Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,537,405 +GC intergenic (+61/‑394) b3407 → / → feoA hypothetical protein/ferrous iron transport protein A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,537,4041.C99.6% 900.4 / ‑4.0 261intergenic (+60/‑395)b3407/feoAhypothetical protein/ferrous iron transport protein A
Reads supporting (aligned to +/- strand):  ref base . (1/0);  new base C (151/109);  total (152/109)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01
*NC_0009133,537,4042.G99.6% 905.8 / ‑4.0 262intergenic (+60/‑395)b3407/feoAhypothetical protein/ferrous iron transport protein A
Reads supporting (aligned to +/- strand):  ref base . (1/0);  new base G (151/110);  total (152/110)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

Only 100 of 277 total aligned reads displayed.

GCGCTGGCGGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGT‑‑TCA‑‑CCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATA  >  NC_000913/3537312‑3537487
                                                                                               ||                                                                                   
gcgcTGGCGGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGT‑‑TCa                                                                                       <  2:3045378/93‑1 (MQ=255)
    tGGCGGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGT‑‑TCA‑‑cgcc                                                                                 >  1:5829854/1‑90 (MQ=255)
     ggcggcggcAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGT‑‑TCA‑‑cgccg                                                                                >  2:2412739/1‑89 (MQ=255)
           ggcAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGT‑‑TCACGCCGCATCTg                                                                            >  2:4993203/1‑93 (MQ=38)
            gcAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGT‑‑TCACGCCGCATCTgg                                                                           <  2:1105513/93‑1 (MQ=38)
             cAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGT‑‑TCACGCCGCATCTGGc                                                                          >  2:730477/1‑93 (MQ=37)
              aaTGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGT‑‑TCACGCCGCATCTGGCa                                                                         >  1:780861/1‑93 (MQ=37)
              aaTGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGT‑‑TCACGCCGCATCTGGCa                                                                         >  2:2641870/1‑93 (MQ=37)
              aaTGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGT‑‑TCACGCCGCATCTGGCa                                                                         >  1:3796234/1‑93 (MQ=37)
               aTGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGT‑‑TCACGCCGCATCTGGCaa                                                                        >  2:677506/1‑93 (MQ=37)
                tGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGT‑‑TCACGCCGCATCTGGCaac                                                                       >  2:1515225/1‑93 (MQ=37)
                tGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGT‑‑TCACGCCGCATCTGGCaac                                                                       <  2:4029543/93‑1 (MQ=38)
                tGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGT‑‑TCACGCCGCATCTGGCaac                                                                       >  1:1593019/1‑93 (MQ=37)
                  ggCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGT‑‑TCACGCCGCATCTGGCaacga                                                                     >  2:3238721/1‑93 (MQ=37)
                   gCAAAAAACTTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGTCAGATAAGGCGT‑‑TCACGCCGCATCTGGCaacgaa                                                                    <  2:4832411/93‑1 (MQ=25)
                   gCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGT‑‑TCACGCCGCATCTGGCaacgaa                                                                    <  1:2537638/93‑1 (MQ=37)
                   gCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGT‑‑TCACGCCGCATCTGGCaacgaa                                                                    <  1:2698850/93‑1 (MQ=37)
                   gCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGT‑‑TCACGCCGCATCTGGCaacgaa                                                                    <  1:5163042/93‑1 (MQ=37)
                       aaaaCGTTAAACGCCCGTAGTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGT‑‑TCACGCCGCATCTGGCAACGAACGcc                                                                <  1:1369079/93‑1 (MQ=37)
                       aaaaCGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGT‑‑TCACGCCGCATCTGGCAACGAACGcc                                                                >  2:5502885/1‑93 (MQ=37)
                       aaaaCGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGT‑‑TCACGCCGCATCTGGCAACGAACGcc                                                                <  2:3928949/93‑1 (MQ=37)
                       aaaaCGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGT‑‑TCACGCCGCATCTGGCAACGAACGcc                                                                <  1:2026918/93‑1 (MQ=37)
                        aaaCGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGT‑‑TCACGCCGCATCTGGCAACGAACGCCt                                                               <  1:645428/93‑1 (MQ=37)
                          aCGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGT‑‑TCACGCCGCATCTGGCAACGAACGCCTTg                                                             >  1:3540025/1‑93 (MQ=37)
                          aCGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGT‑‑TCACGCCGCATCTGGCAACGAACGCCTTg                                                             >  1:2123050/1‑93 (MQ=37)
                          aCGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGT‑‑TCACGCCGCATCTGGCAACGAACGCCTTg                                                             >  2:1784270/1‑93 (MQ=37)
                           cGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGT‑‑TCACGCCGCATCTGGCAACGAACGCCTTGc                                                            >  2:5195863/1‑93 (MQ=37)
                           cGTTAAACGCCCGTACTGGCCTACGGTTCGAATTGGCACGAAATCGTAGGGCAGATAAGGCGT‑‑TCACGCCGCATCTGGCAACGAACGCCTTGc                                                            <  2:6027395/93‑1 (MQ=37)
