Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 647 270.1 21258240 98.4% 20918108 94.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,533,109 A→G V133A (GTC→GCC)  envZ ← protein histidine kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,533,1090AG98.6% 1885.6 / 3.9 556V133A (GTC→GCC) envZprotein histidine kinase
Reads supporting (aligned to +/- strand):  ref base A (1/7);  new base G (243/305);  total (244/312)
Fisher's exact test for biased strand distribution p-value = 8.48e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 597 total aligned reads displayed.

ATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTT  >  NC_000913/3533018‑3533199
                                                                                           |                                                                                          
ataGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAggcg                                                                                          <  1:723536/94‑1 (MQ=255)
 taGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAggcgc                                                                                         >  1:2712758/1‑93 (MQ=255)
   gCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACa                                                                                       <  1:528926/94‑1 (MQ=255)
    cGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAg                                                                                      >  1:8966489/1‑94 (MQ=255)
    cGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAg                                                                                      <  1:7609077/94‑1 (MQ=255)
     tgAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAgg                                                                                     >  1:3254412/2‑94 (MQ=255)
     ggAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAgg                                                                                     >  2:4131540/1‑94 (MQ=255)
     ggAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAgg                                                                                     <  2:2993778/94‑1 (MQ=255)
      gAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGc                                                                                    >  1:2902990/1‑94 (MQ=255)
          aGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAAc                                                                                >  2:2189829/1‑94 (MQ=255)
          aGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAAc                                                                                >  2:4984857/1‑94 (MQ=255)
           gCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACt                                                                               >  2:10508886/1‑94 (MQ=255)
           gCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACt                                                                               <  1:9508014/94‑1 (MQ=255)
            cGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACtt                                                                              <  2:6785788/94‑1 (MQ=255)
            cGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACtt                                                                              <  2:7931405/94‑1 (MQ=255)
             ggAGAGAAATCGCCCTGATGAATTTCGGTTAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACttt                                                                             >  2:6601453/1‑94 (MQ=255)
             ggAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACttt                                                                             >  1:4238678/1‑94 (MQ=255)
               agagaAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTttg                                                                           <  1:3905301/94‑1 (MQ=255)
                gagaAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTttgt                                                                          <  2:5769986/94‑1 (MQ=255)
                  gaAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTttgttg                                                                        <  1:1618665/94‑1 (MQ=255)
                      tCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCt                                                                    <  1:7386707/94‑1 (MQ=255)
                      tCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCt                                                                    <  1:10481028/94‑1 (MQ=255)
                      tCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCt                                                                    <  2:9840864/94‑1 (MQ=255)
                       cGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTc                                                                   >  2:6422143/1‑94 (MQ=255)
                        gCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCa                                                                  <  1:1554349/94‑1 (MQ=255)
                          ccTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGACGAACTTTTGTTGACCTCAAc                                                                >  1:8699317/1‑94 (MQ=255)
                           cTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGTGCGACAGCCAGGTTTTCAGCCAGGGGACAGGCGAACTTTTGTTGACCTCAAcg                                                               >  2:8350649/1‑94 (MQ=255)
                            tgatgaATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCGGGCGAAGGGCCAACTTTTGTTGACTTCAAcgc                                                              >  2:3464057/1‑94 (MQ=255)
                             gatgaATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGCCCTCAAcgcg                                                             <  1:2468402/94‑1 (MQ=255)
                             gatgaATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAAcgcg                                                             <  2:1269114/94‑1 (MQ=255)
                             gatgaATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAAcgcg                                                             <  1:6444981/94‑1 (MQ=255)
                              atgaATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACgcgc                                                            >  2:9641844/1‑94 (MQ=255)
                              atgaATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACgcgc                                                            <  2:8574602/94‑1 (MQ=255)
                              atgaATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACgcgc                                                            >  1:4522940/1‑94 (MQ=255)
                              atgaATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACgcgc                                                            >  2:5661123/1‑94 (MQ=255)
                                gaATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCAc                                                          >  2:8717288/1‑94 (MQ=255)
                                 aaTTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACt                                                         >  2:8829132/1‑94 (MQ=255)
