Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 647 270.1 21258240 98.4% 20918108 94.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,510,121 +G intergenic (‑358/+151) yhfZ ← / ← trpS hypothetical protein/tryptophan tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,510,1201.G96.8% 1936.1 / 43.7 570intergenic (‑357/+152)yhfZ/trpShypothetical protein/tryptophan tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base . (8/10);  new base G (312/240);  total (320/250)
Fisher's exact test for biased strand distribution p-value = 3.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

Only 100 of 661 total aligned reads displayed.

GAAGATAAGGCGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCC‑GTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAGGC  >  NC_000913/3510027‑3510210
                                                                                              |                                                                                          
gAAGATAAGGCGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTcccc                                                                                             <  1:9053367/94‑1 (MQ=255)
gAAGATAAGGCGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTcccc                                                                                             >  2:413270/1‑94 (MQ=255)
gAAGATAAGGCGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTcccc                                                                                             >  1:416338/1‑94 (MQ=255)
gAAGATAAGGCGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTcccc                                                                                             >  1:5635916/1‑94 (MQ=255)
gAAGATAAGGCGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTcccc                                                                                             <  2:10110757/94‑1 (MQ=255)
 aaGATAAGGCGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCC‑g                                                                                           >  2:4825322/1‑94 (MQ=255)
 aaGATAAGGCGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCC‑g                                                                                           >  2:4393820/1‑94 (MQ=255)
 aaGATAAGGCGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCC‑g                                                                                           >  2:2072553/1‑94 (MQ=255)
   gATAAGGCGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCC‑ggt                                                                                         >  2:2627843/1‑92 (MQ=255)
   gATAAGGCGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCC‑ggt                                                                                         >  1:5112665/1‑92 (MQ=255)
     tAAGGCGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCC‑Ggttt                                                                                       >  1:1081110/1‑93 (MQ=255)
      aaGGCGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCC‑Ggtttc                                                                                      >  2:3178491/1‑92 (MQ=255)
       aGGCGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCa                                                                                      <  2:3030582/94‑1 (MQ=255)
       aGGCGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCa                                                                                      >  1:1036182/1‑94 (MQ=255)
         gcgcAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCaga                                                                                    <  2:8628139/94‑1 (MQ=255)
          cgcAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCagag                                                                                   >  1:3194133/1‑94 (MQ=255)
          cgcAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCagag                                                                                   <  1:3384498/94‑1 (MQ=255)
          cgcAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCagag                                                                                   <  1:9733554/94‑1 (MQ=255)
          cgcAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCagag                                                                                   >  2:3906284/1‑94 (MQ=255)
             aaCAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGtga                                                                                >  2:199392/1‑94 (MQ=255)
             aaCAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGtga                                                                                >  1:9989381/1‑94 (MQ=255)
                aGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGtgatga                                                                             <  2:4571001/94‑1 (MQ=255)
                 gCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTgatgat                                                                            >  1:8676981/1‑94 (MQ=255)
                 gCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTgatgat                                                                            <  1:1317929/94‑1 (MQ=255)
                  cGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATa                                                                           >  1:4949368/1‑94 (MQ=255)
                  cGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATa                                                                           >  2:8192510/1‑94 (MQ=255)
                   gagaTGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATaa                                                                          >  2:649462/1‑94 (MQ=255)
                      aTGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAg                                                                       <  1:4457267/94‑1 (MQ=255)
                      aTGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAg                                                                       >  1:2189019/1‑94 (MQ=255)
                        gtgGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCa                                                                     >  2:1733302/1‑94 (MQ=255)
                            aaaCGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAAt                                                                 <  1:387467/94‑1 (MQ=255)
                            aaaCGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAAt                                                                 <  2:9882767/94‑1 (MQ=255)
                            aaaCGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAAt                                                                 >  1:6264428/1‑94 (MQ=255)
                             aaCGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAAtt                                                                <  1:8888103/94‑1 (MQ=255)
                             aaCGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAAtt                                                                <  1:616272/94‑1 (MQ=255)
