Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 647 270.1 21258240 98.4% 20918108 94.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,450,175 T→C Q130R (CAG→CGG)  rplC ← 50S ribosomal subunit protein L3

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,450,1750TC99.2% 2005.8 / 2.0 586Q130R (CAG→CGG) rplC50S ribosomal subunit protein L3
Reads supporting (aligned to +/- strand):  ref base T (3/2);  new base C (246/335);  total (249/337)
Fisher's exact test for biased strand distribution p-value = 6.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.30e-01

Only 100 of 612 total aligned reads displayed.

TTTCTTGCCTTTGAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTTTAACGTCAGCAAACA  >  NC_000913/3450084‑3450265
                                                                                           |                                                                                          
  tCTTGCCTTTGAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGt                                                                                        <  2:7680711/94‑1 (MQ=255)
   cTTGCCTTTGAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTa                                                                                       >  2:3357997/1‑94 (MQ=255)
     tGCCTTTGAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACg                                                                                     <  1:1197103/94‑1 (MQ=255)
     tGCCTTTGAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACg                                                                                     <  2:8767548/94‑1 (MQ=255)
       ccTTTGAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGa                                                                                   >  2:6560686/1‑94 (MQ=255)
        cTTTGAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGaa                                                                                  <  2:8008845/94‑1 (MQ=255)
        cTTTGAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGaa                                                                                  >  2:809482/1‑94 (MQ=255)
        cTTTGAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGaa                                                                                  >  1:8016808/1‑94 (MQ=255)
         tttGAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAg                                                                                 <  2:4707971/94‑1 (MQ=255)
         tttGAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAg                                                                                 <  1:5910817/94‑1 (MQ=255)
         tttGAACACTTGGCCCGGAGTCTGGTTCTGAACTATAGAACCCGGAAGGCTGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAg                                                                                 <  2:5651727/94‑1 (MQ=255)
               cgcTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAg                                                                           <  1:3624888/92‑1 (MQ=255)
               cacTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAg                                                                           <  2:8372091/94‑1 (MQ=255)
                acTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAgc                                                                          >  2:785401/1‑94 (MQ=255)
                acTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAgc                                                                          <  2:1372838/94‑1 (MQ=255)
                   ttGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCt                                                                       <  1:7353963/94‑1 (MQ=255)
                   ttGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCt                                                                       >  2:9136899/1‑94 (MQ=255)
                    tGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCtt                                                                      >  2:5051705/1‑94 (MQ=255)
                     gCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTa                                                                     >  1:7007203/1‑94 (MQ=255)
                       ccGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAAc                                                                   >  2:719599/1‑94 (MQ=255)
                       ccGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAAc                                                                   >  2:936987/1‑94 (MQ=255)
                       ccGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAAc                                                                   >  2:5804843/1‑94 (MQ=255)
                         ggAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGACGTTACAGCGCTTAAAgg                                                                 >  1:1456785/1‑94 (MQ=255)
                         ggAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACgg                                                                 <  2:10100597/94‑1 (MQ=255)
                          gAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGt                                                                <  2:1649820/94‑1 (MQ=255)
                           aGTCTGGTTCTGACCGATAGAAGCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTa                                                               >  2:1840427/1‑94 (MQ=255)
                           aGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTa                                                               >  2:844251/1‑94 (MQ=255)
                            gTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTAc                                                              >  1:1849069/1‑94 (MQ=255)
                            gTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTAc                                                              >  2:5352435/1‑94 (MQ=255)
                            gTCTGGTTCTGACCGATAGAACCCGGAACGCGGAGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGCAGATCCAGCGCTTAACGGTAc                                                              >  2:10490819/1‑94 (MQ=255)
                             tCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTAcc                                                             <  2:4410767/94‑1 (MQ=255)
                             tCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTAca                                                             <  1:2555571/94‑2 (MQ=255)
                              cTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCt                                                            >  1:8576892/1‑94 (MQ=255)
                                ggTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGc                                                          <  1:579426/94‑1 (MQ=255)
                                ggTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGc                                                          >  1:8281730/1‑94 (MQ=255)
                                 gTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCg                                                         >  2:668357/1‑94 (MQ=255)
                                   tCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGaa                                                       >  2:2487347/1‑94 (MQ=255)
                                    cTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGaac                                                      >  2:9577063/1‑93 (MQ=255)
                                      gACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAacc                                                    >  1:4692371/1‑94 (MQ=255)
                                      gACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAacc                                                    >  2:3288045/1‑94 (MQ=255)
                                        ccGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAacctt                                                  <  2:10162119/94‑1 (MQ=255)
                                        ccGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAacctt                                                  >  2:6348009/1‑94 (MQ=255)
                                        ccGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAacctt                                                  >  2:1755446/1‑94 (MQ=255)
                                         cGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAaccttt                                                 >  1:4824475/1‑94 (MQ=255)
                                         cGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAaccttt                                                 <  1:1271130/94‑1 (MQ=255)
                                          gATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAaccttta                                                <  2:2914602/94‑1 (MQ=255)
                                          gATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAaccttta                                                >  1:932499/1‑94 (MQ=255)
                                          gATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAaccttta                                                >  1:2379669/1‑94 (MQ=255)
                                           aTAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAacctttac                                               <  1:9906439/94‑1 (MQ=255)
