Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,555,020 A→G M280T (ATG→ACG)  yjiE ← putative transcriptional regulator LYSR‑type

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,555,0200AG100.0% 807.0 / NA 247M280T (ATG→ACG) yjiEputative transcriptional regulator LYSR‑type
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (121/126);  total (121/126)

Only 100 of 251 total aligned reads displayed.

TGCGACGCTGGAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCATCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCTTGTTGGATGGCG  >  NC_000913/4554930‑4555109
                                                                                          |                                                                                         
tGCGACGCTGGAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTc                                                                                         >  1:1013876/1‑93 (MQ=255)
tGCGACGCTGGAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTc                                                                                         >  1:4218867/1‑93 (MQ=255)
 gCGACGCTGGAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTcc                                                                                        <  1:2946508/93‑1 (MQ=255)
 gCGACGCTGGAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTcc                                                                                        <  1:6204769/93‑1 (MQ=255)
 gCGACGCTGGAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTcc                                                                                        >  1:2027280/1‑93 (MQ=255)
   gACGCTGGAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCgg                                                                                      <  2:1889880/93‑1 (MQ=255)
   gACGCTGGAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCgg                                                                                      <  2:4467733/93‑1 (MQ=255)
     cGCTGGAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTa                                                                                    >  2:1180100/1‑93 (MQ=255)
      gcgggAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTAt                                                                                   <  2:1398897/90‑1 (MQ=255)
      gCTGGAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTAt                                                                                   <  1:1142052/93‑1 (MQ=255)
      gCTGGAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTAt                                                                                   >  1:274712/1‑93 (MQ=255)
         ggAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCa                                                                                <  2:5024595/93‑1 (MQ=255)
          gagaATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCAt                                                                               >  2:3336131/1‑93 (MQ=255)
           agaATGTCAGCTAAGCACAATCTCCAGCTGGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATa                                                                              <  1:1220083/93‑1 (MQ=255)
           agaATGTCAGCTAAGCACAATCTCCAGCTCGCTCAGTTCACGCCAGAAGCGTTCGGCATCGGGATTCATGCGGGTATTCGTCCGGTATGCATa                                                                              <  1:627252/93‑1 (MQ=255)
           agaATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATa                                                                              <  2:273204/93‑1 (MQ=255)
            gaATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAg                                                                             >  2:4353115/1‑93 (MQ=255)
            gaATGTCAGCTAAGCACAATATCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAg                                                                             <  1:1462359/93‑1 (MQ=255)
              aTGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGc                                                                           >  2:6305359/1‑93 (MQ=255)
               tGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGcc                                                                          <  2:1240510/93‑1 (MQ=255)
               tGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGcc                                                                          >  1:3223721/1‑93 (MQ=255)
               tGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGcc                                                                          >  2:2062392/1‑93 (MQ=255)
                 tCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTg                                                                        >  2:4720539/1‑93 (MQ=255)
                  cAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGa                                                                       <  1:4812917/93‑1 (MQ=255)
                  cAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGa                                                                       >  1:2270601/1‑93 (MQ=255)
                    gCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAAt                                                                     >  2:4184531/1‑93 (MQ=255)
                    gCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAAt                                                                     >  1:4525135/1‑93 (MQ=255)
                     cTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATc                                                                    <  1:1856885/93‑1 (MQ=255)
                      tAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCg                                                                   >  2:4638168/1‑93 (MQ=255)
                         gCACAATCTCCAGCTCGCGCAGGTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGa                                                                >  1:565462/1‑93 (MQ=255)
                          cacaATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGAt                                                               <  1:2799070/93‑1 (MQ=255)
                          cacaATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGAt                                                               >  1:5388441/1‑93 (MQ=255)
                           acaATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATc                                                              <  2:770982/93‑1 (MQ=255)
                            caATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCa                                                             >  2:5622542/1‑93 (MQ=255)
                               tctcCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCa                                                          >  1:368205/1‑93 (MQ=255)
                                ctcCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAg                                                         <  2:2398358/93‑1 (MQ=255)
                                  ccAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGtt                                                       <  2:5692067/93‑1 (MQ=255)
                                  ccAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGtt                                                       <  2:4746555/93‑1 (MQ=255)
                                  ccAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGtt                                                       <  2:3757039/93‑1 (MQ=255)
                                    aGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCg                                                     >  1:534141/1‑93 (MQ=255)