                                ggCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGcc                                                             >  2:10519571/1‑94 (MQ=255)
                                ggCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGcc                                                             <  2:7380569/94‑1 (MQ=255)
                                ggCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGcc                                                             >  2:6235243/1‑94 (MQ=255)
                                  cGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTg                                                           >  1:7635728/1‑94 (MQ=255)
                                  cGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTg                                                           >  1:9586544/1‑94 (MQ=255)
                                   gAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGa                                                          >  2:9936637/1‑94 (MQ=255)
                                     ggCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATg                                                        <  2:9762630/94‑1 (MQ=255)
                                     ggCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATg                                                        <  2:9298443/94‑1 (MQ=255)
                                      gCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATgc                                                       <  2:9479395/94‑1 (MQ=255)
                                         cTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCt                                                    <  2:2837641/94‑1 (MQ=255)
                                         cTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCt                                                    <  1:1849480/94‑1 (MQ=255)
                                          ttCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTa                                                   >  2:7015918/1‑94 (MQ=255)
                                          ttCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTa                                                   <  1:8019639/94‑1 (MQ=255)
                                          ttCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTa                                                   <  2:839784/94‑1 (MQ=255)
                                          ttCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTa                                                   >  2:1432577/1‑94 (MQ=255)
                                           tCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTAc                                                  >  1:8347917/1‑94 (MQ=255)
                                           tCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTAc                                                  <  1:6011278/94‑1 (MQ=255)
                                           tCACATCTTTGCGGATTATGGCAAGTGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTAc                                                  <  2:10260207/94‑1 (MQ=38)
                                            cacaTTTTTTCGGATTATTGCAAATGAGGATAAAAACACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACg                                                 <  2:10616193/94‑1 (MQ=255)
                                            cacaTTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACg                                                 >  2:5703405/1‑94 (MQ=255)
                                             acaTTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGc                                                >  1:4738538/1‑94 (MQ=255)
                                                ttttttCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTa                                             >  1:5739200/1‑94 (MQ=255)
                                                 tttttCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTAt                                            >  2:7588669/1‑94 (MQ=255)
                                                 tttttCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTAt                                            <  2:5862987/94‑1 (MQ=255)
                                                 tttttCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTAt                                            >  1:4593579/1‑94 (MQ=255)
                                                   tttCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCa                                          <  1:5054683/94‑1 (MQ=255)
                                                   tttCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCa                                          <  2:3313984/94‑1 (MQ=255)
                                                    ttCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAg                                         <  1:8518627/94‑1 (MQ=255)
                                                    ttCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAg                                         >  2:6102071/1‑94 (MQ=255)
                                                    ttCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAg                                         >  1:484352/1‑94 (MQ=255)
                                                     tCGGATTATTGCAAATGAGGCTAAAAAAACCGGGTTTCCCCGGTTTCAGCGTGATGATAAAAGCAAAATTGCCTGATGCGCTCCGCTTATCAgg                                        >  2:8471418/1‑94 (MQ=37)
                                                         attattGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTa                                    <  1:9218672/94‑1 (MQ=255)
                                                            attACAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGAAGCGCTACGCTTATCAGGCCTACAt                                 >  1:7209140/1‑94 (MQ=25)
                                                              tGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACAttt                               >  1:10013428/1‑94 (MQ=255)
                                                               gCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGGTACGCTTATCAGGCCTACATTTc                              <  1:6724233/94‑1 (MQ=255)
                                                               gCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTc                              >  2:906437/1‑94 (MQ=255)
                                                                cAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTct                             >  1:3003185/1‑93 (MQ=25)
                                                                 aaaTGATGATAAAAAAGCCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCt                            <  1:2400207/94‑1 (MQ=25)
                                                                  aaTGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCtt                           <  2:6702784/94‑1 (MQ=255)
                                                                  aaTGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCtt                           >  1:10245770/1‑94 (MQ=255)
