Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,917,524	G→A	R170C (CGC→TGC)	atpH ←	membrane‑bound ATP synthase, F1 sector, delta‑subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,917,524	0	G	A	99.6%	990.3 / ‑5.6	273	R170C (CGC→TGC)	atpH	membrane‑bound ATP synthase, F1 sector, delta‑subunit
Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (131/141); total (131/142)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01

Only 100 of 278 total aligned reads displayed.

GAGCAATGCGCTGCTTGATCAGTTCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCGCTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCGATTTTGCAAT  >  NC_000913/3917434‑3917613

gAGCAATGCGCTGCTTGATCAGTTCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACt                                                                                         >  1:3181174/1‑93 (MQ=255)

 aGCAATGCGCTGCTTGATCAGTTCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTc                                                                                        >  1:1043443/1‑93 (MQ=255)

 aGCAATGCGCTGCTTGATCAGTTCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTc                                                                                        <  1:5074672/93‑1 (MQ=255)

    aaTGCGCTGCTTGATCAGTTCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAg                                                                                     >  2:4303826/1‑93 (MQ=255)

     aTGCGCTGCTTGATCAGTTCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAga                                                                                    <  2:4296862/93‑1 (MQ=255)

        cgcTGCTTGATCAGTTCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAgacga                                                                                 <  2:1071610/93‑1 (MQ=255)

         gctgctTGATCAGTTCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAgacgac                                                                                <  2:3014535/93‑1 (MQ=255)

          ctgctTGATCAGTTCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGAcc                                                                               <  2:3496125/93‑1 (MQ=255)

          ctgctTGATCAGTTCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGAcc                                                                               <  1:4384207/93‑1 (MQ=255)

            gctTGATCAGTTCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACcgc                                                                             >  2:5376391/1‑93 (MQ=255)

            gctTGATCAGTTCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACcgc                                                                             >  1:24348/1‑93 (MQ=255)

              ttGATCAGTTCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGt                                                                           >  2:1272130/1‑93 (MQ=255)

               tGATCAGTTCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTa                                                                          <  1:2563796/93‑1 (MQ=255)

                 aTCAGTTCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTAcc                                                                        >  2:3281590/1‑92 (MQ=255)

                 aTCAGTTCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACg                                                                        <  2:4837912/93‑1 (MQ=255)

                 aTCAGTTCGCTGATATCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACg                                                                        >  2:3848821/1‑93 (MQ=255)

                  tCAGTTCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGc                                                                       <  1:2122522/93‑1 (MQ=255)

                  tCAGTTCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGc                                                                       <  2:424738/93‑1 (MQ=255)

                  tCAGTTCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGc                                                                       >  2:2160209/1‑93 (MQ=255)

                  tCAGTTCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGc                                                                       >  1:6067748/1‑93 (MQ=255)

                   cAGTTCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCGAGGCACTCAAGACGACCGCGTACGCt                                                                      >  1:2573809/1‑93 (MQ=255)

                   cAGTTCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCt                                                                      <  2:2147326/93‑1 (MQ=255)

                   cAGTTCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCt                                                                      <  2:372239/93‑1 (MQ=255)

                    aGTTCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGTCTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTg                                                                     >  1:3610783/1‑93 (MQ=255)

                     gTTCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGc                                                                    >  1:3984645/1‑93 (MQ=255)

                      ttCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGcc                                                                   >  2:2823636/1‑93 (MQ=255)

                      ttCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGcc                                                                   >  2:1383349/1‑93 (MQ=255)

                      ttCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGcc                                                                   <  1:1529323/93‑1 (MQ=255)

                       tCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCa                                                                  <  2:6111585/93‑1 (MQ=255)

                       tCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCa                                                                  <  2:4453860/93‑1 (MQ=255)

                       tCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCa                                                                  <  2:5515546/93‑1 (MQ=255)

                         gCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGCCCCCTTAAAATAGCAAACCGCCTGCAAGGAAATCAAGACGCCGCCGTACGCGGCCAta                                                                >  2:2948806/1‑92 (MQ=255)

                         ccTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATc                                                                <  1:1541822/92‑1 (MQ=255)

                          cTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCa                                                               <  2:3218387/93‑1 (MQ=255)

