Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,603,784	(G)_8→9	noncoding (72/87 nt)	leuP ←	tRNA‑Leu

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,603,776	1	.	G	81.5%	379.0 / 95.8	157	noncoding (80/87 nt)	leuP	tRNA‑Leu
Reads supporting (aligned to +/- strand): ref base . (14/15); new base G (83/45); total (97/60)
Fisher's exact test for biased strand distribution p-value = 1.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 216 total aligned reads displayed.

AAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCG  >  NC_000913/4603684‑4603863

aaGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑Ga                                                                                          >  1:5490115/1‑93 (MQ=255)

 aGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GA‑g                                                                                        <  2:384998/93‑1 (MQ=255)

  ggACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATTGCCTCGTTTGGT‑GC‑GA‑gg                                                                                       >  2:4483401/1‑93 (MQ=255)

  ggACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GA‑gg                                                                                       <  1:2672004/93‑1 (MQ=255)

  ggACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GA‑gg                                                                                       >  2:3589733/1‑93 (MQ=255)

   gACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GA‑ggg                                                                                      <  2:1364359/93‑1 (MQ=255)

    acacTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GA‑gggg                                                                                     <  1:831695/93‑1 (MQ=255)

     cacTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GA‑ggggg                                                                                    <  1:1492630/93‑1 (MQ=255)

     cacTAACACCTGAAGCTAGCGCGTCAACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GA‑ggggg                                                                                    <  2:2975262/93‑1 (MQ=255)

       cTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GA‑ggggggg                                                                                  >  1:3957658/1‑93 (MQ=255)

       cTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GA‑ggggggg                                                                                  <  1:2340761/93‑1 (MQ=255)

        tAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GA‑gggggggg                                                                                 <  2:2705531/93‑1 (MQ=255)

        tAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GA‑gggggggg                                                                                 <  1:3104901/93‑1 (MQ=255)

          acacCGGAAGCTGGCGCTTCTCCCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GA‑gggggggggg                                                                               <  1:5312769/93‑3 (MQ=37)

            acCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GA‑ggggggggggct                                                                             >  2:4133113/1‑89 (MQ=255)

            acCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GA‑ggggggggggat                                                                             >  2:4437447/1‑89 (MQ=255)

                 aaGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAGGGGGGGGGGCTTGAAc                                                                         >  1:4154859/1‑93 (MQ=255)

                  aGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAGGGGGGGGGGCTTGAAcc                                                                        >  1:225464/1‑93 (MQ=255)

                  aGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATAGCCTCGTTTGGT‑GC‑GAGGGGGGGGGGCTTGAAcc                                                                        >  1:1741595/1‑93 (MQ=37)

                   gCTAGCGCGTCTACCAATTCCGCCCCCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GA‑GGGGGGGGGCTTGAAcccc                                                                      <  1:1662271/93‑1 (MQ=255)

                   gCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAGGGGGGGGGGCTTGAAccc                                                                       <  2:1490658/93‑1 (MQ=38)

                    cTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAGGGGGGGGGGCTTGAAcccc                                                                      <  1:4618449/93‑1 (MQ=38)

                    cTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAGGGGGGGGGGCTTGAAcccc                                                                      >  1:4103334/1‑93 (MQ=255)

                    cTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑CC‑GAGGGGGGGGGGCTTGAAcccc                                                                      <  1:2711954/93‑1 (MQ=37)

                     tAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAGGGGGGGGGGCTTGAAccccc                                                                     >  2:2126605/1‑93 (MQ=255)

                     tAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAGGGGGGGGGGCTTGAAccccc                                                                     >  1:1468174/1‑93 (MQ=255)

                     tAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GA‑GGGGGGGGGCTTGAACCCCCa                                                                    <  2:6117242/93‑1 (MQ=255)

                      aGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGG‑GC‑GAGGGGGGGGGGCTTGAACCCCCa                                                                    <  2:1531837/93‑1 (MQ=38)

                       gcgcGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAGGGGGGGGGGCTTGAACCCCCAc                                                                   <  1:2299807/93‑1 (MQ=38)

                        cgcgTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAGGGGGGGGGGCTTGAACCCCCACg                                                                  >  1:3809420/1‑93 (MQ=255)

                        cgcgTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTGGATATCGCCTCGTTTGGT‑GC‑GAGGGGGGGGGGCTTGAACCCCCACg                                                                  <  2:5012346/93‑1 (MQ=38)

