Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,930,568 +G intergenic (+66/‑373) kup → / → rbsD low affinity potassium transport system/RbsD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,930,5671.G100.0% 661.9 / NA 207intergenic (+65/‑374)kup/rbsDlow affinity potassium transport system/RbsD
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (82/125);  total (82/125)

Only 100 of 233 total aligned reads displayed.

GCACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTT‑GCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTAT  >  NC_000913/3930475‑3930654
                                                                                             |                                                                                       
gcACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGtt                                                                                          <  1:1723065/93‑1 (MQ=255)
 cACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTT‑g                                                                                        <  1:1629231/93‑1 (MQ=255)
 cACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTT‑g                                                                                        >  2:4016502/1‑93 (MQ=255)
  aCCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTT‑gg                                                                                       <  1:1279558/93‑2 (MQ=255)
  aCCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTT‑gg                                                                                       <  1:374116/93‑2 (MQ=255)
  aCCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTT‑gg                                                                                       <  2:3333071/93‑2 (MQ=255)
   ccGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTT‑ggc                                                                                      <  2:1101045/93‑3 (MQ=255)
   ccGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTT‑ggc                                                                                      <  2:5710304/93‑3 (MQ=255)
     gAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTT‑ggcga                                                                                    >  2:1264829/1‑89 (MQ=255)
     gAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTT‑ggcga                                                                                    >  1:278361/1‑89 (MQ=255)
       aGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCgaga                                                                                   <  2:3011053/93‑1 (MQ=255)
         aCGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGaaa                                                                                 <  2:914975/93‑1 (MQ=255)
         aCGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGaaa                                                                                 <  1:5412495/93‑1 (MQ=255)
          cGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAAc                                                                                >  1:5470244/1‑93 (MQ=255)
           gCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACg                                                                               <  2:2372145/93‑1 (MQ=255)
            cTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGc                                                                              <  2:5575368/93‑1 (MQ=255)
             tCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGcc                                                                             >  2:5934631/1‑93 (MQ=255)
             tCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGcc                                                                             >  2:5121188/1‑93 (MQ=255)
             tCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGcc                                                                             <  2:3387476/93‑1 (MQ=255)
             tCCATATGTCCATAACGTCCGTCGGGTACAGATTGAAAAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGca                                                                             >  1:5157123/1‑92 (MQ=255)
               cATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGACCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCaa                                                                           >  1:2401233/1‑93 (MQ=255)
                atatGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCaaa                                                                          <  2:4787260/93‑1 (MQ=255)
                 tatGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAAc                                                                         <  2:3434217/93‑1 (MQ=255)
                 tatGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAAc                                                                         <  1:2347205/93‑1 (MQ=255)
                 tatGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAAc                                                                         <  2:4142743/93‑1 (MQ=255)
                 tatGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAAc                                                                         >  1:1557188/1‑93 (MQ=255)
                    gTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTa                                                                      <  1:964995/93‑1 (MQ=255)
                    gTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTa                                                                      <  2:1920676/93‑1 (MQ=255)
                     tCCATAACGTCCGTCGGGTACAGATTGAACAACTGTTGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCAAAACGTag                                                                     >  2:638113/1‑93 (MQ=255)
                      ccATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTaga                                                                    <  1:145734/93‑1 (MQ=255)
                       cATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTagaa                                                                   <  1:5130600/93‑1 (MQ=255)
                       cATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTagaa                                                                   <  2:2819758/93‑1 (MQ=255)
                        aTAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTagaag                                                                  >  1:388943/1‑93 (MQ=255)
                         tAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTagaaga                                                                 <  1:215902/93‑1 (MQ=255)
                            cgtccgtcGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGt                                                              <  1:982531/93‑1 (MQ=255)
                            cgtccgtcGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGt                                                              >  2:3133656/1‑93 (MQ=255)
                            cgtccgtcGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGt                                                              <  1:3044404/93‑1 (MQ=255)
                            cgtccgtcGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGt                                                              <  1:4989552/93‑1 (MQ=255)
                            cgtccgtcGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGt                                                              <  1:2991639/93‑1 (MQ=255)
                              tccgtcGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGttt                                                            >  1:3837047/1‑93 (MQ=255)
                              tccgtcGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGttt                                                            <  1:4138053/93‑1 (MQ=255)
                              tccgtcGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGttt                                                            >  1:4212817/1‑93 (MQ=255)
                              tccgtcGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGttt                                                            <  1:1011613/93‑1 (MQ=255)
                              tccgtcGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGttt                                                            <  1:3205197/93‑1 (MQ=255)
                               ccgtcGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGtttt                                                           >  1:6066776/1‑93 (MQ=255)
