Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 647 270.1 21258240 98.4% 20918108 94.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,554,118 +C coding (367/396 nt) yhgKJ ← RNA phosphage cyclase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,554,1171.C99.8% 1474.8 / NA 432A123G (GCG→GGG) yhgKJRNA phosphage cyclase
Reads supporting (aligned to +/- strand):  ref base . (0/1);  major base C (213/217);  minor base A (0/1);  total (213/219)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 479 total aligned reads displayed.

GTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCG‑AGGCGGCGCCGACGGGTTATCGGTCGCCG‑CGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGC‑GCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCG  >  NC_000913/3554025‑3554200
                                                                                              |                                                                                    
gTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGt‑gccg‑c                                                                                     >  2:8907932/1‑94 (MQ=255)
 tGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGt‑gccg‑cc                                                                                    >  1:5535319/1‑93 (MQ=255)
 tGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGt‑gccg‑cc                                                                                    >  1:7130795/1‑93 (MQ=255)
 tGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGt‑gccg‑cc                                                                                    <  1:2243226/94‑2 (MQ=255)
 tGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGt‑gccg‑cc                                                                                    <  2:2482338/94‑2 (MQ=255)
      tGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGTCGCCGACGGGTTATCGGT‑GCCGCCGCTCa                                                                                <  1:76598/94‑1 (MQ=255)
      tGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCa                                                                                <  1:3097515/94‑1 (MQ=255)
       gCTGATGACTTCCTATTGTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCAc                                                                               <  1:4120671/94‑1 (MQ=255)
       gCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCAc                                                                               >  1:3309318/1‑94 (MQ=255)
        cTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACt                                                                              >  2:10545650/1‑94 (MQ=255)
         tgatgaATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACtt                                                                             <  2:9213659/94‑1 (MQ=255)
         tgatgaATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACtt                                                                             <  2:4144306/94‑1 (MQ=255)
         tgatgaATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACtt                                                                             <  1:9269486/94‑1 (MQ=255)
         tgatgaATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGGTATCGGT‑GCCGCCGCTCACtt                                                                             >  2:331849/1‑94 (MQ=255)
           atgaATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCa                                                                           >  2:876801/1‑94 (MQ=255)
            tgaATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCaa                                                                          >  1:4897493/1‑94 (MQ=255)
             taaTTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAac                                                                         <  1:10348288/93‑1 (MQ=255)
              aaTTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAaca                                                                        >  1:967126/1‑94 (MQ=255)
              aaTTCCTATTTGCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAaca                                                                        <  1:2935548/94‑1 (MQ=255)
               aTTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAacac                                                                       >  2:8645027/1‑94 (MQ=255)
                ttCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACAcg                                                                      >  2:9448629/1‑94 (MQ=255)
                 tCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACAcgc                                                                     >  1:10090245/1‑94 (MQ=255)
                 tCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACAcgc                                                                     <  2:1286671/94‑1 (MQ=255)
                     tttttCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAg                                                                 <  2:10174008/93‑1 (MQ=255)
                     aTTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCTCTTCCACACGCGAAg                                                                 >  1:6074339/1‑94 (MQ=255)
                     aTTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAg                                                                 <  2:2726899/94‑1 (MQ=255)
                      ttttCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAgg                                                                >  1:1891062/1‑94 (MQ=255)
                       tttCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGACggg                                                               >  2:3901894/1‑93 (MQ=255)
                         tCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTcc                                                             <  1:1417007/94‑1 (MQ=255)
                          cGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCa                                                            >  1:2754193/1‑94 (MQ=255)
                          cGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCa                                                            >  1:319301/1‑94 (MQ=255)
                           gCCATCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCAt                                                           <  2:7417492/94‑1 (MQ=255)
                           gCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCAt                                                           >  2:168509/1‑94 (MQ=255)
                            ccAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATc                                                          <  2:4304192/94‑1 (MQ=255)
                            ccAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATc                                                          <  1:17166/94‑1 (MQ=255)
                             cagcagCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCg                                                         <  1:1556073/94‑1 (MQ=255)
                             cagcagCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCg                                                         <  2:2057901/94‑1 (MQ=255)
                             cagcagCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCg                                                         >  1:7349304/1‑94 (MQ=255)
