Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,621,644	A→G	W15R (TGG→CGG)	lplA ←	lipoate‑protein ligase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,621,644	0	A	G	100.0%	780.8 / NA	240	W15R (TGG→CGG)	lplA	lipoate‑protein ligase A
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (108/132); total (108/132)

Only 100 of 249 total aligned reads displayed.

CTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTT  >  NC_000913/4621554‑4621733

ctacCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACcgcg                                                                                         <  2:5597030/93‑1 (MQ=255)

ctacCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACcgcg                                                                                         >  2:5265537/1‑93 (MQ=255)

ctacCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACcgcg                                                                                         >  1:799479/1‑93 (MQ=255)

 tacCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCgg                                                                                        <  2:1384417/93‑1 (MQ=255)

  acCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCggg                                                                                       <  1:1120075/93‑1 (MQ=255)

  acCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCggg                                                                                       <  1:2578542/93‑1 (MQ=255)

     gtgtCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCg                                                                                    >  2:4596265/1‑93 (MQ=255)

       gtCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTa                                                                                  <  1:3846334/93‑1 (MQ=255)

       gtCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTa                                                                                  <  2:4458478/93‑1 (MQ=255)

        tCGGCTTTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTaa                                                                                 <  1:2002968/93‑1 (MQ=255)

         cGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAag                                                                                <  2:5060354/93‑1 (MQ=255)

            cATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGt                                                                             <  2:2449668/93‑1 (MQ=255)

            cATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGt                                                                             >  1:5704813/1‑93 (MQ=255)

             aTTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTACGAGTc                                                                            >  1:3973279/1‑93 (MQ=255)

             aTTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTc                                                                            <  1:4664624/93‑1 (MQ=255)

              ttGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTCAGAGTCa                                                                           >  1:1478140/1‑93 (MQ=255)

              ttGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCa                                                                           <  1:3539830/93‑1 (MQ=255)

              ttGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCa                                                                           >  1:5158915/1‑93 (MQ=255)

              ttGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCa                                                                           <  1:1534141/93‑1 (MQ=255)

                gcgcCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCaga                                                                         <  1:1694738/93‑1 (MQ=255)

                 cgcCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCagag                                                                        >  1:3000069/1‑93 (MQ=255)

                  gcCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAgaga                                                                       <  2:3292417/93‑1 (MQ=255)

                   ccAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGAt                                                                      >  1:3932627/1‑93 (MQ=255)

                    cAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATg                                                                     <  2:5839184/93‑1 (MQ=255)

                     agagAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGa                                                                    >  2:260927/1‑93 (MQ=255)

                     agagAAACAGAACGCGCTGCGTGGCGGCCATTTGGCGAAAAATACGCTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGa                                                                    <  2:3641073/93‑1 (MQ=255)

                        gaAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCa                                                                 <  1:6134405/93‑1 (MQ=255)

                         aaaCAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAg                                                                <  2:3721009/93‑1 (MQ=255)

                          aaCAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAgg                                                               <  2:2050503/93‑1 (MQ=255)

                          aaCAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAgg                                                               <  1:3506636/93‑1 (MQ=255)

                           aCAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGc                                                              <  2:1009071/93‑1 (MQ=255)

                           aCAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGc                                                              <  2:4981933/93‑1 (MQ=255)

                           aCAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGc                                                              <  2:884037/93‑1 (MQ=255)

                           aCAGAACGCGCTGCGTGGCGGGCATTTGGCCAAAAATACACTCTTCCCCCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGc                                                              <  1:3137278/93‑1 (MQ=255)

                            cAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCg                                                             <  1:2437533/93‑1 (MQ=255)

                             aGAACGCGCTGCGTGGCGGTCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGt                                                            <  1:2208809/93‑1 (MQ=255)

                              gAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTa                                                           >  2:3672123/1‑93 (MQ=255)

                               aaCGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTaa                                                          >  2:691191/1‑93 (MQ=255)

                                aCGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAAt                                                         <  1:1841385/93‑1 (MQ=255)

