Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,957,560	+C	intergenic (‑129/‑732)	ppiC ← / → rep	peptidyl‑prolyl cis‑trans isomerase C/rep helicase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,957,558	1	.	C	100.0%	599.4 / NA	183	intergenic (‑127/‑734)	ppiC/rep	peptidyl‑prolyl cis‑trans isomerase C/rep helicase
Reads supporting (aligned to +/- strand): ref base . (0/0); new base C (89/94); total (89/94)

Only 100 of 196 total aligned reads displayed.

AGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑C‑C‑T‑G‑C‑TCGCAGGT‑CCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATG  >  NC_000913/3957471‑3957645

aGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑C‑CGG‑G‑CTTCGCAGGT‑ccc                                                                                      >  1:3455571/1‑92 (MQ=255)

 gCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑C‑CGG‑G‑CTTCGCAGGT‑ccct                                                                                     <  2:4536311/93‑3 (MQ=255)

 gCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑C‑CGG‑G‑CTTCGCAGGT‑cccc                                                                                     >  2:2458756/1‑91 (MQ=255)

   aTGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑C‑CGG‑G‑CTTCGCAGGT‑ccctca                                                                                   >  1:1303296/1‑89 (MQ=255)

     gATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑C‑CGG‑G‑CTTCGCAGGTCCCTCAAg                                                                                  <  1:2409526/93‑1 (MQ=255)

     gATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑C‑CGG‑G‑CTTCGCAGGTCCCTCAAg                                                                                  >  2:2776763/1‑93 (MQ=255)

      aTCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑C‑CGG‑G‑CTTCGCAGGTCCCTCAAgg                                                                                 <  1:4788622/93‑1 (MQ=255)

       tCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑C‑CGG‑G‑CTTCGCAGGTCCCTCAAggg                                                                                <  1:4977672/93‑1 (MQ=255)

       tCCGCACATGCTAACGTAATTGTAAGTCAGGTAGTGGCTGGTACATATATGGCAAACGCCAGGAAAAT‑C‑CGG‑G‑CTTCGCAGGTCCCTCAAggg                                                                                <  2:4478302/93‑1 (MQ=255)

         cGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑C‑CGG‑G‑CTTCGCAGGTCCCTCAAGGGga                                                                              <  2:1555134/93‑1 (MQ=255)

         cGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑C‑CGG‑G‑CTTCGCAGGTCCCTCAAGGGga                                                                              <  1:3250867/93‑1 (MQ=255)

             gATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATTGCAAAAGCCCGGAAAATAC‑G‑G‑G‑CTTCGCAGGTCCCTCAAGGGGAGaaa                                                                          >  1:642179/1‑93 (MQ=255)

             gATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑C‑CGG‑G‑CTTCGCAGGTCCCTCAAGGGGAGaaa                                                                          >  1:3562047/1‑93 (MQ=255)

             gATGCTAACCTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑C‑CGG‑G‑CTTCGCAGGTCCCTCAAGGGGAGaaa                                                                          >  2:3165024/1‑93 (MQ=255)

              aTGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑C‑CGG‑G‑CTTCGCAGGTCCCTCAAGGGGAGaaaa                                                                         >  1:4881539/1‑93 (MQ=255)

              aTGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGGACATATATGGCAAAAGCCCGGAAAAT‑C‑CGG‑G‑CTTCGCAGGTCCCTCAAGGGGAGaaaa                                                                         >  1:5405366/1‑93 (MQ=255)

               tGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑C‑CGG‑G‑CTTCGCAGGTCCCTCAAGGGGAGAAAAc                                                                        >  2:2082728/1‑93 (MQ=255)

               tGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑C‑CGG‑G‑CTTCGCAGGTCCCTCAAGGGGAGAAAAc                                                                        <  1:75657/93‑1 (MQ=255)

                gCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑C‑CGG‑G‑CTTCGCAGGTCCCTCAAGGGGAGAAAACt                                                                       >  1:735500/1‑93 (MQ=255)

                 cTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATTGCAAAAGCCCGGAAAAT‑C‑CGG‑G‑CTTCGCAGGTCCCTCAAGGGGAGAAAACtt                                                                      >  2:4841320/1‑93 (MQ=255)

                  tAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑C‑CGG‑G‑CTTCGCAGGTCCCTCAAGGGGAGGAAAATTa                                                                     >  2:1425346/1‑93 (MQ=255)