                                           aTAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAacctttac                                               <  1:2841227/94‑1 (MQ=255)
                                           aTAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAacctttac                                               <  1:1656107/94‑1 (MQ=255)
                                            tAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAacctttacc                                              >  1:9753119/1‑94 (MQ=255)
                                            tAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAacctttacc                                              <  2:155197/94‑1 (MQ=255)
                                              gAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAacctttacctt                                            >  2:4192449/1‑94 (MQ=255)
                                                aCCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAAcctttaccttta                                          <  1:6024707/94‑1 (MQ=255)
                                                 cccGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTag                                         >  1:4301089/1‑94 (MQ=255)
                                                 cccGGAAAGCGGGTAGACAAGGAATTACCGTCAGTAGCGTCCCGGCTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTag                                         <  2:7967202/94‑1 (MQ=255)
                                                  ccGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTaga                                        <  2:9790457/94‑1 (MQ=255)
                                                   cGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTagag                                       <  1:10007147/94‑1 (MQ=255)
                                                    ggAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAgg                                      <  2:1095135/94‑1 (MQ=255)
                                                    ggAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAgg                                      <  1:3064019/94‑1 (MQ=255)
                                                      aaCGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTg                                    <  1:10237028/94‑1 (MQ=255)
                                                         gcgGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCa                                 <  1:2117530/94‑1 (MQ=255)
                                                         gcgGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCa                                 <  1:5232305/94‑1 (MQ=255)
                                                         gcgGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCa                                 <  2:685070/94‑1 (MQ=255)
                                                          cgGTGAGACAAGGAGTGACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAg                                <  1:7717039/94‑1 (MQ=255)
                                                            gTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGtt                              <  2:8245260/94‑1 (MQ=255)
                                                             tGAGACAGGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTa                             >  1:7561744/1‑94 (MQ=255)
                                                             tGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTa                             <  1:395054/94‑1 (MQ=255)
                                                              gagaCAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTAc                            <  2:2664385/94‑1 (MQ=255)
                                                              gagaCAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTAc                            <  2:3945794/94‑1 (MQ=255)
                                                               agaCAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACg                           >  1:10390810/1‑94 (MQ=255)
                                                               agaCAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACg                           >  1:415976/1‑94 (MQ=255)
                                                               agaCAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACg                           >  1:3863506/1‑94 (MQ=255)
                                                                 aCAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTATCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTc                         <  1:1766982/94‑1 (MQ=255)
                                                                  cAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCa                        >  1:2221145/1‑94 (MQ=255)
                                                                   aaGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCaa                       >  2:5578356/1‑94 (MQ=255)
                                                                    aGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAAc                      >  1:9508081/1‑94 (MQ=255)
                                                                     ggAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACt                     <  2:623790/94‑1 (MQ=255)
                                                                        gTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACtttt                  >  2:8586791/1‑94 (MQ=255)
                                                                        gTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACtttt                  <  1:5421940/94‑1 (MQ=255)
                                                                         ttACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACttttt                 <  2:9700896/94‑1 (MQ=255)
                                                                           aCCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTTTa               >  2:3140330/1‑94 (MQ=255)
                                                                            ccGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTTTaa              <  2:7461927/94‑1 (MQ=255)
                                                                            ccGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTTTaa              >  1:876536/1‑94 (MQ=255)
                                                                             cGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTTTAAc             <  1:3361554/94‑1 (MQ=255)
                                                                             cGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTTTAAc             >  1:4502653/1‑94 (MQ=255)
                                                                             cGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTTTAAc             <  1:6537109/94‑1 (MQ=255)
                                                                              gTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTTTAACg            >  2:3662344/1‑94 (MQ=255)
                                                                               tGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTTTAACGt           <  2:1958856/94‑1 (MQ=255)
                                                                               tGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTTTAACGt           <  1:6352681/94‑1 (MQ=255)
                                                                                gAGTAGCGTCCTGGGGACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTTTAACGTc          >  2:5208334/1‑94 (MQ=255)
                                                                                gAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTTTAACGTc          <  1:6699135/94‑1 (MQ=255)
                                                                                 aGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTTTAACGTCa         <  1:2947731/94‑1 (MQ=255)
                                                                                 aGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTTTAACGTCa         >  2:89096/1‑94 (MQ=255)
                                                                                 aGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTTTAACGTCa         <  1:1970675/94‑1 (MQ=255)
                                                                                      cGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTTTAACGTCAGCaaa    <  2:10364223/94‑1 (MQ=255)
                                                                                       gTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTTTAACGTCAGCAAAc   <  1:8998004/94‑1 (MQ=255)
                                                                                       gTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTTTAACGTCAGCAAAc   >  2:6079242/1‑94 (MQ=255)
                                                                                        tCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTTTAACGTCAGCAAACa  <  1:9085389/94‑1 (MQ=255)
                                                                                           |                                                                                          
TTTCTTGCCTTTGAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTTTAACGTCAGCAAACA  >  NC_000913/3450084‑3450265

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 266 total aligned reads displayed.