                          cTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCa                                                               >  2:1489625/1‑93 (MQ=255)

                           tGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCaa                                                              >  2:850131/1‑93 (MQ=255)

                           tGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCaa                                                              >  2:4084574/1‑93 (MQ=255)

                           tGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCaa                                                              >  2:1317457/1‑93 (MQ=255)

                           tGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCaa                                                              <  1:3914382/93‑1 (MQ=255)

                           tGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCaa                                                              <  1:3427774/93‑1 (MQ=255)

                            gATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAAt                                                             <  2:2764555/93‑1 (MQ=255)

                            gATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAAt                                                             >  2:5056599/1‑93 (MQ=255)

                              tttCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGa                                                           >  1:1869049/1‑93 (MQ=255)

                              tttCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGa                                                           <  1:1041672/93‑1 (MQ=255)

                                   gTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCata                                                      >  1:5939924/1‑93 (MQ=255)

                                   gTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCata                                                      >  1:9634/1‑93 (MQ=255)

                                    tGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCatat                                                     <  2:1960215/93‑1 (MQ=255)

                                      gAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCa                                                   <  2:1631428/93‑1 (MQ=255)

                                          tCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCg                                               <  2:6201612/93‑1 (MQ=255)

                                          tCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCg                                               >  2:5841118/1‑93 (MQ=255)

                                           cAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGc                                              <  2:173240/93‑1 (MQ=255)

                                             gTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTc                                            >  2:382974/1‑93 (MQ=255)

                                             gTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTc                                            <  1:2067438/93‑1 (MQ=255)

                                                gCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGga                                         >  2:5136352/1‑93 (MQ=255)

                                                gCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGga                                         <  1:533274/93‑1 (MQ=255)

                                                gCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGga                                         >  1:5882932/1‑93 (MQ=255)

                                                gCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGga                                         <  1:274351/93‑1 (MQ=255)

                                                 cATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGgat                                        <  2:2114704/93‑1 (MQ=255)

                                                 cATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGgat                                        <  2:5600642/93‑1 (MQ=255)

                                                   tGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGgatga                                      <  1:328624/93‑1 (MQ=255)

                                                    gCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGgatgat                                     <  2:1897689/93‑1 (MQ=255)

                                                     cgccAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCGCTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATa                                    <  1:6281942/91‑1 (MQ=255)

                                                      tCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATaa                                   >  1:2375367/1‑93 (MQ=255)

                                                       ccAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAAc                                  <  2:5276580/93‑1 (MQ=255)

                                                       ccAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAAc                                  >  1:3441081/1‑93 (MQ=255)

                                                         aGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGc                                <  1:3088229/93‑1 (MQ=255)

                                                         aGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGc                                >  2:3286606/1‑93 (MQ=255)

                                                          gTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGcc                               >  1:5834497/1‑93 (MQ=255)

                                                           tCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCt                              >  1:4054959/1‑93 (MQ=255)

                                                           tCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCt                              <  2:6040256/93‑1 (MQ=255)

                                                           tCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCt                              >  1:3940524/1‑93 (MQ=255)

                                                            ccccTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTg                             <  1:1783013/93‑1 (MQ=255)

                                                             cccTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGc                            >  2:4754829/1‑93 (MQ=255)

                                                               cTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCa                          >  2:6237890/1‑93 (MQ=255)

                                                               cTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATAAATGACCATATAACCAGCTCGGATGATAACGCCTGCCa                          >  1:1111870/1‑93 (MQ=255)

                                                                ttAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCAt                         <  1:1658051/93‑1 (MQ=255)

                                                                ttAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCAt                         <  1:1296494/93‑1 (MQ=255)

                                                                ttAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCAt                         >  1:4227970/1‑93 (MQ=255)

                                                                 tAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCAtt                        <  2:4607636/93‑1 (MQ=255)

                                                                 tAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCAtt                        <  1:5485659/93‑1 (MQ=255)

                                                                 tAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCAtt                        <  1:480219/93‑1 (MQ=255)

                                                                 tAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCAtt                        >  1:4863192/1‑93 (MQ=255)

                                                                    gACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACa                     <  2:5740972/93‑1 (MQ=255)

                                                                     aCTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAg                    <  1:2669190/93‑1 (MQ=255)