                                                                   aTGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTg                          >  2:7813071/1‑94 (MQ=255)
                                                                    tGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGc                         >  1:2533097/1‑94 (MQ=255)
                                                                      aGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCaa                       >  2:6833864/1‑94 (MQ=255)
                                                                       ggATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAat                      >  2:3417560/1‑94 (MQ=255)
                                                                       ggATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAat                      <  2:509051/94‑1 (MQ=255)
                                                                       ggATAAAAAAACCGGGGTTCCCCGGTTTCAGAGGGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAat                      >  2:1279771/1‑94 (MQ=25)
                                                                         aTAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAatat                    <  2:8002509/94‑1 (MQ=255)
                                                                         aTAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAatat                    <  1:2775890/94‑1 (MQ=255)
                                                                            aaaaaaCCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATAtgtg                 <  2:5607897/94‑1 (MQ=255)
                                                                            aaaaaaCCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATAtgtg                 >  1:6548826/1‑94 (MQ=255)
                                                                            aaaaaaCCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATAtgtg                 >  2:8877398/1‑94 (MQ=255)
                                                                              aaaaCCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCa               >  1:1570852/1‑94 (MQ=255)
                                                                              aaaaCCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCa               <  2:10403402/94‑1 (MQ=255)
                                                                               aaaCCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCAt              <  2:224688/94‑1 (MQ=255)
                                                                               aaaCCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCAt              <  2:1531863/94‑1 (MQ=255)
                                                                                 aCCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTa            >  2:5251108/1‑94 (MQ=255)
                                                                                  ccGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTAc           >  1:7798163/1‑94 (MQ=255)
                                                                                  ccGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTAc           >  2:7149375/1‑94 (MQ=255)
                                                                                   cGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACt          <  1:5563699/94‑1 (MQ=255)
                                                                                     ggTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACttt        <  1:7041419/94‑1 (MQ=255)
                                                                                       tttCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGt      <  1:3754634/94‑1 (MQ=255)
                                                                                       ttccccg‑GTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTa     <  1:5453804/91‑1 (MQ=255)
                                                                                        tccccg‑GTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAg    >  2:2426068/3‑94 (MQ=255)
                                                                                          cccg‑GTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAGGc  <  2:3698244/94‑1 (MQ=255)
                                                                                          cccg‑GTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAGGc  >  2:5393603/1‑94 (MQ=255)
                                                                                              |                                                                                          
GAAGATAAGGCGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCC‑GTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAGGC  >  NC_000913/3510027‑3510210

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 322 total aligned reads displayed.
GTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCG  >  NC_000913/3533016‑3533202
                                                                                             |                                                                                             
GTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGG                                                                                               <  SRR4302545.10133114/94‑1 (MQ=60)
GTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGG                                                                                               >  SRR4302545.7608079/1‑94 (MQ=60)
GTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGG                                                                                               >  SRR4302545.607868/1‑94 (MQ=60)
 TATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGAGACAGCCAGGTTTTCAGCCAGGC                                                                                              >  SRR4302545.4591321/1‑94 (MQ=60)
  ttggcgtaacgGCGGAGAGAAACCGCCGTGATGACTTTCTGTAAGCGGCACGCTTACCCAGATATTGGGCGACAGCCAGGTTGTCAGCCAGGCG                                                                                             <  SRR4302545.4525941/83‑1 (MQ=49)
  ATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCG                                                                                             >  SRR4302545.2639968/1‑94 (MQ=60)
  ATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCG                                                                                             >  SRR4302545.4819582/1‑94 (MQ=60)
  ATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCG                                                                                             <  SRR4302545.739806/94‑1 (MQ=60)
   TAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGA                                                                                            >  SRR4302545.8584539/1‑94 (MQ=60)
    AGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGAC                                                                                           >  SRR4302545.374469/1‑94 (MQ=60)
