Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	882	254.4	12957588	98.0%	12698436	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,533,109	A→G	V133A (GTC→GCC)	envZ ←	protein histidine kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,533,109	0	A	G	100.0%	763.7 / NA	221	V133A (GTC→GCC)	envZ	protein histidine kinase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (128/93); total (128/93)

Only 100 of 243 total aligned reads displayed.

TAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGT  >  NC_000913/3533019‑3533198

 aGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAggcga                                                                                        <  1:5451664/93‑1 (MQ=255)

 aGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAggcga                                                                                        >  2:3826380/1‑93 (MQ=255)

   cGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACa                                                                                      <  2:2650433/93‑1 (MQ=255)

    ggAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAg                                                                                     >  2:936763/1‑93 (MQ=255)

     gtaCAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGTGGCACGCGTACACAGATATAGGGCGACAGCCAGGTTTTCAACCAGGCGACAgg                                                                                    >  2:6333805/3‑93 (MQ=255)

     gAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAgg                                                                                    >  2:2506186/1‑93 (MQ=255)

     gAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAgg                                                                                    >  1:3498036/1‑93 (MQ=255)

     gAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAgg                                                                                    >  2:3354608/1‑93 (MQ=255)

      aaCAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGc                                                                                   <  2:1794397/93‑1 (MQ=255)

             gagagaAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGCCAGCCAGGTTTTCAGACAGGCGACAGGCGAACttt                                                                            >  1:1940938/1‑93 (MQ=255)

             gagagaAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACttt                                                                            <  2:1905312/93‑1 (MQ=255)

             gagagaAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACttt                                                                            <  2:436099/93‑1 (MQ=255)

             gagagaAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACttt                                                                            >  2:5426556/1‑93 (MQ=255)

             gagagaAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACttt                                                                            <  2:2326904/93‑1 (MQ=255)

              agagaAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACtttt                                                                           <  2:4832172/93‑1 (MQ=255)

                    aTCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGAc                                                                     >  1:698567/1‑93 (MQ=255)

                    aTCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGAc                                                                     <  2:3068108/93‑1 (MQ=255)

                       gCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTc                                                                  >  2:1980071/1‑93 (MQ=255)

                       gCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTc                                                                  <  1:4729775/93‑1 (MQ=255)

                       gCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTc                                                                  >  2:3583996/1‑93 (MQ=255)

                       gCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACACGCGAACTTTTGTTGACCTc                                                                  >  1:3301301/1‑93 (MQ=255)

                        cccTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCa                                                                 <  2:4583740/93‑1 (MQ=255)

                         ccTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCaa                                                                >  2:5127257/1‑93 (MQ=255)

                         ccTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCaa                                                                >  2:1366978/1‑93 (MQ=255)

                         ccTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCaa                                                                <  2:5155902/93‑1 (MQ=255)

                         ccTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCaa                                                                <  2:762872/93‑1 (MQ=255)

                         ccTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCaa                                                                >  1:5381135/1‑93 (MQ=255)

                          cTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAAc                                                               <  2:3305190/93‑1 (MQ=255)

                           tgatgaATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAAcg                                                              <  1:123740/93‑1 (MQ=255)

                           tgatgaATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAAcg                                                              >  1:4485501/1‑93 (MQ=255)

                            gatgaATTTCGGTGAGCGGCACGCGTACCCAGATTTTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAAcgc                                                             <  1:3430097/93‑1 (MQ=255)

                               gaATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCa                                                          >  2:1030383/1‑93 (MQ=255)

                                 aTTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACt                                                        >  1:5355438/1‑93 (MQ=255)

                                    tCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTcc                                                     >  2:1298720/1‑93 (MQ=255)

                                      ggTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGt                                                   >  2:5033181/1‑93 (MQ=255)

                                      ggTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGt                                                   >  1:2865874/1‑93 (MQ=255)

                                      ggTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGt                                                   <  1:942105/93‑1 (MQ=255)

                                       gTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTc                                                  <  1:2530957/93‑1 (MQ=255)

                                       gTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTc                                                  >  2:2853638/1‑93 (MQ=255)

                                       gTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTc                                                  >  1:5490412/1‑93 (MQ=255)

                                        tCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCg                                                 >  1:4881599/1‑93 (MQ=255)

                                        tCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGCCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCg                                                 <  2:4014222/93‑1 (MQ=255)

                                           gCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGgc                                              >  1:1594957/1‑93 (MQ=255)

                                             ggCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGgccg                                            >  1:1048674/1‑93 (MQ=255)

                                             ggCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGgccg                                            >  1:4993046/1‑93 (MQ=255)

