Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,502,304 (T)5→6 coding (679/1218 nt) b4279 → putative transport protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,502,2991.T99.6% 845.1 / ‑4.8 226C225F (TGT→TTT) b4279putative transport protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  major base T (118/107);  minor base A (1/0);  total (119/107)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

Only 100 of 248 total aligned reads displayed.

GTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTG‑TTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTC  >  NC_000913/4502207‑4502386
                                                                                             |                                                                                       
gTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTg                                                                                          >  1:825616/1‑93 (MQ=255)
 tGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTG‑t                                                                                        >  1:2724281/1‑93 (MQ=255)
 tGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTG‑t                                                                                        <  1:3728506/93‑1 (MQ=255)
  ggATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTG‑tt                                                                                       >  1:1561172/1‑93 (MQ=255)
   gATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTG‑ttt                                                                                      >  1:6153056/1‑93 (MQ=255)
    aTTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTG‑tttt                                                                                     >  1:772866/1‑93 (MQ=255)
       gAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTG‑ttttttg                                                                                  >  1:5490305/1‑91 (MQ=255)
       gAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTG‑ttttttg                                                                                  >  2:3460968/1‑91 (MQ=255)
       gAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTG‑ttttttg                                                                                  <  1:721606/93‑3 (MQ=255)
           aTAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTa                                                                               <  1:184260/93‑1 (MQ=255)
           aTAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTa                                                                               <  1:3240199/93‑1 (MQ=255)
            tAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTAt                                                                              >  2:1816525/1‑93 (MQ=255)
            tAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTAt                                                                              >  1:1334966/1‑93 (MQ=255)
             aaaTATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTAtt                                                                             <  1:1424693/93‑1 (MQ=255)
             aaaTATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTAtt                                                                             >  2:1368475/1‑93 (MQ=255)
              aaTATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTAttt                                                                            >  2:1960136/1‑93 (MQ=255)
               atatAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTg                                                                           >  1:4357201/1‑93 (MQ=255)
               atatAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTg                                                                           <  1:899009/93‑1 (MQ=255)
                tataAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTgg                                                                          <  2:3702420/93‑1 (MQ=255)
                   aaGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGc                                                                       >  1:1587157/1‑93 (MQ=255)
                   aaGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGc                                                                       >  2:4482616/1‑93 (MQ=255)
                    aGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCa                                                                      <  2:228488/93‑1 (MQ=255)
                     ggATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCaa                                                                     >  2:430122/1‑93 (MQ=255)
                        tAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACt                                                                  >  1:2446495/1‑93 (MQ=255)
                         aaaTCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTg                                                                 >  2:5439960/1‑93 (MQ=255)
                          aaTCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTgg                                                                <  1:4996846/93‑1 (MQ=255)
                           aTCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGc                                                               <  2:3741347/93‑1 (MQ=255)
                           aTCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTACCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGc                                                               <  2:4234353/93‑1 (MQ=255)
                           aTCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTCCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGc                                                               <  2:5554549/93‑1 (MQ=255)
                            tCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGcc                                                              >  1:1474786/1‑93 (MQ=255)
                            tCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGcc                                                              >  2:513444/1‑93 (MQ=255)
                            tCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGcc                                                              <  1:4557731/93‑1 (MQ=255)
                             cAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCg                                                             >  2:3865930/1‑93 (MQ=255)
                             cAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCg                                                             >  1:3835840/1‑93 (MQ=255)
                              aaCATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGa                                                            <  1:5796536/93‑1 (MQ=255)
                              aaCATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGa                                                            >  1:4003760/1‑93 (MQ=255)
                              aaCATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTAGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGa                                                            <  1:3927781/93‑1 (MQ=255)
                               aCATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGAt                                                           <  1:5497601/93‑1 (MQ=255)
                                cATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATa                                                          <  1:3413225/93‑1 (MQ=255)
