Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,554,777	A→G	A72A (GCA→GCG)	b4326 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,554,777	0	A	G	100.0%	950.1 / NA	293	A72A (GCA→GCG)	b4326	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (140/153); total (140/153)

Only 100 of 297 total aligned reads displayed.

ATTACTTGTTTCAGGCATGCCCGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCACTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACACTCTGGCGATCAGCCTTGAGATAAT  >  NC_000913/4554687‑4554866

aTTACTTGTTTCAGGCATGCCCGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCt                                                                                         >  2:3823667/1‑93 (MQ=255)

aTTACTTGTTTCAGGCATGCCCGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCt                                                                                         >  2:3275823/1‑93 (MQ=255)

aTTACTTGTTTCAGGCATGCCCGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCt                                                                                         >  1:3070271/1‑93 (MQ=255)

 ttACTTGTTTCAGGCATGCCCGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTg                                                                                        <  1:722674/93‑1 (MQ=255)

  tACTTGTTTCAGGCATGCCCGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGCTCTGCGCTGt                                                                                       >  1:4306189/1‑93 (MQ=255)

    cTTGTTTCAGGCATGCCCGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTa                                                                                     <  2:6212758/93‑1 (MQ=255)

     ttGTTTCAGGCATGCCCGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTAt                                                                                    >  1:4847768/1‑93 (MQ=255)

      tGTTTCAGGCATGCCCGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTAtt                                                                                   <  1:4376850/93‑1 (MQ=255)

      tGTTTCAGGCATGCCCGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTAtt                                                                                   <  1:840105/93‑1 (MQ=255)

       gTTTCAGGCATGCCCGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTAttt                                                                                  >  1:3734881/1‑93 (MQ=255)

       gTTTCAGGCATGCCCGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTAttt                                                                                  <  2:4296756/93‑1 (MQ=255)

        tttCAGGCATGCCCGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTc                                                                                 <  1:50468/93‑1 (MQ=255)

        tttCAGGCATGCCCGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTc                                                                                 <  1:5574739/93‑1 (MQ=255)

           cAGGCATGCCCGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATg                                                                              <  2:4144360/93‑1 (MQ=255)

           cAGGCATGCCCGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATg                                                                              <  1:2588776/93‑1 (MQ=255)

                aTGCCCGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCa                                                                         >  2:4458152/1‑93 (MQ=255)

                aTGCCCGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCa                                                                         >  2:5632557/1‑93 (MQ=255)

                 tGCCCGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCAc                                                                        <  2:4990573/93‑1 (MQ=255)

                  gCCCGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACg                                                                       <  2:4726705/93‑1 (MQ=255)

                   cccGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACgg                                                                      >  1:2189543/1‑93 (MQ=255)

                    ccGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGa                                                                     <  1:6016054/93‑1 (MQ=255)

                     cGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGAt                                                                    >  2:4919382/1‑93 (MQ=255)

                     cGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGAt                                                                    <  1:5352018/93‑1 (MQ=255)

                     cGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGAt                                                                    >  1:4110151/1‑93 (MQ=255)

                      gAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGAtt                                                                   >  2:5712339/1‑93 (MQ=255)

                       aGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTg                                                                  <  1:4595128/93‑1 (MQ=255)

                        gTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTgc                                                                 >  2:2087441/1‑93 (MQ=255)

                         tGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTgcg                                                                <  1:3095732/93‑1 (MQ=255)

                           gCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTgcggc                                                              >  2:5568770/1‑93 (MQ=255)

                           gCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTgcggc                                                              >  1:5167977/1‑93 (MQ=255)

                           gCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTgcggc                                                              >  2:1054957/1‑93 (MQ=255)

                             aaGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGt                                                            <  2:3005695/93‑1 (MQ=255)

                             aaGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGt                                                            <  2:4699149/93‑1 (MQ=255)

                             aaGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGt                                                            >  1:5802407/1‑93 (MQ=255)

                              aGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGtt                                                           <  2:3114022/93‑1 (MQ=255)

                                tGCATAACCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGc                                                         >  2:1263249/1‑93 (MQ=255)