CAATGCCGACAATTCACGCCCGTATCACCCCAACAAGCGCAGATGTTTAAATATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCTGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCATGGCGATCCACGAAATGATGGTGGTGACGCCGGAATTACGGGCCGCTATTCATGAAA  >  NC_000913/3457125‑3457311
                                                                                             |                                                                                             
CAATGCCGACAATTCACGCCCGTATCACCCCAACAAGCGCAGATGTTTAAATATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCC                                                                                               <  SRR4302545.3386770/94‑1 (MQ=60)
CAATGCCGACAATTCACGCCCGTATCACCCCAACAAGCGCAGATGTTTAAATATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCC                                                                                               >  SRR4302545.5336209/1‑94 (MQ=60)
 AATGCCGACAATTCACGCCCGTATCACCCCAACAAGCGCAGATGTTTAAATATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCCG                                                                                              >  SRR4302545.480198/1‑94 (MQ=60)
  ATGCCGACAATTCACGCCCGTATCACCCCAACAAGCGCAGATGTTTAAATATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCCGC                                                                                             >  SRR4302545.4012521/1‑94 (MQ=60)
   TGCCGACAATTCACGCCCGTATCACCCCAACAAGCGCAGATGTTTAAATATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCCGCC                                                                                            <  SRR4302545.880519/94‑1 (MQ=60)
   TGCCGACAATTCACGCCCGTATCACCCCAACAAGCGCAGATGTTTAAATATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCCGCC                                                                                            >  SRR4302545.5844668/1‑94 (MQ=60)
     CCGACAATTCACGCCCGTATCACCCCAACAAGCGCAGATGTTTAAATATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCCGCCCT                                                                                          <  SRR4302545.8122900/94‑1 (MQ=60)
      CGACAATTCACGCCCGTATCACCCCAACAAGCGCAGATGTTTAAATATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCCGCCCTC                                                                                         >  SRR4302545.9852156/1‑94 (MQ=60)
      CGACAATTCACGCCCGTATCACCCCAACAAGCGCAGATGTTTAAATATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCCGCCCTC                                                                                         <  SRR4302545.6593553/94‑1 (MQ=60)
      CGACAATTCACGCCCGTATCACCCCAACAAGCGCAGATGTTTAAATATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCCGCCCTC                                                                                         >  SRR4302545.9736877/1‑94 (MQ=60)
       GACAATTCACGCCCGTATCACCCCAACAAGCGCAGATGTTTAAATATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCCCCCCTCA                                                                                        >  SRR4302545.1638171/1‑94 (MQ=60)
         CAATTCACGCCCGTATCACCCCAACAAGCGCAGATGTTTAAATATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATT                                                                                      >  SRR4302545.79324/1‑94 (MQ=60)
         CAATTCACGCCCGTATCACCCCAACAAGCGCAGATGTTTAAATATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATT                                                                                      <  SRR4302545.6411556/94‑1 (MQ=60)
         CAATTCACGCCCGTATCACCCCAACAAGCGCAGATGTTTAAATATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATT                                                                                      <  SRR4302545.1109985/94‑1 (MQ=60)
          AATTCACGCCCGTATCACCCCAACAAGCGCAGATGTTTAAATATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATTG                                                                                     >  SRR4302545.6206893/1‑94 (MQ=60)
          AATTCACGCCCGTATCACCCCAACAAGCGCAGATGTTTAAATATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATTG                                                                                     <  SRR4302545.10108664/94‑1 (MQ=60)
           ATTCACGCCCGTATCACCCCAACAAGCGCAGATGTTTAAATATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATTGC                                                                                    >  SRR4302545.555462/1‑94 (MQ=60)
           ATTCACGCCCGTATCACCCCAACAAGCGCAGATGTTTAAATATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATTGC                                                                                    >  SRR4302545.1737236/1‑94 (MQ=60)
           ATTCACGCCCGTATCACCCCAACAAGCGCAGATGTTTAAATATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATTGC                                                                                    >  SRR4302545.4015932/1‑94 (MQ=60)
             tcacggccgtagctgaCCAACAAGGGGAGATGGTTAAATATCATCAGCGCGCGGTGACAACAATTGGCACTCCCGTAGGCTGCCCTCATTGCCA                                                                                  <  SRR4302545.9425978/78‑1 (MQ=60)
              CACGCCCGTATCACCCCAACAAGCGCAGATGTTTAAATATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCAT                                                                                 >  SRR4302545.8715742/1‑94 (MQ=60)
              CACGCCCGTATCACCCCAACAAGCGCAGATGTTTAAATATCATCAACTCGACGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCAT                                                                                 >  SRR4302545.392676/1‑94 (MQ=60)
               CCGCCCGTATCACCCCAACAAGCGCAGATGTTTAAATATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATC                                                                                <  SRR4302545.8442515/94‑1 (MQ=60)
                 GCCCGTATCACCCCAACAAGCGCAGATGTTTAAATATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAA                                                                              <  SRR4302545.3209424/94‑1 (MQ=60)
                 GCCCGTATCACCCCAACAAGCGCAGATGTTTAAATATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAA                                                                              >  SRR4302545.9752862/1‑94 (MQ=60)
                 GCCCGTATCACCCCAACAAGCGCAGATGTTTAAATATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAA                                                                              <  SRR4302545.6149964/94‑1 (MQ=60)
                 GCCCGTATCACCCCAACAAGCGCAGATGTTTAAATATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATTGACATCAA                                                                              >  SRR4302545.4802731/1‑94 (MQ=60)
                  CCCGTATCACCCCAACAAGCGCAGATGTTTAAATATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAAT                                                                             >  SRR4302545.4940909/1‑94 (MQ=60)
                   CCGTATCACCCCAACAAGCGCAGATGTTTAAATATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATC                                                                            <  SRR4302545.3509450/94‑1 (MQ=60)
                   CCGTATCACCCCAACAAGCGCAGATGTTTAAATATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATC                                                                            <  SRR4302545.10048216/94‑1 (MQ=60)
                    CGTATCACCCCAACAAGCGCAGATGTTTAAATATCATCAGCTCGCGGTGACAACAATTGCCACTCCCGTGGGCCGCCCTCATTGCCATCAATCC                                                                           >  SRR4302545.2845840/1‑94 (MQ=60)