                                 aaTTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACt                                                         <  2:7824964/94‑1 (MQ=255)
                                  aTTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACtt                                                        <  2:8250081/94‑1 (MQ=255)
                                  aTTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACtt                                                        >  2:2730070/1‑94 (MQ=255)
                                   tttCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTc                                                       >  2:176329/1‑94 (MQ=255)
                                   tttCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTc                                                       <  1:6181601/94‑1 (MQ=255)
                                   tttCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTc                                                       <  2:3230566/94‑1 (MQ=255)
                                   tttCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTc                                                       <  1:9288832/94‑1 (MQ=255)
                                     tCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCg                                                     <  1:8209564/94‑1 (MQ=255)
                                     tCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGATTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCg                                                     <  1:5455525/94‑1 (MQ=255)
                                        gTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCg                                                  >  1:7070683/1‑94 (MQ=255)
                                         tCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCgg                                                 <  1:3403292/94‑1 (MQ=255)
                                          cAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCggg                                                >  2:7367481/1‑94 (MQ=255)
                                          cAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTCGACCTCAACGCGCACTTCCGTCggg                                                >  2:7226399/1‑94 (MQ=255)
                                           aGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGgc                                               >  2:8082230/1‑94 (MQ=255)
                                           aGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGgc                                               >  2:2575776/1‑94 (MQ=255)
                                             tggCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGgccg                                             >  1:1067509/2‑94 (MQ=255)
                                                cACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGccc                                          <  2:9113880/94‑1 (MQ=255)
                                                 aCGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGCCAGGCGAACTTTTCTTGACCTCAACGGGCACTTCCGTCGGGccccccg                                         >  2:7066806/1‑93 (MQ=255)
                                                 aCGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCa                                         >  1:5854704/1‑94 (MQ=255)
                                                 aCGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCa                                         <  2:1517420/94‑1 (MQ=255)
                                                 aCGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCa                                         <  2:4302152/94‑1 (MQ=255)
                                                    cgTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGtt                                      <  2:10050167/94‑1 (MQ=255)
                                                    cgTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGtt                                      <  2:7591727/94‑1 (MQ=255)
                                                    cgTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGtt                                      >  2:1172962/1‑94 (MQ=255)
                                                    cgTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGtt                                      <  2:2410651/94‑1 (MQ=255)
                                                     gTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTtg                                     >  1:5674058/1‑94 (MQ=255)
                                                       aCCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTtgct                                   <  2:4389697/94‑1 (MQ=255)
                                                       aCCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTtgct                                   >  2:4720867/1‑94 (MQ=255)
                                                        cccAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTtgctg                                  <  1:10032290/94‑1 (MQ=255)
                                                        cccAGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTtgctg                                  >  1:4813270/1‑94 (MQ=255)
                                                           aGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTgcgc                               >  2:1916044/1‑94 (MQ=255)
                                                           aGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTgcgc                               >  1:137188/1‑94 (MQ=255)
                                                           aGATATTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTgcgc                               >  2:5214456/1‑94 (MQ=255)
                                                            gctatTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGcc                              <  2:1087366/92‑1 (MQ=255)
                                                              tatTGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCAt                            <  1:1032294/94‑1 (MQ=255)
                                                                ttGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCt                          <  2:9755399/94‑1 (MQ=255)
                                                                ttGGGCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCt                          <  2:6003613/94‑1 (MQ=255)
                                                                  gggCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGa                        <  1:6571928/94‑1 (MQ=255)
                                                                   ggCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGAt                       <  1:2009534/94‑1 (MQ=255)
                                                                    gCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATg                      <  2:2099951/94‑1 (MQ=255)
                                                                    gCGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATg                      <  1:6301687/94‑1 (MQ=255)
                                                                     cGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATgg                     <  2:3618366/94‑1 (MQ=255)
