Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,601,614 (A)7→6 coding (569/726 nt) yjjQ → putative regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,601,6080A.100.0% 1178.8 / NA 253coding (563/726 nt)yjjQputative regulator
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (135/118);  total (135/118)

Only 100 of 287 total aligned reads displayed.

AGAGCGCAAGGTACTGCGGCTATTAGGTAAAGGATGGGGCATCAACCAGATAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCCAAAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATGGATAAATAGCGCGCAGGGTGCAAGAGAACTT  >  NC_000913/4601516‑4601696
                                                                                            |                                                                                        
agagCGCAAGGTACTGCGGCTATTAGGTAAAGGATGGGGCATCAACCAGATAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCCa                                                                                          >  1:3282616/1‑93 (MQ=255)
    cgcAAGGTACTGCGGCTATTAGGTAAAGGATGGGGCATCAACCAGATAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCCaaaaa                                                                                      <  1:2281434/93‑1 (MQ=255)
     gcAAGGTACTGCGGCTATTAGGTAAAGGATGGGGCATCAACCAGATAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCCaaaaaa                                                                                     <  2:2751798/93‑1 (MQ=255)
     gcAAGGTACTGCCGCTATTAGGTAAAGGATGGGGCATCAACCAGATAGCTTCATTGCTGAAGAAAAGTAATAAAACTATCAGCGCCCaaaaaa                                                                                     <  1:5114505/93‑1 (MQ=255)
       aaGGTACTGCGGCTATTAGGTAAAGGATGGGGCATCAACCAGATAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCCaaaaaaca                                                                                   >  2:687508/1‑91 (MQ=255)
        aGGTACTGCGGCTATTAGGTAAAGGATGGGGCATCAACCAGATAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCCaaaaaacag                                                                                  <  1:5191196/93‑4 (MQ=255)
        aGGTACTGCGGCTATTAGGTAAAGGATGGGGCATCAACCAGATAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCCaaaaaacag                                                                                  >  2:6078733/1‑90 (MQ=255)
         ggTACTGCGGCTATTAGGTAAAGGATGGGGCATCAACCAGATAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCCaaaaaacagt                                                                                 <  2:944584/93‑5 (MQ=255)
         ggTACTGCGGCTATTAGGTAAAGGATGGGGCATCAACCAGATAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCCaaaaaacagt                                                                                 <  1:4079273/93‑5 (MQ=255)
          gTACTGCGGCTATTAGGTAAAGGATGGGGCATCAACCAGATAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTg                                                                               <  2:2797946/93‑1 (MQ=255)
          gTACTGCGGCTATTAGGTAAAGGATGGGGCATCAACCAGATAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTg                                                                               <  2:2580622/93‑1 (MQ=255)
          gTACTGCGGCTATTAGGTAAAGGATGGGGCATCAACCAGATAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTg                                                                               <  1:4513754/93‑1 (MQ=255)
           tACTGCGGCTATTAGGTAAAGGATGGGGCATCAACCAGATAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGc                                                                              <  1:3219698/93‑1 (MQ=255)
            aCTGCGGCTATTAGGTAAAGGATGGGGCATCAACCAGATAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCg                                                                             <  2:6195088/93‑1 (MQ=255)
            aCTGCGGCTATTAGGTAAAGGATGGGGCATCAACCAGATAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCg                                                                             >  1:2693109/1‑93 (MQ=255)
              tGCGGCTATTAGGTAAAGGATGGGGCATCAACCAGATAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGAt                                                                           >  1:3349590/1‑93 (MQ=255)
                cGGCTATTAGGTAAAGGATGGGGCATCAACCAGATAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGc                                                                         >  1:2611256/1‑93 (MQ=255)
                cGGCTATTAGGTAAAGGATGGGGCATCAACCAGATAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGc                                                                         >  2:3648035/1‑93 (MQ=255)
                cGGCTATTAGGTAAAGGATGGGGCATCAACCAGATAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGc                                                                         >  2:5708222/1‑93 (MQ=255)
                cGGCTATTAGGTAAAGGATGGGGCATCAACCAGATAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGc                                                                         <  1:4190879/93‑1 (MQ=255)
                    tATTAGGTAAAGGATGGGGCATCAACCAGATAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCg                                                                     >  1:211928/1‑93 (MQ=255)
                    tATTAGGTAAAGGATGGGGCATCAACCAGATAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCg                                                                     >  2:5120113/1‑93 (MQ=255)
                      ttAGGTAAAGGATGGGGCATCAACCAGATAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGAc                                                                   <  2:4410752/93‑1 (MQ=255)
                      ttAGGTAAAGGATGGGGCATCAACCAGATAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGAc                                                                   <  2:1950373/93‑1 (MQ=255)
                         ggTAAAGGATGGGGCATCAACCAGATAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTgg                                                                >  2:3047492/1‑93 (MQ=255)
                          gTAAAGGATGGGGCATCAACCAGATAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGc                                                               >  1:3441179/1‑93 (MQ=255)
                           tAAAGGATGGGGCATCAACCAGATAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCa                                                              <  2:2420405/93‑1 (MQ=255)
                            aaaGGATGGGGCATCAACCATATAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCaa                                                             >  2:5139879/1‑93 (MQ=255)
                              aGGATGGGGCATCAACCAGATAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAAtt                                                           <  1:4888672/93‑1 (MQ=255)
                              aGGATGGGGCATCAACCAGATAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGGGCGATGCGTCGACTGGCAAtt                                                           >  2:5966225/1‑93 (MQ=255)
                               ggATGGGGCATCAACCAGATAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTc                                                          >  2:5535010/1‑93 (MQ=255)
                                gATGGGGCATCAACCAGATAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTca                                                         <  1:3581457/93‑1 (MQ=255)
                                 aTGGGGCATCAACCAGATAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTcac                                                        <  2:1390917/93‑1 (MQ=255)
                                  tGGGGCATCAACCAGATAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTcaca                                                       >  1:4797714/1‑93 (MQ=255)
                                  tGGGGCATCAACCAGATAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTcaca                                                       >  1:619977/1‑93 (MQ=255)
                                      gCATCAACCAGATAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCaa                                                   <  1:1727491/93‑1 (MQ=255)
                                      gCATCAACCAGATAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCaa                                                   >  2:2519114/1‑93 (MQ=255)
                                      gCATCAACCAGATAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCaa                                                   <  1:772301/93‑1 (MQ=255)
                                      gCATCAACCAGATAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCaa                                                   <  1:2840439/93‑1 (MQ=255)
                                      gCATCAACCAGATAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCaa                                                   <  1:491086/93‑1 (MQ=255)
                                       cATCAACCAGATAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAAc                                                  <  2:764104/93‑1 (MQ=255)
                                         tCAACCAGATAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGc                                                <  2:1771260/93‑1 (MQ=255)
                                         tCAACCAGATAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGc                                                <  1:6114557/93‑1 (MQ=255)
                                           aaCCAGATAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTg                                              >  1:4720782/1‑93 (MQ=255)
                                            aCCAGATAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGa                                             <  1:5326984/93‑1 (MQ=255)
                                              cAGATAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGaaa                                           >  2:3568676/1‑93 (MQ=255)
                                              cAGATAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGaaa                                           <  2:4768346/93‑1 (MQ=255)
                                              cAGATAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGaaa                                           <  1:1280031/93‑1 (MQ=255)
                                              cAGATAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGaaa                                           <  1:2987170/93‑1 (MQ=255)
                                               aGATAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAAt                                          <  2:6287003/93‑1 (MQ=255)
                                                 aTAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGt                                        >  2:4040646/1‑93 (MQ=255)
                                                 aTAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGt                                        <  1:1520244/93‑1 (MQ=255)
                                                  tAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTa                                       <  1:5583993/93‑1 (MQ=255)
                                                   aGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTAt                                      <  2:3419972/93‑1 (MQ=255)
                                                      ttCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCa                                   >  2:2299274/1‑93 (MQ=255)
                                                      ttCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCa                                   >  1:1215453/1‑93 (MQ=255)
                                                      ttCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCa                                   >  1:5500412/1‑93 (MQ=255)
                                                      ttCATTGCTTAAGAAAAGGAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGTCAATTCACAGCAACGCTGAAATGTATGCa                                   >  1:1563620/1‑93 (MQ=255)
                                                       tCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCAt                                  <  1:4953204/93‑1 (MQ=255)
                                                        cATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATg                                 >  2:857609/1‑93 (MQ=255)
                                                         aTTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATgg                                <  1:1852808/93‑1 (MQ=255)
                                                          ttGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGGGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATGGa                               >  1:86683/1‑93 (MQ=255)
                                                          ttGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATGGa                               <  2:5782190/93‑1 (MQ=255)
                                                            gCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATGGATa                             <  1:6016379/93‑1 (MQ=255)