                         gcgTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAGGGGGGGGGGCTTGAACCCCCACgg                                                                 >  2:5375467/1‑92 (MQ=38)

                         gcgTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAGGGGGGGGGGCTTGAACCCCCACGt                                                                 >  1:3551001/1‑93 (MQ=255)

                         ccgTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GA‑GGGGGGGGGCTTGAACCCCCACGTc                                                                <  2:4676407/92‑1 (MQ=255)

                           gTCTACCAATCCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCCGGTTTGGT‑GC‑GAGGGGGGGGGGCTTGAACCCCCACGTcc                                                               <  2:4797330/93‑1 (MQ=37)

                            tCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAGGGGGGGGGGCTTGAACCCCCACGTCCg                                                              >  1:5887988/1‑93 (MQ=255)

                               aCCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAGGGGGGGGGGGTTGAACCCCCACGGCCGGaa                                                           >  2:3177/1‑93 (MQ=255)

                               aCCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAGGGGGGGGGGCTTGAACCCCCACGTCCGTaa                                                           >  2:3071124/1‑93 (MQ=255)

                                ccAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATGTCGCCTCGTTTGGT‑GC‑GAGGGGGGGGGGCTTGAACCCCCACGTCCGTAAg                                                          >  1:4434944/1‑93 (MQ=37)

                                 cAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAGGGGGGGGGGCTTGAACCCCCCCGTCCGTAAGa                                                         >  1:4292414/1‑93 (MQ=38)

                                 cAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GA‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGaa                                                        >  2:4303902/1‑93 (MQ=255)

                                  aaTTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAGGGGGGGGGGCTTGAACCCCCACGTCCGTAAGaa                                                        >  1:6201081/1‑93 (MQ=255)

                                       cGCCCCCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTGGTTTGGT‑GC‑GAGGGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTa                                                   <  1:5742140/93‑1 (MQ=25)

                                        gCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GA‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAac                                                 >  1:2903099/1‑93 (MQ=255)

                                         ccACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAGGGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAac                                                 >  2:771054/1‑93 (MQ=255)

                                         ccACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAGGGGGGGGGGCTTGAAACCCCACGTCCGTAAGCACACTAac                                                 >  1:437991/1‑93 (MQ=37)

                                          cACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAGGGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACCCTaaac                                                >  2:3983699/1‑91 (MQ=37)

                                          cACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAGGGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAaca                                                <  1:4118224/93‑1 (MQ=37)

                                          cACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GA‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAacac                                               >  2:618477/1‑93 (MQ=255)

                                            ccTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAGGGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACAcc                                              >  2:2309491/1‑93 (MQ=255)

                                             cTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAGGGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCt                                             >  1:2817085/1‑93 (MQ=255)

                                              ttCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAGGGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACCCTAACACCTg                                            >  2:4151121/1‑93 (MQ=25)

                                               tCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAGGGGGGGGGGCTTGGACCCCCACGTCCGTAAGAACAGTAACACCTGa                                           >  1:5803056/1‑93 (MQ=25)

                                               tCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAGGGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGa                                           <  1:4031510/93‑1 (MQ=255)

                                                cGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GA‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAg                                         >  1:736682/1‑93 (MQ=255)

                                                  cacaGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAGGGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGc                                        >  2:1919608/1‑93 (MQ=255)

                                                   acaGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAGGGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCt                                       >  1:6280307/1‑93 (MQ=255)

                                                   acaGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GA‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTa                                      <  2:5159062/93‑1 (MQ=255)

                                                   acaGTCATCTTACTTTTTTTGATATCCCCTCGTTTGGT‑GC‑GAGGGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCt                                       <  1:6214918/93‑1 (MQ=21)

                                                    caGTCATTTTATTTTTTTTAATATCGCCTGTTTTGGG‑CCGGG‑GGGGGGGGGCTTGACCCCCCACGTCCGTAAGAACACTAACACCTGAAGCTa                                      <  2:74259/93‑1 (MQ=12)

                                                    caGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAGGGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTa                                      >  1:3203491/1‑93 (MQ=255)

                                                     aGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAGGGGGGGGGGCTTGAACCCCGAAGCCCGTTACAACACTGACACCTGAAGCTAg                                     >  2:3656576/1‑93 (MQ=21)