                  tAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑C‑CGG‑G‑CTTCGCAGGTCCCTCAAGGGGAGAAAACTTa                                                                     >  1:4514951/1‑93 (MQ=255)

                  tAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑C‑CGG‑G‑CTTCGCAGGTCCCTCAAGGGGAGAAAACTTa                                                                     <  2:1311989/93‑1 (MQ=255)

                   aaCGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑C‑CGG‑G‑CTTCGCAGGTCCCTCCAGGGGAGAAAACTTAg                                                                    >  1:57982/1‑93 (MQ=255)

                   aaCGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑C‑CGG‑G‑CTTCGCAGGTCCCTCAAGGGGAGAAAACTTAg                                                                    <  1:1240149/93‑1 (MQ=255)

                   aaCGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAC‑C‑CGG‑G‑CTTCGCAGGTCCCTCAAGGGGAGAAAACTTAg                                                                    <  2:4653147/93‑1 (MQ=255)

                    aCGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑C‑CGG‑G‑CTTCGCAGGTCCCTCAAGGGGAGAAAACTTAgg                                                                   >  2:4737667/1‑93 (MQ=255)

                     cGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑C‑CGG‑G‑CTTCGCAGGTCCCTCAAGGGGAGAAAACTTAggg                                                                  >  1:6164108/1‑93 (MQ=255)

                         aTTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑C‑CGG‑G‑CTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCctc                                                              <  1:3856960/93‑1 (MQ=255)

                          ttGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑C‑CGG‑G‑CTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCctct                                                             >  2:3576236/1‑93 (MQ=255)

                           tGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑C‑CGG‑G‑CTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTa                                                            <  1:6038221/93‑1 (MQ=255)

                           tGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑C‑C‑GGG‑CTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTa                                                            <  1:157754/93‑1 (MQ=255)

                              cagtcagGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑C‑CGG‑G‑CTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATgg                                                         <  1:144459/93‑1 (MQ=255)

                                gtcagGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑C‑CGG‑G‑CTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGc                                                       <  2:258224/93‑1 (MQ=255)

                                   agGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑C‑CGG‑G‑CTTCGCAGGTCCCTCAAGGGGAGAAAACTTATGGGCTCTATGGGCTAc                                                    >  2:5323197/1‑93 (MQ=255)

                                   agGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑C‑CGG‑G‑CTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTAc                                                    >  2:300605/1‑93 (MQ=255)

                                   agGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑C‑CGG‑G‑CTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTAc                                                    >  1:1022326/1‑93 (MQ=255)

                                   agGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑C‑C‑GGG‑CTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTAc                                                    >  1:4381899/1‑93 (MQ=255)

                                    gggAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑C‑C‑GGG‑CTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACa                                                   >  1:2867184/1‑93 (MQ=255)

                                     ggAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑C‑CGG‑G‑CTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACaa                                                  >  2:2185812/1‑93 (MQ=255)

                                      gAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑C‑CGG‑G‑CTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACaaa                                                 >  2:6203017/1‑93 (MQ=255)

                                       aGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑C‑CGG‑G‑CTTCGCAGGTCCCTCAAGGGGAGACAACTTAGGGCCTCTATGGGCTACAAAg                                                >  1:2441810/1‑93 (MQ=255)

                                       aGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑C‑CGG‑G‑CTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAg                                                <  2:3791309/93‑1 (MQ=255)

                                       aGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑C‑CGG‑G‑CTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAg                                                <  2:784676/93‑1 (MQ=255)

                                        gTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑C‑CGG‑G‑CTTCGCAGGTCCCTCAAGGGGAGAAAACCTAGGGCCTCTATGGGCTACAAAGt                                               >  2:3904460/1‑93 (MQ=255)

                                         tGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑C‑CGG‑G‑CTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTc                                              <  1:4641122/93‑1 (MQ=255)

                                          ggCTGGTACATATATGGCAAAAGCCCGGAAAAT‑C‑CGG‑G‑CTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCa                                             >  2:585892/1‑93 (MQ=255)

                                           gCTGGTACATATATGGCAAAAGCCCTGAAAAT‑C‑CGG‑G‑CTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCAt                                            <  2:4185763/93‑1 (MQ=255)