                                 cgcgCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAAtg                                                        >  1:5534900/1‑93 (MQ=255)

                                   cgcTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAAtgtg                                                      <  1:4585894/93‑1 (MQ=255)

                                       gCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACa                                                  <  2:42348/93‑1 (MQ=255)

                                       gCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACa                                                  >  1:6007471/1‑93 (MQ=255)

                                        cGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACAt                                                 >  1:4739126/1‑93 (MQ=255)

                                         gTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATa                                                <  1:2331271/93‑1 (MQ=255)

                                           ggCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAAc                                              <  2:4036497/93‑1 (MQ=255)

                                            gCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACg                                             <  1:3601068/93‑1 (MQ=255)

                                              gggCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGAt                                           <  2:3564225/93‑1 (MQ=255)

                                              gggCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGAt                                           >  2:5122453/1‑93 (MQ=255)

                                              gggCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGAt                                           >  1:2406319/1‑93 (MQ=255)

                                              gggCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGAt                                           <  2:2991818/93‑1 (MQ=255)

                                               ggCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGAtt                                          >  2:3900297/1‑93 (MQ=255)

                                               ggCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGAtt                                          >  2:2115232/1‑93 (MQ=255)

                                               ggCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGAtt                                          >  2:3494002/1‑93 (MQ=255)

                                               ggCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGAtt                                          <  2:5757365/93‑1 (MQ=255)

                                                gCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGAttt                                         >  1:1368850/1‑93 (MQ=255)

                                                  aTTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTcc                                       >  2:5978816/1‑93 (MQ=255)

                                                    ttGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGCGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCtt                                     <  1:4431473/93‑1 (MQ=255)

                                                    ttGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCtt                                     <  1:2640938/93‑1 (MQ=255)

                                                      ggCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTc                                   >  1:3052042/1‑93 (MQ=255)

                                                      ggCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTc                                   <  2:1500005/93‑1 (MQ=255)

                                                      ggCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTc                                   >  2:251460/1‑93 (MQ=255)

                                                         gAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTg                                <  1:5822886/93‑1 (MQ=255)

                                                           aaaaTACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTgta                              <  1:4462934/93‑1 (MQ=255)

                                                             aaTACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTgtagt                            >  2:2361402/1‑93 (MQ=255)

                                                              aTACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTgtagta                           <  1:5404388/93‑1 (MQ=255)

                                                              aTACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTgtagta                           <  1:6278707/93‑1 (MQ=255)

                                                               tACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTaa                          <  1:2789278/93‑1 (MQ=255)

                                                               tACACTCTTCCACCGCCAGGTTAAACCGCGGGGCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTaa                          >  2:1696428/1‑93 (MQ=255)

                                                                acacTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAAt                         >  2:1888058/1‑93 (MQ=255)

                                                                acacTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAAt                         >  2:3246812/1‑93 (MQ=255)

                                                                acacTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAAt                         <  1:4772467/93‑1 (MQ=255)

                                                                acacTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAAt                         <  1:5460726/93‑1 (MQ=255)

                                                                 cacTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATg                        <  2:1153919/93‑1 (MQ=255)

                                                                 cacTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATg                        <  1:2862778/93‑1 (MQ=255)

                                                                 cacTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATg                        <  1:5037982/93‑1 (MQ=255)

                                                                  acTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATgg                       <  2:4442250/93‑1 (MQ=255)

                                                                   ctctTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATggg                      <  2:4720982/93‑1 (MQ=255)

                                                                    tctTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGt                     >  1:2825856/1‑93 (MQ=255)

                                                                         cACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCActc                <  2:2863719/93‑1 (MQ=255)

                                                                          aCCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCActct               >  1:1069491/1‑93 (MQ=255)

                                                                             gCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCtttt            >  1:1492813/1‑93 (MQ=255)

                                                                             gCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCtttt            <  2:5564385/93‑1 (MQ=255)

                                                                              ccAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCttttt           >  2:623431/1‑93 (MQ=255)

