Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,556,855 A→G Q319R (CAG→CGG)  rtcR → putative 2‑component regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,556,8550AG100.0% 877.7 / NA 251Q319R (CAG→CGG) rtcRputative 2‑component regulator
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (132/119);  total (132/119)

Only 100 of 259 total aligned reads displayed.

TGAGATTGGCGAACTGGGCGCAGACGAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCAGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTCGCGAAGATCTGTACG  >  NC_000913/3556766‑3556944
                                                                                         |                                                                                         
tgaGATTGGCGAACTGGGCGCAGACGAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTAACCGTTTGGCAGCGATCGCCGGGt                                                                                        >  1:1570391/1‑93 (MQ=255)
  agaTTGGCGAACTGGGCGCAGACGAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGa                                                                                      <  1:2120377/93‑1 (MQ=255)
     ttGGCGAACTGGGCGCAGACGAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCa                                                                                   >  2:1090618/1‑93 (MQ=255)
      tGGCGAACTGGGCGCAGACGAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAg                                                                                  <  2:4492649/93‑1 (MQ=255)
          gAACTGGGCGCAGACGAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGAt                                                                              <  1:5103789/93‑1 (MQ=255)
           aaCTGGGCGCAGACGAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGAtt                                                                             >  1:1051728/1‑93 (MQ=255)
           aaCTGGGCGCAGACGAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGAtt                                                                             <  1:1306394/93‑1 (MQ=255)
              tGGGCGCAGACGAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTc                                                                          >  1:3669203/1‑93 (MQ=255)
              tGGGCGCAGACGAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTc                                                                          >  1:53011/1‑93 (MQ=255)
                  cgcAGACGAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCt                                                                      >  2:6357000/1‑93 (MQ=255)
                    cAGACGAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTa                                                                    <  2:5307989/93‑1 (MQ=255)
                    cAGACGAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTa                                                                    <  1:2998913/93‑1 (MQ=255)
                     aGACGAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTAt                                                                   <  1:766230/93‑1 (MQ=255)
                     aGACGAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTAt                                                                   >  1:2639600/1‑93 (MQ=255)
                      gACGAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTTAGCAGTGATTTTCAGCTTATc                                                                  <  2:5241087/93‑1 (MQ=255)
                      gACGAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATc                                                                  >  2:3492112/1‑93 (MQ=255)
                         gAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGcc                                                               >  2:2398921/1‑93 (MQ=255)
                         gAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGcc                                                               <  1:5874960/93‑1 (MQ=255)
                          aaCAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCg                                                              <  1:1658174/93‑1 (MQ=255)
                          aaCAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACATTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCg                                                              >  1:6403101/1‑93 (MQ=255)
                           aCAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCgg                                                             >  1:4587254/1‑93 (MQ=255)
                            cAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGa                                                            >  1:330280/1‑93 (MQ=255)
                            cAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGa                                                            >  2:1579458/1‑93 (MQ=255)
                            cAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGa                                                            >  2:5000179/1‑93 (MQ=255)
                            cAGACAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGa                                                            <  1:1823025/93‑1 (MQ=255)
                             aGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGTCGGaa                                                           <  2:4205737/93‑1 (MQ=255)
                             aGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGaa                                                           >  1:1434293/1‑93 (MQ=255)
                              ggCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAAc                                                          <  2:2241521/93‑1 (MQ=255)
                              ggCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAAc                                                          <  2:4610743/93‑1 (MQ=255)
                              ggCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAAc                                                          <  2:5442924/93‑1 (MQ=255)
                                cAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACgg                                                        <  1:2277547/93‑1 (MQ=255)
                                cAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACgg                                                        <  1:5665433/93‑1 (MQ=255)
                                  aTGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTg                                                      <  2:4028660/93‑1 (MQ=255)
                                   tgctgcTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTgc                                                     <  2:1733609/93‑1 (MQ=255)
                                     ctgctgAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCTGGAACGGTgcgc                                                   <  2:3402955/93‑1 (MQ=255)
                                     ctgctgAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTgcgc                                                   <  2:3909964/93‑1 (MQ=255)
                                       gctgAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGa                                                 >  1:2413745/1‑93 (MQ=255)