                                                                     cGACAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATgg                     <  2:5174348/94‑1 (MQ=255)
                                                                       aCAGCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCt                   <  1:6794172/94‑1 (MQ=255)
                                                                          gCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTaa                <  2:3875568/94‑1 (MQ=255)
                                                                          gCCAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTaa                <  1:3684044/94‑1 (MQ=255)
                                                                           ccAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAg               >  2:3407417/1‑94 (MQ=255)
                                                                           ccAGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAg               <  1:8604063/94‑1 (MQ=255)
                                                                             aGGTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGaa             <  1:1143430/94‑1 (MQ=255)
                                                                              tgTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAAt            <  1:1231885/93‑1 (MQ=255)
                                                                              ggTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAAt            <  2:10192698/94‑1 (MQ=255)
                                                                               gTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAAtt           <  1:2342426/94‑1 (MQ=255)
                                                                               gTTTTCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAAtt           <  1:9695380/94‑1 (MQ=255)
                                                                                 tttCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCa         <  1:10010466/94‑1 (MQ=255)
                                                                                  ttCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCAt        <  1:3542121/94‑1 (MQ=255)
                                                                                   tCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATa       <  1:2611873/94‑1 (MQ=255)
                                                                                   tCAGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATa       <  1:7983904/94‑1 (MQ=255)
                                                                                    cAGCCAGGCGACAGGCGAACTTTTGTTAACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAg      <  1:1887772/94‑1 (MQ=255)
                                                                                     aGCCAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAgt     <  1:3040711/94‑1 (MQ=255)
                                                                                       ccAGGCGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAgtgt   >  1:8435368/1‑94 (MQ=255)
                                                                                        caggcgacaggcgaACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGtt  >  2:6186345/1‑94 (MQ=255)
                                                                                        caggcgacaggcgaACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGtt  <  2:9292219/94‑1 (MQ=255)
                                                                                        caggcgacaggcgaACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGtt  >  1:5143783/1‑94 (MQ=255)
                                                                                           |                                                                                          
ATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTT  >  NC_000913/3533018‑3533199

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 377 total aligned reads displayed.
GCGCTGGCGGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCA‑‑CCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGG  >  NC_000913/3537312‑3537498
                                                                                                |                                                                                             
 CGCTGGCGGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTTGCACGAAATCGTAGGGCAGATAAGGCGTTCA‑‑C                                                                                               <  SRR4302545.9961018/94‑1 (MQ=60)
 CGCTGGCGGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCA‑‑CG                                                                                              <  SRR4302545.1160874/94‑1 (MQ=60)
 CGCTGGCGGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCA‑‑CG                                                                                              >  SRR4302545.747395/1‑94 (MQ=60)
 CGCTGGCGGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCA‑‑CG                                                                                              <  SRR4302545.3822650/94‑1 (MQ=60)
 CGCTGGCGGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCA‑‑CG                                                                                              <  SRR4302545.10886292/94‑1 (MQ=60)
 CGCTGGCGGCGGCAATGGGCAAAAAACGTTAAACGCCCGGACTGGCCTACGGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCA‑‑CG                                                                                              >  SRR4302545.3744426/1‑94 (MQ=60)
   CTGGCGGCGGCAATGGGCAAAAATCGTTAAACGCCCGTACTGGCCTACGGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCA‑‑Cgcc                                                                                            <  SRR4302545.4577472/94‑4 (MQ=60)
   CTGGCGGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCA‑‑Cgcc                                                                                            <  SRR4302545.9192684/94‑4 (MQ=60)
   CTGGCGGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCA‑‑Cgcc                                                                                            >  SRR4302545.1654711/1‑91 (MQ=60)
    TGGCGGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCA‑‑Cgccg                                                                                           >  SRR4302545.412026/1‑90 (MQ=60)
    TGGCGGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCA‑‑Cgccg                                                                                           <  SRR4302545.1712092/94‑5 (MQ=60)
     GGCGGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCA‑‑Cgccgc                                                                                          >  SRR4302545.10164219/1‑89 (MQ=60)
     GGCGGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCA‑‑Cgccgc                                                                                          <  SRR4302545.5720178/94‑6 (MQ=60)
       CGGCGGCAATGGGCAAGAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCAT                                                                                          >  SRR4302545.10786283/1‑94 (MQ=60)
       CGGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCAT                                                                                          <  SRR4302545.2590204/94‑1 (MQ=60)
        GGCGGCAATGGGCAAAAAACGTTAACCGCCCGTACTGGCCTACGGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATC                                                                                         <  SRR4302545.3996895/94‑1 (MQ=60)
        GGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATC                                                                                         >  SRR4302545.1325392/1‑94 (MQ=60)
         GCGGCAATGGCCAAAAAACTTTAAACGCCCGTACTGGCCTACGGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCT                                                                                        <  SRR4302545.30991/94‑1 (MQ=60)
          CGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTG                                                                                       >  SRR4302545.6696793/1‑94 (MQ=60)
          CGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTG                                                                                       <  SRR4302545.1509803/94‑1 (MQ=60)
           GGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGG                                                                                      >  SRR4302545.7794973/1‑94 (MQ=60)
           GGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGG                                                                                      <  SRR4302545.2334529/94‑1 (MQ=60)
            GCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGC                                                                                     <  SRR4302545.7146595/94‑1 (MQ=60)
            GCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGC                                                                                     >  SRR4302545.3919864/1‑94 (MQ=60)
            GCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGC                                                                                     >  SRR4302545.9358496/1‑94 (MQ=60)
            GCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGC                                                                                     >  SRR4302545.1393026/1‑94 (MQ=60)
             CAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCA                                                                                    >  SRR4302545.2821096/1‑94 (MQ=60)
             CAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCA                                                                                    <  SRR4302545.5080057/94‑1 (MQ=60)
             CAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCA                                                                                    >  SRR4302545.10825612/1‑94 (MQ=60)
             CAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCA                                                                                    >  SRR4302545.10539316/1‑94 (MQ=60)
             CAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCAGCATCTGGCA                                                                                    >  SRR4302545.3526166/1‑94 (MQ=60)
              AATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAA                                                                                   <  SRR4302545.3893899/94‑1 (MQ=60)
              AATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAA                                                                                   >  SRR4302545.3655412/1‑94 (MQ=60)
               ATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAAC                                                                                  >  SRR4302545.10688530/1‑94 (MQ=60)
                TGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACG                                                                                 >  SRR4302545.3965192/1‑94 (MQ=60)
                  GGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAA                                                                               >  SRR4302545.4345668/1‑94 (MQ=60)
                  GCCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAA                                                                               <  SRR4302545.2709040/94‑1 (MQ=60)
                   GCAAAAAACTTTAACCGCCCGTACTGGCCTAGGGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAAC                                                                              <  SRR4302545.9603807/94‑1 (MQ=60)
                   GCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAAC                                                                              >  SRR4302545.6953983/1‑94 (MQ=60)
                    CAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACG                                                                             >  SRR4302545.6071345/1‑94 (MQ=60)
                    CAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACG                                                                             >  SRR4302545.2495497/1‑94 (MQ=60)
                    CAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACG                                                                             >  SRR4302545.7932592/1‑94 (MQ=60)
                     AAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGC                                                                            <  SRR4302545.8433070/94‑1 (MQ=60)
                     AAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGC                                                                            >  SRR4302545.4819315/1‑94 (MQ=60)
                      AAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCC                                                                           >  SRR4302545.10193803/1‑94 (MQ=60)
                       AAAACGTTAAGCGCCCGTACTGGCCTACGGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCT                                                                          >  SRR4302545.3483402/1‑94 (MQ=60)
                       AAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCT                                                                          >  SRR4302545.3751367/1‑94 (MQ=60)
                       AAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCT                                                                          <  SRR4302545.5491768/94‑1 (MQ=60)
                        AAACGTTAAACGCCCGTACTGGCCTACGGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTT                                                                         >  SRR4302545.4070866/1‑94 (MQ=60)
                        AAACGTTAAACGCCCGTACTGGCCTACGGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTT                                                                         >  SRR4302545.1119848/1‑94 (MQ=60)
                        AAACGTTAAACGCCCGTACTGGCCTACGGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTT                                                                         >  SRR4302545.826052/1‑94 (MQ=60)
                         ACCGTTAAACGCCCGTACTGGCCTACGGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTG                                                                        <  SRR4302545.1819546/94‑1 (MQ=60)