                                 gCATAACCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGcg                                                        >  2:1595341/1‑92 (MQ=255)

                                 gCATAACCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCa                                                        >  1:3181173/1‑93 (MQ=255)

                                  cATAACCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAg                                                       <  1:1070128/93‑1 (MQ=255)

                                   aTAACCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAgg                                                      >  1:5949980/1‑93 (MQ=255)

                                   aTAACCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAgg                                                      <  2:1472629/93‑1 (MQ=255)

                                    tAACCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGt                                                     <  2:5412541/93‑1 (MQ=255)

                                     aaCCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGCTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGtt                                                    >  1:3349251/1‑93 (MQ=255)

                                       cccGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTaa                                                  >  1:3494580/1‑93 (MQ=255)

                                        ccGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAAt                                                 >  1:2089288/1‑93 (MQ=255)

                                        ccGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAAt                                                 <  2:1315264/93‑1 (MQ=255)

                                          gAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAAttt                                               <  2:4768291/93‑1 (MQ=255)

                                            gCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGa                                             >  2:3572058/1‑93 (MQ=255)

                                             cGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGaa                                            <  1:2072963/93‑1 (MQ=255)

                                               aCAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAaag                                          >  2:3660342/1‑93 (MQ=255)

                                               aCAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAaag                                          >  2:4958883/1‑93 (MQ=255)

                                                cAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAaaga                                         <  2:2437538/93‑1 (MQ=255)

                                                cAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAaaga                                         >  1:2037502/1‑93 (MQ=255)

                                                cAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAaaga                                         <  1:5003605/93‑1 (MQ=255)

                                                cAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAaaga                                         >  1:1150895/1‑93 (MQ=255)

                                                 aaaCATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAaagaa                                        <  1:5410697/93‑1 (MQ=255)

                                                  aaCATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAaagaag                                       <  2:5912123/93‑1 (MQ=255)

                                                  aaCATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAaagaag                                       <  2:629625/93‑1 (MQ=255)

                                                  aaCATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAaagaag                                       >  2:2826051/1‑93 (MQ=255)

                                                  aaCATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAaagaag                                       >  2:1880824/1‑93 (MQ=255)

                                                    cATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCg                                     >  1:784242/1‑93 (MQ=255)

                                                     aTCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGt                                    >  1:4469252/1‑93 (MQ=255)

                                                      tctcCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTa                                   >  1:1566717/1‑93 (MQ=255)

                                                      tctcCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTa                                   <  1:3896965/93‑1 (MQ=255)

                                                      tctcCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTa                                   <  1:2720312/93‑1 (MQ=255)

                                                      tctcCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTa                                   <  1:6124323/93‑1 (MQ=255)

                                                      tctcCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTa                                   <  1:1657221/93‑1 (MQ=255)

                                                       ctcCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTAc                                  <  2:2236953/93‑1 (MQ=255)

                                                         ccAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTg                                <  1:4298075/93‑1 (MQ=255)

                                                            gagCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCca                             <  2:2764657/93‑1 (MQ=255)

                                                               cTGGTATTGCCGTTAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTTCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTACCacac                          >  2:804498/1‑93 (MQ=255)

                                                               cTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCacac                          >  2:5186343/1‑93 (MQ=255)

                                                                tGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACAct                         <  1:1129801/93‑1 (MQ=255)

                                                                  gTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACActct                       <  2:2510141/93‑1 (MQ=255)

                                                                   tATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACACTCTg                      <  2:4206671/93‑1 (MQ=255)

                                                                   tATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACACTCTg                      <  1:18921/93‑1 (MQ=255)

                                                                   tATTCCCGTGAGCTGCGTTCTGCGCTGTTATTGCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACACTCTg                      <  2:6001671/93‑1 (MQ=255)

                                                                    aTTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACACTCTgg                     <  2:5515303/93‑1 (MQ=255)

                                                                     ttGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACACTCTGGc                    <  1:1306253/93‑1 (MQ=255)

                                                                       gCCGTCAGGTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGCACTGCCACACTCTGGCgg                  >  2:5827138/1‑92 (MQ=255)