                            gTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGT‑‑TCACGCCGCATCTGGCAACGAACGCCTTGcc                                                           <  2:4604987/93‑1 (MQ=37)
                            gTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGT‑‑TCACGCCGCATCTGGCAACGAACGCCTTGcc                                                           <  1:5780736/93‑1 (MQ=37)
                            gTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGT‑‑TCACGCCGCATCTGGCAACGAACGCCTTGcc                                                           >  1:5254242/1‑93 (MQ=37)
                             ttAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGT‑‑TCACGCCGCATCTGGCAACGAACGCCTTGCCt                                                          >  2:1016079/1‑93 (MQ=37)
                             ttAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGT‑‑TCACGCCGCATCTGGCAACGAACGCCTTGCCt                                                          <  2:2683615/93‑1 (MQ=37)
                               aaaCGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGT‑‑TCACGCCGCATCTGGCAACGAACGCCTTGCCTGa                                                        >  2:3658959/1‑93 (MQ=38)
                               aaaCGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGT‑‑TCACGCCGCATCTGGCAACGAACGCCTTGCCTGa                                                        <  2:4377898/93‑1 (MQ=37)
                                    cccGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGT‑‑TCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATaa                                                   >  2:2846295/1‑93 (MQ=38)
                                    cccGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGT‑‑TCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATaa                                                   <  1:597339/93‑1 (MQ=37)
                                      cGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGT‑‑TCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATaaaa                                                 >  2:4441022/1‑93 (MQ=38)
                                       gTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGT‑‑TCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAg                                                >  2:2302632/1‑93 (MQ=38)
                                       gTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGT‑‑TCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAg                                                >  2:1346596/1‑93 (MQ=38)
                                        tccTGGCCGGCGGTTCAAATTCGCACGAAATCGTAGGCCAGATGAGGCGTCCGCG‑‑CCGCATCGGGCAACGAACGCCTTGCCTGACATAAAAGt                                               <  2:4102034/91‑1 (MQ=19)
                                        tACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGT‑‑TCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGt                                               >  2:5379424/1‑93 (MQ=38)
                                        tACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGT‑‑TCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGt                                               <  1:3159249/93‑1 (MQ=37)
                                        tACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGT‑‑TCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGt                                               >  1:4024098/1‑93 (MQ=38)
                                         tcTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGT‑‑TCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTg                                              <  1:1436049/92‑1 (MQ=37)
                                         aCTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGT‑‑TCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTg                                              <  2:46565/93‑1 (MQ=37)
                                          cTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGT‑‑TCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGc                                             >  1:3239106/1‑93 (MQ=38)
                                           tGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGT‑‑TCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGcc                                            >  1:6202927/1‑93 (MQ=37)
                                           tGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGT‑‑TCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGcc                                            <  2:6138790/93‑1 (MQ=37)
                                           tGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGT‑‑TCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGcc                                            >  1:2104625/1‑93 (MQ=38)
                                            ggCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGT‑‑TCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCg                                           >  2:3777736/1‑93 (MQ=38)
                                            ggCCTACGCTTCGAATTTGCACAAAATCGTAGGGCAGATAAGGCGT‑‑TCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCg                                           <  1:174283/93‑1 (MQ=37)
                                              ccTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGT‑‑TCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGTCGga                                         >  1:1195023/1‑93 (MQ=37)
                                               cTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGT‑‑TCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGgag                                        >  2:4149983/1‑93 (MQ=38)
                                                tACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGT‑‑TCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGgaga                                       >  1:1956642/1‑93 (MQ=37)
                                                tACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGT‑‑TCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGgaga                                       <  2:5898632/93‑1 (MQ=37)
                                                  cGGTTCGATTTTGCACGAAATCGTAGGGCAGATAAGGCGT‑‑TCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAat                                     <  2:582181/93‑1 (MQ=37)
