Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,933,993 G→A A30A (GCG→GCA)  rbsB → D‑ribose periplasmic binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,933,9930GA99.6% 948.8 / NA 258A30A (GCG→GCA) rbsBD‑ribose periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (140/116);  minor base C (1/0);  total (141/117)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 266 total aligned reads displayed.

TATGAACATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACTTGGCTATAACCTGG  >  NC_000913/3933903‑3934081
                                                                                          |                                                                                        
 atGAACATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACtg                                                                                       <  1:2796999/93‑1 (MQ=255)
  tGAACATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACtgg                                                                                      <  2:5885531/93‑1 (MQ=255)
   gAACATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACtggt                                                                                     <  2:3789251/93‑1 (MQ=255)
   gAACATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACtggt                                                                                     >  2:4439895/1‑93 (MQ=255)
   gAACATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACtggt                                                                                     <  2:252741/93‑1 (MQ=255)
    aaCATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACtggtg                                                                                    >  2:818589/1‑93 (MQ=255)
       aTGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGtc                                                                                 >  2:1991962/1‑93 (MQ=255)
          aaaaaaCAGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTcc                                                                              <  2:242493/93‑1 (MQ=255)
           aaaaaCTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCa                                                                             >  1:1695165/1‑93 (MQ=255)
                cTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCtt                                                                        >  2:5764571/1‑93 (MQ=255)
                 tGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTa                                                                       >  2:4702945/1‑93 (MQ=255)
                 tGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTa                                                                       >  1:1036796/1‑93 (MQ=255)
                 tGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTa                                                                       >  1:3351132/1‑93 (MQ=255)
                 tGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTa                                                                       <  2:1747920/93‑1 (MQ=255)
                 tGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGGGGTCTCCACGCTTa                                                                       >  2:3750601/1‑93 (MQ=255)
                    cTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTaaca                                                                    >  1:3831415/1‑93 (MQ=255)
                     ttcccTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTaacaa                                                                   <  2:5180191/91‑1 (MQ=255)
                     tACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTaacaa                                                                   >  2:5244287/1‑93 (MQ=255)
                     tACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTaacaa                                                                   >  1:4140770/1‑93 (MQ=255)
                      aCCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTaacaac                                                                  >  1:5010988/1‑93 (MQ=255)
                      aCCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTaacaac                                                                  >  1:762313/1‑93 (MQ=255)
                      aCCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTaacaac                                                                  <  2:2948840/93‑1 (MQ=255)
                       cccTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAAcc                                                                 <  2:1420156/93‑1 (MQ=255)
                       cccTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAAcc                                                                 >  1:1781954/1‑93 (MQ=255)
                        ccTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAAccc                                                                >  1:5106450/1‑93 (MQ=255)
                        ccTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAAccc                                                                <  2:5749584/93‑1 (MQ=255)
                        ccTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAAccc                                                                >  1:908655/1‑93 (MQ=255)
                          tGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAAGCCGt                                                              >  2:4265734/1‑93 (MQ=255)
                          tGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGt                                                              <  2:4951915/93‑1 (MQ=255)
                          tGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGt                                                              >  1:1524935/1‑93 (MQ=255)
                          tGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGt                                                              >  1:4620904/1‑93 (MQ=255)
                          tGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGt                                                              <  2:4201995/93‑1 (MQ=255)
                           ggTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGtt                                                             >  2:803937/1‑93 (MQ=255)
                            gTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTc                                                            >  2:4716892/1‑93 (MQ=255)
                            gTTTCCGCTGTGGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGGCGCCCCGCTTAACAACCCGTTc                                                            <  2:1770289/93‑1 (MQ=255)
                             tttCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCt                                                           <  1:4017558/93‑1 (MQ=255)
                              ttCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCtt                                                          >  1:181683/1‑93 (MQ=255)
                               tCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCttt                                                         <  1:5854150/93‑1 (MQ=255)
                                ccGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTg                                                        >  2:3489598/1‑93 (MQ=255)
                                ccGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTg                                                        <  2:874806/93‑1 (MQ=255)