                                             ggCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGgccg                                            >  1:440550/1‑93 (MQ=255)

                                                aCGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGccc                                         >  2:3703622/1‑93 (MQ=255)

                                                  gcgTACCCAGATATTGGGCGACAGCCAGGTTTTCATCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAg                                       >  1:132956/1‑93 (MQ=255)

                                                  gcgTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAg                                       <  1:2673880/93‑1 (MQ=255)

                                                   cgTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGt                                      <  2:226089/93‑1 (MQ=255)

                                                    gTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGtt                                     >  1:6227849/1‑93 (MQ=255)

                                                    gTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGtt                                     >  1:2329943/1‑93 (MQ=255)

                                                    gTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGtt                                     >  1:2723470/1‑93 (MQ=255)

                                                     tACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTtg                                    >  1:5755715/1‑93 (MQ=255)

                                                     tACCCAGATATTGGGCGACAGCCAGGCTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTtg                                    >  2:4240497/1‑93 (MQ=255)

                                                      aCCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTtgc                                   <  1:5515282/93‑1 (MQ=255)

                                                      aCCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTtgc                                   <  1:337774/93‑1 (MQ=255)

                                                       cccAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTtgct                                  >  1:4532263/1‑93 (MQ=255)

                                                       cccAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTtgct                                  >  1:5228774/1‑93 (MQ=255)

                                                       cccAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTtgct                                  >  1:3073798/1‑93 (MQ=255)

                                                         cAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGGCGCCCAGTtgctgc                                >  2:1149514/1‑93 (MQ=255)

                                                         cAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTtgctgc                                <  2:4109177/93‑1 (MQ=255)

                                                         cAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTtgctgc                                >  2:5892900/1‑93 (MQ=255)

                                                         cAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTtgctgc                                >  1:419908/1‑93 (MQ=255)

                                                         cAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTtgctgc                                >  2:3842303/1‑93 (MQ=255)

                                                         cAGATATTGGGCGACAGCCAGGTGTTCAGCCAGGCGACAGGCGACCTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTtgctgc                                <  1:5308039/93‑1 (MQ=255)

                                                           gATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTgcgc                              >  2:4739714/1‑93 (MQ=255)

                                                            atatTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGcc                             >  1:1750972/1‑93 (MQ=255)

                                                            atatTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGcc                             >  1:3839070/1‑93 (MQ=255)

                                                              atTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCAt                           >  2:1629498/1‑93 (MQ=255)

                                                                 gggCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGGGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTg                        <  2:4276637/93‑1 (MQ=255)

                                                                   gCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGAt                      <  2:4947949/93‑1 (MQ=255)

                                                                    cGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATg                     <  1:4964918/93‑1 (MQ=255)

                                                                    cGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATg                     <  1:5162693/93‑1 (MQ=255)

                                                                     gACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATgg                    >  2:6129507/1‑93 (MQ=255)

                                                                     gACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATgg                    <  1:2171955/93‑1 (MQ=255)

                                                                      aCAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGc                   <  2:3599550/93‑1 (MQ=255)

                                                                          ccAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTaa               <  2:1423437/93‑1 (MQ=255)

                                                                          ccAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTaa               <  2:4475326/93‑1 (MQ=255)

                                                                            aGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGa             >  2:155435/1‑93 (MQ=255)

                                                                            aGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGa             >  2:4846838/1‑93 (MQ=255)

                                                                            aGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGa             <  1:582257/93‑1 (MQ=255)

                                                                             gtttttCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGTCTTAAGaa            <  2:2721532/91‑1 (MQ=255)

                                                                             ggTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGaa            >  1:381201/1‑93 (MQ=255)

                                                                              gTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAAt           <  1:1465359/93‑1 (MQ=255)

                                                                               ttttCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAAtt          >  1:3189622/1‑93 (MQ=255)

                                                                                tttCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTc         >  2:4791257/1‑93 (MQ=255)

                                                                                 ttCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCa        >  1:5437473/1‑93 (MQ=255)

                                                                                 ttCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCa        >  1:5998406/1‑93 (MQ=255)

                                                                                     gCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAgt    <  2:2451988/93‑1 (MQ=255)

                                                                                     gCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAgt    >  1:2782692/1‑93 (MQ=255)

                                                                                     gCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAgt    <  2:5673480/93‑1 (MQ=255)

                                                                                     gCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAgt    <  1:6426986/93‑1 (MQ=255)

                                                                                     gCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAgt    <  1:2228865/93‑1 (MQ=255)