                              cgcagcGGCTCCAGCACCCGGCGAATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGTTCACTTCAACACGCGAAGGTCCATCgg                                                        <  1:7606550/93‑1 (MQ=255)
                              agcagcGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCgg                                                        >  1:4532481/1‑94 (MQ=255)
                              agcagcGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCgg                                                        >  1:4440293/1‑94 (MQ=255)
                              agcagcGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCgg                                                        >  1:2590394/1‑94 (MQ=255)
                              agcagcGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCgg                                                        >  1:5726892/1‑94 (MQ=255)
                                  gcGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCaaa                                                    >  2:8231654/1‑94 (MQ=255)
                                   tggCTCCAGCACCCGGCGCATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAAc                                                   >  1:5313705/2‑94 (MQ=255)
                                   cGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCCAAc                                                   >  1:8821211/1‑94 (MQ=255)
                                   cGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAAc                                                   <  1:9095445/94‑1 (MQ=255)
                                   cGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAAc                                                   <  2:6807570/94‑1 (MQ=255)
                                    ggCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCACAcc                                                  >  1:1068617/1‑94 (MQ=255)
                                    ggCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAAcc                                                  <  2:5904165/94‑1 (MQ=255)
                                    ggCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAAcc                                                  >  2:6696405/1‑94 (MQ=255)
                                       gccAGAACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACcaca                                               <  2:5794542/93‑1 (MQ=255)
                                         cAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCACACCACAgc                                             >  2:4753196/1‑94 (MQ=255)
                                         cAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAgc                                             >  1:6143290/1‑94 (MQ=255)
                                         cAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAgc                                             <  1:4329248/94‑1 (MQ=255)
                                             aCCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGC‑GCgg                                        >  2:9291484/1‑94 (MQ=255)
                                              cccGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGC‑GCggg                                       <  2:3074453/94‑1 (MQ=255)
                                              cccGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGC‑GCggg                                       <  1:6423714/94‑1 (MQ=255)
                                                cggcggATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGC‑GCGGgca                                     <  2:9620534/94‑1 (MQ=255)
                                                cggcggATAAAACCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGC‑GCGGgca                                     <  2:6352021/94‑1 (MQ=255)
                                                 ggcggATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGC‑GCGGgcag                                    >  2:4586905/1‑94 (MQ=255)
                                                  gcggATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGC‑GCGGgcagc                                   <  2:10023367/94‑1 (MQ=255)
                                                   cggATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGC‑GCGGgcagca                                  >  1:3443021/1‑94 (MQ=255)
                                                    ggATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGC‑GCGGGCAGCAc                                 >  2:3266906/1‑94 (MQ=255)
                                                    ggATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGC‑GCGGGCAGCAc                                 <  2:6107240/94‑1 (MQ=255)
                                                     gATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGC‑GCGGGCAGCAcc                                <  1:3607382/94‑1 (MQ=255)
                                                      aTAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGC‑GCGGGCAGCACCg                               >  2:8379850/1‑94 (MQ=255)
                                                      aTAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGC‑GCGGGCAGCACCg                               >  1:7937893/1‑94 (MQ=255)
                                                      aTAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGC‑GCGGGCAGCACCg                               >  1:3955006/1‑94 (MQ=255)
                                                       tAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGC‑GCGGGCAGCACCGt                              <  2:2951400/94‑1 (MQ=255)
                                                       tAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGC‑GCGGGCAGCACCGt                              >  2:3760391/1‑94 (MQ=255)
                                                       tAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGC‑GCGGGCAGCACCGt                              >  1:6007856/1‑94 (MQ=255)
                                                             ccgcaGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGC‑GCGGGCAGCACCGTTTgcag                        >  1:4750329/5‑94 (MQ=255)
                                                             ccgcaGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGC‑GCGGGCAGCACCGTTTgcag                        <  2:349591/90‑1 (MQ=255)
                                                              cgcaGGCGGCGCCGACGGGTTATCGGT‑GCCGTCGCTCACTTCAACACGCGAAGGTCCATCGGCAAGCCACAGC‑GCGGGCAGCACCGTTTgcagc                       <  2:3601613/91‑1 (MQ=255)
                                                              cgcaGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGC‑GCGGGCAGCACCGTTTgcagc                       <  2:9779635/91‑1 (MQ=255)
                                                               gcaGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGC‑GCGGGCAGCACCGTTTgcagca                      <  2:8537915/92‑1 (MQ=255)
                                                                caGGGGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGC‑GCGGGCAGCACCGTTTGCAGCAc                     <  1:8595008/93‑1 (MQ=255)
                                                                 cggcggcGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGC‑GCGGGCAGCACCGTTTGCAGCAcc                    <  1:2397229/93‑1 (MQ=255)