    AGCGGAACAGCGGAGAGAAATAGCCCTGATGAATTTCGGTCAGCGGCACGCGAACCCAGATATTGGGCGGCAGACAGGTTTTCAGCCAGGCGAC                                                                                           >  SRR4302545.3820388/1‑94 (MQ=60)
     GCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACA                                                                                          <  SRR4302545.3459630/94‑1 (MQ=60)
     GCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACA                                                                                          <  SRR4302545.540736/94‑1 (MQ=60)
      CGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAG                                                                                         >  SRR4302545.9227239/1‑94 (MQ=60)
       GGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGG                                                                                        >  SRR4302545.4300483/1‑94 (MQ=60)
       GGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGG                                                                                        >  SRR4302545.3032664/1‑94 (MQ=60)
       GGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGG                                                                                        <  SRR4302545.4389785/94‑1 (MQ=60)
        GAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGC                                                                                       >  SRR4302545.2973377/1‑94 (MQ=60)
        GAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGC                                                                                       <  SRR4302545.7315935/94‑1 (MQ=60)
         AACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCG                                                                                      <  SRR4302545.572360/94‑1 (MQ=60)
         AACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCG                                                                                      <  SRR4302545.885653/94‑1 (MQ=60)
         AACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGAGACAGGCG                                                                                      >  SRR4302545.3389647/1‑94 (MQ=60)
          ACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGA                                                                                     <  SRR4302545.2199664/94‑1 (MQ=60)
          ACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGA                                                                                     <  SRR4302545.2515959/94‑1 (MQ=60)
            AGCGGAGCGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAAC                                                                                   >  SRR4302545.10835977/1‑94 (MQ=60)
            AGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAAC                                                                                   >  SRR4302545.8086022/1‑94 (MQ=60)
            AGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAAC                                                                                   <  SRR4302545.4727680/94‑1 (MQ=60)
            AGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGGTTTCAGCCAGGCGACAGGCGAAC                                                                                   >  SRR4302545.8270992/1‑94 (MQ=60)
               GGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTT                                                                                <  SRR4302545.10723881/94‑1 (MQ=60)
               GGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTT                                                                                <  SRR4302545.683236/94‑1 (MQ=60)
               GGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTT                                                                                <  SRR4302545.10104857/94‑1 (MQ=60)
                GAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTT                                                                               <  SRR4302545.6590307/94‑1 (MQ=60)
                 AGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTG                                                                              <  SRR4302545.4002765/94‑1 (MQ=60)
                 AGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTG                                                                              >  SRR4302545.5297697/1‑94 (MQ=60)
                 AGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTG                                                                              >  SRR4302545.910814/1‑94 (MQ=60)
                 AGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTG                                                                              <  SRR4302545.145060/94‑1 (MQ=60)
                  GAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGT                                                                             <  SRR4302545.7080401/94‑1 (MQ=60)
                  GAGAAACCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGT                                                                             <  SRR4302545.2808374/94‑1 (MQ=60)
                   ATAAATCGCCCTGATTAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGGTTTCAGCCAGGCGACAGGCGAACTTTTGTT                                                                            <  SRR4302545.4662041/94‑1 (MQ=60)
                   AGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTT                                                                            >  SRR4302545.10438712/1‑94 (MQ=60)
                   AGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTT                                                                            <  SRR4302545.2771716/94‑1 (MQ=60)
                    GAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCTAGGCGACAGGCGAACTTTTGTTG                                                                           >  SRR4302545.7456302/1‑94 (MQ=60)
                    GAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTG                                                                           <  SRR4302545.1657109/94‑1 (MQ=60)
                    GAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCAAGGCGACAGGCGAACTTTTGTTG                                                                           >  SRR4302545.5992535/1‑94 (MQ=60)
                     AAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGA                                                                          >  SRR4302545.275824/1‑94 (MQ=60)
                     AAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGA                                                                          >  SRR4302545.6373246/1‑94 (MQ=60)
                      AATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGAC                                                                         <  SRR4302545.1840669/94‑1 (MQ=60)
                      AATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGAC                                                                         <  SRR4302545.690678/94‑1 (MQ=60)
                      AATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGAC                                                                         >  SRR4302545.351037/1‑94 (MQ=60)