                         AACGTTAAACGCCCGTACTGGCCTACGGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCTTCTGGCAACGAATGCCTTG                                                                        >  SRR4302545.9649285/1‑94 (MQ=60)
                         AACGTTAAACGCCCGTACTGGCCTACGGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTG                                                                        <  SRR4302545.8500624/94‑1 (MQ=60)
                          ACGTTAAACGCCCGTACTGGCCTACGGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGC                                                                       >  SRR4302545.10383392/1‑94 (MQ=60)
                          ACGTTAAACGCCCGTACTGGCCTACGGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGC                                                                       <  SRR4302545.196874/94‑1 (MQ=60)
                           CGTTAACCGCCCGTACTGGCCTACGGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCC                                                                      <  SRR4302545.9085996/94‑1 (MQ=60)
                           CGTTAAACGCCCGTACTGGCCTACGGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCC                                                                      <  SRR4302545.7168881/94‑1 (MQ=60)
                           CGTTAAACGCCCGTACTGGCCTACGGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCC                                                                      >  SRR4302545.6561896/1‑94 (MQ=60)
                            GTTAAACGCCCGTACTGGCCTACGGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCT                                                                     >  SRR4302545.9891300/1‑94 (MQ=60)
                            GTTAAACGCCCGTACTGGCCTACGGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCT                                                                     >  SRR4302545.8724733/1‑94 (MQ=60)
                             TTAAACGCCCGTACTGGCCTACGGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTG                                                                    >  SRR4302545.8543575/1‑94 (MQ=60)
                             TAAAACGCCCCTCCTGGCCTACGGTTCAAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTG                                                                    <  SRR4302545.7728824/94‑1 (MQ=60)
                              TAAACGCCCGTACTGGCCTACGGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGA                                                                   >  SRR4302545.8557932/1‑94 (MQ=60)
                               AAACGCCCGTACTGGCCTACGGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGAC                                                                  <  SRR4302545.10572012/94‑1 (MQ=60)
                               AAACGCCCGTACTGGCCTACGGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGAC                                                                  >  SRR4302545.5555021/1‑94 (MQ=60)
                               AAACGCCCGTACTGGCCTACGGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGAC                                                                  >  SRR4302545.7506217/1‑94 (MQ=60)
                               AAACGCCCGTACTGGCCTACGGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGAC                                                                  >  SRR4302545.7914712/1‑94 (MQ=60)
                               AAACGCCCGTACTGGCCTACGGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGAC                                                                  >  SRR4302545.5555019/1‑94 (MQ=60)
                                AACGCCCGTACTGGCCTACGGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACA                                                                 <  SRR4302545.6307113/94‑1 (MQ=60)
                                 ACGCCCGTACTGGCCTACGGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACAT                                                                >  SRR4302545.9420292/1‑94 (MQ=60)
                                 ACGCCCGTACTGGCCTACGGTTCGAA‑TTTGCACGAAATCGTAGGGAAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACAT                                                                <  SRR4302545.8475885/94‑1 (MQ=60)
                                  CGCCCGTACTGGCCTACGTTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATA                                                               <  SRR4302545.4388031/94‑1 (MQ=60)
                                  CGCCCGTACTGGCCTACGGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATA                                                               <  SRR4302545.4237590/94‑1 (MQ=60)
                                  CGCCCGTACTGGCCTACGGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATA                                                               >  SRR4302545.6904285/1‑94 (MQ=60)
                                  CGCCCGTACTGGCCTACGGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATA                                                               >  SRR4302545.2401445/1‑94 (MQ=60)
                                  CGCCCGTACTGGCCTACGGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATA                                                               <  SRR4302545.1073168/94‑1 (MQ=60)
                                   GCCCGTACTGGCCTGCGGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAA                                                              <  SRR4302545.570086/94‑1 (MQ=60)
                                   GCCCGTACTGGCCTACGGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAA                                                              >  SRR4302545.4937753/1‑94 (MQ=60)
                                   GCCCGTACTGGCCTACGGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAA                                                              >  SRR4302545.10760310/1‑94 (MQ=60)
                                   GCCCGTACTGGCCTACGGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAA                                                              >  SRR4302545.9238028/1‑94 (MQ=60)
                                   GCCCGTACTGGCCTACGGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAAAGAACGCCTTGCCTGACATAA                                                              >  SRR4302545.9724298/1‑94 (MQ=60)
                                    CCCGTACTGGCCTACGGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATCAA                                                             >  SRR4302545.4619292/1‑94 (MQ=60)
                                    CCCGTACTGGCCTACGGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAA                                                             >  SRR4302545.10451239/1‑94 (MQ=60)
                                    CCCGTACTGGCCTACGGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAA                                                             >  SRR4302545.5200497/1‑94 (MQ=60)
                                    CCCGTACTGGCCTACGGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAA                                                             >  SRR4302545.9346570/1‑94 (MQ=60)
                                    CCCGTACTGGCCTACGGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAA                                                             <  SRR4302545.1101045/94‑1 (MQ=60)
                                     CCGTACTGGCCTACGGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAA                                                            <  SRR4302545.5869475/94‑1 (MQ=60)
                                     CCGTACTGGCCTACGGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAA                                                            >  SRR4302545.1571321/1‑94 (MQ=60)
                                     CCGTACTGGCCTACGGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAA                                                            >  SRR4302545.608504/1‑94 (MQ=60)
                                     CCGTACTGGCCTACGGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAA                                                            >  SRR4302545.6737810/1‑94 (MQ=60)
                                     CCGTACTGGCCTACGGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAA                                                            >  SRR4302545.10555377/1‑94 (MQ=60)
                                      CGTACTGGCCTACGGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAG                                                           >  SRR4302545.8929241/1‑94 (MQ=60)
                                       GTACTGGCCTACGGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCCACGAACGCCTTGCCTGACATAAAAGT                                                          >  SRR4302545.6587924/1‑94 (MQ=60)
                                       GTACTGGCCTACGGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGT                                                          >  SRR4302545.10648107/1‑94 (MQ=60)
                                       GTACTGGCCTACGGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGT                                                          >  SRR4302545.8347933/1‑94 (MQ=60)
                                        TACTGGCCTACGGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTG                                                         <  SRR4302545.6157811/94‑1 (MQ=60)
                                        TACTGGCCTACGGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTG                                                         >  SRR4302545.326323/1‑94 (MQ=60)
                                        TACTGGCCTACGGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTG                                                         <  SRR4302545.7623711/94‑1 (MQ=60)
                                         ACTGGCCTACGGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGC                                                        >  SRR4302545.2760152/1‑94 (MQ=60)
                                         ACTGGCCTACGGTACGAA‑TTTGCACGAAATCGGAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGC                                                        >  SRR4302545.10003598/1‑94 (MQ=60)
                                          CTGGCCTACGGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACagatcagagatcggaagagcgtc                                                       >  SRR4302545.4694997/1‑71 (MQ=60)
                                          CTGGCCTACGGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCC                                                       >  SRR4302545.7109220/1‑94 (MQ=60)
                                            GGCCTACGGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGG                                                     >  SRR4302545.1747237/1‑94 (MQ=60)
                                            GGCCTACGGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGG                                                     <  SRR4302545.1732367/94‑1 (MQ=60)
                                            GGCCTACGGTGCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGG                                                     >  SRR4302545.7239949/1‑94 (MQ=60)
                                              CCTACGGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAG                                                   <  SRR4302545.4652818/94‑1 (MQ=60)
                                              CCTACGGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAG                                                   >  SRR4302545.3727972/1‑94 (MQ=60)
                                              CCTACGGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAG                                                   <  SRR4302545.9860002/94‑1 (MQ=60)
                                               CTACGGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGA                                                  >  SRR4302545.7197486/1‑94 (MQ=60)
                                               CTACGGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGA                                                  >  SRR4302545.6893608/1‑94 (MQ=60)
                                               CTACGGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGA                                                  >  SRR4302545.8156761/1‑94 (MQ=60)
                                               CTACGGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCA‑‑Cagatcagagatcggaagagcgtcgtgtagggaaagagtgtagatctc                                                >  SRR4302545.2779417/1‑47 (MQ=60)
                                                TACGGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAA                                                 >  SRR4302545.6795197/1‑94 (MQ=60)
                                                TACGGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAA                                                 >  SRR4302545.8528858/1‑94 (MQ=60)
                                                TACGGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTCCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAA                                                 <  SRR4302545.4358501/94‑1 (MQ=60)
                                                 ACGGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAGGGCTTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAAT                                                <  SRR4302545.464803/94‑1 (MQ=60)
                                                 ACGGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAAT                                                <  SRR4302545.8769090/94‑1 (MQ=60)
                                                  TGGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATA                                               >  SRR4302545.3327136/1‑94 (MQ=60)
                                                  CGGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATA                                               <  SRR4302545.5830983/94‑1 (MQ=60)
                                                  CGGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATA                                               <  SRR4302545.2663956/94‑1 (MQ=60)
                                                  CGGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATA                                               >  SRR4302545.4849952/1‑94 (MQ=60)
                                                   GGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGGGAATAT                                              >  SRR4302545.2300368/1‑94 (MQ=60)
                                                   GGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATAT                                              <  SRR4302545.2804677/94‑1 (MQ=60)
                                                   GGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATAT                                              >  SRR4302545.10884839/1‑94 (MQ=60)
