Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 647 270.1 21258240 98.4% 20918108 94.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,544,363 +C coding (169/1314 nt) gntT → gluconate permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,544,3631.C99.8% 1538.9 / ‑4.7 452coding (169/1314 nt)gntTgluconate permease
Reads supporting (aligned to +/- strand):  ref base . (1/0);  new base C (193/258);  total (194/258)
Fisher's exact test for biased strand distribution p-value = 4.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 467 total aligned reads displayed.

CTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTC‑G‑‑C‑G‑GACG‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACAAC  >  NC_000913/3544282‑3544438
                                                                                       |                                                                           
  cGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGG‑GACGCTCGGTAGCCtt                                                                  >  1:8159304/1‑94 (MQ=255)
  cGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGG‑GACGCTCGGTAGCCtt                                                                  <  2:6153694/94‑1 (MQ=255)
  cGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGG‑GACGCTCGGTAGCCtt                                                                  <  2:4709306/94‑1 (MQ=255)
  cGTGGCGCTTCCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGG‑GACGCTCGGTAGCCtt                                                                  >  1:7047413/1‑94 (MQ=255)
   gtgtCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGG‑GACGCTCGGTAGCCTTg                                                                 <  1:4504794/94‑1 (MQ=255)
   gTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGG‑GACGCTCGGTAGCCTTg                                                                 <  2:1080010/94‑1 (MQ=255)
      gcgcTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGG‑GACGCTCGGTAGCCTTGccc                                                              <  1:8687972/94‑1 (MQ=255)
       cgcTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGG‑GACGCTCGGTAGCCTTGCCCt                                                             <  1:4401126/94‑1 (MQ=255)
       cgcTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGG‑GACGCTCGGTAGCCTTGCCCt                                                             <  1:9999527/94‑1 (MQ=255)
        gcTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGG‑GACGCTCGGTAGCCTTGCCCTg                                                            <  2:2221001/94‑1 (MQ=255)
        gcTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGG‑GACGCTCGGTAGCCTTGCCCTg                                                            >  2:2484894/1‑94 (MQ=255)
        gcTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGG‑GACGCTCGGTAGCCTTGCCCTg                                                            >  1:1845838/1‑94 (MQ=255)
         cTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGG‑GACGCTCGGTAGCCTTGCCCTGa                                                           >  2:930033/1‑94 (MQ=255)
         cTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGG‑GACGCTCGGTAGCCTTGCCCTGa                                                           <  2:1898193/94‑1 (MQ=255)
           tGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGG‑GACGCTCGGTAGCCTTGCCCTGATc                                                         <  1:8261707/94‑1 (MQ=255)
           tGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGG‑GACGCTCGGTAGCCTTGCCCTGATc                                                         >  1:1055975/1‑94 (MQ=255)
            gCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGG‑GACGCTCGGTAGCCTTGCCCTGATCa                                                        <  1:8523585/94‑1 (MQ=255)
             cTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGG‑GACGCTCGGTAGCCTTGCCCTGATCAt                                                       <  2:5011411/94‑1 (MQ=255)
             cTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGG‑GACGCTCGGTAGCCTTGCCCTGATCAt                                                       >  1:2265179/1‑94 (MQ=255)
             cTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGG‑GACGCTCGGTAGCCTTGCCCTGATCAt                                                       <  1:2113059/94‑1 (MQ=255)
             cTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGG‑GACGCTCGGTAGCCTTGCCCTGATCAt                                                       <  2:4346245/94‑1 (MQ=255)
             cTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGG‑GACGCTCGGTAGCCTTGCCCTGATCAt                                                       >  1:6893887/1‑94 (MQ=255)
             cTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGG‑GACGCTCGGTAGCCTTGCCCTGATCAt                                                       >  1:6354153/1‑94 (MQ=255)
              tGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGG‑GACGCTCGGTAGCCTTGCCCTGATCATg                                                      <  2:8145693/94‑1 (MQ=255)
               gTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGG‑GACGCTCGGTAGCCTTGCCCTGATCATgg                                                     <  1:1499795/94‑1 (MQ=255)
               gTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGG‑GACGCTCGGTAGCCTTGCCCTGATCATgg                                                     >  2:641168/1‑94 (MQ=255)
                   gATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGG‑GACGCTCGGTAGCCTTGCCCTGATCATGGGttt                                                 <  2:5335294/94‑1 (MQ=255)
                   gATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGG‑GACGCTCGGTAGCCTTGCCCTGATCATGGGttt                                                 <  1:4920468/94‑1 (MQ=255)
                    agtAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGG‑GACGCTCGGTAGCCTTGCCCTGATCATGGGtttt                                                <  2:3094193/92‑1 (MQ=255)
                    aTTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGG‑GACGCTCGGTAGCCTTGCCCTGATCATGGGtttt                                                <  1:5693184/94‑1 (MQ=255)
                     ttAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGG‑GACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTg                                               <  2:8804088/94‑1 (MQ=255)
                     ttAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGG‑GACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTg                                               <  1:9177824/94‑1 (MQ=255)
                      tAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGG‑GACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTgg                                              <  1:4292407/94‑1 (MQ=255)