                                        ctgAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGAt                                                >  2:6383855/1‑93 (MQ=255)
                                         tgAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGAtt                                               <  2:2813104/93‑1 (MQ=255)
                                         tgAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGAtt                                               <  1:6253290/93‑1 (MQ=255)
                                          gAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGTACGGTGCGCGAttt                                              >  2:2336274/1‑93 (MQ=255)
                                          gAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGAttt                                              <  2:4818685/93‑1 (MQ=255)
                                           aaaGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTg                                             >  1:5334837/1‑93 (MQ=255)
                                            aaGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTgc                                            <  1:904994/93‑1 (MQ=255)
                                            aaGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGCGCGCGATTTgc                                            >  2:3295453/1‑93 (MQ=255)
                                              gCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTgcgc                                          >  2:1381409/1‑93 (MQ=255)
                                               ccATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGcc                                         >  2:6343005/1‑93 (MQ=255)
                                                  ttGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGc                                      <  1:4250983/93‑1 (MQ=255)
                                                  ttGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGc                                      >  2:3362735/1‑93 (MQ=255)
                                                  ttGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGc                                      <  1:4988473/93‑1 (MQ=255)
                                                   tGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCt                                     <  2:6160338/93‑1 (MQ=255)
                                                    gAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTg                                    >  2:5747728/1‑93 (MQ=255)
                                                    gAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTg                                    >  2:5094918/1‑93 (MQ=255)
                                                      agagAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGt                                  >  2:3641375/1‑93 (MQ=255)
                                                       gagaAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGtt                                 >  2:5924712/1‑93 (MQ=255)
                                                       gagaAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGtt                                 <  2:4555311/93‑1 (MQ=255)
                                                       gagaAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGtt                                 >  2:910795/1‑93 (MQ=255)
                                                         gaAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGc                               >  1:2533625/1‑93 (MQ=255)
                                                         gaAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGc                               >  1:5752326/1‑93 (MQ=255)
                                                         gaAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGc                               >  2:653070/1‑93 (MQ=255)
                                                          aaaaCCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGcc                              >  2:1249046/1‑93 (MQ=255)
                                                           aaaCCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCg                             >  1:4772627/1‑93 (MQ=255)
                                                           aaaCCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCg                             >  2:5222533/1‑93 (MQ=255)
                                                            aaCCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGa                            <  2:2047405/93‑1 (MQ=255)
                                                            aaCCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGa                            >  2:2527053/1‑93 (MQ=255)
                                                             aCCTTTTACCCGTTTGTCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGaa                           <  2:3181604/93‑1 (MQ=255)
                                                               cTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAgg                         >  1:3442055/1‑93 (MQ=255)
                                                                ttttACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAgga                        >  1:2806787/1‑92 (MQ=255)
                                                                ttttACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGc                        >  2:3461501/1‑93 (MQ=255)
                                                                ttttACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGc                        >  1:2069922/1‑93 (MQ=255)
                                                                ttttACCCGTTTGGCACCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAgga                        >  2:1867549/1‑92 (MQ=255)
                                                                 tttACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCa                       >  1:1341120/1‑93 (MQ=255)
                                                                  ttACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCaa                      <  2:308045/93‑1 (MQ=255)
                                                                   tACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCaaa                     >  2:5577927/1‑93 (MQ=255)
                                                                    aCCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAAt                    <  2:5974936/93‑1 (MQ=255)
                                                                    aCCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAAt                    >  2:4755056/1‑93 (MQ=255)
                                                                    aCCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAAt                    >  1:3175551/1‑93 (MQ=255)
                                                                     cccGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAAtt                   <  1:3041308/93‑1 (MQ=255)
                                                                      ccGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAAttt                  >  1:5437736/1‑93 (MQ=255)
