255)
                        gCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGgt                                                                 >  2:3971927/1‑93 (MQ=255)
                          aGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGgtgt                                                               <  2:1738373/93‑1 (MQ=255)
                          aGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGgtgt                                                               >  2:2972372/1‑93 (MQ=255)
                          aGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGgtgt                                                               >  2:805560/1‑93 (MQ=255)
                            gAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGa                                                             >  2:1549807/1‑93 (MQ=255)
                            gAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGa                                                             <  2:5942325/93‑1 (MQ=255)
                             aaCCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGAt                                                            <  1:1334033/93‑1 (MQ=255)
                             aaCCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGAt                                                            <  1:584126/93‑1 (MQ=255)
                              aCCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATa                                                           <  1:6171246/93‑1 (MQ=255)
                              aCCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATa                                                           >  1:4151266/1‑93 (MQ=255)
                               ccGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATaa                                                          <  2:534640/93‑1 (MQ=255)
                                 gCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAAcc                                                        <  1:5190952/93‑1 (MQ=255)
                                  ccTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCa                                                       <  1:4611753/93‑1 (MQ=255)
                                   cTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCAt                                                      >  1:3366064/1‑93 (MQ=255)
                                   cTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCAt                                                      <  1:2571325/93‑1 (MQ=255)
                                    tGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCAta                                                     >  1:721075/1‑92 (MQ=255)
                                    tGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATc                                                     <  1:4308347/93‑1 (MQ=255)
                                    tGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATc                                                     <  2:4158373/93‑1 (MQ=255)
                                     gATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCa                                                    >  2:4340570/1‑93 (MQ=255)
                                       tAAATCCAGAGATAGCCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGa                                                  <  1:5711715/93‑1 (MQ=255)
                                         aaGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACa                                                <  1:1281921/93‑1 (MQ=255)
                                         aaGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACa                                                <  1:4537131/93‑1 (MQ=255)
                                          aGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACAt                                               <  2:6046152/93‑1 (MQ=255)
                                           gCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATa                                              >  1:2861008/1‑93 (MQ=255)
                                            ccAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAg                                             <  2:3277592/93‑1 (MQ=255)
                                             cAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGc                                            >  2:225767/1‑93 (MQ=255)
                                              agagATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCa                                           >  1:2405017/1‑93 (MQ=255)
                                              agagATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCa                                           >  2:4727782/1‑93 (MQ=255)
                                               gagaTAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCaa                                          <  1:3597514/93‑1 (MQ=255)
                                                agaTAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAAt                                         <  1:16551/93‑1 (MQ=255)
                                                 gaTAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATg                                        <  2:3517005/93‑1 (MQ=255)
                                                 gaTAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATg                                        >  2:1589144/1‑93 (MQ=255)
                                                 gaTAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATg                                        >  1:4769235/1‑93 (MQ=255)
                                                   tAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGgt                                      >  2:754685/1‑93 (MQ=255)
                                                    aaCCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGgtg                                     <  1:5353728/93‑1 (MQ=255)
                                                     aCCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGgtgt                                    <  2:3828033/93‑1 (MQ=255)
                                                           ggcAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTgaagaa                              >  2:1404462/1‑93 (MQ=255)
                                                           ggcAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTgaagaa                              >  1:4965048/1‑93 (MQ=255)
                                                             cAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAAt                            <  1:4703894/93‑1 (MQ=255)
                                                             cAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAAt                            <  1:5797173/93‑1 (MQ=255)
                                                              aaTCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAAtt                           >  1:199495/1‑93 (MQ=255)
                                                               aTCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAAttt                          >  2:2200134/1‑93 (MQ=255)
                                                               aTCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAAttt                          >  1:4017391/1‑93 (MQ=255)