                                                                              ccAGGTGAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCttttt           <  2:658844/93‑1 (MQ=255)

                                                                               cAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCtttttt          >  1:1268986/1‑93 (MQ=255)

                                                                                aGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTtc         >  1:3085715/1‑93 (MQ=255)

                                                                                 ggTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCtttttttt        >  2:6298718/1‑91 (MQ=255)

                                                                                 ggTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTtct        >  1:5880535/1‑93 (MQ=255)

                                                                                 ggTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTtct        <  1:5851460/93‑1 (MQ=255)

                                                                                  gTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTtctc       <  1:2519792/93‑1 (MQ=255)

                                                                                  gTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTtctc       <  1:1385634/93‑1 (MQ=255)

                                                                                  gTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTtctc       <  1:5179865/93‑1 (MQ=255)

                                                                                   ttAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCg      <  2:5411927/93‑1 (MQ=255)

                                                                                     aaaCCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCt    >  1:411561/1‑93 (MQ=255)

                                                                                     aaaCCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCt    >  1:3153699/1‑93 (MQ=255)

                                                                                      aaCCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCtt   >  1:3294479/1‑93 (MQ=255)

                                                                                      aaCCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCtt   <  2:5137879/93‑1 (MQ=255)

                                                                                      aaCCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCtt   <  1:1978997/93‑1 (MQ=255)

                                                                                       aCCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCttt  >  1:4493699/1‑93 (MQ=255)

CTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTT  >  NC_000913/4621554‑4621733

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTC  >  NC_000913/4621552‑4621737

TACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGC                                                                                              >  SRR4302533.417837/1‑94 (MQ=60)

 ACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCG                                                                                             >  SRR4302533.804182/1‑94 (MQ=60)

 ACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCG                                                                                             <  SRR4302533.1437757/94‑1 (MQ=60)

  CTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGG                                                                                            >  SRR4302533.3655577/1‑94 (MQ=60)

  CTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGG                                                                                            <  SRR4302533.2421592/94‑1 (MQ=60)

  CTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGG                                                                                            >  SRR4302533.5130441/1‑94 (MQ=60)

   TACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGG                                                                                           <  SRR4302533.5871249/94‑1 (MQ=60)

    ACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGT                                                                                          <  SRR4302533.1126719/94‑1 (MQ=60)

    ACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGT                                                                                          <  SRR4302533.2594411/94‑1 (MQ=60)

     CCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTC                                                                                         >  SRR4302533.1491598/1‑94 (MQ=60)

     CCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTC                                                                                         <  SRR4302533.6174413/94‑1 (MQ=60)

      CGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCG                                                                                        >  SRR4302533.5719429/1‑94 (MQ=60)

        TGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTA                                                                                      >  SRR4302533.1173427/1‑94 (MQ=60)

         GTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAA                                                                                     <  SRR4302533.3869822/94‑1 (MQ=60)

         GTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAA                                                                                     >  SRR4302533.3063132/1‑94 (MQ=60)

         GTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAA                                                                                     >  SRR4302533.1524445/1‑94 (MQ=60)

          TCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAG                                                                                    <  SRR4302533.2015304/94‑1 (MQ=60)

          TCGGCATTGCGCCAGAGAAACAGAACGAGCTGCGTGGCGGGCGTTTGGCGAAAAATACACTCTTCCCCCACCAGGATAAACCGCGGGTCGTccg                                                                                    >  SRR4302533.2720802/1‑91 (MQ=60)

          TCGCCTTTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAG                                                                                    <  SRR4302533.2015305/94‑1 (MQ=60)

           CGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGA                                                                                   >  SRR4302533.3242026/1‑94 (MQ=60)

           CGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGA                                                                                   <  SRR4302533.6145425/94‑1 (MQ=60)

            GGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAG                                                                                  <  SRR4302533.4780627/94‑1 (MQ=60)

             GCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGT                                                                                 >  SRR4302533.5739272/1‑94 (MQ=60)

             GCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGT                                                                                 >  SRR4302533.2382779/1‑94 (MQ=60)

              CATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTACGAGTC                                                                                >  SRR4302533.3997472/1‑94 (MQ=60)

              CATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTC                                                                                <  SRR4302533.2721894/94‑1 (MQ=60)

               ATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTCAGAGTCA                                                                               >  SRR4302533.1487069/1‑94 (MQ=60)

               ATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCA                                                                               <  SRR4302533.4692885/94‑1 (MQ=60)

               ATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCA                                                                               >  SRR4302533.5190055/1‑94 (MQ=60)

                TTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAG                                                                              <  SRR4302533.3561642/94‑1 (MQ=60)

                TTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAG                                                                              <  SRR4302533.1543357/94‑1 (MQ=60)

                 TGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGA                                                                             <  SRR4302533.3995579/94‑1 (MQ=60)

                  GCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAG                                                                            <  SRR4302533.4830329/94‑1 (MQ=60)

                  GCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAG                                                                            <  SRR4302533.1705005/94‑1 (MQ=60)

                  GCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAG                                                                            >  SRR4302533.3018416/1‑94 (MQ=60)

                   CGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGA                                                                           >  SRR4302533.3725014/1‑94 (MQ=60)

                   CGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGA                                                                           <  SRR4302533.3082442/94‑1 (MQ=60)

                   CGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGA                                                                           >  SRR4302533.5565469/1‑94 (MQ=60)

                    GCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGAT                                                                          <  SRR4302533.5275254/94‑1 (MQ=60)

                    GCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGAT                                                                          >  SRR4302533.3956633/1‑94 (MQ=60)

                     CCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATG                                                                         <  SRR4302533.5891981/94‑1 (MQ=60)

                     CCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATG                                                                         >  SRR4302533.1783845/1‑94 (MQ=60)

                     CCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATG                                                                         >  SRR4302533.219842/1‑94 (MQ=60)

                        GAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGC                                                                      <  SRR4302533.2678163/94‑1 (MQ=60)

                          GAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAG                                                                    <  SRR4302533.6171369/94‑1 (MQ=60)

                          GAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAG                                                                    <  SRR4302533.3385220/94‑1 (MQ=60)

                           AAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGG                                                                   <  SRR4302533.51239/94‑1 (MQ=60)

                            AACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGC                                                                  <  SRR4302533.2610641/94‑1 (MQ=60)

                            AACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGC                                                                  <  SRR4302533.3528258/94‑1 (MQ=60)

                             ACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCG                                                                 <  SRR4302533.463708/94‑1 (MQ=60)

                             ACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCG                                                                 >  SRR4302533.2815833/1‑94 (MQ=60)

                             ACAGAACGCGCTGCGTGGCGGGCATTTGGCCAAAAATACACTCTTCCCCCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCG                                                                 <  SRR4302533.3156567/94‑1 (MQ=60)

                              CAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGT                                                                <  SRR4302533.2452581/94‑1 (MQ=60)

                               AGAACGCGCTGCGTGGCGGTCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTA                                                               <  SRR4302533.2222432/94‑1 (MQ=60)

                                  ACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATG                                                            >  SRR4302533.5439297/1‑94 (MQ=60)

                                  ACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATG                                                            <  SRR4302533.1852616/94‑1 (MQ=60)

                                  ACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATG                                                            >  SRR4302533.5568315/1‑94 (MQ=60)

                                   CGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGT                                                           <  SRR4302533.826397/94‑1 (MQ=60)

                                    GCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTG                                                          >  SRR4302533.6030170/1‑94 (MQ=60)

                                    GCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTG                                                          >  SRR4302533.3162441/1‑94 (MQ=60)

                                     CGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGG                                                         <  SRR4302533.4613674/94‑1 (MQ=60)

                                     CGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGG                                                         <  SRR4302533.4688315/94‑1 (MQ=60)

                                     CGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGG                                                         >  SRR4302533.55547/1‑94 (MQ=60)

                                      GCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGA                                                        >  SRR4302533.1522590/1‑94 (MQ=60)