                                                                      cTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGa                   <  1:3238527/93‑1 (MQ=255)

                                                                      cTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGa                   <  2:1349787/93‑1 (MQ=255)

                                                                      cTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGa                   <  2:5435682/93‑1 (MQ=255)

                                                                       tGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGAc                  >  1:4394193/1‑93 (MQ=255)

                                                                       cgCAAGACGTCGGCAAGCCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGAc                  <  2:93057/92‑1 (MQ=255)

                                                                          aaGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTa               <  2:2037552/93‑1 (MQ=255)

                                                                          aaGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTa               <  1:495109/93‑1 (MQ=255)

                                                                             aCGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCg            <  1:5721155/93‑1 (MQ=255)

                                                                              cGTCTGCAAGGCACTCAAGCCGACCGCGTACGCTGCCTTCAATGCCCTTATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCGa           <  1:6300943/93‑1 (MQ=255)

                                                                               gTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCGAt          >  2:4034954/1‑93 (MQ=255)

                                                                                tCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCGAtt         <  2:4727008/93‑1 (MQ=255)

                                                                                tCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCGAtt         >  1:2854611/1‑93 (MQ=255)

                                                                                tCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCGAtt         >  1:3766329/1‑93 (MQ=255)

                                                                                tCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCGAtt         >  2:2340230/1‑93 (MQ=255)

                                                                                tCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCGAtt         >  1:5790550/1‑93 (MQ=255)

                                                                                   gCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCGATTTTg      >  2:5692501/1‑93 (MQ=255)

GAGCAATGCGCTGCTTGATCAGTTCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCGCTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCGATTTTGCAAT  >  NC_000913/3917434‑3917613

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

ACTGAGCAATGCGCTGCTTGATCAGTTCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCGCTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCGATTTTGCAATTCAG  >  NC_000913/3917431‑3917617

ACTGAGCAATGCGCTGCTTGATCAGTTCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCA                                                                                               <  SRR4302533.1135855/94‑1 (MQ=60)

 CTGAGCAATGCGCTGCTTGATCAGTTCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCAC                                                                                              >  SRR4302533.5255692/1‑94 (MQ=60)

  TGAGCAATGCGCTGCTTGATCAGTTCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACT                                                                                             >  SRR4302533.3200745/1‑94 (MQ=60)

  TGAGCAATGCGCTGCTTGATCAGTTCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACT                                                                                             <  SRR4302533.4280606/94‑1 (MQ=60)

   GAGCAATGCGCTGCTTGATCAGTTCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTC                                                                                            >  SRR4302533.1049577/1‑94 (MQ=60)

   GAGCAATGCGCTGCTTGATCAGTTCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTC                                                                                            >  SRR4302533.3471837/1‑94 (MQ=60)

    AGCAATGCGCTGCTTGATCAGTTCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCA                                                                                           <  SRR4302533.5105346/94‑1 (MQ=60)

      CAATGCGCTGCTTGATCAGTTCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAG                                                                                         <  SRR4302533.3939633/94‑1 (MQ=60)

      CAATGCGCTGCTTGATCAGTTCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAG                                                                                         >  SRR4302533.4168356/1‑94 (MQ=60)

            GCTGCTTGATCAGTTCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACC                                                                                   <  SRR4302533.1201689/94‑1 (MQ=60)

             CTGCTTGATCAGTTCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCG                                                                                  <  SRR4302533.4410675/94‑1 (MQ=60)

             CTGCTTGATCAGTTCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCG                                                                                  >  SRR4302533.2638070/1‑94 (MQ=60)

             CTGCTTGATCAGTTCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCG                                                                                  >  SRR4302533.3449019/1‑94 (MQ=60)

              TGCTTGATCAGTTCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGC                                                                                 >  SRR4302533.24509/1‑94 (MQ=60)

               TCTTGATCAGTTCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCG                                                                                <  SRR4302533.4857260/94‑1 (MQ=60)

                  TGATCAGTTCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTAC                                                                             <  SRR4302533.2579576/94‑1 (MQ=60)

                  TGATCAGTTCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTAC                                                                             <  SRR4302533.3271825/94‑1 (MQ=60)

                  TGATCAGTTCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTAC                                                                             <  SRR4302533.6331212/94‑1 (MQ=60)