                                  tttttGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATaaa                                                        >  1:680396/1‑93 (MQ=255)
                                   ttttGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAAc                                                       <  1:1009508/93‑1 (MQ=255)
                                    tttGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACg                                                      <  2:4949443/93‑1 (MQ=255)
                                      tgtctgtcTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGa                                                    <  1:2611157/93‑1 (MQ=255)
                                        tctgtctTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGact                                                  <  1:5684571/93‑1 (MQ=255)
                                          tgtctTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGactac                                                <  1:1021558/93‑1 (MQ=255)
                                          tgtctTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGactac                                                >  1:2584229/1‑93 (MQ=255)
                                            tctTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTActt                                              >  1:4853772/1‑93 (MQ=255)
                                             ctTCAGAAACCCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTActtc                                             <  2:1319093/93‑1 (MQ=255)
                                             ctTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTActtc                                             <  2:785865/93‑1 (MQ=255)
                                               tCAGAAACCCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTActtcct                                           <  2:3172045/93‑1 (MQ=255)
                                               tCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTActtcct                                           >  2:4064664/1‑93 (MQ=255)
                                                cAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTActtcctt                                          >  2:2000253/1‑93 (MQ=255)
                                                 aGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTActtccttc                                         >  2:2655234/1‑93 (MQ=255)
                                                  gAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACttccttcc                                        >  2:2607250/1‑93 (MQ=255)
                                                  gAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACttccttcc                                        <  1:1146938/93‑1 (MQ=255)
                                                     aaCCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTAc                                     >  2:2920258/1‑93 (MQ=255)
                                                     aaCCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTAc                                     >  1:1107690/1‑93 (MQ=255)
                                                      aCCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTAcc                                    >  2:5560811/1‑93 (MQ=255)
                                                       ccACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCt                                   >  1:3510838/1‑93 (MQ=255)
                                                       ccACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCt                                   >  1:3869330/1‑93 (MQ=255)
                                                        cacaTCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTg                                  <  1:4469382/93‑1 (MQ=255)
                                                        cacaTCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTg                                  <  2:4985411/93‑1 (MQ=255)
                                                          caTCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGc                                <  1:1918018/93‑1 (MQ=255)
                                                          caTCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGc                                >  1:2461601/1‑93 (MQ=255)
                                                          caTCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGc                                >  2:6174929/1‑93 (MQ=255)
                                                            tCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAg                              >  2:5973982/1‑93 (MQ=255)
                                                              tttCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGAt                            <  2:3330040/93‑1 (MQ=255)
                                                                tCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATaa                          <  2:3924402/93‑1 (MQ=255)
                                                                tCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATaa                          <  2:2991586/93‑1 (MQ=255)
                                                                  aaTCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATg                        >  2:4800670/1‑93 (MQ=255)
                                                                    tctcTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGa                      >  1:306783/1‑93 (MQ=255)
                                                                    tctcTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGa                      >  1:4047284/1‑93 (MQ=255)
                                                                     ctctATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAg                     <  2:5879110/93‑1 (MQ=255)
                                                                     ctctATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAg                     <  2:1439069/93‑1 (MQ=255)
                                                                      tctATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGt                    >  2:84492/1‑93 (MQ=255)
                                                                      tctATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGt                    <  1:2138723/93‑1 (MQ=255)
                                                                        tATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTa                  <  2:6030054/93‑1 (MQ=255)
                                                                        tATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTa                  >  1:87041/1‑93 (MQ=255)
                                                                        tATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTa                  >  1:1257795/1‑93 (MQ=255)
                                                                         aTGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTaa                 >  2:5485025/1‑93 (MQ=255)
                                                                         aTGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTaa                 >  1:1705064/1‑93 (MQ=255)
                                                                         aTGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTaa                 <  1:1405506/93‑1 (MQ=255)
                                                                          tGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAAt                <  1:2202691/93‑1 (MQ=255)