                                  gCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTa                                                      <  2:5720640/93‑1 (MQ=255)
                                  gCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTa                                                      <  2:1036796/93‑1 (MQ=255)
                                  gCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTa                                                      <  2:4861453/93‑1 (MQ=255)
                                  gCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTa                                                      >  2:2826973/1‑93 (MQ=255)
                                   cTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTAt                                                     <  2:404170/93‑1 (MQ=255)
                                    tGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATc                                                    <  1:5619856/93‑1 (MQ=255)
                                       tGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAATAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCt                                                 >  1:5198869/1‑93 (MQ=255)
                                       tGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCt                                                 <  1:1278504/93‑1 (MQ=255)
                                       tGCGCTAAGCGCCACCGTCAGTGAGAATGCGATGGCAAAAGTCACCATCGCAGTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCt                                                 <  2:2898528/93‑1 (MQ=255)
                                         cgcTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGa                                               <  1:3101393/93‑1 (MQ=255)
                                         cgcTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGa                                               <  2:4995795/93‑1 (MQ=255)
                                            tAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCCCTGGTGGTCTCCACGCTTAACAACCCGTTCTTTTTATCGCTGaaac                                            >  2:2035813/1‑92 (MQ=255)
                                            tAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAg                                            >  1:4359168/1‑93 (MQ=255)
                                            tAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAg                                            >  1:4554863/1‑93 (MQ=255)
                                            tAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAg                                            >  2:2722483/1‑93 (MQ=255)
                                            tAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAg                                            >  1:4111217/1‑93 (MQ=255)
                                            tAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAg                                            >  1:3546135/1‑93 (MQ=255)
                                             aaGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGa                                           <  2:1195049/93‑1 (MQ=255)
                                             aaGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGa                                           <  1:6076873/93‑1 (MQ=255)
                                                 gcCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGgc                                       >  2:1508117/1‑93 (MQ=255)
                                                 gcCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGgc                                       >  2:4593466/1‑93 (MQ=255)
                                                 gcCACCGTCAGTGCGAATGCGATGGCAAAAGACACCACCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGgc                                       <  1:2495191/93‑1 (MQ=255)
                                                 gcCACAATCAGTGCGAATGCTAGGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAGCCCGTTCTTTGTATCGCTGAAAGATGgc                                       <  1:5889584/93‑1 (MQ=255)
                                                  ccACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGgcg                                      >  2:1450179/1‑93 (MQ=255)
                                                   cACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACACGTTCTTTGTATCGCTGAAAGATGgcgc                                     >  1:1362994/1‑93 (MQ=255)
                                                    aCCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGgcgcg                                    >  1:947408/1‑93 (MQ=255)
                                                      cGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCa                                  <  2:6108871/93‑1 (MQ=255)
                                                      cGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCa                                  <  2:6223389/93‑1 (MQ=255)
                                                           gTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGACag                             >  2:540406/1‑93 (MQ=255)
                                                            tGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAaga                            <  1:5679314/93‑1 (MQ=255)
                                                            tGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAaga                            >  2:645324/1‑93 (MQ=255)
                                                             gCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAagag                           >  1:1063444/1‑93 (MQ=255)
                                                             gCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAagag                           <  2:3928106/93‑1 (MQ=255)
                                                                aaTGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCg                        >  2:1709524/1‑93 (MQ=255)
                                                                aaTGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCg                        >  1:234789/1‑93 (MQ=255)
                                                                aaTGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCg                        >  1:438664/1‑93 (MQ=255)
                                                                aaTGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCg                        <  1:480298/93‑1 (MQ=255)
                                                                 aTGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCgg                       >  1:1820621/1‑93 (MQ=255)
                                                                  tGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGa                      >  1:1171791/1‑93 (MQ=255)
                                                                  tGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGa                      >  1:4490191/1‑93 (MQ=255)
                                                                  tGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGa                      <  2:3054443/93‑1 (MQ=255)
                                                                     gATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATaa                   >  1:5364384/1‑93 (MQ=255)
                                                                     gATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATaa                   >  1:4268349/1‑93 (MQ=255)