                                                                                      ccAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAgtg   <  2:5561185/93‑1 (MQ=255)

                                                                                       caggcgacaggcgaACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAgtgt  >  2:3197208/1‑93 (MQ=255)

                                                                                       caggcgacaggcgaACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAgtgt  >  1:4103306/1‑93 (MQ=255)

                                                                                       caggcgacaggcgaACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAgtgt  >  2:6442425/1‑93 (MQ=255)

                                                                                       caggcgacaggcgaACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAgtgt  <  1:3934367/93‑1 (MQ=255)

                                                                                       caggcgacaggcgaACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGAGCCATCTGATGGCTTAAGAATTCATAgtgt  >  2:5741587/1‑93 (MQ=255)

TAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGT  >  NC_000913/3533019‑3533198

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCG  >  NC_000913/3533016‑3533202

GTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGG                                                                                               >  SRR4302537.426169/1‑94 (MQ=60)

GTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGG                                                                                               >  SRR4302537.2756554/1‑94 (MQ=60)

GTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGG                                                                                               <  SRR4302537.5270109/94‑1 (MQ=60)

 TATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGC                                                                                              >  SRR4302537.3669150/1‑94 (MQ=60)

  ATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCG                                                                                             <  SRR4302537.2813498/94‑1 (MQ=60)

  ATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCG                                                                                             >  SRR4302537.3662735/1‑94 (MQ=60)

  ATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCG                                                                                             <  SRR4302537.3551883/94‑1 (MQ=60)

    AGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGAC                                                                                           <  SRR4302537.5359957/94‑1 (MQ=60)

    AGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGAC                                                                                           <  SRR4302537.5488849/94‑1 (MQ=60)

      CGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAG                                                                                         >  SRR4302537.2197542/1‑94 (MQ=60)

       GGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGG                                                                                        >  SRR4302537.3522787/1‑94 (MQ=60)

          ACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGA                                                                                     <  SRR4302537.154436/94‑1 (MQ=60)

           CAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAA                                                                                    >  SRR4302537.2727115/1‑94 (MQ=60)

           CAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAA                                                                                    <  SRR4302537.676423/94‑1 (MQ=60)

            AGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAAC                                                                                   >  SRR4302537.2784233/1‑94 (MQ=60)

              CGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTT                                                                                 >  SRR4302537.335475/1‑94 (MQ=60)

               GGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGCCAGCCAGGTTTTCAGACAGGCGACAGGCGAACTTT                                                                                >  SRR4302537.1954433/1‑94 (MQ=60)

                GAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTT                                                                               >  SRR4302537.2737596/1‑94 (MQ=60)

                GAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTT                                                                               <  SRR4302537.1705997/94‑1 (MQ=60)

                GAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTT                                                                               <  SRR4302537.994432/94‑1 (MQ=60)

                 AGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTG                                                                              <  SRR4302537.3288969/94‑1 (MQ=60)

                 AGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTG                                                                              >  SRR4302537.4555727/1‑94 (MQ=60)

                  GAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGT                                                                             <  SRR4302537.5492631/94‑1 (MQ=60)

                   AGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTT                                                                            <  SRR4302537.5214546/94‑1 (MQ=60)

                     AAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGA                                                                          >  SRR4302537.4961795/1‑94 (MQ=60)

                      AATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGAC                                                                         >  SRR4302537.703351/1‑94 (MQ=60)

                       ATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACC                                                                        <  SRR4302537.2594266/94‑1 (MQ=60)

                       ATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACC                                                                        >  SRR4302537.3043460/1‑94 (MQ=60)

                         CGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACACGCGAACTTTTGTTGACCTC                                                                      >  SRR4302537.3324642/1‑94 (MQ=60)

                          GCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCA                                                                     <  SRR4302537.4762459/94‑1 (MQ=60)

                          GCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCA                                                                     >  SRR4302537.4211148/1‑94 (MQ=60)

                          GCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCA                                                                     >  SRR4302537.2881008/1‑94 (MQ=60)

                           CCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAA                                                                    >  SRR4302537.2457011/1‑94 (MQ=60)

                           CCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAA                                                                    >  SRR4302537.5757186/1‑94 (MQ=60)

                           CCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAA                                                                    >  SRR4302537.5827092/1‑94 (MQ=60)

                           CCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAA                                                                    >  SRR4302537.5417933/1‑94 (MQ=60)

                             CTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACG                                                                  >  SRR4302537.4516529/1‑94 (MQ=60)

                             CTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACG                                                                  <  SRR4302537.3889441/94‑1 (MQ=60)

                              TGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGC                                                                 <  SRR4302537.124629/94‑1 (MQ=60)

