Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,934,526	T→C	V208A (GTT→GCT)	rbsB →	D‑ribose periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,934,526	0	T	C	99.6%	835.7 / NA	257	V208A (GTT→GCT)	rbsB	D‑ribose periplasmic binding protein
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (147/109); minor base G (1/0); total (148/109)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 259 total aligned reads displayed.

AGTTTAATGTTCTTGCCAGCCAGCCAGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGTTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCGGATGTGATGGTCGTCG  >  NC_000913/3934436‑3934615

  tttAATGTTCTTGCCAGCCAGCCGGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTATTGACCGCTCATCCGGATGCTCAg                                                                                       >  2:862926/1‑93 (MQ=255)

   ttAATGTTCTTGCCAGCCAGCCGGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAgg                                                                                      >  2:47537/1‑93 (MQ=255)

    tAATGTTCTTGCCAGCCAGCCGGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGc                                                                                     <  1:777830/93‑1 (MQ=255)

    tAATGTTCTTGCCAGCCAGCCGGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGc                                                                                     <  1:1689128/93‑1 (MQ=255)

     aaTGTTCTTGCCAGCCAGCCGGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCt                                                                                    >  1:2784205/1‑93 (MQ=255)

     aaTGTTCTTGCCAGCCAGCCGGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCt                                                                                    <  2:1495007/93‑1 (MQ=255)

      aTGTTCTTGCCAGCCAGCCGGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTg                                                                                   <  1:1235723/93‑1 (MQ=255)

       tGTTCTTGCCAGCCAGCCGGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGt                                                                                  >  2:1862796/1‑93 (MQ=255)

          tCTTGCCAGCCAGCCGGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTAtt                                                                               >  2:6033736/1‑93 (MQ=255)

           cTTGCCAGCCAGCCGGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTc                                                                              <  2:1805747/93‑1 (MQ=255)

            ttGCCAGCCAGCCGGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTcg                                                                             <  2:1389702/93‑1 (MQ=255)

            ttGCCAGCCAGCCGGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGGATTcg                                                                             >  2:1169216/1‑93 (MQ=255)

             tGCCAGCCAGCCGGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCCCAGGCTGTATTcgc                                                                            <  2:3849029/93‑1 (MQ=255)

              gccagccagccgGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTcgcg                                                                           >  2:4023686/1‑93 (MQ=255)

                  gccagccggcAGATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGa                                                                       >  1:4838841/1‑93 (MQ=255)

                    cagccggcAGATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAat                                                                     >  1:512682/1‑93 (MQ=255)

                      gccggcAGATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAatga                                                                   <  2:900416/93‑1 (MQ=255)

                      gccggcAGATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAatga                                                                   >  2:5503987/1‑93 (MQ=255)

                       ccggcagATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGActgat                                                                  >  1:2071894/4‑93 (MQ=255)

                        cggcagATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAatgatg                                                                 <  2:4348069/91‑1 (MQ=255)

                         ggcagATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAAtgatga                                                                >  1:5509628/2‑93 (MQ=255)

                         ggcagATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAAtgatga                                                                <  1:4232853/92‑1 (MQ=255)

                          gcagATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGaa                                                               >  1:1491803/1‑93 (MQ=255)

                          gcagATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGaa                                                               >  2:2801478/1‑93 (MQ=255)

                           cagATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGaaa                                                              <  2:1534695/93‑1 (MQ=255)

                           cagATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGaaa                                                              >  1:6103942/1‑93 (MQ=255)

                           cagATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGaaa                                                              <  1:598532/93‑1 (MQ=255)

                             gATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATg                                                            >  2:713326/1‑93 (MQ=255)

                               ttttGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGgc                                                          <  2:3716231/93‑1 (MQ=255)

                               ttttGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGgc                                                          >  2:1677122/1‑93 (MQ=255)

                                tttGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGgcg                                                         <  1:3242515/93‑1 (MQ=255)

