Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 647 270.1 21258240 98.4% 20918108 94.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,376,055 T→C T78A (ACC→GCC)  rplM ← 50S ribosomal subunit protein L13

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,376,0550TC99.4% 1800.7 / ‑3.0 523T78A (ACC→GCC) rplM50S ribosomal subunit protein L13
Reads supporting (aligned to +/- strand):  ref base T (0/3);  new base C (257/263);  total (257/266)
Fisher's exact test for biased strand distribution p-value = 2.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 557 total aligned reads displayed.

ATGCCTTTAACCGCGATTTCAATCACACGCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGTGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAACGATGATGTAATCACCGGTATCTAC  >  NC_000913/3375964‑3376145
                                                                                           |                                                                                          
aTGCCTTTAACCGCGATTTCAATCACACGCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGgcgt                                                                                          >  2:490237/1‑94 (MQ=255)
aTGCCTTTAACCGCGATTTCAATCACACGCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGgcgt                                                                                          <  2:8428648/94‑1 (MQ=255)
    cTTTAACCGCGATTTCAATCACACGCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCgtggtg                                                                                      >  2:8243548/1‑94 (MQ=255)
     tttAACCGCGATTTCAATCACACGCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGa                                                                                     <  1:5210519/94‑1 (MQ=255)
     tttAACCGCGATTTCAATCACACGCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGa                                                                                     >  2:3581269/1‑94 (MQ=255)
     tttAACCGCGATTTCAATCACACGCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGa                                                                                     <  2:3334302/94‑1 (MQ=255)
      ttAACCGCGATTTCAATCACACGCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGAt                                                                                    <  2:620499/94‑1 (MQ=255)
             cgATTTCAATCACACGCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTacac                                                                             >  1:10580187/1‑94 (MQ=255)
               aTTTCAATCACACGCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACtt                                                                           <  1:8354907/94‑1 (MQ=255)
               aTTTCAATCACACGCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACtt                                                                           <  1:1046702/94‑1 (MQ=255)
               aTTTCAATCACACGCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACtt                                                                           <  2:4789770/94‑1 (MQ=255)
               aTTTCAATCACACGCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACtt                                                                           >  2:4079050/1‑94 (MQ=255)
                  tCAATCACACGCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTgc                                                                        >  1:8890715/1‑93 (MQ=255)
                   cAATCACACGCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTc                                                                       >  2:4397685/1‑94 (MQ=255)
                   cAATCACACGCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTc                                                                       >  2:5676581/1‑94 (MQ=255)
                    aaTCACACGCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCa                                                                      <  1:2237014/94‑1 (MQ=255)
                     aTCACACGCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAg                                                                     >  1:10271954/1‑94 (MQ=255)
                     aTCACACGCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAg                                                                     >  2:5741039/1‑94 (MQ=255)
                        acacGCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTAc                                                                  <  1:8098177/94‑1 (MQ=255)
                        acacGCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTAc                                                                  >  1:944232/1‑94 (MQ=255)
                        acacGCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTAc                                                                  <  2:918141/94‑1 (MQ=255)
                        acacGCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTAc                                                                  >  1:3671666/1‑94 (MQ=255)
                         cacGCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTAcc                                                                 >  1:9037997/1‑93 (MQ=255)
                         cacGCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACg                                                                 >  1:3361493/1‑94 (MQ=255)
                          acGCTCAGGACGGCGAGCAATCATCTCTTCGAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGc                                                                >  1:3026837/1‑94 (MQ=255)
                          acGCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGc                                                                >  1:7240877/1‑94 (MQ=255)
                            gCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCtt                                                              >  2:7518836/1‑94 (MQ=255)
                            gCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCtt                                                              >  1:8443833/1‑94 (MQ=255)
                            gCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCtt                                                              >  1:4001062/1‑94 (MQ=255)
                              tCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCttgt                                                            >  2:8532915/1‑94 (MQ=255)
                              tCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCttgt                                                            >  1:1588169/1‑94 (MQ=255)
                                  gACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGcc                                                        <  1:3849088/94‑1 (MQ=255)
                                    cGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCgg                                                      <  2:3222730/94‑1 (MQ=255)