                                                            gCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATGGATa                             >  2:2957394/1‑93 (MQ=255)
                                                              ttAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATGGATaaa                           >  1:5657457/1‑93 (MQ=255)
                                                              ttAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATGGATaaa                           >  2:5609784/1‑93 (MQ=255)
                                                              ttAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATGGATaaa                           <  2:3951827/93‑1 (MQ=255)
                                                                aaGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATGGATAAATa                         <  1:952286/93‑1 (MQ=255)
                                                                aaGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATGGATAAATa                         >  2:3474063/1‑93 (MQ=255)
                                                                aaGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATGGATAAATa                         >  1:5745467/1‑93 (MQ=255)
                                                                 aGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATGGATAAATAg                        >  2:5251439/1‑93 (MQ=255)
                                                                 aGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATGGATAAATAg                        >  2:2223285/1‑93 (MQ=255)
                                                                 aGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATGGATAAATAg                        <  2:4550907/93‑1 (MQ=255)
                                                                 aGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGAATGGCAATTCACAGCAACGCTGAAATGTATGCATGGATAAATAg                        >  2:6215624/1‑93 (MQ=255)
                                                                  gAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATGGATAAATAgc                       >  2:3844335/1‑93 (MQ=255)
                                                                   aaaaGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATGGATAAATAgcg                      <  2:1620802/93‑1 (MQ=255)
                                                                    aaaGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATGGATAAATAgcgc                     <  1:3846878/93‑1 (MQ=255)
                                                                     aaGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATGGATAAATAgcgcg                    >  2:3098823/1‑93 (MQ=255)
                                                                      aGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATGGATCAATAgcgcgc                   >  1:2934253/1‑93 (MQ=255)
                                                                       gTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATGGATAAATAGCGCGCa                  <  2:5035180/93‑1 (MQ=255)
                                                                        taataaAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATGGATAAATAGCGCGCAg                 <  2:1097505/93‑1 (MQ=255)
                                                                        taataaAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATGGATAAATAGCGCGCAg                 >  1:1143215/1‑93 (MQ=255)
                                                                          ataaAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATGGATAAATAGCGCGCAggg               <  1:5609487/93‑1 (MQ=255)
                                                                           taaAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGACATGTATGCATGGATAAATAGCGCGCAgggg              >  1:4357181/1‑92 (MQ=255)
                                                                            aaaaCTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATGGATAAATAGCGCGCAGGGTg             <  2:5220112/93‑1 (MQ=255)
                                                                             aaaCTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATGGATAAATAGCGCGCTGGGTGc            <  2:2893417/93‑1 (MQ=255)
                                                                             aaaCTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATGGATAAATAGCGCGCAGGGTGc            >  2:4225065/1‑93 (MQ=255)
                                                                                cTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATGGATAAATAGCGCGCAGGGTGCAag         >  2:327473/1‑93 (MQ=255)
                                                                                cTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATGGATAAATAGCGCGCAGGGTGCAag         >  2:1245572/1‑93 (MQ=255)
                                                                                 tATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATGGATAAATAGCGCGCAGGGTGCAaga        <  1:2219909/93‑1 (MQ=255)
                                                                                 tATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATGGATAAATAGCGCGCAGGGTGCAaga        >  1:983997/1‑93 (MQ=255)
                                                                                 tATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATGGATAAATAGCGCGCAGGGTGCAaga        <  2:2641586/93‑1 (MQ=255)
                                                                                 tATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATGGATAAATAGCGCGCAGGGTGCAaga        >  2:1787515/1‑93 (MQ=255)
                                                                                  aTCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATGGCTAAATAGCGCGCAGGGTGCAagag       >  2:6182783/1‑93 (MQ=255)
                                                                                  aTCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATGGATAAATAGCGCGCAGGGTGCAagag       <  1:2795022/93‑1 (MQ=255)
                                                                                  aTCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATGGATAAATAGCGCGCAGGGTGCAagag       <  2:2825693/93‑1 (MQ=255)
                                                                                   tCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATGGATAAATAGCGCGCAGGGTGCAAgaga      >  1:5861408/1‑93 (MQ=255)
                                                                                      gcgcCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATGGATAAATAGCGCGCAGGGTGCAAGAGaact   >  1:3014873/1‑93 (MQ=255)