                                                                           gATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATa               >  1:532628/1‑93 (MQ=255)
                                                                           gATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGCACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATa               <  2:354411/93‑1 (MQ=255)
                                                                            aTCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATAc              >  2:5652177/1‑93 (MQ=255)
                                                                             tCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACa             <  2:2038558/93‑1 (MQ=255)
                                                                               gTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGt           >  2:2330078/1‑93 (MQ=255)
                                                                               gTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGt           <  1:2826616/93‑1 (MQ=255)
                                                                               gTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGt           >  1:6055999/1‑93 (MQ=255)
                                                                                ttttCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTg          <  1:3167100/93‑1 (MQ=255)
                                                                                ttttCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTg          >  2:1226283/1‑93 (MQ=255)
                                                                                ttttCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTg          >  1:5217887/1‑93 (MQ=255)
                                                                                 tttCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTgg         >  1:670842/1‑93 (MQ=255)
                                                                                   tCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTc       >  1:248756/1‑93 (MQ=255)
                                                                                   tCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTc       >  2:5909329/1‑93 (MQ=255)
                                                                                     cTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTGCTACCTGGCAGATAATGGAGTTAATACAGTGGTCAt     <  2:5774056/93‑1 (MQ=255)
                                                                                     cTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCAt     >  2:5298410/1‑93 (MQ=255)
                                                                                     cTCGTCTGATTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCAt     >  2:6152191/1‑93 (MQ=255)
                                                                                      tCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCAtt    >  1:2051735/1‑93 (MQ=255)
                                                                                             |                                                                                       
GTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTG‑TTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTC  >  NC_000913/4502207‑4502386

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTG‑TTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGA  >  NC_000913/4502211‑4502397
                                                                                              |                                                                                             
ATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTG‑TTTTT                                                                                               >  SRR4302533.4485869/1‑94 (MQ=60)
 TTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTG‑TTTTTT                                                                                              <  SRR4302533.2612386/94‑1 (MQ=60)
 TTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTG‑TTTTTT                                                                                              <  SRR4302533.3095499/94‑1 (MQ=60)
  TGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTG‑TTTTTtg                                                                                             >  SRR4302533.5523402/1‑92 (MQ=60)
  TGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTG‑TTTTTtg                                                                                             <  SRR4302533.1437226/94‑3 (MQ=60)
   GAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTG‑TTTTTtgt                                                                                            <  SRR4302533.725865/94‑4 (MQ=60)
    AAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTC                                                                                            >  SRR4302533.643253/1‑94 (MQ=60)
      GATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTA                                                                                          <  SRR4302533.731303/94‑1 (MQ=60)
      AATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTA                                                                                          <  SRR4302533.5200597/94‑1 (MQ=60)
       ATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTAT                                                                                         <  SRR4302533.3260165/94‑1 (MQ=60)
       ATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTAT                                                                                         >  SRR4302533.1342976/1‑94 (MQ=60)
       ATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTAT                                                                                         <  SRR4302533.1940975/94‑1 (MQ=60)
       ATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTAT                                                                                         <  SRR4302533.185406/94‑1 (MQ=60)
         AAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTT                                                                                       >  SRR4302533.837804/1‑94 (MQ=60)
         AAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTT                                                                                       <  SRR4302533.1433311/94‑1 (MQ=60)
          AATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTG                                                                                      >  SRR4302533.4383501/1‑94 (MQ=60)
          AATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTG                                                                                      <  SRR4302533.87432/94‑1 (MQ=60)
          AATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTG                                                                                      <  SRR4302533.1079180/94‑1 (MQ=60)
           ATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGG                                                                                     >  SRR4302533.708422/1‑94 (MQ=60)
           ATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGG                                                                                     <  SRR4302533.904284/94‑1 (MQ=60)
            TATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGT                                                                                    <  SRR4302533.244025/94‑1 (MQ=60)
            TATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGT                                                                                    >  SRR4302533.688145/1‑94 (MQ=60)
              TAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGC                                                                                  >  SRR4302533.1596734/1‑94 (MQ=60)
               AAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCA                                                                                 >  SRR4302533.2622038/1‑94 (MQ=60)
                AGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAA                                                                                >  SRR4302533.3866670/1‑94 (MQ=60)
                 GGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAA                                                                               >  SRR4302533.3936452/1‑94 (MQ=60)
                 GGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAA                                                                               >  SRR4302533.3105701/1‑94 (MQ=60)
                  GATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAAC                                                                              <  SRR4302533.5304567/94‑1 (MQ=60)
                  GATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAAC                                                                              <  SRR4302533.112626/94‑1 (MQ=60)
                   ATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACT                                                                             >  SRR4302533.2461594/1‑94 (MQ=60)
                   ATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACT                                                                             <  SRR4302533.1415617/94‑1 (MQ=60)
                    TAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTG                                                                            <  SRR4302533.1752236/94‑1 (MQ=60)
                    TAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTG                                                                            >  SRR4302533.212139/1‑94 (MQ=60)
                     AAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGG                                                                           >  SRR4302533.735355/1‑94 (MQ=60)
                     AAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGG                                                                           >  SRR4302533.4038337/1‑94 (MQ=60)
                     AAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGG                                                                           <  SRR4302533.1427948/94‑1 (MQ=60)
                     AAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGG                                                                           <  SRR4302533.879403/94‑1 (MQ=60)
                      AATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGC                                                                          <  SRR4302533.5027141/94‑1 (MQ=60)
                      AATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGC                                                                          >  SRR4302533.5029926/1‑94 (MQ=60)
                      AATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGC                                                                          <  SRR4302533.2497311/94‑1 (MQ=60)
                       ATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCC                                                                         >  SRR4302533.1483701/1‑94 (MQ=60)
                        TCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCG                                                                        <  SRR4302533.4585370/94‑1 (MQ=60)
                        TCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCG                                                                        >  SRR4302533.3859278/1‑94 (MQ=60)
                         CAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGA                                                                       >  SRR4302533.4028137/1‑94 (MQ=60)
                          AACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGAT                                                                      >  SRR4302533.102264/1‑94 (MQ=60)
                          AACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGAT                                                                      >  SRR4302533.2703069/1‑94 (MQ=60)
                          AACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGAT                                                                      <  SRR4302533.5831557/94‑1 (MQ=60)
                          AACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGAT                                                                      <  SRR4302533.7962/94‑1 (MQ=60)
                          AACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTAGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGAT                                                                      <  SRR4302533.3951761/94‑1 (MQ=60)
                           ACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATA                                                                     <  SRR4302533.5530759/94‑1 (MQ=60)
                            CATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAA                                                                    <  SRR4302533.3434309/94‑1 (MQ=60)
                            CATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAA                                                                    >  SRR4302533.2467809/1‑94 (MQ=60)
                             ATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAA                                                                   >  SRR4302533.954130/1‑94 (MQ=60)
                             ATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAA                                                                   >  SRR4302533.684422/1‑94 (MQ=60)
                             ATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAA                                                                   <  SRR4302533.2511533/94‑1 (MQ=60)
                               TTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACG                                                                 <  SRR4302533.1015413/94‑1 (MQ=60)
                               TTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACG                                                                 <  SRR4302533.1304771/94‑1 (MQ=60)
                               TTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACG                                                                 >  SRR4302533.5525997/1‑94 (MQ=60)
                               TTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACG                                                                 <  SRR4302533.1591321/94‑1 (MQ=60)
                               TTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACG                                                                 <  SRR4302533.18562/94‑1 (MQ=60)
                                TTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGG                                                                <  SRR4302533.1520056/94‑1 (MQ=60)
                                  TGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGAC                                                              <  SRR4302533.2627183/94‑1 (MQ=60)
                                   GTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACT                                                             >  SRR4302533.4389915/1‑94 (MQ=60)