                                                                       gCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACACTCTGGCGa                  <  2:6093075/93‑1 (MQ=255)

                                                                        ccGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACACTCTGGCGAt                 >  2:2946508/1‑93 (MQ=255)

                                                                        ccGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACACTCTGGCGAt                 <  2:2011668/93‑1 (MQ=255)

                                                                          gTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACACTCTGGCGATCa               >  2:5316471/1‑93 (MQ=255)

                                                                          gTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACACTCTGGCGATCa               >  1:4012025/1‑93 (MQ=255)

                                                                          gTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGGTGCAGGTTAATTTGAAAGAAGCGTACTGCCACACTCTGGCGATCa               >  2:2397328/1‑93 (MQ=255)

                                                                           tCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACACTCTGGCGATCAg              <  2:320076/93‑1 (MQ=255)

                                                                           tCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACACTCTGGCGATCAg              >  1:5881675/1‑93 (MQ=255)

                                                                             aGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACACTCTGGCGATCAGcc            >  1:4740920/1‑93 (MQ=255)

                                                                              gCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACACTCTGGCGATCAGCCt           <  1:4213868/93‑1 (MQ=255)

                                                                              gCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACACTCTGGCGATCAGCCt           <  1:2842532/93‑1 (MQ=255)

                                                                              gCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACACTCTGGCGATCAGCCt           <  1:1205819/93‑1 (MQ=255)

                                                                               cTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACACTCTGGCGATCAGCCtt          <  2:6194889/93‑1 (MQ=255)

                                                                               cTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACACTCTGGCGATCAGCCtt          >  2:88268/1‑93 (MQ=255)

                                                                                    ttCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACACTCTGGCGATCAGCCTTGAGAt     >  1:3397599/1‑93 (MQ=255)

                                                                                    ttCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACACTCTGGCGATCAGCCTTGAGAt     <  1:5737635/93‑1 (MQ=255)

                                                                                    ttCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACACTCTGGCGATCAGCCTTGAGAt     >  2:959377/1‑93 (MQ=255)

                                                                                     tCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACACTCTGGCGATCAGCCTTGAGATa    <  1:6243750/93‑1 (MQ=255)

                                                                                      cTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACACTCTGGCGATCAGCCTTGAGATaa   <  2:1741828/93‑1 (MQ=255)

                                                                                       tGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACACTCTGGCGATCAGCCTTGAGATAAt  >  2:5460647/1‑93 (MQ=255)

ATTACTTGTTTCAGGCATGCCCGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCACTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACACTCTGGCGATCAGCCTTGAGATAAT  >  NC_000913/4554687‑4554866

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GTCATTACTTGTTTCAGGCATGCCCGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCACTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACACTCTGGCGATCAGCCTTGAGATAATGCTG  >  NC_000913/4554684‑4554870

GTCATTACTTGTTTCAGGCATGCCCGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCG                                                                                               <  SRR4302533.2036431/94‑1 (MQ=60)

GTCATTACTTGTTTCAGGCATGCCCGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCG                                                                                               <  SRR4302533.1690301/94‑1 (MQ=60)

 TCATTACTTGTTTCAGGCATGCCCGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGC                                                                                              >  SRR4302533.4241088/1‑94 (MQ=60)

  CATTACTTGTTTCAGGCATGCCCGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCT                                                                                             >  SRR4302533.3089121/1‑94 (MQ=60)

    TTACTTGTTTCAGGCATGCCCGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGT                                                                                           <  SRR4302533.726948/94‑1 (MQ=60)

    TTACTTGTTTCAGGCATGCCCGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGCTCTGCGCTGT                                                                                           >  SRR4302533.4332098/1‑94 (MQ=60)

     TACTTGTTTCAGGCATGCCCGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTT                                                                                          >  SRR4302533.4271828/1‑94 (MQ=60)

       CTTGTTTCAGGCATGCCCGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTAT                                                                                        >  SRR4302533.4877146/1‑94 (MQ=60)

       CTTGTTTCAGGCATGCCCGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTAT                                                                                        <  SRR4302533.4355859/94‑1 (MQ=60)

       CTTGTTTCAGGCATGCCCGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTAT                                                                                        >  SRR4302533.4734341/1‑94 (MQ=60)

       CTTGTTTCAGGCATGCCCGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTAT                                                                                        <  SRR4302533.6040695/94‑1 (MQ=60)

       CTTGTTTCAGGCATGCCCGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTAT                                                                                        <  SRR4302533.4087291/94‑1 (MQ=60)

       CTTGTTTCAGGCATGCCCGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTAT                                                                                        <  SRR4302533.5478509/94‑1 (MQ=60)

         TGTTTCAGGCATGCCCGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTT                                                                                      <  SRR4302533.845019/94‑1 (MQ=60)

         TGTTTCAGGCATGCCCGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTT                                                                                      <  SRR4302533.4403272/94‑1 (MQ=60)

         TGTTTCAGGCATGCCCGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTT                                                                                      >  SRR4302533.3757794/1‑94 (MQ=60)

          GTTTCAGGCATGCCCGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTC                                                                                     <  SRR4302533.5878761/94‑1 (MQ=60)

          GTTTCAGGCATGCCCGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTC                                                                                     <  SRR4302533.1642236/94‑1 (MQ=60)

          GTTTCAGGCATGCCCGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTC                                                                                     <  SRR4302533.257820/94‑1 (MQ=60)

          GTTTCAGGCATGCCCGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTC                                                                                     <  SRR4302533.2743062/94‑1 (MQ=60)

           TTTCAGGCATGCCCGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCA                                                                                    <  SRR4302533.50813/94‑1 (MQ=60)

           TTTCAGGCATGCCCGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCA                                                                                    <  SRR4302533.5608427/94‑1 (MQ=60)

            TTCAGGCATGCCCGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCAT                                                                                   <  SRR4302533.2705625/94‑1 (MQ=60)

            TTCAGGCATGCCCGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCAT                                                                                   <  SRR4302533.4932357/94‑1 (MQ=60)

              CAGGCATGCCCGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGA                                                                                 <  SRR4302533.3814460/94‑1 (MQ=60)

              CAGGCATGCCCGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGA                                                                                 <  SRR4302533.2604688/94‑1 (MQ=60)

               AGGCATGCCCGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAG                                                                                >  SRR4302533.4404143/1‑94 (MQ=60)

               AGGCATGCCCGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAC                                                                                >  SRR4302533.5018357/1‑94 (MQ=60)

                GGCATGCCCGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGC                                                                               <  SRR4302533.3666663/94‑1 (MQ=60)

                     GCCCGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGG                                                                          <  SRR4302533.2201533/94‑1 (MQ=60)

                     GCCCGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGG                                                                          >  SRR4302533.2203031/1‑94 (MQ=60)

                       CCGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGAT                                                                        <  SRR4302533.5984029/94‑1 (MQ=60)

                       CCGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGAT                                                                        >  SRR4302533.2083620/1‑94 (MQ=60)

                       CCGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGAT                                                                        >  SRR4302533.1969895/1‑94 (MQ=60)

                       CCGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGAT                                                                        <  SRR4302533.6052509/94‑1 (MQ=60)

                       CCGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGAT                                                                        >  SRR4302533.4135135/1‑94 (MQ=60)

                       CCGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGAT                                                                        >  SRR4302533.4135133/1‑94 (MQ=60)

                        CGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATT                                                                       <  SRR4302533.5384131/94‑1 (MQ=60)

                        CGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATT                                                                       <  SRR4302533.878409/94‑1 (MQ=60)

                        CGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATT                                                                       <  SRR4302533.4388093/94‑1 (MQ=60)

                         GAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTG                                                                      >  SRR4302533.1639291/1‑94 (MQ=60)

                          AGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGC                                                                     <  SRR4302533.4622964/94‑1 (MQ=60)

                          AGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGC                                                                     >  SRR4302533.2059590/1‑94 (MQ=60)

                          AGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGC                                                                     <  SRR4302533.8462/94‑1 (MQ=60)

                          AGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGC                                                                     <  SRR4302533.1161603/94‑1 (MQ=60)