                                           gCTGGTACATATATGGCAAAAGCCCGGAAAAT‑C‑CGG‑G‑CTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCAt                                            >  1:5840035/1‑93 (MQ=255)

                                            cTGGTACATATATGGCAAAAGCCCGGAAAAT‑C‑CGG‑G‑CTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCAtt                                           <  2:4329855/93‑1 (MQ=255)

                                            cTGGTACATATATGGCAAAAGCCCGGAAAAT‑C‑CGG‑G‑CTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCAtt                                           <  1:1915311/93‑1 (MQ=255)

                                            cTGGTACATATATGACAAAAGCCCGGAAAAT‑C‑CGG‑G‑CTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCAtt                                           <  2:2323548/93‑1 (MQ=255)

                                             tGGTACATATATGGCAAAAGCCCGGAAAAT‑C‑CGG‑G‑CTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTa                                          >  2:4068137/1‑93 (MQ=255)

                                              ggTACATATATGGCAAAAGCCCGGAAAAT‑C‑CGG‑G‑CTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTAt                                         >  1:3163164/1‑93 (MQ=255)

                                              ggTACATATATGGCAAAAGCCCGGAAAAT‑C‑CGG‑G‑CTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTAt                                         <  2:5161539/93‑1 (MQ=255)

                                              ggTACATATATGGCAAAAGCCCGGAAAAT‑C‑CGG‑G‑CTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTAt                                         >  1:5637528/1‑93 (MQ=255)

                                              gctACATATATGGCAAAAGCCCGGAAAAT‑C‑CGG‑G‑CTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTAt                                         <  2:3289574/91‑1 (MQ=255)

                                                tACATATATGGCAAAAGCCCGGAAAAT‑C‑CGG‑G‑CTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCg                                       <  1:1072851/93‑1 (MQ=255)

                                                tACATATATGGCAAAAGCCCGGAAAAT‑C‑CGG‑G‑CTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCg                                       >  1:2084534/1‑93 (MQ=255)

                                                tACATATATGGCAAAAGCCCGGAAAAT‑C‑CGG‑G‑CTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCg                                       <  1:3696578/93‑1 (MQ=255)

                                                 aCATATATGGCAAAAGCCCGGAAAAT‑CGG‑G‑G‑CTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCgg                                      <  1:3516903/93‑1 (MQ=255)

                                                 aCATATATGGCAAAAGCCCGAAAAAT‑C‑CGG‑G‑CTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCgg                                      <  2:107705/93‑1 (MQ=255)

                                                  cATATATGGCAAAAGCCCGGAAAAT‑C‑CGG‑G‑CTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCggg                                     <  2:5922720/93‑1 (MQ=255)

                                                  cATATATGGCAAAAGCCCGGAAAAT‑C‑CGG‑G‑CTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCggg                                     >  1:3787687/1‑93 (MQ=255)

                                                  cATATATGGCAAAAGCCCGGAAAAT‑C‑C‑GGG‑CTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCggg                                     >  1:2183591/1‑93 (MQ=255)

                                                  cATATATGGCAAAAGCCCGGAAAAT‑C‑C‑G‑GTCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCggg                                     <  2:3198378/93‑1 (MQ=255)

                                                   atatatGGCAAAAGCCCGGAAAAT‑C‑CGG‑G‑CTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCgggg                                    <  2:101719/93‑1 (MQ=255)

                                                   atatatGGCAAAAGCCCGGAAAAT‑C‑CGG‑G‑CTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCgggg                                    <  2:3650772/93‑1 (MQ=255)

                                                   atatatGGCAAAAGCCCGGAAAAT‑C‑C‑GGG‑CTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCgggg                                    >  2:2555837/1‑93 (MQ=255)

                                                    tatatGGCAAAAGCCCGGAAAAT‑C‑CGG‑G‑CTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGa                                   >  2:6090647/1‑93 (MQ=255)

                                                    tatatGGCAAAAGCCCGGAAAAT‑C‑CGG‑G‑CTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGa                                   >  2:4433691/1‑93 (MQ=255)

                                                    tatatGGCAAAAGCCCGGAAAAT‑C‑CGG‑G‑CTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGa                                   >  1:3946054/1‑93 (MQ=255)

                                                    tatatGGCAAAAGCCCGGAAAAT‑C‑CGG‑G‑CTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGa                                   >  2:3689507/1‑93 (MQ=255)