                              TGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGGTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGC                                                                 >  SRR4302537.4544961/1‑94 (MQ=60)

                               GATGAATTTCGGTGAGCGGCACGCGTACCCAGATTTTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCG                                                                <  SRR4302537.3454372/94‑1 (MQ=60)

                                   AATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACT                                                            >  SRR4302537.5392121/1‑94 (MQ=60)

                                   AATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACT                                                            >  SRR4302537.5107561/1‑94 (MQ=60)

                                    ATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTT                                                           <  SRR4302537.1293354/94‑1 (MQ=60)

                                       TCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCG                                                        <  SRR4302537.4430983/94‑1 (MQ=60)

                                        CGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGT                                                       >  SRR4302537.2885976/1‑94 (MQ=60)

                                         GGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTC                                                      <  SRR4302537.948604/94‑1 (MQ=60)

                                         GGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTC                                                      >  SRR4302537.5527898/1‑94 (MQ=60)

                                          GTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCG                                                     <  SRR4302537.2805271/94‑1 (MQ=60)

                                          GTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCG                                                     <  SRR4302537.2548716/94‑1 (MQ=60)

                                          GTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCG                                                     >  SRR4302537.4951814/1‑94 (MQ=60)

                                          GTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCG                                                     >  SRR4302537.4915321/1‑94 (MQ=60)

                                           TCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGG                                                    >  SRR4302537.5536815/1‑94 (MQ=60)

                                             AGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGC                                                  >  SRR4302537.1606103/1‑94 (MQ=60)

                                               CGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCG                                                >  SRR4302537.5027452/1‑94 (MQ=60)

                                               CGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCG                                                >  SRR4302537.443609/1‑94 (MQ=60)

                                               CGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCG                                                <  SRR4302537.5006932/94‑1 (MQ=60)

                                               CGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCG                                                >  SRR4302537.1055906/1‑94 (MQ=60)

                                                 GCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCTGTCTGGCCGCC                                              >  SRR4302537.3986229/1‑94 (MQ=60)

                                                    CGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCATCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAG                                           >  SRR4302537.133898/1‑94 (MQ=60)

                                                    CGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAG                                           >  SRR4302537.6048300/1‑94 (MQ=60)

                                                    CGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAG                                           >  SRR4302537.1968857/1‑94 (MQ=60)

                                                     GCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGT                                          <  SRR4302537.2692554/94‑1 (MQ=60)

                                                      CGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTT                                         >  SRR4302537.2346374/1‑94 (MQ=60)

                                                      CGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTT                                         >  SRR4302537.6270698/1‑94 (MQ=60)

                                                      CGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTT                                         >  SRR4302537.2742492/1‑94 (MQ=60)

                                                       GTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTG                                        <  SRR4302537.3421353/94‑1 (MQ=60)

                                                       GTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTG                                        >  SRR4302537.5795136/1‑94 (MQ=60)

                                                        TACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGC                                       >  SRR4302537.4786760/1‑94 (MQ=60)

                                                         ACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCT                                      >  SRR4302537.3544217/1‑94 (MQ=60)

                                                         ACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCT                                      >  SRR4302537.4563650/1‑94 (MQ=60)

                                                         ACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCT                                      >  SRR4302537.3095215/1‑94 (MQ=60)

                                                         ACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCT                                      <  SRR4302537.5552941/94‑1 (MQ=60)

                                                         ACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCT                                      <  SRR4302537.340116/94‑1 (MQ=60)

                                                         ACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCT                                      >  SRR4302537.5264733/1‑94 (MQ=60)

                                                          CCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTG                                     >  SRR4302537.506983/1‑94 (MQ=60)

                                                           CCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGC                                    <  SRR4302537.4294548/94‑1 (MQ=60)

                                                           CCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGC                                    >  SRR4302537.5485561/1‑94 (MQ=60)

                                                           CCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGC                                    >  SRR4302537.422819/1‑94 (MQ=60)

                                                            CAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCG                                   <  SRR4302537.5290511/94‑1 (MQ=60)

                                                            CAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCG                                   <  SRR4302537.1823461/94‑1 (MQ=60)

                                                            CAGATATTGGGCGACAGCCAGGTGTTCAGCCAGGCGACAGGCGACCTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCG                                   <  SRR4302537.5344511/94‑1 (MQ=60)

                                                             AGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGC                                  >  SRR4302537.6320742/1‑94 (MQ=60)

                                                              GATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCC                                 >  SRR4302537.3865964/1‑94 (MQ=60)

                                                              GATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCC                                 >  SRR4302537.1763175/1‑94 (MQ=60)