                     GTATCACCCCAACAAGCGCAGATGTTTAAATATCATCAGCTCGCGGTGACAACAATTTGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCG                                                                          >  SRR4302545.8347952/1‑94 (MQ=60)
                     GTATCACCCCAACAAGCGCAGATGTTTAAATATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCG                                                                          >  SRR4302545.4610520/1‑94 (MQ=60)
                     GTATCACCCCAACAAGCGCAGATGTTTAAATATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCG                                                                          >  SRR4302545.10091146/1‑94 (MQ=60)
                     GTATCACCCCAACAAGCGCAGATGTTTAAATATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCG                                                                          >  SRR4302545.2726251/1‑94 (MQ=60)
                     GTATCACCCCAACAAGCGCAGATGTTTAAATATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCG                                                                          <  SRR4302545.10781184/94‑1 (MQ=60)
                     GTATCACCCCAACAAGCGCAGATGTTTAAATATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCG                                                                          >  SRR4302545.7092507/1‑94 (MQ=60)
                      TATCACCCCAACAAGCGCAGATGTTTAAATATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGG                                                                         >  SRR4302545.3857431/1‑94 (MQ=60)
                       ATCACCCCAACAAGCGCAGATGTTTAAATATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGC                                                                        >  SRR4302545.5431513/1‑94 (MQ=60)
                       ATCACCCCAACAAGCGCAGATGTTGAAATATCATCAGCTCGCGGTGACAACAATTGGCTCTCCCGTAGGCCGTCCTCATTGCCATCAATCCGGC                                                                        <  SRR4302545.272189/94‑1 (MQ=60)
                        TCACCCCAACAAGCGCAGATGTTTAAATATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCT                                                                       >  SRR4302545.747003/1‑94 (MQ=60)
                        TCACCCCAACAAGCGCAGATGTTTAAATATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCT                                                                       >  SRR4302545.28819/1‑94 (MQ=60)
                         CACCCCAACAAGCGCAGATGTTTAAATATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTA                                                                      <  SRR4302545.8596361/94‑1 (MQ=60)
                          ACCCCAACAAGCGCAGATGTTTAAATATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTAT                                                                     <  SRR4302545.5982193/94‑1 (MQ=60)
                           CCCCAACAAGCGCAGATGTTTAAATATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATC                                                                    >  SRR4302545.5476583/1‑94 (MQ=60)
                            CCCCACAAGCGCAGGTGTTTAAATATCATCATCTCGCGGTGACAACAATTGGCTCTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCA                                                                   <  SRR4302545.7465587/94‑1 (MQ=60)
                            CCCAACAAGCGCAGATGTTTAAATATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCA                                                                   <  SRR4302545.1082694/94‑1 (MQ=60)
                            CCCAACAAGCGCAGATGTTTAAATATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCA                                                                   <  SRR4302545.311461/94‑1 (MQ=60)
                            CCCAACAAGCGCAGATGTTTAAATATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCA                                                                   <  SRR4302545.2051088/94‑1 (MQ=60)
                            CCCAACAAGCGCAGATGTTTAAATATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCA                                                                   <  SRR4302545.6762240/94‑1 (MQ=60)
                            CCCAACAAGCGCAGATGTTTAAATATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCA                                                                   >  SRR4302545.6998106/1‑94 (MQ=60)
                            CCCAACAAGCGCAGATGTTTAAATATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCA                                                                   >  SRR4302545.7268878/1‑94 (MQ=60)
                            CCCAACAAGCGCAGATGTTTAAATATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCA                                                                   <  SRR4302545.9731505/94‑1 (MQ=60)
                             CCAACAAGCGCAGATGTTTAAATATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCAG                                                                  >  SRR4302545.10741514/1‑94 (MQ=60)
                             CCAACAAGCGCAGATGTTTAAATATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCAG                                                                  <  SRR4302545.5624731/94‑1 (MQ=60)
                             CCAACAAGCGCAGATGTTTAAATATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCAG                                                                  <  SRR4302545.8908322/94‑1 (MQ=60)
                             CCAACAAGCGCAGATGTTTAAATATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGGAGGCCGCCCTCATTGCCATCAATCCGGCTATCAG                                                                  >  SRR4302545.6805496/1‑94 (MQ=60)
                              CAACAAGCGCAGATGTTTAAATATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCAGG                                                                 >  SRR4302545.5343247/1‑94 (MQ=60)
                              CAACAAGCGCAGATGTTTAAATATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCAGG                                                                 <  SRR4302545.10036183/94‑1 (MQ=60)
                              CAACAAGCGCAGATGTTTAAATATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCAGG                                                                 >  SRR4302545.2877705/1‑94 (MQ=60)
                              CAACAAGCGCAGATGTTTAAATATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCAGG                                                                 >  SRR4302545.2221312/1‑94 (MQ=60)
                               AACAAGCGCAGATGTTTAAATATCATCATCTCGCGGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCAGGG                                                                <  SRR4302545.8201057/94‑1 (MQ=60)
                               AACAAGCGCAGATGTTTAAATATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCAGGG                                                                <  SRR4302545.2996269/94‑1 (MQ=60)
                               AACAAGCGCAGATGTTTAAATATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCAGGG                                                                >  SRR4302545.8575228/1‑94 (MQ=60)
                               AACAAGCGCAGATGTTTAAATATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCAGGG                                                                >  SRR4302545.3070521/1‑94 (MQ=60)
                                ACAAGCGCAGATGTTTAAATATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCAGGGG                                                               >  SRR4302545.3180101/1‑94 (MQ=60)
                                ACAAGCGCAGATGTTTAAATATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCAGGGG                                                               <  SRR4302545.1671425/94‑1 (MQ=60)
                                 CAAGCGCAGATGTTTAAATATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCAGGGGC                                                              <  SRR4302545.6283808/94‑1 (MQ=60)
                                  AAGCGCAGATGTTTAAATATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCAGGGGCG                                                             <  SRR4302545.8248568/94‑1 (MQ=60)
                                  AAGCGCAGATGTTTAAATATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCAGGGGCG                                                             <  SRR4302545.4595955/94‑1 (MQ=60)
                                  AAGCGCAGATGTTTAAATATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCAGGGGCG                                                             >  SRR4302545.7830325/1‑94 (MQ=60)
                                  AAGAGCAGATGTTTAAATATCATCATCTCGCGGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCAGGGGCG                                                             <  SRR4302545.4595956/94‑1 (MQ=60)
                                   AGCGCAGATGTTTAAATATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGC                                                            >  SRR4302545.5384481/1‑94 (MQ=60)
                                    GCGCAGATGTTTAAATATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCA                                                           >  SRR4302545.2153487/1‑94 (MQ=60)
                                    GCGCAGATGTTTAAATATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCA                                                           <  SRR4302545.3406938/94‑1 (MQ=60)
                                     CGCAGATGTTTAAATATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCAT                                                          <  SRR4302545.5175027/94‑1 (MQ=60)
                                     CGCAGATGTTTAAATATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCAT                                                          <  SRR4302545.873286/94‑1 (MQ=60)
                                      GCAGATGTTTAAATATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCATG                                                         <  SRR4302545.6534797/94‑1 (MQ=60)
                                      GCAGATGTTTAAATATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCATG                                                         >  SRR4302545.10472917/1‑94 (MQ=60)
                                      GCAGATGTTTAAATATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCATG                                                         <  SRR4302545.8726761/94‑1 (MQ=60)
                                       CAGATGTTTAAATATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCATGG                                                        <  SRR4302545.7718617/94‑1 (MQ=60)
                                       CAGATGTTTAAATATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCATGG                                                        >  SRR4302545.1957331/1‑94 (MQ=60)
                                       CAGATGTTTAAATATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCATGG                                                        >  SRR4302545.9029017/1‑94 (MQ=60)
                                       CAGATGTTTAAATATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCATGG                                                        >  SRR4302545.9029018/1‑94 (MQ=60)
                                        AGATGTTTAAATATCATCAGGTCGCGGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCATGGC                                                       <  SRR4302545.5423633/94‑1 (MQ=60)
                                        AGATGTTTAAATATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCATGGC                                                       >  SRR4302545.4256404/1‑94 (MQ=60)
                                        AGATGTTTAAATATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCATGGC                                                       >  SRR4302545.2215323/1‑94 (MQ=60)
                                        AGATGTTTAAATATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCATGGC                                                       >  SRR4302545.2380771/1‑94 (MQ=60)
                                        AGATGTTTAAATATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCATGGC                                                       >  SRR4302545.4941583/1‑94 (MQ=60)
                                        AGATGTTTAAATATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCAGGGC                                                       >  SRR4302545.9029823/1‑94 (MQ=60)
                                         GATGTTTCAATATCATCAGCTCGCGGTGACAACAATAGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCATGGCG                                                      <  SRR4302545.9501239/94‑1 (MQ=60)
                                           TGTTTAAATATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCATGGCGAT                                                    >  SRR4302545.8619568/1‑94 (MQ=60)
                                           TGTTTAAATATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCATGGCGAT                                                    >  SRR4302545.6172486/1‑94 (MQ=60)
                                            GTTTAAATATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGTCTATCCGGGGCGCAGGGCGATC                                                   >  SRR4302545.9417521/1‑94 (MQ=60)
                                            GTTTAAATATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCATGGCGATC                                                   >  SRR4302545.9417519/1‑94 (MQ=60)
                                            GTTTAAATATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCATGGCGATC                                                   >  SRR4302545.9702710/1‑94 (MQ=60)
                                            GTTTAAATATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCATGGCGATC                                                   >  SRR4302545.10632907/1‑94 (MQ=60)
                                            GTTTAAATATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCATGGCGATC                                                   >  SRR4302545.2569590/1‑94 (MQ=60)
                                             TTTAAATATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCATGGCGATCC                                                  <  SRR4302545.9731262/94‑1 (MQ=60)
                                             TTTAAATATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCCG‑CCTCATTGCCATCAATCCGGCTATCAGGGGCGCATGGCGATCCA                                                 <  SRR4302545.8333200/94‑1 (MQ=60)
                                              TTAAATATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCATGGCGATCCA                                                 >  SRR4302545.5644695/1‑94 (MQ=60)
                                              TTAAATATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCATGGCGATCCA                                                 <  SRR4302545.7010273/94‑1 (MQ=60)