                                tttGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGgcg                                                         >  1:6262718/1‑93 (MQ=255)

                                 ttGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGgcgc                                                        <  2:4223921/93‑1 (MQ=255)

                                  tGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCt                                                       >  1:5297560/1‑93 (MQ=255)

                                   gATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTg                                                      <  1:4031439/93‑1 (MQ=255)

                                    aTCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTgg                                                     >  1:3805444/1‑93 (MQ=255)

                                    aTCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTgg                                                     <  2:4707320/93‑1 (MQ=255)

                                    aTCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTgg                                                     <  1:2195030/93‑1 (MQ=255)

                                     tCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTggg                                                    >  1:4670031/1‑93 (MQ=255)

                                     tCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTggg                                                    <  2:5659670/93‑1 (MQ=255)

                                      cGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGgc                                                   >  1:2960753/1‑93 (MQ=255)

                                      cGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGgc                                                   <  2:862097/93‑1 (MQ=255)

                                        cATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCTGAATGATGAAATGGCGCTGGgcgc                                                 >  2:6181565/1‑93 (MQ=255)

                                        cATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGgcgc                                                 >  1:671136/1‑93 (MQ=255)

                                         aTTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGgcgcg                                                >  1:1845298/1‑93 (MQ=255)

                                         aTTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGgcgcg                                                >  2:1597623/1‑93 (MQ=255)

                                          ttAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGgcgcgc                                               >  1:1118809/1‑93 (MQ=255)

                                          ttAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGgcgcgc                                               >  2:5907638/1‑93 (MQ=255)

                                          ttAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGgcgcgc                                               <  1:3555530/93‑1 (MQ=255)

                                          ttAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGgcgcgc                                               <  2:4188055/93‑1 (MQ=255)

                                          ttAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGgcgcgc                                               <  2:2444041/93‑1 (MQ=255)

                                            aaaGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTg                                             >  1:1389861/1‑93 (MQ=255)

                                             aaGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTgc                                            >  1:4001012/1‑93 (MQ=255)

                                              aGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTgcg                                           >  1:5218756/1‑93 (MQ=255)

                                               ggTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTgcgc                                          >  1:4803224/1‑93 (MQ=255)

                                                gTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTgcgcg                                         >  2:5785/1‑93 (MQ=255)

                                                gTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTgcgcg                                         >  1:1062716/1‑93 (MQ=255)

                                                 tttGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTgcgcgc                                        >  1:4510229/1‑93 (MQ=255)

                                                   tGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCAc                                      <  2:3374398/93‑1 (MQ=255)

                                                     aaCGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTg                                    >  1:4952438/1‑93 (MQ=255)

                                                      aCGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGc                                   >  2:3289584/1‑93 (MQ=255)

                                                       cGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCa                                  <  2:561439/93‑1 (MQ=255)

                                                        gTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCaa                                 >  2:2295992/1‑93 (MQ=255)

                                                         tAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCaaa                                >  1:1590436/1‑93 (MQ=255)

                                                          aaTGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAAc                               >  1:5367346/1‑93 (MQ=255)

                                                          aaTGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAAc                               <  1:5436156/93‑1 (MQ=255)

                                                           aTGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACt                              >  1:3511627/1‑93 (MQ=255)

                                                              cAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGcc                           <  2:3632798/93‑1 (MQ=255)

                                                              cAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGcc                           >  2:2098961/1‑93 (MQ=255)

                                                              cAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATGCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGcc                           <  2:4094014/93‑1 (MQ=255)

                                                                gAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCgg                         >  1:5809163/1‑93 (MQ=255)

                                                                 aaCCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGt                        >  1:3047891/1‑93 (MQ=255)

                                                                    cTGTTGCCCGCTCATCCGGATGCTCAGGCTGTATTCGCGCCGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCACACTGCCGGTaaa                     >  1:5876325/1‑93 (MQ=255)

                                                                    cTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTaaa                     >  1:2687510/1‑93 (MQ=255)