                                                     atatGGCAAAAGCCCGGAAAAT‑C‑CGG‑G‑CTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGAt                                  <  2:6142513/93‑1 (MQ=255)

                                                         ggCAAAAGCCCGGAAAAT‑C‑CGG‑G‑CTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGtt                              >  2:2901976/1‑93 (MQ=255)

                                                          gCAAAAGCCCGGAAAAT‑C‑CGG‑G‑CTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGtta                             <  1:5123838/93‑1 (MQ=255)

                                                          gCAAAAGCCCGGAAAAT‑C‑CGG‑G‑CTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAATCATTATCGGCGATGGtta                             <  2:6162393/93‑1 (MQ=255)

                                                           cAAAAGCCCGGAAAAT‑C‑CGG‑G‑CTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGttat                            <  1:6063093/93‑1 (MQ=255)

                                                           cAAAAGCCCGGAAAAT‑C‑C‑GGG‑CTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGttat                            >  1:3033190/1‑93 (MQ=255)

                                                           cAAAAGCCCGGAAAAT‑C‑C‑GGG‑CTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGttat                            >  2:4412451/1‑93 (MQ=255)

                                                            aaaaGCCCGGAAAAT‑C‑CGG‑G‑CTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGttatt                           >  1:3339664/1‑93 (MQ=255)

                                                            aaaaGCCCGGAAAAT‑C‑CGG‑G‑CTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGttatt                           >  2:3235026/1‑93 (MQ=255)

                                                             acaGCCCGGAAAAT‑C‑CGG‑G‑CTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGttattt                          <  1:3193735/91‑1 (MQ=255)

                                                             aaaGCCCGGAAAAT‑C‑CGG‑G‑CTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGttattt                          <  1:4376920/93‑1 (MQ=255)

                                                               aGCCCGGAAAAT‑C‑CGG‑G‑CTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGttatttta                        <  2:3096995/93‑1 (MQ=255)

                                                                gCCCGGAAAAT‑C‑CGG‑G‑CTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGttattttat                       >  2:5723997/1‑93 (MQ=255)

                                                                gCCCGGAAAAT‑C‑CGG‑G‑CTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGttattttat                       <  1:1986737/93‑1 (MQ=255)

                                                                  ccGGAAAAT‑C‑CGG‑G‑CTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTg                     >  2:4676026/1‑93 (MQ=255)

                                                                  ccGGAAAAT‑C‑CGG‑G‑CTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTg                     <  2:567894/93‑1 (MQ=255)

                                                                  ccGGAAAAT‑C‑CGG‑G‑CTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTg                     >  1:888138/1‑93 (MQ=255)

                                                                   cGGAAAAT‑C‑CGG‑G‑CTTCGCAGGTCCCTCACGGGGAGAAAGCTTAGGGGCTGTATGGGCTACAAACTCATTATCGGGGATGGTCATTTTCTTGt                    >  2:1746127/1‑93 (MQ=255)

                                                                                  ggcttCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAg         <  2:3442087/89‑1 (MQ=255)

                                                                                   gcttCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGc        <  1:2606831/90‑1 (MQ=255)

                                                                                    cttCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCg       >  1:5544678/3‑93 (MQ=255)

                                                                                        gCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGatat   >  1:1741517/1‑93 (MQ=255)

                                                                                        gCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGatat   <  1:3760503/93‑1 (MQ=255)

                                                                                        gCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGatat   <  1:5306011/93‑1 (MQ=255)

                                                                                         cAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATg  <  1:4169615/93‑1 (MQ=255)

                                                                                         cAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATg  >  2:3484087/1‑93 (MQ=255)

                                                                                         cAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATg  >  1:1495779/1‑93 (MQ=255)

AGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑C‑C‑T‑G‑C‑TCGCAGGT‑CCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATG  >  NC_000913/3957471‑3957645

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATC‑‑‑‑CTG‑‑CTCGCAGGT‑CCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTG  >  NC_000913/3957478‑3957651

TCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATC‑‑‑‑CGGGCTTCGCAGGTCCCTCAAGGGG                                                                                     <  SRR4302533.5007857/94‑1 (MQ=60)

  CGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATC‑‑‑‑CGGGCTTCGCAGGTCCCTCAAGGGGAG                                                                                   <  SRR4302533.3270915/94‑1 (MQ=60)

    CAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATC‑‑‑‑CGGGCTTCGCAGGTCCCTCAAGGGGAGAA                                                                                 <  SRR4302533.321457/94‑1 (MQ=60)

     AGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATTGCAAAAGCCCGGAAAATA‑‑‑‑CGGGCTTCGCAGGTCCCTCAAGGGGAGAAA                                                                                >  SRR4302533.645954/1‑94 (MQ=60)

     AGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATC‑‑‑‑CGGGCTTCGCAGGTCCCTCAAGGGGAGAAA                                                                                >  SRR4302533.3583993/1‑94 (MQ=60)

     AGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATC‑‑‑‑CGGGCTTCGCAGGTCCCTCAAGGGGAGAAA                                                                                >  SRR4302533.2765271/1‑94 (MQ=60)

     AGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCACGGACAATC‑‑‑‑CGGGCTTCGCAGGTCCCTCAAGGGGAGAAA                                                                                >  SRR4302533.3120191/1‑94 (MQ=60)

      GATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATC‑‑‑‑CGGGCTTCGCAGGTCCCTCAAGGGGAGAAAA                                                                               >  SRR4302533.4911113/1‑94 (MQ=60)

      GATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATC‑‑‑‑CGGGCTTCGCAGGTCCCTCAAGGGGAGAAAA                                                                               >  SRR4302533.3487808/1‑94 (MQ=60)

      GATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGGACATATATGGCAAAAGCCCGGAAAATC‑‑‑‑CGGGCTTCGCAGGTCCCTCAAGGGGAGAAAA                                                                               >  SRR4302533.5437849/1‑94 (MQ=60)

        TGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATC‑‑‑‑CGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACT                                                                             <  SRR4302533.76149/94‑1 (MQ=60)

        TGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATC‑‑‑‑CGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACT                                                                             >  SRR4302533.739827/1‑94 (MQ=60)

        TGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATC‑‑‑‑CGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACT                                                                             <  SRR4302533.1922281/94‑1 (MQ=60)

         GCTANCGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATC‑‑‑‑CGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTT                                                                            <  SRR4302533.2110872/94‑1 (MQ=60)

          CTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATC‑‑‑‑CGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTA                                                                           >  SRR4302533.4542290/1‑94 (MQ=60)

           TAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATTTGGCAAAAACCCGGAAAATC‑‑‑‑CGGGCTTCGCAGGTGCCTCAAGGGGAGAAAACTTAG                                                                          >  SRR4302533.2611431/1‑94 (MQ=60)

           TAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATC‑‑‑‑CGGGCTTCGCAGGTCCCTCCAGGGGAGAAAACTTAG                                                                          >  SRR4302533.58365/1‑94 (MQ=60)

            AACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATC‑‑‑‑CGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGG                                                                         <  SRR4302533.1247558/94‑1 (MQ=60)

             ACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATC‑‑‑‑CGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGG                                                                        <  SRR4302533.1186244/94‑1 (MQ=60)

             ACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATC‑‑‑‑CGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGG                                                                        <  SRR4302533.738655/94‑1 (MQ=60)

             ACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATC‑‑‑‑CGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGG                                                                        >  SRR4302533.6201165/1‑94 (MQ=60)

               GTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATC‑‑‑‑CGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCC                                                                      >  SRR4302533.1608584/1‑94 (MQ=60)

                  ATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATC‑‑‑‑CGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCT                                                                   <  SRR4302533.3880509/94‑1 (MQ=60)

                    TGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATC‑‑‑‑CGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTAT                                                                 <  SRR4302533.6074810/94‑1 (MQ=60)

                    TGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATC‑‑‑‑CGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTAT                                                                 <  SRR4302533.158760/94‑1 (MQ=60)

                       CAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATC‑‑‑‑CGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGG                                                              <  SRR4302533.754541/94‑1 (MQ=60)

                       CAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATC‑‑‑‑CGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGG                                                              <  SRR4302533.145383/94‑1 (MQ=60)

                       CAGTCAGGGAGTGGCTGGTACATATAGGGCAAAAGCCCGGAAAATC‑‑‑‑CGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGG                                                              <  SRR4302533.48747/94‑1 (MQ=60)