                           GTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCG                                                                    <  SRR4302533.307612/94‑1 (MQ=60)

                            TGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGG                                                                   <  SRR4302533.3114762/94‑1 (MQ=60)

                             GGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGC                                                                  >  SRR4302533.5199173/1‑94 (MQ=60)

                               CAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGT                                                                >  SRR4302533.5837456/1‑94 (MQ=60)

                                AAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTT                                                               >  SRR4302533.4525902/1‑94 (MQ=60)

                                AAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTT                                                               <  SRR4302533.3127778/94‑1 (MQ=60)

                                AAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTT                                                               >  SRR4302533.1144693/1‑94 (MQ=60)

                                  GTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGC                                                             <  SRR4302533.745252/94‑1 (MQ=60)

                                  GTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGC                                                             <  SRR4302533.3114150/94‑1 (MQ=60)

                                   TGCATAACCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCA                                                            >  SRR4302533.3200744/1‑94 (MQ=60)

                                    GCATAACCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAG                                                           <  SRR4302533.4686163/94‑1 (MQ=60)

                                     CATAACCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGG                                                          <  SRR4302533.1076433/94‑1 (MQ=60)

                                     CATAACCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGG                                                          >  SRR4302533.5985965/1‑94 (MQ=60)

                                      ATAACCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGT                                                         >  SRR4302533.783813/1‑94 (MQ=60)

                                       TAACCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTT                                                        >  SRR4302533.2619032/1‑94 (MQ=60)

                                       TAACCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTT                                                        <  SRR4302533.1163610/94‑1 (MQ=60)

                                       TAACCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGCTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTT                                                        >  SRR4302533.3369944/1‑94 (MQ=60)

                                        AACCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTA                                                       >  SRR4302533.5171395/1‑94 (MQ=60)

                                         ACCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAA                                                      >  SRR4302533.3516134/1‑94 (MQ=60)

                                          CCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAAT                                                     >  SRR4302533.2102151/1‑94 (MQ=60)

                                          CCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGATAAT                                                     >  SRR4302533.1139388/1‑94 (MQ=60)

                                          CCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTCGCAGGTTAAT                                                     >  SRR4302533.4873562/1‑94 (MQ=60)

                                           CCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATT                                                    >  SRR4302533.1740189/1‑94 (MQ=60)

                                           CCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATT                                                    >  SRR4302533.2006408/1‑94 (MQ=60)

                                            CGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTT                                                   >  SRR4302533.5346585/1‑94 (MQ=60)

                                               GCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAA                                                <  SRR4302533.5822044/94‑1 (MQ=60)

                                                CGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAA                                               <  SRR4302533.2085731/94‑1 (MQ=60)

                                                  ACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGA                                             >  SRR4302533.1157790/1‑94 (MQ=60)

                                                  ACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGA                                             >  SRR4302533.2050066/1‑94 (MQ=60)

                                                   CAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAA                                            <  SRR4302533.3224613/94‑1 (MQ=60)

                                                   CAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAA                                            >  SRR4302533.3780069/1‑94 (MQ=60)

                                                   CAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAA                                            >  SRR4302533.1242710/1‑94 (MQ=60)

                                                   CAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAA                                            <  SRR4302533.5033930/94‑1 (MQ=60)

                                                   CAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAA                                            >  SRR4302533.6232/1‑94 (MQ=60)

                                                    AAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAG                                           <  SRR4302533.5443228/94‑1 (MQ=60)

                                                     AACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGC                                          <  SRR4302533.6120795/94‑1 (MQ=60)

                                                     AACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGC                                          <  SRR4302533.3143201/94‑1 (MQ=60)

                                                     AACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGC                                          <  SRR4302533.3934115/94‑1 (MQ=60)

                                                     AACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCAAGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGC                                          >  SRR4302533.6090309/1‑94 (MQ=60)

                                                      ACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCG                                         <  SRR4302533.2500106/94‑1 (MQ=60)

                                                      ACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCG                                         >  SRR4302533.2100508/1‑94 (MQ=60)

                                                      ACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCG                                         >  SRR4302533.788855/1‑94 (MQ=60)