                      AATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGAC                                                                         >  SRR4302545.2583991/1‑94 (MQ=60)
                       ATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACC                                                                        >  SRR4302545.8187610/1‑94 (MQ=60)
                        TCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCT                                                                       <  SRR4302545.7595095/94‑1 (MQ=60)
                        TCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCT                                                                       <  SRR4302545.10790006/94‑1 (MQ=60)
                         CGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTC                                                                      >  SRR4302545.2975650/1‑94 (MQ=60)
                         CGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTC                                                                      >  SRR4302545.9798306/1‑94 (MQ=60)
                          GCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCA                                                                     <  SRR4302545.1751294/94‑1 (MQ=60)
                          GCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCA                                                                     <  SRR4302545.1590903/94‑1 (MQ=60)
                          GCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCA                                                                     >  SRR4302545.2661180/1‑94 (MQ=60)
                           CCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAA                                                                    >  SRR4302545.3594889/1‑94 (MQ=60)
                            CCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAAC                                                                   <  SRR4302545.3691672/94‑1 (MQ=60)
                            CCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAAC                                                                   >  SRR4302545.4437433/1‑94 (MQ=60)
                            CCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAAC                                                                   <  SRR4302545.1648754/94‑1 (MQ=60)
                            CCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAAC                                                                   >  SRR4302545.5256313/1‑94 (MQ=60)
                            CCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGACGAACTTTTGTTGACCTCAAC                                                                   >  SRR4302545.8951651/1‑94 (MQ=60)
                              TGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGC                                                                 <  SRR4302545.2147790/94‑1 (MQ=60)
                              TGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGC                                                                 >  SRR4302545.324246/1‑94 (MQ=60)
                              TGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCAAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGC                                                                 >  SRR4302545.5354704/1‑94 (MQ=60)
                               GATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCG                                                                <  SRR4302545.6621209/94‑1 (MQ=60)
                                ATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGC                                                               <  SRR4302545.5834114/94‑1 (MQ=60)
                                 TGAATTTCTGTCTGCGGCTCGCGTACCCAGATATTGGGCGACAGCCAGGTTATCAGCCAGGCGACAGGCGAACTTTTGCTGACCTCAACGCGCA                                                              >  SRR4302545.9688750/1‑94 (MQ=60)
                                 TGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCA                                                              >  SRR4302545.10589733/1‑94 (MQ=60)
                                 TGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCA                                                              <  SRR4302545.1481668/94‑1 (MQ=60)
                                  GAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCAC                                                             >  SRR4302545.2532445/1‑94 (MQ=60)
                                  GAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCAC                                                             <  SRR4302545.10799125/94‑1 (MQ=60)
                                  GAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCAC                                                             >  SRR4302545.2459020/1‑94 (MQ=60)
                                    ATTTCGGTCAGCGGCCCGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGTCGAACTTTTGTTGACCTCAACGCGCACTT                                                           <  SRR4302545.3379718/94‑1 (MQ=60)
                                     TTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTC                                                          <  SRR4302545.1335700/94‑1 (MQ=60)
                                     TTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTC                                                          <  SRR4302545.9561167/94‑1 (MQ=60)
                                     TTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTC                                                          <  SRR4302545.6349840/94‑1 (MQ=60)
                                      TTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCC                                                         >  SRR4302545.7566276/1‑94 (MQ=60)
                                      TTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCC                                                         <  SRR4302545.7820126/94‑1 (MQ=60)
                                      TTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCC                                                         <  SRR4302545.7017058/94‑1 (MQ=60)
                                       TCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCG                                                        <  SRR4302545.9829076/94‑1 (MQ=60)
                                       TCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCG                                                        <  SRR4302545.8447167/94‑1 (MQ=60)
                                       TCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGATTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCG                                                        <  SRR4302545.5598460/94‑1 (MQ=60)
                                        CGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGT                                                       >  SRR4302545.8636405/1‑94 (MQ=60)
                                         GGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTC                                                      <  SRR4302545.8833828/94‑1 (MQ=60)