                                                                     tGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAAt                    >  1:5653851/1‑93 (MQ=255)

                                                                     tGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAAt                    >  1:3280667/1‑93 (MQ=255)

                                                                     tGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAAt                    >  2:4476439/1‑93 (MQ=255)

                                                                      gTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATc                   >  1:4984040/1‑93 (MQ=255)

                                                                      gTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGCAAATc                   >  2:3988720/1‑93 (MQ=255)

                                                                       ttGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCg                  >  1:286733/1‑93 (MQ=255)

                                                                       ttGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCg                  >  1:3639624/1‑93 (MQ=255)

                                                                        tGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCgg                 <  1:4419695/93‑1 (MQ=255)

                                                                          aCCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCGGAt               <  1:742033/93‑1 (MQ=255)

                                                                           ccGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCGGAtg              >  1:4326471/1‑93 (MQ=255)

                                                                           ccGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCGGAtg              <  2:4133338/93‑1 (MQ=255)

                                                                              cTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCGGATGTGa           >  2:4397028/1‑93 (MQ=255)

                                                                              cTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCGGATGTGa           <  1:2516006/93‑1 (MQ=255)

                                                                              cTCATCCGGATGCTCAGGCTGTAGTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCGGATGTGa           <  2:2960677/93‑1 (MQ=255)

                                                                               tCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCGGATGTGAt          <  1:4131109/93‑1 (MQ=255)

                                                                                cATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCGGATGTGATg         >  2:5189879/1‑93 (MQ=255)

                                                                                cATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCGGATGTGATg         <  2:3070318/93‑1 (MQ=255)

                                                                                 aTCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCGGATGTGATgg        <  2:313066/93‑1 (MQ=255)

                                                                                 aTCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCGGATGTGATgg        >  1:1791240/1‑93 (MQ=255)

                                                                                  tCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCGGATGTGATGgt       >  1:3938705/1‑93 (MQ=255)

                                                                                  tCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCGGATGTGATGgt       <  2:2142780/93‑1 (MQ=255)

                                                                                  tCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCGGATGTGATGgt       >  1:5970303/1‑93 (MQ=255)

                                                                                  tCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCGGATGTGATGgt       >  1:927639/1‑93 (MQ=255)

                                                                                    cGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCGGATGTGATGgtcg     >  2:2227090/1‑93 (MQ=255)

                                                                                      gATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCGGATGTGATGgtcgtc   <  2:5140027/93‑1 (MQ=255)

                                                                                       aTGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCGGATGTGATGGtcgtcg  >  1:5086416/1‑93 (MQ=255)

AGTTTAATGTTCTTGCCAGCCAGCCAGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGTTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCGGATGTGATGGTCGTCG  >  NC_000913/3934436‑3934615

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CAAGTTTAATGTTCTTGCCAGCCAGCCAGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGTTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCGGATGTGATGGTCGTCGGATT  >  NC_000913/3934434‑3934619

CAAGTTTAATGTTCTTGCCAGCCAGCCGGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCT                                                                                              >  SRR4302533.5377689/1‑94 (MQ=60)

CAAGTTTAATGTTCTTGCCAGCCAGCCGGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCT                                                                                              >  SRR4302533.2921827/1‑94 (MQ=60)

CAAGTTTAATGTTCTTGCCAGCCAGCCGGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCT                                                                                              <  SRR4302533.743229/94‑1 (MQ=60)

  AGTTTAATGTTCTTGCCAGCCAGCCGGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCA                                                                                            >  SRR4302533.409594/1‑94 (MQ=60)

   GTTTAATGTTCTTGCCAGCCAGCCGGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAG                                                                                           >  SRR4302533.1492023/1‑94 (MQ=60)

   GTTTAATGTTCTTGCCAGCCAGCCGGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAG                                                                                           <  SRR4302533.3247215/94‑1 (MQ=60)

   GTTTAATGTTCTTGCCAGCCAGCCGGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAG                                                                                           <  SRR4302533.1610934/94‑1 (MQ=60)