                      tAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGG‑GACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTgg                                              >  1:7418378/1‑94 (MQ=255)
                      tAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGG‑GACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTgg                                              <  1:974410/94‑1 (MQ=255)
                        aTGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGG‑GACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGgcg                                            <  2:7734522/94‑1 (MQ=255)
                         tGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGG‑GACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGgcgc                                           >  1:3885738/1‑94 (MQ=255)
                         tGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGG‑GACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGgcgc                                           >  2:10310235/1‑94 (MQ=255)
                         tGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGG‑GACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGgcgc                                           >  1:10065807/1‑94 (MQ=255)
                         tGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGG‑GACGCTCGGTAGCCTTGCCCTGATCATGGGGTTTGgcgc                                           >  1:9512227/1‑94 (MQ=255)
                            aaGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGG‑GACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAAt                                        >  1:2501078/1‑94 (MQ=255)
                            aaGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGG‑GACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAAt                                        >  1:5220077/1‑94 (MQ=255)
                            aaGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGG‑GACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAAt                                        <  1:1360256/94‑1 (MQ=255)
                            aaGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGG‑GACGCTCGGGAGCCTTGCCCTGATCATGGGTTTTGGCGCAAt                                        <  2:4515060/94‑1 (MQ=255)
                             aGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGG‑GACGCCCGGCAGCCTTGCCCTGATCAGGGGTTTTGGCGCaacg                                       >  2:7111627/1‑92 (MQ=255)
                               gAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGG‑GACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCt                                     <  2:833379/94‑1 (MQ=255)
                               gAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGG‑GACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCt                                     <  2:5699202/94‑1 (MQ=255)
                               gAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGG‑GACGCTCGGTAGCCTTGCCCTGATCATGGGATTTGGCGCAATGCt                                     <  1:1022944/94‑1 (MQ=255)
                                 aTGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGG‑GACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTgg                                   >  2:7265182/1‑94 (MQ=255)
                                 aTGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGG‑GACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTgg                                   >  2:8548609/1‑94 (MQ=255)
                                 aTGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGG‑GACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTgg                                   >  2:4064718/1‑94 (MQ=255)
                                   gCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGG‑GACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGc                                 <  2:438094/94‑1 (MQ=255)
                                    gcGCTGCATAAAGTTATTGGCTCAATCAAAGCCGGTGTCGG‑‑CGG‑GACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCa                                <  2:6026233/93‑1 (MQ=255)
                                    ccGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGG‑GACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCa                                <  1:6710955/94‑1 (MQ=255)
                                    ccGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGG‑GACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCa                                >  2:8929677/1‑94 (MQ=255)
                                     cGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGG‑GACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCaa                               >  1:3398624/1‑94 (MQ=255)
                                      gCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGG‑GACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCaaa                              >  1:7974060/1‑94 (MQ=255)
                                      gCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGG‑GACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCaaa                              <  2:2140292/94‑1 (MQ=255)
                                       cTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGG‑GACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCacaa                             >  2:3935816/1‑94 (MQ=255)
                                       cTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGG‑GACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCaaaa                             <  2:7038103/94‑1 (MQ=255)
                                       cTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGG‑GACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGCTGCAATGCTGGGCaaaa                             >  2:2657469/1‑94 (MQ=255)
                                        tGGATAAAGTTATTGGCTCCATCAGTGCCGGTGTCGG‑‑CGG‑GACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAAt                            <  1:1095399/94‑1 (MQ=255)
                                        tGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGG‑GACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAAt                            >  1:550589/1‑94 (MQ=255)
                                         ggATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGG‑GACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATg                           >  1:2956387/1‑94 (MQ=255)
                                         ggATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGG‑GACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATg                           <  1:4663201/94‑1 (MQ=255)
                                         ggATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGG‑GACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATg                           <  1:3090587/94‑1 (MQ=255)
