Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,490,650 C→T L90L (CTG→CTA)  idnO ← 5‑keto‑D‑gluconate 5‑reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,490,6500CT100.0% 899.6 / NA 240L90L (CTG→CTA) idnO5‑keto‑D‑gluconate 5‑reductase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (119/121);  total (119/121)

Only 100 of 243 total aligned reads displayed.

GGCGGTCTGGTTTACTGCGATCACATCATTCCACTCTTGTTCAGGGAACTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACCAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAACGGCGGCATCAATTTCATGTTTATGAGTAACATTAAAAGGTGCGGCAAC  >  NC_000913/4490560‑4490739
                                                                                          |                                                                                         
ggcggTCTGGTTTACTGCGATCACATCATTCCACTCTTGTTCAGGGAACTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAg                                                                                         <  1:3226536/93‑1 (MQ=255)
ggcggTCTGGTTTACTGCGATCACATCATTCCACTCTTGTTCAGGGAACTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAg                                                                                         <  2:4473738/93‑1 (MQ=255)
ggcgaTCTGGTTTACTGCGATCACATCATTCCACTCTTGTTCAGGGATCTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAg                                                                                         <  2:323929/93‑1 (MQ=255)
   ggTCTGGTTTACTGCGATCACATCATTCCACTCTTGTTCAGGGAACTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGcac                                                                                      <  1:276973/93‑1 (MQ=255)
    gTCTGGTTTACTGCGATCACATCATTCCACTCTTGTTCAGGGAACTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGcaca                                                                                     <  2:2788235/93‑1 (MQ=255)
    gTCTGGTTTACTGCGATCACATCATTCCACTCTTGTTCAGGGAACTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGcaca                                                                                     >  2:5866840/1‑93 (MQ=255)
     tCTGGTTTACTGCGATCACATCATTCCACTCTTGTTCAGGGAACTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACAt                                                                                    >  1:2775234/1‑93 (MQ=255)
     tCTGGTTTACTGCGATCACATCATTCCACTCTTGTTCAGGGAACTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACAt                                                                                    >  2:2397182/1‑93 (MQ=255)
     tCTGGTTTACTGCGATCACATCATTCCACTCTTGTTCAGGGAACTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACAt                                                                                    >  1:212256/1‑93 (MQ=255)
     tCTGGTTTACTGCGATCACATCATTCCACTCTTGTTCAGGGAACTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACAt                                                                                    >  1:2449390/1‑93 (MQ=255)
     gcTGGTTTACTGCGATCACATCATTCCACTCTTGTTCAGGGAACTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACAt                                                                                    <  1:1685462/92‑1 (MQ=255)
      cTGGTTTACTGCGATCACATCATTCCACTCTTGTTCAGGGAACTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATc                                                                                   <  2:633749/93‑1 (MQ=255)
        ggTTTACTGCGATCACATCATTCCACTCTTGTTCAGGGAACTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCaa                                                                                 <  2:1045475/93‑1 (MQ=255)
        ggTTTACTGCGATCACATCATTCCACTCTTGTTCAGGGAACTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCaa                                                                                 >  2:1007095/1‑93 (MQ=255)
        ggTTTACTGCGATCACATCATTCCACTCTTGTTCAGGGAACTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCaa                                                                                 <  1:124825/93‑1 (MQ=255)
         gTTTACTGCGATCACATCATTCCACTCTTGTTCAGGGAACTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAAt                                                                                <  2:5663384/93‑1 (MQ=255)
          tttACTGCGATCACATCATTCCACTCTTGTTCAGGGAACTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAATg                                                                               <  2:265575/93‑1 (MQ=255)
          tttACTGCGATCACATCATTCCACTCTTGTTCAGGGAACTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAATg                                                                               <  1:3268998/93‑1 (MQ=255)
           ttACTGCGATCACAGCATTCCACTCTTGTTCAGGGAACTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAATgg                                                                              <  1:2987250/93‑1 (MQ=255)
            tACTGCGATCACATCATTCCACTCTTGTTCAGGGAACTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAATggg                                                                             >  2:4276200/1‑93 (MQ=255)
              cTGCGATCACATCATTCCACTCTTGTTCAGGGAACTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAATGGGGc                                                                           <  1:2157370/93‑1 (MQ=255)
              cTGCGATCACATCATTCCACTCTTGTTCAGGGAACTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAATGGGGc                                                                           <  2:2536059/93‑1 (MQ=255)
                 cGATCACATCATTCCACTCTTGTTCAGGGAACTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAATGGGGCCGa                                                                        <  2:4170485/93‑1 (MQ=255)
                 cGATCACATCATTCCACTCTTGTTCAGGGAACTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAATGGGGCCGa                                                                        <  1:443676/93‑1 (MQ=255)
                  gATCACATCATTCCACTCTTGTTCAGGGAACTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAATGGGGCCGAt                                                                       >  1:4542001/1‑93 (MQ=255)