    TTTAATGTTCTTGCCAGCCAGCCGGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGG                                                                                          >  SRR4302533.4138131/1‑94 (MQ=60)

      TAATGTTCTTGCCAGCCAGCCGGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCT                                                                                        <  SRR4302533.782407/94‑1 (MQ=60)

      TAATGTTCTTGCCAGCCAGCCGGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCT                                                                                        <  SRR4302533.1699361/94‑1 (MQ=60)

      TAATGTTCTTGCCAGCCAGCCGGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCT                                                                                        >  SRR4302533.2801312/1‑94 (MQ=60)

       AATGTTCTTGCCAGCCAGCCGGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTG                                                                                       <  SRR4302533.1192472/94‑1 (MQ=60)

        ATGTTCTTGCCAGCCAGCCGGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGT                                                                                      <  SRR4302533.1243110/94‑1 (MQ=60)

        ATGTTCTTGCCAGCCAGCCGGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGT                                                                                      >  SRR4302533.670763/1‑94 (MQ=60)

          GTTCTTGCCAGCCAGCCGGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTAT                                                                                    <  SRR4302533.6031807/94‑1 (MQ=60)

           TTCTTGCCAGCCAGCCGGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATT                                                                                   <  SRR4302533.784883/94‑1 (MQ=60)

           TTCTTGCCAGCCAGCCGGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATT                                                                                   <  SRR4302533.1499852/94‑1 (MQ=60)

             CTTGCCAGCCAGCCGGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCG                                                                                 <  SRR4302533.3975469/94‑1 (MQ=60)

             CTTGCCAGCCAGCCGGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCG                                                                                 >  SRR4302533.3472732/1‑94 (MQ=60)

              TTGCCAGCCAGCCGGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGC                                                                                >  SRR4302533.4231956/1‑94 (MQ=60)

                 CCAGCCAGCCGGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCA                                                                             >  SRR4302533.4270727/1‑94 (MQ=60)

                 CCAGCCAGCCGGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCA                                                                             >  SRR4302533.5883605/1‑94 (MQ=60)

                   AGCCAGCCGGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGA                                                                           >  SRR4302533.4868169/1‑94 (MQ=60)

                   AGCCAGCCGGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGA                                                                           >  SRR4302533.6115409/1‑94 (MQ=60)

                    GCCAGCCGGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAA                                                                          <  SRR4302533.6192466/94‑1 (MQ=60)

                     CCAGCCGGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAAT                                                                         >  SRR4302533.5072211/1‑94 (MQ=60)

                     CCAGCCGGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAAT                                                                         >  SRR4302533.2713353/1‑94 (MQ=60)

                     CCAGCCGGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAAT                                                                         <  SRR4302533.2842071/94‑1 (MQ=60)

                     CCAGCCGGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAAT                                                                         >  SRR4302533.515727/1‑94 (MQ=60)

                     CCAGCCGGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAAT                                                                         <  SRR4302533.4996797/94‑1 (MQ=60)

                      CAGCCGGCAGATTTTGATCGCATTAAAGGCTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATG                                                                        >  SRR4302533.5971989/1‑94 (MQ=60)

                       AGCCGGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGA                                                                       >  SRR4302533.4356247/1‑94 (MQ=60)

                        GCCGGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGACTGAT                                                                      >  SRR4302533.2084656/1‑94 (MQ=60)

                        GCCGGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGAT                                                                      >  SRR4302533.3019763/1‑94 (MQ=60)

                        GCCGGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGAT                                                                      <  SRR4302533.1362184/94‑1 (MQ=60)

                         CCGGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATG                                                                     >  SRR4302533.3911488/1‑94 (MQ=60)

                          cggacGATTTTGATCGTATTAAGTGTTTGAACGTAATGCAGAACCGGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGA                                                                    <  SRR4302533.6008159/89‑1 (MQ=60)

                          CGGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGA                                                                    <  SRR4302533.6008160/94‑1 (MQ=60)