                                          gATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGG‑GACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGc                          >  1:6056936/1‑94 (MQ=255)
                                          gATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGG‑GACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGc                          >  2:6178379/1‑94 (MQ=255)
                                           aTAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGG‑GACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCt                         >  1:7250820/1‑94 (MQ=255)
                                            tacaGTAATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGG‑GACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTg                        <  2:4571897/91‑1 (MQ=255)
                                            aaaaGTTATTGGCTCCATCAANACCGGTGTC‑GGCC‑G‑GACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTg                        <  2:3276071/93‑1 (MQ=255)
                                             aaaGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGG‑GACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTgg                       <  2:9481299/94‑1 (MQ=255)
                                             aaaGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGG‑GACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTgg                       <  2:887336/94‑1 (MQ=255)
                                               aGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGG‑GACGCTCGGTAGCCTTGCCCTGATCGTGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCa                     <  2:3465310/94‑1 (MQ=255)
                                               aGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGG‑GACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCa                     <  2:5163675/94‑1 (MQ=255)
                                                gTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGG‑GACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAg                    <  2:236882/94‑1 (MQ=255)
                                                gTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGG‑GACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAg                    <  2:8580613/94‑1 (MQ=255)
                                                  tATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGG‑GACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGAc                  >  2:9224741/1‑94 (MQ=255)
                                                  tATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGG‑GACGCTCGGTAGCCTTGCCCTGATCATGGGGTTTGGCGCAATGCTGGGCAAAATGCTGGCAGAc                  >  2:9746923/1‑94 (MQ=255)
                                                  tATTGGCTCCATCAAAGCCGGTGACGG‑‑CGG‑GACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGAc                  <  1:5840960/94‑1 (MQ=255)
                                                     tGGCTCCATCAAAGCCGGTGTCGG‑‑CGG‑GACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGc               <  1:7645889/94‑1 (MQ=255)
                                                     tGGCTCCATCAAAGCCGGTGTCGG‑‑CGG‑GACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGc               <  1:9309698/94‑1 (MQ=255)
                                                     tGGCTCCATCAAAGCCGGTGTCGG‑‑CGG‑GACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGc               >  2:7601937/1‑94 (MQ=255)
                                                      ggCTCCATCAAAGCCGGTGTCGG‑‑CGG‑GACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCg              <  1:10388920/94‑1 (MQ=255)
                                                      ggCTCCATCAAAGCCGGTGTCGG‑‑CGG‑GACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCg              >  2:6786021/1‑94 (MQ=255)
                                                       gCTCCATCAAAGCCGGTGTCGG‑‑CGG‑GACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCgg             <  1:159941/94‑1 (MQ=255)
                                                        cTCCATCAAAGCCGGTGTCGG‑‑CGG‑GACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCggg            >  2:7886500/1‑93 (MQ=255)
                                                         tCCATCAAAGCCGGTGTCGG‑‑CGG‑GACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTg           <  1:8393719/94‑1 (MQ=255)
                                                         tCCATCAAAGCCGGTGTCGG‑‑CGG‑GACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTg           <  1:6284039/94‑1 (MQ=255)
                                                          ccATCAAAGCCGGTGTCGG‑‑CGG‑GACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTgg          <  1:3692491/94‑1 (MQ=255)
                                                           cATCAAAGCCGGTGTCGG‑‑CGG‑GACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGgc         >  2:6725796/1‑94 (MQ=255)
                                                           cATCAAAGCCGGTGTCGG‑‑CGG‑GACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGgc         <  1:5397795/94‑1 (MQ=255)
                                                           cATCAAAGCCGGTGTCGG‑‑CGG‑GACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGgc         >  1:5289123/1‑94 (MQ=255)
                                                             tCAAAGCCGGTGTCGG‑‑CGG‑GACGCTCGGTAGCCTTGCCCTGATCATTGGTGTTGGCGCAATGCTTGGCAAAATGCTGGCAGTCTGCGGTGgcgc       >  1:9385380/1‑94 (MQ=255)
                                                               aaaGCCGGTGTCGG‑‑T‑GTGACGCTCGGTAGGCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGcac     <  2:5737062/94‑1 (MQ=255)
                                                               aaaGCCGGTGTCGG‑‑CGG‑GACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGcac     >  2:1698513/1‑94 (MQ=255)
                                                               aaaGCCGGTGTCGG‑‑CGG‑GACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGcac     >  2:3379218/1‑94 (MQ=255)
                                                               aaaGCCGGTGTCGG‑‑CGG‑GACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGcac     >  1:3554828/1‑94 (MQ=255)
                                                                aaGCCGGTGTCGG‑‑CGG‑GACGCTCGGTAGCCTTGCCCTGATCATGGGGTTTGGCGCAATGCAGGGCAAAATGCTGGCAGACTGCGGTGGCGcaca    >  2:10224127/1‑94 (MQ=255)
                                                                 aGCCGGTGTCGG‑‑CGG‑GACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACaa   >  2:1281811/1‑94 (MQ=255)
                                                                                       |                                                                           
CTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTC‑G‑‑C‑G‑GACG‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACAAC  >  NC_000913/3544282‑3544438

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 354 total aligned reads displayed.