                                                                                      gcgcCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATGGATAAATAGCGCGCAGGGTGCAAGAGaact   <  1:2371425/93‑1 (MQ=255)
                                                                                            |                                                                                        
AGAGCGCAAGGTACTGCGGCTATTAGGTAAAGGATGGGGCATCAACCAGATAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCCAAAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATGGATAAATAGCGCGCAGGGTGCAAGAGAACTT  >  NC_000913/4601516‑4601696

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCAAGGTACTGCGGCTATTAGGTAAAGGATGGGGCATCAACCAGATAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCCAAAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATGGATAAATAGCGCGCAGGGTGCAAGAGAACTTAACTTGCCTTC  >  NC_000913/4601521‑4601707
                                                                                             |                                                                                             
GCAAGGTACTGCGGCTATTAGGTAAAGGATGGGGCATCAACCAGATAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCCAAAAAAC                                                                                               <  SRR4302533.6150682/94‑1 (MQ=60)
GCAAGGTACTGCCGCTATTAGGTAAAGGATGGGGCATCAACCAGATAGCTTCATTGCTGAAGAAAAGTAATAAAACTATCAGCGCCCAAAAAAC                                                                                               <  SRR4302533.5145415/94‑1 (MQ=60)
  AAGGTACTGCGGCTATTAGGTAAAGGATGGGGCATCAACCAGATAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAG                                                                                            >  SRR4302533.2793459/1‑94 (MQ=60)
  AAGGTACTGCGGCTATTAGGTAAAGGATGGGGCATCAACCAGATAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAG                                                                                            >  SRR4302533.1276728/1‑94 (MQ=60)
   AGGTACTGCGGCTATTAGGTAAAGGATGGGGCATCAACCAGATAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGT                                                                                           <  SRR4302533.5222513/94‑1 (MQ=60)
   AGGTACTGCGGCTATTAGGTAAAGGATGGGGCATCAACCAGATAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGT                                                                                           <  SRR4302533.5260376/94‑1 (MQ=60)
   AGGTACTGCGGCTATTAGGTAAAGGATGGGGCATCAACCAGATAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGT                                                                                           >  SRR4302533.966970/1‑94 (MQ=60)
   AGGTACTGCGGCTATTAGGTAAAGGATGGGGCATCAACCAGATAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGT                                                                                           <  SRR4302533.4886778/94‑1 (MQ=60)
    GGTACTGCGGCTATTAGGTAAAGGATGGGGCATCAACCAGATAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTG                                                                                          <  SRR4302533.4104106/94‑1 (MQ=60)
     GTACTGCGGCTATTAGGTAAAGGATGGGGCATCAACCAGATAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGC                                                                                         <  SRR4302533.5684772/94‑1 (MQ=60)
     GTACTGCGGCTATTAGGTAAAGGATGGGGCATCAACCAGATAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGC                                                                                         <  SRR4302533.4541088/94‑1 (MQ=60)
      TACTGCGGCTATTAGGTAAAGGATGGGGCATCAACCAGATAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCG                                                                                        <  SRR4302533.3239550/94‑1 (MQ=60)
      TACTGCGGCTATTAGGTAAAGGATGGGGCATCAACCAGATAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCG                                                                                        >  SRR4302533.2709611/1‑94 (MQ=60)
        CTGCGGCTATTAGGTAAAGGATGGGGCATCAACCAGATAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGAT                                                                                      >  SRR4302533.3370283/1‑94 (MQ=60)
        ATGCGGCTATTAGGTAAAGGATGGGGCATCAACCAGATAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGAT                                                                                      <  SRR4302533.5806338/94‑1 (MQ=60)
         TGCGGCTATTAGGTAAAGGATGGGGCATCAACCAGATAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATG                                                                                     <  SRR4302533.5818140/94‑1 (MQ=60)
         TGCGGCTATTAGGTAAAGGATGGGGCATCAACCAGATAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATG                                                                                     <  SRR4302533.6039652/94‑1 (MQ=60)
         TGCGGCTATTAGGTAAAGGATGGGGCATCAACCAGATAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATG                                                                                     <  SRR4302533.1243293/94‑1 (MQ=60)
         TGCGGCTATTAGGTAAAGGATGGGGCATCAACCAGATAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATG                                                                                     >  SRR4302533.2939946/1‑94 (MQ=60)
          GCGGCTATTAGGTAAAGGATGGGGCATCAACCAGATAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGC                                                                                    >  SRR4302533.2627282/1‑94 (MQ=60)
           CGGCTATTAGGTAAAGGATGGGGCATCAACCAGATAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCG                                                                                   <  SRR4302533.4942183/94‑1 (MQ=60)
           CGGCTATTAGGTAAAGGATGGGGCATCAACCAGATAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCG                                                                                   <  SRR4302533.4216245/94‑1 (MQ=60)
           CGGCTATTAGGTAAAGGATGGGACATCAACCAGATAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCG                                                                                   >  SRR4302533.4891138/1‑94 (MQ=60)
              CTATTAGGTAAAGGATGGGGCATCAACCAGATAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCG                                                                                >  SRR4302533.213264/1‑94 (MQ=60)