                                                  cGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGT‑‑TCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAat                                     <  1:2226830/93‑1 (MQ=37)
                                                  cGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGT‑‑TCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAat                                     <  2:2926116/93‑1 (MQ=37)
                                                   ggTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGT‑‑TCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAata                                    >  2:6407961/1‑93 (MQ=37)
                                                   ggTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGT‑‑TCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAata                                    >  2:4215123/1‑93 (MQ=38)
                                                   ggTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGT‑‑TCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAata                                    >  1:2671252/1‑93 (MQ=38)
                                                    gTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGT‑‑TCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAatat                                   >  2:2199534/1‑93 (MQ=38)
                                                    gTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGT‑‑TCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAatat                                   <  2:5253189/93‑1 (MQ=37)
                                                    gTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGT‑‑TCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAatat                                   >  1:4928635/1‑93 (MQ=38)
                                                    gTTCGAATTTGCACAAAATCGTAGGGCAGATAAGGCGT‑‑TCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAatat                                   <  2:5179018/93‑1 (MQ=37)
                                                     ttCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGT‑‑TCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATAtc                                  >  2:5080790/1‑93 (MQ=38)
                                                      tCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGT‑‑TCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATAtct                                 <  2:2808365/93‑1 (MQ=37)
                                                      tCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGT‑‑TCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATAtct                                 <  1:4296317/93‑1 (MQ=37)
                                                         attttGCACAAAACCGTAGGGCAGATAAGGCGT‑‑TCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCg                              <  2:4492629/91‑1 (MQ=37)
                                                           tttGCACGAAATCGTAGGGCAGATAAGGCGT‑‑TCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGc                            >  2:5779919/1‑93 (MQ=38)
                                                            ttGCACGAAATCGTAGGGGAGATAAGGCGT‑‑TCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCa                           <  1:5052365/93‑1 (MQ=38)
                                                            ttGCACGAAATCGTAGGGCAGATAAGGCGT‑‑TCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCa                           >  2:5983171/1‑93 (MQ=38)
                                                            ttGCACGAAATCGTAGGGCAGATAAGGCGT‑‑TCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCa                           >  2:2497713/1‑93 (MQ=38)
                                                               cACGAAATCGTAGGGCAGATAAGGCGT‑‑TCACGCCGCATCTGGCAACTAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCAttt                        <  2:4899004/93‑1 (MQ=37)
                                                                aCGAAATCGTAGGGCAGATAAGGCGT‑‑TCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCAtttt                       >  2:2066437/1‑93 (MQ=38)
                                                                aCGAAATCGTAGGGCAGATAAGGCGT‑‑TCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCAtttt                       <  1:4161273/93‑1 (MQ=38)
                                                                  gAAATCGTAGGGCAGATAAGGCGT‑‑TCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTa                     <  1:3745088/93‑1 (MQ=38)
                                                                   aaaTCGTAGGGCAGATAAGGCGT‑‑TCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTAt                    >  1:2646131/1‑93 (MQ=38)
                                                                    aaTCGTAGGGCAGATAAGGCGT‑‑TCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCAtttttttt                   >  1:2346963/1‑93 (MQ=37)
                                                                    aaTCGTAGGGCAGATAAGGCGT‑‑TCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTAtt                   <  1:4331553/93‑1 (MQ=38)
                                                                     aTCGTAGGGCAGATAAGGCGT‑‑TCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTc                  >  1:515571/1‑93 (MQ=38)
                                                                       cGTAGGGCAGATAAGGCGT‑‑TCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGGATATCTCCTGCATTTTTATTCca                >  1:5102417/1‑93 (MQ=37)
                                                                       cGTAGGGCAGATAAGGCGT‑‑TCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCca                <  1:4593887/93‑1 (MQ=38)
                                                                         tAGGGCAGATAAGGCGT‑‑TCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGGCGGAGAATATCTCCGGCATTTTTATTCcaca              <  2:4178208/93‑1 (MQ=38)
                                                                         tAGGGCAGATAAGGCGT‑‑TCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCcaca              <  1:104007/93‑1 (MQ=38)
                                                                         tAGGGCAGATAAGGCGT‑‑TCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCcaca              <  1:30479/93‑1 (MQ=38)
                                                                         tAGGGCAGATAAGGCGT‑‑TCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCcaca              >  1:860599/1‑93 (MQ=38)