                                cgtcGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTc                                                          >  1:274028/1‑93 (MQ=255)
                                    gggTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCAcc                                                      <  2:6111948/93‑1 (MQ=255)
                                    gggTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCAcc                                                      <  1:4540631/93‑1 (MQ=255)
                                      gTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGc                                                    <  1:5775530/93‑1 (MQ=255)
                                      gTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGc                                                    <  1:2204825/93‑1 (MQ=255)
                                       tACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCt                                                   >  2:439538/1‑93 (MQ=255)
                                        aCAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTg                                                  >  2:6059647/1‑93 (MQ=255)
                                        aCAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTg                                                  <  2:2609365/93‑1 (MQ=255)
                                         cAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCGACCGCTGc                                                 <  2:1322422/93‑1 (MQ=255)
                                         cAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGc                                                 <  1:4602621/93‑1 (MQ=255)
                                         cAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGc                                                 <  1:5634144/93‑1 (MQ=255)
                                         cAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGc                                                 >  1:2545098/1‑93 (MQ=255)
                                           gATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCgg                                               <  1:736614/93‑1 (MQ=255)
                                            aTTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGt                                              <  2:6273350/93‑1 (MQ=255)
                                              tGAACACCTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCt                                            <  2:521176/93‑1 (MQ=255)
                                              tGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCt                                            <  1:1940168/93‑1 (MQ=255)
                                              tGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCt                                            <  2:5368867/93‑1 (MQ=255)
                                               gAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTg                                           >  1:208780/1‑93 (MQ=255)
                                                aacaacTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTgg                                          <  2:770862/93‑1 (MQ=255)
                                                aacaacTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTgg                                          <  2:3020797/93‑1 (MQ=255)
                                                 acaacTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGa                                         <  1:1226675/93‑1 (MQ=255)
                                                 acaacTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTCGa                                         >  1:4661228/1‑93 (MQ=255)
                                                  caacTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGANGTTTTCCACCGCTNCGGTCTGGaa                                        >  2:2426019/1‑93 (MQ=255)
                                                  caacTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGaa                                        <  2:4957007/93‑1 (MQ=255)
                                                    acTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAgg                                      <  1:5813735/93‑1 (MQ=255)
                                                        tCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTa                                  >  2:5445341/1‑93 (MQ=255)
                                                        tCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTa                                  <  1:3353858/93‑1 (MQ=255)
                                                         cGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTaa                                 <  1:1348276/93‑1 (MQ=255)
                                                         cGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTaa                                 <  1:6235470/93‑1 (MQ=255)
                                                           gctccTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGt                               <  2:2732010/89‑1 (MQ=255)
                                                           cccACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCCGGAAGGATTAAGt                               >  1:3601513/1‑93 (MQ=255)
                                                            ccgcTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGtt                              <  1:2905473/90‑1 (MQ=255)
                                                             cACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTg                             <  2:2256692/93‑1 (MQ=255)
                                                             cACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTg                             <  2:484799/93‑1 (MQ=255)
                                                             cACTTGCTGGCGCGTGGTGGCAAGTTATGGTTGGCGTGAAACGCCAAAAGAAGAAGAAGTTTTCCACCGCTGCGGTTTGACAGGATTAAGTTg                             >  2:3992636/1‑93 (MQ=255)
                                                              aCTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGc                            >  2:3490096/1‑93 (MQ=255)
                                                              aCTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGc                            <  1:5741965/93‑1 (MQ=255)
                                                               cTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGcc                           >  1:2736237/1‑93 (MQ=255)
                                                                tttCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCg                          <  2:2947862/93‑1 (MQ=255)
                                                                     ggCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGgatga                     <  1:5237439/93‑1 (MQ=255)
                                                                     ggCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGgatga                     >  1:4344746/1‑93 (MQ=255)
                                                                     ggCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGgatga                     >  2:1375027/1‑93 (MQ=255)
                                                                       cgcgTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGatgatg                   <  2:1540340/93‑1 (MQ=255)
                                                                        gcgTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATgg                  >  2:3749014/1‑93 (MQ=255)
                                                                          gtggtgGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCGGGATGATGGaa                >  1:4857039/1‑93 (MQ=255)
                                                                          gtggtgGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGaa                >  2:1720785/1‑93 (MQ=255)
                                                                          gtggtgGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGaa                >  2:2279660/1‑93 (MQ=255)
                                                                            ggtggCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAAc              >  2:3948544/1‑93 (MQ=255)
                                                                              tggCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAAcct            <  2:5079430/93‑1 (MQ=255)