                                         GGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTC                                                      <  SRR4302545.5331758/94‑1 (MQ=60)
                                         GGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTC                                                      >  SRR4302545.1619325/1‑94 (MQ=60)
                                          GTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCG                                                     <  SRR4302545.5117495/94‑1 (MQ=60)
                                          GTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCG                                                     >  SRR4302545.7266261/1‑94 (MQ=60)
                                           TCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGG                                                    <  SRR4302545.3486285/94‑1 (MQ=60)
                                           TCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGG                                                    >  SRR4302545.6412196/1‑94 (MQ=60)
                                           TCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGG                                                    <  SRR4302545.8701077/94‑1 (MQ=60)
                                            CAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGG                                                   <  SRR4302545.7531207/94‑1 (MQ=60)
                                             AGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTGAACGCGCACTTCCGTCGGGC                                                  >  SRR4302545.1566804/1‑94 (MQ=60)
                                             AGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGC                                                  >  SRR4302545.9775733/1‑94 (MQ=60)
                                             AGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGC                                                  >  SRR4302545.4850068/1‑94 (MQ=60)
                                             AGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGC                                                  >  SRR4302545.1608759/1‑94 (MQ=60)
                                             AGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGC                                                  >  SRR4302545.4254030/1‑94 (MQ=60)
                                             AGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGC                                                  >  SRR4302545.10259378/1‑94 (MQ=60)
                                              GCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCC                                                 <  SRR4302545.1105301/94‑1 (MQ=60)
                                              GCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCC                                                 <  SRR4302545.1062134/94‑1 (MQ=60)
                                               TGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCG                                                >  SRR4302545.1090743/1‑94 (MQ=60)
                                               CGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCG                                                <  SRR4302545.122421/94‑1 (MQ=60)
                                                GGCACGCGTACCCATGTATTGGGTGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGC                                               <  SRR4302545.6079395/94‑1 (MQ=60)
                                                GGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGC                                               <  SRR4302545.9143608/94‑1 (MQ=60)
                                                GGCACGCGTACCCAGATATTGGCCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGC                                               <  SRR4302545.1813707/94‑1 (MQ=60)
                                                 GCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCC                                              >  SRR4302545.636405/1‑94 (MQ=60)
                                                  CACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCC                                             >  SRR4302545.9429337/1‑94 (MQ=60)
                                                  CACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCC                                             <  SRR4302545.5577145/94‑1 (MQ=60)
                                                  CACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCC                                             <  SRR4302545.1156862/94‑1 (MQ=60)
                                                  CACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTAACCTCAACGCGCACTTCCGTCGGGCCGCCC                                             <  SRR4302545.6709492/94‑1 (MQ=60)
                                                  CACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCACCCAGGCGACAACCGAACTTTTGTTGACCTCAACGAGCACTTCCGACGGGCCGCCT                                             >  SRR4302545.3282853/1‑94 (MQ=60)
                                                   ACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCA                                            >  SRR4302545.7293677/1‑94 (MQ=60)
                                                   ACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCA                                            >  SRR4302545.6012404/1‑94 (MQ=60)
                                                    CGCGTATCCATATAATGGGCGCCCGCCAGGTTTTCAGCCAGCCGACAGGCGAACTTATGTTGCCCTCACCGCGCACTTCCGTCGGGCCGCCCAG                                           <  SRR4302545.8327991/94‑1 (MQ=55)
                                                    CGCGTACTCAGATATTGGGCGACAGCCAGGCGTTCAGCCAGGCGACAGGCGGACTTTTGTTGACCTCAACGCGCATTTCCGGCGGGCCGCCCAG                                           >  SRR4302545.8948305/1‑94 (MQ=60)
                                                    CGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAG                                           >  SRR4302545.3651155/1‑94 (MQ=60)
                                                     GCGTACCCAGGTATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGT                                          <  SRR4302545.2427064/94‑1 (MQ=60)
                                                       GTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTG                                        >  SRR4302545.5820253/1‑94 (MQ=60)
                                                       GTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTG                                        >  SRR4302545.711213/1‑94 (MQ=60)
                                                        TACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGC                                       >  SRR4302545.6581580/1‑94 (MQ=60)
                                                        TACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCAAGTTGC                                       >  SRR4302545.2457913/1‑94 (MQ=60)
                                                         ACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCT                                      <  SRR4302545.8058187/94‑1 (MQ=60)