                ATCAGGTAAAGGATGGGGCATCAACCAGATAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGACC‑AAAAAACAGTGCGATGCGTCCAC                                                                              >  SRR4302533.790969/1‑94 (MQ=60)
                   AGGTAAAGGATGGGGCATCAACCAGATAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGG                                                                           <  SRR4302533.5181474/94‑1 (MQ=60)
                    GGTAAAGGATGGGGCATCAACCAGATAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGC                                                                          >  SRR4302533.3462427/1‑94 (MQ=60)
                    GGTAAAGGATGGGGCATCAACCAGATAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGC                                                                          <  SRR4302533.1968912/94‑1 (MQ=60)
                       AAAGGATGGGGCATCAACCAGATAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAAT                                                                       <  SRR4302533.4753055/94‑1 (MQ=60)
                         AGGATGGGGCATCAACCAGATAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTC                                                                     <  SRR4302533.87590/94‑1 (MQ=60)
                         AGGATGGGGCATCAACCAGATAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTC                                                                     <  SRR4302533.4918293/94‑1 (MQ=60)
                         AGGATGGGGCATCAACCAGATAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTC                                                                     <  SRR4302533.2650547/94‑1 (MQ=60)
                         AGGATGGGGCATCAACCAGATAGCTTCATTGCTTAAGAAAAGAAATAAAACTATCAGCGCCC‑AAACAACAGTGCGATGCGTCGACTGGAAATTC                                                                     >  SRR4302533.576907/1‑94 (MQ=60)
                          GGATGGGGCATCATCCAGATAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCA                                                                    <  SRR4302533.3518825/94‑1 (MQ=60)
                          GGATGGGGCATCAACCAGATAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCA                                                                    >  SRR4302533.5600026/1‑94 (MQ=60)
                           GATGGGGCATCAACCAGATAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCAC                                                                   <  SRR4302533.3603519/94‑1 (MQ=60)
                            ATGGGGCATCAGCCAGATAGCTTCATTGCTTAAGAAAAGTAATAAACCTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACA                                                                  <  SRR4302533.1389326/94‑1 (MQ=60)
                            ATGGGGCATCAACCAGATAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACA                                                                  >  SRR4302533.4826775/1‑94 (MQ=60)
                            ATGGGGCATCAACCAGATAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACA                                                                  >  SRR4302533.623617/1‑94 (MQ=60)
                               GGGCATCAACCAGATAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCA                                                               >  SRR4302533.2163412/1‑94 (MQ=60)
                                 GCATCAACCAGATAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAAC                                                             <  SRR4302533.494020/94‑1 (MQ=60)
                                 GCATCAACCAGATAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAAC                                                             <  SRR4302533.1737973/94‑1 (MQ=60)
                                 GCATCAACCAGATAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAAC                                                             <  SRR4302533.776844/94‑1 (MQ=60)
                                 GCATCAACCAGATAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAAC                                                             <  SRR4302533.2857920/94‑1 (MQ=60)
                                  CATCAACCAGATAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACG                                                            >  SRR4302533.6198285/1‑94 (MQ=60)
                                   ATCAACCAGATAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGC                                                           >  SRR4302533.3379085/1‑94 (MQ=60)
                                   ATCAACCAGATAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGC                                                           >  SRR4302533.6133052/1‑94 (MQ=60)
                                    TCAACCAGATAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCT                                                          <  SRR4302533.2288201/94‑1 (MQ=60)
                                    TCAACCAGATAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCT                                                          <  SRR4302533.6151452/94‑1 (MQ=60)
                                     CAACCAGATAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTG                                                         >  SRR4302533.4749405/1‑94 (MQ=60)
                                     CAACCAGATAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTG                                                         >  SRR4302533.3251951/1‑94 (MQ=60)
                                     CAACCAGATAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTG                                                         <  SRR4302533.2068330/94‑1 (MQ=60)
                                      AACCAGATAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGA                                                        >  SRR4302533.2977952/1‑94 (MQ=60)
                                       ACCAGATAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAA                                                       >  SRR4302533.2844307/1‑94 (MQ=60)
                                       ACCAGATAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAA                                                       >  SRR4302533.5318562/1‑94 (MQ=60)
                                       ACCAGATAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAA                                                       <  SRR4302533.5358907/94‑1 (MQ=60)
                                         CAGATAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAAT                                                     <  SRR4302533.3005443/94‑1 (MQ=60)