                                    cGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCgg                                                      <  1:3499444/94‑1 (MQ=255)
                                     ggCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATCCCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGt                                                     <  2:3812184/94‑1 (MQ=255)
                                       cGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTa                                                   >  1:189412/1‑94 (MQ=255)
                                         aGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACa                                                 <  2:1357799/94‑1 (MQ=255)
                                         aGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACa                                                 <  2:2667426/94‑1 (MQ=255)
                                          gCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAg                                                <  2:627495/94‑1 (MQ=255)
                                          gCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAg                                                <  2:1955064/94‑1 (MQ=255)
                                          gCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAg                                                <  1:6653283/94‑1 (MQ=255)
                                          gCAATCATCTCTCCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACTCTTTGTCAGTACACTTGTTCTCGGTTACAg                                                >  2:4252956/1‑94 (MQ=255)
                                           cAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGc                                               >  2:7290205/1‑94 (MQ=255)
                                           cAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGc                                               <  2:2307308/94‑1 (MQ=255)
                                           cAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGc                                               >  1:435656/1‑94 (MQ=255)
                                            aaTCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCa                                              <  1:10003181/94‑1 (MQ=255)
                                            aaTCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCa                                              >  2:6663676/1‑94 (MQ=255)
                                               catcTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACt                                           >  1:820623/1‑94 (MQ=255)
                                               catcTCTTCAAAGGTCGCTTGTTTGATACAACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGGTTGTTGCTGGTTACAGCAACt                                           >  1:4109113/1‑94 (MQ=255)
                                                atcTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACtt                                          >  2:7652324/1‑94 (MQ=255)
                                                atcTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACtt                                          <  1:1492688/94‑1 (MQ=255)
                                                 tctcTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACttt                                         <  2:9824460/94‑1 (MQ=255)
                                                 gctctTTAAAGGGCGCTTGTTTGATACCCCCGAGGGGGCCGGCGAGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTTCAGCAACgtt                                         <  1:5520579/93‑1 (MQ=255)
                                                  ctctTCAAAGGTCGCTTTTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTg                                        <  2:2959151/94‑1 (MQ=255)
                                                  ctctTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTg                                        >  1:2804578/1‑94 (MQ=255)
                                                  ctctTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTg                                        >  2:2093393/1‑94 (MQ=255)
                                                   tctTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGt                                       >  1:3184020/1‑94 (MQ=255)
                                                    ctTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTc                                      >  1:6364981/1‑94 (MQ=255)
                                                     ttCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCa                                     >  1:10145580/1‑94 (MQ=255)
                                                     ttCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGACGGTTACAGCAACTTTGTCa                                     >  2:7127968/1‑94 (MQ=255)
                                                      tCAAAGGTCGCTTGTTTGATCCCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAg                                    <  2:7981923/94‑1 (MQ=255)
                                                       cAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGc                                   >  2:10554017/1‑94 (MQ=255)
                                                       cAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGc                                   >  1:2657435/1‑94 (MQ=255)
                                                        aaaGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCg                                  <  2:6937505/94‑1 (MQ=255)
                                                        aaaGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCg                                  <  2:6427134/94‑1 (MQ=255)
                                                        aaaGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCg                                  <  2:4585301/94‑1 (MQ=255)
                                                          aGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGtt                                >  2:7735849/1‑94 (MQ=255)
                                                           ggTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTTAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTc                               <  1:5150434/94‑1 (MQ=255)
                                                           ggTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTc                               >  1:5863699/1‑94 (MQ=255)
                                                            gTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCa                              <  1:1248994/94‑1 (MQ=255)
                                                            gTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCa                              <  1:4679345/94‑1 (MQ=255)
                                                            gTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCa                              <  1:9694432/94‑1 (MQ=255)