                  gATCACATCATTCCACTCTTGTTCAGGGAACTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAATGGGGCCGAt                                                                       >  2:6021262/1‑93 (MQ=255)
                   aTCACATCATTCCACTCTTGTTCAGGGAACTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAATGGGGCCGATg                                                                      >  2:79544/1‑93 (MQ=255)
                     cacaTCATTCCACTCTTGTTCAGGGAACTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAATGGGGCCGATGTc                                                                    >  1:3763564/1‑93 (MQ=255)
                      acaTCATTCCACTCTTGTTCAGGGAACTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAATGGGGCCGATGTcc                                                                   >  1:563392/1‑93 (MQ=255)
                       catcatTCCACTCTTGTTCAGGGAACTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAATGGGGCCGATGTCCt                                                                  >  2:3664693/1‑93 (MQ=255)
                       catcatTCAACTCTTGTTCAGGGAACTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAATGGGGCCGATGTCCt                                                                  >  2:4961614/1‑93 (MQ=255)
                        atcatTCCACTCTTGTTCAGGGAACTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAATGTGGCCGATGTCCtt                                                                 >  2:1742783/1‑93 (MQ=255)
                          catTCCACTCTTGTTCAGGGAACTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAATGGGGCCGATGTCCtttt                                                               <  2:5216780/93‑1 (MQ=255)
                           atTCCACTCTTGTTCAGGGAACTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTc                                                              >  1:1174261/1‑93 (MQ=255)
                              ccACTCTTGTTCAGGGAACTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGat                                                           <  1:1023880/93‑1 (MQ=255)
                               cACTCTTGTTCAGGGAACTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGata                                                          <  2:4644029/93‑1 (MQ=255)
                               cACTCTTGTTCAGGGAACTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGata                                                          <  2:3598565/93‑1 (MQ=255)
                                aCTCTTGTTCAGGGAACTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGatat                                                         >  1:5209093/1‑93 (MQ=255)
                                  tctTGTTCAGGGCACTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGt                                                       >  2:5932258/1‑93 (MQ=255)
                                    ttGTTCAGGGAACTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTc                                                     <  2:2297065/93‑1 (MQ=255)
                                     tGTTCAGGGAACTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCa                                                    <  1:4097204/93‑1 (MQ=255)
                                      gTTCAGGGAACTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCaa                                                   <  1:5429901/93‑1 (MQ=255)
                                        tCAGGGAACTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAAcg                                                 >  2:3062339/1‑93 (MQ=255)
                                        tCAGGGAACTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAAcg                                                 >  1:397068/1‑93 (MQ=255)
                                        tCAGGGAACTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAAcg                                                 <  1:2514240/93‑1 (MQ=255)
                                          aGGGAACTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAAcggc                                               <  1:5604066/93‑1 (MQ=255)
                                          aGGGAACTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAAcggc                                               >  1:2355835/1‑93 (MQ=255)
                                          aGGGAACTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAAcggc                                               <  2:5546071/93‑1 (MQ=255)
                                          aGGGAACTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAAcggc                                               <  1:5045673/93‑1 (MQ=255)
                                           gggAACTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAAcggcg                                              >  1:4270994/1‑93 (MQ=255)
                                           gggAACTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAAcggcg                                              <  1:4819713/93‑1 (MQ=255)
                                           gggAACTCAGTAAAAGGATGACGGCGCTGGATAACGGCGTTATTCACTAGCACATCAATGGAGCCGATGTCCTTTTCGATATGTTCAAcggcg                                              >  1:4033690/1‑93 (MQ=255)
                                               aCTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAACGGCGGCAt                                          >  2:1450542/1‑93 (MQ=255)
                                                 tCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAACGGCGGCATCa                                        >  2:6249699/1‑93 (MQ=255)
                                                 tCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAATGGAGCCGATGTCCTTTTCGATATGTTCAACGGCGGCATcc                                        >  2:2379959/1‑92 (MQ=255)
                                                  cAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAACGGCGGCATCaa                                       >  2:3265127/1‑93 (MQ=255)
                                                  cAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAACGGCGGCATCaa                                       >  2:4519411/1‑93 (MQ=255)