                                     gCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGt                                                    <  1:491538/93‑1 (MQ=255)
                                      gtCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTc                                                   <  1:6034585/92‑1 (MQ=255)
                                       tCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTcc                                                  <  2:2078606/93‑1 (MQ=255)
                                        cgcgCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTccc                                                 >  1:2866602/1‑93 (MQ=255)
                                        cgcgCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTccc                                                 >  2:5214633/1‑93 (MQ=255)
                                           gcAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGAt                                              >  2:3274858/1‑93 (MQ=255)
                                            cAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGAtt                                             >  2:3588851/1‑93 (MQ=255)
                                            cAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGAtt                                             <  1:1532258/93‑1 (MQ=255)
                                             aGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTc                                            >  2:4574586/1‑93 (MQ=255)
                                             aGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTc                                            >  1:4065484/1‑93 (MQ=255)
                                                 cACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTa                                        <  1:1642035/93‑1 (MQ=255)
                                                   cGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACa                                      >  2:1676216/1‑93 (MQ=255)
                                                    gCCAGAAGCGTTCGGGAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACaa                                     >  2:2376115/1‑93 (MQ=255)
                                                    gCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACaa                                     <  2:4908116/93‑1 (MQ=255)
                                                     ccAGAAGCGTTCGGCACCGGGATTCATGCGGGTATTCGTCGGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAAc                                    <  2:34519/93‑1 (MQ=255)
                                                     ccAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAAc                                    <  2:5820056/93‑1 (MQ=255)
                                                     ccAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAAc                                    <  1:155002/93‑1 (MQ=255)
                                                      cAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACg                                   >  2:3812569/1‑93 (MQ=255)
                                                       aGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGa                                  >  2:5146402/1‑93 (MQ=255)
                                                        gAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAg                                 <  2:5881675/93‑1 (MQ=255)
                                                        gAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAg                                 >  1:456167/1‑93 (MQ=255)
                                                        gAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAg                                 >  1:2129859/1‑93 (MQ=255)
                                                         aaGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGc                                <  2:4110151/93‑1 (MQ=255)
                                                          aGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCt                               <  1:957746/93‑1 (MQ=255)
                                                          aGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCt                               >  2:4276996/1‑93 (MQ=255)
                                                             gagcGGCAACGGGATGTATGCGTGTATTCGTCCGGTATGCATAGTCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTcc                            <  1:804498/90‑1 (MQ=255)
                                                             gTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTcc                            <  1:5545716/93‑1 (MQ=255)
                                                             gTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTcc                            <  2:3028750/93‑1 (MQ=255)
                                                             gTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTcc                            <  1:4629104/93‑1 (MQ=255)
                                                              ttCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTccc                           >  1:3493584/1‑93 (MQ=255)
                                                              ttCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTccc                           <  2:653806/93‑1 (MQ=255)
                                                                cGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGc                         <  1:5476544/93‑1 (MQ=255)
                                                                cGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGc                         <  2:6175/93‑1 (MQ=255)
                                                                cGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGc                         <  2:4440357/93‑1 (MQ=255)
                                                                 ggCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCt                        <  1:429356/93‑1 (MQ=255)
                                                                  gCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTg                       >  2:4006333/1‑93 (MQ=255)
                                                                  gCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTg                       >  1:2227755/1‑93 (MQ=255)
                                                                   cAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGc                      >  1:256686/1‑93 (MQ=255)
                                                                     aCGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGa                    >  1:4127942/1‑93 (MQ=255)
                                                                     aCGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGa                    >  1:6162769/1‑93 (MQ=255)
                                                                      cGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGaa                   >  2:2246419/1‑93 (MQ=255)
                                                                       gggATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAAt                  >  1:55938/1‑93 (MQ=255)
                                                                       gggATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAAt                  >  1:3189553/1‑93 (MQ=255)
                                                                        ggATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAAtt                 >  2:1061707/1‑93 (MQ=255)