                                                               gCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGaa                           <  2:6016967/94‑1 (MQ=255)
                                                               gCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGaa                           <  2:10399286/94‑1 (MQ=255)
                                                                cTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCCACTTTGTCAGCGTTCAGAAc                          >  1:4499073/1‑94 (MQ=255)
                                                                 ttGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAACg                         <  1:4892341/94‑1 (MQ=255)
                                                                 ttGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAACg                         >  2:1085884/1‑94 (MQ=255)
                                                                  tGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAACga                        >  2:8725120/1‑94 (MQ=255)
                                                                  tGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAACga                        >  1:74063/1‑94 (MQ=255)
                                                                   gTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAACgat                       >  1:7505135/1‑94 (MQ=255)
                                                                   gTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAACgat                       <  1:3403782/94‑1 (MQ=255)
                                                                   gTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAACgat                       >  1:5015629/1‑94 (MQ=255)
                                                                    tttGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAACgatg                      <  2:1251003/94‑1 (MQ=255)
                                                                      tGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAACgatgat                    <  2:8813082/94‑1 (MQ=255)
                                                                      tGAGACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAACgatgat                    <  2:1601223/94‑1 (MQ=255)
                                                                         tACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAACGATGATGTa                 >  1:7914802/1‑94 (MQ=255)
                                                                         tACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAACGATGATGTa                 <  2:9578073/94‑1 (MQ=255)
                                                                          accaccGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAACGATGATGTaa                <  2:5529736/94‑1 (MQ=255)
                                                                           ccaccGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAACGATGATGTAAt               >  2:10115677/1‑94 (MQ=255)
                                                                            caccGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAACGATGATGTAATc              <  2:312595/94‑1 (MQ=255)
                                                                             accGATATGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAACGATGATGTAATCa             >  2:5341279/1‑94 (MQ=255)
                                                                              ccGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAACGATGATGTAATCAc            >  2:6865537/1‑94 (MQ=255)
                                                                               cGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAACGATGATGTAATCAcc           >  2:9017759/1‑94 (MQ=255)
                                                                                gATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAACGATGATGTAATCACCg          >  1:9226000/1‑94 (MQ=255)
                                                                                 aTGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAACGATGATGTAATCACCgg         >  2:2438549/1‑94 (MQ=255)
                                                                                      gCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAACGATGATGTAATCACCGGTATCt    <  1:2078344/94‑1 (MQ=255)
                                                                                       ccGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAACGATGATGTAATCACCGGTATCTa   <  1:6115811/94‑1 (MQ=255)
                                                                                       ccGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAACGATGATGTAATCACCGGTATCTa   >  1:6843801/1‑94 (MQ=255)
                                                                                       ccGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAACGATGATGTAATCACCGGTATCTa   <  2:5116698/94‑1 (MQ=255)
                                                                                        cGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAACGATGATGTAATCACCGGTATCTAc  >  1:9971784/1‑94 (MQ=255)
                                                                                           |                                                                                          
ATGCCTTTAACCGCGATTTCAATCACACGCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGTGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAACGATGATGTAATCACCGGTATCTAC  >  NC_000913/3375964‑3376145

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGG  >  NC_000913/3389719‑3389905
                                                                                             |                                                                                             
CGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCG                                                                                               >  SRR4302545.2882977/1‑94 (MQ=60)
 GGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGG                                                                                              >  SRR4302545.412897/1‑94 (MQ=60)
 GGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGG                                                                                              >  SRR4302545.5654363/1‑94 (MQ=60)
 GGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGG                                                                                              >  SRR4302545.2185596/1‑94 (MQ=60)
 GGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGG                                                                                              >  SRR4302545.6345269/1‑94 (MQ=60)
 GGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGG                                                                                              >  SRR4302545.7124107/1‑94 (MQ=60)
 GGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGG                                                                                              <  SRR4302545.7152937/94‑1 (MQ=60)
 GGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGG                                                                                              <  SRR4302545.1247870/94‑1 (MQ=60)
 GGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGG                                                                                              <  SRR4302545.7763296/94‑1 (MQ=60)
  GCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGC                                                                                            <  SRR4302545.9760050/94‑1 (MQ=60)
  GCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGC                                                                                            <  SRR4302545.9760049/94‑1 (MQ=60)
   CGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCG                                                                                           >  SRR4302545.997690/1‑94 (MQ=60)
   CGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCG                                                                                           <  SRR4302545.2841939/94‑1 (MQ=60)
   CGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCG                                                                                           <  SRR4302545.5019222/94‑1 (MQ=60)
   CGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCG                                                                                           <  SRR4302545.10691564/94‑1 (MQ=60)
    GTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGT                                                                                          <  SRR4302545.10781672/94‑1 (MQ=60)
    GTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGT                                                                                          >  SRR4302545.3795355/1‑94 (MQ=60)
     TGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGTA                                                                                         >  SRR4302545.1450209/1‑94 (MQ=57)
      GAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGTAA                                                                                        >  SRR4302545.3461983/1‑94 (MQ=51)
      GAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGTAA                                                                                        >  SRR4302545.3653854/1‑94 (MQ=51)
      GAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGTAA                                                                                        >  SRR4302545.10299136/1‑94 (MQ=51)
      GAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGTAA                                                                                        >  SRR4302545.8410609/1‑94 (MQ=51)
       AACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGTATG‑CGGCGTAAA                                                                                       >  SRR4302545.1361145/1‑94 (MQ=47)
       AACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGTAAA                                                                                       >  SRR4302545.7263153/1‑94 (MQ=49)
       AACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGTAAA                                                                                       >  SRR4302545.5125033/1‑94 (MQ=49)
        ACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGTAAAC                                                                                      <  SRR4302545.7526303/94‑1 (MQ=46)
        ACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGTAAAC                                                                                      <  SRR4302545.3772998/94‑1 (MQ=46)
        ACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGTAAAC                                                                                      >  SRR4302545.4518706/1‑94 (MQ=46)
        ACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGTAAAC                                                                                      >  SRR4302545.6252365/1‑94 (MQ=46)
        ACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGTAAAC                                                                                      <  SRR4302545.1063388/94‑1 (MQ=46)
        ACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGTAAAC                                                                                      <  SRR4302545.7317933/94‑1 (MQ=46)
        ACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGTAAAC                                                                                      <  SRR4302545.6496989/94‑1 (MQ=46)
        ACGCACTATCGAGCCTATGGTTATGTTGCGTTTTGTAGGCCTGATAAGACCCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGTAAAC                                                                                      <  SRR4302545.7258695/94‑1 (MQ=4)
         CGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGTANACG                                                                                     >  SRR4302545.8905596/1‑94 (MQ=43)
         CGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGTAAACG                                                                                     <  SRR4302545.10690722/94‑1 (MQ=44)
           CCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGTAAACGCC                                                                                   >  SRR4302545.2287633/1‑94 (MQ=39)
           CCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGTAAACGCC                                                                                   >  SRR4302545.648480/1‑94 (MQ=39)
           CCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGTAAACGCC                                                                                   >  SRR4302545.8786774/1‑94 (MQ=39)
            CCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGTAAACGCCT                                                                                  >  SRR4302545.73826/1‑94 (MQ=37)
            CCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGTAAACGCCT                                                                                  <  SRR4302545.361979/94‑1 (MQ=37)
            CCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGTAAACGCCT                                                                                  >  SRR4302545.5688175/1‑94 (MQ=37)
            CCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGTAAACGCCT                                                                                  >  SRR4302545.6134136/1‑94 (MQ=37)
             CTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGTCAACGCCTT                                                                                 >  SRR4302545.7877400/1‑94 (MQ=33)
             CTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGTAAACGCCTT                                                                                 <  SRR4302545.10155445/94‑1 (MQ=34)
             CTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGTAAACGCCTT                                                                                 >  SRR4302545.8628976/1‑94 (MQ=34)
             CTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGTAAACGCCTT                                                                                 >  SRR4302545.5894268/1‑94 (MQ=34)
              TATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGTAAACGCCTTA                                                                                >  SRR4302545.9164317/1‑94 (MQ=32)
              TATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGTAAACGCCTTA                                                                                <  SRR4302545.7156552/94‑1 (MQ=32)
               ATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGTACACGCCTTAT                                                                               >  SRR4302545.1964057/1‑94 (MQ=28)
               ATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGTAAACGCCTTAT                                                                               >  SRR4302545.4736204/1‑94 (MQ=29)
               ATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGTAAACGCCTTAT                                                                               <  SRR4302545.1027322/94‑1 (MQ=29)
               ATCCAGCCTACGGATATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGCTG‑GGGCGTAAACGCCGTAT                                                                               >  SRR4302545.1964056/1‑94 (MQ=27)
                TCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGTAAACGCCTTATC                                                                              <  SRR4302545.1376819/94‑1 (MQ=27)
                TCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGTAAACGCCTTATC                                                                              >  SRR4302545.5904376/1‑94 (MQ=27)
                TCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGTAAACGCCTTATC                                                                              >  SRR4302545.6543454/1‑94 (MQ=27)
                TCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGTAAACGCCTTATC                                                                              <  SRR4302545.3943450/94‑1 (MQ=27)
                TCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGTAAACGCCTTATC                                                                              >  SRR4302545.2922703/1‑94 (MQ=27)
                TCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGTAAACGCCTTATC                                                                              <  SRR4302545.10547349/94‑1 (MQ=27)
                 CCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGTAAACGCCTTATCC                                                                             <  SRR4302545.2910773/94‑1 (MQ=24)
                 CCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGTAAACGCCTTATCC                                                                             >  SRR4302545.6932465/1‑94 (MQ=24)
                 CCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGTAAACGCCTTATCC                                                                             <  SRR4302545.6693010/94‑1 (MQ=24)
                 CCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGTAAACGCCTTATCC                                                                             >  SRR4302545.6289016/1‑94 (MQ=24)
                 CCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGTAAACGCCTTATCC                                                                             <  SRR4302545.8395546/94‑1 (MQ=24)
                 CCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGTAAACGCCTTATCC                                                                             <  SRR4302545.958595/94‑1 (MQ=24)
                 CCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGTAAACGCCTTATCC                                                                             <  SRR4302545.10083882/94‑1 (MQ=24)
                 CCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGTAAACGCCTTATCA                                                                             <  SRR4302545.7271550/94‑1 (MQ=24)
                  CAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGTAAACGCCTTATCCG                                                                            >  SRR4302545.5722294/1‑94 (MQ=22)
                  CAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGTAAACGCCTTATCCG                                                                            <  SRR4302545.5710183/94‑1 (MQ=22)
                  CAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGTAAACGCCTTATCCG                                                                            >  SRR4302545.4884926/1‑94 (MQ=22)
                  CAGCCTACGGTTATGTGCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGTAAACGCCTTATCCG                                                                            <  SRR4302545.1533265/94‑1 (MQ=21)
                   AGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGTAAACGCCTTATCCGA                                                                           <  SRR4302545.4700260/94‑1 (MQ=20)
                   AGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGTAAACGCCTTATCCGA                                                                           >  SRR4302545.3759147/1‑94 (MQ=20)
                   AGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGTAAACGCCTTATCCGA                                                                           >  SRR4302545.10877924/1‑94 (MQ=20)
                   AGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGTAAACGCCTTATCCGA                                                                           >  SRR4302545.7478330/1‑94 (MQ=20)
                   AGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGTAAACGCCTTATCCGA                                                                           >  SRR4302545.4242109/1‑94 (MQ=20)
                   AGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGTAAACGCCTTATCCGA                                                                           >  SRR4302545.2581295/1‑94 (MQ=20)
                   AGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGTAAACGCCTTATCCGA                                                                           >  SRR4302545.4321785/1‑94 (MQ=20)
                    GCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGTAAACGCCTTATCCGAC                                                                          <  SRR4302545.3319957/94‑1 (MQ=14)
                    GCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGTAAACGCCTTATCCGAC                                                                          >  SRR4302545.9031677/1‑94 (MQ=14)
                     CCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGTAAACGCCTTATCCGACC                                                                         <  SRR4302545.7580084/94‑1 (MQ=12)