CCGATACTGTGCAGCATCTGGTCGAAGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTACCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGACCAAAAATAAAGATATTTTCTTCAC  >  NC_000913/3547912‑3548098
                                                                                             |                                                                                             
 CGATACTGTGCAGCATCTGGTCGAAGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATC                                                                                              <  SRR4302545.6978596/94‑1 (MQ=60)
  GATACTGTGCAGCATCTGGTCGAAGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCT                                                                                             >  SRR4302545.137402/1‑94 (MQ=60)
  GATACTGTGCAGCATCTGGTCGAAGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCT                                                                                             <  SRR4302545.1053146/94‑1 (MQ=60)
  GATACTGTGCAGCATCTGGTCGAAGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCT                                                                                             <  SRR4302545.320418/94‑1 (MQ=60)
   ATACTGTGCAGCATCTGGTCGAAGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTT                                                                                            >  SRR4302545.10004095/1‑94 (MQ=60)
   ATACTGTGCAGCATCTGGTCGAAGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTT                                                                                            >  SRR4302545.4821140/1‑94 (MQ=60)
   ATACTGTGCAGCATCTGGTCGAAGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTT                                                                                            >  SRR4302545.2786388/1‑94 (MQ=60)
   ATACTGTGCAGCATCTGGTCGAAGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTT                                                                                            >  SRR4302545.466701/1‑94 (MQ=60)
   ATACTGTGCAGCATCTGGTCGAAGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTT                                                                                            >  SRR4302545.7064881/1‑94 (MQ=60)
   ATACTGTGCAGCATCTGGTCGAAGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTT                                                                                            <  SRR4302545.6266410/94‑1 (MQ=60)
    TACTGTGCAGCATCTGGTCGAAGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTT                                                                                           >  SRR4302545.7957276/1‑94 (MQ=60)
      CTGTGCAGCATCTGGTCGAAGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCT                                                                                         <  SRR4302545.9937210/94‑1 (MQ=60)
      CTGTGCAGCATCTGGTCGAAGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCT                                                                                         >  SRR4302545.7747721/1‑94 (MQ=60)
      CTGTGCAGCATCTGGTCGAAGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCT                                                                                         >  SRR4302545.3944954/1‑94 (MQ=60)
       TGTGCAGCATCTGGTCGAAGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTT                                                                                        <  SRR4302545.8168146/94‑1 (MQ=60)
        GTGCAGCATCTGGTCGAAGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTC                                                                                       >  SRR4302545.8595911/1‑94 (MQ=60)
        GTGCAGCATCTGGTCGAAGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTC                                                                                       <  SRR4302545.5396477/94‑1 (MQ=60)
         TGCAGCATCTGGTCGAAGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCC                                                                                      >  SRR4302545.170580/1‑94 (MQ=60)
         TGCAGCATCTGGTCGAAGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTGCC                                                                                      >  SRR4302545.4524772/1‑94 (MQ=60)
         TGCAGCATCTGGTCGAAGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGT‑CAGCACCTTATCTTTCTTTCG                                                                                     >  SRR4302545.145871/1‑94 (MQ=60)
          ACAGCATATGGTAGACGGCATGCTTATCGCCGTCACTGTATTTACCGCTTACCAGCTCTTTCAATACTGCGTCCAGCACATTATCTTTCTTCCG                                                                                     <  SRR4302545.9627389/94‑1 (MQ=60)
           CAGCATCTGGTCGAAGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGC                                                                                    >  SRR4302545.10517719/1‑94 (MQ=60)
           CAGCATCTGGTCGAAGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGC                                                                                    >  SRR4302545.4948059/1‑94 (MQ=60)
           CAGCATCTGGTCGAAGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGC                                                                                    >  SRR4302545.5940825/1‑94 (MQ=60)
            AGCATCTGGTCGAAGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCC                                                                                   >  SRR4302545.1212513/1‑94 (MQ=60)
            AGCATCTGGTCGAAGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCC                                                                                   >  SRR4302545.1212514/1‑94 (MQ=60)
              CATCTGGTCGAAGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCAT                                                                                 >  SRR4302545.203317/1‑94 (MQ=60)
               ATCTGGTCGAAGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATT                                                                                >  SRR4302545.3891092/1‑94 (MQ=60)
               ATCTGGTCGAAGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATT                                                                                <  SRR4302545.4484513/94‑1 (MQ=60)
               ATCTGGTCGAAGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATT                                                                                >  SRR4302545.10149608/1‑94 (MQ=60)
                  TGGTCGAAGGAATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCA                                                                             >  SRR4302545.2429114/1‑94 (MQ=60)
                   GGTCGAAGGCATGCTTATCGCCGTCGCTTTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCAC                                                                            <  SRR4302545.5724685/94‑1 (MQ=60)
                    GTCGAAGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACC                                                                           >  SRR4302545.6592446/1‑94 (MQ=60)
                    GTCGAAGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACC                                                                           <  SRR4302545.5738810/94‑1 (MQ=60)
                    GTCGAAGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACC                                                                           >  SRR4302545.10220233/1‑94 (MQ=60)
                    GTCGAAGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCACCCCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACC                                                                           <  SRR4302545.9326756/94‑1 (MQ=60)
                     TCGAAGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCG                                                                          <  SRR4302545.3365920/94‑1 (MQ=60)