                                    TCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTA                                                            <  SRR4302533.5718907/94‑1 (MQ=60)
                                     CTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTAC                                                           <  SRR4302533.4962731/94‑1 (MQ=60)
                                     CTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTAC                                                           >  SRR4302533.2600119/1‑94 (MQ=60)
                                      TGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACT                                                          <  SRR4302533.1027560/94‑1 (MQ=60)
                                       GTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTT                                                         >  SRR4302533.4883188/1‑94 (MQ=60)
                                        TCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTC                                                        <  SRR4302533.1685018/94‑1 (MQ=60)
                                          TTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCT                                                      >  SRR4302533.226603/1‑94 (MQ=60)
                                             AGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCC                                                   <  SRR4302533.3059421/94‑1 (MQ=60)
                                              GAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCT                                                  <  SRR4302533.1153784/94‑1 (MQ=60)
                                              GAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCT                                                  <  SRR4302533.384880/94‑1 (MQ=60)
                                                AAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTTC                                                <  SRR4302533.613782/94‑1 (MQ=60)
                                                AAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTAC                                                >  SRR4302533.1114258/1‑94 (MQ=60)
                                                AAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTAC                                                >  SRR4302533.1221746/1‑94 (MQ=60)
                                                  ACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCT                                              >  SRR4302533.3532480/1‑94 (MQ=60)
                                                  ACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCT                                              >  SRR4302533.4043694/1‑94 (MQ=60)
                                                  ACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCT                                              >  SRR4302533.3892952/1‑94 (MQ=60)
                                                   CCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTG                                             >  SRR4302533.1129414/1‑94 (MQ=60)
                                                    CACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGG                                            <  SRR4302533.4496414/94‑1 (MQ=60)
                                                     ACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGC                                           <  SRR4302533.5558645/94‑1 (MQ=60)
                                                     ACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGC                                           >  SRR4302533.2476787/1‑94 (MQ=60)
                                                     ACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGC                                           >  SRR4302533.5064232/1‑94 (MQ=60)
                                                     ACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGC                                           >  SRR4302533.4727205/1‑94 (MQ=60)
                                                     ACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGC                                           >  SRR4302533.2786490/1‑94 (MQ=60)
                                                     ACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGC                                           >  SRR4302533.2786489/1‑94 (MQ=60)
                                                     ACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGC                                           >  SRR4302533.2187208/1‑94 (MQ=60)
                                                      CATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCA                                          <  SRR4302533.1929727/94‑1 (MQ=60)
                                                       ATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAG                                         <  SRR4302533.3986254/94‑1 (MQ=60)
                                                       ATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAG                                         >  SRR4302533.6297406/1‑94 (MQ=60)
                                                       ATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAG                                         >  SRR4302533.1703914/1‑94 (MQ=60)
                                                           TTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAA                                     <  SRR4302533.1275408/94‑1 (MQ=60)
                                                               ATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGA                                 >  SRR4302533.5827916/1‑94 (MQ=60)
                                                               ATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGA                                 >  SRR4302533.4071928/1‑94 (MQ=60)
                                                               ATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGA                                 >  SRR4302533.4231653/1‑94 (MQ=60)
                                                               ATCTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGA                                 >  SRR4302533.308659/1‑94 (MQ=60)
                                                                 CTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCGAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGT                               >  SRR4302533.3404425/1‑94 (MQ=60)
                                                                 CTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGT                               >  SRR4302533.4758225/1‑94 (MQ=60)
                                                                 CTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGT                               >  SRR4302533.3816425/1‑94 (MQ=60)
                                                                 CTCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGT                               >  SRR4302533.1904337/1‑94 (MQ=60)
                                                                  TCTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTT                              <  SRR4302533.2151908/94‑1 (MQ=60)
                                                                   CTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTA                             >  SRR4302533.1265316/1‑94 (MQ=60)
                                                                   CTATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTA                             >  SRR4302533.87600/1‑94 (MQ=60)