                                                                      aTGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATaaa                  >  1:6169668/1‑93 (MQ=255)
                                                                          cAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACTTg              <  1:4758187/93‑1 (MQ=255)
                                                                          cAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACTTg              <  2:3642696/93‑1 (MQ=255)
                                                                          cAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACTTg              <  1:2809764/93‑1 (MQ=255)
                                                                           aaaaGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACTTgg             >  1:1919240/1‑93 (MQ=255)
                                                                            aaaGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACTTGGc            >  2:2089005/1‑93 (MQ=255)
                                                                            aaaGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACTTGGc            <  1:4560810/93‑1 (MQ=255)
                                                                            aaaGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACTTGGc            <  2:2877875/93‑1 (MQ=255)
                                                                             aaGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACTTGGCt           <  1:3920553/93‑1 (MQ=255)
                                                                              aGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACTTGGCta          >  1:2100029/1‑93 (MQ=255)
                                                                               gACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACTTGGCtat         <  2:3213731/93‑1 (MQ=255)
                                                                                 cacCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGGGCAGAAAGAGGCGGATAAACTTGGCTCTaa       >  1:5964239/1‑93 (MQ=255)
                                                                                  acCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACTTGGCTATAAc      <  1:2956263/93‑1 (MQ=255)
                                                                                   ccATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACTTGGCTATAAcc     <  1:5325705/93‑1 (MQ=255)
                                                                                     aTCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGATGAAAGATGGCGCGCAGAAAGAGGCGGATAAACTTGGCTATAACCtg   <  2:441444/93‑1 (MQ=255)
                                                                                      tCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACTTGGCTATAACCtgg  <  2:2994683/93‑1 (MQ=255)
                                                                                      tCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACTTGGCTATAACCtgg  <  2:2298729/93‑1 (MQ=255)
                                                                                          |                                                                                        
TATGAACATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACTTGGCTATAACCTGG  >  NC_000913/3933903‑3934081

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AATATGAACATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACTTGGCTATAACCTGGTGGTG  >  NC_000913/3933901‑3934086
                                                                                            |                                                                                             
AATATGAACATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCAC                                                                                              >  SRR4302533.77549/1‑94 (MQ=60)
AATATGAACATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCAC                                                                                              >  SRR4302533.2709333/1‑94 (MQ=60)
 ATATGAACATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACT                                                                                             >  SRR4302533.2609157/1‑94 (MQ=60)
 ATATGAACATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACT                                                                                             >  SRR4302533.78772/1‑94 (MQ=60)
 ATATGAACATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACT                                                                                             <  SRR4302533.1378286/94‑1 (MQ=60)
  TATGAACATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTG                                                                                            >  SRR4302533.5348049/1‑94 (MQ=60)
   ATGAACATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGG                                                                                           <  SRR4302533.2814202/94‑1 (MQ=60)
   ATGAACATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGG                                                                                           <  SRR4302533.2814203/94‑1 (MQ=60)
     GAACATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTG                                                                                         <  SRR4302533.1801911/94‑1 (MQ=60)
      AACATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGG                                                                                        >  SRR4302533.5935730/1‑94 (MQ=60)
      AACATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGG                                                                                        <  SRR4302533.511083/94‑1 (MQ=60)
       ACATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGT                                                                                       >  SRR4302533.3323465/1‑94 (MQ=60)
        CATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTC                                                                                      >  SRR4302533.6235777/1‑94 (MQ=60)
        CATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGCGGTC                                                                                      >  SRR4302533.4570421/1‑94 (MQ=60)
          TGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTC                                                                                    >  SRR4302533.43930/1‑94 (MQ=60)
            AAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCA                                                                                  >  SRR4302533.1705432/1‑94 (MQ=60)
            AAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCA                                                                                  >  SRR4302533.1082574/1‑94 (MQ=60)
              AAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACG                                                                                <  SRR4302533.4620268/94‑1 (MQ=60)
              AAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACG                                                                                <  SRR4302533.5391729/94‑1 (MQ=60)
                AACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCT                                                                              >  SRR4302533.1549677/1‑94 (MQ=60)
                 ACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTT                                                                             >  SRR4302533.5168986/1‑94 (MQ=60)