                                                                   gcggcgCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGC‑GCGGGCAGCACCGTTTGCAGCACCAg                  <  2:5243173/94‑1 (MQ=255)
                                                                   gcggcgCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGC‑GCGGGCAGCACCGTTTGCAGCACCAg                  <  2:779236/94‑1 (MQ=255)
                                                                      gcgcCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGC‑GCGGGCAGCACCGTTTGCAGCACCAGCGt               <  2:6489735/94‑1 (MQ=255)
                                                                        gcCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGC‑GCGGGCAGCACCGTTTGCAGCACCAGCGTAc             <  2:1406540/94‑1 (MQ=255)
                                                                          cGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGACGGTCCATGGGC‑AAGCACAACGGCGGGAAGAACCGTTTGAACAACCAGCGTACac           >  2:3613420/1‑93 (MQ=255)
                                                                           gACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGC‑GCGGGCAGCACCGTTTGCAGCACCAGCGTACAAc          <  2:2293903/94‑1 (MQ=255)
                                                                           gACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGC‑GCGGGCAGCACCGTTTGCAGCACCAGCGTACAAc          >  1:6969631/1‑94 (MQ=255)
                                                                            aCGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGC‑GCGGGCAGCACCGTTTGCAGCACCAGCGTACAACt         <  1:6728040/94‑1 (MQ=255)
                                                                            aCGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGC‑GCGGGCAGCACCGTTTGCAGCACCAGCGTACAACt         <  2:2170407/94‑1 (MQ=255)
                                                                            aCGGGTTATCGG‑AGCCGCCGCTCAATTCAACACGCGAAGGTCCATCGGCAAACCACAGC‑GCGGGCAGCACCGTTTGCAGCACCAGCGTACAACt         >  2:499277/1‑94 (MQ=255)
                                                                             cGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGC‑GCGGGCAGCACCGTTTGCAGCACCAGCGTACAACtt        >  2:7594243/1‑94 (MQ=255)
                                                                              gggTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGC‑GCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTc       <  1:87220/94‑1 (MQ=255)
                                                                               ggTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGC‑GCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTcc      <  2:9027037/94‑1 (MQ=255)
                                                                               ggTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGC‑GCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTcc      <  1:1254363/94‑1 (MQ=255)
                                                                                gTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGC‑GCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCg     <  2:1893852/94‑1 (MQ=255)
                                                                                gTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGC‑GCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCg     <  1:8107985/94‑1 (MQ=255)
                                                                                 ttATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGC‑GCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCgg    <  1:982244/94‑1 (MQ=255)
                                                                                 ttATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGC‑GCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCgg    <  1:3854975/94‑1 (MQ=255)
                                                                                 ttATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGC‑GCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCgg    <  1:2123287/94‑1 (MQ=255)
                                                                                  tATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGC‑GCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGgc   >  2:697611/1‑94 (MQ=255)
                                                                                   aTCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGC‑GCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGgcg  >  2:6237101/1‑94 (MQ=255)
                                                                                              |                                                                                    
GTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCG‑AGGCGGCGCCGACGGGTTATCGGTCGCCG‑CGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGC‑GCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCG  >  NC_000913/3554025‑3554200

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 338 total aligned reads displayed.
TTGATGAGATTGGCGAACTGGGCGCAGACGAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCAGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTCGCGAAGATCTGTACGCGCG  >  NC_000913/3556762‑3556948
                                                                                             |                                                                                             
TTGATGAGATTGGCGAACTGGGCGCAGACGAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCG                                                                                               <  SRR4302545.5328801/94‑1 (MQ=60)
 TGATGAGATTGGCGAACTGGGCGCAGACGAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGG                                                                                              <  SRR4302545.9436625/94‑1 (MQ=60)
  GATGAGATTGGCGAACTGGGCGCAGACGAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGG                                                                                             <  SRR4302545.10018654/94‑1 (MQ=60)
   ATGAGATTGGCGATCGGGGCGCAGACGAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCTGTTTGGCAGCGATCGGCGGGT                                                                                            <  SRR4302545.10146893/94‑1 (MQ=60)
   ATGAGATTGGCGAACTGGGCGCAGACGAACAGGCAATGCTGCTGAAAGCCATTGAAGAGCAAACCTTTTACCCGTTTGGCAGCGATCCCCGGGT                                                                                            >  SRR4302545.8837783/1‑94 (MQ=60)
   ATGAGATTGGCGAACTGGGCGCAGACGAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGT                                                                                            >  SRR4302545.1697981/1‑94 (MQ=60)
   ATGAGATTGGCGAACTGGGCGCAGACGAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGT                                                                                            <  SRR4302545.8703327/94‑1 (MQ=60)
   ATGAGATTGGCGAACTGGGCGCAGACGAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGT                                                                                            <  SRR4302545.2400773/94‑1 (MQ=60)
   ATGAGATTGGCGAACTGGGCGCAGACGAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGT                                                                                            <  SRR4302545.10735101/94‑1 (MQ=60)
   ATGAGATTGGCGAACTGGGCGCAGACGAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGT                                                                                            <  SRR4302545.9614854/94‑1 (MQ=60)