                                              TTAAATATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCATGGCGATCCA                                                 <  SRR4302545.2579758/94‑1 (MQ=60)
                                              TTAAATATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCAGGCTATCAGGGGCGCATGGCGCTCCA                                                 >  SRR4302545.6335973/1‑94 (MQ=60)
                                               TAAATATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCATGGCGATCCAC                                                <  SRR4302545.9797235/94‑1 (MQ=60)
                                               TAAATATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCATGGCGATCCAC                                                >  SRR4302545.5617850/1‑94 (MQ=60)
                                                AAATATCATCAGCTCGCGGTGCCAACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTTTCAGGGGCGCATTGCGATCCACG                                               >  SRR4302545.2023092/1‑94 (MQ=60)
                                                AAATATCATCAGCTCGCGGTGACGACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCATGGCGATCCACG                                               >  SRR4302545.6369286/1‑94 (MQ=60)
                                                AAATATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCATGGCGCTCCACG                                               <  SRR4302545.10833776/94‑1 (MQ=60)
                                                AAATATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCATGGCGATCCACG                                               >  SRR4302545.3401854/1‑94 (MQ=60)
                                                AAATATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCATGGCGATCCACG                                               <  SRR4302545.6911277/94‑1 (MQ=60)
                                                AAATATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCATGGCGATCCACG                                               >  SRR4302545.4844285/1‑94 (MQ=60)
                                                 AATATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCATGGCGATCCACGA                                              >  SRR4302545.3563571/1‑94 (MQ=60)
                                                 AATATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCATGGCGATCCACGA                                              >  SRR4302545.4476817/1‑94 (MQ=60)
                                                  ATATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCATGGCGATCCACGCA                                             >  SRR4302545.10068397/1‑94 (MQ=60)
                                                   AATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCATGGCGATCCACGAAA                                            <  SRR4302545.8578312/94‑1 (MQ=60)
                                                    ATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCATGGCGATCCACGAAAT                                           <  SRR4302545.8292690/94‑1 (MQ=60)
                                                    ATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCATGGCGATCCACGAAAT                                           <  SRR4302545.1355446/94‑1 (MQ=60)
                                                    ATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCATGGCGATCCACGAAAT                                           <  SRR4302545.58939/94‑1 (MQ=60)
                                                    ATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCATGGCGATCCACGAAAT                                           >  SRR4302545.5771301/1‑94 (MQ=60)
                                                      CATCAGCTCGCGGTGACAACAATTGGCAGTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCATGGCGATCCACGAAATGA                                         <  SRR4302545.9289021/94‑1 (MQ=60)
                                                      CATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCCGACCTCATTGCCATCAATCCGGCTATCAGTGGCGCATGGCGATCCACGAAATGA                                         >  SRR4302545.6770868/1‑94 (MQ=60)
                                                       ATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCATGGCGATCCACGAAATGAT                                        <  SRR4302545.787531/94‑1 (MQ=60)
                                                       ATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCATGGCGATCCACGAAATGAT                                        <  SRR4302545.2453740/94‑1 (MQ=60)
                                                       ATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCATGGCGATCCACGAAATGAT                                        <  SRR4302545.2162692/94‑1 (MQ=60)
                                                        TCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCATGGCGATCCACGAAATGATG                                       >  SRR4302545.7619526/1‑94 (MQ=60)
                                                        TCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCATGGCGATCCACGAAATGATG                                       <  SRR4302545.2391952/94‑1 (MQ=60)
                                                         CAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCATGGCGATCCACGAAATGATGG                                      >  SRR4302545.194530/1‑94 (MQ=60)
                                                          AGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGAATGGCGATCCACGAAATGATGGT                                     >  SRR4302545.2448010/1‑94 (MQ=60)
                                                           GCTCGCGGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCATGGCGATCCACGAAATGATGGTG                                    >  SRR4302545.4709926/1‑94 (MQ=60)
                                                            CTCGCGGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCATGGCGATCCACGAAATGATGGTGG                                   <  SRR4302545.8407802/94‑1 (MQ=60)
                                                            CTCGCGGTGACAACAATTGGCACTACCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCATGGCGATCCACGAAATGATGGTGG                                   <  SRR4302545.5735593/94‑1 (MQ=60)
                                                             TCGCGGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCATGGCGATCCACGAAATGATGGTGGT                                  <  SRR4302545.66165/94‑1 (MQ=60)
                                                             GCGCGGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCACTCCGGCTATCAGGGGCGCATGGCGATCCACGAAATGATGGTGGT                                  <  SRR4302545.1109861/94‑1 (MQ=60)
                                                              CGCGGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCATGGCGATCCACGAAATGATGGTGGTG                                 <  SRR4302545.8357918/94‑1 (MQ=60)
                                                              CGCGGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCATGGCGATCCACGAAATGATGGTGGTG                                 >  SRR4302545.6734735/1‑94 (MQ=60)
                                                               GCGGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCATGGCGATCCACGAAATGATGGTGGTGA                                <  SRR4302545.10103988/94‑1 (MQ=60)