                                         CAGATAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAAT                                                     <  SRR4302533.1287726/94‑1 (MQ=60)
                                         CAGATAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAAT                                                     <  SRR4302533.4027405/94‑1 (MQ=60)
                                         CAGATAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAAT                                                     >  SRR4302533.4395807/1‑94 (MQ=60)
                                           GATAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGT                                                   >  SRR4302533.5630370/1‑94 (MQ=60)
                                           GATAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGT                                                   <  SRR4302533.2683617/94‑1 (MQ=60)
                                            ATAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTA                                                  <  SRR4302533.1529397/94‑1 (MQ=60)
                                             TAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTAT                                                 >  SRR4302533.2311615/1‑94 (MQ=60)
                                             TAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTAT                                                 <  SRR4302533.5617733/94‑1 (MQ=60)
                                              AGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATG                                                <  SRR4302533.564114/94‑1 (MQ=60)
                                              AGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATG                                                >  SRR4302533.4922175/1‑94 (MQ=60)
                                              AGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATG                                                >  SRR4302533.2747148/1‑94 (MQ=60)
                                              AGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATG                                                >  SRR4302533.63090/1‑94 (MQ=60)
                                               GCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGC                                               >  SRR4302533.3303167/1‑94 (MQ=60)
                                               GCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGC                                               <  SRR4302533.1187911/94‑1 (MQ=60)
                                                CTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCA                                              >  SRR4302533.1222751/1‑94 (MQ=60)
                                                CTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCA                                              >  SRR4302533.41995/1‑94 (MQ=60)
                                                CTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCA                                              >  SRR4302533.5533593/1‑94 (MQ=60)
                                                CTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCA                                              >  SRR4302533.1563606/1‑94 (MQ=60)
                                                CTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCA                                              >  SRR4302533.1850895/1‑94 (MQ=60)
                                                CTTCATTGCTTAAGAAAAGGAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGTCAATTCACAGCAACGCTGAAATGTATGCA                                              >  SRR4302533.1573031/1‑94 (MQ=60)
                                                 TTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCAT                                             >  SRR4302533.986054/1‑94 (MQ=60)
                                                 TTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCAT                                             >  SRR4302533.2840431/1‑94 (MQ=60)
                                                 TTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCAT                                             >  SRR4302533.5608276/1‑94 (MQ=60)
                                                 TTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCAT                                             <  SRR4302533.3007563/94‑1 (MQ=60)
                                                  TCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATG                                            <  SRR4302533.4983205/94‑1 (MQ=60)
                                                   CATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATGG                                           >  SRR4302533.1293216/1‑94 (MQ=60)
                                                   CATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATGG                                           <  SRR4302533.4418591/94‑1 (MQ=60)
                                                    ATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGGGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATGGA                                          >  SRR4302533.87241/1‑94 (MQ=60)
                                                    ATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATGGA                                          >  SRR4302533.4798599/1‑94 (MQ=60)
                                                    ATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATGGA                                          <  SRR4302533.1864106/94‑1 (MQ=60)
                                                      TGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATGGATA                                        <  SRR4302533.3198378/94‑1 (MQ=60)
                                                      TGCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATGGATA                                        <  SRR4302533.4955078/94‑1 (MQ=60)
                                                       GCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATGGATAA                                       <  SRR4302533.6052835/94‑1 (MQ=60)
                                                       GCTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATGGATAA                                       >  SRR4302533.3516160/1‑94 (MQ=60)
                                                        CTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATGGATAAA                                      >  SRR4302533.2494976/1‑94 (MQ=60)