                                                                             gCAGATAAGGCGT‑‑TCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCa          <  1:3193700/93‑1 (MQ=38)
                                                                             gCAGATAAGGCGT‑‑TCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCa          <  1:913498/93‑1 (MQ=38)
                                                                             gCAGATAAGGCGT‑‑TCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCa          >  2:1418035/1‑93 (MQ=38)
                                                                              cAGATAAGGCGT‑‑TCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCaa         >  1:557621/1‑93 (MQ=38)
                                                                              cAGATAAGGCGT‑‑TCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCaa         >  1:6087012/1‑93 (MQ=38)
                                                                                gATAAGGCGT‑‑TCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAAc       >  1:2866376/1‑93 (MQ=39)
                                                                                gATAAGGCGT‑‑TCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAAc       >  2:2339150/1‑93 (MQ=39)
                                                                                 aTAAGGCGT‑‑TCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACt      <  1:437682/93‑1 (MQ=38)
                                                                                  tAAGGCGT‑‑TCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTc     >  1:5390653/1‑93 (MQ=39)
                                                                                   aaGGCGT‑‑TCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTTCACAGCCAAACTCa    >  2:2521591/1‑93 (MQ=39)
                                                                                   aaGGCGT‑‑TCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCa    >  1:5040083/1‑93 (MQ=39)
                                                                                     ggCGT‑‑TCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCACTTTTTTTTCCCAGCCAAACTCcta  >  2:1203590/1‑93 (MQ=255)
                                                                                               ||                                                                                   
GCGCTGGCGGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGT‑‑TCA‑‑CCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATA  >  NC_000913/3537312‑3537487

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCGCTGGCGGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCA‑‑CCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCG  >  NC_000913/3537312‑3537497
                                                                                               |                                                                                            
GCGCTGGCGGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGTCAGATAAGGCGTTCA‑‑C                                                                                              <  SRR4302537.3206757/94‑1 (MQ=60)
GCGCTGGCGGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCA‑‑C                                                                                              <  SRR4302537.868737/94‑1 (MQ=60)
GCGCTGGCGGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCA‑‑C                                                                                              <  SRR4302537.4190910/94‑1 (MQ=60)
 CGCTGGCGGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCA‑‑CG                                                                                             <  SRR4302537.2707903/94‑1 (MQ=60)
   CTGGCGGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCA‑‑Cgcc                                                                                           >  SRR4302537.5869813/1‑91 (MQ=60)
       CGGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCAT                                                                                         <  SRR4302537.3503994/94‑1 (MQ=60)
          CGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTG                                                                                      >  SRR4302537.253766/1‑94 (MQ=60)
          CGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCA‑‑Cgccgcacctg                                                                                    >  SRR4302537.3538825/1‑84 (MQ=60)
           GGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGG                                                                                     <  SRR4302537.821582/94‑1 (MQ=60)
            GCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGC                                                                                    >  SRR4302537.2704271/1‑94 (MQ=60)
             CAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCA                                                                                   >  SRR4302537.3822871/1‑94 (MQ=60)
             CAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCA                                                                                   >  SRR4302537.2210958/1‑94 (MQ=60)
             CAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCA                                                                                   >  SRR4302537.2262260/1‑94 (MQ=60)
             CAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCA                                                                                   >  SRR4302537.786222/1‑94 (MQ=60)
             CAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCA                                                                                   >  SRR4302537.2303173/1‑94 (MQ=60)
              AATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAA                                                                                  >  SRR4302537.4495548/1‑94 (MQ=60)
               ATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAAC                                                                                 >  SRR4302537.1604153/1‑94 (MQ=60)
               ATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAAC                                                                                 >  SRR4302537.3797093/1‑94 (MQ=60)
                  GGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAA                                                                              >  SRR4302537.3964922/1‑94 (MQ=60)