                                                       CATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGT                                        <  SRR4302533.641789/94‑1 (MQ=60)

                                                       CATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGT                                        >  SRR4302533.4837218/1‑94 (MQ=60)

                                                       CATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGT                                        >  SRR4302533.4496283/1‑94 (MQ=60)

                                                        ATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTA                                       >  SRR4302533.1576150/1‑94 (MQ=60)

                                                         TCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTAC                                      <  SRR4302533.6161239/94‑1 (MQ=60)

                                                         TCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTAC                                      <  SRR4302533.2737008/94‑1 (MQ=60)

                                                         TCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTAC                                      <  SRR4302533.1667249/94‑1 (MQ=60)

                                                         TCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTAC                                      <  SRR4302533.3944338/94‑1 (MQ=60)

                                                         TCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTAC                                      <  SRR4302533.3920744/94‑1 (MQ=60)

                                                          CTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACT                                     >  SRR4302533.808538/1‑94 (MQ=60)

                                                          CTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACT                                     <  SRR4302533.5882778/94‑1 (MQ=60)

                                                            CCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGC                                   <  SRR4302533.4323923/94‑1 (MQ=60)

                                                             CAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCC                                  <  SRR4302533.6245892/94‑1 (MQ=60)

                                                                  CTGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAACCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACACT                             >  SRR4302533.5055019/1‑94 (MQ=60)

                                                                   TGGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACACTC                            <  SRR4302533.1136515/94‑1 (MQ=60)

                                                                    GGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACACTCT                           >  SRR4302533.3102814/1‑94 (MQ=60)

                                                                    GGTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTACGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACACTCT                           <  SRR4302533.3410930/94‑1 (MQ=60)

                                                                     GTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACACTCTG                          <  SRR4302533.2129719/94‑1 (MQ=60)

                                                                     GTATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACACTCTG                          <  SRR4302533.1843087/94‑1 (MQ=60)

                                                                     GTATTCCCCTCAGCTGCTTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACACTCTG                          <  SRR4302533.5161877/94‑1 (MQ=60)

                                                                      TATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACACTCTGG                         <  SRR4302533.19047/94‑1 (MQ=60)

                                                                       ATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACACTCTGGC                        <  SRR4302533.5208300/94‑1 (MQ=60)

                                                                       ATTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACACTCTGGC                        >  SRR4302533.3457970/1‑94 (MQ=60)

                                                                        TTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACACTCTGGCG                       <  SRR4302533.4453375/94‑1 (MQ=60)

                                                                        TTGCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACACTCTGGCG                       <  SRR4302533.1314100/94‑1 (MQ=60)

                                                                          GCCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACACTCTGGCGAT                     <  SRR4302533.3456349/94‑1 (MQ=60)

                                                                           CCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACACTCTGGCGATC                    <  SRR4302533.3399751/94‑1 (MQ=60)

                                                                           CCGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACACTCTGGCGATC                    <  SRR4302533.4325272/94‑1 (MQ=60)

                                                                            CGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACACTCTGGCGATCA                   >  SRR4302533.4330132/1‑94 (MQ=60)

                                                                            CGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACACTCTGGCGATCA                   <  SRR4302533.4247329/94‑1 (MQ=60)

                                                                            CGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACACTCTGGCGATCA                   <  SRR4302533.2740482/94‑1 (MQ=60)

                                                                            CGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACACTCTGGCGATCA                   >  SRR4302533.4163802/1‑94 (MQ=60)

                                                                            CGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACACTCTGGCGATCA                   <  SRR4302533.416008/94‑1 (MQ=60)

                                                                            CGTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACACTCTGGCGATCA                   >  SRR4302533.4036457/1‑94 (MQ=60)

                                                                             GTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACACTCTGGCGATCAG                  >  SRR4302533.5390993/1‑94 (MQ=60)

                                                                             GTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACACTCTGGCGATCAG                  >  SRR4302533.5917222/1‑94 (MQ=60)

                                                                             GTCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACACTCTGGCGATCAG                  >  SRR4302533.3735444/1‑94 (MQ=60)