                     CCTACGGTTATGTTCCGGTTTGTAGATCTGATAAGACGCACAGCCTCGCATCAGCCTACGGCTGTCGGACGCC‑GCGTAAACGCCCTATCCGACT                                                                         >  SRR4302545.5377341/1‑94 (MQ=57)
                      CTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGTAAACGCCTTATCCGACCT                                                                        >  SRR4302545.10901648/1‑94 (MQ=12)
                      CTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGTAAACGCCTTATCCGACCT                                                                        <  SRR4302545.2200508/94‑1 (MQ=12)
                      CTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGTAAACGCCTTATCCGACCT                                                                        >  SRR4302545.2879506/1‑94 (MQ=12)
                      CTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGTAAACGCCTTATCCGACCT                                                                        >  SRR4302545.6994795/1‑94 (MQ=12)
                      CTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGTAAACGCCTTATCCGACCT                                                                        <  SRR4302545.684300/94‑1 (MQ=12)
                       ttcgatgATGTTCCGATTTGCAGGCGTGATAAGACGCACAGCGTCGCATCAGTCAACGGCTGTCGGAT‑TCGGCGTAAACTCCTTATCCGACCTA                                                                       <  SRR4302545.7568073/87‑1 (MQ=0)
                       TACGGTTATGTTCCGTTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGTAAACGCCTTATCCGACCTA                                                                       <  SRR4302545.10705425/94‑1 (MQ=0)
                       TACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGTAAACGCCTTATCCGACCTA                                                                       >  SRR4302545.1186685/1‑94 (MQ=12)
                       TACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGTAAACGCCTTATCCGACCTA                                                                       <  SRR4302545.1742768/94‑1 (MQ=12)
                        CCGGTTATGTTCCGGTTTGTAGGCCTGATAAGCCGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGTAAACGCCTTATCCGACCTAC                                                                      <  SRR4302545.4452051/94‑1 (MQ=11)
                        ACTGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCTCATCAGGCAACGGCTGTCGGATG‑CGGCGTAAACGCCTTATCCGACCTAC                                                                      <  SRR4302545.4452053/94‑1 (MQ=11)
                        ACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGTAAACGCCTTATCCGACCTAC                                                                      <  SRR4302545.4452052/94‑1 (MQ=12)
                        ACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGTAAACGCCTTATCCGACCTAC                                                                      <  SRR4302545.4922805/94‑1 (MQ=12)
                         CGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGTAAACGCCTTATCCGACCTACG                                                                     <  SRR4302545.5464151/94‑1 (MQ=12)
                         CGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGTAAACGCCTTATCCGACCTACG                                                                     >  SRR4302545.7300233/1‑94 (MQ=12)
                         CGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGTAAACGCCTTATCCGACCTACG                                                                     >  SRR4302545.9963712/1‑94 (MQ=12)
                         CGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGTAAACGCCTTATCCGACCTACG                                                                     >  SRR4302545.3021860/1‑94 (MQ=12)
                         CGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGTAAACGCCTTATCCGACCTACG                                                                     <  SRR4302545.7229162/94‑1 (MQ=12)
                         CGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGTAAACGCCTTATCCGACCTACG                                                                     <  SRR4302545.8171854/94‑1 (MQ=12)
                         CGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGTAAACGCCTTATCCGACCTACG                                                                     <  SRR4302545.8171855/94‑1 (MQ=12)
                         CGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGTAAACGCCTTATCCGACCTACG                                                                     <  SRR4302545.4419660/94‑1 (MQ=12)
                          GGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGTAAACGCCTTATCCGACCTACGG                                                                    <  SRR4302545.196463/94‑1 (MQ=12)
                          GGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGTAAACGCCTTATCCGACCTACGG                                                                    <  SRR4302545.6453744/94‑1 (MQ=12)
                           GTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGTAAACGCCTTATCCGACCTACGGT                                                                   >  SRR4302545.8483067/1‑94 (MQ=12)
                           GTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGTAAACGCCTTATCCGACCTACGGT                                                                   <  SRR4302545.3067944/94‑1 (MQ=12)
                            TTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGTAAACGCCTTATCCGACCTACGGTT                                                                  <  SRR4302545.4578611/94‑1 (MQ=12)
                            TTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGTAAACGCCTTATCCGACCTACGGTT                                                                  <  SRR4302545.2931526/94‑1 (MQ=12)
                            TTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGTAAACGCCTTATCCGACCTACGGTT                                                                  <  SRR4302545.4578612/94‑1 (MQ=12)
                            TTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGTAAACGCCTTATCCGACCTACGGTT                                                                  <  SRR4302545.7136455/94‑1 (MQ=12)
                            TTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGTAAACGCCTTATCCGACCTACGGTT                                                                  >  SRR4302545.776576/1‑94 (MQ=12)
                            TTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGTAAACGCCTTATCCGACCTACGGCT                                                                  >  SRR4302545.6319784/1‑94 (MQ=11)
                            TTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CAGCGTAAACGCCTTATCCGACCTACGGTT                                                                  >  SRR4302545.9413559/1‑94 (MQ=11)
                            TTATGTTCCGGTTTGTAAGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGTAAACGCCTTATCCGACCTACGGTT                                                                  >  SRR4302545.10335225/1‑94 (MQ=11)