                                                   GGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATAT                                              >  SRR4302545.345509/1‑94 (MQ=60)
                                                    GTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATC                                             >  SRR4302545.10901215/1‑94 (MQ=60)
                                                    GTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATC                                             >  SRR4302545.3551166/1‑94 (MQ=60)
                                                     TTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCT                                            >  SRR4302545.274325/1‑94 (MQ=60)
                                                      TCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTC                                           >  SRR4302545.3868880/1‑94 (MQ=60)
                                                      TCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTC                                           >  SRR4302545.74658/1‑94 (MQ=60)
                                                      TCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTC                                           >  SRR4302545.7666316/1‑94 (MQ=60)
                                                       CGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGgatattttc                                          >  SRR4302545.5906732/1‑85 (MQ=60)
                                                       CGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCC                                          <  SRR4302545.4656176/94‑1 (MQ=60)
                                                       CGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCC                                          <  SRR4302545.5918755/94‑1 (MQ=60)
                                                        GAA‑TTTTCCCGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCG                                         <  SRR4302545.3225839/94‑1 (MQ=60)
                                                        GAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCG                                         <  SRR4302545.6453745/94‑1 (MQ=60)
                                                        GAA‑TTTGCACGAAAACGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCG                                         <  SRR4302545.2961505/94‑1 (MQ=60)
                                                         AA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGG                                        <  SRR4302545.7345668/94‑1 (MQ=60)
                                                         AA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGG                                        >  SRR4302545.7309036/1‑94 (MQ=60)
                                                          A‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGC                                       >  SRR4302545.8600588/1‑94 (MQ=60)
                                                          A‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGC                                       >  SRR4302545.8093466/1‑94 (MQ=60)
                                                          A‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGC                                       >  SRR4302545.5175606/1‑94 (MQ=60)
                                                          A‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGA                                       >  SRR4302545.4501692/1‑94 (MQ=60)
                                                            TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAGAGTGCCGGAGAATATCTCCGGCA                                      >  SRR4302545.5961769/1‑94 (MQ=60)
                                                            TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCA                                      >  SRR4302545.5021961/1‑94 (MQ=60)
                                                             TTGCACGAAATCGTAGGGCAGATAAGGGGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGCAGAATATCTCCTGCAT                                     >  SRR4302545.6436165/1‑94 (MQ=60)
                                                             TTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCAT                                     <  SRR4302545.1011085/94‑1 (MQ=60)
                                                              TGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATT                                    >  SRR4302545.4453712/1‑94 (MQ=60)
                                                               GCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTT                                   <  SRR4302545.3196240/94‑1 (MQ=60)
                                                                CACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTT                                  >  SRR4302545.3609508/1‑94 (MQ=60)
                                                                 ACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTT                                 <  SRR4302545.6674335/94‑1 (MQ=60)
                                                                 ACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTT                                 <  SRR4302545.4501797/94‑1 (MQ=60)
                                                                 ACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTT                                 >  SRR4302545.4516028/1‑94 (MQ=60)
                                                                  CGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTT                                >  SRR4302545.2683661/1‑94 (MQ=60)
                                                                  CGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTA                                >  SRR4302545.1293715/1‑94 (MQ=60)
                                                                   GAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTAT                               <  SRR4302545.2292404/94‑1 (MQ=60)
                                                                     AATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTC                             <  SRR4302545.10039437/94‑1 (MQ=60)
                                                                      ATGGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCC                            <  SRR4302545.5895861/94‑1 (MQ=60)
                                                                      ATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCC                            <  SRR4302545.9293867/94‑1 (MQ=60)
                                                                       TCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCA                           <  SRR4302545.1940992/94‑1 (MQ=60)
                                                                       TCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCA                           >  SRR4302545.10505976/1‑94 (MQ=60)
                                                                       ACGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCA                           <  SRR4302545.348669/94‑1 (MQ=60)
                                                                        CGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCTC                          >  SRR4302545.9174384/1‑94 (MQ=60)