                                                   aGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAACGGCGGCATCAAt                                      >  1:5097214/1‑93 (MQ=255)
                                                   aGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAACGGCGGCATCAAt                                      >  2:3905493/1‑93 (MQ=255)
                                                   aGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAACGGCGGCATCAAt                                      <  1:6305398/93‑1 (MQ=255)
                                                      aaaaGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAACGGCGGCATCAATTTc                                   >  1:5296730/1‑93 (MQ=255)
                                                      aaaaGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAACGGCGGCATCAATTTc                                   >  1:856662/1‑93 (MQ=255)
                                                       aaaGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAACGGCGGCATCAATTTCa                                  <  1:716737/93‑1 (MQ=255)
                                                       aaaGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAACGGCGGCATCAATTTCa                                  >  1:1605258/1‑93 (MQ=255)
                                                       aaaGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAACGGCGGCATCAATTTCa                                  <  2:5166961/93‑1 (MQ=255)
                                                        aaGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAACGGCGGCATCAATTTCAt                                 <  1:1268044/93‑1 (MQ=255)
                                                         aGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAACGGCGGCATCAATTTCATg                                >  2:1908522/1‑93 (MQ=255)
                                                           gATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAACGGCGGCATCAATTTCATGtt                              >  2:3756553/1‑93 (MQ=255)
                                                           gATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAACGGCGGCATCAATTTCATGtt                              <  1:5878103/93‑1 (MQ=255)
                                                             tGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAACGGCGGCATCAATTTCATGTTTa                            <  2:5436839/93‑1 (MQ=255)
                                                                cGGCGCTGGATACCGGCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAACGGCGGCATCAATTTCATGTTTATGa                         <  2:789516/93‑1 (MQ=255)
                                                                   cgcTGGATACCGGCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAACGGCGGCATCAATTTCATGTTTATGAGTa                      >  2:138674/1‑93 (MQ=255)
                                                                   cgcTGGATACCGGCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAACGGCGGCATCAATTTCATGTTTATGAGTa                      <  1:5839299/93‑1 (MQ=255)
                                                                     cTGGATACCGGCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAACGGCGGCATCAATTTCATGTTTATGAGTAAc                    >  2:1084091/1‑93 (MQ=255)
                                                                      tGGATACCGGCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAACGGCGGCATCAATTTCATGTTTATGAGTAACa                   >  2:6146332/1‑93 (MQ=255)
                                                                      tGGATACCGGCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAACGGCGGCATCAATTTCATGTTTATGAGTAACa                   >  1:2606896/1‑93 (MQ=255)
                                                                       ggATACCGGCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAACGGCGGCATCAATTTCATGTTTATGAGTAACAt                  >  2:4935964/1‑93 (MQ=255)
                                                                        gATACCGGCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAACGGCGGCATCAATTTCATGTTTATGAGTAACAtt                 <  2:1758906/93‑1 (MQ=255)
                                                                        gATACCGCGGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAACGGCGGCATCAATTTCATGTTTATGAGTAACAtt                 <  1:3067624/93‑1 (MQ=255)
                                                                          tACCGGCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAACGGCGGCATCAATTTCATGTTTATGAGTAACATTaa               <  1:573502/93‑1 (MQ=255)
                                                                           aCCGGCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAACGGCGGCATCAATTTCATGTTTATGAGTAACATTaaa              <  1:4669695/93‑1 (MQ=255)
                                                                            ccGGCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAACGGCGGCATCAATTTCATGTTTATGAGTAACATTaaaa             >  2:3949879/1‑93 (MQ=255)
                                                                            ccGGCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAACGGCGGCATCAATTTCATGTTTATGAGTAACATTaaaa             >  1:193499/1‑93 (MQ=255)
                                                                              ggCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAACGGCGGCATCAATTTCATGTTTATGAGTAACATTAAAAgg           <  2:2110994/93‑1 (MQ=255)
                                                                              ggCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAACGGCGGCATCAATTTCATGTTTATGAGTAACATTAAAAgg           >  1:1852077/1‑93 (MQ=255)
                                                                              ggCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAACGGCGGCATCAATTTCATGTTTATGAGTAACATTAAAAgg           <  2:1786501/93‑1 (MQ=255)
                                                                              ggCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAACGGCGGCATCAATTTCATGTTTATGAGTAACATTAAAAgg           <  1:2614113/93‑1 (MQ=255)
                                                                              ggCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAACGGCGGCATCAATTTCATGTTTATGAGTAACATTAAAAgg           <  2:1330276/93‑1 (MQ=255)