                  CTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTA                                                                            >  SRR4302533.2939147/1‑94 (MQ=60)
                  CTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTA                                                                            >  SRR4302533.3371833/1‑94 (MQ=60)
                  CTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTA                                                                            >  SRR4302533.1042890/1‑94 (MQ=60)
                     GCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACA                                                                         >  SRR4302533.3854837/1‑94 (MQ=60)
                     GCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATAGCACTGGTGGTCTCCACGCTTCACA                                                                         >  SRR4302533.6014088/1‑94 (MQ=60)
                      CTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAA                                                                        >  SRR4302533.4165883/1‑94 (MQ=60)
                      CTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAA                                                                        <  SRR4302533.3427103/94‑1 (MQ=60)
                      CTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAA                                                                        >  SRR4302533.1812071/1‑94 (MQ=60)
                       TACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAAC                                                                       >  SRR4302533.766800/1‑94 (MQ=60)
                       TACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAAC                                                                       >  SRR4302533.3719628/1‑94 (MQ=60)
                       TACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAAC                                                                       >  SRR4302533.5041348/1‑94 (MQ=60)
                       TACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAAC                                                                       >  SRR4302533.5052388/1‑94 (MQ=60)
                       TACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAAC                                                                       >  SRR4302533.1706959/1‑94 (MQ=60)
                        ACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACC                                                                      >  SRR4302533.1792795/1‑94 (MQ=60)
                         CCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCC                                                                     >  SRR4302533.445152/1‑94 (MQ=60)
                         CCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCC                                                                     >  SRR4302533.913984/1‑94 (MQ=60)
                         CCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCC                                                                     >  SRR4302533.5137299/1‑94 (MQ=60)
                          CCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCG                                                                    >  SRR4302533.4089265/1‑94 (MQ=60)
                          CCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCG                                                                    >  SRR4302533.4481835/1‑94 (MQ=60)
                           CTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGT                                                                   >  SRR4302533.1534115/1‑94 (MQ=60)
                           CTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGT                                                                   >  SRR4302533.150908/1‑94 (MQ=60)
                           CTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGT                                                                   >  SRR4302533.4648910/1‑94 (MQ=60)
                           CTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGT                                                                   >  SRR4302533.6144013/1‑94 (MQ=60)
                             GGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTC                                                                 <  SRR4302533.4928032/94‑1 (MQ=60)
                              GTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCT                                                                <  SRR4302533.5369358/94‑1 (MQ=60)
                              GTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCT                                                                <  SRR4302533.1926476/94‑1 (MQ=60)
                               TTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTT                                                               >  SRR4302533.182814/1‑94 (MQ=60)
                               TTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTT                                                               <  SRR4302533.4042018/94‑1 (MQ=60)
                                TTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTT                                                              >  SRR4302533.5662103/1‑94 (MQ=60)
                                 TCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTG                                                             >  SRR4302533.5839520/1‑94 (MQ=60)
                                 TCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTG                                                             <  SRR4302533.5889542/94‑1 (MQ=60)
                                 TCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTG                                                             <  SRR4302533.1879634/94‑1 (MQ=60)
                                  CCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGT                                                            >  SRR4302533.703246/1‑94 (MQ=60)
                                   CGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTA                                                           >  SRR4302533.152518/1‑94 (MQ=60)
                                     CTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATC                                                         <  SRR4302533.4744394/94‑1 (MQ=60)
                                     CTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATC                                                         >  SRR4302533.4349127/1‑94 (MQ=60)
                                     CTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATC                                                         >  SRR4302533.4331193/1‑94 (MQ=60)
                                      TGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCG                                                        >  SRR4302533.4578750/1‑94 (MQ=60)
                                      TGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCG                                                        <  SRR4302533.5653822/94‑1 (MQ=60)
                                      TGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCG                                                        >  SRR4302533.1335271/1‑94 (MQ=60)
                                        TTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAATAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCT                                                      >  SRR4302533.5230217/1‑94 (MQ=60)