                  GGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAA                                                                              <  SRR4302537.679732/94‑1 (MQ=60)
                  GGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAA                                                                              <  SRR4302537.6121165/94‑1 (MQ=60)
                   GCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAAC                                                                             <  SRR4302537.2555442/94‑1 (MQ=60)
                   GCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAAC                                                                             <  SRR4302537.2717706/94‑1 (MQ=60)
                   GCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAAC                                                                             >  SRR4302537.5566636/1‑94 (MQ=60)
                   GCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAAC                                                                             >  SRR4302537.4368626/1‑94 (MQ=60)
                   GCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAAC                                                                             <  SRR4302537.5198626/94‑1 (MQ=60)
                    CAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACG                                                                            >  SRR4302537.2146413/1‑94 (MQ=60)
                     AAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGC                                                                           >  SRR4302537.6149582/1‑94 (MQ=60)
                     AAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGCGGTTCACGCCGCATCTGGCAACGAACGC                                                                           >  SRR4302537.6152694/1‑94 (MQ=60)
                       AAAACGTTAAACGCCCGTAGTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCT                                                                         <  SRR4302537.1378618/94‑1 (MQ=60)
                       AAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCT                                                                         <  SRR4302537.2041054/94‑1 (MQ=60)
                        AAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTT                                                                        <  SRR4302537.1758511/94‑1 (MQ=60)
                        AAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTT                                                                        <  SRR4302537.649821/94‑1 (MQ=60)
                        AAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAACGCGTTCACGCAGCATCTGGCAACGAACGCCTT                                                                        >  SRR4302537.465167/1‑94 (MQ=60)
                         AACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTG                                                                       >  SRR4302537.2137988/1‑94 (MQ=60)
                         AACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTG                                                                       >  SRR4302537.1480711/1‑94 (MQ=60)
                         AACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTG                                                                       >  SRR4302537.3565025/1‑94 (MQ=60)
                           CGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCC                                                                     >  SRR4302537.5290397/1‑94 (MQ=60)
                            GTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCT                                                                    >  SRR4302537.5660384/1‑94 (MQ=60)
                            GTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCT                                                                    <  SRR4302537.5820355/94‑1 (MQ=60)
                              TAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGA                                                                  >  SRR4302537.5320127/1‑94 (MQ=60)
                                AACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACA                                                                <  SRR4302537.4066709/94‑1 (MQ=60)
                                    CCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAA                                                            <  SRR4302537.601428/94‑1 (MQ=60)
                                    CCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAA                                                            <  SRR4302537.601429/94‑1 (MQ=60)
                                     CCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAA                                                           >  SRR4302537.5120408/1‑94 (MQ=60)
                                      CGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAG                                                          <  SRR4302537.1599103/94‑1 (MQ=60)
                                       GTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGT                                                         >  SRR4302537.5483398/1‑94 (MQ=60)
                                       GTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGT                                                         >  SRR4302537.4052271/1‑94 (MQ=60)
                                        TACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTG                                                        <  SRR4302537.3181425/94‑1 (MQ=60)
                                        TACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTG                                                        >  SRR4302537.5180932/1‑94 (MQ=60)
                                        TACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTG                                                        <  SRR4302537.1083883/94‑1 (MQ=60)
                                        TACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTG                                                        <  SRR4302537.1710139/94‑1 (MQ=60)
                                         TCTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGC                                                       <  SRR4302537.1446032/94‑1 (MQ=60)
                                         TCTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGC                                                       <  SRR4302537.211364/94‑1 (MQ=60)