                          CGGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGA                                                                    >  SRR4302533.755825/1‑94 (MQ=60)

                          CGGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGA                                                                    >  SRR4302533.5673801/1‑94 (MQ=60)

                          CGGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGA                                                                    >  SRR4302533.5542887/1‑94 (MQ=60)

                           GGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAA                                                                   <  SRR4302533.2164931/94‑1 (MQ=60)

                           GGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAA                                                                   <  SRR4302533.4258434/94‑1 (MQ=60)

                           GGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAA                                                                   >  SRR4302533.1500799/1‑94 (MQ=60)

                            GCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAA                                                                  >  SRR4302533.6140786/1‑94 (MQ=60)

                            GCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAA                                                                  >  SRR4302533.4865509/1‑94 (MQ=60)

                            GCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAA                                                                  <  SRR4302533.5186775/94‑1 (MQ=60)

                            GCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAA                                                                  >  SRR4302533.3600288/1‑94 (MQ=60)

                             CAGATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAAT                                                                 <  SRR4302533.602057/94‑1 (MQ=60)

                              AGATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATG                                                                >  SRR4302533.4035530/1‑94 (MQ=60)

                              AGATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATG                                                                >  SRR4302533.5561403/1‑94 (MQ=60)

                              AGATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATG                                                                <  SRR4302533.4914046/94‑1 (MQ=60)

                               GATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGG                                                               <  SRR4302533.5858826/94‑1 (MQ=60)

                               GATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGG                                                               <  SRR4302533.5328255/94‑1 (MQ=60)

                                ATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGC                                                              <  SRR4302533.4537427/94‑1 (MQ=60)

                                 TTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCG                                                             >  SRR4302533.6300154/1‑94 (MQ=60)

                                  TTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGC                                                            >  SRR4302533.523244/1‑94 (MQ=60)

                                  TTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGC                                                            <  SRR4302533.3262499/94‑1 (MQ=60)

                                   TTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCT                                                           >  SRR4302533.5329291/1‑94 (MQ=60)

                                    TGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTG                                                          >  SRR4302533.4842023/1‑94 (MQ=60)

                                     GATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGG                                                         >  SRR4302533.1197877/1‑94 (MQ=60)

                                     GATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGG                                                         >  SRR4302533.3828730/1‑94 (MQ=60)

                                     GATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGG                                                         <  SRR4302533.4055979/94‑1 (MQ=60)

                                     GATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGG                                                         >  SRR4302533.355788/1‑94 (MQ=60)

                                      ATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGG                                                        <  SRR4302533.2208555/94‑1 (MQ=60)

                                      ATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGG                                                        <  SRR4302533.3610002/94‑1 (MQ=60)

                                      ATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGG                                                        <  SRR4302533.5456377/94‑1 (MQ=60)

                                      ATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGG                                                        >  SRR4302533.4698330/1‑94 (MQ=60)

                                       TCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGC                                                       >  SRR4302533.2978851/1‑94 (MQ=60)

                                       TCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGC                                                       <  SRR4302533.4966005/94‑1 (MQ=60)

                                       TCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGC                                                       <  SRR4302533.5101720/94‑1 (MQ=60)

                                        CGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCG                                                      >  SRR4302533.1075545/1‑94 (MQ=60)

                                         GCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGC                                                     >  SRR4302533.675099/1‑94 (MQ=60)

                                          CATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCG                                                    >  SRR4302533.1856551/1‑94 (MQ=60)

                                           ATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGC                                                   >  SRR4302533.1125445/1‑94 (MQ=60)

                                           ATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGC                                                   >  SRR4302533.2702784/1‑94 (MQ=60)

                                            TTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCT                                                  <  SRR4302533.3577444/94‑1 (MQ=60)

                                            TTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCT                                                  >  SRR4302533.5824979/1‑94 (MQ=60)

                                            TTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCT                                                  >  SRR4302533.3269917/1‑94 (MQ=60)