   ATGAGATTGGCGAACTGGGCGCAGACGAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGT                                                                                            <  SRR4302545.3807518/94‑1 (MQ=60)
   ATGAGATTGGCGAACTGGGCGCAGACGAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGT                                                                                            <  SRR4302545.10390211/94‑1 (MQ=60)
   ATGAGATTGGCGAACTGGGCGCAGACGAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGT                                                                                            <  SRR4302545.6127919/94‑1 (MQ=60)
   ATGAGATTGGCGAACTGGGCGCAGACGAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGT                                                                                            <  SRR4302545.9855114/94‑1 (MQ=60)
   ATGAGATTGGCGAACTGGGCGCAGACGAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGT                                                                                            <  SRR4302545.5705943/94‑1 (MQ=60)
    TGAGATTGGCGAACTGGGCGCAGACGAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTG                                                                                           <  SRR4302545.6719471/94‑1 (MQ=60)
    TGAGATTGGCGAACTGGGCGCAGACGAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTG                                                                                           <  SRR4302545.7873797/94‑1 (MQ=60)
     GAGATTGGCGAACTGGGCGCAGACGAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGG                                                                                          >  SRR4302545.7245546/1‑94 (MQ=60)
     GAGATTGGCGAACTGGGCGCAGACGAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGA                                                                                          <  SRR4302545.8133363/94‑1 (MQ=60)
      AGATTGGCGAACTGGGCGCAGACGAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAG                                                                                         >  SRR4302545.2353941/1‑94 (MQ=60)
       GATTGGCGAACTGGGCGCAGACGAACAGGCAATGCTGCTGAAAGCCATTGAAGACAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGC                                                                                        <  SRR4302545.2842112/94‑1 (MQ=60)
         TTGGCGAACTGGGCGCAGACGAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAG                                                                                      >  SRR4302545.4671645/1‑94 (MQ=60)
          TGGCGAACTGGGCGCAGACGAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGT                                                                                     <  SRR4302545.8661945/94‑1 (MQ=60)
          TGGCGAACTGGGCGCAGACGAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGT                                                                                     <  SRR4302545.5518597/94‑1 (MQ=60)
          TGGCGAACTGGGCGCAGACGAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGT                                                                                     <  SRR4302545.9090637/94‑1 (MQ=60)
           GGCGAACTGGGCGCAGACGAACAGGCAATGCTTCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTCGCAGCGATCGCCGGGTGAGGAGTG                                                                                    >  SRR4302545.6839934/1‑94 (MQ=60)
             CGAACTGGTGTCAGACGAACCGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGAT                                                                                  <  SRR4302545.6824018/94‑1 (MQ=60)
             CGAACTGGGCGCAGACGAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGAT                                                                                  >  SRR4302545.3630348/1‑94 (MQ=60)
             CGAACTGGGCGCAGACGAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATAGCCGGGTGAGCAGTGAT                                                                                  >  SRR4302545.4897523/1‑94 (MQ=60)
              GAACTGGGCGCAGACGAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATT                                                                                 >  SRR4302545.237296/1‑94 (MQ=60)
              GAACTGGGCGCAGACGAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATT                                                                                 <  SRR4302545.665405/94‑1 (MQ=60)
              GAACTGGGCGCAGACGAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATT                                                                                 >  SRR4302545.4657225/1‑94 (MQ=60)
              GAACTGGGCGCAGACGAACAGGCAATGCTGCTGAAAGACATTAAAGAGAAAACCTTTTACACGTATGGCAGCGATCGCCGGGTGAGCAGTGACT                                                                                 >  SRR4302545.7339364/1‑94 (MQ=60)
               CACTGGGCGCAGACGAGCAGGCAATGCTGCTGAGCGCCTTTGAAGAGACCACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTT                                                                                <  SRR4302545.9670645/94‑1 (MQ=60)
                ACTGGGCGCAGACGAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTT                                                                               >  SRR4302545.8919714/1‑94 (MQ=60)
                 CTGGGCGCAGACGAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTC                                                                              >  SRR4302545.3591423/1‑94 (MQ=60)
                  TGGGCGCAGACGAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCA                                                                             >  SRR4302545.3214160/1‑94 (MQ=60)
                   GGGCGCAGACGAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAG                                                                            >  SRR4302545.502211/1‑94 (MQ=60)
                   GGGCGCAGACGAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAG                                                                            <  SRR4302545.3651319/94‑1 (MQ=60)
                   GGGCGCAGACGAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGGGAGCAGTGATTTTCAG                                                                            >  SRR4302545.8577904/1‑94 (MQ=60)
                   GGGCGCAGACGAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCAGATTGGCAGCGACTGCCGGGTGAGCAGTGATTTTCTG                                                                            >  SRR4302545.7161997/1‑94 (MQ=60)
                    GGCGCAGACGAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGC                                                                           <  SRR4302545.2507332/94‑1 (MQ=60)
                     GCGCAGACGAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCT                                                                          <  SRR4302545.9580391/94‑1 (MQ=60)
                       GCAGACGAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTA                                                                        <  SRR4302545.6562970/94‑1 (MQ=60)
                       GCAGACGAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTA                                                                        >  SRR4302545.6148854/1‑94 (MQ=60)
                       GCAGACGAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTA                                                                        >  SRR4302545.6148855/1‑94 (MQ=60)