                                                                              TCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACACTCTGGCGATCAGC                 <  SRR4302533.936335/94‑1 (MQ=60)

                                                                              TCAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACACTCTGGCGATCAGC                 >  SRR4302533.2864534/1‑94 (MQ=60)

                                                                               CAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACACTCTGGCGATCAGCC                >  SRR4302533.1318681/1‑94 (MQ=60)

                                                                               CAGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACACTCTGGCGATCAGCC                >  SRR4302533.4769665/1‑94 (MQ=60)

                                                                                AGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACACTCTGGCGATCAGCCT               >  SRR4302533.5920840/1‑94 (MQ=60)

                                                                                AGCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACACTCTGGCGATCAGCCT               >  SRR4302533.455568/1‑94 (MQ=60)

                                                                                 GCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACACTCTGGCGATCAGCCTT              <  SRR4302533.2860027/94‑1 (MQ=60)

                                                                                 GCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACACTCTGGCGATCAGCCTT              <  SRR4302533.4239362/94‑1 (MQ=60)

                                                                                 GCTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACACTCTGGCGATCAGCCTT              <  SRR4302533.1213062/94‑1 (MQ=60)

                                                                                  CTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACACTCTGGCGATCAGCCTTG             >  SRR4302533.135053/1‑94 (MQ=60)

                                                                                  CTGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACACTCTGGCGATCAGCCTTG             >  SRR4302533.2091398/1‑94 (MQ=60)

                                                                                   TGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACACTCTGGCGATCAGCCTTGA            <  SRR4302533.5425203/94‑1 (MQ=60)

                                                                                   TGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACACTCTGGCGATCAGCCTTGA            <  SRR4302533.1242944/94‑1 (MQ=60)

                                                                                   TGCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACACTCTGGCGATCAGCCTTGA            <  SRR4302533.1126929/94‑1 (MQ=60)

                                                                                    GCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACACTCTGGCGATCAGCCTTGAG           <  SRR4302533.5986110/94‑1 (MQ=60)

                                                                                    GCGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACACTCTGGCGATCAGCCTTGAG           <  SRR4302533.5539677/94‑1 (MQ=60)

                                                                                     CGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACACTCTGGCGATCAGCCTTGAGA          <  SRR4302533.5715218/94‑1 (MQ=60)

                                                                                     CGTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACACTCTGGCGATCAGCCTTGAGA          >  SRR4302533.3314493/1‑94 (MQ=60)

                                                                                      GTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACACTCTGGCGATCAGCCTTGAGAT         >  SRR4302533.3418582/1‑94 (MQ=60)

                                                                                      GTTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACACTCTGGCGATCAGCCTTGAGAT         >  SRR4302533.5725931/1‑94 (MQ=60)

                                                                                       TTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACACTCTGGCGATCAGCCTTGAGATA        <  SRR4302533.5772264/94‑1 (MQ=60)

                                                                                       TTCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACACTCTGGCGATCAGCCTTGAGATA        >  SRR4302533.722710/1‑94 (MQ=60)

                                                                                        TCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACACTCTGGCGATCAGCCTTGAGATAA       <  SRR4302533.6281124/94‑1 (MQ=60)

                                                                                        TCTGCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACACTCTGGCGATCAGCCTTGAGATAA       <  SRR4302533.5827923/94‑1 (MQ=60)

                                                                                           GCGCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACACTCTGGCGATCAGCCTTGAGATAATGC    <  SRR4302533.537104/94‑1 (MQ=60)

                                                                                             GCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACACTCTGGCGATCAGCCTTGAGATAATGCTG  <  SRR4302533.3109478/94‑1 (MQ=60)

                                                                                             GCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACACTCTGGCGATCAGCCTTGAGATAATGCTG  >  SRR4302533.1331084/1‑94 (MQ=60)

                                                                                             GCTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACACTCTGGCGATCAGCCTTGAGATAATGCTG  >  SRR4302533.333451/1‑94 (MQ=60)

GTCATTACTTGTTTCAGGCATGCCCGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCACTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACACTCTGGCGATCAGCCTTGAGATAATGCTG  >  NC_000913/4554684‑4554870

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`