                                                                              tggCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGCTGCCGGATGATGGAAAcct            >  2:5822475/1‑93 (MQ=255)
                                                                                gCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAAcctcc          <  1:1897813/93‑1 (MQ=255)
                                                                                gCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAAcctcc          >  2:4071527/1‑93 (MQ=255)
                                                                                  aaGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCctt        >  1:3958205/1‑93 (MQ=255)
                                                                                   aGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCcttc       <  2:2873821/93‑1 (MQ=255)
                                                                                        tGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTAt  >  2:2236135/1‑93 (MQ=255)
                                                                                             |                                                                                       
GCACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTT‑GCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTAT  >  NC_000913/3930475‑3930654

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTT‑GCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCAT  >  NC_000913/3930475‑3930661
                                                                                              |                                                                                             
GCACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTT‑G                                                                                               <  SRR4302533.1733516/94‑1 (MQ=60)
GCACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTT‑G                                                                                               <  SRR4302533.1501163/94‑1 (MQ=60)
 CACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTT‑GG                                                                                              <  SRR4302533.2251454/94‑1 (MQ=60)
 CACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTT‑GG                                                                                              <  SRR4302533.2803256/94‑1 (MQ=60)
 CACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTT‑GG                                                                                              <  SRR4302533.1639097/94‑1 (MQ=60)
  ACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTT‑Ggc                                                                                             <  SRR4302533.1287250/94‑3 (MQ=60)
  ACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTT‑Ggc                                                                                             <  SRR4302533.376377/94‑3 (MQ=60)
   CCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCAGGCGCGTGGTGGCAAGTTATGGTT‑Ggcg                                                                                            >  SRR4302533.5832345/1‑91 (MQ=60)
    CGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGA                                                                                            >  SRR4302533.280059/1‑94 (MQ=60)
    CGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGA                                                                                            >  SRR4302533.5675560/1‑94 (MQ=60)
    CGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGA                                                                                            >  SRR4302533.2600261/1‑94 (MQ=60)
     GAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAG                                                                                           <  SRR4302533.276721/94‑1 (MQ=60)
     GAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAG                                                                                           >  SRR4302533.5311488/1‑94 (MQ=60)
       AGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAA                                                                                         <  SRR4302533.374474/94‑1 (MQ=60)
         ACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAAC                                                                                       >  SRR4302533.5503212/1‑94 (MQ=60)
         ACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAAC                                                                                       <  SRR4302533.5445042/94‑1 (MQ=60)
         ACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAAC                                                                                       <  SRR4302533.5173744/94‑1 (MQ=60)
           GCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGC                                                                                     <  SRR4302533.1842458/94‑1 (MQ=60)
            CTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCC                                                                                    <  SRR4302533.2210766/94‑1 (MQ=60)
            CTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAAAAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCA                                                                                    >  SRR4302533.5188258/1‑94 (MQ=60)
             TCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCA                                                                                   >  SRR4302533.31634/1‑94 (MQ=60)
              CCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGACCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAA                                                                                  >  SRR4302533.2416062/1‑94 (MQ=60)
                ATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAAC                                                                                >  SRR4302533.1566560/1‑94 (MQ=60)
                ATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAAC                                                                                <  SRR4302533.6094212/94‑1 (MQ=60)
                 TATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACG                                                                               <  SRR4302533.2361729/94‑1 (MQ=60)
                  ATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGT                                                                              <  SRR4302533.4828420/94‑1 (MQ=60)
                   TGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTA                                                                             <  SRR4302533.2992344/94‑1 (MQ=60)
                    GTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAG                                                                            <  SRR4302533.970659/94‑1 (MQ=60)
                    GTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAG                                                                            <  SRR4302533.5893210/94‑1 (MQ=60)
                    GTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAG                                                                            <  SRR4302533.4193800/94‑1 (MQ=60)
                     TCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGA                                                                           >  SRR4302533.3824947/1‑94 (MQ=60)
                      CCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAA                                                                          >  SRR4302533.536299/1‑94 (MQ=60)
                      CCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAA                                                                          <  SRR4302533.146664/94‑1 (MQ=60)
                       CATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGTGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAG                                                                         <  SRR4302533.4471014/94‑1 (MQ=60)
                       CATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAG                                                                         >  SRR4302533.391292/1‑94 (MQ=60)
                       CATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAG                                                                         <  SRR4302533.5161593/94‑1 (MQ=60)
                       CATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAG                                                                         >  SRR4302533.5496626/1‑94 (MQ=60)