                                                               GCGGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCATGGCGATCCACGAAATGATGGTGGTGA                                <  SRR4302545.886622/94‑1 (MQ=60)
                                                               GCGGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCATGGCGATCCACGAAATGATGGTGGTGA                                >  SRR4302545.8646714/1‑94 (MQ=60)
                                                               GCGGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCATGGCGATCCACGAAATGATGGTGGTGA                                >  SRR4302545.413682/1‑94 (MQ=60)
                                                                CGGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCATGGCGATCCACGAAATGATGGTGGTGAC                               >  SRR4302545.1274044/1‑94 (MQ=60)
                                                                CGGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCATGGCGATCCACGAAATGATGGTGGTGAC                               <  SRR4302545.9790073/94‑1 (MQ=60)
                                                                 GGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCATGGCGATCCACGAAATGATGGTGGTGACG                              >  SRR4302545.9719472/1‑94 (MQ=60)
                                                                 GGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCATGGCGATCCACGAAATGATGGTGGTGACG                              >  SRR4302545.2878803/1‑94 (MQ=60)
                                                                 GGTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCATGGCGATCCACGAAATGATGGTGGTGACG                              <  SRR4302545.311547/94‑1 (MQ=60)
                                                                  GTGACAACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCATGGCGATCCACGAAATGATGGTGGTGACGC                             >  SRR4302545.151815/1‑94 (MQ=60)
                                                                   TGACAACAATTGGCACTCCCGTAGGCTGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCATGGCGATCCACGAAATGATGGTGGTGACGCC                            >  SRR4302545.10338426/1‑94 (MQ=60)
                                                                   TGACAACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCATGGCGATCCACGAAATGATGGTGGTGACGCC                            <  SRR4302545.8790452/94‑1 (MQ=60)
                                                                     CCAACAATTGGGCCTCGCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCATGGCGATCCACGAAATGATGGTGGTGACGCCGG                          <  SRR4302545.8554305/94‑1 (MQ=60)
                                                                     ACAACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCATGGCGATCCACGAAATGATGGTGGTGACGCCGG                          <  SRR4302545.10481519/94‑1 (MQ=60)
                                                                      CAACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCATGGCGATCCACGAAATGATGGTGGTGACGCCGGA                         <  SRR4302545.9547944/94‑1 (MQ=60)
                                                                      CAACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCATGGCGATCCACGAAATGATGGTGGTGACGCCGGA                         <  SRR4302545.9547945/94‑1 (MQ=60)
                                                                      CAACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAAACCGGCTATCAGGGGCGCATGGCGATCCACGAAATGATGGTGGTGACGCCGGA                         >  SRR4302545.5740488/1‑94 (MQ=60)
                                                                       AACACTTGGCAAACCCGTAGGCTGACCTCCTTGCCATTAATCCGGCTATCAGGGGCGCATGGCGATCCACGAAATGATGGAGGTGACGCCGGgt                        >  SRR4302545.6671323/1‑92 (MQ=60)
                                                                       AACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCATGGCGATCCACGAAATGATGGTGGTGACGCCGGAA                        >  SRR4302545.8750122/1‑94 (MQ=60)
                                                                       AACAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCATGGCGATCCACGAAATGATGGTGGTGACGCCGGAA                        >  SRR4302545.5083224/1‑94 (MQ=60)
                                                                         CAATTGGCACTCCCGTAGGCTGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCATGGCGATCCACGAAATGATGGTGGTGACGCCGGAATT                      >  SRR4302545.7180991/1‑94 (MQ=60)
                                                                         CAATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCATGGCGATCCACGAAATGATGGTGGTGACGCCGGAATT                      <  SRR4302545.10399365/94‑1 (MQ=60)
                                                                          AATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCATGGCGATCCACGAAATGATGGTGGTGACGCCGGAATTA                     >  SRR4302545.8635972/1‑94 (MQ=60)
                                                                           ATTGGCGCGCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCATGGCGATCCACGAAATGATGGTGGTGACGCCGGAATTAC                    <  SRR4302545.7499724/94‑1 (MQ=60)
                                                                           ATTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCATGGCGATCCACGAAAGGATGGTGGTGACGCCGGAATTAC                    <  SRR4302545.10540127/94‑1 (MQ=60)
                                                                            TTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCATGGCGATCCACGAAATGATGGTGGTGACGCCGGAATTACG                   <  SRR4302545.10893812/94‑1 (MQ=60)
                                                                            TTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCATGGCGATCCACGAAATGATGGTGGTGACGCCGGAATTACG                   >  SRR4302545.4989976/1‑94 (MQ=60)
                                                                            TTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCATGGCGATCCACGAAATGATGGTGGTGACGCCGGAATTACG                   <  SRR4302545.3986187/94‑1 (MQ=60)
                                                                            TTGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCAGGGCCGCATGGCGATCCACGAAATGATGGTGGTGACGCCGGAATTACG                   <  SRR4302545.4078431/94‑1 (MQ=60)
                                                                             TGGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCATGGCGATCCACGAAATGATGGTGGTGACGCCGGAATTACGG                  >  SRR4302545.8552518/1‑94 (MQ=60)
                                                                              GGCACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCATGGCGATCCACGAAATGATGGTGGTGACGCCGGAATTACGGG                 <  SRR4302545.10249298/94‑1 (MQ=60)
                                                                                CACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCATGGCGATCCACGAAATGATGGTGGTGACGCCGGAATTACGGGCC               <  SRR4302545.3952498/94‑1 (MQ=60)
                                                                                CACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCATGGCGATCCACGAAATGATGGTGGTGACGCCGGAATTACGGGCC               <  SRR4302545.2674052/94‑1 (MQ=60)
                                                                                CACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCATGGCGATCCACGAAATGATGGTGGTGACGCCGGAATTACGGGCC               <  SRR4302545.10874632/94‑1 (MQ=60)