                      CGAAGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGG                                                                         <  SRR4302545.2339826/94‑1 (MQ=60)
                      CGAAGGCATGCTTATCGCCGTCGATGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGG                                                                         <  SRR4302545.6004660/94‑1 (MQ=60)
                       GAAGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGG                                                                        >  SRR4302545.6699037/1‑94 (MQ=60)
                       GAAGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGG                                                                        >  SRR4302545.1370701/1‑94 (MQ=60)
                        AATGCATGCTTATCGCCGTCTCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGT                                                                       <  SRR4302545.5807086/94‑1 (MQ=60)
                        AAGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCAGCTTATGTTTCTTCCGCCATTTCACCGGGT                                                                       >  SRR4302545.6049564/1‑94 (MQ=60)
                        AAGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGT                                                                       <  SRR4302545.4705243/94‑1 (MQ=60)
                        AAGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGT                                                                       >  SRR4302545.7513371/1‑94 (MQ=60)
                          GGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCG                                                                     <  SRR4302545.3532766/94‑1 (MQ=60)
                          GGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCG                                                                     <  SRR4302545.2946522/94‑1 (MQ=60)
                           GCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGT                                                                    <  SRR4302545.5506003/94‑1 (MQ=60)
                           GCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGT                                                                    <  SRR4302545.1418984/94‑1 (MQ=60)
                            CATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTA                                                                   >  SRR4302545.918662/1‑94 (MQ=60)
                            CATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTA                                                                   >  SRR4302545.4569171/1‑94 (MQ=60)
                            CATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTA                                                                   <  SRR4302545.1485964/94‑1 (MQ=60)
                            CATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTA                                                                   <  SRR4302545.9321556/94‑1 (MQ=60)
                             ATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAG                                                                  >  SRR4302545.3749092/1‑94 (MQ=60)
                                CTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCT                                                               >  SRR4302545.2084215/1‑94 (MQ=60)
                                CTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCT                                                               >  SRR4302545.10935843/1‑94 (MQ=60)
                                CTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCT                                                               <  SRR4302545.6128996/94‑1 (MQ=60)
                                CTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCT                                                               <  SRR4302545.8459052/94‑1 (MQ=60)
                                CTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCT                                                               <  SRR4302545.5635274/94‑1 (MQ=60)
                                 TTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGCGTCGTTGCCTT                                                              >  SRR4302545.1467220/1‑94 (MQ=60)
                                 TTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTACAGCACCTTATCTTTCTTCCGCCATTTCTCCGTGTCGCAGCCTT                                                              >  SRR4302545.1910285/1‑94 (MQ=60)
                                    TCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGG                                                           >  SRR4302545.10866243/1‑94 (MQ=60)
                                     CGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGC                                                          <  SRR4302545.6414632/94‑1 (MQ=60)
                                     CGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGC                                                          <  SRR4302545.10829153/94‑1 (MQ=60)
                                      GCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCC                                                         >  SRR4302545.6547008/1‑94 (MQ=60)
                                      GCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCC                                                         >  SRR4302545.7257418/1‑94 (MQ=60)
                                      GCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCC                                                         >  SRR4302545.2370635/1‑94 (MQ=60)
                                       CCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCA                                                        >  SRR4302545.2742025/1‑94 (MQ=60)
                                       CCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCA                                                        >  SRR4302545.2213689/1‑94 (MQ=60)
                                        CGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAG                                                       <  SRR4302545.4645495/94‑1 (MQ=60)
                                        CGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAG                                                       >  SRR4302545.4048791/1‑94 (MQ=60)
                                        CGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAG                                                       <  SRR4302545.8433143/94‑1 (MQ=60)
                                         GTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGA                                                      >  SRR4302545.2501989/1‑94 (MQ=60)
                                          TCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAA                                                     >  SRR4302545.7205769/1‑94 (MQ=60)
                                          GCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAA                                                     <  SRR4302545.514221/94‑1 (MQ=60)
                                           CGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAAT                                                    >  SRR4302545.746913/1‑94 (MQ=60)
                                           CGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAAT                                                    >  SRR4302545.1588249/1‑94 (MQ=60)
                                           CGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCATTTGGCCAGAAT                                                    >  SRR4302545.309177/1‑94 (MQ=60)
                                           CGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGACTTTTGCCAGGAT                                                    >  SRR4302545.10133449/1‑94 (MQ=60)
                                           CGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGACTTTGGaccgcat                                                    >  SRR4302545.130496/1‑87 (MQ=60)
                                           CGCGGTTTTTTCCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACTTTATTTTTCTTCCGCCATTTCACCGGGTCGTTGCCTTTGGTCAGAAT                                                    >  SRR4302545.9688909/1‑94 (MQ=60)
                                            GCTGTATTTCCCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATT                                                   <  SRR4302545.7142941/94‑1 (MQ=60)
                                            GCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATT                                                   <  SRR4302545.3077530/94‑1 (MQ=60)
                                            GCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATT                                                   >  SRR4302545.7989138/1‑94 (MQ=60)
                                            GCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATT                                                   >  SRR4302545.5700526/1‑94 (MQ=60)
                                            GCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATT                                                   >  SRR4302545.6774734/1‑94 (MQ=60)
                                            GCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATT                                                   <  SRR4302545.3339555/94‑1 (MQ=60)
                                             GTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTG                                                  <  SRR4302545.969606/94‑1 (MQ=60)
                                             CTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTG                                                  >  SRR4302545.7173864/1‑94 (MQ=60)
                                             CTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTG                                                  <  SRR4302545.9207346/94‑1 (MQ=60)
                                             CTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTG                                                  >  SRR4302545.2476284/1‑94 (MQ=60)
                                             CTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTG                                                  >  SRR4302545.5515182/1‑94 (MQ=60)
                                              TGTATTTAACGCTTTCCAGCTCTGTCAATACTGCGGCCAGCACCATATCTTTCTTCCGTCATCTCACCGGGTCGTAGCCTTTGGCCCGAAGTGC                                                 >  SRR4302545.9904926/1‑94 (MQ=57)
                                               GTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCC                                                <  SRR4302545.550746/94‑1 (MQ=60)
                                               GTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCC                                                >  SRR4302545.8964578/1‑94 (MQ=60)
                                                TATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCTGGTCGTAGCCTTTGGCCAGAATTGCCT                                               >  SRR4302545.853032/1‑94 (MQ=60)
                                                TATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCT                                               <  SRR4302545.7683499/94‑1 (MQ=60)
                                                 ATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTT                                              >  SRR4302545.5841261/1‑94 (MQ=60)
                                                 ATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTT                                              <  SRR4302545.1853945/94‑1 (MQ=60)
                                                 ATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTT                                              <  SRR4302545.284686/94‑1 (MQ=60)
                                                 ATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTT                                              <  SRR4302545.10808116/94‑1 (MQ=60)
                                                 ATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTT                                              >  SRR4302545.10825319/1‑94 (MQ=60)
                                                 ATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTT                                              >  SRR4302545.3722822/1‑94 (MQ=60)
                                                 ATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTT                                              <  SRR4302545.6869866/94‑1 (MQ=60)
                                                  TTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTC                                             <  SRR4302545.8798208/94‑1 (MQ=60)
                                                  TTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTC                                             <  SRR4302545.2896278/94‑1 (MQ=60)
                                                   TTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCA                                            >  SRR4302545.7904518/1‑94 (MQ=60)
                                                    TACCGCTTTCCAGCTCTTTCAGTACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCAC                                           >  SRR4302545.9430542/1‑94 (MQ=60)
                                                    TACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCAC                                           >  SRR4302545.833474/1‑94 (MQ=60)
                                                    TACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCAC                                           >  SRR4302545.1108640/1‑94 (MQ=60)
                                                     ACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGGCTTCACT                                          >  SRR4302545.6313629/1‑94 (MQ=60)
                                                     ACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACT                                          >  SRR4302545.601085/1‑94 (MQ=60)
                                                      CCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTT                                         <  SRR4302545.2288318/94‑1 (MQ=60)
                                                      CCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTT                                         >  SRR4302545.6512970/1‑94 (MQ=60)
                                                       CGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTG                                        >  SRR4302545.10048151/1‑94 (MQ=60)
                                                        GCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGT                                       <  SRR4302545.1244980/94‑1 (MQ=60)
                                                         CTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTT                                      <  SRR4302545.10637366/94‑1 (MQ=60)
                                                         CTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCATTCTTCCGCCATTTCACCGGGTCGAAGCCTTTGGCCAGAATTGACTTCACTTCTT                                      >  SRR4302545.5886229/1‑94 (MQ=60)
                                                           TTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCC                                    <  SRR4302545.4479880/94‑1 (MQ=60)
                                                           TTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCC                                    >  SRR4302545.8245661/1‑94 (MQ=60)
                                                            TCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCA                                   <  SRR4302545.7991794/94‑1 (MQ=60)