                                                                              ggCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAACGGCGGCATCAATTTCATGTTTATGAGTAACATTAAAAgg           <  1:3786030/93‑1 (MQ=255)
                                                                               gCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAACGGCGGCATCAATTTCATGTTTATGAGTAACATTAAAAGGt          <  1:1153304/93‑1 (MQ=255)
                                                                                 gTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAACGGCGGCATCAATTTCATGTTTATGAGTAACATTAAAAGGTGc        >  2:4690368/1‑93 (MQ=255)
                                                                                  ttATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAACGGCGGCATCAATTTCATGTTTATGAGTAACATTAAAAGGTGCg       >  1:3693441/1‑93 (MQ=255)
                                                                                  ttATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAACGGCGGCATCAATTTCATGTTTATGAGTAACATTAAAAGGTGCg       <  2:5113660/93‑1 (MQ=255)
                                                                                  ttATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAACGGCGGCATCAATTTCATGTTTATGAGTAACATTAAAAGGTGCg       >  2:1538775/1‑93 (MQ=255)
                                                                                  ttATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAACGGCGGCATCAATTTCATGTTTATGAGTAACATTAAAAGGTGCg       >  2:1676992/1‑93 (MQ=255)
                                                                                  ttATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAACGGCGGCATCAATTTCATGTTTATGAGTAACATTAAAAGGTGCg       >  2:1855227/1‑93 (MQ=255)
                                                                                   tATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAACGGCGGCATCAATTTCATGTTTATGAGTAACATTAAAAGGTGCgg      >  1:1401823/1‑93 (MQ=255)
                                                                                      tCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAACGGCGGCATCAATTTCATGTTTATGAGTAACATTAAAAGGTGCGGCaa   >  1:4935639/1‑93 (MQ=255)
                                                                                       cACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAACGGCGGCATCAATTTCATGTTTATGAGTAACATTAAAAGGTGCGGCAAc  >  2:4254398/1‑93 (MQ=255)
                                                                                       cACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAACGGCGGCATCAATTTCATGTTTATGAGTAACATTAAAAGGTGCGGCAAc  <  2:2024482/93‑1 (MQ=255)
                                                                                          |                                                                                         
GGCGGTCTGGTTTACTGCGATCACATCATTCCACTCTTGTTCAGGGAACTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACCAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAACGGCGGCATCAATTTCATGTTTATGAGTAACATTAAAAGGTGCGGCAAC  >  NC_000913/4490560‑4490739

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CACGGCGGTCTGGTTTACTGCGATCACATCATTCCACTCTTGTTCAGGGAACTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACCAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAACGGCGGCATCAATTTCATGTTTATGAGTAACATTAAAAGGTGCGGCAACGGCC  >  NC_000913/4490557‑4490743
                                                                                             |                                                                                             
CACGGCGGTCTGGTTTACTGCGATCACATCATTCCACTCTTGTTCAGGGAACTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACT                                                                                               >  SRR4302533.3067496/1‑94 (MQ=60)
CACGGCGGTCTGGTTTACTGCGATCACATCATTCCACTCTTGTTCAGGGAACTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACT                                                                                               >  SRR4302533.6288970/1‑94 (MQ=60)
  CGGCGGTCTGGTTTACTGCGATCACATCATTCCACTCATGTTCAGGGAACTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAG                                                                                             <  SRR4302533.852441/94‑1 (MQ=60)
   GGCGGTCTGGTTTACTGCGATCACATCATTCCACTCTTGTTCAGGGAACTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGC                                                                                            <  SRR4302533.3246429/94‑1 (MQ=60)
   GGCGGTCTGGTTTACTGCGATCACATCATTCCACTCTTGTTCAGGGAACTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGC                                                                                            >  SRR4302533.2124014/1‑94 (MQ=60)
    GCGGTCTGGTTTACTGCGATCACATCATTCCACTCTTGTTCAGGGAACTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCA                                                                                           <  SRR4302533.4548305/94‑1 (MQ=60)
      GGTCTGGTTTACTGCGATCACATCATTCCACTCTTGTTCAGGGAACTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACA                                                                                         >  SRR4302533.700548/1‑94 (MQ=60)
      GGTCTGGTTTACTGCGATCACATCATTCCACTCTTGTTCAGGGAACTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACA                                                                                         >  SRR4302533.779977/1‑94 (MQ=60)
      GGTCTGGTTTACTGCGATCACATCATTCCACTCTTGTTCAGGGAACTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACA                                                                                         <  SRR4302533.278662/94‑1 (MQ=60)
       GTCTGGTTTACTGCGATCACATCATTCCACTCTTGTTCAGGGAACTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACAT                                                                                        >  SRR4302533.2464508/1‑94 (MQ=60)
       GTCTGGTTTACTGCGATCACATCATTCCACTCTTGTTCAGGGAACTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACAT                                                                                        >  SRR4302533.213592/1‑94 (MQ=60)