                                                                                CACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCATGGCGATCCACGAAATGATGGTGGTGACGCCGGAATTACGGGCC               >  SRR4302545.352781/1‑94 (MQ=60)
                                                                                CACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCATGGCGATCCACGAAATGATGGTGGTGACGCCGGAATTACGGGCC               >  SRR4302545.3148725/1‑94 (MQ=60)
                                                                                CACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCATGGCGATCCACGAAATGATGGTGGTGACGCCGGAATTACGGGCC               <  SRR4302545.3871958/94‑1 (MQ=60)
                                                                                 CCTCCCGTAGGCCGCCCCCATTGCCATCAATCCGGCTATCAGGGGCGCATGGCGATCCACGAAATGATGGTGGTGACGCCGGAATTACGGGCCG              <  SRR4302545.7109886/94‑1 (MQ=60)
                                                                                 ACTCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCATGGCGATCCACGAAATGATGGTGGTGACGCCGGAATTACGGGCCG              >  SRR4302545.3596768/1‑94 (MQ=60)
                                                                                   TCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCATGGCGATCCACGAAATGATGGTGGTGACGCCGGAATTACGGGCCGCT            <  SRR4302545.6201202/94‑1 (MQ=60)
                                                                                   TCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCATGGCGATCCACGAAATGATGGTGGTGACGCCGGAATTACGGGCCGCT            <  SRR4302545.4970771/94‑1 (MQ=60)
                                                                                   TCCCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCATGGCGATCCACGAAATGATGGTGGTGACGCCGGAATTACGGGCCGCT            <  SRR4302545.7298725/94‑1 (MQ=60)
                                                                                   TCCCGTAGGCCGCCCTCATTGCCAGCAATCCGGCTATCAGGGACGCATGGCGATCCACGAAATGATGGTGGTGACGCCGGAATTACGGGCCGCT            <  SRR4302545.4151595/94‑1 (MQ=60)
                                                                                    CCCGTAGGCCGCCCTCATTGCCATCAATCCAGCTATCAGGGGCGCATGGCGATCCACGAAATGATGGTGGTGACGCCGGAATTACGGGCCGCTA           >  SRR4302545.9179126/1‑94 (MQ=60)
                                                                                     CCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCATGGCGATCCACGAAATGATGGTGGTGACGCCGGAATTACGGGCCGCTAT          <  SRR4302545.8870738/94‑1 (MQ=60)
                                                                                     CCGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCATGGCGATCCACGAAATGATGGTGGTGACGCCGGAATTACGGGCCGCTAT          <  SRR4302545.4277298/94‑1 (MQ=60)
                                                                                      CGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCATGGCGATCCACGAAATGATGGTGGTGACGCCGGAATTACGGGCCGCTATT         >  SRR4302545.5895633/1‑94 (MQ=60)
                                                                                      CGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCATGGCGATCCACGAAATGATGGTGGTGACGCCGGAATTACGGGCCGCTATT         <  SRR4302545.10064077/94‑1 (MQ=60)
                                                                                      CGTAGGCCGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCATGGCGATCCACGAAATGATGGTGGTGACGCCGGAATTACGGGCCGCTATT         >  SRR4302545.8468649/1‑94 (MQ=60)
                                                                                       GTAGGCCGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCATGGCGATCCACGAAATGATGGTGGTGACGCCGGAATTACGGGCCGCTATTC        >  SRR4302545.3545986/1‑94 (MQ=60)
                                                                                       CTAGGCCGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCATGGCGATCCACGAAATGATGGTGGTGACGCCGGAATTACGGGCCGCTATTC        <  SRR4302545.8542777/94‑1 (MQ=60)
                                                                                        TATGCAGCCCTCCTTGCCATCAATCCGGCTATCAGGGGCGCATGGCGATCTCAGAAATGATGGTTGTGACGCCGGATTCACGGGTCGCTATTGA       >  SRR4302545.5539417/1‑94 (MQ=46)
                                                                                        TAGGCCGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCATGGCGATCCACGAAATGATGGTGGTGACGCCGGAATTACGGGCCGCTATTCA       >  SRR4302545.5539416/1‑94 (MQ=60)
                                                                                        TAGGCCGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCATGGCGATCCACGAAATGATGGTGGTGACGCCGGAATTACGGGCCGCTATTCA       >  SRR4302545.235987/1‑94 (MQ=60)
                                                                                        TAGCCCGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCATGGCGATCCACGAAATGATGGTGGTGACGCCGGAATTACGGGCCGCTATTCA       <  SRR4302545.9060310/94‑1 (MQ=60)
                                                                                         AGGCCGCCCTCATTGCCATCATTCCGGCTATCAGGGGCGAATGGCGATCCACGAAATGATGGTGGTGACGCCGGAATTACGGGCCGCTATTCAT      <  SRR4302545.4655562/94‑1 (MQ=60)
                                                                                         AGGCCGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCATGGCGATCCACGAAATGATGGTGGTGACGCCGGAATTACGGGCCGCTATTCAT      <  SRR4302545.5442168/94‑1 (MQ=60)
                                                                                         AGGCCGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCATGGCGATCCACGAAATGATGGTGGTGACGCCGGAATTACGGGCCGCTATTCAT      >  SRR4302545.7809480/1‑94 (MQ=60)
                                                                                         AGGCCGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCATGGCGATCCACGAAATGATGGTGGTGACGCCGGAATTACGGGCCGCTATTCAT      >  SRR4302545.667367/1‑94 (MQ=60)
                                                                                          GGCCGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCATGGCGATCCACGAAATGATGGTGGTGACGCCGGAATTACGGGCCGCTATTCATG     <  SRR4302545.2977483/94‑1 (MQ=60)
                                                                                          GGCCGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCATGGCGATCCACGAAATGATGGTGGTGACGCCGGAATTACGGGCCGCTATTCATG     <  SRR4302545.2977484/94‑1 (MQ=60)
                                                                                          GGCCGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCATGGCGATCCACGAAATGATGGTGGTGACGCCGGAATTACGGGCCGCTATTCATG     <  SRR4302545.2526758/94‑1 (MQ=60)
                                                                                            CCGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCATGGCGATCCACGAAATGATGGTGGTGACGCCGGAATTACGGGCCGCTATTCATGAA   <  SRR4302545.4241147/94‑1 (MQ=60)
                                                                                            CCGCCCGCATTGCCATCAATCCGGCTATCAGGGGCGCATGGCGATCCGCGAAATGATGGTGGTGACGCCGGAATTACGGGCCGCTATTCATGAA   <  SRR4302545.2236006/94‑1 (MQ=60)
                                                                                             |                                                                                             
CAATGCCGACAATTCACGCCCGTATCACCCCAACAAGCGCAGATGTTTAAATATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCTGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCATGGCGATCCACGAAATGATGGTGGTGACGCCGGAATTACGGGCCGCTATTCATGAAA  >  NC_000913/3457125‑3457311

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