                                                                    TATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAA                            <  SRR4302533.5656548/94‑1 (MQ=60)
                                                                    TATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAA                            >  SRR4302533.1715390/1‑94 (MQ=60)
                                                                     ATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAAT                           <  SRR4302533.3896114/94‑1 (MQ=60)
                                                                     ATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAAT                           >  SRR4302533.2139518/1‑94 (MQ=60)
                                                                     ATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAAT                           <  SRR4302533.1414009/94‑1 (MQ=60)
                                                                     ATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAAT                           >  SRR4302533.5399236/1‑94 (MQ=60)
                                                                     ATGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGACAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAAT                           <  SRR4302533.5844915/94‑1 (MQ=60)
                                                                      TGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATA                          <  SRR4302533.2216268/94‑1 (MQ=60)
                                                                      TGATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATA                          >  SRR4302533.535796/1‑94 (MQ=60)
                                                                        ATCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACA                        <  SRR4302533.4545530/94‑1 (MQ=60)
                                                                         TCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAG                       <  SRR4302533.1403510/94‑1 (MQ=60)
                                                                         TCGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAG                       <  SRR4302533.2488360/94‑1 (MQ=60)
                                                                          CGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGT                      >  SRR4302533.5452524/1‑94 (MQ=60)
                                                                          CGTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGT                      >  SRR4302533.6092672/1‑94 (MQ=60)
                                                                           GTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTG                     <  SRR4302533.2844008/94‑1 (MQ=60)
                                                                           GTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTG                     >  SRR4302533.3526966/1‑94 (MQ=60)
                                                                           GTTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTG                     >  SRR4302533.5249297/1‑94 (MQ=60)
                                                                            TTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGG                    >  SRR4302533.674804/1‑94 (MQ=60)
                                                                            TTTTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGG                    <  SRR4302533.3186587/94‑1 (MQ=60)
                                                                              TTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAGCGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTC                  <  SRR4302533.5910255/94‑1 (MQ=60)
                                                                              TTCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTC                  >  SRR4302533.250291/1‑94 (MQ=60)
                                                                               TCCTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCA                 >  SRR4302533.3463376/1‑94 (MQ=60)
                                                                                 CTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATT               >  SRR4302533.2064396/1‑94 (MQ=60)
                                                                                 CTCGTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATT               >  SRR4302533.1537329/1‑94 (MQ=60)
                                                                                    GTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCA            >  SRR4302533.4052300/1‑94 (MQ=60)
                                                                                    GTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCA            >  SRR4302533.2535650/1‑94 (MQ=60)
                                                                                    GTCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCA            <  SRR4302533.675715/94‑1 (MQ=60)
                                                                                     TCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAA           <  SRR4302533.328221/94‑1 (MQ=60)
                                                                                     TCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAA           >  SRR4302533.6239738/1‑94 (MQ=60)
                                                                                     TCTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTNCCTACCTGGCAGATNATGNNNTNAATACAGTGGNNNNNTNAA           >  SRR4302533.5489463/1‑94 (MQ=60)
                                                                                      CTGTTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGTACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAAC          <  SRR4302533.140274/94‑1 (MQ=60)
                                                                                        T‑TTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCT       >  SRR4302533.1967129/1‑94 (MQ=60)
                                                                                            TTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGAT    <  SRR4302533.5356253/94‑1 (MQ=60)
                                                                                            TTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGAT    <  SRR4302533.1868680/94‑1 (MQ=60)
                                                                                             TTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATG   <  SRR4302533.1264200/94‑1 (MQ=60)
                                                                                             TTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATG   >  SRR4302533.3967951/1‑94 (MQ=60)
                                                                                             TTGTCTATTAGGTGCAAGCTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATG   <  SRR4302533.5937384/94‑1 (MQ=60)
                                                                                              TGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGA  <  SRR4302533.2282784/94‑1 (MQ=60)
                                                                                              |                                                                                             
ATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTG‑TTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGA  >  NC_000913/4502211‑4502397

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