                        CAGACGAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTAT                                                                       <  SRR4302545.9574154/94‑1 (MQ=60)
                        CAGACGAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTAT                                                                       <  SRR4302545.5285995/94‑1 (MQ=60)
                        CAGACGAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTAT                                                                       >  SRR4302545.1059217/1‑94 (MQ=60)
                        CAGACGAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTAT                                                                       <  SRR4302545.960709/94‑1 (MQ=60)
                         AGACGAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATC                                                                      >  SRR4302545.6284361/1‑94 (MQ=60)
                         AGACGAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATC                                                                      >  SRR4302545.1478480/1‑94 (MQ=60)
                         AGACGAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATC                                                                      >  SRR4302545.2651511/1‑94 (MQ=60)
                         AGACGAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATC                                                                      <  SRR4302545.4808085/94‑1 (MQ=60)
                         AGACGAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATATTCAGCTTATC                                                                      >  SRR4302545.2486261/1‑94 (MQ=60)
                          GACGAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCG                                                                     <  SRR4302545.4661239/94‑1 (MQ=60)
                          GACGAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCG                                                                     >  SRR4302545.3268303/1‑94 (MQ=60)
                           ACGAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGC                                                                    >  SRR4302545.172009/1‑94 (MQ=60)
                            CGAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCC                                                                   <  SRR4302545.7380663/94‑1 (MQ=60)
                            CGAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCC                                                                   >  SRR4302545.8442590/1‑94 (MQ=60)
                             GAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCG                                                                  >  SRR4302545.10084552/1‑94 (MQ=60)
                               ACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGA                                                                >  SRR4302545.8753140/1‑94 (MQ=60)
                               ACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCCCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGA                                                                >  SRR4302545.2384169/1‑94 (MQ=60)
                                CAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAA                                                               <  SRR4302545.8547202/94‑1 (MQ=60)
                                 AGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAAC                                                              <  SRR4302545.5142550/94‑1 (MQ=60)
                                 AGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAAC                                                              <  SRR4302545.2432715/94‑1 (MQ=60)
                                 AGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAAC                                                              <  SRR4302545.8966440/94‑1 (MQ=60)
                                    CAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGG                                                           >  SRR4302545.1576982/1‑94 (MQ=60)
                                     AATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTG                                                          >  SRR4302545.6093885/1‑94 (MQ=60)
                                     AATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTG                                                          >  SRR4302545.3217320/1‑94 (MQ=60)
                                     AATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTG                                                          >  SRR4302545.3226065/1‑94 (MQ=60)
                                     AATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTG                                                          <  SRR4302545.1247660/94‑1 (MQ=60)
                                     AATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTG                                                          <  SRR4302545.9001770/94‑1 (MQ=60)
                                     AATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTG                                                          >  SRR4302545.8024889/1‑94 (MQ=60)
                                      ATGCTGCTGAGAGCCATTGAAGAGATAACCGTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGC                                                         <  SRR4302545.1899354/94‑1 (MQ=60)
                                      ATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGC                                                         >  SRR4302545.3483132/1‑94 (MQ=60)
                                      ATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGC                                                         >  SRR4302545.2876847/1‑94 (MQ=60)
                                      ATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTATCAGCTTATCGCCGGAACGGTGC                                                         >  SRR4302545.5987772/1‑94 (MQ=60)
                                       TGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCG                                                        >  SRR4302545.7996091/1‑94 (MQ=60)
                                       AGCTGCTGAAAACCACTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCG                                                        <  SRR4302545.8518213/94‑1 (MQ=60)
                                         CTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCG                                                      >  SRR4302545.6542304/1‑94 (MQ=60)
                                         CTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCG                                                      <  SRR4302545.10521240/94‑1 (MQ=60)
                                         CTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCG                                                      <  SRR4302545.568876/94‑1 (MQ=60)
                                          TGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGA                                                     >  SRR4302545.9352011/1‑94 (MQ=60)
                                          TGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGA                                                     >  SRR4302545.2270774/1‑94 (MQ=60)
                                           GCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGAT                                                    >  SRR4302545.5842298/1‑94 (MQ=60)