                                                        CTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATGGATAAA                                      >  SRR4302533.5691642/1‑94 (MQ=60)
                                                        CTTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATGGATAAA                                      >  SRR4302533.161012/1‑94 (MQ=60)
                                                         TTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATGGATAAAT                                     >  SRR4302533.3269106/1‑94 (MQ=60)
                                                         TTAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATGGATAAAT                                     <  SRR4302533.1854496/94‑1 (MQ=60)
                                                          TAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATGGATAAATA                                    >  SRR4302533.5780137/1‑94 (MQ=60)
                                                          TAAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATGGATAAATA                                    >  SRR4302533.1003182/1‑94 (MQ=60)
                                                           AAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATGGATAAATAG                                   <  SRR4302533.5412239/94‑1 (MQ=60)
                                                           AAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATGGATAAATAG                                   <  SRR4302533.342432/94‑1 (MQ=60)
                                                           AAGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATGGATAAATAG                                   <  SRR4302533.957880/94‑1 (MQ=60)
                                                            AGAAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATGGATAAATAGC                                  >  SRR4302533.5108473/1‑94 (MQ=60)
                                                              AAAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATGGATAAATAGCGC                                >  SRR4302533.3855325/1‑94 (MQ=60)
                                                               AAAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATGGATAAATAGCGCG                               <  SRR4302533.3870369/94‑1 (MQ=60)
                                                                AAGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATGGATCAATAGCGCGC                              >  SRR4302533.2952194/1‑94 (MQ=60)
                                                                 AGTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATGGATAAATAGCGCGCA                             <  SRR4302533.4698464/94‑1 (MQ=60)
                                                                  GTAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATGGATAAATAGCGCGCAG                            >  SRR4302533.1150033/1‑94 (MQ=60)
                                                                  ATAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATGGATAAATAGCGCGCAG                            <  SRR4302533.1561932/94‑1 (MQ=60)
                                                                   TAATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATGGATAAATAGCGCGCAGG                           <  SRR4302533.4222530/94‑1 (MQ=60)
                                                                    AATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATGGATAAATAGCGCGCAGGG                          >  SRR4302533.5213985/1‑94 (MQ=60)
                                                                     ATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGACATGTATGCATGGATAAATAGCGCGCAGGGG                         >  SRR4302533.4383481/1‑94 (MQ=60)
                                                                     ATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATGGATAAATAGCGCGCAGGGT                         >  SRR4302533.5515725/1‑94 (MQ=60)
                                                                     ATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATGGATAAATAGCGCGCAGGGT                         >  SRR4302533.3197125/1‑94 (MQ=60)
                                                                     ATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATGGATAAATAGCGCGCAGGGT                         <  SRR4302533.4589769/94‑1 (MQ=60)
                                                                     ATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATGGATAAATAGCGCGCAGGGT                         <  SRR4302533.5643387/94‑1 (MQ=60)
                                                                     ATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATGGATAAATAGCGCGCAGGGG                         >  SRR4302533.3012461/1‑94 (MQ=60)
                                                                     ATAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATGGATAAATAGCGCGCAGGGG                         >  SRR4302533.3740428/1‑94 (MQ=60)
                                                                      TAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATGGATAAATAGCGCGCAGGGTG                        <  SRR4302533.1645253/94‑1 (MQ=60)
                                                                      TAAAACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATGGATAAATAGCGCGCAGGGTG                        >  SRR4302533.5699306/1‑94 (MQ=60)
                                                                          ACTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATGGATAAATAGCGCGCAGGGTGCAAG                    <  SRR4302533.829449/94‑1 (MQ=60)
                                                                           CTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATGGATAAATAGCGCGCAGGGTGCAAGA                   >  SRR4302533.3320908/1‑94 (MQ=60)
                                                                           CTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATGGATAAATAGCGCGCAGGGTGCAAGA                   >  SRR4302533.989765/1‑94 (MQ=60)
                                                                           CTATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATGGATAAATAGCGCGCAGGGTGCAAGA                   >  SRR4302533.6026098/1‑94 (MQ=60)
                                                                            TATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATGGATAAATAGCGCGCAGGGTGCAAGAG                  <  SRR4302533.2233612/94‑1 (MQ=60)
                                                                            TATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATGGATAAATAGCGCGCAGGGTGCAAGAG                  >  SRR4302533.75423/1‑94 (MQ=60)