                                      GCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGA                                                        >  SRR4302533.1337399/1‑94 (MQ=60)

                                       CTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGAC                                                       >  SRR4302533.966441/1‑94 (MQ=60)

                                       CTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGAC                                                       >  SRR4302533.2878851/1‑94 (MQ=60)

                                       CTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGAC                                                       <  SRR4302533.1391168/94‑1 (MQ=60)

                                        TGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACA                                                      >  SRR4302533.6043865/1‑94 (MQ=60)

                                        TGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACA                                                      <  SRR4302533.5781707/94‑1 (MQ=60)

                                        TGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACA                                                      <  SRR4302533.4228603/94‑1 (MQ=60)

                                         GCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACAT                                                     >  SRR4302533.2274807/1‑94 (MQ=60)

                                         GCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACAT                                                     >  SRR4302533.4767861/1‑94 (MQ=60)

                                           GTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAA                                                   >  SRR4302533.1429173/1‑94 (MQ=60)

                                           GTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAA                                                   <  SRR4302533.3532880/94‑1 (MQ=60)

                                           GTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAA                                                   <  SRR4302533.2345704/94‑1 (MQ=60)

                                            TGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAAC                                                  <  SRR4302533.3534098/94‑1 (MQ=60)

                                             GGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACG                                                 >  SRR4302533.4528581/1‑94 (MQ=60)

                                             GGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACG                                                 >  SRR4302533.1784170/1‑94 (MQ=60)

                                              GCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGA                                                <  SRR4302533.3623226/94‑1 (MQ=60)

                                               CGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGAT                                               <  SRR4302533.3139793/94‑1 (MQ=60)

                                               CGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGAT                                               >  SRR4302533.2421183/1‑94 (MQ=60)

                                                GGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATT                                              >  SRR4302533.4168011/1‑94 (MQ=60)

                                                GGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATT                                              <  SRR4302533.1866347/94‑1 (MQ=60)

                                                GGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATT                                              >  SRR4302533.3118181/1‑94 (MQ=60)

                                                GGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATT                                              >  SRR4302533.358366/1‑94 (MQ=60)

                                                 GGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTT                                             >  SRR4302533.13465/1‑94 (MQ=60)

                                                 GGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTT                                             >  SRR4302533.1377081/1‑94 (MQ=60)

                                                 GGCATTTGGCGAAAAAGACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTT                                             <  SRR4302533.3021477/94‑1 (MQ=60)

                                                  GCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTC                                            >  SRR4302533.4506877/1‑94 (MQ=60)

                                                    ATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCT                                          >  SRR4302533.5921656/1‑94 (MQ=60)

                                                      TTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGCGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTT                                        <  SRR4302533.4458263/94‑1 (MQ=60)

                                                      TTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTT                                        <  SRR4302533.2657168/94‑1 (MQ=60)

                                                       TGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTC                                       >  SRR4302533.3070733/1‑94 (MQ=60)

                                                         GCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTT                                     <  SRR4302533.2003587/94‑1 (MQ=60)

                                                          CGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTG                                    >  SRR4302533.3986631/1‑94 (MQ=60)

                                                          CGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTG                                    >  SRR4302533.254577/1‑94 (MQ=60)

                                                          CGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTG                                    <  SRR4302533.165288/94‑1 (MQ=60)

                                                           GAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGT                                   <  SRR4302533.5858057/94‑1 (MQ=60)

                                                            AAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTA                                  <  SRR4302533.2847831/94‑1 (MQ=60)

                                                             AAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAG                                 <  SRR4302533.4515167/94‑1 (MQ=60)

                                                             AAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAG                                 <  SRR4302533.4489927/94‑1 (MQ=60)

                                                             AAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAG                                 <  SRR4302533.1082102/94‑1 (MQ=60)

                                                                ATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAA                              <  SRR4302533.5436864/94‑1 (MQ=60)

                                                                ATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAA                              <  SRR4302533.4479102/94‑1 (MQ=60)

                                                                ATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAA                              <  SRR4302533.6316192/94‑1 (MQ=60)