                                                                      ccGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAAttt                  >  1:3961562/1‑93 (MQ=255)
                                                                      ccGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGCGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAAttt                  >  1:1104716/1‑93 (MQ=255)
                                                                       cGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTc                 >  1:310944/1‑93 (MQ=255)
                                                                        gTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTcg                <  1:1932747/93‑1 (MQ=255)
                                                                            ggCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCTGAAGGCAAATTTCGCGaa            <  1:6128232/93‑1 (MQ=255)
                                                                            ggCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTCGCGaa            <  1:4224879/93‑1 (MQ=255)
                                                                             gCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTCGCGAAg           >  1:6457044/1‑93 (MQ=255)
                                                                              cAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCAAAGGCAAATTTCGCGAAGa          <  2:3678148/93‑1 (MQ=255)
                                                                               aGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAGCGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTCGCGAAGAt         >  1:4558378/1‑93 (MQ=255)
                                                                               aGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTCGCGAAGAt         >  2:1991925/1‑93 (MQ=255)
                                                                                gCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTCGCGAAGATc        <  2:763677/93‑1 (MQ=255)
                                                                                gCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTCGCGAAGATc        >  2:2752957/1‑93 (MQ=255)
                                                                                gCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCAAGCTGGTTGCCGAAGGCAAATTTCGCGAAGATc        <  1:4368779/93‑1 (MQ=255)
                                                                                 cGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTCGCGAAGATCt       <  2:6100722/93‑1 (MQ=255)
                                                                                 cGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTCGCGAAGATCt       <  2:2957313/93‑1 (MQ=255)
                                                                                  gATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTCGCGAAGATCTg      <  1:5060189/93‑1 (MQ=255)
                                                                                   aTCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTCGCGAAGATCCGt     <  1:3560330/93‑1 (MQ=255)
                                                                                    tCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTCGCGAAGATCTGTa    >  2:4266940/1‑93 (MQ=255)
                                                                                    tCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTCGCGAAGATCTGTa    <  1:5191266/93‑1 (MQ=255)
                                                                                     cGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTCGCGAAGATCTGTAc   <  1:3710667/93‑1 (MQ=255)
                                                                                     cGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTCGCGAAGATCTGTAc   <  2:3783313/93‑1 (MQ=255)
                                                                                         |                                                                                         
TGAGATTGGCGAACTGGGCGCAGACGAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCAGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTCGCGAAGATCTGTACG  >  NC_000913/3556766‑3556944

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGATGAGATTGGCGAACTGGGCGCAGACGAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCAGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTCGCGAAGATCTGTACGCGCG  >  NC_000913/3556763‑3556948
                                                                                            |                                                                                             
TGATGAGATTGGCGAACTGGGCGCAGACGAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGG                                                                                              >  SRR4302537.274326/1‑94 (MQ=60)
  ATGAGATTGGCGAACTGGGCGCAGACGAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGT                                                                                            >  SRR4302537.3042248/1‑94 (MQ=60)
  ATGAGATTGGCGAACTGGGCGCAGACGAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTAACCGTTTGGCAGCGATCGCCGGGT                                                                                            >  SRR4302537.1581348/1‑94 (MQ=60)
    GAGATTGGCGAACTGGGCGCAGACGAACAGGCAATGCTGCTGAAATCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGA                                                                                          >  SRR4302537.2302939/1‑94 (MQ=60)
    GAGATTGGCGAACTGGGCGCAGACGAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGA                                                                                          <  SRR4302537.3015060/94‑1 (MQ=60)
    GAGATTGGCGAACTGGGCGCAGACGAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGA                                                                                          >  SRR4302537.306107/1‑94 (MQ=60)
     AGATTGGCGAACTGGGCGCAGACGAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAG                                                                                         <  SRR4302537.2135299/94‑1 (MQ=60)
     AGATTGGCGAACTGGGCGCAGACGAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAG                                                                                         <  SRR4302537.1528450/94‑1 (MQ=60)
       ATTGGCGAACTGGGCGCAGACGAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCA                                                                                       <  SRR4302537.5025354/94‑1 (MQ=60)
         TGGCGAACTGGGCGCAGACGAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGT                                                                                     >  SRR4302537.4676612/1‑94 (MQ=60)
          GGCGAACTGGGCGCAGACGAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTG                                                                                    >  SRR4302537.1861008/1‑94 (MQ=60)
          GGCGAACTGGGCGCAGACGAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTG                                                                                    <  SRR4302537.5641429/94‑1 (MQ=60)
             GAACTGGGCGCAGACGAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATT                                                                                 <  SRR4302537.5543180/94‑1 (MQ=60)
             GAACTGGGCGCAGACGAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATT                                                                                 <  SRR4302537.5138962/94‑1 (MQ=60)
             GAACTGGGCGCAGACGAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATT                                                                                 >  SRR4302537.2340979/1‑94 (MQ=60)
             GAACTGGGCGCAGACGAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATT                                                                                 >  SRR4302537.4499501/1‑94 (MQ=60)
             GAACTGGGCGCAGACGAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATT                                                                                 >  SRR4302537.1058982/1‑94 (MQ=60)
              AACTGGGCGCAGACGAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTT                                                                                >  SRR4302537.1366344/1‑94 (MQ=60)
              AACTGGGCGCAGACGAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTT                                                                                <  SRR4302537.1315489/94‑1 (MQ=60)
               ACTGGGCGCAGACGAACATGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTT                                                                               <  SRR4302537.4882583/94‑1 (MQ=60)
               ACTGGGCGCAGACGAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTT                                                                               >  SRR4302537.1811408/1‑94 (MQ=60)
                CTGGGCGCAGACGAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTC                                                                              >  SRR4302537.3364712/1‑94 (MQ=60)
                CTGGGCGCAGACGAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTC                                                                              >  SRR4302537.53401/1‑94 (MQ=60)
                CTGGGCGCAGACGAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTC                                                                              >  SRR4302537.3695021/1‑94 (MQ=60)
                 TGGGCGCAGACGAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAAGCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCA                                                                             >  SRR4302537.1288416/1‑94 (MQ=60)
                 TGGGCGCAGACGAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCA                                                                             <  SRR4302537.4659882/94‑1 (MQ=60)
                 TGGGCGCAGACGAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCA                                                                             <  SRR4302537.1668827/94‑1 (MQ=60)
                  GGGCGCAGACGAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAG                                                                            <  SRR4302537.4594852/94‑1 (MQ=60)
                    GCGCAGACGAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCT                                                                          <  SRR4302537.5121829/94‑1 (MQ=60)
                     CGCAGACGAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTT                                                                         >  SRR4302537.4302508/1‑94 (MQ=60)
                      GCAGACGAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCGGCTTA                                                                        >  SRR4302537.1659/1‑94 (MQ=60)
                      GCAGACGAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTA                                                                        >  SRR4302537.6064884/1‑94 (MQ=60)
                       CAGACGAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTAT                                                                       >  SRR4302537.2658070/1‑94 (MQ=60)
                       CAGACGAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTAT                                                                       <  SRR4302537.2212134/94‑1 (MQ=60)
                       CAGACGAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTAT                                                                       <  SRR4302537.3019819/94‑1 (MQ=60)
                        AGACGAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATC                                                                      <  SRR4302537.2726100/94‑1 (MQ=60)
                        AGACGAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATC                                                                      <  SRR4302537.771477/94‑1 (MQ=60)
                         GACGAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCG                                                                     <  SRR4302537.6478632/94‑1 (MQ=60)
                            GAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCG                                                                  <  SRR4302537.5915264/94‑1 (MQ=60)
                            GAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCG                                                                  <  SRR4302537.4033235/94‑1 (MQ=60)
                            GAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGGGAGCAGTGATTTTCAGCTTATCGCCG                                                                  >  SRR4302537.1173097/1‑94 (MQ=60)
                            GAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACATTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCG                                                                  >  SRR4302537.6446685/1‑94 (MQ=60)
                             AACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGG                                                                 <  SRR4302537.1669795/94‑1 (MQ=60)
                             AACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGG                                                                 >  SRR4302537.4618982/1‑94 (MQ=60)
                              ACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGA                                                                >  SRR4302537.419372/1‑94 (MQ=60)
                              ACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGA                                                                >  SRR4302537.332573/1‑94 (MQ=60)
                               CAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAA                                                               >  SRR4302537.1444269/1‑94 (MQ=60)
                               CAGACAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAA                                                               <  SRR4302537.1835732/94‑1 (MQ=60)
                                AGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAAC                                                              <  SRR4302537.877630/94‑1 (MQ=60)
                                AGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAAC                                                              >  SRR4302537.3078045/1‑94 (MQ=60)