                                                                             ATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATGGATAAATAGCGCGCAGGGTGCAAGAGA                 <  SRR4302533.2322262/94‑1 (MQ=60)
                                                                             ATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATGGATAAATAGCGCGCAGGGTGCAAGAGA                 >  SRR4302533.5896848/1‑94 (MQ=60)
                                                                             ATCAGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATGGATAAATAGCGCGCAGGGTGCAAGAGA                 <  SRR4302533.2812211/94‑1 (MQ=60)
                                                                                AGCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATGGATAAATAGCGCGCAGGGTGCAAGAGAACT              >  SRR4302533.3033310/1‑94 (MQ=60)
                                                                                 GCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATGGATAAATAGCGCGCAGGGTGCAAGAGAACTT             >  SRR4302533.4998415/1‑94 (MQ=60)
                                                                                 GCGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATGGATAAATAGCGCGCAGGGTGCAAGAGAACTT             <  SRR4302533.2386098/94‑1 (MQ=60)
                                                                                  CGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATGGATAAATAGCGCGCAGGGTGCAAGAGAACTTA            >  SRR4302533.3801653/1‑94 (MQ=60)
                                                                                  CGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATGGATAAATAGCGCGCAGGGTGCAAGAGAACTTA            >  SRR4302533.482345/1‑94 (MQ=60)
                                                                                  CGCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATGGATAAATAGCGCGCAGGGTGCAAGAGAACTTA            >  SRR4302533.3340852/1‑94 (MQ=60)
                                                                                   GCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATGGATAAATAGCGCGCAGGGTGCAAGAGAACTTAA           >  SRR4302533.3281977/1‑94 (MQ=60)
                                                                                   GCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATGGATAAATAGCGCGCAGGGTGCAAGAGAACTTAA           >  SRR4302533.5309135/1‑94 (MQ=60)
                                                                                   GCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATGGATAAATAGCGCGCAGGGTGCAAGAGAACTTAA           <  SRR4302533.4506651/94‑1 (MQ=60)
                                                                                   GCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATGGATAAATAGCGCGCAGGGTGCAAGAGAACTTAA           >  SRR4302533.4031881/1‑94 (MQ=60)
                                                                                   GCCC‑AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATGGATAAATAGCGCGCAGGGTGCAAGAGAACTTAA           >  SRR4302533.336402/1‑94 (MQ=60)
                                                                                     CCCAAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATGGATAAATAGCGCGCAGGGTGCAAGAGAACTTAAC          >  SRR4302533.5501651/1‑94 (MQ=60)
                                                                                     CCCAAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATGGATAAATAGCGCGCAGGGTGCAAGAGAACTTAAC          <  SRR4302533.86586/94‑1 (MQ=60)
                                                                                      CCAAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATGGATAAATAGCGCGCAGGGTGCAAGAGAACTTAACT         >  SRR4302533.1267738/1‑94 (MQ=60)
                                                                                      CCAAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATGGATAAATAGCGCGCAGGGTGCAAGAGAACTTAACT         <  SRR4302533.2018310/94‑1 (MQ=60)
                                                                                       CAAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATGGATAAATAGCGCGCAGGGTGCAAGAGAACTTAACTT        <  SRR4302533.623720/94‑1 (MQ=60)
                                                                                        AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATGGATAAATAGCGCGCAGGGTGCAAGAGAACTTAACTTG       <  SRR4302533.6115498/94‑1 (MQ=60)
                                                                                        AAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATGGATAAATAGCGCGCAGGGTGCAAGAGAACTTAACTTG       <  SRR4302533.4802568/94‑1 (MQ=60)
                                                                                         AAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATGGATAAATAGCGCGCAGGGTGCAAGAGAACTTAACTTGC      >  SRR4302533.4738066/1‑94 (MQ=60)
                                                                                         AAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATGGATAAATAGCGCGCAGGGTGCAAGAGAACTTAACTTGC      <  SRR4302533.3290243/94‑1 (MQ=60)
                                                                                          AAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATGGATAAATAGCGCGCAGGGTGCAAGAGAACTTAACTTGCC     <  SRR4302533.6215338/94‑1 (MQ=60)
                                                                                           AAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATGGATAAATAGCGCGCAGGGTGCAAGAGAACTTAACTTGCCT    <  SRR4302533.3946580/94‑1 (MQ=60)
                                                                                             ACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATGGATAAATAGCGCGCAGGGTGCAAGAGAACTTAACTTGCCTTC  <  SRR4302533.181106/94‑1 (MQ=60)
                                                                                             ACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATGGATAAATAGCGCGCAGGGTGCAAGAGAACTTAACTTGCCTTC  <  SRR4302533.6219289/94‑1 (MQ=60)
                                                                                             ACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATGGATAAATAGCGCGCAGGGTGCAAGAGAACTTAACTTGCCTTC  >  SRR4302533.3461026/1‑94 (MQ=60)
                                                                                             |                                                                                             
GCAAGGTACTGCGGCTATTAGGTAAAGGATGGGGCATCAACCAGATAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCCAAAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATGGATAAATAGCGCGCAGGGTGCAAGAGAACTTAACTTGCCTTC  >  NC_000913/4601521‑4601707

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