                                         TGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTG                                                     <  SRR4302533.1286189/94‑1 (MQ=60)
                                           CGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAA                                                   <  SRR4302533.3120460/94‑1 (MQ=60)
                                           CGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAA                                                   <  SRR4302533.4789771/94‑1 (MQ=60)
                                             CTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAG                                                 >  SRR4302533.4582489/1‑94 (MQ=60)
                                             CTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAG                                                 >  SRR4302533.3567992/1‑94 (MQ=60)
                                             CTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAG                                                 >  SRR4302533.4136205/1‑94 (MQ=60)
                                             CTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAG                                                 <  SRR4302533.4629586/94‑1 (MQ=60)
                                             CTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAG                                                 >  SRR4302533.3897512/1‑94 (MQ=60)
                                             CTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAG                                                 >  SRR4302533.4385480/1‑94 (MQ=60)
                                             CTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTCGTATCGCTGAAAG                                                 <  SRR4302533.3427468/94‑1 (MQ=60)
                                               AAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGAT                                               <  SRR4302533.6113625/94‑1 (MQ=60)
                                                AGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATG                                              <  SRR4302533.1754623/94‑1 (MQ=60)
                                                   GCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCG                                           >  SRR4302533.4035101/1‑94 (MQ=60)
                                                   GCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCG                                           <  SRR4302533.2400710/94‑1 (MQ=60)
                                                   GCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCACCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCG                                           <  SRR4302533.2510584/94‑1 (MQ=60)
                                                   GCCACAATCAGTGCGAATGCTAGGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAGCCCGTTCTTTGTATCGCTGAAAGATGGCG                                           <  SRR4302533.5925165/94‑1 (MQ=60)
                                                    CCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGAGC                                          >  SRR4302533.997050/1‑94 (MQ=60)
                                                    CCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACACGTTCTTTGTATCGCTGAAAGATGGCGC                                          >  SRR4302533.1371182/1‑94 (MQ=60)
                                                     CACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCG                                         >  SRR4302533.952978/1‑94 (MQ=60)
                                                     CACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCG                                         >  SRR4302533.2481057/1‑94 (MQ=60)
                                                       CCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCA                                       >  SRR4302533.4075467/1‑94 (MQ=60)
                                                             GTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGA                                 <  SRR4302533.3098591/94‑1 (MQ=60)
                                                             GTGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGA                                 >  SRR4302533.5494798/1‑94 (MQ=60)
                                                              TGCGGATGCGAGGGCAAAAGACCACTTCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAG                                <  SRR4302533.1354972/94‑1 (MQ=60)
                                                              TGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAG                                >  SRR4302533.1069693/1‑94 (MQ=60)
                                                              TGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAG                                <  SRR4302533.5713615/94‑1 (MQ=60)
                                                              TGCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAG                                >  SRR4302533.2833401/1‑94 (MQ=60)
                                                               GCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGG                               >  SRR4302533.5692319/1‑94 (MQ=60)
                                                               GCGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGG                               <  SRR4302533.949017/94‑1 (MQ=60)
                                                                CGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGC                              <  SRR4302533.4257679/94‑1 (MQ=60)
                                                                CGAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGC                              <  SRR4302533.3039831/94‑1 (MQ=60)
                                                                 GAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCG                             >  SRR4302533.236237/1‑94 (MQ=60)
                                                                 GAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCG                             <  SRR4302533.415421/94‑1 (MQ=60)
                                                                 GAATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCG                             >  SRR4302533.441279/1‑94 (MQ=60)
                                                                  AATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGG                            <  SRR4302533.483169/94‑1 (MQ=60)
                                                                  AATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGG                            >  SRR4302533.1831742/1‑94 (MQ=60)
                                                                   ATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGA                           >  SRR4302533.2235129/1‑94 (MQ=60)
                                                                   ATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGA                           >  SRR4302533.1178824/1‑94 (MQ=60)
                                                                   ATGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGA                           >  SRR4302533.4517384/1‑94 (MQ=60)
                                                                    TGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGAT                          <  SRR4302533.4582713/94‑1 (MQ=60)
                                                                    TGCGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGAT                          <  SRR4302533.2703421/94‑1 (MQ=60)