                                                          CCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTG                                     >  SRR4302545.4936218/1‑94 (MQ=60)
                                                          CCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTG                                     >  SRR4302545.4093601/1‑94 (MQ=60)
                                                          CCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTG                                     <  SRR4302545.10329506/94‑1 (MQ=60)
                                                          CCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTG                                     <  SRR4302545.5509696/94‑1 (MQ=60)
                                                          CCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTG                                     >  SRR4302545.3148103/1‑94 (MQ=60)
                                                           CCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGC                                    >  SRR4302545.9942515/1‑94 (MQ=60)
                                                           CCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGC                                    >  SRR4302545.6896541/1‑94 (MQ=60)
                                                           CCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGC                                    >  SRR4302545.1131836/1‑94 (MQ=60)
                                                           CCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGC                                    <  SRR4302545.3883646/94‑1 (MQ=60)
                                                            CAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCG                                   <  SRR4302545.2702316/94‑1 (MQ=60)
                                                            CAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCG                                   >  SRR4302545.3492279/1‑94 (MQ=60)
                                                              GATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCC                                 >  SRR4302545.6251341/1‑94 (MQ=60)
                                                                TATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCAT                               >  SRR4302545.6377114/1‑94 (MQ=60)
                                                                TATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCAT                               >  SRR4302545.10310527/1‑94 (MQ=60)
                                                                TATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCAT                               <  SRR4302545.1054913/94‑1 (MQ=60)
                                                                TATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCAT                               <  SRR4302545.1055159/94‑1 (MQ=60)
                                                                 ATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATC                              <  SRR4302545.2859089/94‑1 (MQ=60)
                                                                    GGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGA                           >  SRR4302545.2018471/1‑94 (MQ=60)
                                                                    GGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGA                           >  SRR4302545.2082620/1‑94 (MQ=60)
                                                                    GGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGA                           <  SRR4302545.6751763/94‑1 (MQ=60)
                                                                     GGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGAT                          <  SRR4302545.2059199/94‑1 (MQ=60)
                                                                     GGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGAT                          >  SRR4302545.9472575/1‑94 (MQ=60)
                                                                      GTGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATG                         <  SRR4302545.1961755/94‑1 (MQ=60)
                                                                      GCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATG                         <  SRR4302545.3071888/94‑1 (MQ=60)
                                                                      GCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATG                         >  SRR4302545.1200378/1‑94 (MQ=60)
                                                                      GCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATG                         <  SRR4302545.6473503/94‑1 (MQ=60)
                                                                      GCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATG                         <  SRR4302545.3317174/94‑1 (MQ=60)
                                                                       CGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAATGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGG                        >  SRR4302545.9668659/1‑94 (MQ=60)
                                                                       CGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGG                        >  SRR4302545.10311172/1‑94 (MQ=60)
                                                                         ACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCT                      >  SRR4302545.1097995/1‑94 (MQ=60)
                                                                         ACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCT                      <  SRR4302545.6980701/94‑1 (MQ=60)
                                                                          CAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTT                     >  SRR4302545.7275822/1‑94 (MQ=60)
                                                                          CAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTT                     >  SRR4302545.10327856/1‑94 (MQ=60)
                                                                          CAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTT                     >  SRR4302545.8910839/1‑94 (MQ=60)
                                                                           AGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTA                    <  SRR4302545.10173984/94‑1 (MQ=60)
                                                                           AGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTA                    >  SRR4302545.9059241/1‑94 (MQ=60)
                                                                           AGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTA                    >  SRR4302545.2846168/1‑94 (MQ=60)
                                                                            GCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAA                   <  SRR4302545.3774193/94‑1 (MQ=60)
                                                                            GCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAA                   <  SRR4302545.131450/94‑1 (MQ=60)