                   GATCAGTTCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACG                                                                            >  SRR4302533.5948329/1‑94 (MQ=60)

                    ATCAGTTCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGC                                                                           >  SRR4302533.6104465/1‑94 (MQ=60)

                    ATCAGTTCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGC                                                                           >  SRR4302533.563726/1‑94 (MQ=60)

                     TCAGTTCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCGAGGCACTCAAGACGACCGCGTACGCT                                                                          >  SRR4302533.2589642/1‑94 (MQ=60)

                     TCAGTTCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCT                                                                          <  SRR4302533.499288/94‑1 (MQ=60)

                     TCAGTTCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCT                                                                          <  SRR4302533.2135604/94‑1 (MQ=60)

                     TCAGTTCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCT                                                                          >  SRR4302533.5802498/1‑94 (MQ=60)

                      CAGTTCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGTCTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTG                                                                         >  SRR4302533.3633006/1‑94 (MQ=60)

                       AGTTCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGC                                                                        >  SRR4302533.4008907/1‑94 (MQ=60)

                       AGTTCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGACACTCAAGACGACCGCGTACGCTGC                                                                        >  SRR4302533.3212709/1‑94 (MQ=60)

                         TTCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCA                                                                      >  SRR4302533.6000983/1‑94 (MQ=60)

                         TTCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCA                                                                      >  SRR4302533.3337093/1‑94 (MQ=60)

                         TTCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCA                                                                      >  SRR4302533.4330952/1‑94 (MQ=60)

                         TTCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCA                                                                      <  SRR4302533.3048698/94‑1 (MQ=60)

                         TTCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCA                                                                      <  SRR4302533.1538520/94‑1 (MQ=60)

                           CGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATC                                                                    <  SRR4302533.1769239/94‑1 (MQ=60)

                           CGCTGATTTCGGTGGAATTCAGGTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATC                                                                    <  SRR4302533.3872906/94‑1 (MQ=60)

                            CCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCA                                                                   <  SRR4302533.1551110/94‑1 (MQ=60)

                             CTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAA                                                                  <  SRR4302533.5510848/94‑1 (MQ=60)

                              TGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAAT                                                                 <  SRR4302533.3938293/94‑1 (MQ=60)

                              TGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAAT                                                                 <  SRR4302533.3448952/94‑1 (MQ=60)

                              TGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAAT                                                                 <  SRR4302533.2708514/94‑1 (MQ=60)

                              TGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAAT                                                                 >  SRR4302533.3466754/1‑94 (MQ=60)

                               GATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATG                                                                <  SRR4302533.4069130/94‑1 (MQ=60)

                               GATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATG                                                                >  SRR4302533.4415990/1‑94 (MQ=60)

                                ATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGA                                                               >  SRR4302533.2442096/1‑94 (MQ=60)

                                ATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGA                                                               >  SRR4302533.1880444/1‑94 (MQ=60)

                                 TTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGAC                                                              <  SRR4302533.1047796/94‑1 (MQ=60)

                                  TTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACC                                                             <  SRR4302533.2868740/94‑1 (MQ=60)

                                    CGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCAT                                                           <  SRR4302533.4261652/94‑1 (MQ=60)

                                     GGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATA                                                          >  SRR4302533.9716/1‑94 (MQ=60)

                                     GGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATA                                                          >  SRR4302533.5975838/1‑94 (MQ=60)

                                     GGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATA                                                          >  SRR4302533.3981531/1‑94 (MQ=60)

                                      GTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATAT                                                         >  SRR4302533.3867965/1‑94 (MQ=60)

                                      GTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATAT                                                         <  SRR4302533.662686/94‑1 (MQ=60)

                                       TGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATC                                                        <  SRR4302533.2269482/94‑1 (MQ=60)

                                        GGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCA                                                       <  SRR4302533.1200080/94‑1 (MQ=60)

                                         GAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCAC                                                      >  SRR4302533.619556/1‑94 (MQ=60)

                                         GAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCAC                                                      >  SRR4302533.619559/1‑94 (MQ=60)

                                          AATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACC                                                     <  SRR4302533.4845627/94‑1 (MQ=60)

                                           ATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCC                                                    <  SRR4302533.1398481/94‑1 (MQ=60)