                                                                      CGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAA                        >  SRR4302533.4294047/1‑94 (MQ=60)
                                                                      CGATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAA                        >  SRR4302533.5396607/1‑94 (MQ=60)
                                                                       GATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAA                       >  SRR4302533.6206752/1‑94 (MQ=60)
                                                                        ATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAAC                      <  SRR4302533.1073751/94‑1 (MQ=60)
                                                                        ATGGCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAAC                      >  SRR4302533.4748078/1‑94 (MQ=60)
                                                                           GCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACTTG                   <  SRR4302533.3827147/94‑1 (MQ=60)
                                                                           GCAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACTTG                   <  SRR4302533.835156/94‑1 (MQ=60)
                                                                            CAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACTTGG                  <  SRR4302533.4787055/94‑1 (MQ=60)
                                                                            CAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACTTGG                  >  SRR4302533.1930956/1‑94 (MQ=60)
                                                                            CAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACTTGG                  <  SRR4302533.2827054/94‑1 (MQ=60)
                                                                            CAAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACTTGG                  <  SRR4302533.1830454/94‑1 (MQ=60)
                                                                             AAAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACTTGGC                 <  SRR4302533.4201695/94‑1 (MQ=60)
                                                                              AAAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACTTGGCT                <  SRR4302533.4588473/94‑1 (MQ=60)
                                                                               AAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACTTGGCTA               >  SRR4302533.2112982/1‑94 (MQ=60)
                                                                               AAGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACTTGGCTA               <  SRR4302533.3944494/94‑1 (MQ=60)
                                                                                AGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACTTGGCTAT              >  SRR4302533.1067999/1‑94 (MQ=60)
                                                                                AGACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACTTGGCTAT              >  SRR4302533.4898186/1‑94 (MQ=60)
                                                                                  ACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGGGCAGAAAGAGGCGGATAAACTTGGCTCTAA            >  SRR4302533.6000327/1‑94 (MQ=60)
                                                                                  ACACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACTTGGCTATAA            >  SRR4302533.6000328/1‑94 (MQ=60)
                                                                                    ACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACTTGGCTATAACC          >  SRR4302533.2064012/1‑94 (MQ=60)
                                                                                    ACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACTTGGCTATAACC          <  SRR4302533.1235724/94‑1 (MQ=60)
                                                                                    ACCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACTTGGCTATAACC          <  SRR4302533.2974335/94‑1 (MQ=60)
                                                                                     CCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACTTGGCTATAACCT         <  SRR4302533.5745081/94‑1 (MQ=60)
                                                                                     CCATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACTTGGCTATAACCT         <  SRR4302533.5357622/94‑1 (MQ=60)
                                                                                      CATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACTTGGCTATAACCTG        >  SRR4302533.5994127/1‑94 (MQ=60)
                                                                                      CATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACTTGGCTATAACCTG        >  SRR4302533.3995027/1‑94 (MQ=60)
                                                                                       ATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACTTGGCTATAACCTGG       <  SRR4302533.5919466/94‑1 (MQ=60)
                                                                                       ATCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACTTGGCTATAACCTGG       <  SRR4302533.5259495/94‑1 (MQ=60)
                                                                                        TCGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACTTGGCTATAACCTGGT      <  SRR4302533.2068118/94‑1 (MQ=60)
                                                                                         CGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACTTGGCTATAACCTGGTG     >  SRR4302533.4347682/1‑94 (MQ=60)
                                                                                         CGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACTTGGCTATAACCTGGTG     >  SRR4302533.1854489/1‑94 (MQ=60)
                                                                                         CGCACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACTTGGCTATAACCTGGTG     >  SRR4302533.4935187/1‑94 (MQ=60)
                                                                                           CACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACTTGGCTATAACCTGGTGGT   >  SRR4302533.2728871/1‑94 (MQ=60)
                                                                                           CACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACTTGGCTATAACCTGGTGGG   >  SRR4302533.6233364/1‑94 (MQ=60)
                                                                                            ACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACTTGGCTATAACCTGGTGGTG  <  SRR4302533.4969816/94‑1 (MQ=60)
                                                                                            ACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACTTGGCTATAACCTGGTGGTG  <  SRR4302533.3801244/94‑1 (MQ=60)
                                                                                            ACTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACTTGGCTATAACCTGGTGGTG  <  SRR4302533.1020141/94‑1 (MQ=60)
                                                                                            |                                                                                             
AATATGAACATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACTTGGCTATAACCTGGTGGTG  >  NC_000913/3933901‑3934086

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