                                                               aTCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAAttt                          >  2:2625768/1‑93 (MQ=255)
                                                                 cAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAAttttt                        <  2:3564199/93‑1 (MQ=255)
                                                                    ttCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGAt                     <  2:4841460/93‑1 (MQ=255)
                                                                      cGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTg                   >  1:1101176/1‑93 (MQ=255)
                                                                       gCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGc                  <  2:3387236/93‑1 (MQ=255)
                                                                       gCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGc                  >  2:249369/1‑93 (MQ=255)
                                                                        cTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGcc                 <  2:2819437/93‑1 (MQ=255)
                                                                          tATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGa               <  1:1525093/93‑1 (MQ=255)
                                                                          tATCCAGGGTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGa               <  1:5660687/93‑1 (MQ=255)
                                                                           aTCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGGGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGAc              <  1:3014675/93‑1 (MQ=255)
                                                                            tCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACg             <  1:5052602/93‑1 (MQ=255)
                                                                            tCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACg             <  2:1354031/93‑1 (MQ=255)
                                                                             ccAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACgg            <  1:3305344/93‑1 (MQ=255)
                                                                              cAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGt           <  2:4662776/93‑1 (MQ=255)
                                                                               aGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTa          >  1:2446556/1‑93 (MQ=255)
                                                                                ggTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTaa         >  2:4862095/1‑93 (MQ=255)
                                                                                ggTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTaa         >  2:63930/1‑93 (MQ=255)
                                                                                  ttGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAAca       >  2:4629272/1‑92 (MQ=255)
                                                                                   tGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAACCa      <  2:1061368/93‑1 (MQ=255)
                                                                                   tGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAACCa      <  1:6064894/93‑1 (MQ=255)
                                                                                     aTAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAACCACg    >  2:5847687/1‑93 (MQ=255)
                                                                                      tAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAACCACGc   <  1:3472097/93‑1 (MQ=255)
                                                                                      tAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAACCACGc   <  2:2708256/93‑1 (MQ=255)
                                                                                      tAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAACCACGc   >  1:5487328/1‑93 (MQ=255)
                                                                                       agcagcCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAACCACGCa  <  2:5216596/93‑1 (MQ=255)
                                                                                          |                                                                                         
CCGCCCATTACCGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCGGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAACCACGCA  >  NC_000913/3902342‑3902521

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AAGCCGCCCATTACCGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCGGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAACCACGCATTAA  >  NC_000913/3902339‑3902525
                                                                                             |                                                                                             
AAGCCGCCCATTACCGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCA                                                                                               <  SRR4302533.2569021/94‑1 (MQ=60)
 AACCGCCCATTACCGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAG                                                                                              >  SRR4302533.5978115/1‑94 (MQ=60)
  GCCGCCCATTACCGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGC                                                                                             <  SRR4302533.3198633/94‑1 (MQ=60)
   CCGCCCATTACCGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCC                                                                                            >  SRR4302533.5814217/1‑94 (MQ=60)
   CCGCCCATTACCGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCC                                                                                            >  SRR4302533.5316220/1‑94 (MQ=60)
    CGCCCATTACCGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCC                                                                                           <  SRR4302533.3900923/94‑1 (MQ=60)
    CGCCCATTACCGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCC                                                                                           >  SRR4302533.2953892/1‑94 (MQ=60)
    CGCCCATTACCGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCC                                                                                           >  SRR4302533.3686437/1‑94 (MQ=60)
    CGCCCATTACCGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCC                                                                                           >  SRR4302533.6000197/1‑94 (MQ=60)
     GCCCATTACCGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCC                                                                                          >  SRR4302533.6228762/1‑94 (MQ=60)
       CCATTACCGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCAC                                                                                        <  SRR4302533.151062/94‑1 (MQ=60)
       CCATTACCGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCAC                                                                                        <  SRR4302533.503799/94‑1 (MQ=60)
       CCATTACCGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCAC                                                                                        >  SRR4302533.3044624/1‑94 (MQ=60)
       CCATTACCGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCAC                                                                                        >  SRR4302533.5444691/1‑94 (MQ=60)
       CCATTACCGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCAC                                                                                        >  SRR4302533.265181/1‑94 (MQ=60)
       CCATTACCGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCAC                                                                                        <  SRR4302533.2221764/94‑1 (MQ=60)
        CATTACCGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACC                                                                                       >  SRR4302533.4325936/1‑94 (MQ=60)
         ATTACCGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCA                                                                                      >  SRR4302533.5370857/1‑94 (MQ=60)
          TTACCGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAG                                                                                     <  SRR4302533.3286961/94‑1 (MQ=60)
           TACCGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGC                                                                                    >  SRR4302533.6216502/1‑94 (MQ=60)
           TACCGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGC                                                                                    >  SRR4302533.1970810/1‑94 (MQ=60)
             CCGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGCTGATAGCAGCCCCACCAGCAG                                                                                  >  SRR4302533.152683/1‑94 (MQ=60)
              CGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGA                                                                                 >  SRR4302533.4438350/1‑94 (MQ=60)
                 GCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAG                                                                              >  SRR4302533.3856254/1‑94 (MQ=60)
                  CCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGACAGG                                                                             >  SRR4302533.4301163/1‑94 (MQ=60)
                  CCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGG                                                                             <  SRR4302533.3006120/94‑1 (MQ=60)
                   CCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGT                                                                            <  SRR4302533.2801446/94‑1 (MQ=60)
                   CCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGT                                                                            >  SRR4302533.584531/1‑94 (MQ=60)
                   CCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGT                                                                            <  SRR4302533.924002/94‑1 (MQ=60)
                    CGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTG                                                                           <  SRR4302533.2761919/94‑1 (MQ=60)
                     GCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGA                                                                          >  SRR4302533.2232693/1‑94 (MQ=60)
                     GCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGA                                                                          <  SRR4302533.2795213/94‑1 (MQ=60)
                      CAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGAC                                                                         <  SRR4302533.2177619/94‑1 (MQ=60)
                      CAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGAC                                                                         <  SRR4302533.2824850/94‑1 (MQ=60)
                       AAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTGTCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACG                                                                        <  SRR4302533.3237567/94‑1 (MQ=60)
                       AAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACG                                                                        <  SRR4302533.3554381/94‑1 (MQ=60)
                       AAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACG                                                                        <  SRR4302533.6176089/94‑1 (MQ=60)
                        AATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGG                                                                       <  SRR4302533.5451159/94‑1 (MQ=60)
                          TGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGT                                                                     <  SRR4302533.4748681/94‑1 (MQ=60)
                          AGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGT                                                                     <  SRR4302533.5189356/94‑1 (MQ=60)
                           GCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTG                                                                    >  SRR4302533.594744/1‑94 (MQ=60)
                             AGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTG                                                                  >  SRR4302533.2440806/1‑94 (MQ=60)
                              GGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGA                                                                 <  SRR4302533.6211978/94‑1 (MQ=60)
                               GAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGAT                                                                <  SRR4302533.4266128/94‑1 (MQ=60)
                                AACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATA                                                               <  SRR4302533.1342039/94‑1 (MQ=60)
                                AACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATA                                                               <  SRR4302533.587571/94‑1 (MQ=60)
                                AACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATA                                                               >  SRR4302533.4176421/1‑94 (MQ=60)
                                 ACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAA                                                              <  SRR4302533.6208332/94‑1 (MQ=60)
                                  CCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAAC                                                             >  SRR4302533.5567996/1‑94 (MQ=60)
                                   CGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACC                                                            >  SRR4302533.582857/1‑94 (MQ=60)
                                    GCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCA                                                           <  SRR4302533.5222269/94‑1 (MQ=60)
                                     CCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCAT                                                          >  SRR4302533.3386855/1‑94 (MQ=60)