                            GTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGTAAACGCCTTATCCGACCTACGGTT                                                                  <  SRR4302545.1437468/94‑1 (MQ=12)
                             TATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGTAAACGCCTTATCCGACCTACGGTTA                                                                 >  SRR4302545.8678110/1‑94 (MQ=12)
                             TATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGTAAACGCCTTATCCGACCTACGGTTA                                                                 >  SRR4302545.4094316/1‑94 (MQ=12)
                              ATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGTAAACGCCTTATCCGACCTACGGTTAT                                                                >  SRR4302545.1245427/1‑94 (MQ=12)
                              ATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGTAAACGCCTTATCCGACCTACGGTTAT                                                                >  SRR4302545.1259094/1‑94 (MQ=12)
                              ATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGTAAACGCCTTATCCGACCTACGGTTAT                                                                >  SRR4302545.4536920/1‑94 (MQ=12)
                              ATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGTAAACGCCTTATCCGACCTACGGTTAT                                                                <  SRR4302545.3753511/94‑1 (MQ=12)
                              ATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGTAAACGCCTTATCCGACCTACGGTTAT                                                                >  SRR4302545.4459513/1‑94 (MQ=12)
                              ATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGTAAACGCCTTATCCGACCTACGGTTAT                                                                >  SRR4302545.4459512/1‑94 (MQ=12)
                               TGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGTAAACGCCTTATCCGACCTACGGTTATG                                                               >  SRR4302545.5586983/1‑94 (MQ=9)
                               TGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGTAAACGCCTTATCCGACCTACGGTTATG                                                               <  SRR4302545.2636797/94‑1 (MQ=9)
                               TGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGTAAACGCCTTATCCGACCTACGGTTATG                                                               <  SRR4302545.8606437/94‑1 (MQ=9)
                                GTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGTAAACGCCTTATCCGAGCTACGGATATGT                                                              >  SRR4302545.5002224/1‑94 (MQ=6)
                                GTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGTAAACGCCTTATCCGACCTACGGTTATGT                                                              >  SRR4302545.7280199/1‑94 (MQ=7)
                                GTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGTAAACGCCTTATCCGACCTACGGTTATGT                                                              >  SRR4302545.1336636/1‑94 (MQ=7)
                                GTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGTAAACGCCTTATCCGACCTACGGTTATGT                                                              >  SRR4302545.5832490/1‑94 (MQ=7)
                                GTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGTAAACGCCTTATCCGACCTACGGTTATGT                                                              >  SRR4302545.9400259/1‑94 (MQ=7)
                                 TTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGTAAACGCCTTATCCGACCTACGGTTATGTT                                                             <  SRR4302545.4693299/94‑1 (MQ=4)
                                  TCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGTAAACGCCTTATCCGACCTACGGTTATGTTC                                                            <  SRR4302545.3353847/94‑1 (MQ=2)
                                  TCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGTAAACGCCTTATCCGACCTACGGTTATGTTC                                                            <  SRR4302545.6845907/94‑1 (MQ=2)
                                   CCGGTTTGTAGGCCTGATAAGACTCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCC                                                           <  SRR4302545.7463420/94‑1 (MQ=0)
                                   CCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCC                                                           <  SRR4302545.1108638/94‑1 (MQ=0)
                                   CCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCC                                                           >  SRR4302545.6253635/1‑94 (MQ=0)
                                   CCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCC                                                           >  SRR4302545.10299514/1‑94 (MQ=0)
                                   CCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCC                                                           <  SRR4302545.6981097/94‑1 (MQ=0)
                                   CCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCC                                                           <  SRR4302545.10261191/94‑1 (MQ=0)
                                   CCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCC                                                           <  SRR4302545.4216588/94‑1 (MQ=0)
                                   CCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGTAAACGCCTTATCCGACCTACGGGTATGTTCC                                                           >  SRR4302545.10299727/1‑94 (MQ=0)
                                    CGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATG‑CGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCG                                                          <  SRR4302545.2329746/94‑1 (MQ=0)
                                                                                             CGGCGTAATCGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGG  <  SRR4302545.5206196/94‑1 (MQ=0)
                                                                                             CGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCGACGGCTACGAGATGCGG  >  SRR4302545.8281541/1‑94 (MQ=0)
                                                                                             CGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGG  <  SRR4302545.820353/94‑1 (MQ=0)
                                                                                             CGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGG  <  SRR4302545.6956534/94‑1 (MQ=0)
                                                                                             |                                                                                             
CGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGG  >  NC_000913/3389719‑3389905

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