                                                      tgtaTCTTACTTTTTTTAATATCGCCTGGTTGGGT‑GC‑GAGGGGGGGGGGCTTGACCCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAgc                                    <  1:3304109/90‑1 (MQ=18)

                                                      gTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GA‑GGGGGGGGGGCTGAAACCCCCCGTCCCTAAGAAAAACAAAACCTGAAACTAacg                                   >  2:2881778/1‑93 (MQ=19)

                                                         aTCTAACTTTTTTTGAAATCACCTCGTTTGGG‑GCGGG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGcgcg                                 <  2:551122/93‑1 (MQ=17)

                                                          tCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAGGGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGt                                >  1:3719410/1‑93 (MQ=255)

                                                          tCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAGGGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGt                                >  2:5473331/1‑93 (MQ=255)

                                                          tCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAGGGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGt                                >  1:4740751/1‑93 (MQ=255)

                                                           cTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAGGGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTc                               >  2:1221919/1‑93 (MQ=255)

                                                            ttACTTTTTTTGATATCGCCTGGTTTGGT‑GC‑GA‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTa                             <  2:3584949/93‑1 (MQ=255)

                                                            ttACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAGGGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCt                              >  2:5951047/1‑93 (MQ=255)

                                                             tACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAGGGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTa                             <  1:1357725/93‑1 (MQ=255)

                                                               cTTTTTTTTGTATCGCCTCGTTTGGA‑GC‑GAGGGGGGGGGGCTTGACCCCCCACGTCCGTAAGAACACTACCCCCTGAAGCTAGCGCGTCTAcc                           <  2:5670606/93‑1 (MQ=17)

                                                                 ttttttTATAGCGCCTTGTTTGGT‑GC‑GAGGGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCaa                         <  2:1720055/93‑1 (MQ=14)

                                                                 ttttttGATATCGCCTCGTTTGGT‑GC‑GAGGGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCaa                         <  1:216990/93‑1 (MQ=255)

                                                                  tttttGATATCGCCTCGTTTGGT‑GC‑GAGGGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAAt                        <  1:3774028/93‑1 (MQ=255)

                                                                     ttGATAGCTCCTCGTGTGGTGGG‑GC‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTcc                     <  1:2579740/93‑1 (MQ=14)

                                                                      tGATATCGCCTCGTTTGGT‑GC‑GAGGGGGGGGGGCTTGAACCCCCCCGGCCGTAAGAACACTAACACCTGAACCAAGCGCGTTTACCCATTCCg                    >  2:5879207/1‑93 (MQ=17)

                                                                      tGATATCGCCTCGTTTGGT‑GC‑GAGGGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTccc                    >  1:3075200/1‑92 (MQ=17)

                                                                      tGATATCGCCTCGTTTGGT‑GC‑GAGGGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCg                    >  2:1208031/1‑93 (MQ=255)

                                                                       gATATCGCCTCGTTTGGT‑GC‑GAGGGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc                   >  1:512491/1‑93 (MQ=255)

                                                                        atatCGCCTCGTTTGGT‑GC‑GAGGGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACAGCTGAAGCTAGCGCGTCTACCAATTCCGcc                  >  1:3257421/1‑93 (MQ=17)

                                                                         aagcgccgcGTTTGGT‑GCGGG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCa                 <  2:620456/90‑1 (MQ=14)

                                                                          tgccccTCTTTGGGT‑GG‑GAGGGGGGGGGGCTTGACCCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCAc                <  2:3459229/89‑1 (MQ=11)

                                                                           tcaccTCGATTGGG‑GCGGG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCAcc               <  2:572517/90‑1 (MQ=11)

                                                                            cGCCTCGTTTGGT‑GC‑GAGGGGGGGGGGGTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCt              >  1:1246624/1‑93 (MQ=14)

                                                                             tccTCGTTTGGT‑GC‑GAGGGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCtt             >  1:98492/2‑93 (MQ=14)

                                                                             gCCTCGTTTGGT‑GC‑GAGGGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCtt             >  1:3161271/1‑93 (MQ=255)

                                                                             gCCTCGTTTGGT‑GC‑GA‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTc            >  2:3698497/1‑93 (MQ=255)

                                                                              ccTCGTTTGGT‑GC‑GA‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCg           <  2:5139746/93‑1 (MQ=255)