                                                                            GCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAA                   >  SRR4302545.4635988/1‑94 (MQ=60)
                                                                              CAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGA                 >  SRR4302545.10829327/1‑94 (MQ=60)
                                                                              CAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGA                 >  SRR4302545.5891037/1‑94 (MQ=60)
                                                                               AGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAA                <  SRR4302545.1168486/94‑1 (MQ=60)
                                                                                GGTTTTCAGCCAGGCGACAGGTGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAAT               >  SRR4302545.8608452/1‑94 (MQ=60)
                                                                                GGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAAT               >  SRR4302545.5184953/1‑94 (MQ=60)
                                                                                GGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAAT               <  SRR4302545.8849542/94‑1 (MQ=60)
                                                                                 GTTTTCATCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATT              <  SRR4302545.10275100/94‑1 (MQ=60)
                                                                                 GTTTTCAGGCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATT              <  SRR4302545.2134882/94‑1 (MQ=60)
                                                                                 GTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATT              <  SRR4302545.2399453/94‑1 (MQ=60)
                                                                                 GTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATT              >  SRR4302545.1239006/1‑94 (MQ=60)
                                                                                  TTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTC             >  SRR4302545.6020207/1‑94 (MQ=60)
                                                                                  TTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTC             <  SRR4302545.9404533/94‑1 (MQ=60)
                                                                                  TTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTC             <  SRR4302545.7944965/94‑1 (MQ=60)
                                                                                   TTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCA            <  SRR4302545.6670249/94‑1 (MQ=60)
                                                                                   TTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCA            <  SRR4302545.4157312/94‑1 (MQ=60)
                                                                                   TTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCA            <  SRR4302545.3854695/94‑1 (MQ=60)
                                                                                    TTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCAT           >  SRR4302545.8915783/1‑94 (MQ=60)
                                                                                    TTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCAT           <  SRR4302545.3628821/94‑1 (MQ=60)
                                                                                     TCAGCCAGGCGACAGGCGATCTTTTGTTTGCCTCAACGCGCAGTTCCGTCGCACCGCCCAGTTGCAGCGCCATCTGATGGCTTAAGAATTGATA          <  SRR4302545.5293312/94‑1 (MQ=55)
                                                                                     TCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATA          <  SRR4302545.7677063/94‑1 (MQ=60)
                                                                                     TCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATA          <  SRR4302545.3919947/94‑1 (MQ=60)
                                                                                     TCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATA          <  SRR4302545.8210957/94‑1 (MQ=60)
                                                                                     TCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATA          <  SRR4302545.8308132/94‑1 (MQ=60)
                                                                                      CAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAG         >  SRR4302545.548631/1‑94 (MQ=60)
                                                                                      CAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAG         >  SRR4302545.6490686/1‑94 (MQ=60)
                                                                                       AGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGT        <  SRR4302545.1987599/94‑1 (MQ=60)
                                                                                         CCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGT      >  SRR4302545.8679276/1‑94 (MQ=60)
                                                                                         CCAGGCGACAGGCGAACTTTTGTTGACCTCAACCCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGT      >  SRR4302545.1397999/1‑94 (MQ=60)
                                                                                         CCAGGCGACAGGCGAACCTTTGTTGACCTCAACGCGCACTTCCGTCAGGCCGCCCAGTTGCTGCGCCATCTCATGGCTTAAGAACTCATAGTGT      >  SRR4302545.1398004/1‑94 (MQ=60)
                                                                                          CAGGCGACAGGCGAACTTTTGTTGACCTCCACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTGATAGTGTT     >  SRR4302545.5782636/1‑94 (MQ=60)
                                                                                          CAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTT     >  SRR4302545.7814557/1‑94 (MQ=60)
                                                                                           AGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTG    >  SRR4302545.8678288/1‑94 (MQ=60)
                                                                                            GGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGC   <  SRR4302545.8421772/94‑1 (MQ=60)
                                                                                            GGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGC   >  SRR4302545.3144379/1‑94 (MQ=60)
                                                                                             GCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCG  <  SRR4302545.2955065/94‑1 (MQ=60)
                                                                                             |                                                                                             
GTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCG  >  NC_000913/3533016‑3533202

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