                                            TTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCG                                                   <  SRR4302533.5914061/94‑1 (MQ=60)

                                            TTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCAGCAATGACCATATCACCCG                                                   <  SRR4302533.759697/94‑1 (MQ=60)

                                              CAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCT                                                 <  SRR4302533.1047949/94‑1 (MQ=60)

                                              CAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCT                                                 <  SRR4302533.4054362/94‑1 (MQ=60)

                                              CAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCT                                                 <  SRR4302533.1341401/94‑1 (MQ=60)

                                              CAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCT                                                 >  SRR4302533.3120759/1‑94 (MQ=60)

                                               AGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTC                                                >  SRR4302533.1622678/1‑94 (MQ=60)

                                                GTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCG                                               <  SRR4302533.5090654/94‑1 (MQ=60)

                                                GTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCG                                               <  SRR4302533.2080174/94‑1 (MQ=60)

                                                 TTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGG                                              >  SRR4302533.5393245/1‑94 (MQ=60)

                                                 TTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGG                                              >  SRR4302533.5911978/1‑94 (MQ=60)

                                                  TGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGA                                             >  SRR4302533.5918482/1‑94 (MQ=60)

                                                   GCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGAT                                            <  SRR4302533.276020/94‑1 (MQ=60)

                                                   GCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGAT                                            <  SRR4302533.536445/94‑1 (MQ=60)

                                                   GCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGAT                                            <  SRR4302533.2081313/94‑1 (MQ=60)

                                                   GCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGAT                                            <  SRR4302533.5972333/94‑1 (MQ=60)

                                                    CATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATG                                           <  SRR4302533.3756257/94‑1 (MQ=60)

                                                     ATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGA                                          >  SRR4302533.5725348/1‑94 (MQ=60)

                                                     ATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGA                                          <  SRR4302533.2173129/94‑1 (MQ=60)

                                                      TGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCTGATGAT                                         >  SRR4302533.2822833/1‑94 (MQ=60)

                                                      TGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGAT                                         <  SRR4302533.330629/94‑1 (MQ=60)

                                                       GCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATA                                        <  SRR4302533.3955747/94‑1 (MQ=60)

                                                       GCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATA                                        <  SRR4302533.2588388/94‑1 (MQ=60)

                                                        CTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAA                                       >  SRR4302533.1904709/1‑94 (MQ=60)

                                                        CTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAA                                       >  SRR4302533.3417350/1‑94 (MQ=60)

                                                        CTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAA                                       >  SRR4302533.2390066/1‑94 (MQ=60)

                                                        CGCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCGCTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAA                                       <  SRR4302533.6319434/94‑1 (MQ=60)

                                                         TCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAAC                                      <  SRR4302533.1738849/94‑1 (MQ=60)

                                                         TCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAAC                                      >  SRR4302533.3462329/1‑94 (MQ=60)

                                                         TCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAAC                                      <  SRR4302533.293036/94‑1 (MQ=60)

                                                         TCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAAC                                      <  SRR4302533.4973194/94‑1 (MQ=60)

                                                          CCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACG                                     <  SRR4302533.99534/94‑1 (MQ=60)

                                                            AGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCC                                   >  SRR4302533.5869756/1‑94 (MQ=60)

                                                            AGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCC                                   <  SRR4302533.3107214/94‑1 (MQ=60)

                                                             GTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCT                                  >  SRR4302533.3964570/1‑94 (MQ=60)

                                                             GTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCT                                  >  SRR4302533.4079647/1‑94 (MQ=60)

                                                             GTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCT                                  <  SRR4302533.3210696/94‑1 (MQ=60)

                                                              TCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTG                                 >  SRR4302533.1074250/1‑94 (MQ=60)

                                                              TCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTG                                 <  SRR4302533.5790613/94‑1 (MQ=60)

                                                              TCCCCTTAAGACTGCAAGACGTCTGCAAGCCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTG                                 <  SRR4302533.4148116/94‑1 (MQ=60)

                                                               CCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGC                                <  SRR4302533.1793860/94‑1 (MQ=60)

                                                                 CCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATAAATGACCATATAACCAGCTCGGATGATAACGCCTGCCA                              >  SRR4302533.1118469/1‑94 (MQ=60)