                         GTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATC‑‑‑‑CGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCT                                                            >  SRR4302533.2990345/1‑94 (MQ=60)

                          TCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATC‑‑‑‑CGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTA                                                           <  SRR4302533.5626963/94‑1 (MQ=60)

                          TCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATC‑‑‑‑CGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTA                                                           >  SRR4302533.154208/1‑94 (MQ=60)

                           CAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATC‑‑‑‑CGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTAC                                                          >  SRR4302533.4408356/1‑94 (MQ=60)

                           CAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATC‑‑‑‑CGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTAC                                                          >  SRR4302533.1028336/1‑94 (MQ=60)

                           CAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATC‑‑‑‑CGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTAC                                                          >  SRR4302533.2101805/1‑94 (MQ=60)

                            AGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATC‑‑‑‑CGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACA                                                         >  SRR4302533.2884825/1‑94 (MQ=60)

                             GGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATC‑‑‑‑CGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAA                                                        >  SRR4302533.1499288/1‑94 (MQ=60)

                               GAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATC‑‑‑‑CGGGCTTCGCAGGTCCCTCAAGGGGAGACAACTTAGGGCCTCTATGGGCTACAAAG                                                      >  SRR4302533.2456884/1‑94 (MQ=60)

                                 GTGGCTGGTACATATATGGCAAAAGCCCGGAAAAAC‑‑‑‑CGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTC                                                    >  SRR4302533.1437600/1‑94 (MQ=60)

                                  TGGCTGGTACATATATGGCAAAAGCCCGGAAAATC‑‑‑‑CGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCA                                                   <  SRR4302533.4669242/94‑1 (MQ=60)

                                   GGCTGGTACATATATGGCAAAAGCCCGGAAAATC‑‑‑‑CGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCAT                                                  >  SRR4302533.5875346/1‑94 (MQ=60)

                                   GGCTGGTACATATATGACAAAAGCCGGGAAAATC‑‑‑‑CGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCAT                                                  <  SRR4302533.4407913/94‑1 (MQ=60)

                                    GCTGGTACATATATGGCAAAAGCCCGGAAAATC‑‑‑‑CGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATT                                                 >  SRR4302533.3911267/1‑94 (MQ=60)

                                     CTGGTACATATATGGCAAAAGCCCGGAAAATC‑‑‑‑CGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTA                                                <  SRR4302533.1927004/94‑1 (MQ=60)

                                     CTGGTACATATATGGCAAAAGCCCGGAAAATC‑‑‑‑CGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTA                                                >  SRR4302533.6212914/1‑94 (MQ=60)

                                      TGGTACATATATGGCAAAAGCCCGGAAAATC‑‑‑‑CGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTAT                                               >  SRR4302533.3182634/1‑94 (MQ=60)

                                      TGGTACATATATGGCAAAAGCCCGGAAAATC‑‑‑‑CGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTAT                                               >  SRR4302533.5326027/1‑94 (MQ=60)

                                      TGGTACATATATGGCAAAAGCCCGGAAAATC‑‑‑‑CGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTAT                                               >  SRR4302533.1991407/1‑94 (MQ=60)

                                      TGGTACATATATGGCAAAAGCCCGGAAAATC‑‑‑‑CGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTAT                                               >  SRR4302533.5671616/1‑94 (MQ=60)

                                      TGGTACATATATGGCAAAAGCCAGGAAAATC‑‑‑‑CGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTAT                                               <  SRR4302533.3575708/94‑1 (MQ=60)

                                       GGTACATATATGGCAAAAGCCCGGAAAATC‑‑‑‑CGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATC                                              >  SRR4302533.4768528/1‑94 (MQ=60)

                                       GGTACATATATGGCAAAAGCCCGGAAAATC‑‑‑‑CGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATC                                              <  SRR4302533.2304038/94‑1 (MQ=60)

                                        GTACATATATGGCAAAAGCCCGGAAAATC‑‑‑‑CGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCG                                             >  SRR4302533.2097368/1‑94 (MQ=60)

                                         TACATATATGGCAAAAGCCCGGAAAATC‑‑‑‑CGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGG                                            <  SRR4302533.3719285/94‑1 (MQ=60)

                                         TACATATATGGCAAAAGCCCGGAAAATC‑‑‑‑CGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGG                                            <  SRR4302533.1079176/94‑1 (MQ=60)