                                                                              ccTCGTTTGGC‑GC‑GAGGGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCCATTCCGCCACCTTc            >  1:1074392/1‑93 (MQ=14)

                                                                               cTCGTTTGGT‑GC‑GAGGGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCg           >  1:5163771/1‑93 (MQ=255)

                                                                                   tttGGT‑GC‑GAGGGGGGGGGGCTTGACCCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCa       <  2:4907933/93‑1 (MQ=11)

                                                                                   tttGGT‑GC‑GAGGGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCa       <  1:1920305/93‑1 (MQ=255)

                                                                                   tttGGT‑GC‑GAGGGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCa       >  1:6115912/1‑93 (MQ=255)

                                                                                    ttGGT‑GCGGG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAg      <  2:1840166/93‑1 (MQ=11)

                                                                                    ttGGT‑GC‑GAGGGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAg      <  2:3906980/93‑1 (MQ=255)

                                                                                    ttGGG‑GC‑GAGGGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAg      <  2:2954527/93‑1 (MQ=11)

                                                                                      ggT‑GC‑GA‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGc   >  2:4364935/1‑93 (MQ=255)

                                                                                       gT‑GC‑GG‑GTGGGGGGGCTTGACCCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCg  <  1:483907/93‑1 (MQ=14)

AAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCG  >  NC_000913/4603684‑4603863

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGT  >  NC_000913/4603691‑4603877

CTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑GGGTGGGG                                                                                               >  SRR4302533.3154238/1‑94 (MQ=60)

CTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑GGGGGGGG                                                                                               <  SRR4302533.2355253/94‑1 (MQ=60)

 TAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑GGGGGGGGG                                                                                              <  SRR4302533.3123987/94‑1 (MQ=60)

 TAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑GGGGGGGGG                                                                                              >  SRR4302533.3804432/1‑94 (MQ=60)

  AACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑GGGGGGGGgg                                                                                             >  SRR4302533.797708/1‑92 (MQ=60)

   ACACCGGAAGCTGGCGCTTCTCCCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑GGGGGGGGggc                                                                                            <  SRR4302533.5344608/94‑4 (MQ=60)

     ACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑GGGGGGGGGCTTG                                                                                          >  SRR4302533.3179031/1‑94 (MQ=60)

      CCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑GGGGGGGGGCTTGA                                                                                         >  SRR4302533.1565587/1‑94 (MQ=60)

        TGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑GGGGGGGGggcttgaa                                                                                       >  SRR4302533.3297452/1‑86 (MQ=60)

        TGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑GGGGGGGGggcttgaa                                                                                       >  SRR4302533.4535006/1‑86 (MQ=60)

         GAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAAC                                                                                       >  SRR4302533.4180036/1‑94 (MQ=60)

         GAAGCGAGCGCGTCTACCAATCCCGCCCCCTTCGCACATTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAAC                                                                                       <  SRR4302533.3496514/94‑1 (MQ=60)

          AAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACC                                                                                      >  SRR4302533.226871/1‑94 (MQ=60)

          AAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑GGGGGGGGGCTTGAACCC                                                                                     <  SRR4302533.3121078/94‑1 (MQ=60)

          AAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATAGCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACC                                                                                      >  SRR4302533.1752157/1‑94 (MQ=60)

          AAGCTAGCGCGTCTACCAATTCAGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACC                                                                                      <  SRR4302533.5891245/94‑1 (MQ=60)

            GCTAGCGCGTCTACCAATTCCGCCCCCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑GGGGGGGGGCTTGAACCCCC                                                                                   <  SRR4302533.1672320/94‑1 (MQ=60)

            GCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCC                                                                                    <  SRR4302533.2605961/94‑1 (MQ=60)

            GCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCC                                                                                    >  SRR4302533.4128296/1‑94 (MQ=60)

            GCTAGCGCGTCTACCAAATCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCC                                                                                    >  SRR4302533.1851731/1‑94 (MQ=60)

             CTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCC                                                                                   >  SRR4302533.1477028/1‑94 (MQ=60)

             CTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCC                                                                                   <  SRR4302533.4646437/94‑1 (MQ=60)

             CTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTCC‑GAG‑GGGGGGGGGCTTGAACCCCC                                                                                   <  SRR4302533.2728603/94‑1 (MQ=60)

                GCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCCACG                                                                                <  SRR4302533.2314062/94‑1 (MQ=60)

                GCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCCACG                                                                                >  SRR4302533.3832724/1‑94 (MQ=60)

                 CGCGTTTACCAATTCCGCCCCCTTCGCCCAGTCATCTT‑CCTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCCACGTC                                                                              <  SRR4302533.3236131/94‑1 (MQ=60)

                 CGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCCACGT                                                                               >  SRR4302533.3572876/1‑94 (MQ=60)

                   CGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCCACGTCC                                                                             >  SRR4302533.2716707/1‑94 (MQ=60)

                   CGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCCACGTCC                                                                             >  SRR4302533.5921271/1‑94 (MQ=60)

                    GTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCCACGTCCG                                                                            >  SRR4302533.5923560/1‑94 (MQ=60)

                     TCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑GGGGGGGG‑CTTGAACCCCCACGTCCGTAA                                                                         >  SRR4302533.4150951/1‑94 (MQ=60)

                        ACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATGTCGCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAG                                                                        >  SRR4302533.4461753/1‑94 (MQ=60)

                         CCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGGGGGGGGGGGCTTGAACCCCCACGGCCCTAAG                                                                        >  SRR4302533.2319140/1‑94 (MQ=60)

                         CCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCCCCGTCCGTAAGA                                                                       >  SRR4302533.4318229/1‑94 (MQ=60)

                          CAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAA                                                                      <  SRR4302533.426033/94‑1 (MQ=60)

                          CAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAA                                                                      >  SRR4302533.6238274/1‑94 (MQ=60)

                           AATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAAC                                                                     >  SRR4302533.2486604/1‑94 (MQ=60)

                             TTCCGCCCCCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACAC                                                                   <  SRR4302533.1492111/94‑1 (MQ=60)

                               CCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAA                                                                >  SRR4302533.4900549/1‑94 (MQ=60)

                                CGCCCCCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTGGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAA                                                                <  SRR4302533.5776790/94‑1 (MQ=60)

                                CGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAAC                                                               >  SRR4302533.2920929/1‑94 (MQ=60)

                                 ccctcctcCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAAC                                                               <  SRR4302533.4075527/86‑1 (MQ=60)

                                 GCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAAC                                                               >  SRR4302533.4007973/1‑94 (MQ=60)

                                 GCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAAACCCCACGTCCGTAAGCACACTAAC                                                               >  SRR4302533.440599/1‑94 (MQ=60)

                                  CCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAAGACTAACC                                                              >  SRR4302533.1262868/1‑94 (MQ=60)

                                  CCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACA                                                              >  SRR4302533.5179003/1‑94 (MQ=60)

                                   CACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACAC                                                             <  SRR4302533.4143238/94‑1 (MQ=60)

                                     CCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCT                                                           >  SRR4302533.2834426/1‑94 (MQ=60)

                                      cctccctgtgcgtttttttttttttttggtatccgctttgtttggtgcgggGGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACC                                                            <  SRR4302533.5561452/43‑1 (MQ=21)

                                      CTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTG                                                          >  SRR4302533.6293197/1‑94 (MQ=60)

                                       TTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGGACCCCCACGTCCGTAAGAACAGTAACACCTGA                                                         >  SRR4302533.5838107/1‑94 (MQ=60)

                                        TCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAA                                                        <  SRR4302533.4056050/94‑1 (MQ=60)

                                        TCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAG                                                       >  SRR4302533.741015/1‑94 (MQ=60)

                                         CGCACAGTCTTCTTTTTTTTTTTGATATCGCTTCTTTGGGGGC‑GAG‑GGGGGGGGGCTTGACCCCCCACGTCCGTAAGAACACTAACACCTGAAG                                                       <  SRR4302533.1210307/94‑1 (MQ=36)

                                           caaactgtgatgttattttttttaatagcgccgtgttgggtgcgagGGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAG                                                       <  SRR4302533.57941/48‑1 (MQ=22)

                                           CACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCT                                                     >  SRR4302533.6317795/1‑94 (MQ=60)

                                            ACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTA                                                    >  SRR4302533.3223207/1‑94 (MQ=60)

                                            ACAGTCATCTTACTTTTTTTGATATCCCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTA                                                    <  SRR4302533.6252184/94‑1 (MQ=60)

                                             CAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAATCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAG                                                   >  SRR4302533.2869098/1‑94 (MQ=60)