                                           GCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGAT                                                    >  SRR4302545.10152035/1‑94 (MQ=60)
                                           GCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGAT                                                    >  SRR4302545.1476917/1‑94 (MQ=60)
                                            CTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATT                                                   >  SRR4302545.8734486/1‑94 (MQ=60)
                                            CTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATT                                                   >  SRR4302545.10282005/1‑94 (MQ=60)
                                            CTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATT                                                   >  SRR4302545.3766070/1‑94 (MQ=60)
                                             TGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTT                                                  <  SRR4302545.8997159/94‑1 (MQ=60)
                                             TGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTT                                                  >  SRR4302545.4720847/1‑94 (MQ=60)
                                             TGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTT                                                  >  SRR4302545.82771/1‑94 (MQ=60)
                                             TGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTT                                                  >  SRR4302545.1380567/1‑94 (MQ=60)
                                              GAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTG                                                 <  SRR4302545.4469510/94‑1 (MQ=60)
                                               AAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGC                                                >  SRR4302545.10814489/1‑94 (MQ=60)
                                               AAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGC                                                >  SRR4302545.2073044/1‑94 (MQ=60)
                                               AAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGGGCGCGATTTGC                                                >  SRR4302545.7869864/1‑94 (MQ=60)
                                                AAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCG                                               >  SRR4302545.5759699/1‑94 (MQ=60)
                                                AAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCG                                               >  SRR4302545.10023400/1‑94 (MQ=60)
                                                AAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCG                                               >  SRR4302545.3574423/1‑94 (MQ=60)
                                                AAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCG                                               <  SRR4302545.4964358/94‑1 (MQ=60)
                                                 AGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCCGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGAGC                                              >  SRR4302545.8536391/1‑94 (MQ=60)
                                                  GCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCC                                             <  SRR4302545.2627910/94‑1 (MQ=60)
                                                   CCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCA                                            >  SRR4302545.3486189/1‑94 (MQ=60)
                                                   CCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCA                                            >  SRR4302545.2754322/1‑94 (MQ=60)
                                                   CCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCA                                            >  SRR4302545.8526654/1‑94 (MQ=60)
                                                    CATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAG                                           >  SRR4302545.5557837/1‑94 (MQ=60)
                                                    CATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAG                                           >  SRR4302545.10885713/1‑94 (MQ=60)
                                                    CATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAG                                           >  SRR4302545.5282525/1‑94 (MQ=60)
                                                     ATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGC                                          >  SRR4302545.7401791/1‑94 (MQ=60)
                                                     ATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGC                                          >  SRR4302545.2700159/1‑94 (MQ=60)
                                                     ATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGC                                          >  SRR4302545.210350/1‑94 (MQ=60)
                                                      TTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCT                                         >  SRR4302545.3668624/1‑94 (MQ=60)
                                                      TTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCT                                         <  SRR4302545.8801688/94‑1 (MQ=60)
                                                      TTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCT                                         >  SRR4302545.8075356/1‑94 (MQ=60)
                                                       TGAAGAGAAAACCTTTTACGCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGATTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTG                                        <  SRR4302545.6807843/94‑1 (MQ=60)
                                                        GAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGG                                       <  SRR4302545.8540322/94‑1 (MQ=60)
                                                         AAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGT                                      >  SRR4302545.6960024/1‑94 (MQ=60)
                                                         AAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGT                                      <  SRR4302545.2041582/94‑1 (MQ=60)
                                                         AAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGCGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGT                                      >  SRR4302545.6293409/1‑94 (MQ=60)
                                                          AGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTT                                     >  SRR4302545.8190146/1‑94 (MQ=60)
                                                          AGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTT                                     >  SRR4302545.4192598/1‑94 (MQ=60)
                                                          AGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTT                                     >  SRR4302545.6802652/1‑94 (MQ=60)