                                  GCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGG                                                            <  SRR4302537.330906/94‑1 (MQ=60)
                                   CAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGT                                                           <  SRR4302537.2293625/94‑1 (MQ=60)
                                   CAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGT                                                           <  SRR4302537.530794/94‑1 (MQ=60)
                                   CAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGT                                                           <  SRR4302537.5704194/94‑1 (MQ=60)
                                    AATGCTGCTGAAAGCCATTGAAGAGAAACCCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTG                                                          <  SRR4302537.5334304/94‑1 (MQ=60)
                                     ATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGC                                                         >  SRR4302537.538328/1‑94 (MQ=60)
                                      TGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCG                                                        >  SRR4302537.3661883/1‑94 (MQ=60)
                                      TGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCG                                                        <  SRR4302537.5042402/94‑1 (MQ=60)
                                         TGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGA                                                     >  SRR4302537.2430731/1‑94 (MQ=60)
                                          GCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGAT                                                    <  SRR4302537.5756957/94‑1 (MQ=60)
                                          GCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGAT                                                    >  SRR4302537.689190/1‑94 (MQ=60)
                                            TGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTT                                                  <  SRR4302537.6296250/94‑1 (MQ=60)
                                             GAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTG                                                 >  SRR4302537.4423648/1‑94 (MQ=60)
                                             GAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTG                                                 >  SRR4302537.5371418/1‑94 (MQ=60)
                                             GAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTG                                                 >  SRR4302537.4308800/1‑94 (MQ=60)
                                              AAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGGGGGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGC                                                >  SRR4302537.5344486/1‑94 (MQ=60)
                                               AAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCG                                               <  SRR4302537.31503/94‑1 (MQ=60)
                                               AAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCG                                               <  SRR4302537.911239/94‑1 (MQ=60)
                                                    ATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGC                                          >  SRR4302537.3371841/1‑94 (MQ=60)
                                                     TTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCT                                         >  SRR4302537.4257141/1‑94 (MQ=60)
                                                     TTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCT                                         <  SRR4302537.4280528/94‑1 (MQ=60)
                                                     TTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCT                                         <  SRR4302537.5022849/94‑1 (MQ=60)
                                                      TGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTG                                        <  SRR4302537.3621364/94‑1 (MQ=60)
                                                         AGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTT                                     >  SRR4302537.5136143/1‑94 (MQ=60)
                                                         AGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTT                                     >  SRR4302537.6266133/1‑94 (MQ=60)
                                                           AGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGC                                   >  SRR4302537.2551406/1‑94 (MQ=60)
                                                           AGAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGC                                   >  SRR4302537.5791728/1‑94 (MQ=60)
                                                            GAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGGGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCC                                  >  SRR4302537.264791/1‑94 (MQ=60)
                                                            GAAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCC                                  >  SRR4302537.6193335/1‑94 (MQ=60)
                                                             AAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCG                                 >  SRR4302537.2143759/1‑94 (MQ=60)
                                                             AAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCG                                 >  SRR4302537.694798/1‑94 (MQ=60)
                                                             AAAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCG                                 >  SRR4302537.4805580/1‑94 (MQ=60)
                                                              AAACCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTAACGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGA                                <  SRR4302537.106140/94‑1 (MQ=60)
                                                               AGCCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAA                               <  SRR4302537.6471234/94‑1 (MQ=60)
                                                                 CCTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGG                             >  SRR4302537.3466411/1‑94 (MQ=60)
                                                                  CTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGC                            >  SRR4302537.2084465/1‑94 (MQ=60)
                                                                  CTTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGA                            >  SRR4302537.2826409/1‑94 (MQ=60)
                                                                   TTTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCA                           >  SRR4302537.1350459/1‑94 (MQ=60)
                                                                    TTTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAA                          >  SRR4302537.3486491/1‑94 (MQ=60)