                                          ACATATATGGCAAAAGCCCGGAAAATCGGGGCT‑‑‑‑TCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGG                                           <  SRR4302533.3538585/94‑1 (MQ=60)

                                          ACATATATGGCAAAAGCCCGGAAAATC‑‑‑‑CGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGG                                           >  SRR4302533.2197055/1‑94 (MQ=60)

                                          ACATATATGGCAAAAGCCCGGAAAATC‑‑‑‑CGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGG                                           >  SRR4302533.3810880/1‑94 (MQ=60)

                                           CATATATGGCAAAAGCCCGGAAAATC‑‑‑‑CGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGG                                          <  SRR4302533.336048/94‑1 (MQ=60)

                                           CATATATGGCAAAAGCCCGGAAAATC‑‑‑‑CGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGG                                          <  SRR4302533.5873103/94‑1 (MQ=60)

                                           CATATATGGCAAAAGCCCGGAAAATC‑‑‑‑CGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGG                                          <  SRR4302533.5151730/94‑1 (MQ=60)

                                            ATATATGGCAAAAGCCCGGAAAATC‑‑‑‑CGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGA                                         >  SRR4302533.3970120/1‑94 (MQ=60)

                                             TATATGGCAAAAGCCCGGAAAATC‑‑‑‑CGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGAT                                        >  SRR4302533.4065069/1‑94 (MQ=60)

                                              ATATGGCAAAAGCCCGGAAAATC‑‑‑‑CGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATG                                       >  SRR4302533.1265067/1‑94 (MQ=60)

                                              ATATGGCAAAAGCCCGGAAAATC‑‑‑‑CGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATG                                       <  SRR4302533.4648385/94‑1 (MQ=60)

                                               TATGGCAAAAGCCCGGAAAATC‑‑‑‑CGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGG                                      <  SRR4302533.5481420/94‑1 (MQ=60)

                                               TATGGCAAAAGCCCGGAAAATC‑‑‑‑CGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGG                                      <  SRR4302533.3842755/94‑1 (MQ=60)

                                                ATGGCAAAAGCCCGGAAAATC‑‑‑‑CGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGT                                     >  SRR4302533.420815/1‑94 (MQ=60)

                                                ATGGCAAAAGCCCGGAAAATC‑‑‑‑CGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGT                                     >  SRR4302533.3183258/1‑94 (MQ=60)

                                                 TGGCAAAAGCCCGGAAAATC‑‑‑‑CGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTT                                    >  SRR4302533.6188781/1‑94 (MQ=60)

                                                   GCAAAAGCCCGGAAAATC‑‑‑‑CGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTAT                                  <  SRR4302533.5154793/94‑1 (MQ=60)

                                                   GCAAAAGCCCGGAAAATC‑‑‑‑CGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTAT                                  >  SRR4302533.3051745/1‑94 (MQ=60)

                                                    CAAAAGCCCGGAAAATC‑‑‑‑CGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATT                                 >  SRR4302533.3360304/1‑94 (MQ=60)

                                                    CAAAAGCCCGGAAAATC‑‑‑‑CGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATT                                 <  SRR4302533.6099797/94‑1 (MQ=60)

                                                     tggaaaaaacccgaaaattccggactTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTT                                    <  SRR4302533.3294857/68‑1 (MQ=60)

                                                      ACAGCCCGGAAAATC‑‑‑‑CGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTT                               <  SRR4302533.3213383/94‑1 (MQ=60)

                                                      AAAGCCCGGAAAATC‑‑‑‑CGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTT                               <  SRR4302533.6045558/94‑1 (MQ=60)

                                                      AAAGCCCGGAAAATC‑‑‑‑CGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTT                               <  SRR4302533.4403342/94‑1 (MQ=60)

                                                        AGCCCGGAAAATC‑‑‑‑CGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTAT                             <  SRR4302533.2095547/94‑1 (MQ=60)

                                                         GCCCGGAAAATC‑‑‑‑CGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATT                            <  SRR4302533.1998891/94‑1 (MQ=60)

                                                          CCCGGAAAATC‑‑‑‑CGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTG                           >  SRR4302533.893352/1‑94 (MQ=60)

                                                           CCGGAAAATC‑‑‑‑CGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGT                          >  SRR4302533.1833644/1‑94 (MQ=60)