                                             TAAAGGTTTGAACGTAATGCAGAACCTTTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTG                                                 <  SRR4302533.2488590/94‑1 (MQ=60)

                                             TAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTG                                                 >  SRR4302533.1398232/1‑94 (MQ=60)

                                              AAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGC                                                >  SRR4302533.4025375/1‑94 (MQ=60)

                                              AAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGC                                                <  SRR4302533.533379/94‑1 (MQ=60)

                                               AAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCG                                               >  SRR4302533.5250167/1‑94 (MQ=60)

                                                AGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGC                                              <  SRR4302533.2804519/94‑1 (MQ=60)

                                                AGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGC                                              >  SRR4302533.4832315/1‑94 (MQ=60)

                                                 GGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCG                                             >  SRR4302533.1068962/1‑94 (MQ=60)

                                                  GTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGC                                            >  SRR4302533.4537543/1‑94 (MQ=60)

                                                     TGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACT                                         <  SRR4302533.67976/94‑1 (MQ=60)

                                                      GAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTG                                        <  SRR4302533.4619418/94‑1 (MQ=60)

                                                      GAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTG                                        >  SRR4302533.4982432/1‑94 (MQ=60)

                                                        ACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCA                                      >  SRR4302533.5150030/1‑94 (MQ=60)

                                                         CGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAA                                     >  SRR4302533.1611813/1‑94 (MQ=60)

                                                          GTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAA                                    >  SRR4302533.1600056/1‑94 (MQ=60)

                                                           TAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAAC                                   >  SRR4302533.5399591/1‑94 (MQ=60)

                                                           TAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAAC                                   <  SRR4302533.2008131/94‑1 (MQ=60)

                                                            AATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACT                                  >  SRR4302533.3533272/1‑94 (MQ=60)

                                                            AATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACT                                  <  SRR4302533.5468894/94‑1 (MQ=60)

                                                                CAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCG                              >  SRR4302533.740010/1‑94 (MQ=60)

                                                                CAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCG                              <  SRR4302533.899828/94‑1 (MQ=60)

                                                                 AGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGG                             >  SRR4302533.5486527/1‑94 (MQ=60)

                                                                 AGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGG                             <  SRR4302533.4269542/94‑1 (MQ=60)

                                                                 AGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGG                             >  SRR4302533.5844243/1‑94 (MQ=60)

                                                                  GAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGT                            >  SRR4302533.3066550/1‑94 (MQ=60)

                                                                  GAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGT                            <  SRR4302533.3906944/94‑1 (MQ=60)

                                                                    ACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAA                          <  SRR4302533.4555393/94‑1 (MQ=60)

                                                                     CCTGTTGCCCGCTCATCCGGATGCTCAGGCTGTATTCGCGCCGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCACACTGCCGGTAAA                         >  SRR4302533.5911850/1‑94 (MQ=60)

                                                                     CCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAA                         >  SRR4302533.2703988/1‑94 (MQ=60)

                                                                      CTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAAT                        >  SRR4302533.3300919/1‑94 (MQ=60)

                                                                      CTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAAT                        >  SRR4302533.4725923/1‑94 (MQ=60)

                                                                      CTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAAT                        >  SRR4302533.5688023/1‑94 (MQ=60)

                                                                      CTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAAT                        >  SRR4302533.669419/1‑94 (MQ=60)

                                                                      CTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAAT                        >  SRR4302533.2895638/1‑94 (MQ=60)

                                                                       TGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATC                       >  SRR4302533.5014259/1‑94 (MQ=60)

                                                                       TGTTGACCGCTCATCCGGATGCTCAGCCTGTATTCGCGCAGAATGATGAAATGGCGCGGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATC                       <  SRR4302533.194176/94‑1 (MQ=60)

                                                                        GTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCG                      <  SRR4302533.2387295/94‑1 (MQ=60)

                                                                        GTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCG                      >  SRR4302533.3662003/1‑94 (MQ=60)

                                                                        GTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCG                      >  SRR4302533.288486/1‑94 (MQ=60)