       GTCTGGTTTACTGCGATCACATCATTCCACTCTTGTTCAGGGAACTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACAT                                                                                        >  SRR4302533.2119152/1‑94 (MQ=60)
       GTCTGGTTTACTGCGATCACATCATTCCACTCTTGTTCAGGGAACTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACAT                                                                                        >  SRR4302533.2792278/1‑94 (MQ=60)
        TCTGGTTTACTGCGATCACATCATTCCACTCTTGTTCAGGGAACTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATC                                                                                       <  SRR4302533.4891039/94‑1 (MQ=60)
        TCTGGTTTACTGCGATCACATCATTCCACTCTTGTTCAGGGAACTCAGTAAAAGGATGACGGCGCCGGATACCGGCGTTATTCACTAGCACATC                                                                                       >  SRR4302533.6096177/1‑94 (MQ=60)
        GCTGGTTTACTGCGATCACATCATTCCACTCTTGTTCAGGGAACTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATC                                                                                       <  SRR4302533.1695674/94‑1 (MQ=60)
         CTGGTTTACTGCGATCACATCATTCCACTCTTGTTCAGGGAACTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCA                                                                                      <  SRR4302533.3881683/94‑1 (MQ=60)
          TGGTTTACTGCGATCACATCATTCCACTCTTGTTCAGGGAACTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAA                                                                                     >  SRR4302533.4661464/1‑94 (MQ=60)
          TGGTTTACTGCGATCACATCATTCCACTCTTGTTCAGGGAACTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAA                                                                                     >  SRR4302533.57219/1‑94 (MQ=60)
           GGTTTACTGCGATCACATCATTCCACTCTTGTTCAGGGAACTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAAT                                                                                    <  SRR4302533.125610/94‑1 (MQ=60)
            GTTTACTGCGATCACATCATTCCACTCTTGTTCAGGGAACTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAATG                                                                                   <  SRR4302533.2464707/94‑1 (MQ=60)
            GTTTACTGCGATCACATCATTCCACTCTTGTTCAGGGAACTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAATG                                                                                   <  SRR4302533.1164357/94‑1 (MQ=60)
            GTTTACTGCGATCACATCATTCCACTCTTGTTCAGGGAACTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAATG                                                                                   <  SRR4302533.3193591/94‑1 (MQ=60)
             TTTACTGCGATCACATCATTCCACTCTTGTTCAGGGAACTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAATGG                                                                                  <  SRR4302533.3289165/94‑1 (MQ=60)
             TTTACTGCGATCACATCATTCCACTCTTGTTCAGGGAACTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAATGG                                                                                  <  SRR4302533.2419695/94‑1 (MQ=60)
              TTACTGCGATCACATCATTCCACTCTTGTTCAGGGAACTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAATGGG                                                                                 <  SRR4302533.3789714/94‑1 (MQ=60)
              TTACTGCGATCACATCATTCCACTCTTGTTCAGGGAACTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAATGGG                                                                                 <  SRR4302533.4062154/94‑1 (MQ=60)
              TTACTGCGATCACAGCATTCCACTCTTGTTCAGGGAACTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAATGGG                                                                                 <  SRR4302533.3005523/94‑1 (MQ=60)
                ACTGCAATCACATCATTCCACTCTTGTTCAGGGAACTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAATGGGGC                                                                               >  SRR4302533.6209537/1‑94 (MQ=60)
                 CTGCGATCACATCATTCCACTCTTGTTCAGGGAACTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAATGGGGCC                                                                              <  SRR4302533.5270331/94‑1 (MQ=60)
                 CTGCGATCACATCATTCCACTCTTGTTCAGGGAACTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAATGGGGCC                                                                              <  SRR4302533.2170651/94‑1 (MQ=60)
                  TGCGATCACATCATTCCACTCTTGTTCAGGGAACTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAATGGGGCCG                                                                             <  SRR4302533.2607094/94‑1 (MQ=60)
                    CGATCACATCATTCCACTCTTGTTCAGGGAACTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAATGGGGCCGAT                                                                           >  SRR4302533.4569507/1‑94 (MQ=60)
                    CGATCACATCATTCCACTCTTGTTCAGGGAACTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAATGGGGCCGAT                                                                           <  SRR4302533.446328/94‑1 (MQ=60)
                      ATCACATCATTCCACTCTTGTTCAGGGAACTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAATGGCGCCGATGT                                                                         >  SRR4302533.773836/1‑94 (MQ=60)
                       TCACATCATTCCACTCTTGTTCAGGGAACTCAGTAAAAGGATGACGGCGCTGGATAGCGGCGTTATTCACTAGCACATCAATGGGGCCGATGTC                                                                        >  SRR4302533.4132064/1‑94 (MQ=60)
                       TCACATCATTCCACTCTTGTTCAGGGAACTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAATGGGGCCGATGTC                                                                        >  SRR4302533.3786634/1‑94 (MQ=60)
                        CACATCATTCCACTCTTGTTCAGGGAACTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAATGGGGCCGATGTCC                                                                       >  SRR4302533.566731/1‑94 (MQ=60)