                                              AGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGGGGGGGGGGGCTTGAACCCCCACGCCCGTAAAAACACTAAAACCTGAAGCTAC                                                   >  SRR4302533.1751349/1‑94 (MQ=60)

                                              AGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGC                                                  <  SRR4302533.821827/94‑1 (MQ=60)

                                               tgtATCTTACTTTTTTTAATATCGCCTGGTTGGGTGC‑GAG‑GGGGGGGGGCTTGACCCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCG                                                 <  SRR4302533.3324513/91‑1 (MQ=37)

                                               GTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCG                                                 <  SRR4302533.1933312/94‑1 (MQ=60)

                                               GTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAAACCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCG                                                 >  SRR4302533.2217754/1‑94 (MQ=60)

                                               GTCATCTTACTTTTTTCGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGACCCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCG                                                 <  SRR4302533.275300/94‑1 (MQ=60)

                                                  ATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGT                                              >  SRR4302533.3742242/1‑94 (MQ=60)

                                                  ATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGT                                              >  SRR4302533.4769495/1‑94 (MQ=60)

                                                  ATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGT                                              >  SRR4302533.6214409/1‑94 (MQ=60)

                                                   tttttttttttttttattcccccttttttgggggggggGGGGGGGGGCTTGACCCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCG                                               <  SRR4302533.20491/56‑1 (MQ=8)

                                                    CTTACTTTTTTTTATATCGCCTCGTTTGGTGC‑GA‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTA                                           <  SRR4302533.139884/94‑1 (MQ=60)

                                                    CTTACTTTTTTTGATATCGCCTCGTTTGGTGCGGG‑‑GGGGGGGGGCTTGACCCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCT                                            <  SRR4302533.2625340/94‑1 (MQ=58)

                                                    CTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCT                                            >  SRR4302533.2298777/1‑94 (MQ=60)

                                                    CTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTA                                           >  SRR4302533.6251031/1‑94 (MQ=60)

                                                     TTACTTTTTTATATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGGTCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTA                                           <  SRR4302533.4223535/94‑1 (MQ=43)

                                                      TACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTAC                                          <  SRR4302533.1365887/94‑1 (MQ=60)

                                                        CTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCA                                        >  SRR4302533.3950904/1‑94 (MQ=59)

                                                          TTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAAT                                      <  SRR4302533.218350/94‑1 (MQ=59)

                                                          TTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAAT                                      >  SRR4302533.475986/1‑94 (MQ=54)

                                                           tttttttaaattcgcctgttttggggggggGGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAA                                       <  SRR4302533.6323083/64‑1 (MQ=9)

                                                           TTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATT                                     <  SRR4302533.3797162/94‑1 (MQ=56)

                                                            TTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTC                                    >  SRR4302533.2943553/1‑94 (MQ=52)

                                                              TTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCC                                  >  SRR4302533.3094073/1‑94 (MQ=47)

                                                               TGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGC                                 <  SRR4302533.722596/94‑1 (MQ=42)

                                                               TGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGC                                 >  SRR4302533.5839285/1‑94 (MQ=45)

                                                               TGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGC                                 >  SRR4302533.515536/1‑94 (MQ=45)

                                                                ttgatagctcctcgtgtggtggggcGGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCG                                  <  SRR4302533.2595615/69‑1 (MQ=8)

                                                                GATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACAGCTGAAGCTAGCGCGTCTACCAATTCCGCC                                >  SRR4302533.3277510/1‑94 (MQ=38)

                                                                GATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCC                                >  SRR4302533.5257842/1‑94 (MQ=42)

                                                                 ATATCGCCTCGTTTGGTGC‑GAGGGGGGGGGGGGTTGAACCCCCCCGCCCGAAAGAACACTAAACCCTGAAGCTAGCGCGTCTACCAATTtcccc                                >  SRR4302533.3592524/1‑89 (MQ=28)

                                                                 ATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCA                               >  SRR4302533.3279795/1‑94 (MQ=40)

                                                                  TATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCAC                              >  SRR4302533.3682206/1‑94 (MQ=38)

                                                                    TCGCCTCGTTTGGTGC‑GAG‑GGGGGGGGGGTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCT                            >  SRR4302533.1254066/1‑94 (MQ=31)

                                                                    TCGCCTCGTTTGGTGC‑GA‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTT                           >  SRR4302533.3760775/1‑94 (MQ=35)