                         TAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAA                                                                       <  SRR4302533.217260/94‑1 (MQ=60)
                            CGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTT                                                                    <  SRR4302533.5019802/94‑1 (MQ=60)
                            CGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTT                                                                    <  SRR4302533.3009934/94‑1 (MQ=60)
                            CGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTT                                                                    >  SRR4302533.2754921/1‑94 (MQ=60)
                            CGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTT                                                                    <  SRR4302533.3063039/94‑1 (MQ=60)
                            CGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTT                                                                    <  SRR4302533.988288/94‑1 (MQ=60)
                             GTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTT                                                                   >  SRR4302533.3860489/1‑94 (MQ=60)
                             GTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTT                                                                   >  SRR4302533.5672018/1‑94 (MQ=60)
                             GTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTT                                                                   >  SRR4302533.3633825/1‑94 (MQ=60)
                             GTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTT                                                                   <  SRR4302533.3884508/94‑1 (MQ=60)
                             GTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTT                                                                   >  SRR4302533.4238301/1‑94 (MQ=60)
                              TCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTT                                                                  <  SRR4302533.4163157/94‑1 (MQ=60)
                              TCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTT                                                                  >  SRR4302533.6103492/1‑94 (MQ=60)
                              TCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTT                                                                  <  SRR4302533.801173/94‑1 (MQ=60)
                              TCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTT                                                                  <  SRR4302533.1017534/94‑1 (MQ=60)
                              TCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTT                                                                  <  SRR4302533.3224930/94‑1 (MQ=60)
                               CCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTC                                                                 >  SRR4302533.275695/1‑94 (MQ=60)
                                CGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCC                                                                >  SRR4302533.5386519/1‑94 (MQ=60)
                                    GGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCG                                                            <  SRR4302533.3977464/94‑1 (MQ=60)
                                    GGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCG                                                            <  SRR4302533.3977463/94‑1 (MQ=60)
                                    GGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCG                                                            <  SRR4302533.4568123/94‑1 (MQ=60)
                                    GGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCG                                                            <  SRR4302533.239879/94‑1 (MQ=60)
                                      GTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCT                                                          <  SRR4302533.2218419/94‑1 (MQ=60)
                                      GTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCT                                                          <  SRR4302533.357355/94‑1 (MQ=60)
                                      GTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCT                                                          <  SRR4302533.5810411/94‑1 (MQ=60)
                                       TACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTG                                                         >  SRR4302533.2094488/1‑94 (MQ=60)
                                        ACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGC                                                        >  SRR4302533.2560759/1‑94 (MQ=60)
                                         CAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCG                                                       <  SRR4302533.4630504/94‑1 (MQ=60)
                                         CAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCG                                                       <  SRR4302533.5668213/94‑1 (MQ=60)
                                           GATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGT                                                     <  SRR4302533.740946/94‑1 (MQ=60)
                                           GATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGT                                                     <  SRR4302533.2870336/94‑1 (MQ=60)
                                           GATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGT                                                     >  SRR4302533.1455135/1‑94 (MQ=60)
                                              TGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTG                                                  >  SRR4302533.210100/1‑94 (MQ=60)
                                              TGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTG                                                  >  SRR4302533.301175/1‑94 (MQ=60)
                                              TGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTG                                                  <  SRR4302533.1952002/94‑1 (MQ=60)
                                               GAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGG                                                 <  SRR4302533.6159494/94‑1 (MQ=60)
                                                AACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTCGA                                                >  SRR4302533.4689475/1‑94 (MQ=60)
                                                 ACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAA                                               <  SRR4302533.1234022/94‑1 (MQ=60)
                                                  CAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAG                                              <  SRR4302533.5520151/94‑1 (MQ=60)
                                                   AACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGG                                             <  SRR4302533.3147139/94‑1 (MQ=60)
                                                   AACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGG                                             <  SRR4302533.98472/94‑1 (MQ=60)
                                                    ACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGA                                            <  SRR4302533.1085006/94‑1 (MQ=60)
                                                    ACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGA                                            <  SRR4302533.5848847/94‑1 (MQ=60)
                                                      TGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATT                                          >  SRR4302533.2534558/1‑94 (MQ=60)
                                                      TGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATT                                          >  SRR4302533.4591752/1‑94 (MQ=60)
                                                       GTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTA                                         <  SRR4302533.4327884/94‑1 (MQ=60)
                                                        TCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAA                                        <  SRR4302533.3374584/94‑1 (MQ=60)