                                                                        CGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCAC                          >  SRR4302545.9174385/1‑94 (MQ=60)
                                                                        CGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCAC                          >  SRR4302545.675265/1‑94 (MQ=60)
                                                                         GTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACA                         <  SRR4302545.9012076/94‑1 (MQ=60)
                                                                         GTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACA                         >  SRR4302545.6357642/1‑94 (MQ=60)
                                                                         GTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACA                         <  SRR4302545.5322370/94‑1 (MQ=60)
                                                                          gggggcaaataggggttccacgCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAG                        <  SRR4302545.7692988/72‑1 (MQ=60)
                                                                          TGGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAG                        <  SRR4302545.4187793/94‑1 (MQ=60)
                                                                          TAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAG                        <  SRR4302545.5622653/94‑1 (MQ=60)
                                                                          TAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAG                        >  SRR4302545.1584741/1‑94 (MQ=60)
                                                                           AGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGC                       >  SRR4302545.4868375/1‑94 (MQ=60)
                                                                              GCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAA                    <  SRR4302545.10085490/94‑1 (MQ=60)
                                                                                AGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAAC                  >  SRR4302545.4810557/1‑94 (MQ=60)
                                                                                 GATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACT                 <  SRR4302545.10870028/94‑1 (MQ=60)
                                                                                 GATAAGGCGTCCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACT                 <  SRR4302545.2205649/94‑1 (MQ=60)
                                                                                  ATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCAACAGCCAAACTC                >  SRR4302545.3436440/1‑94 (MQ=60)
                                                                                  ATAAGGCGTCCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTC                <  SRR4302545.146701/94‑1 (MQ=60)
                                                                                     agacgtccacgCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATA             <  SRR4302545.9701181/83‑1 (MQ=60)
                                                                                     AGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATA             >  SRR4302545.2956926/1‑94 (MQ=60)
                                                                                         tacCA‑‑CCGCATCAGGCACCGAACGCATAACCTGACATAAAAGTACCGGAGAAAAACTCCGGCATTTTTATTCCACAGCCAAACTCAAAATATTT       <  SRR4302545.9661995/91‑1 (MQ=43)
                                                                                         gtcaacgCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATAT         <  SRR4302545.2138842/87‑1 (MQ=60)
                                                                                         GTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATAT         >  SRR4302545.3304751/1‑94 (MQ=60)
                                                                                         GTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATAT         <  SRR4302545.3921813/94‑1 (MQ=60)
                                                                                          TTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGGATATTTCCGGCATTTTTATTTCACAGCCCAACTCATAATATA        >  SRR4302545.9757442/1‑94 (MQ=60)
                                                                                           tcacgCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATAT       <  SRR4302545.2001926/89‑1 (MQ=60)
                                                                                           tcacgCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATAT       <  SRR4302545.4080412/89‑1 (MQ=60)
                                                                                            cacgCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATT      <  SRR4302545.5116981/90‑1 (MQ=60)
                                                                                            CG‑‑CCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCC    <  SRR4302545.4293164/94‑1 (MQ=60)
                                                                                            CG‑‑CCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCC    >  SRR4302545.2880425/1‑94 (MQ=60)
                                                                                            CG‑‑CCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCC    <  SRR4302545.5364832/94‑1 (MQ=60)
                                                                                             acgCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTTCCCAGCCAAACTCATAATATATTC     >  SRR4302545.7901075/4‑94 (MQ=60)
                                                                                             acgCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTC     <  SRR4302545.1264070/91‑1 (MQ=60)
                                                                                             acgCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTC     >  SRR4302545.2377916/4‑94 (MQ=60)
                                                                                             G‑‑CCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCG   <  SRR4302545.6096697/94‑1 (MQ=60)
                                                                                             G‑‑CCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCG   <  SRR4302545.8367241/94‑1 (MQ=60)
                                                                                             G‑‑CCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCG   >  SRR4302545.5384998/1‑94 (MQ=60)
                                                                                                CCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGG  <  SRR4302545.9944529/94‑1 (MQ=60)
                                                                                                |                                                                                             
GCGCTGGCGGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAA‑TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCA‑‑CCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGG  >  NC_000913/3537312‑3537498

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