                                                                     TTACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAA                         >  SRR4302537.2133390/1‑94 (MQ=60)
                                                                      TACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAAT                        <  SRR4302537.3016750/94‑1 (MQ=60)
                                                                      TACCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAAT                        >  SRR4302537.3197870/1‑94 (MQ=60)
                                                                        CCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTT                      >  SRR4302537.3989272/1‑94 (MQ=60)
                                                                        CCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTT                      >  SRR4302537.5906032/1‑94 (MQ=60)
                                                                        CCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTT                      <  SRR4302537.3062501/94‑1 (MQ=60)
                                                                        CCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTT                      >  SRR4302537.3725016/1‑94 (MQ=60)
                                                                        CCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTT                      >  SRR4302537.5474832/1‑94 (MQ=60)
                                                                        CCCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGCGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTT                      >  SRR4302537.1112344/1‑94 (MQ=60)
                                                                         CCGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTC                     >  SRR4302537.313093/1‑94 (MQ=60)
                                                                          CGTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTCG                    <  SRR4302537.2580446/94‑1 (MQ=60)
                                                                           GTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTCGC                   >  SRR4302537.2063555/1‑94 (MQ=60)
                                                                           GTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTCGC                   >  SRR4302537.5819400/1‑94 (MQ=60)
                                                                           GTTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTCGC                   <  SRR4302537.1946189/94‑1 (MQ=60)
                                                                           GGTTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTCGC                   <  SRR4302537.1758204/94‑1 (MQ=60)
                                                                             TTGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTCGCGA                 >  SRR4302537.3156661/1‑94 (MQ=60)
                                                                              TGGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTCGCGAA                >  SRR4302537.6315576/1‑94 (MQ=60)
                                                                               GGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCTGAAGGCAAATTTCGCGAAG               <  SRR4302537.6170352/94‑1 (MQ=60)
                                                                               GGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTCGCGAAG               <  SRR4302537.4254305/94‑1 (MQ=60)
                                                                               GGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTCGCGAAG               >  SRR4302537.5181258/1‑94 (MQ=60)
                                                                               GGCAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTCGCGAAG               >  SRR4302537.6500810/1‑94 (MQ=60)
                                                                                 CAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAGCGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTCGCGAAGAT             >  SRR4302537.4589933/1‑94 (MQ=60)
                                                                                 CAGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTCGCGAAGAT             >  SRR4302537.3736679/1‑94 (MQ=60)
                                                                                  AGCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTCGCGAAGATT            >  SRR4302537.1321438/1‑94 (MQ=60)
                                                                                   GCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTCGCGAAGATCT           <  SRR4302537.141824/94‑1 (MQ=60)
                                                                                   GCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTCGCGAAGATCT           >  SRR4302537.398075/1‑94 (MQ=60)
                                                                                   GCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTCGCGAAGATCT           <  SRR4302537.2048148/94‑1 (MQ=60)
                                                                                   GCGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCAAGCTGGTTGCCGAAGGCAAATTTCGCGAAGATCT           <  SRR4302537.4398973/94‑1 (MQ=60)
                                                                                    CGATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTCGCGAAGATCTG          <  SRR4302537.2512790/94‑1 (MQ=60)
                                                                                     GATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTCGCGAAGATCTGT         <  SRR4302537.5095026/94‑1 (MQ=60)
                                                                                      ATCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTCGCGAAGATCCGTA        <  SRR4302537.3585446/94‑1 (MQ=60)
                                                                                       TCGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTCGCGAAGATCTGTAC       <  SRR4302537.5226971/94‑1 (MQ=60)
                                                                                        CGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTCGCGAAGATCTGTACG      >  SRR4302537.5402551/1‑94 (MQ=60)
                                                                                        CGCCGGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTCGCGAAGATCTGTACG      <  SRR4302537.3736751/94‑1 (MQ=60)
                                                                                            GGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTCGCGAAGGTCTGTACGCGCG  >  SRR4302537.6154965/1‑94 (MQ=60)
                                                                                            |                                                                                             
TGATGAGATTGGCGAACTGGGCGCAGACGAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCAGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTCGCGAAGATCTGTACGCGCG  >  NC_000913/3556763‑3556948

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