                                                         CGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAG                                       <  SRR4302533.1356390/94‑1 (MQ=60)
                                                         CGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAG                                       <  SRR4302533.6272813/94‑1 (MQ=60)
                                                          GCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGT                                      <  SRR4302533.6289880/94‑1 (MQ=60)
                                                          GCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGT                                      >  SRR4302533.1426507/1‑94 (MQ=60)
                                                          GCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGT                                      >  SRR4302533.5107799/1‑94 (MQ=60)
                                                          GCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCCGGAAGGATTAAGT                                      >  SRR4302533.3623675/1‑94 (MQ=60)
                                                           CCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTT                                     <  SRR4302533.1704087/94‑1 (MQ=60)
                                                           CCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTT                                     <  SRR4302533.1917863/94‑1 (MQ=60)
                                                            CCGCTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTG                                    <  SRR4302533.2923316/94‑1 (MQ=60)
                                                            CCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTG                                    >  SRR4302533.232952/1‑94 (MQ=60)
                                                            CCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCAGCGGTCTGGAAGGATTAAGTTG                                    <  SRR4302533.5225298/94‑1 (MQ=60)
                                                              ACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCC                                  <  SRR4302533.5776615/94‑1 (MQ=60)
                                                              ACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCC                                  >  SRR4302533.2753021/1‑94 (MQ=60)
                                                               CTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCG                                 <  SRR4302533.4656926/94‑1 (MQ=60)
                                                                  TCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGAT                              <  SRR4302533.5127187/94‑1 (MQ=60)
                                                                   CTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATG                             <  SRR4302533.1338661/94‑1 (MQ=60)
                                                                    TGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGA                            >  SRR4302533.4370930/1‑94 (MQ=60)
                                                                     GGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGAT                           <  SRR4302533.5268928/94‑1 (MQ=60)
                                                                      GCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATG                          >  SRR4302533.1276237/1‑94 (MQ=60)
                                                                       CGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGG                         >  SRR4302533.4261694/1‑94 (MQ=60)
                                                                        GCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGA                        >  SRR4302533.5781243/1‑94 (MQ=60)
                                                                        GCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGA                        >  SRR4302533.468475/1‑94 (MQ=60)
                                                                        GCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGA                        <  SRR4302533.2627603/94‑1 (MQ=60)
                                                                         CGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCGGGATGATGGAA                       >  SRR4302533.4886473/1‑94 (MQ=60)
                                                                              TGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTC                  >  SRR4302533.2152866/1‑94 (MQ=60)
                                                                                GCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCT                <  SRR4302533.1909401/94‑1 (MQ=60)
                                                                                 CAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTT               >  SRR4302533.3982327/1‑94 (MQ=60)
                                                                                   AGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCT             <  SRR4302533.1716254/94‑1 (MQ=60)
                                                                                   AGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCT             >  SRR4302533.4000807/1‑94 (MQ=60)
                                                                                     TTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTT           <  SRR4302533.863250/94‑1 (MQ=60)
                                                                                     TTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTT           <  SRR4302533.2069222/94‑1 (MQ=60)
                                                                                     TTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTT           >  SRR4302533.4910296/1‑94 (MQ=60)
                                                                                      TATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTA          >  SRR4302533.3290262/1‑94 (MQ=60)
                                                                                       ATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTAT         <  SRR4302533.1621295/94‑1 (MQ=60)
                                                                                         GGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGT       >  SRR4302533.3871051/1‑94 (MQ=60)
                                                                                         GGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGT       <  SRR4302533.5555108/94‑1 (MQ=60)
                                                                                         GGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGT       >  SRR4302533.541332/1‑94 (MQ=60)
                                                                                         GGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCCGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGT       >  SRR4302533.4894028/1‑94 (MQ=60)
                                                                                          GTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTC      <  SRR4302533.3118455/94‑1 (MQ=60)
                                                                                          GTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTC      >  SRR4302533.1069007/1‑94 (MQ=60)
                                                                                           TG‑GCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGC    >  SRR4302533.4585700/1‑94 (MQ=60)
                                                                                              GCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCAT  <  SRR4302533.6297928/94‑1 (MQ=60)
                                                                                              GCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCAT  <  SRR4302533.3569066/94‑1 (MQ=60)
                                                                                              GCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCAT  >  SRR4302533.2873941/1‑94 (MQ=60)
                                                                                              |                                                                                             
GCACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTT‑GCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCAT  >  NC_000913/3930475‑3930661

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