                                                                     CTCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTT                           >  SRR4302533.99102/1‑94 (MQ=25)

                                                                     CGCCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTT                           >  SRR4302533.3180724/1‑94 (MQ=30)

                                                                     CGCCTCGTTTGGGGC‑GAG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTT                           <  SRR4302533.6130370/94‑1 (MQ=22)

                                                                      GCCTCGTTTGGTGC‑GA‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCG                         >  SRR4302533.4798217/1‑94 (MQ=30)

                                                                      GCCTCGTTTGGCGC‑GAG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCCATTCCGCCACCTTC                          >  SRR4302533.1080725/1‑94 (MQ=19)

                                                                       GCTCGTTTGGGGC‑GAG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCG                         <  SRR4302533.1889487/94‑1 (MQ=10)

                                                                       CCTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCG                         >  SRR4302533.5194950/1‑94 (MQ=23)

                                                                        CTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCCCCGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCCCCACCTTCGC                        >  SRR4302533.578388/1‑94 (MQ=19)

                                                                        CTCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGC                        >  SRR4302533.4618036/1‑94 (MQ=21)

                                                                         gctgtttgtgtgggagGGGGGGGGGCTTGACCCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCG                         <  SRR4302533.228447/78‑1 (MQ=7)

                                                                         TCGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCC                       >  SRR4302533.965259/1‑94 (MQ=21)

                                                                          CGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCC                      >  SRR4302533.3768326/1‑94 (MQ=21)

                                                                          CGTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCCACGACCGTAAAGACACTAACACCAGAAGCTAGGGCGTCTACCAATTGCGCCACCTTCGCCC                      >  SRR4302533.5306146/1‑94 (MQ=57)

                                                                           cttgGGTGC‑GA‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAG                    <  SRR4302533.5442871/90‑1 (MQ=23)

                                                                           GTTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCA                     >  SRR4302533.6152809/1‑94 (MQ=21)

                                                                            TTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAG                    >  SRR4302533.5197325/1‑94 (MQ=21)

                                                                            TTTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAG                    <  SRR4302533.1932027/94‑1 (MQ=21)

                                                                              TGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTG                  <  SRR4302533.6127574/94‑1 (MQ=26)

                                                                              TGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCCCCCCGTG                  >  SRR4302533.4869867/1‑94 (MQ=5)

                                                                               ttggggggagGGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGT                   <  SRR4302533.1268247/84‑1 (MQ=26)

                                                                                GTGC‑GG‑‑GTGGGGGGGCTTGACCCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGA               <  SRR4302533.486799/94‑1 (MQ=31)

                                                                                 TGC‑GAG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTTGCCCAGTGCGA               >  SRR4302533.2122093/1‑94 (MQ=27)

                                                                                  gggcgagGGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCG                <  SRR4302533.3640568/87‑1 (MQ=34)

                                                                                      G‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAAT          <  SRR4302533.3586366/94‑1 (MQ=47)

                                                                                      G‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCACTGCGAGCAAT          >  SRR4302533.2812324/1‑94 (MQ=33)

                                                                                          GGGGGGGGCTTGACCCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATAT        <  SRR4302533.4726853/94‑1 (MQ=51)

                                                                                          GGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATAT        <  SRR4302533.873281/94‑1 (MQ=53)

                                                                                          GGGGGGGGCTTCAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATAT        <  SRR4302533.342901/94‑1 (MQ=51)

                                                                                            GGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCA      <  SRR4302533.2668180/94‑1 (MQ=58)

                                                                                             GGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAA     >  SRR4302533.5709151/1‑94 (MQ=60)

                                                                                              GGGGCTTGACCCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAAC    <  SRR4302533.2416894/94‑1 (MQ=60)

                                                                                              GGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAAC    >  SRR4302533.38712/1‑94 (MQ=60)

                                                                                               GGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACG   >  SRR4302533.5253444/1‑94 (MQ=60)

                                                                                               GGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACG   <  SRR4302533.2599919/94‑1 (MQ=60)

                                                                                               GGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACG   >  SRR4302533.3525723/1‑94 (MQ=60)

                                                                                                GGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGT  >  SRR4302533.2621594/1‑94 (MQ=60)

                                                                                                GGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGT  >  SRR4302533.5713334/1‑94 (MQ=60)

CTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGT  >  NC_000913/4603691‑4603877

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`