                                         ACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGC                                                       >  SRR4302537.3774936/1‑94 (MQ=60)
                                         ACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGC                                                       >  SRR4302537.3261940/1‑94 (MQ=60)
                                          CTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCC                                                      >  SRR4302537.6245679/1‑94 (MQ=60)
                                          CTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCC                                                      >  SRR4302537.2119460/1‑94 (MQ=60)
                                          CTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCC                                                      >  SRR4302537.918001/1‑94 (MQ=60)
                                          CTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCC                                                      >  SRR4302537.2398847/1‑94 (MQ=60)
                                           TGGCCTACGGTTCGAGTTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCG                                                     <  SRR4302537.259000/94‑1 (MQ=60)
                                           TGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCG                                                     <  SRR4302537.4213264/94‑1 (MQ=60)
                                           TGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCG                                                     >  SRR4302537.541161/1‑94 (MQ=60)
                                            GGCCTACGCTTCGAATTTGCACAAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGG                                                    <  SRR4302537.175518/94‑1 (MQ=60)
                                             GCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGTCGGA                                                   >  SRR4302537.1203319/1‑94 (MQ=60)
                                             GCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGA                                                   >  SRR4302537.2033691/1‑94 (MQ=60)
                                              CCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAG                                                  <  SRR4302537.3366255/94‑1 (MQ=60)
                                              CCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAG                                                  >  SRR4302537.2651166/1‑94 (MQ=60)
                                              CCTACGGTTCGAATTTGCACGAAATCGTAGAGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAG                                                  >  SRR4302537.3609256/1‑94 (MQ=60)
                                               CTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGA                                                 >  SRR4302537.1970255/1‑94 (MQ=60)
                                               CTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGA                                                 >  SRR4302537.5049652/1‑94 (MQ=60)
                                                 ACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAAT                                               <  SRR4302537.3399177/94‑1 (MQ=60)
                                                  CGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATA                                              >  SRR4302537.2689910/1‑94 (MQ=60)
                                                  CGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATA                                              <  SRR4302537.2838203/94‑1 (MQ=60)
                                                  CGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATA                                              <  SRR4302537.2242536/94‑1 (MQ=60)
                                                   GGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATAT                                             >  SRR4302537.4962609/1‑94 (MQ=60)
                                                   GGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATAT                                             >  SRR4302537.2773152/1‑94 (MQ=60)
                                                    GTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATC                                            <  SRR4302537.2123055/94‑1 (MQ=60)
                                                    GTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATC                                            >  SRR4302537.4856176/1‑94 (MQ=60)
                                                    GTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATC                                            >  SRR4302537.3036560/1‑94 (MQ=60)
                                                     TTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCT                                           <  SRR4302537.840793/94‑1 (MQ=60)
                                                      TCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTC                                          >  SRR4302537.5881847/1‑94 (MQ=60)
                                                      TCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTC                                          <  SRR4302537.4326183/94‑1 (MQ=60)
                                                       CGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCC                                         >  SRR4302537.5264852/1‑94 (MQ=60)
                                                        GAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCG                                        >  SRR4302537.3204533/1‑94 (MQ=60)
                                                        GAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCG                                        <  SRR4302537.5258865/94‑1 (MQ=60)
                                                        GAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCG                                        >  SRR4302537.2098254/1‑94 (MQ=60)
                                                        GAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCG                                        <  SRR4302537.3248768/94‑1 (MQ=60)