                          CATCATTCCACTCTTGTTCAGGGAACTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTT                                                                     >  SRR4302533.3726462/1‑94 (MQ=60)
                             CATTCCACTCTTGTTCAGGGAACTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTC                                                                  >  SRR4302533.1181309/1‑94 (MQ=60)
                                 CCACTCTTGTTCAGGGAACTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATA                                                              <  SRR4302533.1029898/94‑1 (MQ=60)
                                  CACTCTTGTTCAGGGAACTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATAT                                                             >  SRR4302533.5240470/1‑94 (MQ=60)
                                  CACTCTTGTTCAGGGAACTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATAT                                                             <  SRR4302533.325427/94‑1 (MQ=60)
                                   ACTCTTGTTCAGGGAACTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATG                                                            >  SRR4302533.887521/1‑94 (MQ=60)
                                     TCTTGTTCAGGGAACTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTT                                                          <  SRR4302533.5639500/94‑1 (MQ=60)
                                     TCTTGTTCAGGGAACTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTT                                                          <  SRR4302533.2714600/94‑1 (MQ=60)
                                      CTTGTTCAGGGAACTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTC                                                         >  SRR4302533.2915083/1‑94 (MQ=60)
                                       TTGTTCAGGGAACTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCA                                                        <  SRR4302533.5519865/94‑1 (MQ=60)
                                       TTGTTCAGGGAACTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCA                                                        <  SRR4302533.4825668/94‑1 (MQ=60)
                                       TTGTTCAGGGAACTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCA                                                        <  SRR4302533.1744228/94‑1 (MQ=60)
                                        TGTTCAGGGAACTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAA                                                       <  SRR4302533.4122146/94‑1 (MQ=60)
                                         GTTCAGGGAACTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAAC                                                      <  SRR4302533.1235305/94‑1 (MQ=60)
                                         GTTCAGGGAACTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAAC                                                      <  SRR4302533.5462603/94‑1 (MQ=60)
                                          TTCAGGGAACTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAACG                                                     >  SRR4302533.399469/1‑94 (MQ=60)
                                          TTCAGGGAACTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAACG                                                     <  SRR4302533.2182356/94‑1 (MQ=60)
                                           TCAGGGAACTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAACGG                                                    <  SRR4302533.2529732/94‑1 (MQ=60)
                                            CAGGGAACTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAACGGC                                                   >  SRR4302533.2370403/1‑94 (MQ=60)
                                             AGGGAACTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAACGGCG                                                  <  SRR4302533.5637924/94‑1 (MQ=60)
                                             AGGGAACTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAACGGCG                                                  <  SRR4302533.5076200/94‑1 (MQ=60)
                                             AGGGAACTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAACGGCG                                                  >  SRR4302533.4296701/1‑94 (MQ=60)
                                             AGGGAACTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAACGGCG                                                  >  SRR4302533.2711234/1‑94 (MQ=60)
                                             AGGGAACTCAGTAAAAGGATGACGGCGCTGGATAACGGCGTTATTCACTAGCACATCAATGGAGCCGATGTCCTTTTCGATATGTTCAACGGCG                                                  >  SRR4302533.4058242/1‑94 (MQ=60)
                                              GGGAACTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAACGGCGG                                                 <  SRR4302533.4848920/94‑1 (MQ=60)
                                              GGGAACTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAACGGCGG                                                 >  SRR4302533.383134/1‑94 (MQ=60)
                                              GGGAACTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAACGGCGG                                                 >  SRR4302533.1485061/1‑94 (MQ=60)
                                                GAACTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAACGGCGGCA                                               >  SRR4302533.588777/1‑94 (MQ=60)
                                                GAACTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAACGGCGGCA                                               >  SRR4302533.4353941/1‑94 (MQ=60)
                                                  ACTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTGACTAGCACATGAATGGGGCGGATGTCCTTTTCGATATGTTCAACGGCGGCATC                                             <  SRR4302533.1417241/94‑1 (MQ=60)
                                                  ACTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAACGGCGGCATC                                             >  SRR4302533.247236/1‑94 (MQ=60)