                                     CCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCAT                                                          <  SRR4302533.4639695/94‑1 (MQ=60)
                                     CCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCAT                                                          >  SRR4302533.2689706/1‑94 (MQ=60)
                                      CTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATC                                                         <  SRR4302533.1727228/94‑1 (MQ=60)
                                      CTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATC                                                         <  SRR4302533.2587149/94‑1 (MQ=60)
                                      CTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATA                                                         >  SRR4302533.725329/1‑94 (MQ=60)
                                       TGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCA                                                        <  SRR4302533.4334279/94‑1 (MQ=60)
                                       TGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCA                                                        >  SRR4302533.2576112/1‑94 (MQ=60)
                                        GATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAG                                                       <  SRR4302533.4430095/94‑1 (MQ=60)
                                        GATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAG                                                       <  SRR4302533.470150/94‑1 (MQ=60)
                                         ATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGA                                                      >  SRR4302533.4822998/1‑94 (MQ=60)
                                         ATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGA                                                      >  SRR4302533.2453202/1‑94 (MQ=60)
                                          TAAATCCAGAGATAGCCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGAC                                                     <  SRR4302533.5746216/94‑1 (MQ=60)
                                            AAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACAT                                                   <  SRR4302533.4564601/94‑1 (MQ=60)
                                            AAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACAT                                                   <  SRR4302533.1289636/94‑1 (MQ=60)
                                             AGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATA                                                  >  SRR4302533.2878608/1‑94 (MQ=60)
                                             AGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATA                                                  <  SRR4302533.2832263/94‑1 (MQ=60)
                                              GCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAG                                                 <  SRR4302533.5706626/94‑1 (MQ=60)
                                              GCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAG                                                 >  SRR4302533.1992608/1‑94 (MQ=60)
                                               CCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGC                                                >  SRR4302533.5035752/1‑94 (MQ=60)
                                                CAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCA                                               >  SRR4302533.2419869/1‑94 (MQ=60)
                                                  GAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAAT                                             <  SRR4302533.1597823/94‑1 (MQ=60)
                                                  GAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAAT                                             <  SRR4302533.3619649/94‑1 (MQ=60)
                                                   AGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATG                                            >  SRR4302533.4798142/1‑94 (MQ=60)
                                                   AGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATG                                            <  SRR4302533.16666/94‑1 (MQ=60)
                                                   AGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATG                                            >  SRR4302533.1437201/1‑94 (MQ=60)
                                                     ATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGCGTGATAACCATCAGACATAGCAATGGG                                          >  SRR4302533.1311354/1‑94 (MQ=60)
                                                       AACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGT                                        <  SRR4302533.5385859/94‑1 (MQ=60)
                                                        ACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTG                                       <  SRR4302533.6273128/94‑1 (MQ=60)
                                                         CCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGA                                      <  SRR4302533.4031846/94‑1 (MQ=60)
                                                          CGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAA                                     <  SRR4302533.2617423/94‑1 (MQ=60)
                                                          CGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAA                                     <  SRR4302533.813288/94‑1 (MQ=60)
                                                          CGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAA                                     <  SRR4302533.5762905/94‑1 (MQ=60)
                                                           GGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAG                                    >  SRR4302533.1479895/1‑94 (MQ=60)
                                                             CGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAA                                  >  SRR4302533.4995144/1‑94 (MQ=60)
                                                             CGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAA                                  >  SRR4302533.45838/1‑94 (MQ=60)
                                                                CAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATT                               <  SRR4302533.4732408/94‑1 (MQ=60)
                                                                CAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATT                               >  SRR4302533.5674371/1‑94 (MQ=60)
                                                                CAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATT                               <  SRR4302533.5832197/94‑1 (MQ=60)
                                                                CAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATT                               >  SRR4302533.200756/1‑94 (MQ=60)
                                                                 AATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTT                              >  SRR4302533.4041851/1‑94 (MQ=60)