                                                         AATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGG                                       <  SRR4302537.4152625/94‑1 (MQ=60)
                                                          ATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGC                                      >  SRR4302537.1866530/1‑94 (MQ=60)
                                                          ATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGC                                      >  SRR4302537.1319746/1‑94 (MQ=60)
                                                           TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCA                                     >  SRR4302537.5080203/1‑94 (MQ=60)
                                                           TTTGCACAAAAGCTTAGGGCAGATAAGGCGTCCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCA                                     <  SRR4302537.508173/94‑1 (MQ=60)
                                                            TTGCACGAAATCGTAGGGGAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCAT                                    <  SRR4302537.5087149/94‑1 (MQ=60)
                                                            TTGCACGAAATCGTAGAGCAGATAAGCCTTCCACGCCGCATCTGGCAACGAACTCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCAT                                    <  SRR4302537.5087150/94‑1 (MQ=60)
                                                                ACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTT                                <  SRR4302537.4190334/94‑1 (MQ=60)
                                                                 CGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTA                               >  SRR4302537.1979887/1‑94 (MQ=60)
                                                                  GAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTAT                              >  SRR4302537.2664638/1‑94 (MQ=60)
                                                                  GAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTAT                              <  SRR4302537.3771386/94‑1 (MQ=60)
                                                                   AAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTTTT                             >  SRR4302537.2363485/1‑94 (MQ=60)
                                                                    AATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTC                            >  SRR4302537.519118/1‑94 (MQ=60)
                                                                    AATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTC                            <  SRR4302537.4361641/94‑1 (MQ=60)
                                                                    AATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTC                            >  SRR4302537.3085149/1‑94 (MQ=60)
                                                                     ATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAGCGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCC                           >  SRR4302537.5196204/1‑94 (MQ=60)
                                                                      TCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGGATATCTCCTGCATTTTTATTCCA                          >  SRR4302537.5137580/1‑94 (MQ=60)
                                                                       CGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCAC                         <  SRR4302537.4625659/94‑1 (MQ=60)
                                                                       CGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCAC                         <  SRR4302537.302480/94‑1 (MQ=60)
                                                                        GTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACA                        >  SRR4302537.866501/1‑94 (MQ=60)
                                                                        GTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACA                        <  SRR4302537.4328073/94‑1 (MQ=60)
                                                                         TAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAG                       <  SRR4302537.104756/94‑1 (MQ=60)
                                                                         TAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAG                       >  SRR4302537.4380611/1‑94 (MQ=60)
                                                                         TAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAG                       <  SRR4302537.30723/94‑1 (MQ=60)
                                                                          AGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGC                      >  SRR4302537.3849615/1‑94 (MQ=60)
                                                                          AGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGC                      <  SRR4302537.3259436/94‑1 (MQ=60)
                                                                             GCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAA                   >  SRR4302537.6128830/1‑94 (MQ=60)
                                                                             GCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAA                   <  SRR4302537.3216158/94‑1 (MQ=60)
                                                                             GCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAA                   >  SRR4302537.561462/1‑94 (MQ=60)
                                                                             GCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAA                   <  SRR4302537.919804/94‑1 (MQ=60)
                                                                              CAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAA                  >  SRR4302537.6499762/1‑94 (MQ=60)
                                                                              CAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGACATTTTTATTCCACAGCCAAA                  >  SRR4302537.5634543/1‑94 (MQ=60)
                                                                               AGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAAC                 >  SRR4302537.593212/1‑94 (MQ=60)
                                                                               AGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAAC                 >  SRR4302537.972620/1‑94 (MQ=60)