                                                                         gATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAAttt                >  2:5951611/1‑93 (MQ=255)
                                                                            tCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGGTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCCGATTTCtt             >  1:3889942/1‑93 (MQ=255)
                                                                             cATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCttg            <  1:5055160/93‑1 (MQ=255)
                                                                              aTGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCttgt           <  1:5860375/93‑1 (MQ=255)
                                                                                gCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCttgttg         >  2:1840125/1‑93 (MQ=255)
                                                                                gCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCttgttg         <  2:1624236/93‑1 (MQ=255)
                                                                                  gggTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCTTGTTGGa       >  2:2252220/1‑93 (MQ=255)
                                                                                  gggTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCTTGTTGGa       <  1:5868507/93‑1 (MQ=255)
                                                                                   ggTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCTTTTTGGAt      >  1:5300362/1‑93 (MQ=255)
                                                                                   ggTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCTTGTTGGAt      <  1:335317/93‑1 (MQ=255)
                                                                                   ggTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCTTGTTGGAt      <  2:1323147/93‑1 (MQ=255)
                                                                                   ggTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCTTGTTGGAt      >  1:5457968/1‑93 (MQ=255)
                                                                                   ggTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCTTGTTGGAt      >  1:1442784/1‑93 (MQ=255)
                                                                                     tATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGCATTTCTTGTTGGATgg    >  1:1291297/1‑93 (MQ=255)
                                                                                     tATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCTTGTTGGATgg    >  1:3848851/1‑93 (MQ=255)
                                                                                      tttCGTCGGGTATGCATAGGCCTGAATCGCGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCTTGTTGGATGGc   <  2:4042982/92‑1 (MQ=255)
                                                                                          |                                                                                         
TGCGACGCTGGAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCATCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCTTGTTGGATGGCG  >  NC_000913/4554930‑4555109

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CAGTGCGACGCTGGAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCATCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCTTGTTGGATGGCGTACT  >  NC_000913/4554927‑4555113
                                                                                             |                                                                                             
CAGTGCGACGCTGGAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCG                                                                                               <  SRR4302533.99749/94‑1 (MQ=60)
CAGTGCGACGCTGGAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCG                                                                                               <  SRR4302533.4059240/94‑1 (MQ=60)
CAGTGCGACGCTGGAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCG                                                                                               >  SRR4302533.1742464/1‑94 (MQ=60)
 AGTGCGACGCTGGAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGT                                                                                              >  SRR4302533.6159244/1‑94 (MQ=60)
  GTGCGACGCTGGAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTC                                                                                             >  SRR4302533.1019812/1‑94 (MQ=60)
  GTGCGACGCTGGAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTC                                                                                             >  SRR4302533.4244382/1‑94 (MQ=60)
  GTGCGACGCTGGAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTC                                                                                             >  SRR4302533.1112598/1‑94 (MQ=60)
   TGCGACGCTGGAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCC                                                                                            >  SRR4302533.2039797/1‑94 (MQ=60)
    GCGACGCTGGAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCG                                                                                           <  SRR4302533.2964526/94‑1 (MQ=60)
    GCGACGCTGGAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCG                                                                                           <  SRR4302533.5346680/94‑1 (MQ=60)
    GCGACGCTGGAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCG                                                                                           <  SRR4302533.5564866/94‑1 (MQ=60)
    GCGACGCTGGAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCG                                                                                           <  SRR4302533.6241979/94‑1 (MQ=60)
     CGACGCTGGAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGG                                                                                          <  SRR4302533.3504490/94‑1 (MQ=60)
      GACGCTGGAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGT                                                                                         >  SRR4302533.1274524/1‑94 (MQ=60)
       ACGCTGGAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTA                                                                                        >  SRR4302533.4928556/1‑94 (MQ=60)
        CGCTGGAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTAT                                                                                       >  SRR4302533.1511985/1‑94 (MQ=60)
        CGCTGGAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTAT                                                                                       <  SRR4302533.5602433/94‑1 (MQ=60)
        CGCTGGAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTAT                                                                                       <  SRR4302533.885263/94‑1 (MQ=60)
        CGCTGGAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTAT                                                                                       >  SRR4302533.276383/1‑94 (MQ=60)
        CGCTGGAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTAT                                                                                       >  SRR4302533.3212815/1‑94 (MQ=60)
         GCTGGAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATG                                                                                      <  SRR4302533.1148860/94‑1 (MQ=60)
         GCTGGAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATG                                                                                      <  SRR4302533.1190337/94‑1 (MQ=60)
         GCTGGAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATG                                                                                      <  SRR4302533.5689643/94‑1 (MQ=60)
           TGGAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCA                                                                                    <  SRR4302533.4181247/94‑1 (MQ=60)
           TGGAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCA                                                                                    >  SRR4302533.1415633/1‑94 (MQ=60)
            GGAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCAT                                                                                   >  SRR4302533.513560/1‑94 (MQ=60)
            GGAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCAT                                                                                   >  SRR4302533.3895527/1‑94 (MQ=60)
             GAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATA                                                                                  <  SRR4302533.6222316/94‑1 (MQ=60)
             GAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATA                                                                                  <  SRR4302533.5746846/94‑1 (MQ=60)
             GAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATA                                                                                  <  SRR4302533.5054667/94‑1 (MQ=60)
              AGAATGTCAGCTAAGCACAATCTCCAGCTGGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAG                                                                                 <  SRR4302533.1227406/94‑1 (MQ=60)
              AGAATGTCAGCTAAGCACAATCTCCAGCTCGCTCAGTTCACGCCAGAAGCGTTCGGCATCGGGATTCATGCGGGTATTCGTCCGGTATGCATAG                                                                                 <  SRR4302533.630938/94‑1 (MQ=60)
              AGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAG                                                                                 <  SRR4302533.4260823/94‑1 (MQ=60)
              AGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAG                                                                                 <  SRR4302533.2429147/94‑1 (MQ=60)
               GAATGTCAGCTAAGCACAATATCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGG                                                                                <  SRR4302533.1471176/94‑1 (MQ=60)
                AATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGC                                                                               >  SRR4302533.2900579/1‑94 (MQ=60)
                AATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGC                                                                               >  SRR4302533.2900580/1‑94 (MQ=60)
                 ATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCC                                                                              >  SRR4302533.132703/1‑94 (MQ=60)
                 ATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCC                                                                              >  SRR4302533.1169109/1‑94 (MQ=60)
                 ATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCC                                                                              >  SRR4302533.3243599/1‑94 (MQ=60)
                    TCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGA                                                                           >  SRR4302533.2284651/1‑94 (MQ=60)
                     CAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAA                                                                          <  SRR4302533.4842085/94‑1 (MQ=60)
                     CAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAA                                                                          >  SRR4302533.3333968/1‑94 (MQ=60)
                     CAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAA                                                                          >  SRR4302533.6175417/1‑94 (MQ=60)
                      AGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAAT                                                                         >  SRR4302533.2632197/1‑94 (MQ=60)
                      AGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAAT                                                                         >  SRR4302533.5421461/1‑94 (MQ=60)
                      AGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAAT                                                                         >  SRR4302533.4552531/1‑94 (MQ=60)
                       GCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATC                                                                        <  SRR4302533.3682850/94‑1 (MQ=60)
                        CTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCG                                                                       >  SRR4302533.4337094/1‑94 (MQ=60)
                        CTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCG                                                                       <  SRR4302533.1868212/94‑1 (MQ=60)
                         TAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGG                                                                      <  SRR4302533.1911906/94‑1 (MQ=60)
                          AAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGG                                                                     <  SRR4302533.3758785/94‑1 (MQ=60)
                           AGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGA                                                                    <  SRR4302533.5412895/94‑1 (MQ=60)
                           AGCACAATCTCCAGCTCGCGCAGGTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGA                                                                    >  SRR4302533.568814/1‑94 (MQ=60)
                           AGCACAATCTCCAGCGCGCGCAGTGCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGA                                                                    <  SRR4302533.5493545/94‑1 (MQ=60)
                            GCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGAT                                                                   >  SRR4302533.5420820/1‑94 (MQ=60)
                             CACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATC                                                                  <  SRR4302533.2816286/94‑1 (MQ=60)
                              ACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCA                                                                 <  SRR4302533.4132804/94‑1 (MQ=60)
                                AATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACC                                                               <  SRR4302533.462508/94‑1 (MQ=60)
                                 ATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCA                                                              >  SRR4302533.370431/1‑94 (MQ=60)
                                  TCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAG                                                             <  SRR4302533.4041257/94‑1 (MQ=60)
                                   CTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGT                                                            <  SRR4302533.3861339/94‑1 (MQ=60)