                                                   CTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAACGGCGGCATCA                                            >  SRR4302533.3130560/1‑94 (MQ=60)
                                                     CAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAACGGCGGCATCAAT                                          >  SRR4302533.5128009/1‑94 (MQ=60)
                                                      AGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAACGGCGGCATCAATT                                         <  SRR4302533.1890775/94‑1 (MQ=60)
                                                      AGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAACGGCGGCATCAATT                                         <  SRR4302533.6343129/94‑1 (MQ=60)
                                                        TAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAACGGCGGCATCAATTTC                                       >  SRR4302533.861678/1‑94 (MQ=60)
                                                        TAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAACGGCGGCATCAATTTC                                       <  SRR4302533.496750/94‑1 (MQ=60)
                                                        TAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAACGGCGGCATCAATTTC                                       >  SRR4302533.5328453/1‑94 (MQ=60)
                                                         AAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAACGGCGGCATCAATTTCA                                      >  SRR4302533.5476686/1‑94 (MQ=60)
                                                         AAAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAACGGCGGCATCAATTTCA                                      >  SRR4302533.1614976/1‑94 (MQ=60)
                                                          AAAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAACGGCGGCATCAATTTCAT                                     <  SRR4302533.720962/94‑1 (MQ=60)
                                                           AAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAACGGCGGCATCAATTTCATG                                    <  SRR4302533.1275635/94‑1 (MQ=60)
                                                           AAGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAACGGCGGCATCAATTTCATG                                    <  SRR4302533.1694502/94‑1 (MQ=60)
                                                            AGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAACGGCGGCATCAATTTCATGT                                   >  SRR4302533.1365842/1‑94 (MQ=60)
                                                            AGGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAACGGCGGCATCAATTTCATGT                                   <  SRR4302533.3058819/94‑1 (MQ=60)
                                                             GGATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAACGGCGGCATCAATTTCATGTT                                  >  SRR4302533.4786857/1‑94 (MQ=60)
                                                              GATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAACGGCGGCATCAATTTCATGTTT                                 <  SRR4302533.3733513/94‑1 (MQ=60)
                                                              GATGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAACGGCGGCATCAATTTCATGTTT                                 <  SRR4302533.5913633/94‑1 (MQ=60)
                                                                TGACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAACGGCGGCATCAATTTCATGTTTAT                               <  SRR4302533.5405438/94‑1 (MQ=60)
                                                                  ACGGCGCTGGATACCGGCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAACGGCGGCATCAATTTCATGTTTATGA                             <  SRR4302533.4035567/94‑1 (MQ=60)
                                                                   CGGCGCTGGATACCGGCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAACGGCGGCATCAATTTCATGTTTATGAG                            <  SRR4302533.512011/94‑1 (MQ=60)
                                                                    GGCGCTGGATACCGGCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAACGGCGGCATCAATTTCATGTTTATGAGT                           >  SRR4302533.4510011/1‑94 (MQ=60)
                                                                    GGCGCTGGATACCGGCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAACGGCGGCATCAATTTCATGTTTATGAGT                           <  SRR4302533.2877284/94‑1 (MQ=60)
                                                                      CGCTGGATACCGGCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAACGGCGGCATCAATTTCATGTTTATGAGTAA                         <  SRR4302533.5874603/94‑1 (MQ=60)
                                                                      CGCTGGATACCGGCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAACGGCGGCATCAATTTCATGTTTATGAGTAA                         >  SRR4302533.2156781/1‑94 (MQ=60)
                                                                        CTGGATACCGGCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAACGGCGGCATCAATTTCATGTTTATGAGTAACA                       >  SRR4302533.2622907/1‑94 (MQ=60)
                                                                         TGGATACCGGCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAACGGCGGCATCAATTTCATGTTTATGAGTAACAT                      >  SRR4302533.1137918/1‑94 (MQ=60)
                                                                         TGGATACCGGCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAACGGCGGCATCAATTTCATGTTTATGAGTAACAT                      <  SRR4302533.4065874/94‑1 (MQ=60)
                                                                           GATACCGGCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAACGGCGGCATCAATTTCATGTTTATGAGTAACATTA                    <  SRR4302533.279143/94‑1 (MQ=60)
                                                                           GATACCGCGGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAACGGCGGCATCAATTTCATGTTTATGAGTAACATTA                    <  SRR4302533.3086464/94‑1 (MQ=60)