                                                                         TTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCGG                     <  SRR4302533.3239854/94‑1 (MQ=60)

                                                                         TTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCGG                     >  SRR4302533.545001/1‑94 (MQ=60)

                                                                         TTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCGG                     <  SRR4302533.436153/94‑1 (MQ=60)

                                                                          TGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCGGA                    <  SRR4302533.4446417/94‑1 (MQ=60)

                                                                          TGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCGGA                    >  SRR4302533.5300706/1‑94 (MQ=60)

                                                                            ACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCGGATG                  <  SRR4302533.746402/94‑1 (MQ=60)

                                                                            ACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCGGATG                  >  SRR4302533.4352533/1‑94 (MQ=60)

                                                                             CCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCGGATGT                 >  SRR4302533.2548098/1‑94 (MQ=60)

                                                                              CGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCGGATGTG                >  SRR4302533.3384074/1‑94 (MQ=60)

                                                                              CGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCGGATGTG                >  SRR4302533.1973445/1‑94 (MQ=60)

                                                                              CGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCGGATGTG                >  SRR4302533.1711951/1‑94 (MQ=60)

                                                                                CTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCGGATGTGAT              >  SRR4302533.3017505/1‑94 (MQ=60)

                                                                                CTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCGGATGTGAT              <  SRR4302533.2531510/94‑1 (MQ=60)

                                                                                 TCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCGGATGTGATG             <  SRR4302533.4156184/94‑1 (MQ=60)

                                                                                  CATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCGGATGTGATGG            >  SRR4302533.181470/1‑94 (MQ=60)

                                                                                  CATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCGGATGTGATGG            >  SRR4302533.1802132/1‑94 (MQ=60)

                                                                                  CATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCGGATGTGATGG            >  SRR4302533.396617/1‑94 (MQ=60)

                                                                                  CATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCGGATGTGATGG            <  SRR4302533.1709312/94‑1 (MQ=60)

                                                                                  CATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCGGATGTGATGG            <  SRR4302533.1586740/94‑1 (MQ=60)

                                                                                   ATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCGGATGTGATGGT           >  SRR4302533.933085/1‑94 (MQ=60)

                                                                                   ATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCGGATGTGATGGT           >  SRR4302533.6006446/1‑94 (MQ=60)

                                                                                   ATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCGGATGTGATGGT           >  SRR4302533.3962745/1‑94 (MQ=60)

                                                                                      CGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCGGATGTGATGGTCGT        >  SRR4302533.3801717/1‑94 (MQ=60)

                                                                                        GATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCGGATGTGATGGTCGTCG      <  SRR4302533.5984021/94‑1 (MQ=60)

                                                                                        GATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCGGATGTGATGGTCGTCG      >  SRR4302533.5117149/1‑94 (MQ=60)

                                                                                         ATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCGGATGTGATGGTCGTCGG     >  SRR4302533.5459316/1‑94 (MQ=60)

                                                                                         ATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCGGATGTGATGGTCGTCGG     >  SRR4302533.1441464/1‑94 (MQ=60)

                                                                                          TGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCGGATGTGATGGTCGTCGGA    >  SRR4302533.5814639/1‑94 (MQ=60)

                                                                                          TGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCGGATGTGATGGTCGTCGGA    <  SRR4302533.483928/94‑1 (MQ=60)

                                                                                           GCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCGGATGTGATGGTCGTCGGAT   <  SRR4302533.5087618/94‑1 (MQ=60)

                                                                                            CTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCGGATGTGATGGTCGTCGGATT  >  SRR4302533.583209/1‑94 (MQ=60)

                                                                                            CTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCGGATGTGATGGTCGTCGGATT  >  SRR4302533.389177/1‑94 (MQ=60)

                                                                                            CTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCGGATGTGATGGTCGTCGGATT  <  SRR4302533.1327149/94‑1 (MQ=60)

CAAGTTTAATGTTCTTGCCAGCCAGCCAGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGTTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCGGATGTGATGGTCGTCGGATT  >  NC_000913/3934434‑3934619

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`