                                     CCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTC                                                          >  SRR4302533.155822/1‑94 (MQ=60)
                                      CAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCG                                                         >  SRR4302533.537320/1‑94 (MQ=60)
                                      CAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCG                                                         >  SRR4302533.5671287/1‑94 (MQ=60)
                                       AGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGT                                                        >  SRR4302533.4061146/1‑94 (MQ=60)
                                        GCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTC                                                       <  SRR4302533.494475/94‑1 (MQ=60)
                                         GTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCC                                                      <  SRR4302533.6071157/94‑1 (MQ=60)
                                          TCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCC                                                     >  SRR4302533.2884238/1‑94 (MQ=60)
                                              GCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATT                                                 >  SRR4302533.4595135/1‑94 (MQ=60)
                                               CAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTC                                                >  SRR4302533.4090233/1‑94 (MQ=60)
                                               CAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTC                                                <  SRR4302533.2192981/94‑1 (MQ=60)
                                               CAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTC                                                <  SRR4302533.1541463/94‑1 (MQ=60)
                                                AGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCA                                               >  SRR4302533.589889/1‑94 (MQ=60)
                                                 GTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAG                                              <  SRR4302533.5299752/94‑1 (MQ=60)
                                                   TCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTA                                            >  SRR4302533.1536262/1‑94 (MQ=60)
                                                    CACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTAC                                           <  SRR4302533.1651982/94‑1 (MQ=60)
                                                       GCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAAC                                        >  SRR4302533.1001756/1‑94 (MQ=60)
                                                        CCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACG                                       <  SRR4302533.155988/94‑1 (MQ=60)
                                                         CAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGA                                      <  SRR4302533.1625162/94‑1 (MQ=60)
                                                         CAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGA                                      <  SRR4302533.1469995/94‑1 (MQ=60)
                                                          AGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAG                                     <  SRR4302533.5385637/94‑1 (MQ=60)
                                                          AGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAG                                     >  SRR4302533.2142993/1‑94 (MQ=60)
                                                          AGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAG                                     >  SRR4302533.458893/1‑94 (MQ=60)
                                                             AGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTT                                  <  SRR4302533.963378/94‑1 (MQ=60)
                                                             AGCGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTT                                  <  SRR4302533.1604995/94‑1 (MQ=60)
                                                               CGTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCC                                <  SRR4302533.2196551/94‑1 (MQ=60)
                                                                gagCGGCAACGGGATGTATGCGTGTATTCGTCCGGTATGCATAGTCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCC                               <  SRR4302533.809222/91‑1 (MQ=60)
                                                                GTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCC                               <  SRR4302533.4657159/94‑1 (MQ=60)
                                                                GTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCC                               >  SRR4302533.3515135/1‑94 (MQ=60)
                                                                GTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCC                               <  SRR4302533.5579212/94‑1 (MQ=60)
                                                                GTTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCC                               <  SRR4302533.2340162/94‑1 (MQ=60)
                                                                 TTCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCG                              <  SRR4302533.74478/94‑1 (MQ=60)
                                                                  TCGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGC                             <  SRR4302533.4200260/94‑1 (MQ=60)
                                                                   CGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCT                            <  SRR4302533.4808524/94‑1 (MQ=60)
                                                                   CGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCT                            <  SRR4302533.1697142/94‑1 (MQ=60)
                                                                   CGGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCT                            <  SRR4302533.5509557/94‑1 (MQ=60)
                                                                   CGGCAACGGAATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCT                            <  SRR4302533.5946117/94‑1 (MQ=60)
                                                                    GGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTG                           >  SRR4302533.723466/1‑94 (MQ=60)
                                                                    GGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTG                           <  SRR4302533.1894575/94‑1 (MQ=60)
                                                                    GGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTG                           >  SRR4302533.2241504/1‑94 (MQ=60)
                                                                    GGCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTG                           <  SRR4302533.431915/94‑1 (MQ=60)
                                                                     GCAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGC                          >  SRR4302533.258252/1‑94 (MQ=60)
                                                                      CAACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCG                         >  SRR4302533.6219514/1‑94 (MQ=60)