                                                                            ATACCGGCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAACGGCGGCATCAATTTCATGTTTATGAGTAACATTAA                   >  SRR4302533.3312562/1‑94 (MQ=60)
                                                                             TACCGGCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAACGGCGGCATCAATTTCATGTTTATGAGTAACATTAAA                  <  SRR4302533.576884/94‑1 (MQ=60)
                                                                              ACCGGCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAACGGCGGCATCAATTTCATGTTTATGAGTAACATTAAAA                 <  SRR4302533.5174771/94‑1 (MQ=60)
                                                                              ACCGGCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAACGGCGGCATCAATTTCATGTTTATGAGTAACATTAAAA                 >  SRR4302533.194719/1‑94 (MQ=60)
                                                                              ACCGGCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAACGGCGGCATCAATTTCATGTTTATGAGTAACATTAAAA                 <  SRR4302533.4697993/94‑1 (MQ=60)
                                                                               CCGGCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAACGGCGGCATCAATTTCATGTTTATGAGTAACATTAAAAG                >  SRR4302533.5687331/1‑94 (MQ=60)
                                                                               CCGGCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAACGGCGGCATCAATTTCATGTTTATGAGTAACATTAAAAG                >  SRR4302533.1229785/1‑94 (MQ=60)
                                                                                CGGCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAACGGCGGCATCAATTTCATGTTTATGAGTAACATTAAAAGG               >  SRR4302533.1863370/1‑94 (MQ=60)
                                                                                 GGCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAACGGCGGCATCAATTTCATGTTTATGAGTAACATTAAAAGGT              <  SRR4302533.3809209/94‑1 (MQ=60)
                                                                                 GGCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAACGGCGGCATCAATTTCATGTTTATGAGTAACATTAAAAGGT              <  SRR4302533.5857686/94‑1 (MQ=60)
                                                                                 GGCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAACGGCGGCATCAATTTCATGTTTATGAGTAACATTAAAAGGT              <  SRR4302533.2630159/94‑1 (MQ=60)
                                                                                  GCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAACGGCGGCATCAATTTCATGTTTATGAGTAACATTAAAAGGTG             <  SRR4302533.1160211/94‑1 (MQ=60)
                                                                                  GCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAACGGCGGCATCAATTTCATGTTTATGAGTAACATTAAAAGGTG             <  SRR4302533.2625190/94‑1 (MQ=60)
                                                                                  GCGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAACGGCGGCATCAATTTCATGTTTATGAGTAACATTAAAAGGTG             >  SRR4302533.2214572/1‑94 (MQ=60)
                                                                                   CGTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAACGGCGGCATCAATTTCATGTTTATGAGTAACATTAAAAGGTGC            <  SRR4302533.1335676/94‑1 (MQ=60)
                                                                                    GTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAACGGCGGCATCAATTTCATGTTTATGAGTAACATTAAAAGGTGCG           >  SRR4302533.3716129/1‑94 (MQ=60)
                                                                                    GTTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAACGGCGGCATCAATTTCATGTTTATGAGTAACATTAAAAGGTGCG           <  SRR4302533.5243759/94‑1 (MQ=60)
                                                                                     TTATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAACGGCGGCATCAATTTCATGTTTATGAGTAACATTAAAAGGTGCGG          >  SRR4302533.1410276/1‑94 (MQ=60)
                                                                                      TATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAACGGCGGCATCAATTTCATGTTTATGAGTAACATTAAAAGGTGCGGC         <  SRR4302533.1618511/94‑1 (MQ=60)
                                                                                       ATTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAACGGCGGCATCAATTTCATGTTTATGAGTAACATTAAAAGGTGCGGCA        >  SRR4302533.1257812/1‑94 (MQ=60)
                                                                                        TTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAACGGCGGCATCAATTTCATGTTTATGAGTAACATTAAAAGGTGCGGCAA       >  SRR4302533.4965527/1‑94 (MQ=60)
                                                                                        TTCACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAACGGCGGCATCAATTTCATGTTTATGAGTAACATTAAAAGGTGCGGCAA       >  SRR4302533.4965526/1‑94 (MQ=60)
                                                                                          CACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAACGGCGGCATCAATTTCATGTTTATGAGTAACATTAAAAGGTGCGGCAACG     >  SRR4302533.4101567/1‑94 (MQ=60)
                                                                                          CACTAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAACGGCGGCATCAATTTCATGTTTATGAGTAACATTAAAAGGTGCGGCAACG     >  SRR4302533.982709/1‑94 (MQ=60)
                                                                                             TAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAACGGCGGCATCAATTTCATGTTTATGAGTAACATTAAAAGGTGCGGCAACGGCC  >  SRR4302533.3605902/1‑94 (MQ=60)
                                                                                             |                                                                                             
CACGGCGGTCTGGTTTACTGCGATCACATCATTCCACTCTTGTTCAGGGAACTCAGTAAAAGGATGACGGCGCTGGATACCGGCGTTATTCACCAGCACATCAATGGGGCCGATGTCCTTTTCGATATGTTCAACGGCGGCATCAATTTCATGTTTATGAGTAACATTAAAAGGTGCGGCAACGGCC  >  NC_000913/4490557‑4490743

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