                                                                  ATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGGGATAAGCATCAGACATAGCAATGGTGTGAAGAAATTTT                             >  SRR4302533.3379452/1‑94 (MQ=60)
                                                                   TCAGTTCGCTTATCCAGGTTGATCGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTT                            >  SRR4302533.2069461/1‑94 (MQ=60)
                                                                     AGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGA                          >  SRR4302533.416014/1‑94 (MQ=60)
                                                                     AGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGA                          >  SRR4302533.2848444/1‑94 (MQ=60)
                                                                      GTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGAT                         >  SRR4302533.1929551/1‑94 (MQ=60)
                                                                      GTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGAT                         >  SRR4302533.469940/1‑94 (MQ=60)
                                                                        TCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTG                       >  SRR4302533.1107703/1‑94 (MQ=60)
                                                                         CGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGC                      >  SRR4302533.5888667/1‑94 (MQ=60)
                                                                           CTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCG                    >  SRR4302533.6018627/1‑94 (MQ=60)
                                                                           CTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCG                    <  SRR4302533.5815049/94‑1 (MQ=60)
                                                                           CTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCG                    >  SRR4302533.2950458/1‑94 (MQ=60)
                                                                            TTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGA                   <  SRR4302533.5462724/94‑1 (MQ=60)
                                                                            TTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGA                   >  SRR4302533.3441879/1‑94 (MQ=60)
                                                                             TATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGAC                  <  SRR4302533.1534275/94‑1 (MQ=60)
                                                                             TATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGAC                  >  SRR4302533.828850/1‑94 (MQ=60)
                                                                             TATCCAGGGTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGAC                  <  SRR4302533.5694889/94‑1 (MQ=60)
                                                                              ATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACG                 <  SRR4302533.4237390/94‑1 (MQ=60)
                                                                              ATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACG                 >  SRR4302533.3237237/1‑94 (MQ=60)
                                                                              ATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGGGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACG                 <  SRR4302533.3033112/94‑1 (MQ=60)
                                                                               TCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGG                <  SRR4302533.5083151/94‑1 (MQ=60)
                                                                                CCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGT               <  SRR4302533.3325754/94‑1 (MQ=60)
                                                                                CCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGT               <  SRR4302533.2052861/94‑1 (MQ=60)
                                                                                 CAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTA              <  SRR4302533.1186438/94‑1 (MQ=60)
                                                                                 CAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTA              <  SRR4302533.4517562/94‑1 (MQ=60)
                                                                                 CAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTA              >  SRR4302533.2461655/1‑94 (MQ=60)
                                                                                  AGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAA             >  SRR4302533.2277051/1‑94 (MQ=60)
                                                                                   GGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAAC            <  SRR4302533.347886/94‑1 (MQ=60)
                                                                                    GTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAACC           <  SRR4302533.4417796/94‑1 (MQ=60)
                                                                                      TGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAACCAC         <  SRR4302533.6101604/94‑1 (MQ=60)
                                                                                        ATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAACCACGC       >  SRR4302533.5520409/1‑94 (MQ=60)
                                                                                         TAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAACCACGCA      <  SRR4302533.3493523/94‑1 (MQ=60)
                                                                                           GCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAACCACGCATT    >  SRR4302533.3241442/1‑94 (MQ=60)
                                                                                           GCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAACCACGCATT    <  SRR4302533.137425/94‑1 (MQ=60)
                                                                                            CAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAACCACGCATTA   >  SRR4302533.2430062/1‑94 (MQ=60)
                                                                                             AGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAACCACGCATTAA  >  SRR4302533.5071737/1‑94 (MQ=60)
                                                                                             AGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAACCACGCATTAA  <  SRR4302533.4541047/94‑1 (MQ=60)
                                                                                             AGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAACCACGCATTAA  <  SRR4302533.987567/94‑1 (MQ=60)
                                                                                             |                                                                                             
AAGCCGCCCATTACCGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCGGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAACCACGCATTAA  >  NC_000913/3902339‑3902525

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