                                                          AGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTT                                     <  SRR4302545.8907275/94‑1 (MQ=60)
                                                           GAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTG                                    >  SRR4302545.173340/1‑94 (MQ=60)
                                                           GAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTG                                    >  SRR4302545.7784713/1‑94 (MQ=60)
                                                            AGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGC                                   <  SRR4302545.2787919/94‑1 (MQ=60)
                                                            AGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGC                                   >  SRR4302545.4625311/1‑94 (MQ=60)
                                                            AGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGC                                   <  SRR4302545.9533893/94‑1 (MQ=60)
                                                             GAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCC                                  >  SRR4302545.276347/1‑94 (MQ=60)
                                                             GAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCC                                  >  SRR4302545.5057657/1‑94 (MQ=60)
                                                              AAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCG                                 <  SRR4302545.10629745/94‑1 (MQ=60)
                                                               AAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGA                                >  SRR4302545.6992227/1‑94 (MQ=60)
                                                               AAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGA                                >  SRR4302545.4097605/1‑94 (MQ=60)
                                                               AAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGA                                <  SRR4302545.7547538/94‑1 (MQ=60)
                                                               AAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGA                                <  SRR4302545.5896229/94‑1 (MQ=60)
                                                                 ACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAG                              >  SRR4302545.6641665/1‑94 (MQ=60)
                                                                 ACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAG                              >  SRR4302545.7563356/1‑94 (MQ=60)
                                                                 ACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAG                              >  SRR4302545.5531490/1‑94 (MQ=60)
                                                                  CCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGG                             <  SRR4302545.2208759/94‑1 (MQ=60)
                                                                   CTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGGCGAAGGC                            >  SRR4302545.1518549/1‑94 (MQ=60)
                                                                   CTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGC                            >  SRR4302545.4187525/1‑94 (MQ=60)
                                                                   CTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGC                            <  SRR4302545.6201228/94‑1 (MQ=60)
                                                                   CTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGC                            >  SRR4302545.1518550/1‑94 (MQ=60)
                                                                    TTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCA                           >  SRR4302545.8436550/1‑94 (MQ=60)
                                                                     TTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAA                          <  SRR4302545.5973787/94‑1 (MQ=60)
                                                                      TTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAA                         >  SRR4302545.2081981/1‑94 (MQ=60)
                                                                       TACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGCAGGCAAAT                        >  SRR4302545.7908256/1‑94 (MQ=60)
                                                                       TACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAAT                        >  SRR4302545.8052417/1‑94 (MQ=60)
                                                                       TACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAAT                        >  SRR4302545.9252504/1‑94 (MQ=60)
                                                                       TACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAAT                        >  SRR4302545.4815474/1‑94 (MQ=60)
                                                                       TACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAAT                        <  SRR4302545.6996491/94‑1 (MQ=60)
                                                                        TCCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATT                       <  SRR4302545.8554301/94‑1 (MQ=60)
                                                                        ACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATT                       <  SRR4302545.1073739/94‑1 (MQ=60)
                                                                        ACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATT                       >  SRR4302545.177122/1‑94 (MQ=60)
                                                                         CCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTT                      >  SRR4302545.1953194/1‑94 (MQ=60)
                                                                         CCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTT                      >  SRR4302545.4481735/1‑94 (MQ=60)
                                                                          CCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTC                     >  SRR4302545.3520793/1‑94 (MQ=60)
                                                                          CCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTC                     >  SRR4302545.5835451/1‑94 (MQ=60)
                                                                          CCGTTTGGCAGCGATCGCCGAGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTC                     <  SRR4302545.4267661/94‑1 (MQ=60)
                                                                           CGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTCG                    <  SRR4302545.10922692/94‑1 (MQ=60)
                                                                            atttggctgttATCGCCGGGTGAGCATTGATTTTTAGCTTATCGCCCGAACCGTCCGCGATTTGCGCCAGCTGGCTGCCGAATTCCAATTTCGC                   >  SRR4302545.9928527/12‑94 (MQ=29)
                                                                            GTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTCGC                   <  SRR4302545.1210653/94‑1 (MQ=60)