                                                                 TACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAAT                             <  SRR4302533.2806425/94‑1 (MQ=60)

                                                                 TACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAAT                             >  SRR4302533.3248076/1‑94 (MQ=60)

                                                                  ACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATG                            <  SRR4302533.2451836/94‑1 (MQ=60)

                                                                  ACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATG                            <  SRR4302533.4801390/94‑1 (MQ=60)

                                                                  ACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATG                            <  SRR4302533.5493624/94‑1 (MQ=60)

                                                                   CACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGG                           <  SRR4302533.5068489/94‑1 (MQ=60)

                                                                   CACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGG                           <  SRR4302533.2546478/94‑1 (MQ=60)

                                                                   CACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGG                           <  SRR4302533.2880388/94‑1 (MQ=60)

                                                                    ACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGG                          >  SRR4302533.4770564/1‑94 (MQ=60)

                                                                     CTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTTGTAATGGGT                         >  SRR4302533.2901787/1‑94 (MQ=60)

                                                                     CTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGT                         >  SRR4302533.2843244/1‑94 (MQ=60)

                                                                     CTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGT                         >  SRR4302533.4712668/1‑94 (MQ=60)

                                                                     CTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGT                         <  SRR4302533.2521375/94‑1 (MQ=60)

                                                                       CTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCA                       >  SRR4302533.1456844/1‑94 (MQ=60)

                                                                          CCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTC                    >  SRR4302533.5268771/1‑94 (MQ=60)

                                                                           CACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCT                   >  SRR4302533.1075789/1‑94 (MQ=60)

                                                                              CGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTT                >  SRR4302533.1501816/1‑94 (MQ=60)

                                                                              CGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTT                <  SRR4302533.5752838/94‑1 (MQ=60)

                                                                              CGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTT                <  SRR4302533.3891226/94‑1 (MQ=60)

                                                                               GCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTT               <  SRR4302533.630847/94‑1 (MQ=60)

                                                                                CCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTT              >  SRR4302533.1276582/1‑94 (MQ=60)

                                                                                 CAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTC             >  SRR4302533.3104686/1‑94 (MQ=60)

                                                                                  AGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCT            >  SRR4302533.759006/1‑94 (MQ=60)

                                                                                  AGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCT            >  SRR4302533.5916071/1‑94 (MQ=60)

                                                                                   GGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTC           <  SRR4302533.5886837/94‑1 (MQ=60)

                                                                                    GTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCG          <  SRR4302533.2535319/94‑1 (MQ=60)

                                                                                    GTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCG          <  SRR4302533.5211147/94‑1 (MQ=60)

                                                                                    GTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCG          <  SRR4302533.1393972/94‑1 (MQ=60)

                                                                                      TAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCT        <  SRR4302533.2278713/94‑1 (MQ=60)

                                                                                      TAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCT        <  SRR4302533.4027014/94‑1 (MQ=60)

                                                                                      TAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCT        >  SRR4302533.414031/1‑94 (MQ=60)

                                                                                      TAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCT        >  SRR4302533.3173087/1‑94 (MQ=60)

                                                                                       AAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTT       >  SRR4302533.3314812/1‑94 (MQ=60)

                                                                                        AACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTT      <  SRR4302533.1991117/94‑1 (MQ=60)

                                                                                        AACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTT      <  SRR4302533.5196352/94‑1 (MQ=60)

                                                                                        AACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTT      >  SRR4302533.4520912/1‑94 (MQ=60)

                                                                                          CCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCT    <  SRR4302533.3721192/94‑1 (MQ=60)

                                                                                          CCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCT    >  SRR4302533.5676820/1‑94 (MQ=60)

                                                                                          CCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCT    <  SRR4302533.1937626/94‑1 (MQ=60)

                                                                                           CGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTT   <  SRR4302533.2695154/94‑1 (MQ=60)

                                                                                            GCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTC  <  SRR4302533.928726/94‑1 (MQ=60)

TACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTC  >  NC_000913/4621552‑4621737

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`