                                                                       AACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGA                        >  SRR4302533.6199823/1‑94 (MQ=60)
                                                                       AACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGA                        >  SRR4302533.2782167/1‑94 (MQ=60)
                                                                       AACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGA                        >  SRR4302533.4152997/1‑94 (MQ=60)
                                                                       AACGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGA                        >  SRR4302533.4408813/1‑94 (MQ=60)
                                                                         CGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAAT                      >  SRR4302533.4894992/1‑94 (MQ=60)
                                                                         CGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAAT                      >  SRR4302533.3209172/1‑94 (MQ=60)
                                                                         CGGGATTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAAT                      >  SRR4302533.56312/1‑94 (MQ=60)
                                                                              TTCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGGTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCCGATTTCTT                 >  SRR4302533.3913676/1‑94 (MQ=60)
                                                                               TCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCTTG                >  SRR4302533.4575507/1‑94 (MQ=60)
                                                                               TCATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCTTG                <  SRR4302533.3394709/94‑1 (MQ=60)
                                                                                CATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGTGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCTTGT               <  SRR4302533.3058476/94‑1 (MQ=60)
                                                                                CATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCTTGT               >  SRR4302533.5604147/1‑94 (MQ=60)
                                                                                CATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCTTGT               <  SRR4302533.93447/94‑1 (MQ=60)
                                                                                CATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCTTGT               <  SRR4302533.5085719/94‑1 (MQ=60)
                                                                                 ATGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCTTGTT              <  SRR4302533.5895803/94‑1 (MQ=60)
                                                                                  TGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCTTGTTG             >  SRR4302533.2866200/1‑94 (MQ=60)
                                                                                  TGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCTTGTTG             <  SRR4302533.2785748/94‑1 (MQ=60)
                                                                                  TGCGGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCTTGTTG             <  SRR4302533.549176/94‑1 (MQ=60)
                                                                                     GGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCTTTTTGGAT          >  SRR4302533.5332115/1‑94 (MQ=60)
                                                                                     GGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCTTGTTGGAT          >  SRR4302533.1451487/1‑94 (MQ=60)
                                                                                     GGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCTTGTTGGAT          <  SRR4302533.5903978/94‑1 (MQ=60)
                                                                                     GGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCTTGTTGGAT          <  SRR4302533.3213936/94‑1 (MQ=60)
                                                                                     GGGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCTTGTTGGAT          >  SRR4302533.5490849/1‑94 (MQ=60)
                                                                                      GGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCTTGTTGGATG         <  SRR4302533.337367/94‑1 (MQ=60)
                                                                                      GGTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCTTGTTGGATG         <  SRR4302533.2075109/94‑1 (MQ=60)
                                                                                       GTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGCATTTCTTGTTGGATGG        >  SRR4302533.1299077/1‑94 (MQ=60)
                                                                                       GTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCTTGTTGGATGG        >  SRR4302533.3872354/1‑94 (MQ=60)
                                                                                       GTATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCTTGTTGGATGG        <  SRR4302533.6282750/94‑1 (MQ=60)
                                                                                         ATTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCTTGTTGGATGGCG      >  SRR4302533.2258155/1‑94 (MQ=60)
                                                                                          TTCGTCCGGTATGCATAGGCCTGAATCGTGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCTTGTTGGATGGCGT     <  SRR4302533.6019448/94‑1 (MQ=60)
                                                                                          TTCGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCTTGTTGGATGGCGT     >  SRR4302533.5921045/1‑94 (MQ=60)
                                                                                            CGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCTTGTTGGATGGCGTAC   >  SRR4302533.4988924/1‑94 (MQ=60)
                                                                                            CGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCTTGTTGGATGGCGTAC   >  SRR4302533.1384236/1‑94 (MQ=60)
                                                                                            CGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCTTGTTGGATGGCGTAC   >  SRR4302533.5546608/1‑94 (MQ=60)
                                                                                            CGTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCTTGTTGGATGGCGTAC   <  SRR4302533.965017/94‑1 (MQ=60)
                                                                                             GTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCTTGTTGtagggggtgtt  >  SRR4302533.5379762/1‑83 (MQ=60)
                                                                                             GTCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCTTGTTGGATGGCGTACT  <  SRR4302533.6046014/94‑1 (MQ=60)
                                                                                             |                                                                                             
CAGTGCGACGCTGGAGAATGTCAGCTAAGCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACGGGATTCATGCGGGTATTCATCCGGTATGCATAGGCCTGAATCGGGATCACCAGTTCGTCCCGATTCAGTACAACGAGCTTCCCGCTGCGAATTTCTTGTTGGATGGCGTACT  >  NC_000913/4554927‑4555113

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