                                                                             TTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTCGCG                  <  SRR4302545.6074355/94‑1 (MQ=60)
                                                                              TTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTCGCGA                 >  SRR4302545.1088692/1‑94 (MQ=60)
                                                                               TGGCAGCGATCGTCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTCGCGAA                >  SRR4302545.10758302/1‑94 (MQ=60)
                                                                               TGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTCGCGAA                >  SRR4302545.4616403/1‑94 (MQ=60)
                                                                               TGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTCGCGAA                <  SRR4302545.6251825/94‑1 (MQ=60)
                                                                               TGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTCGCGAA                >  SRR4302545.7091292/1‑94 (MQ=60)
                                                                               TGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTCGCGAA                >  SRR4302545.2543319/1‑94 (MQ=60)
                                                                               TGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTGGCCGAAGGCAAATTGCGCGAA                >  SRR4302545.537404/1‑94 (MQ=60)
                                                                                GGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTCGCGAAG               >  SRR4302545.3708524/1‑94 (MQ=60)
                                                                                 GCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTCGCGAAGA              <  SRR4302545.10310432/94‑1 (MQ=60)
                                                                                  CAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTCGCGAAGAT             <  SRR4302545.7430480/94‑1 (MQ=60)
                                                                                  CAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTCGCGAAGAT             >  SRR4302545.7198107/1‑94 (MQ=60)
                                                                                  CAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCAGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTCGCGAAGAT             >  SRR4302545.9220382/1‑94 (MQ=60)
                                                                                   AGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGACGATGGCAAATTTCcccaggaac            >  SRR4302545.8398332/1‑85 (MQ=60)
                                                                                    GCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTCGCGAAGATCT           <  SRR4302545.4188006/94‑1 (MQ=60)
                                                                                    GCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTCGCGAAGATCT           <  SRR4302545.1180603/94‑1 (MQ=60)
                                                                                    GCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTCGCGAAGATCT           <  SRR4302545.6013381/94‑1 (MQ=60)
                                                                                    GCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTCGCGAAGATCT           >  SRR4302545.8275736/1‑94 (MQ=60)
                                                                                     CGATCGCCAGGGGAGCAGGGATTTTCAGCTTATTGCCGGAACGGGGCGCGATTTGCGCCAGCTGGTTGCCGAAGGTAATTTTCGCGAAGATCTG          >  SRR4302545.7587080/1‑94 (MQ=60)
                                                                                      GATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTCGCGAAGATCTGT         >  SRR4302545.8933234/1‑94 (MQ=60)
                                                                                       ATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTCGCGAAGATCTGTA        <  SRR4302545.9103477/94‑1 (MQ=60)
                                                                                        TCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTCGCGAAGATCTGTAC       <  SRR4302545.708801/94‑1 (MQ=60)
                                                                                        TCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTCGCGAAGATCTGTAC       >  SRR4302545.10231711/1‑94 (MQ=60)
                                                                                         CGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTCGCGAAGATCTGTACG      >  SRR4302545.3890384/1‑94 (MQ=60)
                                                                                         CGCCGGGTGAGCAGTGATTTTCAGCTTATCGCAGGAACAGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGTGAAATTTCGCGAAGATATGTACG      >  SRR4302545.9371523/1‑94 (MQ=60)
                                                                                          GCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTCGCGAAGATCTGTACGC     >  SRR4302545.6141269/1‑94 (MQ=60)
                                                                                          GCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTCGCGAAGATCTGTACGC     <  SRR4302545.5088048/94‑1 (MQ=60)
                                                                                          GCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTCGCGAAGATCTGTACGC     <  SRR4302545.9825260/94‑1 (MQ=60)
                                                                                          GCCGGGTGAGCAGTGATTTTCAGCTTATCGCAGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTCGCGAAGATCTGTACGC     <  SRR4302545.4609742/94‑1 (MQ=60)
                                                                                           CCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTCGCGAAGATCTGTACGCG    >  SRR4302545.8911396/1‑94 (MQ=60)
                                                                                           CCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTCGCGAAGATCTGTACGCG    >  SRR4302545.3009273/1‑94 (MQ=60)
                                                                                           CCGGGTGAGCAGTGATTTTCAACATATCGCCGGAACGGTGCGCGAATTGTGCCAGCTGGTAGCCGAACGGAAATTTCGCGAAGATCTGTACGCG    >  SRR4302545.497171/1‑94 (MQ=60)
                                                                                            CGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTCGCGAAGATCTGTACGCGC   >  SRR4302545.9833284/1‑94 (MQ=60)
                                                                                             GGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTCGCGAAGATCTGTACGCGCG  >  SRR4302545.6721317/1‑94 (MQ=60)
                                                                                             GGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTCGCGAAGATCTGTACGCGCG  <  SRR4302545.7994794/94‑1 (MQ=60)
                                                                                             |                                                                                             
TTGATGAGATTGGCGAACTGGGCGCAGACGAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCAGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTCGCGAAGATCTGTACGCGCG  >  NC_000913/3556762‑3556948

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