                                                               ATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCA                                >  SRR4302537.1425155/1‑94 (MQ=60)

                                                               ATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCA                                >  SRR4302537.5940926/1‑94 (MQ=60)

                                                                  TTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCT                             <  SRR4302537.4687169/94‑1 (MQ=60)

                                                                  TTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCT                             <  SRR4302537.549404/94‑1 (MQ=60)

                                                                   TGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTG                            >  SRR4302537.6108373/1‑94 (MQ=60)

                                                                   TGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTG                            >  SRR4302537.2288331/1‑94 (MQ=60)

                                                                     GGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGAT                          <  SRR4302537.5445984/94‑1 (MQ=60)

                                                                     GGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGAT                          >  SRR4302537.3412286/1‑94 (MQ=60)

                                                                       CGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGG                        >  SRR4302537.1662363/1‑94 (MQ=60)

                                                                       CGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGG                        <  SRR4302537.4999142/94‑1 (MQ=60)

                                                                       CGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGG                        <  SRR4302537.5198277/94‑1 (MQ=60)

                                                                        GACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGC                       <  SRR4302537.6484195/94‑1 (MQ=60)

                                                                        GACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGC                       <  SRR4302537.2187265/94‑1 (MQ=60)

                                                                           AGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTA                    <  SRR4302537.4447789/94‑1 (MQ=60)

                                                                             CCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAG                  <  SRR4302537.3183502/94‑1 (MQ=60)

                                                                             CCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAG                  <  SRR4302537.981209/94‑1 (MQ=60)

                                                                              CAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGccttcaccccccccttccgccggcccccccggtgctggggccacttgttgctttagaa                 >  SRR4302537.5586554/1‑36 (MQ=60)

                                                                              CAGGTTGTCAGCCAGGCGACAGGCGAACTTTTGGTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGA                 <  SRR4302537.2448426/94‑1 (MQ=60)

                                                                               AGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAA                <  SRR4302537.586244/94‑1 (MQ=60)

                                                                               AGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAA                >  SRR4302537.383844/1‑94 (MQ=60)

                                                                               AGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAA                <  SRR4302537.205708/94‑1 (MQ=60)

                                                                                GGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAAT               <  SRR4302537.384396/94‑1 (MQ=60)

                                                                                 GTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATT              >  SRR4302537.5657040/1‑94 (MQ=60)

                                                                                 GTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATT              <  SRR4302537.1475555/94‑1 (MQ=60)

                                                                                 GTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATT              >  SRR4302537.3212055/1‑94 (MQ=60)

                                                                                 GTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATT              >  SRR4302537.5311331/1‑94 (MQ=60)

                                                                                   TTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCA            >  SRR4302537.5474564/1‑94 (MQ=60)

                                                                                   TTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCA            >  SRR4302537.6039613/1‑94 (MQ=60)

                                                                                    TTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCAT           <  SRR4302537.1432742/94‑1 (MQ=60)

                                                                                      CAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAG         <  SRR4302537.3224459/94‑1 (MQ=60)

                                                                                       AGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGT        >  SRR4302537.2802145/1‑94 (MQ=60)

                                                                                       AGCCAGGCGACAGGCGAACTTTTGTTGACATCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGT        <  SRR4302537.5404812/94‑1 (MQ=60)

                                                                                        GCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTG       <  SRR4302537.2244580/94‑1 (MQ=60)

                                                                                        GCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTG       <  SRR4302537.6470660/94‑1 (MQ=60)

                                                                                         CCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGT      >  SRR4302537.914022/1‑94 (MQ=60)

                                                                                         CCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGT      >  SRR4302537.4131964/1‑94 (MQ=60)

                                                                                         CCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGT      >  SRR4302537.5420592/1‑94 (MQ=60)

                                                                                         CCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGT      >  SRR4302537.1185633/1‑94 (MQ=60)

                                                                                          CAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTT     <  SRR4302537.3976121/94‑1 (MQ=60)

                                                                                          CAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTT     <  SRR4302537.3961862/94‑1 (MQ=60)

                                                                                          CAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTT     <  SRR4302537.3496553/94‑1 (MQ=60)

                                                                                           AGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTG    >  SRR4302537.5813157/1‑94 (MQ=60)

                                                                                            GGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGC   <  SRR4302537.4864145/94‑1 (MQ=60)

                                                                                            GGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGC   <  SRR4302537.3622075/94‑1 (MQ=60)

                                                                                            GGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGC   >  SRR4302537.1431108/1‑94 (MQ=60)

                                                                                             GCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCG  >  SRR4302537.2126986/1‑94 (MQ=60)

GTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCG  >  NC_000913/3533016‑3533202

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 10 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`