                                                                  CTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCAT                             >  SRR4302533.4253532/1‑94 (MQ=60)

                                                                  CTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCAT                             >  SRR4302533.366237/1‑94 (MQ=60)

                                                                   TTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATT                            >  SRR4302533.4892664/1‑94 (MQ=60)

                                                                   TTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATT                            <  SRR4302533.1668083/94‑1 (MQ=60)

                                                                   TTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATT                            <  SRR4302533.1304298/94‑1 (MQ=60)

                                                                    TAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTA                           <  SRR4302533.483090/94‑1 (MQ=60)

                                                                    TAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTA                           <  SRR4302533.5518723/94‑1 (MQ=60)

                                                                    TAAGACTCCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTA                           <  SRR4302533.4897336/94‑1 (MQ=60)

                                                                     AAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTAC                          >  SRR4302533.2678459/1‑94 (MQ=60)

                                                                       GACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAG                        <  SRR4302533.2000051/94‑1 (MQ=60)

                                                                       GACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAG                        <  SRR4302533.6033429/94‑1 (MQ=60)

                                                                        ACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGA                       >  SRR4302533.5258669/1‑94 (MQ=60)

                                                                        ACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGA                       <  SRR4302533.2685565/94‑1 (MQ=60)

                                                                         CTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGAC                      >  SRR4302533.4420724/1‑94 (MQ=60)

                                                                         CTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGAC                      <  SRR4302533.3258486/94‑1 (MQ=60)

                                                                            CAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTA                   >  SRR4302533.1544021/1‑94 (MQ=60)

                                                                            CAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTA                   >  SRR4302533.2515699/1‑94 (MQ=60)

                                                                             AAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTAT                  <  SRR4302533.2703527/94‑1 (MQ=60)

                                                                             AAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTAT                  <  SRR4302533.498059/94‑1 (MQ=60)

                                                                               GACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCG                >  SRR4302533.4823755/1‑94 (MQ=60)

                                                                                ACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCGA               >  SRR4302533.187880/1‑94 (MQ=60)

                                                                                ACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCGA               <  SRR4302533.5755698/94‑1 (MQ=60)

                                                                                 CGTCTGCAAGGCACTCAAGCCGACCGCGTACGCTGCCTTCAATGCCCTTATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCGAT              <  SRR4302533.6338578/94‑1 (MQ=60)

                                                                                  GTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCGATT             >  SRR4302533.3789419/1‑94 (MQ=60)

                                                                                  GTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCGATT             >  SRR4302533.5825534/1‑94 (MQ=60)

                                                                                  GTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCGATT             >  SRR4302533.2435138/1‑94 (MQ=60)

                                                                                  GTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCGATT             >  SRR4302533.465742/1‑94 (MQ=60)

                                                                                  GTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCGATT             >  SRR4302533.2872176/1‑94 (MQ=60)

                                                                                   TCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCGATTT            >  SRR4302533.1082938/1‑94 (MQ=60)

                                                                                    CTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCGATTTT           <  SRR4302533.157906/94‑1 (MQ=60)

                                                                                     TGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCGATTTTG          <  SRR4302533.5859909/94‑1 (MQ=60)

                                                                                     TGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCGATTTTG          >  SRR4302533.2474802/1‑94 (MQ=60)

                                                                                      GCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCGATTTTGC         <  SRR4302533.1869851/94‑1 (MQ=60)

                                                                                       CAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCGATTTTGCA        >  SRR4302533.3293988/1‑94 (MQ=60)

                                                                                           GCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCGATTTTGCAATTC    <  SRR4302533.5368777/94‑1 (MQ=60)

                                                                                            CACTCAGGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCGATTTTGCAATTCA   >  SRR4302533.1730671/1‑94 (MQ=60)

                                                                                             CCTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCCCCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCGATTTTGCAATTCAG  <  SRR4302533.4969693/94‑1 (MQ=60)

                                                                                             ACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCGATTTTGCAATTCAG  >  SRR4302533.5556500/1‑94 (MQ=60)

                                                                                             ACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCGATTTTGCAATTCAG  >  SRR4302533.5030250/1‑94 (MQ=60)

ACTGAGCAATGCGCTGCTTGATCAGTTCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCGCTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCGATTTTGCAATTCAG  >  NC_000913/3917431‑3917617

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 10 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`