                                                                               AGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAAC                 <  SRR4302537.2673578/94‑1 (MQ=60)
                                                                               AGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAAC                 >  SRR4302537.2886482/1‑94 (MQ=60)
                                                                                 ATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTC               >  SRR4302537.5427480/1‑94 (MQ=60)
                                                                                 ATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTC               >  SRR4302537.1205487/1‑94 (MQ=60)
                                                                                 ATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTC               <  SRR4302537.440732/94‑1 (MQ=60)
                                                                                  TAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTTCACAGCCAAACTCA              >  SRR4302537.4538324/1‑94 (MQ=60)
                                                                                  TAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCA              <  SRR4302537.5189662/94‑1 (MQ=60)
                                                                                  TAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCA              >  SRR4302537.5074784/1‑94 (MQ=60)
                                                                                   AAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCAT             <  SRR4302537.1387263/94‑1 (MQ=60)
                                                                                    AGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATA            >  SRR4302537.1402645/1‑94 (MQ=60)
                                                                                    AGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATA            >  SRR4302537.6329248/1‑94 (MQ=60)
                                                                                     GGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAA           <  SRR4302537.5862021/94‑1 (MQ=60)
                                                                                      GCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAAT          >  SRR4302537.6129319/1‑94 (MQ=60)
                                                                                      GCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAAT          >  SRR4302537.1304661/1‑94 (MQ=60)
                                                                                      GCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAAT          <  SRR4302537.1592471/94‑1 (MQ=60)
                                                                                        GTTCACGCCGCATCTGGCAACGAACGCCTTTCCTGACATAAAAGTGCCGGAGAATCTCTCCGGCATTTTTATTCCACAGCCACACTCATAATAT        >  SRR4302537.4724344/1‑94 (MQ=60)
                                                                                        GTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATAT        <  SRR4302537.597932/94‑1 (MQ=60)
                                                                                         TTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGACTATCTCCGGCATTTTTATTCCACAGCCACACTCATAATATA       >  SRR4302537.2591452/1‑94 (MQ=60)
                                                                                         TTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATA       >  SRR4302537.4390713/1‑94 (MQ=60)
                                                                                          tcacgCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATAT      <  SRR4302537.1662083/89‑1 (MQ=60)
                                                                                           cacgCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATT     >  SRR4302537.4107471/5‑94 (MQ=60)
                                                                                           cacgCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATT     <  SRR4302537.4570913/90‑1 (MQ=60)
                                                                                           cacgCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATT     <  SRR4302537.6366217/90‑1 (MQ=60)
                                                                                           cacgCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGACGGAGAATATCTCCGGCATTTTTATTCCACAGACAAACTCATAATATATT     >  SRR4302537.2074233/5‑94 (MQ=60)
                                                                                           CG‑‑CCGCATCTGGCAACGAACTCCTTGCCTGACATAAAATTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCC   >  SRR4302537.5094385/1‑94 (MQ=60)
                                                                                           CG‑‑CCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCC   >  SRR4302537.1320043/1‑94 (MQ=60)
                                                                                           CG‑‑CCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCC   <  SRR4302537.1013732/94‑1 (MQ=60)
                                                                                           CG‑‑CCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCC   <  SRR4302537.4935392/94‑1 (MQ=60)
                                                                                            acgCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTC    <  SRR4302537.6419859/91‑1 (MQ=60)
                                                                                            G‑‑CCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCG  >  SRR4302537.3855349/1‑94 (MQ=60)
                                                                                            G‑‑CCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCG  <  SRR4302537.4060861/94‑1 (MQ=60)
                                                                                            G‑‑CCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCG  >  SRR4302537.5940730/1‑94 (MQ=60)
                                                                                               |                                                                                            
GCGCTGGCGGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCA‑‑CCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCG  >  NC_000913/3537312‑3537497

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