                                                           CCGGAAAATC‑‑‑‑CGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGT                          <  SRR4302533.607732/94‑1 (MQ=60)

                                                                 ggaaaatccgggctTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCA                        >  SRR4302533.6160291/15‑94 (MQ=60)

                                                                 ggaaaatccgggctTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGCGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCA                        <  SRR4302533.4030930/80‑1 (MQ=60)

                                                                  gaaaatccgggctTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAT                       >  SRR4302533.5187365/14‑94 (MQ=60)

                                                                    aaatccgggctTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATT                     >  SRR4302533.3655791/12‑94 (MQ=60)

                                                                    aaatccgggctTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATT                     >  SRR4302533.5616523/12‑94 (MQ=60)

                                                                    aaatccgggctTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATT                     <  SRR4302533.1685368/83‑1 (MQ=60)

                                                                    aaatccgggctTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATT                     <  SRR4302533.2175312/83‑1 (MQ=60)

                                                                    aaatccgggctTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATT                     >  SRR4302533.1221104/12‑94 (MQ=60)

                                                                     aatccgggctTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTT                    <  SRR4302533.5634568/84‑1 (MQ=60)

                                                                     aatccgggctTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTT                    >  SRR4302533.3251326/11‑94 (MQ=60)

                                                                     aatccgggctTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTT                    <  SRR4302533.4774735/84‑1 (MQ=60)

                                                                      atccgggctTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTA                   <  SRR4302533.4048130/85‑1 (MQ=60)

                                                                      atccgggctTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTA                   <  SRR4302533.1493207/85‑1 (MQ=60)

                                                                        ccgggctTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATG                 >  SRR4302533.5863101/8‑94 (MQ=60)

                                                                        ccgggctTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATG                 <  SRR4302533.499048/87‑1 (MQ=60)

                                                                         cgggctTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGA                <  SRR4302533.3184501/88‑1 (MQ=60)

                                                                          gggctTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAG               <  SRR4302533.5559008/89‑1 (MQ=60)

                                                                          gggctTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAG               <  SRR4302533.5383798/89‑1 (MQ=60)

                                                                           ggctTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGC              <  SRR4302533.2518480/90‑1 (MQ=60)

                                                                           ggctTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGC              <  SRR4302533.2456835/90‑1 (MQ=60)

                                                                           ggctTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGC              <  SRR4302533.4422545/90‑1 (MQ=60)

                                                                            gctTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCG             >  SRR4302533.5578168/4‑94 (MQ=60)

                                                                            gctTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCG             <  SRR4302533.2622841/91‑1 (MQ=60)

                                                                            gctTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCG             <  SRR4302533.3328556/91‑1 (MQ=60)

                                                                              TTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGAT           >  SRR4302533.4997133/1‑94 (MQ=60)

                                                                              TTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGAT           >  SRR4302533.578798/1‑94 (MQ=60)

                                                                                CGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATAT         >  SRR4302533.1752078/1‑94 (MQ=60)

                                                                                 GCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATG        <  SRR4302533.3783552/94‑1 (MQ=60)

                                                                                 GCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATG        <  SRR4302533.5337809/94‑1 (MQ=60)

                                                                                 GCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATG        >  SRR4302533.1504792/1‑94 (MQ=60)

                                                                                  CAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGG       <  SRR4302533.4194882/94‑1 (MQ=60)

                                                                                   AGGTCCCTCAATGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGC      <  SRR4302533.5349773/94‑1 (MQ=60)

                                                                                   AGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGC      <  SRR4302533.968263/94‑1 (MQ=60)

                                                                                   AGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGC      >  SRR4302533.4233844/1‑94 (MQ=60)

                                                                                     gtcCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCAT    >  SRR4302533.4518824/4‑94 (MQ=60)

                                                                                      tcCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATT   >  SRR4302533.6292227/3‑94 (MQ=60)

                                                                                      tcCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATT   >  SRR4302533.1963059/3‑94 (MQ=60)

                                                                                      tcCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATT   >  SRR4302533.572002/3‑94 (MQ=60)

                                                                                      C‑CCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTG  <  SRR4302533.2724123/94‑1 (MQ=60)

TCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATC‑‑‑‑CTG‑‑CTCGCAGGT‑CCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTG  >  NC_000913/3957478‑3957651

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 7 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`