                                                            TCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCA                                   >  SRR4302545.191791/1‑94 (MQ=60)
                                                             CCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCAC                                  <  SRR4302545.2933492/94‑1 (MQ=60)
                                                             CCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCAC                                  >  SRR4302545.2232959/1‑94 (MQ=60)
                                                             CCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCAC                                  >  SRR4302545.3729932/1‑94 (MQ=60)
                                                             CCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCAC                                  >  SRR4302545.134348/1‑94 (MQ=60)
                                                              CAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACG                                 <  SRR4302545.5564464/94‑1 (MQ=60)
                                                              CAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACG                                 <  SRR4302545.781103/94‑1 (MQ=60)
                                                              CAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACG                                 >  SRR4302545.9523533/1‑94 (MQ=60)
                                                               AGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGG                                >  SRR4302545.1397461/1‑94 (MQ=60)
                                                               AGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGG                                >  SRR4302545.6914320/1‑94 (MQ=60)
                                                               AGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGG                                <  SRR4302545.9747032/94‑1 (MQ=60)
                                                               AGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCAAGG                                >  SRR4302545.770449/1‑94 (MQ=60)
                                                                GCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGT                               >  SRR4302545.2605672/1‑94 (MQ=60)
                                                                GCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGT                               >  SRR4302545.9105981/1‑94 (MQ=60)
                                                                GCTCTTTCAACACTGCGTCAAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGTCCAGAATTGCCTTCACTTGTTCCACGGT                               <  SRR4302545.2331615/94‑1 (MQ=60)
                                                                 CTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACTGTA                              >  SRR4302545.1159327/1‑94 (MQ=60)
                                                                  TCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACCGTAT                             >  SRR4302545.4588422/1‑94 (MQ=60)
                                                                   CTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATG                            >  SRR4302545.7321618/1‑94 (MQ=60)
                                                                   CTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATG                            <  SRR4302545.1812980/94‑1 (MQ=60)
                                                                   CTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATG                            <  SRR4302545.1935772/94‑1 (MQ=60)
                                                                   CTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATG                            >  SRR4302545.10180515/1‑94 (MQ=60)
                                                                    TTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGA                           <  SRR4302545.42663/94‑1 (MQ=60)
                                                                     TTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGAC                          >  SRR4302545.8501297/1‑94 (MQ=60)
                                                                      TCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGACC                         >  SRR4302545.10398763/1‑94 (MQ=60)
                                                                       CAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGACCA                        >  SRR4302545.10520872/1‑94 (MQ=60)
                                                                       CAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGACCA                        <  SRR4302545.10804826/94‑1 (MQ=60)
                                                                       CAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGACCA                        >  SRR4302545.1632552/1‑94 (MQ=60)
                                                                       CAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGACCA                        >  SRR4302545.10154235/1‑94 (MQ=60)
                                                                       CAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGACCA                        <  SRR4302545.5069568/94‑1 (MQ=60)
                                                                        AGTACTGCGTCCAGCACCTTATCTTTCTTCCGGCATTTCACCGGGATGTCGCCTTTGGCCAGAATTGCCTTCACTTGTTCGACGGTATGACCAA                       >  SRR4302545.6648443/1‑94 (MQ=60)
                                                                        AATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCTGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGACCAA                       <  SRR4302545.2983717/94‑1 (MQ=60)
                                                                        AATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGACCAA                       >  SRR4302545.5843967/1‑94 (MQ=60)
                                                                         ATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGACCAAA                      <  SRR4302545.5041288/94‑1 (MQ=60)
                                                                         ATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGCTATGACCACA                      >  SRR4302545.4936810/1‑94 (MQ=60)
                                                                         ATACTGCGCCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGACCAAA                      >  SRR4302545.2993853/1‑94 (MQ=60)
                                                                          TACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGACCAAAA                     >  SRR4302545.1766402/1‑94 (MQ=60)
                                                                          TACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGACCAAAA                     <  SRR4302545.8023584/94‑1 (MQ=60)
                                                                            CTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGACCAAAAAT                   <  SRR4302545.5285036/94‑1 (MQ=60)
                                                                            CTGCGCCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGACCAAAAAT                   <  SRR4302545.4116562/94‑1 (MQ=60)
                                                                             TGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGACCAAAAATA                  >  SRR4302545.2002129/1‑94 (MQ=60)
                                                                             TGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGACCAAAAATA                  <  SRR4302545.10805282/94‑1 (MQ=60)
                                                                              GCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGACCAAAAATAA                 >  SRR4302545.1532741/1‑94 (MQ=60)
                                                                               CGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGACCAAAAATAAA                >  SRR4302545.6082996/1‑94 (MQ=60)
                                                                                GTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGACCAAAAATAAAG               >  SRR4302545.4501279/1‑94 (MQ=60)
                                                                                GTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGACCAAAAATAAAG               >  SRR4302545.5414287/1‑94 (MQ=60)
                                                                                 TCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGACCAAAAATAAAGA              <  SRR4302545.8721783/94‑1 (MQ=60)
                                                                                  CCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGACCAAAAATAAAGAT             >  SRR4302545.827239/1‑94 (MQ=60)
                                                                                  CCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGACCAAAAATAAAGAT             <  SRR4302545.7342846/94‑1 (MQ=60)
                                                                                  CCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGACCAAAAATAAAGAT             >  SRR4302545.8393060/1‑94 (MQ=60)
                                                                                   CAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGACCAAAAATAAAGATA            >  SRR4302545.3260252/1‑94 (MQ=60)
                                                                                   CAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGACCAAAAATAAAGATA            >  SRR4302545.9086711/1‑94 (MQ=60)
                                                                                   CAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGACCAAAAATAAAGATA            <  SRR4302545.8905246/94‑1 (MQ=60)
                                                                                   CAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGACCAAAAATAAAGATA            <  SRR4302545.6551574/94‑1 (MQ=60)
                                                                                   CAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGACCAAAAATAAAGATA            >  SRR4302545.2161748/1‑94 (MQ=60)
                                                                                    AGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGACCAAAAATAAAGATAT           <  SRR4302545.7675805/94‑1 (MQ=60)
                                                                                    AGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGACCAAAAATAAAGATAT           <  SRR4302545.6705894/94‑1 (MQ=60)
                                                                                     GCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGACCAAAAATAAAGATATT          <  SRR4302545.7176494/94‑1 (MQ=60)
                                                                                     GCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGACCAAAAATAAAGATATT          >  SRR4302545.4330546/1‑94 (MQ=60)
                                                                                     GCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGACCAAAAATAAAGATATT          <  SRR4302545.805825/94‑1 (MQ=60)
                                                                                     GCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGACCAAAAATAAAGATATT          <  SRR4302545.2657649/94‑1 (MQ=60)
                                                                                      CACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGACCAAAAATAAAGATATTT         <  SRR4302545.4189611/94‑1 (MQ=60)
                                                                                      CACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGACCAAAAATAAAGATATTT         <  SRR4302545.8975863/94‑1 (MQ=60)
                                                                                      CACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGACCAAAAATAAAGATATTT         >  SRR4302545.3870786/1‑94 (MQ=60)
                                                                                       CCCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGACCAAAAATAAAGATATTTT        <  SRR4302545.2546338/94‑1 (MQ=60)
                                                                                       CCCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGACCAAAAATAAAGATATTTT        <  SRR4302545.7805489/94‑1 (MQ=60)
                                                                                       ACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGACCAAAAATAAAGATATTTT        >  SRR4302545.7412331/1‑94 (MQ=60)
                                                                                       ACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGACCAAAAATAAAGATATTTT        <  SRR4302545.823846/94‑1 (MQ=60)
                                                                                       ACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGACCAAAAATAAAGATATTTT        <  SRR4302545.703651/94‑1 (MQ=60)
                                                                                        CCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGACCAAAAATAAAGATATTTTC       <  SRR4302545.9481010/94‑1 (MQ=60)
                                                                                        CCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGACCAAAAATAAAGATATTTTC       <  SRR4302545.8356273/94‑1 (MQ=60)
                                                                                         CTTATCTTTCTTCCGCCATTTCACCGTGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGACCAAAAATAAAGATATTTTCT      <  SRR4302545.1439098/94‑1 (MQ=60)
                                                                                         CTTATCTTTCTTCCGCCATTTCACCGTGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGACCAAAAATAAAGATATTTTCT      <  SRR4302545.1439097/94‑1 (MQ=60)
                                                                                         CTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGACCAAAAATAAAGATATTTTCT      >  SRR4302545.3734427/1‑94 (MQ=60)
                                                                                         CTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGACCAAAAATAAAGATATTTTCT      >  SRR4302545.9572001/1‑94 (MQ=60)
                                                                                         CTTATCTTTCTTCCGCCATTTCACCGGGGCGTAGCCTTTGGACAGAATTGCCTTCACTTGTTCCACGGTATGACCAAAAATAAAGATATTTTCT      <  SRR4302545.8403656/94‑1 (MQ=60)
                                                                                          TTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGACCAAAAATAAAGATATTTTCTT     <  SRR4302545.831576/94‑1 (MQ=60)
                                                                                            ATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGACCAAAAATAAAGATATTTTCTTCA   >  SRR4302545.8112987/1‑94 (MQ=60)
                                                                                            ATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGACCAAAAATAAAGATATTTTCTTCA   <  SRR4302545.3225906/94‑1 (MQ=60)
                                                                                             TCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGACCAAAAATAAAGATATTTTCTTCAC  >  SRR4302545.3056972/1‑94 (MQ=60)
                                                                                             |                                                                                             
CCGATACTGTGCAGCATCTGGTCGAAGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTACCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGACCAAAAATAAAGATATTTTCTTCAC  >  NC_000913/3547912‑3548098

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